Process for the enzymatic preparation of butanediamine and spermidine
By using an enzymatic cascade reaction with the arginine decarboxylase mutant adiAH730D-E467K-H736E, the problems of low conversion rate and high cost in spermidine production have been solved, realizing the potential for efficient industrial application of spermidine preparation.
CN121801883BActive Publication Date: 2026-06-02JIANGNAN UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- JIANGNAN UNIV
- Filing Date
- 2026-03-12
- Publication Date
- 2026-06-02
Smart Images
Figure CN121801883B_ABST
Abstract
The application discloses a method for preparing putrescine and spermidine by using an enzyme, and belongs to the field of bioengineering.The application successfully provides a novel arginine decarboxylase mutant, the mutant exhibits excellent catalytic performance under alkaline conditions, and the specific enzyme activity reaches 24.9 U / g, which is 8.3 times higher than that of a wild-type enzyme.Based on the constructed arginine decarboxylase mutant, the application develops a two-step synthesis process for efficiently preparing spermidine.The experimental results show that the process successfully realizes significant improvement of the yield of spermidine, and the final yield reaches 200.4 mg / L.
Need to check novelty before this filing date? Find Prior Art