Dual synergistic anti-aging and anti-inflammatory composition and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and discloses a dual synergistic anti-aging and anti-inflammatory composition and application thereof. The composition disclosed by the invention is prepared from benzylidene dimethoxy dimethyl indanone and a radix pseudostellariae extract, the mass ratio of the benzylidene dimethoxy dimethyl indanone to the radix pseudostellariae extract is (1: 0.01) to (1: 500000). By combining the benzylidene dimethoxy dimethyl indanone and the radix pseudostellariae extract, extracellular matrix and senescent cell related secretion phenotypes can be effectively improved, cell damage and senescence caused by UVA and UVB can be improved, expression of collagen and elastin can be promoted, expression of matrix metalloproteinase can be inhibited, expression of proinflammatory factors can be inhibited, and the anti-aging effect can be achieved. And the compound shows good anti-aging and anti-inflammatory effects at a relatively low concentration.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, and specifically relates to a composition with dual synergistic anti-aging and anti-inflammatory effects and its application. Background Technology

[0002] The essence of skin aging is cellular aging. Studies have shown that extracellular matrix homeostasis imbalance and chronic inflammation are important biological markers of skin aging. As a key supporting structure of the skin, the extracellular matrix not only provides physical protection and structural support for dermal cells but also plays a dynamic regulatory role in physiological processes such as intercellular signaling, cell growth and differentiation regulation, and tissue repair. The skin extracellular matrix is ​​mainly composed of molecules such as collagen, elastin, laminin, and hyaluronic acid. With the progression of aging, skin tissues and cells secrete large amounts of extracellular matrix degrading enzymes such as matrix metalloproteinases (MMPs). These enzymes accelerate the degradation of collagen and elastin, leading to decreased skin tissue support and loss of elasticity, thus causing aging phenotypes such as sagging skin and wrinkles. The degradation of the extracellular matrix disrupts the skin cell microenvironment, which directly affects normal intercellular communication. Senescent cells are activated and exhibit age-associated secretory phenotypes (SASPs), continuously releasing large amounts of inflammatory factors (such as IL-6, IL-8, and TNF-α) and proteases (such as MMPs). These substances enhance their own aging state through autocrine secretion, and also affect neighboring cells through paracrine effects, accelerating the aging process of surrounding cells and creating an "aging amplification effect," which further exacerbates skin aging.

[0003] The regulatory mechanisms of skin aging are complex and influenced by multiple factors, including genetics, environment, and metabolism. Effective anti-aging results require synergistic intervention across multiple pathways and targets. While current mainstream anti-aging ingredients (such as collagen, peptides, and retinol) offer some efficacy in specific anti-aging dimensions, they often focus on a single pathway, making it difficult to achieve multi-dimensional synergistic regulation of the aging process and comprehensively address the core pathological aspects of skin aging.

[0004] Improving the imbalance of extracellular matrix degradation and regulating the abnormal activation of age-related secretory phenotypes (SASPs) are crucial pathways for intervening in skin aging. This is especially true for individuals with sensitive skin seeking anti-aging benefits, whose skin faces the dual challenges of aging and barrier damage. Conventional anti-aging ingredients may exacerbate skin sensitivity, while simple soothing ingredients are insufficient to meet their anti-aging needs. Therefore, identifying effective ingredients or combinations that can simultaneously improve extracellular matrix homeostasis and regulate SASP secretion not only precisely addresses the anti-aging challenges of sensitive skin but also provides a new scientific direction for the development of anti-aging skincare products. This has significant theoretical and practical implications for promoting the precision and scientific development of the anti-aging skincare field. Summary of the Invention

[0005] The present invention aims to at least solve one of the technical problems existing in the prior art. Therefore, the objective of the present invention is to provide a composition with dual synergistic anti-aging and anti-inflammatory effects and its application; the composition of the present invention can effectively improve the extracellular matrix and senescent cell-related secretory phenotypes, improve cell damage and aging caused by UVA and UVB, promote collagen and elastin expression, inhibit matrix metalloproteinase expression, inhibit pro-inflammatory factor expression, and exhibit good anti-aging and anti-inflammatory effects at low concentrations.

[0006] In a first aspect, the present invention provides a composition comprising benzenemethylenedimethoxydimethylindanone and Codonopsis pilosula extract; wherein the mass ratio of benzenemethylenedimethoxydimethylindanone to Codonopsis pilosula extract is from 1:0.01 to 1:500000.

