Intelligent mouse drug test device

Abstract

The invention relates to the field of experimental animal behavior monitoring, in particular to an intelligent mouse drug test device which comprises a monitoring cage body, and a feeding and drinking monitoring module, a track recording module, a drug administration module and a behavior stimulation module are installed on the monitoring cage body. The administration module comprises an atomization administration assembly detachably mounted at the top of the monitoring cage body, a drinking water exposure assembly mounted on the eating and drinking water monitoring module, and a whole body aerosol exposure pipeline arranged on the inner wall of the monitoring cage body; the behavior stimulation module comprises a plurality of stimulation units which monitor the bottom of the cage body and are distributed in an array mode, and each stimulation unit is integrated with a foot sole cold and hot stimulation assembly and an electric stimulation assembly; and the controller is used for receiving signals of all the modules and controlling all the modules to execute cooperative actions. The device solves the problem of pain points in a traditional device, and provides comprehensive, accurate and continuous test data support for drug research and development.

Description

Technical Field

[0001] This invention relates to the field of laboratory animal behavior monitoring, and specifically to an intelligent mouse drug testing device. Background Technology

[0002] In drug development trials, mice are commonly used experimental animals. Their data on food and water intake, activity patterns, drug responses, and behavioral feedback to stimuli are core evidence for analyzing drug dosage metabolism and response, and for evaluating drug efficacy and safety.

[0003] Existing intelligent mouse drug testing devices suffer from several technical limitations: First, their functional modules operate independently, lacking coordinated linkage between functions such as food and water monitoring, drug administration, behavioral stimulation, and trajectory recording. This results in isolated multi-dimensional experimental data, failing to form a complete data chain and hindering in-depth correlation analysis. Second, their drug administration modes are limited, often supporting only a single route of administration, failing to meet diverse experimental needs such as nebulized drug administration, whole-body aerosol exposure, and water exposure. Furthermore, uneven drug dispersion during administration can lead to dosage waste and experimental errors. Third, the fixed stimulation area of ​​behavioral stimulation devices can easily interfere with normal physiological behaviors such as feeding and drinking in mice, and the lack of precise real-time location tracking results in insufficient stimulation targeting. These shortcomings severely impact the scientific validity, completeness, and continuity of experimental data, hindering the efficiency and accuracy of drug development trials.

[0004] Therefore, the present invention provides an intelligent mouse drug testing device to solve the above problems. Summary of the Invention

[0005] To address the aforementioned issues, this invention provides an intelligent mouse drug testing device that enables the full-chain collaboration of quantitative statistical analysis of mouse food and water intake data, multi-mode precise drug administration, multi-module targeted behavioral stimulation, and 24-hour continuous trajectory capture during drug testing. It also solves the pain points of traditional devices, providing comprehensive, accurate, and continuous experimental data support for drug development.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: An intelligent mouse drug testing device includes a monitoring cage, on which are installed a feeding and drinking monitoring module for collecting data on mouse feeding and drinking, a trajectory recording module for continuously capturing mouse activity trajectories for 24 hours, a drug delivery module for nebulized drug delivery and whole-body aerosol exposure, and a behavioral stimulation module for providing foot heat and cold stimulation, electrical stimulation, and monitoring aversion responses. The drug delivery module includes a detachably mounted nebulized drug delivery component on the top of the monitoring cage, a drinking exposure component mounted on the feeding and drinking monitoring module, and a whole-body aerosol exposure pipeline arranged on the inner wall of the monitoring cage. The behavioral stimulation module includes several stimulation units arranged in an array at the bottom of the monitoring cage, each stimulation unit integrating a foot heat and cold stimulation component and an electrical stimulation component. It also includes a controller for receiving signals from each module and controlling each module to perform coordinated actions.

[0007] When the behavior trajectory recording module detects that the mouse exhibits a preset aversion response in the controller, the controller will work with the drug delivery module to dynamically adjust the drug dosage and direction. At the same time, the trajectory recording module will mark the reaction time point and strengthen the capture of behaviors in the preset aversion response area.

