Preparation process of straw-concentrate fungus enzyme synergistic double-stage fermentation cattle and sheep feed in cold region

Abstract

The invention relates to a preparation technology of a straw-concentrate fungus enzyme synergistic double-stage fermentation cattle and sheep feed in a cold region, and belongs to the field of feed production. Step 2, preparing a concentrate; step 3, preparing a mixture; step 4, carrying out aerobic fermentation on the mixture; step 5, converting aerobic fermentation of the mixture into anaerobic fermentation; and step 6, carrying out anaerobic fermentation on the mixture, and sub-packaging to obtain the finished feed. According to the invention, an aerobic-anaerobic two-stage fermentation mode is adopted and combined with a bacterium-enzyme integrated synergistic effect, so that the straw fiber degradation rate and the nutrition conversion rate are remarkably improved, the protein content of the finished feed is improved by 15% or above, the starch digestibility is improved by 30%-40%, and the energy utilization rate is improved by 25%-30%; the device adapts to various fermentation modes such as manual pile turning, groove type mechanization and three-dimensional tank type fermentation modes, can flexibly adjust the production scale, and is suitable for large-scale production of farms in cold regions and feed processing factories.

Description

Technical Field

[0001] This invention belongs to the field of feed production, specifically the preparation process of a two-stage fermentation method for cattle and sheep feed using straw-concentrate bacteria and enzymes in cold regions. Background Technology

[0002] Feed is the key material basis for cattle and sheep farming in cold regions during the cold season, directly affecting their healthy growth, production performance, and economic benefits. Winters in cold regions are long and cold, resulting in large accumulations of crop straw. Direct feeding of straw leads to problems such as low fiber degradation rates, poor feed intake, and nutritional imbalances in cattle and sheep. Furthermore, traditional feed fermentation processes require additional heating and insulation, resulting in high costs and making them unsuitable for large-scale production in cold regions. Current fermented feed technologies mostly focus on single fermentation modes, resulting in poor synergistic effects between bacteria and enzymes, and lack specific design for temperature control during winter in cold regions. This leads to long fermentation cycles, significant nutrient loss, and an inability to meet the comprehensive energy, protein, and mineral requirements of cattle and sheep. Summary of the Invention

[0003] This invention provides a preparation process for a two-stage fermentation of cattle and sheep feed using straw, concentrate, microorganisms, and enzymes in cold regions, in order to overcome the deficiencies in the existing technology.

[0004] This invention is achieved through the following technical solution: The preparation process of straw-concentrate microbial enzyme synergistic two-stage fermentation of cattle and sheep feed in cold regions includes the following steps: Step 1: Coarse material pretreatment; Step Two: Preparation of Refined Materials; Step 3: Preparation of the mixture; Step 4: The mixture undergoes aerobic fermentation; Step 5: Transition from aerobic fermentation to anaerobic fermentation of the mixture; Step Six: The finished feed product is packaged after the mixed feed undergoes anaerobic fermentation.

[0005] As described above, the preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation cattle and sheep feed includes the following step: the roughage pretreatment operation is as follows: select any one or any two or more of corn, rice, sorghum and wheat straw in any proportion, process them with a rubbing machine to a length of 0.4 to 4 cm, and adjust the straw moisture content to 55 to 65%.

[0006] 3. The preparation process of the cold region straw-concentrate bacteria enzyme synergistic two-stage fermentation cattle and sheep feed according to claim 1, characterized in that: the mass ratio of each substance in the concentrate preparation in step two is: 50-60 parts corn flour, 15-30 parts soybean meal, 5-10 parts wheat bran, and 5 parts compound mineral premix. Each substance is weighed according to the ratio and the moisture content of the concentrate is adjusted to 12%-15%.

[0007] The preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation cattle and sheep feed as described above, wherein the compound mineral premix includes the following substances in parts by weight: 100 parts calcium carbonate, 150 parts dicalcium phosphate, 5 parts sodium chloride, 2.5 parts ferrous sulfate, 2 parts zinc sulfate, 1.5 parts manganese sulfate, 0.4 parts copper sulfate, 0.6 parts sodium selenite, and 738 parts zeolite powder.