[0007] Specifically, the *Pseudostellaria heterophylla* in the composition of this invention is a perennial herb belonging to the genus *Pseudostellaria* of the Caryophyllaceae family, possessing the effects of invigorating qi and strengthening the spleen, promoting body fluids and moistening the lungs. In the field of traditional Chinese medicine, *Pseudostellaria heterophylla* is widely used to treat symptoms such as spleen deficiency and fatigue, loss of appetite, weakness after illness, qi and yin deficiency, and dry cough due to lung dryness. It is widely used in traditional Chinese medicine preparations (such as digestive tablets) and dietary therapy. *Pseudostellaria heterophylla* contains a variety of rich active ingredients, including polysaccharides, amino acids, cyclic peptides, glycosides, and volatile oils. Studies have shown that it has various pharmacological effects such as lowering blood sugar, regulating blood lipids, myocardial protection, immune regulation, antioxidation, anti-stress, anti-fatigue, and anti-tumor. In the field of dermatology, *Pseudostellaria heterophylla* extract and its cyclic peptide components have been proven to inhibit tyrosinase activity and have skin whitening and brightening effects (e.g., Reference: Li Jun, et al. Shizhen Guoyi Guoyao, 2019, 30(05):1100-1102.).

[0008] Specifically, the benzyl dimethoxydimethyl indanone (E / Z-2-benzylidene-5,6-dimethoxy-3,3-dimethylindan-1-one, CAS: 924626-15-3) in the composition of this invention is an antagonist of aromatic hydrocarbon receptors. The invention patent with publication number EP3097905A1 discloses that this component can be used as an anti-pollution agent to reduce or prevent air pollution-induced gene expression and skin damage.

[0009] Currently, no literature has been found regarding the effects of combining Codonopsis pilosula extract with benzyldimethoxydimethylindanone on anti-aging and SASPs. This invention combines Codonopsis pilosula extract and benzyldimethoxydimethylindanone in an appropriate mass ratio and found that they exhibit strong synergistic effects in improving anti-aging, regulating the extracellular matrix, and improving the secretory phenotype of senescent cells. They also show synergistic enhancement in anti-aging and anti-inflammatory effects, regulating the secretory phenotype of senescent cells and improving the extracellular matrix. Specifically, they synergistically promote the expression of type I / III collagen and elastin, and inhibit the expression of MMP1 / 3, IL6, TNFα, and COX2.

[0010] In some embodiments of the present invention, the mass ratio of benzenemethylenedimethoxydimethylindanone to Codonopsis pilosula extract is from 1:0.1 to 1:5000. This mass ratio can be any point value or any two points within the range of 1:0.1 to 1:5000, such as 1:0.1, 1:1, 1:10, 1:100, 1:500, 1:1000, or 1:5000.

[0011] In some embodiments of the present invention, the concentration of the benzenemethylenedimethoxydimethylindanone in the composition is ≥0.001 μg / mL, preferably ≥0.01 μg / mL, and more preferably ≥0.1 μg / mL.

[0012] In some embodiments of the present invention, the concentration of the benzenemethylenedimethoxydimethylindanone in the composition is 0.1~5 μg / mL. This concentration can be any point value or any two points within the range of 0.1~5 μg / mL, such as 0.1 μg / mL, 1 μg / mL, 2 μg / mL, 3 μg / mL, 4 μg / mL, or 5 μg / mL.

[0013] In some embodiments of the present invention, the concentration of the Codonopsis pilosula extract in the composition is ≥0.01 μg / mL, preferably ≥0.1 μg / mL.

[0014] In some embodiments of the present invention, the concentration of the Codonopsis pilosula extract in the composition is 0.1 to 5000 μg / mL. This concentration can be any point value or any two-point range between 0.1 and 5000 μg / mL, for example, 0.1 μg / mL, 5 μg / mL, 10 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, 300 μg / mL, 400 μg / mL, 500 μg / mL, or 5000 μg / mL.

[0015] A second aspect of the invention provides the use of the composition described in the first aspect of the invention in the preparation of cosmetics.

[0016] In some embodiments of the present invention, the cosmetic has at least one of the following effects (1) to (5): (1) anti-aging; (2) promoting the expression of collagen and / or elastin; (3) inhibiting the expression of matrix metalloproteinases; (4) anti-inflammatory; (5) inhibiting the expression of pro-inflammatory factors.

[0017] In some embodiments of the present invention, the collagen includes type I collagen and / or type III collagen.

[0018] In some embodiments of the present invention, the matrix metalloproteinase includes matrix metalloproteinase 1 (MMP1) and / or matrix metalloproteinase 3 (MMP3).

[0019] In some embodiments of the present invention, the pro-inflammatory factor includes at least one of IL6, COX2 and TNFα.

