Nanoparticle separation method and device based on electric field and flow field driving

Abstract

The invention provides a nano-particle separation method and device based on electric field and flow field driving. The method comprises the steps of mounting a separation membrane and configuring flow field parameters and electric field parameters of the nano-particle separation device. The method comprises the following steps: loading a sample to be measured into a nanoparticle separation device, starting the nanoparticle separation device, and providing a flow field and an electric field for the sample to be measured so as to separate nanoparticles in the sample to be measured. And monitoring the parameter variation of the electrophoretic separation device, adjusting the flow field parameter and the electric field parameter of the electrophoretic separation device, and recovering the sample to be measured from the half chambers on the two sides of the electrophoretic separation device. The high-precision shaking table shakes to generate local high-shear flow on the surface of the separation membrane and prevent particles from depositing on the surface of the separation membrane. And applying an electric field to drive the charged nanoparticles to perform electrophoretic motion, so that the particles are driven to or away from the surface of the separation membrane, and the particles with specific charges are pre-enriched or repelled, so that the transmembrane flux of the target particles is improved, and meanwhile, the membrane adsorption of pollutants is reduced.

Description

Technical Field

[0001] This invention relates to the field of nanoparticle separation and separation device technology, specifically to a nanoparticle separation method and device driven by electric field and flow field. Background Technology

[0002] Soil colloids and nanoparticles are the smallest (particle size between 1–1000 nm) and most active components constituting the soil solid-phase framework. Key processes in soil, such as adsorption-desorption, complexation-transformation, migration-fate of heavy metals, persistent organic pollutants, and nutrients, are closely related to the occurrence state, surface properties, and particle size distribution of colloids and nanoparticles. For example, smaller colloids (< 100 nm) often exhibit higher migration capacity and pollutant loading capacity due to stronger Brownian motion and larger specific surface area. Larger colloids (> 200 nm) are more easily trapped in porous media, becoming sites of pollutant aggregation. Therefore, achieving precise separation and systematic characterization of different particle size components in soil colloids is not only the scientific basis for revealing their environmental interfacial behavior mechanisms but also a key technological prerequisite for accurately assessing the bioavailability of pollutants, predicting the long-term environmental risks of pollutants, and developing targeted environmental remediation strategies.

[0003] Currently, the separation and characterization of soil colloids and nanoparticles of different particle sizes still mainly rely on traditional physical methods such as centrifugation and filtration. Patent CN101776545A discloses a method for separating and purifying soil colloids based on high-speed centrifugation (8000-12000 rpm). This method obtains the target colloid through repeated centrifugation and decantation, but it suffers from problems such as low separation resolution, complex sample pretreatment, easy particle aggregation or loss, and difficulty in achieving high-throughput parallel processing. Especially for experiments requiring simultaneous study of multiple particle size ranges or different adsorption conditions, traditional methods are inefficient and cannot meet the needs of systematic research. Meanwhile, new technologies such as microfluidics and ultrafiltration have been introduced into the field of nanoparticle separation, improving separation accuracy and gentleness. However, when applied to complex environmental samples such as soil colloids, they still face severe challenges such as low throughput, poor universality, and high operational specialization requirements.

[0004] Furthermore, the separation processes of centrifugation, filtration, and microfluidics primarily rely on a single physical field (such as a centrifugal force field or a pressure field). For complex nanoparticle systems with varying surface charges and significant differences between their hydration radii and hydrodynamic radii (such as protein aggregates, surface-modified engineered nanoparticles, and natural organic-inorganic composite colloids), the separation selectivity of a single physical field is limited, making it difficult to achieve fine sorting based on multiple dimensions such as size, charge, and surface properties. In particular, membrane fouling and concentration polarization, common problems in traditional dynamic filtration, restrict separation flux and stability. Therefore, there is an urgent need for a nanoparticle separation method and device that can couple multiple physical fields (such as electric fields and flow fields) and possess high throughput, high selectivity, and anti-fouling properties. Summary of the Invention

[0005] To overcome the problems existing in related technologies, the purpose of this invention is to provide a nanoparticle separation method and device based on electric field and flow field driven, which can couple the flow field and electric field, and has the advantages of high throughput, high selectivity and anti-pollution.

