A pharmaceutical composition for improving postpartum health level of dairy cows and application thereof

Abstract

The application belongs to the technical field of traditional Chinese veterinary medicine, and particularly relates to a medicine composition for improving postpartum health level of dairy cows and application thereof. The medicine composition comprises specific proportion of radix paeoniae alba, ramulus cinnamomi, semen persicae, ligusticum wallichii, fructus aurantii, poria cocos and glycyrrhizae, the composition can effectively promote the recovery of forage intake and milk yield of perinatal dairy cows, and can also reduce the risk of perinatal dairy cows suffering from metritis and the like, and is beneficial to the recovery of postpartum production performance of the dairy cows.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese veterinary medicine preparations, and specifically relates to a pharmaceutical composition for improving the postpartum health of dairy cows and its application. Background Technology

[0002] The peripartum period is the most critical stage in the dairy cow's production cycle. Dairy cows experience various stresses such as calving, herding, feed changes, and energy metabolism disorders. Most dairy cows will experience one or more diseases after calving. Postpartum diseases can easily lead to decreased feed intake and milk production in dairy cows after calving. They can also increase the incidence of peripartum diseases such as delayed uterine involution, retained placenta, ketosis, and metritis. At the same time, dairy cow deaths mainly occur within 21 days after calving, which seriously threatens the healthy development of the dairy industry.

[0003] Currently, the main clinical methods for preventing peripartum diseases in dairy cows are injecting oxytocin, diclofenac, meloxicam, antibiotics, and other chemical drugs after calving. However, these drugs may cause chemical residues in milk and may also cause gastrointestinal discomfort, leading to decreased gastrointestinal digestive function or even gastric ulcers in dairy cows.

[0004] In the field of traditional Chinese veterinary medicine, there are currently drugs available on the market for treating postpartum discomfort in dairy cows. These include "Guiqi Yimu Oral Liquid," which has the effects of replenishing qi and blood, promoting blood circulation and removing blood stasis, and is mainly used to treat postpartum qi deficiency and blood stasis syndrome in dairy cows, characterized by fatigue, loss of appetite, swollen vulva, prolonged lochia, abdominal pain, or retained placenta; "Yimu Shenghua Powder," which has the effects of promoting blood circulation, removing blood stasis, warming the meridians, and relieving pain, and is mainly used to treat postpartum lochia retention and blood stasis abdominal pain; and "Buyi Qinggong Powder," which has the effects of replenishing qi and blood, promoting blood circulation and removing blood stasis, and is mainly used to treat postpartum qi and blood deficiency, retained placenta, prolonged lochia, and blood stasis abdominal pain. Although these drugs are used to prevent and treat postpartum diseases in dairy cows, their formulations and indications are mainly focused on blood stasis and abdominal pain, resulting in a narrow range of applications and a weak effect on improving the overall health of dairy cows during the peripartum period.

[0005] Therefore, developing a safe, effective, and low-side-effect veterinary Chinese medicine preparation that is highly targeted at dairy cows during the peripartum period and can significantly improve their overall health during this time is of great practical significance. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention aims to provide a pharmaceutical composition for preventing and treating postpartum diseases in dairy cows and its application. The pharmaceutical composition includes a specific ratio of white peony root, cinnamon twig, peach kernel, chuanxiong rhizome, immature bitter orange, poria cocos, and prepared licorice root. This composition can effectively promote the recovery of feed intake and milk production in peripartum dairy cows, and can also reduce the risk of peripartum diseases such as metritis in dairy cows, which is beneficial to the recovery of postpartum health and production performance in dairy cows.

[0007] To achieve the above object, on the one hand, the present invention provides a pharmaceutical composition for improving the postpartum health level of dairy cows. The composition includes white peony root, cassia twig, peach kernel, chuanxiong rhizome, immature bitter orange, poria cocos, and roasted licorice root. The mass ratio of white peony root, cassia twig, peach kernel, chuanxiong rhizome, immature bitter orange, poria cocos, and roasted licorice root is 10-40:10-13:10-13:14-20:9-13:9-13:10-13.