[0020] A third aspect of the present invention provides a cosmetic product comprising the composition described in the first aspect of the present invention.

[0021] In some embodiments of the present invention, the cosmetic further includes excipients; the excipients include at least one of moisturizers, emulsifiers, skin moisturizers, preservatives, antioxidants, thickeners, and pH adjusters.

[0022] In some embodiments of the present invention, the content of the composition in cosmetics is ≤40wt%, preferably ≤20wt%, and more preferably ≤10wt%.

[0023] In some embodiments of the present invention, the composition is present in a cosmetic at a concentration of 0.1 to 6 wt%. This concentration can be any point value or any two-point range between 0.1 and 6 wt%, for example, 0.1 wt%, 0.5 wt%, 1 wt%, 2 wt%, 3 wt%, 4 wt%, 5 wt%, and 6 wt%.

[0024] In some embodiments of the present invention, the cosmetic includes lotion, cream, mask, serum or spray.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) The formulation of the composition of the present invention contains benzenemethylenedimethoxydimethylindanone and Codonopsis pilosula extract. Through such a unique formulation and scientific ratio, the two have a good synergistic effect. It has a significant synergistic effect in regulating the extracellular matrix and improving aging, especially in UVA-induced photoaging. It can promote the expression of type I / III collagen and elastin, and inhibit the expression of matrix metalloproteinase 1 / 3. Moreover, the effect of the composition is significantly better than that of benzenemethylenedimethoxydimethylindanone or Codonopsis pilosula extract alone.

[0026] (2) The composition of the present invention has a significant synergistic effect in anti-inflammatory and soothing, especially in improving the inflammatory damage caused by UVB. It can inhibit the expression of pro-inflammatory molecules IL6, COX2 and TNFα, and the effect of the composition is significantly better than that of single benzenemethylenedimethoxydimethylindanone or Codonopsis pilosula extract.

[0027] (3) The composition of the present invention has a low effective concentration, which can effectively reduce the irritation risk caused by high doses of a single component (benzyl dimethoxydimethyl indanone or Codonopsis pilosula extract); at the same time, the preparation method of the composition is simple and easy (the two can be mixed), the raw materials are readily available, and it can be produced on a large scale, with good potential for cosmetic applications. Detailed Implementation

[0028] To enable those skilled in the art to more clearly understand the technical solutions described in this invention, the following embodiments are provided for illustration. It should be noted that the following embodiments do not constitute a limitation on the scope of protection claimed by this invention.

[0029] Unless otherwise specified, the raw materials, reagents, and apparatus used in the following examples can be obtained from conventional commercial sources or by existing known methods.

[0030] Example 1 This embodiment provides a composition consisting of benzenemethylenedimethoxydimethylindanone and Codonopsis pilosula extract.

[0031] Comparative Example 1 This comparative example provides a single component, namely, Codonopsis pilosula extract.

[0032] Comparative Example 2 This comparative example provides a single component, namely benzenemethylenedimethoxydimethylindanone.

[0033] Comparative Example 3 This comparative example provides a composition consisting of Codonopsis pilosula extract and glucosylrutin.

[0034] Comparative Example 4 This comparative example provides a composition comprising benzenemethylenedimethoxydimethylindanone and tremella extract.

[0035] Comparative Example 5 This comparative example provides a composition comprising benzenemethylenedimethoxydimethylindanone and licorice extract.

[0036] Comparative Example 6 This comparative example provides a composition comprising benzenemethylenedimethoxydimethylindanone and ginseng extract.

[0037] Experimental Example 1: UVA-induced photoaging of fibroblasts 1.1 Preparation of sample solution Using DMSO and deionized water as solvents, sample solutions containing different mass concentrations of the composition of Example 1 (benzyl dimethoxydimethyl indanone and Codonopsis pilosula extract) were prepared, including 0.1 / 1 / 5 μg / mL of benzyl dimethoxydimethyl indanone mixed with 0.01 / 0.1 / 1 / 10 / 100 / 500 μg / mL of Codonopsis pilosula extract, respectively, for testing.

[0038] Using deionized water as a solvent, Comparative Example 1 (Codonopsis pilosula extract) was prepared into sample solutions with mass concentrations of 0.01 μg / mL, 0.1 μg / mL, 1 μg / mL, 10 μg / mL, 100 μg / mL, and 500 μg / mL for testing.

[0039] Using DMSO as a solvent, Comparative Example 2 (benzyl dimethoxydimethyl indanone) was prepared into sample solutions with mass concentrations of 0.1 μg / mL, 1 μg / mL, and 5 μg / mL for testing.

