Ginseng polygonum multiflorum cultivation method

Abstract

The invention relates to the technical field of plant cultivation, and particularly discloses a ginseng and polygonum multiflorum cultivation method which comprises the following steps: collecting healthy and well-growing ginseng and polygonum multiflorum biennial tuberous roots, and cleaning and disinfecting the ginseng and polygonum multiflorum biennial tuberous roots; bud points on the surfaces of the tuberous roots are taken and inoculated into a primary induction culture medium for primary induction differentiation culture, and sterile buds are obtained; the sterile buds are trimmed into small bud stem sections with the length of about 0.5-1 cm, and the small lateral bud stem sections are inoculated into a subculture multiplication culture medium for subculture to form plantlets; the proliferation culture medium is an MS culture medium containing 2.0 to 5.0 mg / L of BAP and 0.2 to 0.5 mg / L of NAA; transferring the plantlets into a rooting culture medium for rooting culture to obtain robust plantlets; and domesticating and transplanting the robust seedlings. According to the cultivation method of ginseng and polygonum multiflorum, the ginseng and polygonum multiflorum seedlings are obtained through tissue culture of tuberous root buds, the advantages of being healthy, high in disease resistance, high in survival rate and the like are achieved, components and characteristics of original mother seeds can be completely preserved, and healthy breeding and seedling raising are achieved.

Description

Technical Field

[0001] This invention relates to the field of plant cultivation technology, and in particular to a cultivation method for ginseng and Polygonum multiflorum. Background Technology

[0002] Ginseng He Shou Wu is a perennial twining herb. Its vines are known as "Ye Jiao Teng" in traditional Chinese medicine. It is neutral in nature, sweet and slightly bitter in taste, and has the effects of nourishing the heart and calming the mind, dispelling wind and unblocking the meridians. The underground tuberous root, after processing, is called "Ginseng He Shou Wu." It is bitter and slightly warm in nature, and can tonify the liver and kidneys, replenish essence and blood, strengthen muscles and bones, and darken hair, possessing both nourishing and strengthening effects. Its stems and vines are also commonly used to treat insomnia.

[0003] The tuberous roots of Ginseng Polygonum multiflorum contain active ingredients such as saponins, ginseng polysaccharides, volatile oils, amino acids, inorganic elements, peptides, vitamins, organic acids, alkaloids, flavonoids, and enzymes, especially renowned for their phenolic and glycophenolic compounds. The darker the outer skin of the tuberous root, the stronger its aroma; the longer the cultivation period, the higher its value, hence the nickname "black gold." Ginseng Polygonum multiflorum was first discovered in a high-altitude tribe in Taiwan. After years of research, botanists selected and hybridized wild varieties to cultivate new ginseng Polygonum multiflorum varieties, which are now widely cultivated in Taiwan and have become a local specialty. Current cultivation systems rely heavily on asexual reproduction, cuttings, or rhizome propagation, commonly using commercially discarded inferior tubers or unselected vine cuttings as seed sources. This easily leads to germplasm degradation, resulting in reduced survival rates, decreased tuber biomass, significantly reduced resistance to pests and diseases, and severe disease outbreaks, all of which affect the medicinal quality of Ginseng Polygonum multiflorum. Summary of the Invention

[0004] To address the above shortcomings, this invention provides a cultivation method for ginseng and Polygonum multiflorum. By cultivating ginseng and Polygonum multiflorum seedlings, the seedlings can be deviralized, exhibiting strong disease resistance and a high survival rate. The specific technical solution is as follows: A method for cultivating ginseng and Polygonum multiflorum includes the following steps: (1) Collect healthy and well-grown two-year-old tuberous roots of ginseng and Polygonum multiflorum, and clean and disinfect them; (2) Take the buds on the surface of the tuber and inoculate them into the primary induction medium for primary induction differentiation culture to obtain sterile buds; the primary induction medium is MS medium containing 1.0~3.5 mg / L BAP (6-benzyladenine); (3) Trim the aseptic buds into bud stem segments of about 0.5-1cm in length, and inoculate the lateral bud stem segments into the subculture proliferation medium for subculture to form seedlings; the subculture proliferation medium is MS medium containing 2.0-5.0mg / L BAP and 0.2-0.5mg / L NAA (naphthaleneacetic acid); (4) Transfer the seedlings to the rooting medium for rooting culture to grow into robust seedlings; (5) Remove the healthy seedlings from the bottle and plant them in planting cups filled with potting soil for acclimatization to obtain large seedlings; (6) Transplant the acclimatized seedlings to the field and carry out field management.