[0008] Further, the composition consists of white peony root, cassia twig, peach kernel, chuanxiong rhizome, immature bitter orange, poria cocos, and roasted licorice root.

[0009] Further, the mass ratio of white peony root, cassia twig, peach kernel, chuanxiong rhizome, immature bitter orange, poria cocos, and roasted licorice root is 40:13:13:20:13:13:13.

[0010] The proportion of the pharmaceutical composition in the present invention has been verified by clinical trials and is most significant in preventing and treating perinatal diseases of dairy cows and comprehensive conditioning effects. From the perspective of traditional Chinese medicine theory, this formula follows the compatibility principle of "sovereign, ministerial, adjuvant, and courier".

[0011] Sovereign drug: White peony root is bitter, sour, and slightly cold. It belongs to the liver and spleen meridians. It has the effects of nourishing blood and regulating menstruation, astringing yin and stopping sweating, and soothing the liver and relieving pain. In this formula, the dosage of white peony root is the largest. On the one hand, it can significantly supplement the state of blood deficiency and fluid deficiency in dairy cows after childbirth. At the same time, it can relieve spasm and pain, and can effectively relieve postpartum abdominal pain caused by childbirth. It is the sovereign drug.

[0012] Ministerial drug: Cassia twig is pungent, sweet, and warm in nature. It belongs to the heart, lung, and bladder meridians. It has the effects of inducing sweating to relieve the exterior syndrome, warming the channels and promoting blood circulation, helping yang to transform qi, and calming the adverse qi. In the formula, cassia twig warms the meridians and dispels cold, promotes blood circulation through the meridians on the one hand, and helps yang to transform qi and strengthen the healthy qi to eliminate pathogenic factors, and enhances the postpartum immunity of dairy cows on the other hand. It is the ministerial drug.

[0013] Adjuvant drug: Peach kernel is bitter, sweet, and flat in nature. It belongs to the heart, liver, and large intestine meridians. It has the effects of promoting blood circulation to remove blood stasis, moistening the intestines and relieving constipation, and relieving cough and asthma. Chuanxiong rhizome is pungent and warm in nature. It belongs to the liver, gallbladder, and pericardium meridians. It has the effects of promoting blood circulation and qi movement, dispelling wind and relieving pain. Immature bitter orange is bitter, pungent, and sour, and slightly cold in nature. It belongs to the spleen and stomach meridians. It has the effects of breaking qi and reducing accumulation, resolving phlegm and dispersing stagnation. In the formula, the combination of peach kernel and chuanxiong rhizome enhances the function of promoting blood circulation to remove blood stasis, and the combination of immature bitter orange and chuanxiong rhizome enhances the function of promoting blood circulation and qi movement, jointly relieving the state of blood stasis and qi stagnation in dairy cows after childbirth. It is the adjuvant drug.

[0014] Courier drug: Poria cocos is sweet, light, and flat in nature. It belongs to the heart, lung, spleen, and kidney meridians. It has the effects of promoting diuresis and percolating dampness, strengthening the spleen, and calming the mind. Roasted licorice root is sweet and flat in nature. It belongs to the heart, lung, spleen, and stomach meridians. It has the effects of invigorating the spleen and replenishing qi, relieving spasm and pain, and harmonizing various drugs. In the formula, poria cocos and roasted licorice root jointly invigorate qi and strengthen the spleen, promote appetite, and enhance the gastrointestinal digestion function of dairy cows. At the same time, roasted licorice root is sweet and can replenish qi and blood, relieve spasm and pain, and enhance the analgesic effect of the sovereign drug. Therefore, poria cocos and roasted licorice root are jointly the courier drug.