[0040] Using DMSO and deionized water as solvents, the composition of Comparative Example 3 (glucosylrutin and Codonopsis pilosula extract) was prepared into sample solutions containing components of different mass concentrations, including 0.1 / 1 / 5 μg / mL glucosylrutin mixed with 0.1 / 10 / 100 μg / mL Codonopsis pilosula extract, and the results were tested.

[0041] Using DMSO and deionized water as solvents, the composition of Comparative Example 4 (benzyl dimethoxydimethyl indanone and Tremella fuciformis extract) was prepared into sample solutions containing components of different mass concentrations, including 0.1 / 1 / 5 μg / mL of benzyl dimethoxydimethyl indanone mixed with 0.1 / 10 / 100 μg / mL of Tremella fuciformis extract, and the results were tested.

[0042] Using DMSO and deionized water as solvents, the composition of Comparative Example 5 (benzyl dimethoxydimethyl indanone and licorice extract) was prepared into sample solutions containing components of different mass concentrations, including 0.1 / 1 μg / mL of benzyl dimethoxydimethyl indanone mixed with 0.1 / 10 / 100 μg / mL of licorice extract, and the experiments were conducted.

[0043] 1.2 Experimental Principle Ultraviolet radiation is a major cause of skin aging and damage. UVA can penetrate the epidermis and act on the dermis, causing photoaging, which manifests as skin damage, sagging, and wrinkles. Dermal fibroblasts are the main cells that secrete and produce the extracellular matrix. Therefore, by irradiating dermal fibroblasts with UVA to induce an aging cell model, real-time quantitative PCR (RT-PCR) was used to measure extracellular matrix-related genes (such as collagen, elastin, and matrix metalloproteinases) to evaluate the anti-photoaging effects of different test substances.

[0044] 1.3 Experimental Methods (1) Cell culture and treatment Fibroblasts were cultured in DMEM complete medium (DMEM medium + 10% FBS + antibiotics) in a carbon dioxide incubator at 37°C and 5% CO2, and passaged every 2-3 days.

[0045] Fibroblasts were trypsin-digested and seeded in 6 / 12-well plates. After cell attachment, different test substances were added and incubated for 24 hours. The culture medium was then replaced with DPBS buffer, followed by UVA irradiation treatment at a dose of 10 J / cm². 2 (6mV / cm) 2 (Irradiation intensity). After irradiation, the medium was replaced with one containing the test substance and the treatment continued for 24 hours. Cells were then collected for subsequent measurements.

[0046] (2) Cell RNA extraction Cell lysis was performed using Trizol. Chloroform (1:5 volume ratio) was added to the lysis buffer, and the mixture was shaken thoroughly. After standing at room temperature for 5 min, it was centrifuged at 12000 rpm for 15 min at 4°C. The colorless supernatant was transferred to a new 1.5 mL centrifuge tube, and an equal volume of isopropanol was added. After mixing, the mixture was allowed to stand at room temperature for 10 min. The tube was then centrifuged at 12000 rpm for 10 min at 4°C, and the supernatant was discarded. 500 µL of 75% ethanol was added. The tube was then centrifuged at 7500 rpm for 5 min at 4°C, and the supernatant was discarded. The tube was allowed to air dry at room temperature for 1–2 min. Finally, an appropriate amount of DEPC water was added to dissolve the RNA precipitate completely.

[0047] (3) cDNA synthesis and RT-PCR The reverse transcription kit and quantitative real-time PCR kit used in this experiment were purchased from Nanjing Novizan Biotechnology Co., Ltd. The procedures were followed according to the kit instructions, and the expression of relevant genes was calculated using a relative quantitative method.

[0048] 1.4 Experimental Results The results are shown in Tables 1, 2, 3, 4, and 5.

[0049] Table 1

[0050] Specifically, elastin is a major component of the skin's elastic fibers, helping the skin maintain its firmness and elasticity. With age or when the skin is exposed to external stimuli, such as ultraviolet radiation, elastin gradually degrades, hardens, and denatures, resulting in a decrease in its elasticity and support. As the skin loses the support of elastic fibers, it begins to sag and droop, leading to blurred facial contours, deepened nasolabial folds, and loose skin on the neck. Therefore, elastin, as an important component of the extracellular matrix, plays a crucial role in skin aging.

