Preparation method of Chinese herbaceous peony root extract
By optimizing the extraction conditions and extract composition, the extraction efficiency of β-pentagalloyl glucose and oleanolic acid in peony root was improved, solving the problem of poor whitening and antioxidant effects of peony root extract, and achieving efficient and low-cost extraction results.
Patent Information
- Application Number
- CN202610176516.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-02-06
- Publication Date
- 2026-05-12
AI Technical Summary
Existing technologies have low efficiency in extracting β-pentagalloglucoside and oleanolic acid from peony roots, resulting in the whitening and antioxidant effects of peony root extract not meeting expectations.
Peony root was extracted using an extractant composed of ethanol-water solution, acetic acid, acetone, and chloroacetaldehyde under heating and pressure conditions. Specific parameters, including temperature, pressure, and time, were optimized to promote the dissolution of β-pentagalloglucoside and oleanolic acid.
The content of β-pentagalloglucoside and oleanolic acid was significantly increased, which significantly improved the whitening and antioxidant effects of peony root extract, and the process was simple and low-cost.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of skincare product raw materials, and in particular to a method for preparing peony root extract. Background Technology
[0002] As people's requirements for the safety of skincare product ingredients have increased, plant extracts have become one of the most common ingredients in skincare products. Different plant extracts contain different active ingredients, and these active ingredients can exert various skincare effects. Among them, peony extract has whitening and antioxidant effects and is one of the plant extracts commonly used in skincare products. The most abundant component in peony is paeoniflorin, which is one of the main components that contribute to whitening and antioxidant effects. However, peony also contains β-pentagalloglucoside and oleanolic acid. Both β-pentagalloglucoside and oleanolic acid have very significant whitening and antioxidant effects. The higher the content of β-pentagalloglucoside and oleanolic acid in peony extract, the more significant its whitening and antioxidant effects.
[0003] However, to control costs, inexpensive peony root is usually used as raw material during extraction. The theoretical mass content of β-pentagalloyl glucose in peony root is 0.1%, the theoretical mass content of oleanolic acid is 0.1%, and the theoretical mass content of paeoniflorin is 1.5%. It can be seen that the content of β-pentagalloyl glucose and oleanolic acid in peony root is much lower than that of paeoniflorin. Therefore, the extraction effect of rare β-pentagalloyl glucose and oleanolic acid is usually poor, resulting in a low content of β-pentagalloyl glucose and oleanolic acid in peony root extract. Consequently, the whitening and antioxidant effects of peony root extract do not meet expectations, and there is still room for improvement. Summary of the Invention
[0004] In order to extract more β-pentagalloyl glucose and oleanolic acid from peony root to further improve the whitening and antioxidant effects of peony root extract, this application provides a method for preparing peony root extract.
[0005] The method for preparing peony root extract provided in this application adopts the following technical solution: A method for preparing peony root extract includes the following steps: Step 1), add peony root into the extract, heat and pressurize to extract, and obtain the extract; Step 2), filter the extract, collect the filtrate, concentrate and dry the filtrate to obtain peony root extract rich in β-pentagalloglucoside and oleanolic acid; The extract is a mixture of aqueous ethanol, acetic acid, acetone, and chloroacetaldehyde.
[0006] By employing the above-mentioned technical solution, peony root is extracted under heating and pressure using an extraction solution composed of ethanol aqueous solution, acetic acid, acetone, and chloroacetaldehyde. This extraction solution effectively promotes the extraction and dissolution of β-pentagalloglucopyranosyl glucose and oleanolic acid from the peony root, resulting in high extraction efficiency. The obtained peony root extract contains a high content of β-pentagalloglucopyranosyl glucose and oleanolic acid, approaching its theoretical content. This significantly enhances the whitening and antioxidant effects of the peony root extract, better meeting the skincare efficacy requirements of skincare products. Furthermore, the extraction process is simple, requires no purification, and yields a high-quality extract at a low cost, making it economically valuable.
[0007] Preferably, the mass ratio of the ethanol aqueous solution, acetic acid, acetone and chloroacetaldehyde in the extract is 100:28-32:12-15:10-13.
[0008] By adopting the above technical solution and specifically selecting the mass ratio of ethanol aqueous solution, acetic acid, acetone and chloroacetaldehyde, the extraction and dissolution of β-pentagalloglucoside and oleanolic acid from peony root are better promoted, resulting in a higher content of β-pentagalloglucoside and oleanolic acid in peony root extract, making the whitening and antioxidant effects of peony root extract more significant.