[0015] Furthermore, the preparation method of the pharmaceutical composition includes the steps of raw material pulverization, sieving (e.g., sieving through an 80-100 mesh sieve), mixing, and dispensing.

[0016] In a second aspect, the present invention provides the use of the pharmaceutical composition described in the first aspect in the preparation of products capable of improving the postpartum health of dairy cows.

[0017] Furthermore, the dosage of the pharmaceutical composition is 700-800g / cattle.

[0018] Furthermore, the product is either animal feed or medicine.

[0019] Furthermore, the improvement of postpartum health in dairy cows includes at least one of the following:

[0020] (1) Increase the amount of feed consumed, rumination time and / or feeding duration of dairy cows after calving;

[0021] (2) Increase the amount of exercise for dairy cows after calving;

[0022] (3) Improve milk yield and / or milk quality after calving;

[0023] (4) Prevention and treatment of postpartum diseases in dairy cows.

[0024] Furthermore, the milk quality includes milk protein content and / or milk fat content.

[0025] Furthermore, the prevention and treatment of postpartum diseases in dairy cows includes reducing the incidence of metritis or peripartum diseases or promoting the speed of uterine involution in postpartum dairy cows, specifically including peripartum diseases such as metritis, retained placenta, mastitis, abomasal displacement, and digestive diseases.

[0026] Compared with the prior art, the present invention has the following advantages:

[0027] (1) The present invention combines seven Chinese veterinary drugs, namely white peony root, cinnamon twig, peach kernel, chuanxiong rhizome, immature bitter orange, poria cocos and prepared licorice root, in a specific ratio to form a drug composition. Through the synergistic effect between the raw materials, it significantly promotes the improvement of the health level of dairy cows during the peripartum period.

[0028] (2) The pharmaceutical composition of the present invention can effectively promote the recovery of feed intake and milk production in periparturient dairy cows, reduce the risk of periparturient dairy cows suffering from diseases such as metritis, and is conducive to the comprehensive and effective recovery of dairy cows' postpartum health and production performance.

[0029] (3) The preparation process of the pharmaceutical composition of the present invention is simple, suitable for industrial production, and can retain the activity of the medicinal materials to the greatest extent. Attached Figure Description

[0030] Figure 1 Milk production in different groups of dairy cows 30 days postpartum in Example 1.

[0031] Figure 2 Milk production in different groups of dairy cows 30 days postpartum in Example 2.

[0032] Figure 3 The pre- and post-partum rumination times of dairy cows in different groups in Example 2.

[0033] Figure 4 Example 2: Pre- and post-partum exercise levels of dairy cows in different groups.

[0034] Figure 5 Milk production of dairy cows in the same group 30 days postpartum in Example 3.

[0035] Figure 6 The quality of DHI milk from different groups of dairy cows in the first month after calving in Example 3.

[0036] Figure 7 Results of blood ketone levels in different groups of dairy cows on the 14th day postpartum in Example 3.

[0037] Figure 8 Postpartum uterine involution scores of different groups of dairy cows in Example 3.

[0038] Figure 9 Feeding time of dairy cows in different groups before and after calving in Example 3.

[0039] Figure 10 Milk production in different groups of dairy cows 30 days postpartum in Example 4.

[0040] Figure 11 Results of blood ketone level tests on different groups of dairy cows on the 14th day postpartum in Example 4

[0041] Figure 12 A diagram illustrating the scene of administering oral medication to dairy cows after calving.

[0042] Figure 13 A diagram illustrating the postpartum care examination and blood collection process for dairy cows. Detailed Implementation

[0043] The following detailed embodiments further illustrate the concept and technical effects of the present invention to fully understand its purpose, features, and effects. Unless otherwise specified, all methods described are conventional methods. Unless otherwise specified, all materials are available from publicly available commercial sources. The illustrative embodiments and descriptions of the present invention are used to explain the invention and do not constitute an undue limitation thereof. It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other.