[0051] As shown in Table 1, benzyl dimethoxydimethyl indanone (Comparative Example 2) had a poor effect on increasing elastin expression at concentrations of 0.1-5 μg / mL, while Codonopsis pilosula extract (Comparative Example 1) showed a certain effect on increasing elastin expression at concentrations of 0.1-500 μg / mL. Example 1 effectively increased elastin expression at mass ratios ranging from 1:0.02 to 1:5000, and its effect was superior to that of the single components (Comparative Example 1 and Comparative Example 2) at the same concentrations, demonstrating significant synergistic effects. Glucosylrutin, as an antioxidant in this field, has excellent UV absorption and free radical scavenging capabilities, and can effectively improve skin photoaging. Tremella fuciformis extract and licorice extract have good antioxidant, anti-inflammatory, and soothing effects, and can improve skin aging and sensitivity. The components in Example 1 were replaced with three other components with similar effects in the art to form Comparative Examples 3, 4, and 5. The results showed that, at the same addition amount, the effect of Example 1 on improving elastin was significantly better than that of Comparative Examples 3, 4, and 5, indicating that benzenemethylenedimethoxydimethylindanone needs to be combined with Codonopsis pilosula extract to exert a synergistic effect.

[0052] Table 2

[0053] Specifically, type I collagen is the main component of the dermis, located mostly in the deep dermis, accounting for more than 80% of the total collagen in the skin. Its fibers are large and bundled. Type I collagen forms the extracellular matrix network structure, which supports the firmness of the skin and makes it strong. With age, type I collagen is lost, the network gradually loosens and breaks down, the skin loses its support, and signs of aging such as wrinkles, sagging, and drooping appear.

[0054] As shown in Table 2, under UVA induction conditions, Comparative Example 2 showed no significant improvement in type I collagen expression at concentrations of 0.1-5 μg / mL, while Comparative Example 1 showed a certain improvement at concentrations of 0.1-500 μg / mL, with an improvement rate of 16.12% at 500 μg / mL. Example 1 showed superior improvement in type I collagen expression at lower concentrations. Compared with the single components (Comparative Example 1 and Comparative Example 2) at the same concentrations, Example 1 showed a significantly increased improvement effect, exhibiting obvious synergistic effects. Meanwhile, Comparative Examples 3, 4, and 5 did not show any improvement in type I collagen expression at mass ratios ranging from 1:0.1 to 1:1000, and at the same concentration, the effect of Example 1 was significantly better than that of Comparative Examples 3, 4, and 5.

[0055] Table 3

[0056] Specifically, type III collagen is an important component of the extracellular matrix, mainly located in the superficial dermis. Its fibers are relatively fine and form a loose network around the cells. Type III collagen is mainly found in the skin of infants and young children and decreases with age. It plays an important role in skin elasticity and scar healing; its reduction leads to skin aging.

[0057] As shown in Table 3, Comparative Example 1 exhibited a certain enhancement effect on type III collagen expression at concentrations of 0.1-1 μg / mL, while Comparative Example 2 showed no significant improvement effect on type III collagen expression at concentrations of 0.1-5 μg / mL. Example 1, however, demonstrated a superior enhancement effect, significantly outperforming the single components (Comparative Examples 1 and 2) at the same concentrations, exhibiting a clear synergistic effect. Meanwhile, Comparative Examples 3, 4, and 5 showed no enhancement effect on type III collagen expression at mass ratios ranging from 1:0.1 to 1:1. Furthermore, the effects of Comparative Examples 3-5 at the same addition amount were significantly weaker than those of Example 1.

[0058] Table 4

[0059] Specifically, MMP1 is matrix metalloproteinase 1, which can promote the degradation of type I and type III collagen. UV irradiation can activate the expression of MMP1. Excessive MMP1 activation can disrupt the stability and order of the extracellular matrix, affect the normal function and signal transduction of skin cells, and lead to weakened skin barrier function and increased moisture loss.

[0060] As shown in Table 4, Comparative Examples 1 (0.01-100 μg / mL) and 2 (0.1-1 μg / mL) exhibited some inhibitory effects on MMP1 expression, while Example 1 showed significantly better inhibitory effects than Comparative Examples 1 and 2. Compared with single components at the same concentration, Example 1 demonstrated significant synergistic effects. Furthermore, the inhibitory effect of Example 1 on MMP1 was significantly better than that of Comparative Examples 3, 4, and 5.

[0061] Table 5

[0062] Specifically, matrix metalloproteinase 3 (MMP3) can specifically hydrolyze various extracellular matrix proteins in the skin, such as type III, IV, IX, and X collagen and denatured collagen, fibronectin, laminin, elastin, etc. At the same time, MMP3 can activate precursor proteins such as MMP1 and MMP9, forming an MMP cascade reaction network, further decomposing collagen fiber fragments, hindering the normal interaction between fibroblasts and the ECM, destroying the three-dimensional structure of the skin, and accelerating skin aging.