[0008] When the drug delivery module initiates nebulized drug delivery or whole-body aerosol exposure, the controller, in conjunction with the trajectory recording module, adjusts the shooting parameters and simultaneously triggers the anti-contamination protection action of the food and water monitoring module.

[0009] When the feeding and drinking monitoring module detects that a mouse has entered the feeding and drinking area, the controller, in conjunction with the behavioral stimulation module, pauses the stimulation output in the feeding and drinking area, while the drug delivery module maintains the current state or switches to a safe mode.

[0010] Furthermore, the feeding and drinking monitoring module includes a food trough and a drinking device with an anti-drip valve. The food trough is installed on the side of the monitoring cage, and the drinking device is installed on the side of the food trough. The drinking device is equipped with a flow sensor. A residue collection hole is opened at the bottom of the food trough, and a removable residue box is installed below the residue collection hole. An electric scraper is installed on the inner side wall of the residue box, and a first pressure sensor for real-time monitoring of the weight of food residue is installed on the bottom wall of the residue box. Both the electric scraper and the first pressure sensor are connected to the controller signal. When the cumulative weight of residue detected by the first pressure sensor in real time exceeds the preset weight threshold in the controller, cleaning is automatically started.

[0011] Furthermore, the trajectory recording module includes several cameras and infrared sensor probes. The cameras are installed on the top and sides of the monitoring cage, and the infrared sensor probes are installed around the inner wall of the monitoring cage. Both the cameras and the infrared sensor probes are connected to the controller signal.

[0012] Furthermore, the nebulized drug delivery assembly includes a drug storage chamber, an nebulizer, and a deflectable flow guide. The drug storage chamber is fixedly connected to the monitoring cage and communicates with the nebulizer. The deflectable flow guide is detachably connected to the monitoring cage. Both the nebulizer and the deflectable flow guide are connected to the controller via signals. The whole-body aerosol exposure pipeline is evenly distributed on the inner wall of the monitoring cage. Several nozzles are opened on the side wall of the whole-body aerosol exposure pipeline. The input end of the whole-body aerosol exposure pipeline is connected to a high-pressure air pump and a drug storage tank. The whole-body aerosol exposure pipeline is equipped with an electromagnetic control valve, which is connected to the controller via signals.

[0013] Furthermore, the foot hot and cold stimulation component includes a semiconductor cooling plate, a heating plate, and a temperature sensor. The semiconductor cooling plate, heating plate, and temperature sensor are all connected to the controller via signals. The electrical stimulation component includes a flexible conductive electrode sheet. A second pressure sensor is provided on the surface of the flexible conductive electrode sheet. The second pressure sensor is used to monitor pressure changes triggered by the mouse's aversion response. Both the flexible conductive electrode sheet and the second pressure sensor are connected to the controller via signals.

[0014] Furthermore, the stimulation unit is linked with the feeding and drinking monitoring module: a weight sensing module is installed below the food trough and the drinking device. The weight sensing module is connected to the controller signal. When the weight sensing module detects the mouse feeding and drinking behavior, the controller controls the stimulation unit in the adjacent area to automatically enter the standby state. When the mouse leaves the feeding and drinking area, the stimulation unit resumes the stimulation mode according to the preset program in the controller.

[0015] Furthermore, a one-way valve is installed at the nozzle of the whole-body aerosol exposed pipeline. The one-way valve is connected to the controller signal. When the electric scraper starts to clean the residue, the controller simultaneously closes the one-way valve at the nozzle.

[0016] Furthermore, the trajectory recording module is linked with the nebulizer drug delivery component: when the trajectory recording module detects that the mouse exhibits a preset aversion response in the controller, the controller triggers the nebulizer drug delivery component to adjust the drug spray volume and adjust the angle of the deflectable shroud.

[0017] Furthermore, the electrical stimulation component and the drinking device form a linkage protection mechanism: when the electrical stimulation intensity of the electrical stimulation component exceeds the preset intensity threshold in the controller, the controller shuts off the drinking device; at the same time, the trajectory recording module marks the time point of the stimulation and performs correlation analysis with the mouse's subsequent drinking behavior data.