[0009] Preferably, the ethanol aqueous solution contains 75-80% ethanol by mass.
[0010] By adopting the above technical solution and specifically selecting the mass content of ethanol in the ethanol-water solution, the combined effect with acetic acid, acetone, and chloroacetaldehyde is better, which significantly promotes the extraction and dissolution of β-pentagalloyl glucose and oleanolic acid from peony root in the extract, resulting in better product quality.
[0011] Preferably, in step 1), the extraction temperature is 44-48℃.
[0012] By adopting the above technical solution and selecting a special extraction temperature, it is more conducive to the extraction and dissolution of β-pentagalloglucoside and oleanolic acid from peony root in the extract.
[0013] Preferably, in step 1), the extraction pressure is 580-600 MPa.
[0014] By adopting the above technical solution and selecting a special extraction pressure, the effect of promoting the extraction and dissolution of β-pentagalloglucoside and oleanolic acid from peony root in the extract can be further improved.
[0015] Preferably, in step 1), the extraction time is 10-15 minutes.
[0016] By adopting the above technical solution and selecting an extraction time of 10-15 minutes, the extraction is more thorough, and energy and time waste is reduced, resulting in higher economic value.
[0017] Preferably, in step 1), the peony root is first crushed into 80-100 mesh peony root particles, and then the peony root particles are added to the extract.
[0018] By adopting the above technical solution, the contact area between the raw material and the extract can be increased through pulverization, thereby improving the extraction efficiency and resulting in higher economic value.
[0019] Preferably, in step 1), the mass ratio of peony root to extract is 1:10-20.
[0020] By adopting the above technical solution and specifically selecting the mass ratio of peony root to extract, the extraction effect is better, and the consumption of extract can be effectively controlled, reducing raw material waste and having high economic value.
[0021] In summary, this application has the following beneficial effects: 1. This application uses an extraction solution composed of ethanol aqueous solution, acetic acid, acetone, and chloroacetaldehyde to extract peony roots under heating and pressure. This extraction solution effectively promotes the extraction and dissolution of β-pentagalloglucopyranosyl glucose and oleanolic acid from the peony roots, resulting in high extraction efficiency. The obtained peony root extract contains a high content of β-pentagalloglucopyranosyl glucose and oleanolic acid, approaching its theoretical content. This significantly enhances the whitening and antioxidant effects of the peony root extract, better meeting the skincare efficacy requirements of skincare products. Furthermore, the extraction process is simple, requires no purification, and yields a high-quality extract at a low cost, making it economically valuable.
[0022] 2. In this application, it is preferred to select the specific mass ratio of ethanol aqueous solution, acetic acid, acetone and chloroacetaldehyde to promote the extraction and dissolution of β-pentagalloglucoside and oleanolic acid from peony root in the extract, which will result in a higher content of β-pentagalloglucoside and oleanolic acid in the peony root extract, making the whitening and antioxidant effects of peony root extract more significant.
[0023] 3. This application, by selecting special extraction temperature and extraction pressure, is more conducive to the extraction of β-pentagalloglucoside and oleanolic acid from peony root and their dissolution in the extract. Detailed Implementation
[0024] The present application will be further described in detail below with reference to the embodiments.
[0025] Example 1
[0026] A method for preparing peony root extract includes the following steps: Step 1): Crush the peony root into 80-mesh peony root particles. Mix 100 kg of 75% ethanol aqueous solution, 28 kg of acetic acid, 12 kg of acetone, and 10 kg of chloroacetaldehyde evenly to obtain an extract. Put 1 kg of peony root particles and 10 kg of extract into an ultra-high pressure extraction tank, stir continuously at 50 r / min, pressurize to 580 MPa, heat to 44℃, and extract for 10 min to obtain the extract.
[0027] Step 2), filter the extract, take the filtrate, concentrate the filtrate by rotary evaporation at 0.01 MPa and 50°C to a paste, and dry the paste by spray drying to obtain peony root extract rich in β-pentagalloglucoside and oleanolic acid.
[0028] The ethanol-water solution is prepared by combining commercially available anhydrous ethanol with a mass content of 95% with deionized water.
[0029] Acetic acid was obtained from commercial sources, CAS No.: 64-19-7.