[0044] Example 1: Preliminary prescription screening test

[0045] The experiment was conducted at a large-scale ranch in Shaanxi Province, using three compound prescriptions to screen prescription components.

[0046] (a) Investigational drugs

[0047] Prescription 1: Astragalus membranaceus 25 parts, Angelica sinensis 8 parts, Ligusticum chuanxiong 8 parts, Leonurus japonicus 16 parts, Cinnamomum cassia 13 parts, Ziziphus jujuba 16 parts, Zingiber officinale 13 parts.

[0048] Prescription 2: Astragalus membranaceus 23 parts, Ligusticum chuanxiong 15 parts, Leonurus japonicus 15 parts, Cinnamomum cassia 12 parts, Sophora flavescens 8 parts, Cnidium monnieri 8 parts, Rheum palmatum 5 parts, Paederia scandens 15 parts.

[0049] Prescription 3: Chuanxiong 14 parts, Guizhi 9 parts, Shudi 14 parts, Zhigancao 9 parts, Ganjiang 9 parts, Dazao 7 parts, Kushen 6 parts, Shechuangzi 6 parts, Yimucao 9 parts, Xiangfu 7 parts, Jishiteng 9 parts.

[0050] Control group: No treatment received.

[0051] (II) Test Methods

[0052] Thirty-eight newborn dairy cows were selected and divided into four groups: a control group (n=10), a group with modified prescriptions from this invention (n=9), a prescription group (n=9), and a prescription group (n=10). Cows in prescription groups one, two, and three received 755g of each prescription daily for three consecutive days postpartum, while the control group received no medication. Finally, the incidence of peripartum diseases in each group was statistically analyzed to observe the effects of traditional Chinese veterinary medicine on the incidence of peripartum diseases and milk production performance in dairy cows.

[0053] (III) Test Results

[0054] Table 1 shows the disease incidence rates in dairy cows during the peripartum period (21 days postpartum). In terms of metritis incidence, prescription group 3 showed the largest decrease, reducing the incidence by 30 percentage points compared to the control group (10.00% vs 40.00%). Regarding the overall disease incidence rate, the control group had a peripartum disease incidence rate of 40.00%, prescription group 1 had 44.44%, prescription group 2 had 44.44%, and prescription group 3 had 20.00%. Prescription group 3 showed the largest decrease, reducing the incidence by 20 percentage points compared to the control group (20.00% vs 40.00%). These results indicate that prescription 3 can reduce the incidence of postpartum metritis and the overall disease incidence rate in dairy cows, improving their postpartum health.

[0055] Results of milk production performance 30 days postpartum: Figure 1As shown in the figure, milk production in all groups showed an increasing trend. The control group experienced a slower increase in milk production; both prescriptions 1 and 3 effectively increased and maintained high milk production, with prescription 3 showing a faster initial increase but greater fluctuations; the effect of prescription 2 declined in the later stages. These results indicate that prescription 3 has a certain effect on improving the milk production performance of dairy cows.

[0056] Table 1. Statistical table of peripartum disease incidence in dairy cows of different groups

[0057]

[0058] Example 2: Prescription Screening and Treatment Course Screening Trial (I)

[0059] As can be seen from the results of Example 1, prescription 3 was the most effective among the three prescriptions. Therefore, further formulation optimization experiments were conducted on prescription 3 at a large-scale ranch in Ningxia. At the same time, the scale of the experiment was expanded and the number of administrations was reduced to observe the effect of administering the medication only once at the time of calving on the prevention and treatment of peripartum diseases in dairy cows.

[0060] (a) Investigational drugs

[0061] Prescription 1: White peony root 28 parts, cinnamon twig 9 parts, motherwort 13 parts, chuanxiong rhizome 14 parts, prepared licorice root 14 parts

[0062] Prescription 2: White peony root 28 parts, cinnamon twig 9 parts, motherwort 9 parts, chuanxiong rhizome 14 parts, cyperus rhizome 7 parts, chicken dung vine 8 parts, prepared licorice root 9 parts.