[0063] As shown in Table 5, Comparative Examples 1 (0.1-100 μg / mL) and 2 (0.1-1 μg / mL) exhibited some inhibitory effects on MMP3 expression, but their inhibition rates were all <20%. Example 1, however, demonstrated significantly better inhibitory effects than Comparative Examples 1 and 2, showing a clear synergistic effect compared to single components at the same concentration. Furthermore, the inhibitory effect of Example 1 on MMP3 was significantly better than that of Comparative Examples 3, 4, and 5.

[0064] The results in Tables 1-5 above show that the composition provided in Example 1 has a significant effect on improving skin cell aging induced by UVA. Specifically, it promotes the expression of extracellular matrix type I / III collagen and elastin, and inhibits the expression of MMP1 / 3 in SASPs. Furthermore, the improvement effect of Example 1 is significantly better than that of a single component at the same concentration, exhibiting a clear synergistic effect. This synergistic effect cannot be achieved by replacing other similarly effective ingredients in the art. This makes the composition of the present invention not only have a stronger effect on improving aging, but also reduces the amount of single component added, minimizing irritation and lowering costs.

[0065] Experimental Example 2: UVB-induced inflammatory damage to keratinocytes 2.1 Preparation of sample solution Using DMSO and deionized water as solvents, sample solutions containing different mass concentrations of the composition of Example 1 (benzyl dimethoxydimethyl indanone and Codonopsis pilosula extract) were prepared, including 0.001 / 0.01 / 0.1 μg / mL of benzyl dimethoxydimethyl indanone mixed with 5 / 50 / 500 / 5000 μg / mL of Codonopsis pilosula extract, respectively, for testing.

[0066] Using deionized water as a solvent, Comparative Example 1 (Codonopsis pilosula extract) was prepared into sample solutions with mass concentrations of 5 μg / mL, 50 μg / mL, 500 μg / mL, and 5000 μg / mL for testing.

[0067] Using DMSO as a solvent, Comparative Example 2 (benzyl dimethoxydimethyl indanone) was prepared into sample solutions with mass concentrations of 0.001 μg / mL, 0.01 μg / mL, and 0.1 μg / mL for testing.

[0068] Using DMSO and deionized water as solvents, the composition of Comparative Example 3 (glucosylrutin and Codonopsis pilosula extract) was prepared to contain 0.001 μg / mL glucosylrutin and 500 μg / mL Codonopsis pilosula extract, and then tested.

[0069] Using DMSO and deionized water as solvents, the composition of Comparative Example 4 (benzyl dimethoxydimethyl indanone and Tremella fuciformis extract) was prepared to contain 0.01 μg / mL of benzyl dimethoxydimethyl indanone and 5 μg / mL of Tremella fuciformis extract, and then tested.

[0070] Using DMSO and deionized water as solvents, the composition of Comparative Example 5 (benzyl dimethoxydimethyl indanone and licorice extract) was prepared into sample solutions containing components of different mass concentrations, including 0.01 / 0.1 μg / mL of benzyl dimethoxydimethyl indanone mixed with 5 / 500 μg / mL of licorice extract, and the experiments were conducted.

[0071] Using DMSO and deionized water as solvents, the composition of Comparative Example 6 (benzyl dimethoxydimethyl indanone and ginseng extract) was formulated to contain 0.01 μg / mL of benzyl dimethoxydimethyl indanone and 5 μg / mL of ginseng extract, and then tested.

[0072] 2.2 Experimental Principle UVB has weaker penetrating power than UVA, and excessive UVB exposure can damage the skin barrier, leading to sunburn and epidermal damage. Keratinocytes, as the main cells of the epidermis, secrete large amounts of pro-inflammatory factors such as TNFα, IL6 / 8, and COX2 after UVB irradiation, accelerating cell damage and skin inflammation. Therefore, a UVB inflammatory damage model was established by irradiating keratinocytes with UVB, and then real-time quantitative PCR (RT-PCR) was used to measure pro-inflammatory molecules (such as COX2, TNFα, and IL6 / 8) to evaluate the anti-inflammatory and soothing effects of different test substances.

[0073] 2.3 Experimental Methods (1) Cell culture and treatment The cells used were keratinocytes (HaCaT), and the irradiation dose was 8 mJ / cm². 2 (0.2mV / cm) 2 Irradiation intensity), other culture and treatment conditions are the same as in 1.3(1).