[0030] Acetone was obtained from commercial sources, CAS No.: 67-64-1.
[0031] Chloroacetaldehyde is a commercially available product, CAS number: 107-20-0.
[0032] Peony roots are sourced from commercially available dried products.
[0033] The ultra-high pressure extraction tank, model HPP-100, was purchased from Baotou Kefa High Pressure Technology Co., Ltd.
[0034] Example 2
[0035] A method for preparing peony root extract includes the following steps: Step 1): Crush the peony root into 100-mesh peony root particles. Mix 100 kg of 80% ethanol aqueous solution, 30 kg of acetic acid, 13.5 kg of acetone, and 11.5 kg of chloroacetaldehyde evenly to obtain an extract. Put 1 kg of peony root particles and 15 kg of extract into an ultra-high pressure extraction tank, stir continuously at 55 r / min, pressurize to 590 MPa, heat to 46℃, and extract for 12 min to obtain the extract.
[0036] Step 2), filter the extract, take the filtrate, concentrate the filtrate by rotary evaporation at 0.01 MPa and 50°C to a paste, and dry the paste by spray drying to obtain peony root extract rich in β-pentagalloglucoside and oleanolic acid.
[0037] The ethanol-water solution is prepared by combining commercially available anhydrous ethanol with a mass content of 95% with deionized water.
[0038] Acetic acid was obtained from commercial sources, CAS No.: 64-19-7.
[0039] Acetone was obtained from commercial sources, CAS No.: 67-64-1.
[0040] Chloroacetaldehyde is a commercially available product, CAS number: 107-20-0.
[0041] Peony roots are sourced from commercially available dried products.
[0042] The ultra-high pressure extraction tank, model HPP-100, was purchased from Baotou Kefa High Pressure Technology Co., Ltd.
[0043] Example 3
[0044] A method for preparing peony root extract includes the following steps: Step 1): Crush the peony root into 100-mesh peony root particles. Mix 100 kg of 80% ethanol aqueous solution, 32 kg of acetic acid, 15 kg of acetone, and 13 kg of chloroacetaldehyde evenly to obtain an extract. Put 1 kg of peony root particles and 20 kg of extract into an ultra-high pressure extraction tank, stir continuously at 60 r / min, pressurize to 600 MPa, heat to 48℃, and extract for 15 min to obtain the extract.
[0045] Step 2), filter the extract, take the filtrate, concentrate the filtrate by rotary evaporation at 0.01 MPa and 50°C to a paste, and dry the paste by spray drying to obtain peony root extract rich in β-pentagalloglucoside and oleanolic acid.
[0046] The ethanol-water solution is prepared by combining commercially available anhydrous ethanol with a mass content of 95% with deionized water.
[0047] Acetic acid was obtained from commercial sources, CAS No.: 64-19-7.
[0048] Acetone was obtained from commercial sources, CAS No.: 67-64-1.
[0049] Chloroacetaldehyde is a commercially available product, CAS number: 107-20-0.
[0050] Peony roots are sourced from commercially available dried products.
[0051] The ultra-high pressure extraction tank, model HPP-100, was purchased from Baotou Kefa High Pressure Technology Co., Ltd.
[0052] Comparative Example 1 A method for preparing peony root extract, which differs from Example 2 only in that: In the extract, ethyl acetate was used to replace acetic acid in an equal amount.
[0053] Ethyl acetate was obtained from commercial sources, CAS No.: 141-78-6.
[0054] Comparative Example 2 A method for preparing peony root extract, which differs from Example 2 only in that: In the extract, methanol was used to replace acetone in an equal amount.
[0055] Methanol was sourced from commercially available sources, CAS No.: 67-56-1.
[0056] Comparative Example 3 A method for preparing peony root extract, which differs from Example 2 only in that: In the extract, chloroform was used to replace chloroacetaldehyde in an equal amount.
[0057] chloroform is commercially available, CAS number: 67-66-3.
[0058] Comparative Example 4 A method for preparing peony root extract, which differs from Example 2 only in that: In the extract, ethyl acetate was used to replace acetic acid in equal amounts, methanol was used to replace acetone in equal amounts, and chloroform was used to replace chloroacetaldehyde in equal amounts.
[0059] Ethyl acetate was obtained from commercial sources, CAS No.: 141-78-6.
[0060] Methanol was sourced from commercially available sources, CAS No.: 67-56-1.