[0005] Preferably, in the above-mentioned cultivation method of ginseng and Polygonum multiflorum, the rooting medium is a 1 / 2 MS medium containing 0.05-0.2 mg / L NAA, with a pH of 5-6.

[0006] Preferably, in the above-mentioned cultivation method of ginseng and Polygonum multiflorum, the cultivation conditions in steps (2), (3), and (4) are a temperature of 23±2℃, a light intensity of 2000~3000Lux, and a light duration of 12~14h / d.

[0007] Preferably, in the above-mentioned cultivation method for ginseng and Polygonum multiflorum, the primary induction medium is MS medium containing 1.0~3.5 mg / L BAP, 15~25 g / L glucose, 4~5.5 g / L agar, and 10~20 mg / L grape seed oil, with a pH of 5~6. Adding grape seed oil to the primary induction medium, which contains a large amount of saturated fatty acids, various vitamins, and antioxidants (such as polyphenols), can improve the stress resistance of bud tissue, accelerate cell repair, slow down the degradation of nutrients, and promote the differentiation of bud tissue.

[0008] Preferably, in the above-mentioned cultivation method for ginseng and Polygonum multiflorum, the proliferation medium is MS medium containing 2.0~5.0 mg / L BAP, 0.2~0.5 mg / L NAA, 15~25 g / L glucose, 4~5.5 g / L agar, and 12~25 mg / L grape seed oil. The proliferation medium can promote the rapid differentiation of bud and stem segments and the formation of new buds, thereby promoting the reproduction and growth of ginseng and Polygonum multiflorum.

[0009] Preferably, in the above-mentioned cultivation method for ginseng and Polygonum multiflorum, the rooting medium is a 1 / 2 MS medium containing 0.05-0.2 mg / L NAA, 18-25 g / L glucose, 4-5.5 g / L agar, and 0.3-0.5 mg / L Polygonum multiflorum polysaccharide, with a pH of 5-6. NAA in the rooting medium effectively stimulates root development and promotes rapid root growth, thus laying the foundation for healthy seedling growth. Polygonum multiflorum polysaccharide contains various active ingredients, and when combined with NAA, it not only further promotes root growth but also enhances the seedling's resistance to adverse conditions. This ensures the smooth development of the seedling's root system and provides stable and efficient technical support for subsequent cultivation and large-scale propagation.

[0010] Preferably, in the above-mentioned cultivation method of ginseng and Polygonum multiflorum, in step (1), disinfection is performed as follows: the cleaned tuberous roots are soaked in a 70% ethanol solution for 1-2 minutes and rinsed with sterile water; then the tuberous roots are soaked in a 0.1%-0.2% mercuric chloride solution for 5-10 minutes and rinsed with sterile water. This disinfection process kills bacteria and some fungi present on the surface of the tuberous roots.

[0011] Preferably, in the above-mentioned cultivation method of ginseng and Polygonum multiflorum, in step (5), before removing the seedlings from the bottle, the healthy seedlings are removed from the bottle and moved out of the greenhouse and placed under a shade net or in a semi-shaded place for 1-2 days. Then, they are planted in planting cups filled with potting soil and placed under a shade net or in a semi-shaded place for 3-5 days. Then, the sunlight exposure time is increased, gradually progressing from 2 hours of sunlight to full sunlight within 5-7 days. The seedlings are acclimatized, with sunlight as the primary focus. The acclimatization is carried out in three stages: weak, medium, and normal. The whole process should be gradual to avoid sudden changes, enhance the plant's resistance to adverse conditions, reduce the stress response caused by environmental changes, and improve the survival rate of transplanted seedlings.