[0063] Prescription 3: 40 parts white peony root, 13 parts cinnamon twig, 13 parts peach kernel, 20 parts chuanxiong rhizome, 13 parts immature bitter orange, 13 parts poria cocos, and 13 parts prepared licorice root.

[0064] Control group: No treatment received.

[0065] (II) Test Methods

[0066] Three hundred and eighty newborn dairy cows were selected and divided into four groups: a control group (n=80), a group with modified prescriptions from this invention (n=80), a prescription group (n=80), and a prescription group (n=78). Cows in prescription groups one, two, and three were administered 755g of each prescription orally once at the postpartum pasture. The control group received no treatment. Finally, the incidence of peripartum diseases in each group was statistically analyzed, and the effects of traditional Chinese veterinary medicine on the incidence of peripartum diseases, milk production performance, rumination time, and activity level were observed.

[0067] (III) Test Results

[0068] Table 2 shows the disease incidence rates in dairy cows during the peripartum period (21 days postpartum). Regarding the incidence of metritis, the incidence rate in the three prescription groups was 14.55 percentage points lower than that in the control group (17.95% vs 32.50%), representing the largest decrease and indicating a statistically significant difference. P <0.05). Regarding the overall morbidity rate, the overall morbidity rate of peripartum cattle was 46.25% in the control group, 37.50% in prescription group 1, 25.00% in prescription group 2, and 20.51% in prescription group 3. Prescription group 3 showed the largest decrease, at 25.74 percentage points (20.51% vs 46.25%), and was statistically different from the control group. P <0.01). The above results indicate that prescription three can significantly reduce the incidence and overall incidence of postpartum metritis in dairy cows and significantly improve their postpartum health.

[0069] Results of milk production performance in dairy cows 30 days postpartum: Figure 2 As shown. On the first postpartum day, the milk production of prescription three was higher than that of prescription one, prescription two, and the control group, with a statistically significant difference between prescription three and the control group ( P (<0.05), and subsequently, milk production in all groups steadily increased. Among them, prescription three had the highest milk production, which remained at a high level and showed a stable growth trend. These results indicate that prescription three has a certain effect on improving the milk production performance of dairy cows.

[0070] Results of rumination time in dairy cows from 7 days before calving to 21 days after calving: Figure 3 As shown, there was no significant difference in feeding time among the dairy cows in each prepartum group. P >0.05), but after calving, the rumination time of the dairy cows treated with prescription three remained at the highest level, and the rumination time from 2 to 21 days postpartum was significantly higher than that of the control group, with statistical differences. P <0.05). The above results indicate that Prescription 3 can effectively increase the postpartum rumination time of dairy cows and effectively improve the recovery of postpartum gastrointestinal function.

[0071] Results of dairy cow activity levels from 7 days before calving to 21 days after calving: Figure 4 As shown in the figure, the activity levels of dairy cows in each group were stable before calving, with no significant differences. However, one week postpartum, the activity levels of dairy cows in each group began to decline sharply, and then remained in a state of stable fluctuation. However, the activity level of prescription three showed the smallest decline and remained at the highest level. Moreover, there were statistically significant differences between prescription three and the control group on postpartum days 2, 3, 8, and 10 (d2, d3, d8, d10). P <0.05). The above results indicate that prescription three can significantly alleviate the sharp decline in postpartum activity levels in dairy cows, relieve postpartum discomfort, and thus promote the health of dairy cows.

[0072] Table 2. Statistical table of peripartum disease incidence in dairy cows of different groups

[0073]

[0074] Note: * indicates a significant difference between each group and the control group. P <0.05,** P <0.01, *** P <0.001,

[0075] Example 3: Prescription Screening and Dosage Screening Trials (Part Two)

[0076] As can be seen from Example 2, the treatment course is one dose postpartum. Among the three prescriptions, prescription three showed the best effect. However, in order to determine the optimal course of treatment, this experiment administered the medicine three times postpartum to observe the effects of the traditional Chinese medicine prescription on peripartum diseases in dairy cows. The experiment was conducted at a large-scale ranch in Heilongjiang Province.