[0074] (2) Cell RNA extraction Same as 1.3(2).

[0075] (3) cDNA synthesis and RT-PCR Same as 1.3 (3).

[0076] 2.4 Experimental Results The results are shown in Tables 6, 7, and 8.

[0077] Table 6

[0078] Specifically, IL-6 plays a crucial regulatory role in skin inflammation. When the skin is exposed to UVB, the pro-inflammatory factor IL-6 is secreted in large quantities. On one hand, IL-6 can induce vascular endothelial cells to express chemokines such as monocyte chemoattractant protein-1 (MCP-1), attracting inflammatory cells such as monocytes and macrophages to the site of inflammation and enhancing the inflammatory response. On the other hand, IL-6 can stimulate neutrophils and macrophages to release more inflammatory mediators, such as TNFα and IL-1β, further amplifying inflammatory signals and forming an inflammatory cascade. In addition, IL-6 can enhance VEGF expression in vascular endothelial cells, promoting angiogenesis and providing more oxygen and nutrients to the site of inflammation, while also facilitating the infiltration of inflammatory cells and the diffusion of inflammatory mediators.

[0079] As shown in Table 6, Comparative Example 1 did not show any inhibitory effect on IL6 expression at concentrations of 5-5000 μg / mL. Comparative Example 2 showed some inhibitory effect on IL6 expression at concentrations of 0.001-0.1 μg / mL, but the inhibition rate was <20%. Example 1 (mass ratio 1:50-500000) showed a significantly better inhibitory effect than Comparative Examples 1 and 2. Compared with single components at the same concentration, Example 1 showed obvious synergistic effects. Ginseng extract has anti-inflammatory, anti-aging effects and belongs to the same ginseng category as Codonopsis pilosula. Replacing Codonopsis pilosula extract in Example 1 with Ginseng extract to form Comparative Example 6 showed that the effect of Example 1 was significantly better than that of Comparative Example 6 at the same concentration. Meanwhile, Comparative Examples 3, 4, and 5 showed poor inhibitory effects on IL6 expression, significantly weaker than Example 1 at the same concentration, indicating that benzenemethylenedimethoxydimethylindanone needs to be combined with Codonopsis pilosula extract to exert a synergistic effect.

[0080] Table 7

[0081] Specifically, COX2 is a key enzyme in prostaglandin synthesis. Prostaglandins, in skin inflammation, can induce vasodilation and increase vascular permeability, leading to skin redness, swelling, and heat. They also attract inflammatory cells (such as neutrophils and macrophages) to the site of inflammation, amplifying the inflammatory response. Furthermore, prostaglandins can act on nerve endings, enhancing neurotransmitter release and amplifying pain and inflammatory signals. In UV-induced skin inflammation, increased COX2 expression can promote the secretion of pro-inflammatory factors such as IL1β and IL-6 by keratinocytes, further activating immune cells and maintaining the inflammatory state.

[0082] As shown in Table 7, Comparative Examples 1 (5-500 μg / mL) and 2 (0.01-0.1 μg / mL) exhibited some inhibitory effects on COX2 expression, while Example 1 showed significantly better inhibitory effects than Comparative Examples 1 and 2. Compared with single components at the same concentration, Example 1 demonstrated significant synergistic effects. Furthermore, at the same added concentration, the inhibitory effect of Example 1 on COX2 was significantly better than that of Comparative Examples 3, 4, 5, and 6.

[0083] Table 8

[0084] Specifically, TNFα is mainly secreted by macrophages and monocytes and is one of the earliest released pro-inflammatory cytokines. It can activate the NF-κB inflammatory signaling pathway, induce the expression of inflammatory mediators such as IL1β, IL6, and COX2, and amplify the inflammatory response. Furthermore, TNFα can promote increased vascular permeability, induce inflammatory cell infiltration, leading to skin edema and exacerbated inflammation. TNFα participates in the regulation of immune cell differentiation, activation, and proliferation, playing a crucial role in maintaining immune system homeostasis.

[0085] As shown in Table 8, Comparative Example 1 (5-5000 μg / mL) had no significant inhibitory effect on TNFα expression, while Comparative Example 2 (0.001-0.1 μg / mL) showed a certain inhibitory effect on TNFα expression, with an inhibition rate of 15.90%-26.44%. Example 1 showed a significantly better inhibitory effect than Comparative Examples 1 and 2, and compared with single components at the same concentration, Example 1 exhibited obvious synergistic effects. Furthermore, the inhibitory effect of Example 1 on TNFα was significantly better than that of Comparative Examples 3, 4, 5, and 6.