[0006] A method and apparatus for separating nanoparticles driven by electric and flow fields, comprising: Install the separation membrane and configure the flow field and electric field parameters of the nanoparticle separation device; The sample to be measured is loaded into the nanoparticle separation device, and the nanoparticle separation device is started to provide a flow field and an electric field to the sample to be measured in order to separate the nanoparticles in the sample. Monitor the parameter changes of the electrophoretic separation device, and adjust the flow field parameters and electric field parameters of the electrophoretic separation device accordingly; The sample to be measured is recovered from the half-chambers on both sides of the electrophoretic separation device.

[0007] In a preferred embodiment of the present invention, loading the sample to be measured into the nanoparticle separation device includes: Inject a suspension containing nanoparticles into the original chamber; The receiving liquid or blank solution is injected into the receiving chamber; the original chamber and the receiving chamber are located on opposite sides of the electrophoretic separation device.

[0008] In a preferred embodiment of the present invention, the suspension further includes a liquid medium and a dispersant compatible with the nanoparticles; the liquid medium is deionized water or a buffer solution, and the dispersant is sodium hexametaphosphate or a surfactant.

[0009] In a preferred embodiment of the present invention, the step of activating the nanoparticle separation device to provide a flow field and an electric field to the sample to be measured includes: Start the shaking table and electrophoretic separation device; Control the shaking of the shaker to generate shear force or turbulence at the interface between the separation membrane and the suspension; The electrophoretic separation device is controlled to generate an alternating electric field or pulse to drive the target particles to migrate toward the separation membrane.

[0010] In a preferred embodiment of the present invention, the flow field parameters include rotational speed and oscillation mode, and the electric field parameters include DC bias voltage and AC frequency.

[0011] In a preferred embodiment of the present invention, the nanoparticles are any one of environmental colloidal particles, engineering and material nanoparticles, bio-derived nanoparticles, and pollutant carrier particles.

[0012] A nanoparticle separation device based on electric field and flow field driven method is provided. The device includes a batch adsorption device with a shaking table connected to its bottom and an electrophoretic separation device electrically connected to it.

[0013] In a preferred embodiment of the present invention, the batch adsorption device includes two opposing drive frames, a support frame is provided between the two drive frames, and an adsorption unit is provided inside the support frame.

[0014] In a preferred embodiment of the present invention, the adsorption unit includes a first fixing plate, a second fixing plate, and a separation membrane. A primary chamber is provided between the first fixing plate and the separation membrane, and a receiving chamber is provided between the second fixing plate and the separation membrane. Both the first fixing plate and the second fixing plate are annular structures.

[0015] In a preferred embodiment of the present invention, the nanoparticle separation device driven by electric field and flow field further includes a gasket, a positive electrode is installed in the original chamber near the receiving chamber, and a negative electrode is installed in the receiving chamber near the original chamber; the separation membrane is adapted to the gasket, the separation membrane is installed in the gasket, and the gasket has a ring structure.

[0016] The beneficial effects of this invention are as follows: This invention provides a nanoparticle separation method based on electric and flow field driven processes, including installing a separation membrane and configuring the flow field and electric field parameters of the nanoparticle separation device. The sample to be measured is loaded into the nanoparticle separation device, which is then activated to provide flow and electric fields to the sample, thereby separating the nanoparticles. The parameters of the electrophoretic separation device are monitored, and the flow and electric field parameters are adjusted accordingly. The sample is then recovered from the two half-chambers on either side of the electrophoretic separation device. The flow field is provided through shaking and rotation. The inner cavity of the original chamber is designed with a shallow dish-shaped guide surface, allowing the sample liquid to form a uniform liquid layer during rotation, thus covering the entire separation membrane surface. A high-precision shaker generates shaking, which creates localized high-shear flow on the separation membrane surface, effectively disturbing the boundary layer, preventing particle deposition on the separation membrane surface, mitigating concentration polarization and membrane fouling, and maintaining long-term stability of the separation flux. Applying an electric field to drive charged nanoparticles to undergo electrophoretic motion can actively drive particles toward or away from the separation membrane surface, achieving pre-enrichment or repulsion of particles with specific charges, thereby increasing the transmembrane flux of target particles while reducing the membrane adsorption of pollutants. Attached Figure Description

[0017] Figure 1 This is a flowchart of the nanoparticle separation method based on electric field and flow field driven by the present invention; Figure 2 This is a structural diagram of the nanoparticle separation device based on electric field and flow field driven by the present invention; Figure 3 This is a composition diagram of the adsorption unit of the present invention.