[0061] chloroform is commercially available, CAS number: 67-66-3.
[0062] Comparative Example 5 A method for preparing peony root extract, which differs from Example 2 only in that: Step 1) Crush the peony root into 100-mesh peony root particles. Mix 100 kg of 80% ethanol aqueous solution, 40 kg of acetic acid, 10 kg of acetone and 5 kg of chloroacetaldehyde evenly to obtain an extract. Put 1 kg of peony root particles and 15 kg of extract into an ultra-high pressure extraction tank, stir continuously at 55 r / min, pressurize to 590 MPa, heat to 46℃ and extract for 12 min to obtain the extract.
[0063] That is, the proportions of each component in the extract of this comparative example are different from those in the example.
[0064] Comparative Example 6 A method for preparing peony root extract, which differs from Example 2 only in that: Step 1): Crush the peony root into 100-mesh peony root particles. Mix 100 kg of 80% ethanol aqueous solution, 20 kg of acetic acid, 20 kg of acetone, and 5 kg of chloroacetaldehyde evenly to obtain an extract. Put 1 kg of peony root particles and 15 kg of extract into an ultra-high pressure extraction tank, stir continuously at 55 r / min, pressurize to 590 MPa, heat to 46℃, and extract for 12 min to obtain the extract.
[0065] That is, the proportions of each component in the extract of this comparative example are different from those in the example.
[0066] Comparative Example 7 A method for preparing peony root extract, which differs from Example 2 only in that: Step 1): Crush the peony root into 100-mesh peony root particles. Mix 100 kg of 80% ethanol aqueous solution, 20 kg of acetic acid, 10 kg of acetone, and 25 kg of chloroacetaldehyde evenly to obtain an extract. Put 1 kg of peony root particles and 15 kg of extract into an ultra-high pressure extraction tank, stir continuously at 55 r / min, pressurize to 590 MPa, heat to 46℃, and extract for 12 min to obtain the extract.
[0067] That is, the proportions of each component in the extract of this comparative example are different from those in the example.
[0068] Comparative Example 8 A method for preparing peony root extract includes the following steps: Step 1) Crush the peony root into 100-mesh peony root particles. Mix 100 kg of 80% ethanol aqueous solution, 30 kg of acetic acid, 13.5 kg of acetone and 11.5 kg of chloroacetaldehyde evenly to obtain an extract. Put 1 kg of peony root particles and 15 kg of extract into an ultra-high pressure extraction tank, stir continuously at 55 r / min, pressurize to 400 MPa, heat to 46℃, and extract for 12 min to obtain the extract.
[0069] That is, the extraction pressure in this comparative example is different from that in the example.
[0070] Comparative Example 9 A method for preparing peony root extract includes the following steps: Step 1) Crush the peony root into 100-mesh peony root particles. Mix 100 kg of 80% ethanol aqueous solution, 30 kg of acetic acid, 13.5 kg of acetone, and 11.5 kg of chloroacetaldehyde evenly to obtain an extract. Put 1 kg of peony root particles and 15 kg of extract into an ultra-high pressure extraction tank, stir continuously at 55 r / min, pressurize to 500 MPa, heat to 46℃, and extract for 12 min to obtain the extract.
[0071] That is, the extraction pressure in this comparative example is different from that in the example.
[0072] Comparative Example 10 A method for preparing peony root extract includes the following steps: Step 1) Crush the peony root into 100-mesh peony root particles. Mix 100 kg of 80% ethanol aqueous solution, 30 kg of acetic acid, 13.5 kg of acetone and 11.5 kg of chloroacetaldehyde evenly to obtain an extract. Put 1 kg of peony root particles and 15 kg of extract into an ultra-high pressure extraction tank, stir continuously at 55 r / min, pressurize to 590 MPa, heat to 40℃, and extract for 12 min to obtain the extract.
[0073] That is, the extraction temperature in this comparative example is different from that in the example.
[0074] Comparative Example 11 A method for preparing peony root extract includes the following steps: Step 1) Crush the peony root into 100-mesh peony root particles. Mix 100 kg of 80% ethanol aqueous solution, 30 kg of acetic acid, 13.5 kg of acetone and 11.5 kg of chloroacetaldehyde evenly to obtain an extract. Put 1 kg of peony root particles and 15 kg of extract into an ultra-high pressure extraction tank, stir continuously at 55 r / min, pressurize to 590 MPa, heat to 55℃, and extract for 12 min to obtain the extract.