[0018] Furthermore, the stimulation unit is linked with the whole-body aerosol exposure pipeline: the infrared sensor detects the real-time position coordinates of the mouse in the monitoring cage and transmits them to the controller, which dynamically switches the nozzle area of ​​the whole-body aerosol exposure pipeline according to the real-time position coordinates; at the same time, the stimulation unit dynamically adjusts the activation area according to the mouse's position.

[0019] The above approach has the following beneficial effects:

[0020] 1. This solution achieves full-link collaborative operation of the drug administration module, behavioral stimulation module, trajectory recording module, and food and water monitoring module through a controller. It constructs a closed-loop experimental system that records the drug administration, the response to the drug, and the stimulation of the experimental animals in real time. Compared with the shortcomings of traditional technologies where each functional module works independently and the data is isolated and unrelated, this solution can not only accurately capture the immediate behavioral response of mice after drug administration / stimulation, but also achieve synchronous correlation of multi-dimensional data. It provides complete data support for in-depth correlation analysis between drug dosage, stimulation intensity, behavioral changes, and metabolic state, significantly improving the scientific nature and reference value of experimental data, and demonstrating the creativity of cross-module collaborative design.

[0021] 2. In this scheme, the behavioral stimulation module adopts an array of independent stimulation units, combined with an infrared sensor probe to achieve precise positioning and targeted stimulation of the mouse. The stimulation unit is linked with the feeding and drinking monitoring module, which can automatically pause the stimulation output in the feeding area. Compared with the shortcomings of traditional technology, which has a fixed stimulation area and is prone to interfering with the normal physiological behavior of mice (such as feeding and drinking), this method ensures the accuracy and effectiveness of stimulation, avoids the interference of irrelevant stimulation on experimental data, and the modular design can flexibly adapt to different experimental needs. It breaks through the limitations of traditional stimulation devices with single function and poor adaptability, and demonstrates the creativity of functional adaptability design.

[0022] 3. This solution integrates three drug delivery modes: nebulization, drinking water exposure, and whole-body aerosol exposure. It achieves targeted drug delivery through a deflectable flow guide and dynamic nozzle area switching. Compared to traditional technologies that suffer from single drug delivery modes, uneven drug dispersion, and dosage waste, this solution meets the needs of different drug formulations (liquid, aerosol) and experimental scenarios. The targeted design ensures precise drug delivery to mice, improving drug utilization while reducing experimental errors. Combined with the linkage with the trajectory recording module, it enhances behavioral capture, solving the pain point of "asynchronous drug delivery and observation" in traditional drug delivery devices, demonstrating the creativity of multi-mode precision drug delivery design.

[0023] 4. This solution incorporates a one-way valve at the nozzle of the exposed aerosol pipeline, which is linked with an electric scraper to achieve synchronous anti-clogging. Simultaneously, the food and water monitoring module is equipped with an automatic residue cleaning and anti-drip structure. Compared to traditional technologies where pipelines are easily clogged by residue and food and water are susceptible to drug contamination, this solution effectively ensures the unobstructed flow of the drug delivery pathway and the purity of the drug, avoids experimental interruptions due to contamination or clogging, reduces the frequency of manual maintenance, and significantly improves the continuity and stability of the experiment, demonstrating the creativity of the equipment's self-maintenance and anti-contamination design.

[0024] 5. This solution integrates the electrical stimulation component with the drinking device for protection. When the stimulation intensity exceeds the limit, the drinking device is automatically shut off. At the same time, the trajectory recording module marks the time point and associates it with subsequent drinking data. Compared with the shortcomings of traditional technologies, such as lack of stress response protection and susceptibility to equipment damage or data loss due to mouse agitation, this solution avoids the impact of water leakage on the cage environment and experimental data. It also provides a dedicated data dimension for the study of the correlation between stress response and drinking preference. This solution breaks through the design limitations of traditional devices that only focus on core experimental indicators and neglect auxiliary protection and data expansion, demonstrating the creativity of combining protection and data expansion.