[0018] Reference numerals: 1. Batch adsorption device; 2. Shaker; 3. Electrophoretic separation device; 4. Drive frame; 5. Support frame; 6. Adsorption unit; 7. First fixing plate; 8. Primary chamber; 9. Receiving chamber; 10. Positive electrode; 11. Negative electrode; 12. Separation membrane; 13. Gasket; 14. Fixing screw; 15. Sealing plug; 16. Second fixing plate. Detailed Implementation

[0019] Preferred embodiments of the invention will now be described in more detail with reference to the accompanying drawings. While preferred embodiments of the invention are shown in the drawings, it should be understood that the invention can be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that the invention will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

[0020] Example 1 like Figure 1 As shown, this embodiment provides a nanoparticle separation method based on electric field and flow field driven by the following: S1: Install the separation membrane and configure the flow field parameters and electric field parameters of the nanoparticle separation device.

[0021] S2: Load the sample to be measured into the nanoparticle separation device, start the nanoparticle separation device, and provide a flow field and an electric field to the sample to be measured in order to separate the nanoparticles in the sample.

[0022] S3: Monitor the parameter changes of the electrophoretic separation device and adjust the flow field parameters and electric field parameters of the electrophoretic separation device.

[0023] S4: Recover the sample to be measured from the half-chambers on both sides of the electrophoretic separation device.

[0024] Based on the properties of the target particles, a pore size corresponding to the target particles is selected, and the separation membrane 12 is determined according to the pore size. The pore size range of the separation membrane 12 is 1-1000 nm. The particle size of the target particles is positively correlated with the pore size of the separation membrane 12, that is, the smaller the particle size of the target particles, the smaller the pore size of the separation membrane 12.

[0025] The step of loading the sample to be measured into the nanoparticle separation device includes: S21: Inject the suspension containing nanoparticles into the original chamber; S22: Inject the receiving liquid or blank solution into the receiving chamber; the original chamber and the receiving chamber are located on both sides of the electrophoretic separation device.

[0026] The nanoparticle separation device consists of two half-chambers, each made of chemically inert materials such as Teflon. The half-chambers are flat cylindrical in shape, and each half-chamber has a volume of 10 mL.

[0027] During operation, the sample suspension is injected into the primary chamber 8, and the receiving liquid is injected into the receiving chamber 9. Electrode interfaces are provided on the half-chamber walls of the primary chamber 8 and the receiving chamber 9. The electrode interfaces are connected to the electrophoretic separation device 3, which is used to generate an electric field between the primary chamber 8 and the receiving chamber 9, so that the charged nanoparticles in the sample to be measured move from the primary chamber 8 to the receiving chamber 9.

[0028] The suspension consists of nanoparticles to be separated and a liquid medium, typically compatible with both the particles and the membrane material, such as deionized water or a buffer solution with a specific pH. To prevent particle aggregation, a trace amount of dispersant, such as sodium hexametaphosphate or a surfactant, can be added. The receiving liquid must be chemically miscible and compatible with the dispersion medium of the suspension; typically, the same background solution (without nanoparticles) as the suspension is used directly. This maintains chemical equilibrium across the membrane, with parameters including osmotic pressure, ionic strength, and pH, preventing abnormal water flux or particle behavior caused by chemical gradients. The selected separation membrane 12 is placed between the two half-chambers, and the original chamber 8 and the receiving chamber 9 are sealed with a sealing plug 15 to form an independent adsorption unit 6. Multiple adsorption units 6 are installed in the mounting positions of the support frame 5. The flow field and electric field co-drive system is activated to begin the separation of nanoparticles.

[0029] The activation of the nanoparticle separation device provides a flow field and an electric field to the sample to be measured, including: S23: Start the shaking table and electrophoretic separation device; S24: Control the shaking of the shaker to generate shear force or turbulence at the interface between the separation membrane and the suspension; S25: Control the electrophoretic separation device to generate an alternating electric field or pulse to drive the target particles to migrate toward the separation membrane.

[0030] The shaking table 2 provides the horizontal fluid driving force, while the electrophoretic separation device 3 provides the electric field force. The high-precision shaking table 2 has an amplitude of 0.1-5 mm and a frequency of 0.1-10 Hz. The main function of the shaking table 2 is to generate local high-shear flow on the membrane surface, effectively disturbing the boundary layer, preventing particle deposition on the membrane surface, alleviating concentration polarization and membrane fouling, and maintaining long-term stability of the separation flux.