[0075] That is, the extraction temperature in this comparative example is different from that in the example.
[0076] Experiment 1 The contents of β-pentagalloglucoside, oleanolic acid, and paeoniflorin in the peony root extracts of each example and comparative example were determined by HPLC.
[0077] 1. Determination of paeoniflorin content: Preparation of the reference solution: Take an appropriate amount of paeoniflorin reference standard, accurately weigh it, and add methanol to prepare solutions containing 1, 5, 10, 20, 40, 60, 80, and 100 μg per ml, respectively.
[0078] Preparation of the test solution: Take 0.1g of peony root extract, place it in a 50ml volumetric flask, add 35ml of dilute ethanol, sonicate (power 240W, frequency 45kHz) for 30 minutes, cool, add dilute ethanol to the mark, shake well, filter, and collect the filtrate to obtain the extract.
[0079] Determination method: Precisely pipette 10 μl of each concentration of the reference solution and inject it into the liquid chromatograph for determination.
[0080] Plot the peak height on the ordinate and the concentration on the abscissa. Then, draw points on the coordinate axis based on the peak height and concentration of each concentration reference standard. Connect the points to create a peak height-concentration standard curve.
[0081] Accurately pipette 10 μl of the test solution and inject it into the liquid chromatograph for determination. Determine the peak expressing paeoniflorin in the test sample based on the peak position of the reference standard. Then compare the peak height of the peak with the standard curve to obtain the concentration of paeoniflorin in the test sample.
[0082] The paeoniflorin content in the peony root extract is obtained by dividing the concentration of paeoniflorin in the test sample by the concentration of peony root extract in the test sample. The data is rounded to two decimal places.
[0083] Testing conditions: HPLC column: Thermo BDS Hypersil C18 100 × 4.6 mm 2.4 μm.
[0084] Mobile phase A: acetonitrile, mobile phase B: 0.1% phosphoric acid aqueous solution, the mass ratio of mobile phase A to mobile phase B is 14:86.
[0085] The gradient elution conditions are detailed in Table 1: Table 1
[0086] Flow rate: 1.0 ml / min; column temperature: 35℃; wavelength: UV-230 nm.
[0087] Injection concentration: 25 μg / ml; Injection volume: 10 μL; Dissolution method: 80% methanol.
[0088] 2. Determination of β-pentagalloglucoside content: Preparation of the reference solution: Take an appropriate amount of β-pentagalloglucopyranoside reference standard, accurately weigh it, and add methanol to prepare solutions containing 1, 5, 10, 20, 40, 60, 80, and 100 μg per ml, respectively.
[0089] Preparation of the test solution: Take 0.1g of peony root extract, place it in a 50ml volumetric flask, add 35ml of dilute ethanol, sonicate (power 240W, frequency 45kHz) for 30 minutes, cool, add dilute ethanol to the mark, shake well, filter, and collect the filtrate to obtain the extract.
[0090] Determination method: Precisely pipette 10 μl of each concentration of the reference solution and inject it into the liquid chromatograph for determination.
[0091] Plot the peak height on the ordinate and the concentration on the abscissa. Then, draw points on the coordinate axis based on the peak height and concentration of each concentration reference standard. Connect the points to create a peak height-concentration standard curve.
[0092] Accurately pipette 10 μl of the test solution and inject it into the liquid chromatograph for determination. Determine the peak expressing β-pentagalloglucopyranosylglucose in the test sample based on the peak position of the reference standard. Then compare the peak height of the peak with the standard curve to obtain the concentration of β-pentagalloglucopyranosylglucose in the test sample.
[0093] The content of β-pentagalloglucopyranosyl glucose in peony root extract is obtained by dividing the concentration of β-pentagalloglucopyranosyl glucose in the test sample by the concentration of peony root extract in the test sample. The data is rounded to two decimal places.
[0094] Testing conditions: HPLC column: Thermo BDS Hypersil C18 100 × 4.6 mm 2.4 μm.
[0095] Mobile phase A: acetonitrile, mobile phase B: 0.1% phosphoric acid aqueous solution, the mass ratio of mobile phase A to mobile phase B is 14:86.
[0096] The gradient elution conditions are detailed in Table 2: Table 2
[0097] Flow rate: 1.0 ml / min; column temperature: 35℃; wavelength: UV-280 nm.