[0025] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0026] Figure 1 This is an isometric view of an embodiment of the intelligent mouse drug testing device of the present invention;

[0027] Figure 2 This is a rear view of an embodiment of the intelligent mouse drug testing device of the present invention;

[0028] Figure 3 for Figure 2 A cross-sectional view along the AA direction.

[0029] The reference numerals in the accompanying drawings of the instruction manual include: 1. Monitoring cage; 101. Movable door; 2. Food trough; 3. Anti-drip valve; 4. Drinking device; 5. Residue box; 6. Drug storage compartment; 7. Drug storage tank; 701. Whole-body aerosol exposed pipeline. Detailed Implementation

[0030] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0031] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0032] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0033] The following detailed description illustrates the specific implementation method:

[0034] Example 1:

[0035] As attached Figures 1 to 3 The image shows an intelligent mouse drug testing device, comprising a monitoring cage 1 with an opening and a hinged movable door 101. The monitoring cage 1 is equipped with a feeding and drinking monitoring module for collecting mouse feeding and drinking data, a trajectory recording module for continuously capturing mouse activity trajectories for 24 hours, a drug delivery module for nebulized drug administration and whole-body aerosol exposure, and a behavioral stimulation module for providing foot heat and cold stimulation, electrical stimulation, and monitoring aversion responses. The drug delivery module includes a detachably mounted nebulized drug delivery component on the top of the monitoring cage 1, a drinking exposure component mounted on the feeding and drinking monitoring module, and a whole-body aerosol exposure pipeline 701 arranged on the inner wall of the monitoring cage 1. The behavioral stimulation module includes several stimulation units arranged in an array at the bottom of the monitoring cage 1, each stimulation unit integrating a foot heat and cold stimulation component and an electrical stimulation component. It also includes a controller for receiving signals from each module and controlling the modules to perform coordinated actions.

[0036] When the behavior tracking module detects a mouse exhibiting a preset aversion response in the controller, the controller, in conjunction with the drug delivery module, dynamically adjusts the drug dosage and direction. Simultaneously, the tracking module marks the reaction time point and enhances the capture of behaviors occurring in the preset aversion response area. When the drug delivery module initiates nebulized drug delivery or whole-body aerosol exposure, the controller, in conjunction with the tracking module, adjusts the recording parameters and triggers the anti-contamination protection action of the feeding and drinking monitoring module. When the feeding and drinking monitoring module detects a mouse entering the feeding and drinking area, the controller, in conjunction with the behavior stimulation module, pauses the stimulation output in the feeding and drinking area, while the drug delivery module maintains its current state or switches to a safe mode.

[0037] The feeding and drinking monitoring module includes a food trough 2 and a drinking device 4 with an anti-drip valve 3. The food trough 2 is installed on the side of the monitoring cage 1, and the drinking device 4 is installed on the side of the food trough 2. The drinking device 4 is equipped with a flow sensor. The bottom of the food trough 2 has a residue collection hole, and a detachable residue box 5 is installed below the residue collection hole. An electric scraper is installed on the inner side wall of the residue box 5, and a first pressure sensor for real-time monitoring of the weight of food residue is installed on the bottom inner wall of the residue box 5. Both the electric scraper and the first pressure sensor are connected to the controller signal. When the cumulative weight of residue detected by the first pressure sensor in real time exceeds the preset weight threshold in the controller, cleaning is automatically started.

[0038] The trajectory recording module includes several cameras and infrared sensors. The cameras are installed on the top and sides of the monitoring cage 1, and the infrared sensors are installed around the inner wall of the monitoring cage 1. Both the cameras and the infrared sensors are connected to the controller signal.

[0039] The nebulized drug delivery assembly includes a drug storage chamber 6, an nebulizer, and a deflectable flow guide. The drug storage chamber 6 is fixedly connected to the monitoring cage 1 and is also connected to the nebulizer. The deflectable flow guide is detachably connected to the monitoring cage 1. Both the nebulizer and the deflectable flow guide are connected to the controller via signals. The whole-body aerosol exposure pipeline 701 is evenly distributed on the inner wall of the monitoring cage 1. Several spray holes are opened on the side wall of the whole-body aerosol exposure pipeline 701. The input end of the whole-body aerosol exposure pipeline 701 is connected to a high-pressure air pump and a drug storage tank 7. The whole-body aerosol exposure pipeline 701 is equipped with an electromagnetic control valve, which is connected to the controller via signals.