[0008] As described above, in the preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation cattle and sheep feed, in step three, the coarse straw obtained in step one and the concentrate obtained in step two are mixed at a mass ratio of 7-8:2-3, and premixed evenly using a TMR machine to obtain a mixture; a compound microbial enzyme preparation is added to the mixture, and the amount of the compound microbial enzyme preparation added is 0.1-0.5% of the mass ratio of the mixture.

[0009] The preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation cattle and sheep feed as described above, wherein the compound microbial enzyme preparation comprises an enzyme preparation and a microbial preparation; the enzyme preparation accounts for 10% to 20% by mass, and the enzyme preparation is any one or more of cellulase, hemicellulase, and pectinase in any proportion; the microbial preparation accounts for 80% to 90% by mass, and the microbial preparation is any one or more of lactic acid bacteria, Bacillus subtilis, and yeast in any proportion; the total number of viable bacteria in the microbial preparation is not less than 1×10⁻⁶. 9 CFU / g.

[0010] As described above, in the preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation of cattle and sheep feed, the aerobic fermentation environment temperature in step four is 30-40℃, and the fermentation time is 24-72 hours. During the fermentation process, the pile is turned for the first time when the pile temperature reaches 38-40℃, and then turned once every 2-3℃ increase in temperature thereafter, with a turning rate of 0.5-1m. 3 / min, during the turning process, ensure that the oxygen content of the mixture is maintained at 8% to 12%.

[0011] As described above, in the preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation cattle and sheep feed, the mixture that has completed aerobic fermentation in step five is transferred to a warm house in the cold region during winter. The warm house is insulated by solar energy or coal-fired hot air furnace. The insulation layer of the warm house is not less than 5cm thick, and the temperature difference between the inside and outside of the warm house is maintained at 20-30℃. After being transferred to the warm house, the temperature of the mixture is adjusted to 20-25℃ and then sealed. The sealing process is carried out by vacuum packing machine to vacuum seal, and the oxygen content in the sealed system is ≤0.5%.

[0012] As described above, in the preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation of cattle and sheep feed, the sealed mixture in step six undergoes anaerobic fermentation at a fermentation temperature of 10-20℃ for 7-10 days. During the fermentation process, the pH value is monitored regularly. When the pH value drops to 5.5-6.5, the ambient temperature can be lowered to 8-10℃ to maintain stability.

[0013] As described above, in the preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation of cattle and sheep feed, after the anaerobic fermentation in step six, the finished feed product is tested. The test standards for the finished feed product are: crude protein content ≥12%, acid detergent fiber content ≤30%, neutral detergent fiber content ≤45%, calcium content 0.8%~1.2%, phosphorus content 0.4%~0.6%, iron content ≥50mg / kg, zinc content ≥40mg / kg, corn starch digestibility increased by 30%~40%, and energy value 10~12MJ / kg.