[0012] Preferably, in the above-mentioned cultivation method for ginseng and Polygonum multiflorum, the culture soil, by weight, comprises the following raw materials: 20-30 parts volcanic rock fragments, 12-18 parts akadama soil, 10-15 parts decomposed pine needle soil, 2-4 parts hydroxyapatite, and 1-3 parts seaweed oligosaccharides. The volcanic rock fragments and akadama soil in the culture soil not only provide mineral elements but also improve the microenvironment around the roots. The decomposed pine needle soil contains various active ingredients such as humus, amino acids, and organic matter. Nano-hydroxyapatite slowly releases calcium and phosphorus elements. Combined with seaweed oligosaccharides, the various components interact to promote root development, seedling growth, improve seedling resistance, and increase seedling survival rate.

[0013] Preferably, in the above-mentioned cultivation method of ginseng and Polygonum multiflorum, the raw materials are mixed and then water is added to obtain nutrient soil, and the moisture content of the nutrient soil is 60-70%.

[0014] Compared with the prior art, the beneficial effects of the present invention are: 1. In the cultivation method of ginseng Polygonum multiflorum of the present invention, ginseng Polygonum multiflorum seedlings are obtained by tissue culture using tuberous root buds. These seedlings have the advantages of being healthy, disease-resistant, and having a high survival rate. They can completely preserve the original mother plant components and characteristics, and achieve healthy propagation and seedling cultivation.

[0015] 2. The cultivation method of ginseng and Polygonum multiflorum of the present invention includes a primary induction culture medium, a subculture proliferation culture medium, and a rooting culture medium, which are used for the rapid tissue culture propagation of ginseng and Polygonum multiflorum. It can quickly cultivate a large number of robust ginseng and Polygonum multiflorum seedlings. By optimizing the composition of the culture medium, it promotes the differentiation and proliferation of buds, the growth of seedlings and the development of roots, and improves the stress resistance of seedlings. It is beneficial to improve the survival rate of seedlings after transplanting to the field. It also promotes the growth of roots and tubers after transplanting, thereby achieving the goal of increasing the yield of Polygonum multiflorum. Attached Figure Description

[0016] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 These are robust seedlings obtained through tissue culture in Example 1 of this invention; Figure 2 The seedlings obtained after domestication in Example 1 of this invention are large seedlings. Detailed Implementation

[0018] The specific embodiments of the present invention are described in detail below, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments. Unless otherwise defined, all technical terms used below have the same meaning as commonly understood by those skilled in the art. The technical terms used herein are for the purpose of describing specific embodiments only and are not intended to limit the scope of protection of the present invention. Unless otherwise specifically stated, all raw materials, reagents, instruments, and equipment used in the present invention are commercially available or can be prepared by existing methods.