[0031] Electrophoretic separation device 3 applies a DC electric field, utilizing electrophoresis to directionally migrate particles. Alternatively, electrophoretic separation device 3 applies an AC / pulsed electric field, utilizing dielectric electrophoresis to generate selective forces on particles of different sizes / dielectric constants, or by periodically reverse electroosmotic flow to scour the membrane surface. Dielectric electrophoresis is a physical phenomenon in which neutral particles undergo directional motion due to dielectric polarization in a non-uniform AC electric field.

[0032] By programming and controlling the combination of parameters of the flow field (rotation speed, rocking mode) and electric field (electric field strength, direction of electric field, DC bias voltage, AC frequency), the force balance and motion trajectory of nanoparticles of different sizes, charges and polarities near the separation membrane 12 can be dynamically adjusted, thereby achieving synergistic selective separation of specific components in complex mixtures based on multiple parameters (size + charge). Its separation accuracy and flexibility far exceed those of single physical field methods.

[0033] The system monitors transmembrane flux and current signals in real time, and dynamically adjusts flow and electric field parameters accordingly to achieve optimal separation or antifouling. After operation, samples are recovered from both half-chambers for subsequent characterization using TEM and FTIR, as well as concentration analysis.

[0034] The nanoparticles of this invention refer to solid or soft matter particles with at least one dimension ranging from 1 to 1000 nm, including the following types: (1) Environmental colloidal particles, such as natural inorganic colloids (clay minerals, iron and aluminum oxides), organic colloids (humic acid, polysaccharides, etc.) and their complexes in soil, sediments, and water bodies. (2) Engineering and material nanoparticles, such as metal nanoparticles (gold, silver, zero-valent iron), metal oxides (titanium dioxide, zinc oxide, silicon dioxide), carbon-based materials (carbon nanotubes, graphene quantum dots), polymer microspheres, etc. (3) Bio-derived nanoparticles, such as viruses, exosomes, protein aggregates, and liposomes, etc. (4) Pollutant carrier particles, which are composite pollutant particles formed by adsorbing heavy metal ions, organic pollutants (such as polycyclic aromatic hydrocarbons, pesticides) or nutrients on the surface of the above-mentioned particles.

[0035] The nanoparticle separation method provided in this embodiment includes installing a separation membrane 12 and configuring the flow field and electric field parameters of the nanoparticle separation device. The sample to be measured is loaded into the nanoparticle separation device, and the device is started to provide a flow field and electric field to the sample to separate the nanoparticles. The parameter changes of the electrophoretic separation device 3 are monitored, and the flow field and electric field parameters of the electrophoretic separation device 3 are adjusted. The sample to be measured is recovered from the half-chambers on both sides of the electrophoretic separation device 3. A flow field is provided by shaking and rotation. The inner cavity of the original chamber 8 is designed with a shallow dish-shaped guide surface, allowing the sample liquid to form a uniform liquid layer during rotation, thereby covering the entire surface of the separation membrane 12. A high-precision shaker 2 generates shaking, which creates a local high-shear flow on the surface of the separation membrane 12, effectively disturbing the boundary layer, preventing particle deposition on the surface of the separation membrane 12, alleviating concentration polarization and membrane fouling, and maintaining long-term stability of the separation flux. Applying an electric field to drive charged nanoparticles to undergo electrophoretic motion can actively drive particles toward or away from the surface of the separation membrane 12, thereby achieving pre-enrichment or repulsion of particles with specific charges, thus increasing the transmembrane flux of target particles and reducing the membrane adsorption of pollutants.

[0036] Example 2 like Figure 2 As shown, this embodiment provides a nanoparticle separation device based on electric field and flow field driven, which is applied to the nanoparticle separation method based on electric field and flow field driven in Embodiment 1. The device includes a batch adsorption device 1, a shaker 2 connected to the bottom of the batch adsorption device 1, and an electrophoretic separation device 3 electrically connected to the batch adsorption device 1.

[0037] The batch adsorption device 1 includes two opposing drive frames 4, a support frame 5 is provided between the two drive frames 4, and an adsorption unit 6 is provided inside the support frame 5.

[0038] The distance between the two drive frames 4 is greater than the length of the adsorption unit 6. Each drive frame 4 is connected to 10 support frames 5, and each support frame 5 is equipped with one adsorption unit 6. Therefore, the batch adsorption device 1 of the present invention can fix 60 adsorption units 6 at one time.