[0014] The advantages of this invention are: This invention employs an aerobic-anaerobic dual-stage fermentation mode, combined with the synergistic effect of bacteria and enzymes, to significantly improve the degradation rate and nutrient conversion rate of straw fiber. The protein content of the finished feed is increased by more than 15%, starch digestibility is increased by 30% to 40%, and energy utilization rate is increased by 25% to 30%. This invention is specifically designed for temperature control in cold winter regions. Aerobic fermentation relies on warm room insulation and does not require additional heating. The packaging temperature for aerobic-anaerobic conversion is controlled at 20-25℃ to ensure the activity of anaerobic microorganisms and reduce production costs. In this invention, corn flour is added to the concentrate to enhance energy supply, and the compound mineral premix is ​​precisely formulated with calcium, phosphorus and trace elements to achieve balanced feed nutrition and meet the growth and fattening needs of cattle and sheep. This invention is compatible with various fermentation modes such as manual turning, trough mechanization, and three-dimensional tank fermentation, and can flexibly adjust the production scale, making it suitable for large-scale production in cold-region farms and feed processing plants. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of straw processing according to an embodiment of the present invention; Figure 2 This is a schematic diagram illustrating the mixing of straw and concentrate according to an embodiment of the present invention; Figure 3This is a schematic diagram of the aerobic fermentation of the mixture transfer according to an embodiment of the present invention; Figure 4 This is one of the schematic diagrams of aerobic fermentation of the mixture according to an embodiment of the present invention; Figure 5 This is a second schematic diagram of the aerobic fermentation of the mixture according to an embodiment of the present invention; Figure 6 This is a schematic diagram of temperature-controlled packaging of the mixture according to an embodiment of the present invention; Figure 7 This is a schematic diagram of the anaerobic fermentation of the mixture according to an embodiment of the present invention; Figure 8 This is a schematic diagram of the finished feed product prepared according to an embodiment of the present invention. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] The preparation process of straw-concentrate microbial enzyme synergistic two-stage fermentation of cattle and sheep feed in cold regions includes the following steps: Step 1: Coarse material pretreatment; Step Two: Preparation of Refined Materials; Step 3: Preparation of the mixture; Step 4: The mixture undergoes aerobic fermentation; Step 5: Transition from aerobic fermentation to anaerobic fermentation of the mixture; Step Six: The finished feed product is packaged after the mixed feed undergoes anaerobic fermentation.

[0019] Preferably, the coarse material pretreatment operation in step one of this embodiment is as follows: select any one or any two or more of corn, rice, sorghum and wheat straw in any proportion, process them with a rubbing machine to a length of 0.4 to 4 cm, and adjust the straw moisture content to 55 to 65%.

[0020] 3. The preparation process of the cold region straw-concentrate bacteria enzyme synergistic two-stage fermentation cattle and sheep feed according to claim 1, characterized in that: the mass ratio of each substance in the concentrate preparation in step two is: 50-60 parts corn flour, 15-30 parts soybean meal, 5-10 parts wheat bran, and 5 parts compound mineral premix. Each substance is weighed according to the ratio and the moisture content of the concentrate is adjusted to 12%-15%.

[0021] Preferably, the composite mineral premix described in this embodiment includes the following components in parts by weight: 100 parts calcium carbonate, 150 parts dicalcium phosphate, 5 parts sodium chloride, 2.5 parts ferrous sulfate, 2 parts zinc sulfate, 1.5 parts manganese sulfate, 0.4 parts copper sulfate, 0.6 parts sodium selenite, and 738 parts zeolite powder.

[0022] Preferably, in step three of this embodiment, the coarse straw obtained in step one and the refined straw obtained in step two are mixed at a mass ratio of 7-8:2-3, and premixed evenly using a TMR machine to obtain a mixture; a compound microbial enzyme preparation is added to the mixture, and the amount of the compound microbial enzyme preparation added is 0.1-0.5% of the mass ratio of the mixture.

[0023] Preferably, the compound microbial enzyme preparation described in this embodiment includes an enzyme preparation and a microbial preparation; the enzyme preparation accounts for 10% to 20% by mass, and the enzyme preparation is any one or more of cellulase, hemicellulase, and pectinase mixed in any proportion; the microbial preparation accounts for 80% to 90% by mass, and the microbial preparation is any one or more of lactic acid bacteria, Bacillus subtilis, and yeast mixed in any proportion; the total number of viable bacteria in the microbial preparation is not less than 1 × 10⁻⁶. 9 CFU / g.

[0024] Preferably, in step four of this embodiment, the aerobic fermentation environment temperature is 30–40°C, and the fermentation time is 24–72 hours. During fermentation, the pile is turned for the first time when the pile temperature reaches 38–40°C, and then turned once every 2–3°C increase in temperature, with a turning rate of 0.5–1 m / s. 3 / min, during the turning process, ensure that the oxygen content of the mixture is maintained at 8% to 12%.