[0040] The foot hot and cold stimulation component includes a semiconductor cooling plate, a heating plate, and a temperature sensor. The semiconductor cooling plate, heating plate, and temperature sensor are all connected to the controller. The electrical stimulation component includes a flexible conductive electrode sheet. A second pressure sensor is provided on the surface of the flexible conductive electrode sheet. The second pressure sensor is used to monitor the pressure changes triggered by the mouse's aversion response. Both the flexible conductive electrode sheet and the second pressure sensor are connected to the controller.

[0041] The stimulation unit is linked with the feeding and drinking monitoring module: a weight sensing module is installed below the food trough 2 and the drinking device 4. The weight sensing module is connected to the controller signal. When the weight sensing module detects the feeding and drinking behavior of the mouse, the controller controls the stimulation unit in the adjacent area to automatically enter the standby state. When the mouse leaves the feeding and drinking area, the stimulation unit resumes the stimulation mode according to the preset program in the controller.

[0042] The trajectory recording module is linked with the nebulizer drug delivery component: when the trajectory recording module detects that the mouse exhibits a preset aversion response in the controller, the controller triggers the nebulizer drug delivery component to adjust the drug spray volume and adjust the angle of the deflectable guide hood.

[0043] The electrical stimulation component and the drinking device 4 form a linkage protection: the anti-drip valve 3 of the drinking device 4 is equipped with a lever, one end of which is connected to a connecting rod. The output end of the electrical stimulation component is hinged to the end of the connecting rod away from the lever. When the electrical stimulation intensity of the electrical stimulation component exceeds the preset intensity threshold in the controller, the current change of the electrical stimulation component drives the connecting rod to push the lever, causing the anti-drip valve 3 to close. At the same time, the trajectory recording module marks the time point of the stimulation.

[0044] The stimulation unit is linked with the whole-body aerosol exposure tubing 701: the infrared sensor detects the real-time position coordinates of the mouse in the monitoring cage 1 and transmits them to the controller. The controller dynamically switches the nozzle area of ​​the whole-body aerosol exposure tubing 701 according to the real-time position coordinates. At the same time, the stimulation unit dynamically adjusts the activation area according to the position of the mouse.

[0045] The specific implementation process is as follows: Place the monitoring cage 1 on a horizontal test platform, ensuring that each module is firmly connected to the cage through standardized interfaces. Add a quantitative amount of granular test feed to the food trough 2, inject regular drinking water into the drinking device 4, add the test reagent of the preset concentration to the drug storage tank 6 of the atomized drug delivery component and the drug storage tank 7 of the whole-body aerosol exposure pipeline 701, and install the residue box 5 below the food trough 2.

[0046] The controller presets various test thresholds and procedures, including: cumulative residue weight threshold, electrical stimulation intensity range, foot cold and hot stimulation temperature range, mouse aversion response judgment criteria (such as the pressure threshold for violently stomping on the electrode pads, the speed threshold for rapidly escaping from a certain area), initial dose and adjustment gradient for nebulized drug delivery, and routine and enhanced shooting parameters for the camera.

[0047] Open the movable door 101 of monitoring cage 1, place the experimental mouse inside monitoring cage 1, and close the movable door 101. The controller automatically starts each module and enters the basic monitoring state. The camera and infrared sensor of the trajectory recording module start working, capturing the mouse's movement trajectory in real time and transmitting it to the controller; the flow sensor and weight sensor of the feeding and drinking monitoring module continuously monitor and record whether the mouse enters the feeding and drinking area and related data; the stimulation unit of the behavioral stimulation module is in a standby state, and the second pressure sensor monitors pressure changes in real time.