[0077] (a) Investigational drugs

[0078] Prescription 1: White peony root 28 parts, cinnamon twig 9 parts, motherwort 13 parts, chuanxiong rhizome 14 parts, prepared licorice root 14 parts.

[0079] Prescription 2: White peony root 28 parts, cinnamon twig 9 parts, motherwort 9 parts, chuanxiong rhizome 14 parts, cyperus rhizome 7 parts, chicken dung vine 8 parts, prepared licorice root 9 parts.

[0080] Prescription 3: 40 parts white peony root, 13 parts cinnamon twig, 13 parts peach kernel, 20 parts chuanxiong rhizome, 13 parts immature bitter orange, 13 parts poria cocos, and 13 parts prepared licorice root.

[0081] Positive drug group: Yimu Sheng Hua San

[0082] Control group: No treatment received.

[0083] (II) Test Methods

[0084] Eighty-eight newly calved dairy cows were selected and divided into five groups: a control group (n=18), a positive drug group (n=18), and prescription group 1 (n=17), prescription group 2 (n=18), and prescription group 3 (n=17). Cows in the positive drug group, prescription group 1, prescription group 2, and prescription group 3 received 755g of each prescription administered orally daily for three consecutive days postpartum. The control group received no treatment. The incidence of peripartum diseases in each group was then statistically analyzed to observe the effects of traditional Chinese veterinary medicine on the incidence of peripartum diseases, milk production performance, milk quality, uterine involution score, and feeding time in dairy cows.

[0085] (III) Test Results

[0086] Table 3 shows the disease incidence rates in dairy cows during the peripartum period (21 days postpartum). Regarding the incidence of metritis, prescription group 3 showed the largest decrease, with a reduction of 21.57 percentage points (11.76% vs 33.33%) compared to the control group. In terms of overall incidence, the control group had an incidence of 44.44%, the positive control group 33.33%, prescription group 1 29.41%, prescription group 2 33.33%, and prescription group 3 17.65%. Prescription group 3 showed the largest decrease, with a reduction of 26.79 percentage points (17.65% vs 44.44%) compared to the control group. These results indicate that prescription 3 can reduce the incidence of postpartum metritis and the overall disease incidence rate in dairy cows, thus improving their postpartum health.

[0087] Results of milk production performance in dairy cows 30 days postpartum: Figure 5 As shown, milk production steadily increased in all groups postpartum. Prescription 3 showed the highest milk production, and its milk production on postpartum days 6-9, 12, and 20-30 (d6-9, d12, d20-30) was significantly higher than that of the control group, with statistically significant differences. P The value was <0.05), and the growth trend was stable. These results indicate that Prescription 3 can significantly improve the postpartum milk production performance of dairy cows.

[0088] DHI milk quality test results in the first month postpartum: Figure 6 As shown. Regarding milk fat content, prescription three had the highest milk fat content, and there was a statistically significant difference compared to prescriptions one and two. P <0.05); Regarding milk protein content, prescription three had the highest milk protein content, and there was a statistically significant difference compared to prescription one, prescription two, the positive drug group, and the blank control group. P <0.05); there was no significant difference in lactose content among the groups; at the somatic cell level, prescription three had the lowest level, but there was no statistically significant difference ( P >0.05); the above results indicate that prescription three can increase the content of milk fat and milk protein in dairy cows, reduce the somatic cell count level, and improve the quality of dairy cow milk.

[0089] The results of blood ketone testing in dairy cows on the 14th day postpartum are as follows: Figure 7 As shown, the overall blood ketone level of prescription three was at its lowest, and the blood ketone levels of each group remained within the normal physiological range (BHBA < 1.2 mmol / L), indicating that prescription three has a tendency to improve the negative energy balance of dairy cows after calving, improve the postpartum health of dairy cows, and will not affect the energy metabolism problem of dairy cows after calving.