[0039] like Figure 3 As shown, the adsorption unit 6 includes a first fixing plate 7, a second fixing plate 16, and a separation membrane 12. A primary chamber 8 is provided between the first fixing plate 7 and the separation membrane 12, and a receiving chamber 9 is provided between the second fixing plate 16 and the separation membrane 12. Both the first fixing plate 7 and the second fixing plate 16 are annular structures.

[0040] The nanoparticle separation device driven by electric field and flow field also includes a gasket 13. A positive electrode 10 is installed in the original chamber 8 near the receiving chamber 9, and a negative electrode 11 is installed in the receiving chamber 9 near the original chamber 8. The separation membrane 12 is adapted to the gasket 13 and is installed in the gasket 13. The gasket 13 has a ring structure.

[0041] The inner cavities of the primary chamber 8 and the receiving chamber 9 are designed with shallow, dish-shaped flow-guiding surfaces, allowing the sample liquid to form a uniform liquid layer covering the entire surface of the separation membrane 12 during rotation. During operation, the sample suspension is injected into the primary chamber 8, and the receiving liquid is injected into the receiving chamber 9. Electrode interfaces are located on the half-walls of both the primary chamber 8 and the receiving chamber 9; the electrode interface of the primary chamber 8 is the positive electrode 10, and the electrode interface of the receiving chamber 9 is the negative electrode 11.

[0042] The separation membrane 12 is sandwiched within the channel of the adsorption unit 6, located between the primary chamber 8 and the receiving chamber 9. The separation membrane 12 is made of a chemically compatible material such as polyethersulfone (PES), polyvinylidene fluoride (PVDF), or mixed cellulose ester (MCE). The separation membrane 12 has a molecular weight cutoff of 1 kDa, 3 kDa, 10 kDa, 30 kDa, 100 kDa, 300 kDa, or 1000 kDa, and an absolute pore size of 1 nm, 10 nm, 30 nm, 50 nm, 100 nm, 220 nm, 450 nm, or 1000 nm. The separation membrane 12 can meet the above size requirements for either its molecular weight cutoff or absolute pore size, or both.

[0043] Sealing plugs 15 are inserted into both ends of the pipe of adsorption unit 6, and fixing screws 14 are inserted into the side wall of the pipe of adsorption unit 6. The fixing screws 14 penetrate the sealing plugs 15 and are used to fix the sealing plugs 15 to both ends of the pipe of adsorption unit 6. The sealing plugs 15 are used to seal the pipe of adsorption unit 6.

[0044] The gasket 13 has a ring structure, and the separation membrane 12 is snapped into the gasket 13. The gasket 13 is used to fix the separation membrane 12 in the pipe of the adsorption unit 6.

[0045] The shaker 2 drives the entire electric and fluid-driven nanoparticle separation device to perform multi-dimensional micro-oscillations or vibrations, with an amplitude of 0.1-5 mm and a frequency of 0.1-10 Hz. The oscillations or vibrations generated by the shaker 2 introduce strong local turbulence or shear forces at the membrane-liquid interface, continuously sweeping the surface of the separation membrane 12, physically preventing particle deposition and maintaining the permeability of the separation membrane 12.

[0046] The electrophoretic separation device 3 includes one or more pairs of independently pressure-controlled electrodes, a high-voltage power supply, and a control system. The electrode pairs are arranged in parallel in the two half-chambers of the adsorption unit 6. The electrodes are made of chemically inert platinum material. The control system applies a DC electric field or an AC / pulsed electric field. The DC electric field is used to direct the migration of particles via electrophoresis, while the AC / pulsed electric field is used to generate selective forces on particles of different sizes / dielectric constants via dielectric electrophoresis, or to flush the membrane surface through periodic reverse electroosmotic flow.