[0048] When a mouse enters the feeding and drinking area and touches the food trough 2 or the drinking device 4, the weight sensing module below the food trough 2 and the drinking device 4 detects the weight change and immediately sends a signal to the controller. Upon receiving the signal, the controller quickly puts the stimulation units adjacent to the feeding and drinking area into standby mode, pausing the output of hot / cold stimulation and electrical stimulation in that area to avoid interfering with the mouse's normal feeding and drinking behavior. When the mouse leaves the feeding and drinking area, the weight sensing module detects that the weight has returned to its initial value, and the controller instructs the stimulation units to resume the stimulation mode according to the preset program to continue the experiment.

[0049] According to the experimental protocol, the controller initiates either nebulized drug delivery or whole-body aerosol exposure mode. If nebulized drug delivery is initiated, the drug storage tank 6 is connected to the nebulizer, which atomizes the drug and sprays it into the cage through a deflectable hood. Simultaneously, the controller, in conjunction with the trajectory recording module, adjusts the camera's shooting parameters, increases the frame rate, and optimizes the shooting angle to ensure clear capture of the mouse's behavioral responses during drug delivery. If whole-body aerosol exposure is initiated, the electromagnetic control valve opens, and the high-pressure air pump pushes the drug from the drug storage tank 7 to the whole-body aerosol exposure pipeline 701, which then evenly diffuses the drug into the monitoring cage 1 through side-wall nozzles, simultaneously triggering parameter adjustments in the trajectory recording module.

[0050] During the experiment, if the trajectory recording module detects a preset aversion response in the mouse (such as the mouse rapidly escaping as captured by the infrared sensor, or the second pressure sensor detecting a stepping pressure exceeding a threshold), the controller immediately triggers the adjustment action of the nebulizer drug delivery component: based on the mouse's real-time position data, the deflection angle of the deflectable guide hood is adjusted so that the nebulized drug is precisely targeted to the area where the mouse is located, and the drug spray volume is adjusted according to the preset gradient; the trajectory recording module simultaneously marks the time point of the response, the camera focuses on the mouse's location and switches to enhanced shooting parameters to record the behavioral details of the aversion response in detail.

[0051] When the experiment requires activation of the electrical stimulation function, the controller outputs an electrical signal of a specified intensity to the flexible conductive electrode pads according to a preset program. If the electrical stimulation intensity exceeds a preset threshold, the current change in the electrical stimulation component drives the linkage to move. The linkage pushes the latch on the anti-drip valve 3 of the drinking device 4, causing the valve to close quickly and preventing leakage caused by the mouse colliding with the drinking device 4 due to stress response. At the same time, the trajectory recording module automatically marks the precise time point of the stimulation, and subsequently associates and stores this time point with the mouse's drinking behavior data.

[0052] Infrared sensors continuously monitor the real-time position coordinates of mice within their cages and transmit this data to the controller. Based on this position data, the controller dynamically switches the activation area of ​​the nozzles in the whole-body aerosol exposure tubing 701, ensuring that the aerosol nozzles always face the mouse's activity area and guaranteeing precise contact with the experimental agent. Simultaneously, the controller instructs the stimulation units of the behavioral stimulation module to dynamically adjust their activation areas, activating only the stimulation units corresponding to the mouse's paws. This achieves a synergy between targeted exposure and precise stimulation. The trajectory recording module tracks and records the mouse's trajectory changes and behavioral responses throughout the process.

[0053] During the experiment, food scraps spilled by the mice fell into the scrap box 5 through the scrap collection hole at the bottom of the food trough 2. A first pressure sensor on the bottom wall of the scrap box 5 monitored the accumulated weight of the scrap in real time. When the accumulated weight exceeded a preset threshold, the first pressure sensor sent a cleaning signal to the controller. The controller then activated the electric scraper, which slid along the bottom surface of the scrap box 5 to push the scrap to one end of the box for centralized storage, preventing the accumulation of scrap from affecting the working accuracy of each module.

[0054] After the preset duration of the experiment, the controller shuts down all modules and opens the movable door 101 of the monitoring cage 1 to remove the mouse. The residue box 5 is disassembled for cleaning, and food, water, and experimental reagents are replenished or replaced in preparation for the next experiment. The controller's export function is used to export complete data recorded during the experiment, including feeding and drinking data, trajectory data, drug administration data, stimulus data, and aversion response data. This data is then used for subsequent correlation analysis, from drug dosage to metabolism, and finally, the resulting responses in the experimental mice.