[0090] The results of the uterine involution score of dairy cows 21 days postpartum are as follows: Figure 8 As shown. The scores of prescription group three were lower than other groups at postpartum days 7, 14, and 21. Specifically, there were statistically significant differences between prescription group three and the control group at postpartum days 7 and 14. P<0.05), indicating that prescription three has a significant effect on promoting uterine involution in dairy cows after calving.

[0091] Results of dairy cow feeding time from 7 days before calving to 21 days after calving: Figure 9 As shown, the feeding times of dairy cows in each group were basically the same before and after calving, with no statistically significant differences. P >0.05), but on the day of calving (d0), the feeding time in the three prescription groups was significantly longer, and there was a statistically significant difference compared with the control group. P <0.05), indicating that prescription three can promote the recovery of appetite in dairy cows on the day of calving.

[0092] Table 3. Statistical table of peripartum disease incidence in dairy cows of different groups

[0093]

[0094] Example 4: Preparation of the pharmaceutical composition and feeding trial in dairy cows

[0095] As can be seen from Examples 3 and 2, prescription 3 showed the best effect among the three prescriptions. Furthermore, compared to the control group, prescription 3, administered once or three times postpartum, resulted in a 50% reduction in the incidence of postpartum endometritis (44.77% vs 64.72%) and a 55%–60% reduction in the total incidence of perinatal diseases (55.65% vs 60.28%), with no significant difference. P (>0.05), therefore, through two trials, it was determined that prescription three is currently the most effective, and the cost-effective course of treatment is a single postpartum dose. To further determine whether there is a synergistic effect among the individual herbs in prescription three, the following trials were conducted.

[0096] (a) Investigational drugs

[0097] Control group: Administered equal volume of water orally;

[0098] Positive drug group: Administered Yimu Shenghua San orally;

[0099] Experimental Example 1: A traditional Chinese veterinary medicine composition consisting of 40 parts white peony root, 13 parts cinnamon twig, 13 parts peach kernel, 20 parts chuanxiong rhizome, 13 parts immature bitter orange, 13 parts poria cocos, and 13 parts prepared licorice root;

[0100] Experimental Example 2: A traditional Chinese veterinary medicine composition consisting of 10 parts white peony root, 10 parts cinnamon twig, 10 parts peach kernel, 15 parts chuanxiong rhizome, 10 parts immature bitter orange, 10 parts poria cocos, and 10 parts prepared licorice root;

[0101] Experimental Example 3: A traditional Chinese veterinary medicine composition consisting of 15 parts white peony root, 12 parts cinnamon twig, 12 parts peach kernel, 14 parts chuanxiong rhizome, 9 parts immature bitter orange, 9 parts poria cocos, and 12 parts prepared licorice root;

[0102] Comparative Group 1: A traditional Chinese veterinary medicine composition containing only white peony root;

[0103] Comparative Group 2: A traditional Chinese veterinary medicine composition containing only cinnamon twigs;

[0104] Comparative Group 3: A traditional Chinese veterinary medicine composition containing only peach kernels;

[0105] Comparative group 4: Traditional Chinese veterinary medicine composition containing only Ligusticum chuanxiong;

[0106] Comparative Group 5: A traditional Chinese veterinary medicine composition containing only Citrus aurantium;

[0107] Comparative Group 6: A traditional Chinese veterinary medicine composition containing only Poria cocos;

[0108] Comparative Group 7: A traditional Chinese veterinary medicine composition containing only roasted licorice root;

[0109] Experimental Methods: 180 newly calved dairy cows were selected and divided into 12 groups (n=15): a blank control group, a positive drug group, experimental case 1, experimental case 2, experimental case 3, and control group 1, control group 2, control group 3, control group 4, control group 5, control group 6, and control group 7. Each drug group received 755g of each prescription diluted in 8L of water at approximately 35-40℃ after calving, stirred well, and administered once. The blank control group received 8L of water at approximately 35-40℃ without the drug. The effects of traditional Chinese veterinary drugs on the incidence of peripartum diseases and milk production performance in dairy cows were observed.