[0025] Preferably, in step five of this embodiment, the mixture after aerobic fermentation is transferred to a warm house in a cold region during winter. The warm house is insulated by solar energy or a coal-fired hot air furnace, with an insulation layer thickness of not less than 5 cm. The temperature difference between the inside and outside of the warm house is maintained at 20-30°C. After being transferred to the warm house, the mixture temperature is adjusted to 20-25°C and then sealed. The sealing process is performed using a vacuum packing machine (using polyethylene vacuum packaging bags with a thickness of 0.15-0.2 mm and a vacuum degree of -0.06 to -0.08 MPa) or sealing in a silage pit (the silage pit is underground or semi-underground, with cement mortar plastering on the pit walls and a seepage prevention coefficient ≥1.0×10). -7 cm / s, the top of the pit is covered with a straw pad layer of ≥20cm + plastic film and compacted, and the oxygen content in the sealed system is ≤0.5%.

[0026] Preferably, in step six of this embodiment, the sealed mixture is subjected to anaerobic fermentation at a fermentation temperature of 10–20°C for 7–10 days. During the fermentation process, the pH value is monitored regularly. When the pH value drops to 5.5–6.5, the ambient temperature can be lowered to 8–10°C to maintain stability.

[0027] Preferably, in step six of this embodiment, after anaerobic fermentation, the finished feed product is tested. The testing standards for the finished feed product are: crude protein content ≥12%, acid detergent fiber (ADF) content ≤30%, neutral detergent fiber (NDF) content ≤45%, calcium content 0.8%~1.2%, phosphorus content 0.4%~0.6%, iron content ≥50mg / kg, zinc content ≥40mg / kg, corn starch digestibility increased by 30%~40%, and energy value 10~12MJ / kg, which is 25%~30% higher than that of unfermented mixed feed.

[0028] Example 1. Raw material pretreatment: Select corn and rice straw, mix them, and knead them until the length is 0.4-4cm (e.g., Figure 1 As shown), the moisture content is adjusted to 60%; the concentrate group includes the following substances in parts by weight: 50 parts corn flour, 30 parts soybean meal, 5 parts wheat bran, and 5 parts compound mineral premix (which includes the following substances in parts by weight: 100 parts calcium carbonate, 150 parts dicalcium phosphate, 5 parts sodium chloride, 2.5 parts ferrous sulfate, 2 parts zinc sulfate, 1.5 parts manganese sulfate, 0.4 parts copper sulfate, 0.6 parts sodium selenite, and 738 parts zeolite powder); straw and concentrate are mixed at a mass ratio of 70:30 (e.g. Figure 2 As shown in the figure, add 0.1% by weight of the compound bacterial enzyme preparation and mix well; 2. Aerobic fermentation (e.g.) Figure 3-5 (As shown): Manual turning of the compost is used, the temperature in the warm room is 30-40℃, the fermentation time is 72 hours, and the compost is turned once every 3℃ increase in temperature, while the oxygen content is maintained at 8%-12%; 3. Temperature-controlled packaging (e.g.) Figure 6 As shown): The temperature of the mixture is controlled to 25℃, vacuum-packed to -0.08MPa, and the thickness of the packaging bag is 0.2mm; 4. Anaerobic fermentation (e.g.) Figure 7 As shown): At an ambient temperature of 20℃, fermentation lasted for 10 days, and the pH value dropped to 5.5; on the 5th day of fermentation, the pH value was monitored at 6.5, and the temperature was lowered to 10℃ and maintained stable. After anaerobic fermentation, the finished feed product was obtained (e.g. Figure 8 (as shown) 5. Finished product testing: Crude protein content 14%, ADF content 25%, NDF content 40%, calcium content 1.2%, phosphorus content 0.6%, starch digestibility increased by 40%, energy value 12MJ / kg, and feed conversion ratio increased by 50%.