[0055] Example 2:

[0056] As attached Figure 1 As shown, the difference from Embodiment 1 is that a one-way valve is provided at the nozzle of the whole-body aerosol exposure pipeline 701. The one-way valve is connected to the controller signal. When the electric scraper starts to clean the residue, the controller simultaneously closes the one-way valve at the nozzle.

[0057] The specific implementation process is as follows: reset the one-way valve of the whole-body aerosol exposure pipeline 701 to the open state, ensure that the pipeline is connected normally to the high-pressure air pump and the drug storage tank 7, and at the same time install the residue box 5 in place, so that the first pressure sensor is in real-time monitoring state, and establish a signal connection between all components and the controller.

[0058] During the experiment, food residue from the mice fell into the residue box 5 through the collection hole at the bottom of the food trough 2. The first pressure sensor continuously collected the weight data of the residue and transmitted it to the controller. When the accumulated weight of the residue exceeded a preset threshold, the controller immediately sent a start command to the electric scraper and a close command to the one-way valve. Upon receiving the signal, the one-way valve quickly closed, preventing dust and residue in the residue box 5 from entering the nozzle of the aerosol exposure pipeline. At the same time, the electric scraper slid along the bottom surface of the residue box 5, pushing the accumulated residue to one end of the box for centralized storage, preventing the residue from spreading. After the electric scraper completed the cleaning action, it sent a completion signal to the controller, which then sent a command to reopen the one-way valve, restoring normal ventilation to the whole-body aerosol exposure pipeline 701, ensuring that subsequent drug administration was not affected.

[0059] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. An intelligent mouse drug testing device, comprising a monitoring cage (1) on which an eating and drinking monitoring module for counting mouse eating and drinking data, a trajectory recording module for continuously capturing mouse activity trajectory for 24 hours, a drug administration module for realizing atomization administration and whole-body aerosol exposure, and a behavior stimulation module for providing plantar cold and hot stimulation, electric stimulation and monitoring aversive reaction are installed, characterized in that, The administration module comprises an atomization administration assembly detachably mounted on the top of the monitoring cage (1), a drinking water exposure assembly mounted on the food and water intake monitoring module, and a whole-body aerosol exposure pipeline (701) arranged on the inner wall of the monitoring cage (1); the behavior stimulation module comprises a plurality of stimulation units arranged in an array at the bottom of the monitoring cage (1), each stimulation unit is integrated with a foot cold and hot stimulation assembly and an electric stimulation assembly; and the controller for receiving signals of each module and controlling the modules to perform cooperative actions is further included; When the behavior trajectory recording module detects that the mouse appears a preset aversive reaction in the controller, the controller links the administration module to dynamically adjust the administration dose and direction, and at the same time, the trajectory recording module marks the reaction time point and strengthens the behavior capture in the area where the preset aversive reaction appears; When the administration module starts atomization administration or whole-body aerosol exposure, the controller links the trajectory recording module to adjust the shooting parameters, and at the same time, triggers the anti-pollution protection action of the food and water intake monitoring module; When the food and water intake monitoring module detects that the mouse enters the food and water intake area, the controller links the behavior stimulation module to suspend the stimulation output in the food and water intake area, and at the same time, the administration module maintains the current state or switches to the safe mode.

2. The intelligent mouse drug testing device of claim 1, wherein, The food and water intake monitoring module comprises a food trough (2) and a drinking water device (4) with a drip-proof valve (3), the food trough (2) is mounted on the side of the monitoring cage (1), the drinking water device (4) is mounted on one side of the food trough (2), the drinking water device (4) is provided with a flow sensor, a residue collection hole is formed in the bottom of the food trough (2), a detachable residue box (5) is mounted below the residue collection hole, an electric residue scraping plate is mounted on the inner side wall of the residue box (5), a first pressure sensor for monitoring the weight of food residues in real time is mounted on the inner bottom wall of the residue box (5), and the electric residue scraping plate and the first pressure sensor are both signal-connected with the controller, and the cleaning is automatically started when the accumulated weight of residues checked by the first pressure sensor in real time exceeds the preset weight threshold in the controller.