[0110] (iv) Experimental Results

[0111] Table 4 shows the incidence rates of peripartum diseases (21 days postpartum) in dairy cows, including metritis, retained placenta, mastitis, abomasal displacement, and digestive disorders. Regarding the incidence of metritis, the incidence rate in Experimental Case 1 was 13.33%, lower than that in the positive drug group and all control groups. In terms of overall incidence, Experimental Cases 1-3 were all lower than those in Control Groups 1-7. These results indicate that the experimental groups effectively reduced the overall incidence of postpartum diseases in dairy cows. The seven drugs in the experimental groups showed a synergistic effect in reducing postpartum morbidity and effectively improved the postpartum health of dairy cows.

[0112] Results of milk production performance in dairy cows 30 days postpartum: Figure 10 As shown in the figure, milk production in all groups showed an increasing trend at 30 days postpartum, indicating that each group had a certain effect on improving the milk production performance of dairy cows. Group 1 maintained the highest level and was significantly higher than the blank control group on day 22 (d22), showing a statistically significant difference. P <0.05). The milk production in Experiment 1 was higher than that in Control Groups 1-7 from day 6 to 30 postpartum, indicating that the seven herbs in Experiment 1 had a synergistic effect in increasing milk production.

[0113] Blood ketone test results on the 14th day postpartum: Figure 11 As shown, the blood ketone levels in each group remained within the normal physiological range (BHBA < 1.2 mmol / L), indicating that the treatments did not affect the postpartum energy metabolism of dairy cows.

[0114] Table 4. Statistical table of peripartum disease incidence in dairy cows of different groups

[0115]

[0116] The embodiments described above are only some embodiments of the present invention, not all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are all within the scope of protection of the present invention.

Claims

1. A traditional Chinese medicine composition for improving the postpartum health of dairy cows, characterized in that, The composition is made from white peony root, cinnamon twig, peach kernel, chuanxiong rhizome, immature bitter orange, poria cocos, and prepared licorice root, wherein the mass ratio of white peony root, cinnamon twig, peach kernel, chuanxiong rhizome, immature bitter orange, poria cocos, and prepared licorice root is 10-40:10-13:10-13:14-20:9-13:9-13:10-13.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, The mass ratio of the following ingredients is 40:13:13:20:13:13:

13.

3. The traditional Chinese medicine composition according to claim 1 or 2, characterized in that, The preparation method of the traditional Chinese medicine composition includes the steps of raw material crushing, sieving, mixing and packaging.

4. The application of the traditional Chinese medicine composition according to claim 1 or 2 in the preparation of products that can improve the postpartum health of dairy cows, characterized in that, The product is either animal feed or medicine.

5. The application according to claim 4, characterized in that, The application is in the preparation of products that improve postpartum feed intake, rumination time, and / or feeding duration in dairy cows.

6. The application according to claim 4, characterized in that, The application is in the preparation of products that increase the amount of exercise in dairy cows after calving.

7. The application according to claim 4, characterized in that, The application is in the preparation of products that improve the milk yield and / or milk quality of dairy cows after calving.

8. The application according to claim 7, characterized in that, The quality of the milk produced includes the milk protein content and / or milk fat content.

9. The application of the traditional Chinese medicine composition according to claim 1 or 2 in the preparation of products for preventing and treating postpartum diseases in dairy cows, characterized in that, The product in question is a pharmaceutical product.

10. The application according to claim 9, characterized in that, The postpartum conditions mentioned include endometritis, retained placenta, mastitis, abomasal displacement, or digestive disorders.

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