[0098] Injection concentration: 25 μg / ml; Injection volume: 10 μL; Dissolution method: 80% methanol.
[0099] 3. Determination of oleanolic acid content: Preparation of the reference solution: Take an appropriate amount of oleanolic acid reference standard, accurately weigh it, and add methanol to prepare solutions containing 1, 5, 10, 20, 40, 60, 80, and 100 μg per ml, respectively.
[0100] Preparation of the test solution: Take 0.1g of peony root extract, place it in a 50ml volumetric flask, add 35ml of dilute ethanol, sonicate (power 240W, frequency 45kHz) for 30 minutes, cool, add dilute ethanol to the mark, shake well, filter, and collect the filtrate to obtain the extract.
[0101] Determination method: Precisely pipette 10 μl of each concentration of the reference solution and inject it into the liquid chromatograph for determination.
[0102] Plot the peak height on the ordinate and the concentration on the abscissa. Then, draw points on the coordinate axis based on the peak height and concentration of each concentration reference standard. Connect the points to create a peak height-concentration standard curve.
[0103] Accurately pipette 10 μl of the test solution and inject it into the liquid chromatograph for determination. Determine the peak expressing oleanolic acid in the test sample based on the peak position of the reference standard. Then compare the peak height of the peak with the standard curve to obtain the concentration of oleanolic acid in the test sample.
[0104] The content of oleanolic acid in peony root extract is obtained by dividing the concentration of oleanolic acid in the test sample by the concentration of peony root extract in the test sample. The data is rounded to two decimal places.
[0105] Testing conditions: HPLC column: Thermo BDS Hypersil C18 100 × 4.6 mm 2.4 μm.
[0106] Mobile phase A: acetonitrile, mobile phase B: 0.1% phosphoric acid aqueous solution, the mass ratio of mobile phase A to mobile phase B is 14:86.
[0107] The gradient elution conditions are detailed in Table 3: Table 3
[0108] Flow rate: 1.0 ml / min; column temperature: 25℃; wavelength: UV-205 nm.
[0109] Injection concentration: 20 μg / ml; Injection volume: 10 μL; Dissolution method: 80% methanol.
[0110] The experimental data are detailed in Table 4.
[0111] Table 4
[0112] As shown in Table 4, the paeoniflorin content in the extracts of each embodiment and comparative example is similar. However, the β-pentagalloglucoside and oleanolic acid content in each embodiment is significantly higher than that in the comparative examples. This proves that the extract prepared by compounding ethanol aqueous solution, acetic acid, acetone and chloroacetaldehyde in a specific ratio, under specific extraction temperature and pressure, can more fully extract β-pentagalloglucoside and oleanolic acid, thereby increasing the content of β-pentagalloglucoside and oleanolic acid in the extract, and thus significantly improving the whitening and antioxidant effects of peony root extract.
[0113] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.
Claims
1. A method for preparing peony root extract, characterized in that: Includes the following steps: Step 1), add peony root into the extract, heat and pressurize to extract, and obtain the extract; Step 2), filter the extract, collect the filtrate, concentrate and dry the filtrate to obtain peony root extract rich in β-pentagalloglucoside and oleanolic acid; The extract is a mixture of aqueous ethanol, acetic acid, acetone, and chloroacetaldehyde.
2. The method for preparing peony root extract according to claim 1, characterized in that: The mass ratio of ethanol aqueous solution, acetic acid, acetone and chloroacetaldehyde in the extract is 100:28-32:12-15:10-13.
3. The method for preparing peony root extract according to claim 2, characterized in that: The ethanol aqueous solution contains 75-80% ethanol by mass.
4. A method for preparing a peony root extract according to any one of claims 1-3, characterized in that: In step 1), the extraction temperature is 44-48℃.
5. The method for preparing peony root extract according to claim 4, characterized in that: In step 1), the extraction pressure is 580-600 MPa.
6. The method for preparing peony root extract according to claim 5, characterized in that: In step 1), the extraction time is 10-15 minutes.
7. The method for preparing peony root extract according to claim 6, characterized in that: In step 1), the peony root is first crushed into 80-100 mesh peony root particles, and then the peony root particles are added to the extract.
8. The method for preparing peony root extract according to claim 7, characterized in that: In step 1), the mass ratio of peony root to extract is 1:10-20.