[0047] The nanoparticle separation device based on electric field and flow field driven provided in this embodiment has the following advantages: (1) Through the synergistic effect of electric field and flow field, multi-dimensional intelligent sieving based on size + charge and even dielectric properties is realized, and the separation capability of complex systems (such as protein aggregates and surface-modified particles) far exceeds that of single-size sieving. High-throughput and multi-condition parallel separation can be realized. The electric field force can actively accelerate the migration of target particles to the surface of separation membrane 12, and the shaking flow field can effectively suppress membrane fouling. The combination of the two makes the separation process of nanoparticles faster and can maintain long-term high-throughput operation. (2) By replacing the separation membrane 12 with different pore sizes, the precise screening of particles with specific sizes in the range of 1-1000 nm can be realized. (3) The mild dynamic separation mechanism and chemical inert material design avoid particle agglomeration, adsorption loss and denaturation. (4) The volume of the original chamber 8 and the receiving chamber 9 is 10 mL, which is larger than that of conventional devices, which is beneficial to sample recovery and subsequent characterization. The large-capacity design makes the amount of recovered sample sufficient and can be directly used for analysis of various instruments, realizing an integrated process from separation to characterization. (5) The shear force generated by shaking and the electroosmotic scouring effect generated by the pulse / AC electric field work synergistically to alleviate concentration polarization and particle deposition, significantly extend membrane life, and reduce cleaning frequency. The operation is standardized and reproducible. The modular design reduces human error and improves the comparability and reliability of experimental data.

[0048] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, apparatus, article, or method that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, apparatus, article, or method. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, apparatus, article, or method that includes that element.

[0049] The above description is only a preferred embodiment of this application and does not limit the patent scope of this application. Any equivalent structural or procedural changes made based on the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims

1. A method for separating nanoparticles based on electric field and flow field driven processes, characterized in that, include: Install the separation membrane and configure the flow field and electric field parameters of the nanoparticle separation device; The sample to be measured is loaded into the nanoparticle separation device, and the nanoparticle separation device is started to provide a flow field and an electric field to the sample to be measured in order to separate the nanoparticles in the sample. Monitor the parameter changes of the electrophoretic separation device, and adjust the flow field parameters and electric field parameters of the electrophoretic separation device accordingly; The sample to be measured is recovered from the half-chambers on both sides of the electrophoretic separation device.

2. The nanoparticle separation method based on electric field and flow field driven according to claim 1, characterized in that, The step of loading the sample to be measured into the nanoparticle separation device includes: Inject a suspension containing nanoparticles into the original chamber; The receiving liquid or blank solution is injected into the receiving chamber; the original chamber and the receiving chamber are located on opposite sides of the electrophoretic separation device.

3. The nanoparticle separation method based on electric field and flow field driven according to claim 2, characterized in that, The suspension also includes a liquid medium and a dispersant compatible with the nanoparticles; the liquid medium is deionized water or a buffer solution, and the dispersant is sodium hexametaphosphate or a surfactant.

4. The nanoparticle separation method based on electric field and flow field driven according to claim 1, characterized in that, The activation of the nanoparticle separation device provides a flow field and an electric field to the sample to be measured, including: Start the shaking table and electrophoretic separation device; Control the shaking of the shaker to generate shear force or turbulence at the interface between the separation membrane and the suspension; The electrophoretic separation device is controlled to generate an alternating electric field or pulse to drive the target particles to migrate toward the separation membrane.

5. The nanoparticle separation method based on electric field and flow field driven according to claim 1, characterized in that, The flow field parameters include rotational speed and oscillation mode, and the electric field parameters include DC bias voltage and AC frequency.

6. The nanoparticle separation method based on electric field and flow field driven according to claim 1, characterized in that, The nanoparticles are any one of environmental colloidal particles, engineering and material nanoparticles, bio-derived nanoparticles, and pollutant carrier particles.

7. A nanoparticle separation device based on electric field and flow field driven separation, applied to the nanoparticle separation method based on electric field and flow field driven separation as described in any one of claims 1-6, characterized in that, The device includes a batch adsorption unit, a shaking table connected to the bottom of the batch adsorption unit, and an electrophoretic separation unit electrically connected to the batch adsorption unit.

8. The nanoparticle separation device based on electric field and flow field driven according to claim 7, characterized in that, The batch adsorption device includes two opposing drive frames, with a support frame between the two drive frames, and an adsorption unit is disposed within the support frame.

9. The nanoparticle separation device based on electric field and flow field driven according to claim 7, characterized in that, The adsorption unit includes a first fixing plate, a second fixing plate, and a separation membrane. A primary chamber is provided between the first fixing plate and the separation membrane, and a receiving chamber is provided between the second fixing plate and the separation membrane. Both the first fixing plate and the second fixing plate are annular structures.

10. The nanoparticle separation device based on electric field and flow field driven according to claim 9, characterized in that, It also includes a gasket, wherein a positive electrode is installed in the original chamber near the receiving chamber, and a negative electrode is installed in the receiving chamber near the original chamber; the separation membrane is adapted to the gasket, the separation membrane is installed in the gasket, and the gasket has a ring structure.

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