[0019] Example 1 A method for cultivating ginseng and Polygonum multiflorum includes the following steps: (1) Collect healthy and well-grown two-year-old tuberous roots of ginseng and Polygonum multiflorum, and clean and disinfect them; specifically including: S1. Select two-year-old ginseng and Polygonum multiflorum tubers. These tubers should have sufficient growth potential and be free from pests and diseases to ensure stable subsequent cultivation results. S2. After harvesting, clean the soil and debris off the surface of the tubers. To avoid microorganisms or pathogens that may be attached to the surface of the tubers, gently brush the surface of the tubers with a brush and rinse them thoroughly with running tap water. S3. After washing, wipe the ginseng and Polygonum multiflorum tuber dry with a sterile towel or clean gauze to reduce the interference of moisture on the disinfection process. Soak the tuber in 70% ethanol for 2 minutes to kill any bacteria and some fungi that may be present on the surface. After ethanol treatment, the tuber should be quickly transferred to sterile water to rinse and remove any ethanol residue to avoid adverse effects on subsequent culture. S4. For more thorough disinfection, use a mercuric chloride solution to soak the ginseng and Polygonum multiflorum tuberous roots in a 0.1% mercuric chloride solution for 8 minutes. After soaking, immediately transfer the tubers to sterile water and rinse repeatedly to thoroughly remove any mercuric chloride residue and prevent it from harming the plant tissue. Once the disinfection process is complete, the tissue can be cut. (2) Carefully cut off the healthy buds on the surface of the tuber and inoculate them into the primary induction medium for primary induction differentiation culture. Culture for 25-35 days to obtain sterile buds; the primary induction medium is MS medium containing 2.0 mg / L BAP, 20 g / L glucose and 5 g / L agar; the culture conditions are temperature 23±2℃, light intensity 2000~3000 Lux and light time 12h / d; (3) Trim the aseptic buds into bud stem segments about 1 cm long, and inoculate the lateral bud stem segments into the subculture proliferation medium for subculture for 25-35 days to form seedlings; the subculture proliferation medium is MS medium containing 2.0 mg / L BAP, 0.3 mg / L NAA, 20 g / L glucose and 5 g / L agar; culture conditions: light time 12 h / d, light intensity 2000-3000 lx, culture temperature 23 ± 2°C; (4) Transfer the seedlings to the rooting medium for rooting culture to grow into robust seedlings (see Figure 1 The rooting medium was a 1 / 2 MS medium containing 0.15 mg / L NAA, 20 g / L glucose, and 5 g / L agar, with a pH of 5.7. The medium was sterilized at 121°C for 20 minutes. The culture conditions were: 12 h / d light exposure, 2000-3000 lx light intensity, and 23 ± 2°C. (5) Move the bottles containing healthy seedlings from the greenhouse to the outdoors, where the outdoor temperature is 20-25℃. Place them under a shade net or in a semi-shaded area for one day, and then transplant them into planting cups containing potting soil (see See Planting Cups). Figure 2 Place the seedlings under a shade net or in partial shade for 3 days. Then, starting from the 4th day, expose them to sunlight through the shade net, with 2 hours of sunlight exposure on the 4th day, increasing by 2 hours each day until they are exposed to full natural sunlight on the 8th day. Then, cultivate them in a natural environment for 36-40 days to obtain acclimatized seedlings (see...). Figure 2 ); The potting soil contains the following raw materials: 25 parts volcanic rock fragments, 15 parts Akadama soil, 12 parts decomposed pine needle soil, 3 parts hydroxyapatite, and 2 parts seaweed oligosaccharides. The above raw materials are mixed with water to obtain potting soil with a moisture content of 60-70%.

[0020] (6) Transplant the acclimatized seedlings to the field and carry out field management.

[0021] Example 2 A method for cultivating ginseng and Polygonum multiflorum includes the following steps: (1) Collect healthy and well-grown two-year-old tuberous roots of ginseng and Polygonum multiflorum, and clean and disinfect them; specifically including: S1. Select two-year-old ginseng and Polygonum multiflorum tubers. These tubers should have sufficient growth potential and be free from pests and diseases to ensure stable subsequent cultivation results. S2. After harvesting, clean the soil and debris off the surface of the tubers. To avoid microorganisms or pathogens that may be attached to the surface of the tubers, gently brush the surface of the tubers with a brush and rinse them thoroughly with running tap water. S3. After washing, wipe the ginseng and Polygonum multiflorum tuber dry with a sterile towel or clean gauze to reduce the interference of moisture on the disinfection process. Soak the tuber in 70% ethanol for 2 minutes to kill any bacteria and some fungi that may be present on the surface. After ethanol treatment, the tuber should be quickly transferred to sterile water to rinse and remove any ethanol residue to avoid adverse effects on subsequent culture. S4. For more thorough disinfection, use a mercuric chloride solution to soak the ginseng and Polygonum multiflorum tuberous roots in a 0.1% mercuric chloride solution for 8 minutes. After soaking, immediately transfer the tubers to sterile water and rinse repeatedly to completely remove any mercuric chloride residue and prevent it from harming the plant tissue. Once the disinfection process is complete, the tissue can be cut.