[0086] The results in Tables 6-8 above show that Example 1 has a significant effect on improving UVB-induced inflammatory damage, specifically by inhibiting the expression of pro-inflammatory factors IL6, COX2, and TNFα. Furthermore, the improvement effect of Example 1 is far superior to that of a single component at the same concentration, exhibiting a significant synergistic effect that cannot be achieved by replacing other similar active ingredients in the field. The composition of this invention can not only effectively improve aging, promote collagen and elastin expression, and reduce extracellular matrix degradation, but also reduce skin inflammatory damage and improve skin salivary inflammatory processes (SASPs). Moreover, its effective concentration is low, suggesting that this composition can achieve good results in improving skin aging and inflammation with only small amounts added, demonstrating great potential for application in the fine chemical industry.

[0087] Application Example 1: Emulsion containing the composition of the present invention An emulsion containing the composition of the present invention, the specific formulation of which is shown in Table 9.

[0088] Table 9

[0089] The preparation method of the above emulsion is as follows: 1. Mix the aqueous phase raw materials and heat and stir to 80℃ to disperse them evenly; 2. Mix the oil phase raw materials and put them into the oil phase preparation pot. Heat and stir to 80°C until completely melted and homogeneous. 3. Slowly pour the oil phase into the aqueous phase while homogenizing at high speed (3000 rpm) for 5 minutes to form a fine emulsion, and keep it warm for 15 minutes. 4. Cool down to 35-45℃, add the additive phase, and stir for 10 minutes.

[0090] Application Example 2: Face Cream Containing the Composition of the Invention A face cream containing the composition of the present invention, the specific formula of which is shown in Table 10.

[0091] Table 10

[0092] The preparation method of the above face cream is as follows: 1. Mix the aqueous phase raw materials and heat and stir to 80℃ until they are evenly mixed; 2. Mix the oil phase raw materials and put them into the oil phase preparation pot. Heat and stir to 80°C until completely melted and homogeneous. 3. Quickly pour the oil phase into the aqueous phase, homogenize at high speed (3500 rpm) for 8 minutes to ensure full emulsification, and keep warm for 15 minutes; 4. Cool down to 35-45℃, add the additive phase, and stir for 15 minutes.

[0093] The cosmetics prepared according to Examples 1-2 of this invention, containing the compositions of this invention, possess excellent anti-aging effects, promote the expression of collagen (type I and type III) and elastin, inhibit the expression of matrix metalloproteinases (MMP1 and MMP3), have anti-inflammatory effects, and inhibit the expression of pro-inflammatory factors (IL6, COX2, and TNFα).

[0094] The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the embodiments described. Those skilled in the art can make various equivalent modifications or substitutions without departing from the spirit of the present invention, and these equivalent modifications or substitutions are all included within the scope defined by the claims of this application.

Claims

1. A composition, characterized in that, The composition comprises benzenemethylenedimethoxydimethylindanone and Codonopsis pilosula extract; the mass ratio of benzenemethylenedimethoxydimethylindanone to Codonopsis pilosula extract is from 1:0.01 to 1:500000.

2. The composition according to claim 1, characterized in that, The mass ratio of benzenemethylenedimethoxydimethylindanone to Codonopsis pilosula extract is from 1:0.1 to 1:5000.

3. The composition according to claim 1, characterized in that, The concentration of the benzenemethylenedimethoxydimethylindanone in the composition is ≥0.001 μg / mL.

4. The composition according to claim 1, characterized in that, The concentration of the Codonopsis pilosula extract in the composition is ≥0.01 μg / mL.

5. The use of the composition according to any one of claims 1-4 in the preparation of cosmetics.

6. The application according to claim 5, characterized in that, The cosmetic has at least one of the following effects (1) to (5): (1) anti-aging; (2) promoting the expression of collagen and / or elastin; (3) inhibiting the expression of matrix metalloproteinases; (4) anti-inflammatory; (5) inhibiting the expression of pro-inflammatory factors.

7. A cosmetic product, characterized in that, The cosmetic product includes the composition according to any one of claims 1-4.

8. The cosmetic product according to claim 7, characterized in that, The cosmetic also includes excipients; the excipients include at least one of moisturizers, emulsifiers, skin moisturizers, preservatives, antioxidants, thickeners, and pH adjusters.

9. The cosmetic product according to claim 7, characterized in that, The composition is present in cosmetics at a concentration of ≤40 wt%.

10. The cosmetic product according to claim 7, characterized in that, The cosmetics include lotions, creams, masks, serums, or sprays.

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