[0029] Verification test Experiment location: Kengyu Village, Fengle Town, Zhaozhou County, Daqing City, Heilongjiang Province; cattle farmer: Yu Moumou; Trial period: July 26, 2018 - November 26, 2018; Materials and Methods Beef cattle selection and grouping: 60 local crossbred cattle were selected and randomly divided into two groups, namely the experimental group and the control group, with 30 cattle in each group. The cattle were healthy, disease-free, and of similar weight and appearance, with a weight of about 400 kg. The experimental cattle were numbered, weighed, and treated with gastrointestinal medicine and deworming. Feed: The feed prepared in the example was used in the example; the control group used local fattening cattle feed (70% concentrate and 30% hay). Pre-trial transition: To reduce the stress response of cattle caused by feed change, a gradual feeding method was adopted to adapt to the experimental feed, with an adaptation period of 7 days; one-seventh of the original feed was replaced with the new feed every day for 7 days; Trial period setup and weighing: After the feed transition, weigh the animals and begin the formal trial; The trial period was set at 120 days, with a total of 3 weigh-ins: the first weigh-in at the beginning of the trial, the second weigh-in on day 30, and the third weigh-in on the day the trial ended. The average daily feed consumption during the breeding process is shown in Table 1. Table 1 Comparison of Daily Feed Consumption As shown in Table 1, comparing the feeding amounts of nutritional feed and conventional feed during the fattening of beef cattle at the same price, the experimental group saved 1.84 kg / head of feed per day compared to the control group, resulting in a total saving of 216 kg / head of feed over 120 days. The feed cost savings, calculated at approximately 1.0 yuan per kg of feed, translate to a reduction of 216 yuan per head of feed cost per cattle starting at 400 kg. Labor cost savings, calculated at 1 yuan per head of labor cost per day, result in a reduction of 120 yuan per head of labor cost over 120 days. In total, the comprehensive fattening cost per head of cattle can be reduced by 336 yuan during the 120-day trial period. Table 2 shows the statistical data on the weight of beef cattle during the breeding process; Table 2 Comparison of weight gain between the two groups of beef cattle As shown in Table 2, at the same price, the feed in the example group can achieve an additional 0.49 kg of weight gain per day for fattening cattle compared to the feed in the control group. Each fattening cattle in the example group can achieve a total weight gain of 201.6 kg over 120 days of feeding, while the average weight gain in the control group is only 142.8 kg. Each cattle in the example group can gain an additional 58.8 kg (including a weight difference of 10.66 kg). Calculated at 26 yuan per kilogram of beef cattle, the fattening cattle fed with nutritious feed can increase income by 1528.8 yuan per head. In summary, utilizing straw fermentation for nutritious feed can simultaneously reduce breeding costs and improve breeding efficiency. Furthermore, the "microbial-enzyme integration" technology greatly enriches the feed's nutritional diversity, enhances the digestive function and immunity of ruminants, thereby reducing the incidence of common digestive diseases and the need for medication, making livestock products green, organic, and safe. At the same time, the invention's process steps are clear, parameters are well-defined, and it is suitable for large-scale production in cold regions during winter. The required equipment is conventional for farms, the raw materials are readily available, and the cost is low, resulting in significant economic and social benefits.

[0030] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A preparation process for a two-stage fermentation of cattle and sheep feed using straw, concentrate, microorganisms, and enzymes in cold regions, characterized by: Includes the following steps: Step 1: Coarse material pretreatment; Step Two: Preparation of Refined Materials; Step 3: Preparation of the mixture; Step 4: The mixture undergoes aerobic fermentation; Step 5: Transition from aerobic fermentation to anaerobic fermentation of the mixture; Step Six: The finished feed product is packaged after the mixed feed undergoes anaerobic fermentation.

2. The preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation of cattle and sheep feed according to claim 1, characterized in that: The coarse material pretreatment operation in step one is as follows: select any one or more of corn, rice, sorghum and wheat straw in any proportion, process them with a rubbing machine to a length of 0.4 to 4 cm, and adjust the straw moisture content to 55 to 65%.