[0022] (2) Carefully cut off the healthy buds on the surface of the tuber and inoculate them into the primary induction medium for primary induction differentiation culture. Culture for 25-35 days to obtain sterile buds. The primary induction medium is MS medium containing 2.0 mg / L BAP, 20 g / L glucose, 5 g / L agar and 15 mg / L grape seed oil. The culture conditions are temperature 23±2℃, light intensity 2000~3000 Lux and light time 12h / d. (3) Cut the aseptic buds into bud stem segments about 1 cm long, and inoculate the lateral bud stem segments into the subculture medium for subculture. Culture for 25-35 days to form seedlings. The subculture medium is MS medium containing 2.0 mg / L BAP, 0.3 mg / L NAA, 20 g / L glucose, 5 g / L agar, and 18 mg / L grape seed oil. Culture conditions: light time 12 h / d, light intensity 2000-3000 lx, and culture temperature 23 ± 2°C. (4) The seedlings were transferred to the rooting medium for rooting culture to grow into robust seedlings; the rooting medium was a 1 / 2 MS medium containing 0.15 mg / L NAA, 20 g / L glucose, 5 g / L agar and 0.4 mg / L Polygonum multiflorum polysaccharide, pH 5.7, and the medium was sterilized at 121°C for 20 minutes; the culture conditions were: light time 12 h / d, light intensity 2000~3000 lx, and culture temperature 23 ± 2°C; (5) Move the bottles containing healthy seedlings from the greenhouse to the outdoors, where the outdoor temperature is 20-25℃. Place them under a shade net or in a semi-shaded area for 1 day. Then, remove them from the bottles and plant them in planting cups containing potting soil. Place them under a shade net or in a semi-shaded area for 3 days. Then, starting from the 4th day, expose them to sunlight through the shade net. The sunlight exposure time on the 4th day is 2 hours, increasing by 2 hours each day. On the 8th day, the seedlings are gradually exposed to full natural sunlight. Then, cultivate them in a natural environment for 36-40 days to obtain acclimatized large seedlings. The potting soil contains the following raw materials: 25 parts volcanic rock fragments, 15 parts Akadama soil, 12 parts decomposed pine needle soil, 3 parts hydroxyapatite, and 2 parts seaweed oligosaccharides. The above raw materials are mixed with water to obtain potting soil with a moisture content of 60-70%.

[0023] (6) Transplant the acclimatized seedlings to the field and carry out field management.

[0024] Comparative Example 1 The difference between this comparative example and Example 1 is that 1 mg / L KT (furanoaminopurine) was added to the primary induction medium, while the other components were the same as in Example 1.

[0025] Comparative Example 2 The difference between this comparative example and Example 2 is that the grape seed oil content in the primary induction medium is 30 mg / L, while the other components are the same as in Example 2.

[0026] Comparative Example 3 The difference between this comparative example and Example 2 is that the grape seed oil content in the primary induction medium is 5 mg / L, while the other components are the same as in Example 2.

[0027] Comparative Example 4 The difference between this comparative example and Example 2 is that the grape seed oil content in the subculture proliferation medium is 30 mg / L, while the other components are the same as in Example 2.

[0028] Comparative Example 5 The difference between this comparative example and Example 2 is that the Polygonum multiflorum polysaccharide content in the rooting medium is 1 mg / L.

[0029] Experimental Example 1 Example 1, Example 2, and Comparative Examples 1, 2, and 3 were set up, and primary induction differentiation culture was performed according to the corresponding methods. Each group had 40 buds, and the culture was carried out for 30 days. The induction rate and number of buds in each group were recorded. The results are shown in Table 1. Table 1 shows that adding a certain amount of grape seed oil can improve the induction rate and bud height; however, excessively high concentrations of grape seed oil can have an inhibitory effect. The addition of KT also has a certain inhibitory effect on bud formation.

[0030] Table 1 Differentiation induction status of each group

[0031] Experimental Example 2 Group 1, Group 2, and Comparative Group 4 were set up. Following the implementation method, aseptic buds were trimmed into 1cm long bud-stem segments. These lateral bud-stem segments were inoculated into the corresponding subculture proliferation medium for subculture. Forty buds were inoculated into each treatment. After 30 days, the proliferation rate and average number of buds were calculated. Proliferation rate (%) = (number of buds / total number of inoculated buds) × 100; average number of buds = (total number of buds / number of buds). The results are shown in Table 2.

[0032] Table 2. Aseptic bud proliferation in each group

[0033] Experimental Example 3 Five groups were set up: Example 1, Example 2, and Comparative Example 5. Following the implementation method, seedlings were transferred to the corresponding rooting medium for rooting culture. Each treatment had 30 seedlings inoculated. After 30 days, the rooting rate and average number of roots were calculated. Rooting rate (%) = number of roots / total number of inoculated seedlings × 100. The results are shown in Table 3.