3. The intelligent mouse drug testing device of claim 2, wherein, The trajectory recording module comprises a plurality of cameras and infrared sensing probes, the cameras are respectively mounted on the top and both sides of the monitoring cage (1), and the infrared sensing probes are mounted on the inner wall of the monitoring cage (1) around.

4. The intelligent mouse drug testing device of claim 3, wherein, The atomization administration assembly comprises a medicine storage bin (6), an atomizer and a deflectable flow guide cover, the medicine storage bin (6) is fixedly connected with the monitoring cage (1), and the medicine storage bin (6) is in communication with the atomizer, the deflectable flow guide cover is detachably connected with the monitoring cage (1), and the atomizer and the deflectable flow guide cover are both signal-connected with the controller; the whole-body aerosol exposure pipeline (701) is uniformly distributed on the inner wall of the monitoring cage (1), a plurality of spray holes are formed in the side wall of the whole-body aerosol exposure pipeline (701), the input end of the whole-body aerosol exposure pipeline (701) is connected with a high-pressure gas pump and a medicine storage tank (7), the whole-body aerosol exposure pipeline (701) is provided with an electromagnetic control valve, and the electromagnetic control valve is signal-connected with the controller.

5. The intelligent mouse drug testing device of claim 4, wherein, The plantar cold and hot stimulation assembly includes a semiconductor refrigeration sheet, a heating sheet, and a temperature sensor, all of which are signal-connected with the controller; the electric stimulation assembly includes a flexible conductive electrode sheet, the surface of which is provided with a second pressure sensor for monitoring the pressure change triggered by the mouse aversion reaction, both the flexible conductive electrode sheet and the second pressure sensor being signal-connected with the controller.

6. The intelligent mouse drug testing device of claim 5, wherein, The stimulation unit is linked with the food and water consumption monitoring module: the food trough (2) and the water drinking device (4) are provided below with a weight sensing module, which is signal-connected with the controller; when the weight sensing module detects the mouse food and water consumption behavior, the controller controls the stimulation unit in the adjacent area to automatically enter the standby state; when the mouse leaves the food and water consumption area, the stimulation unit resumes the stimulation mode according to the preset program in the controller.

7. The intelligent mouse drug testing device of claim 6, wherein, The one-way valve is signal-connected with the controller, and when the electric slag scraping plate starts to clean the residues, the controller synchronously closes the one-way valve at the spray hole of the whole-body aerosol exposure pipeline (701).

8. The intelligent mouse drug testing device of claim 7, wherein, The trajectory recording module and the atomization drug delivery assembly are linked through the controller: when the trajectory recording module detects that the mouse appears the preset aversion reaction in the controller, the controller triggers the atomization drug delivery assembly to adjust the drug injection amount and the angle of the deflectable flow guide cover.

9. The intelligent mouse drug testing device of claim 8, wherein, The electric stimulation assembly and the water drinking device (4) are linked through the controller: the water drinking device (4) is provided with a stirring piece on the anti-dripping valve (3), one end of the stirring piece is connected with a connecting rod, and the output end of the electric stimulation assembly is hinged with the connecting rod away from the stirring piece; when the electric stimulation intensity of the electric stimulation assembly exceeds the preset intensity threshold in the controller, the current change of the electric stimulation assembly drives the connecting rod to push the stirring piece, so that the anti-dripping valve (3) is closed; at the same time, the trajectory recording module marks the stimulation time point.

10. The intelligent mouse drug testing device of claim 9, wherein, The stimulation unit and the whole-body aerosol exposure pipeline (701) are linked through the controller: the infrared sensing probe detects the real-time position coordinates of the mouse in the monitoring cage (1) and transmits them to the controller, the controller dynamically switches the spray hole area of the whole-body aerosol exposure pipeline (701) according to the real-time position coordinates; at the same time, the stimulation unit dynamically adjusts the activation area according to the position of the mouse.

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