3. The preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation of cattle and sheep feed according to claim 1, characterized in that: The mass ratio of each substance in the preparation of the concentrate in step two is as follows: 50-60 parts corn flour, 15-30 parts soybean meal, 5-10 parts wheat bran, and 5 parts compound mineral premix. Weigh each substance according to the ratio and adjust the moisture content of the concentrate to 12%-15%.

4. The preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation cattle and sheep feed according to claim 3, characterized in that: The aforementioned composite mineral premix comprises the following components in parts by weight: 100 parts calcium carbonate, 150 parts dicalcium phosphate, 5 parts sodium chloride, 2.5 parts ferrous sulfate, 2 parts zinc sulfate, 1.5 parts manganese sulfate, 0.4 parts copper sulfate, 0.6 parts sodium selenite, and 738 parts zeolite powder.

5. The preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation of cattle and sheep feed according to claim 1, characterized in that: In step three, the coarse straw obtained in step one and the refined straw obtained in step two are mixed at a mass ratio of 7-8:2-3 and premixed evenly using a TMR machine to obtain a mixture; a compound microbial enzyme preparation is added to the mixture, and the amount of the compound microbial enzyme preparation added is 0.1-0.5% of the mass ratio of the mixture.

6. The preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation of cattle and sheep feed according to claim 5, characterized in that: The aforementioned compound microbial enzyme preparation comprises an enzyme preparation and a microbial preparation; the enzyme preparation accounts for 10% to 20% by mass, and is a mixture of any one or more of cellulase, hemicellulase, and pectinase in any proportion; the microbial preparation accounts for 80% to 90% by mass, and is a mixture of any one or more of lactic acid bacteria, Bacillus subtilis, and yeast in any proportion; the total viable count of the microbial preparation is not less than 1 × 10⁻⁶. 9 CFU / g.

7. The preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation of cattle and sheep feed according to claim 1, characterized in that: In step four, the aerobic fermentation environment temperature is 30–40℃, and the fermentation time is 24–72 hours. During fermentation, the pile is turned for the first time when the temperature reaches 38–40℃, and then turned once every 2–3℃ increase in temperature, with a turning rate of 0.5–1 m / s. 3 / min, during the turning process, ensure that the oxygen content of the mixture is maintained at 8% to 12%.

8. The preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation of cattle and sheep feed according to claim 1, characterized in that: In step five, the mixture that has undergone aerobic fermentation is transferred to a warm house in a cold region during winter. The warm house is insulated by solar energy or a coal-fired hot air furnace. The insulation layer of the warm house is no less than 5cm thick, and the temperature difference between the inside and outside of the warm house is maintained at 20-30℃. After being transferred to the warm house, the temperature of the mixture is adjusted to 20-25℃ and then sealed. The sealing process is carried out by vacuum sealing using a vacuum packing machine, and the oxygen content in the sealed system is ≤0.5%.

9. The preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation cattle and sheep feed according to claim 1, characterized in that: The sealed mixture in step six is ​​subjected to anaerobic fermentation at a temperature of 10–20°C for 7–10 days. During the fermentation process, the pH value is monitored regularly. When the pH value drops to 5.5–6.5, the ambient temperature can be lowered to 8–10°C to maintain stability.

10. The preparation process of the cold-region straw-concentrate microbial enzyme synergistic two-stage fermentation of cattle and sheep feed according to claim 1, characterized in that: After the anaerobic fermentation in step six is ​​completed, the finished feed product is tested. The testing standards for the finished feed product are as follows: crude protein content ≥12%, acid detergent fiber content ≤30%, neutral detergent fiber content ≤45%, calcium content 0.8%~1.2%, phosphorus content 0.4%~0.6%, iron content ≥50mg / kg, zinc content ≥40mg / kg, corn starch digestibility increased by 30%~40%, and energy value 10~12MJ / kg.

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