[0034] As shown in Table 3, adding an appropriate amount of Polygonum multiflorum polysaccharide to the rooting medium can promote the rooting of seedlings.

[0035] Table 3 Rooting status of each group

[0036] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A method for cultivating ginseng and Polygonum multiflorum, characterized in that, Includes the following steps: (1) Collect healthy and well-grown two-year-old tuberous roots of ginseng and Polygonum multiflorum, and clean and disinfect them; (2) Take the buds on the surface of the tuber and inoculate them into the primary induction medium for primary induction differentiation culture to obtain sterile buds; the primary induction medium is MS medium containing 1.0~3.5 mg / L BAP; (3) Trim the aseptic buds into bud stem segments of about 0.5-1cm in length, and inoculate the lateral bud stem segments into the subculture proliferation medium for subculture to form seedlings; the proliferation medium is MS medium containing 2.0-5.0mg / L BAP and 0.2-0.5mg / L NAA; (4) Transfer the seedlings to the rooting medium for rooting culture to grow into robust seedlings; (5) Remove the healthy seedlings from the bottle and plant them in planting cups filled with potting soil for acclimatization to obtain large seedlings; (6) Transplant the acclimatized seedlings to the field and carry out field management.

2. The cultivation method of ginseng and Polygonum multiflorum according to claim 1, characterized in that, The rooting medium is a 1 / 2 MS medium containing 0.05-0.2 mg / L NAA.

3. The cultivation method of ginseng and Polygonum multiflorum according to claim 1, characterized in that, In steps (2), (3), and (4), the cultivation conditions are a temperature of 23±2℃, a light intensity of 2000~3000Lux, and a light exposure time of 12~14h / d.

4. The cultivation method of ginseng and Polygonum multiflorum according to claim 1, characterized in that, The primary induction medium was MS medium containing 1.0–3.5 mg / L BAP, 15–25 g / L glucose, 4–5.5 g / L agar, and 10–20 mg / L grape seed oil.

5. The cultivation method of ginseng and Polygonum multiflorum according to claim 1, characterized in that, The proliferation medium is MS medium containing 2.0~5.0 mg / L BAP, 0.2~0.5 mg / L NAA, 15~25 g / L glucose, 4~5.5 g / L agar, and 12~25 mg / L grape seed oil.

6. The cultivation method of ginseng and Polygonum multiflorum according to claim 1, characterized in that, The rooting medium is a 1 / 2 MS medium containing 0.05-0.2 mg / L NAA, 18-25 g / L glucose, 4-5.5 g / L agar, and 0.3-0.5 mg / L Polygonum multiflorum polysaccharide, with a pH of 5-6.

7. The cultivation method of ginseng and Polygonum multiflorum according to claim 1, characterized in that, In step (1), disinfection is performed by soaking the cleaned tubers in a 70% ethanol solution for 1-2 minutes and rinsing them with sterile water; then soaking the tubers in a mercuric chloride solution with a mass percentage concentration of 0.1%-0.2% for 5-10 minutes and rinsing them with sterile water.

8. The cultivation method of ginseng and Polygonum multiflorum according to claim 1, characterized in that, In step (5), before removing the seedlings from the bottle, the healthy seedlings are removed from the bottle and moved out of the greenhouse. They are placed under a shade net or in a semi-shaded place for 1-2 days. Then, they are planted in planting cups filled with potting soil and placed under a shade net or in a semi-shaded place for 3-5 days. Then, the sunlight exposure time is increased, gradually progressing from 2 hours of sunlight to full sunlight environment within 5-7 days.

9. The cultivation method of ginseng and Polygonum multiflorum according to claim 8, characterized in that, By weight, the culture medium contains the following raw materials: 20-30 parts volcanic rock fragments, 12-18 parts Akadama soil, 10-15 parts decomposed pine needle soil, 2-4 parts hydroxyapatite, and 1-3 parts seaweed oligosaccharides.

10. The cultivation method of ginseng and Polygonum multiflorum according to claim 9, characterized in that, The raw materials are mixed, and then water is added to obtain nutrient soil. The moisture content of the nutrient soil is 60-70%.

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