Microbial separation device for microbial inspection

Abstract

The invention discloses a microbiological separation device for microbiological examination, and relates to the technical field of microbiological separation, and the microbiological separation device is technically characterized by comprising a shell, the lower end of the shell is fixedly connected with a discharge pipe, the inner wall of the shell is provided with a separation assembly, and the upper end of the shell is provided with a pretreatment assembly. A motor I drives a shaft rod to rotate to drive a rotating rod to revolve around the shaft rod, and meanwhile, a gear rolls along a tooth groove in a circular ring in a meshing manner to realize rotation of the rotating rod, so that stirring blades perform three-dimensional stirring on a culture solution, and in cooperation with contact sweeping of a brush strip and the inner bottom wall of the treatment tank, microorganisms deposited on the inner bottom wall of the treatment tank can be stirred; meanwhile, the stirring blades abut against the impact ball in the rotating process, horizontal reciprocating vibration is achieved under the action of the reset spring, no-dead-corner stirring of the culture solution is achieved, follow-up separation is facilitated, and the situation that the separation effect is affected due to uneven local concentration is avoided.

Description

Technical Field

[0001] This invention relates to the field of microbial isolation technology, specifically to a microbial isolation device for microbial testing. Background Technology

[0002] Microorganisms are a large group of organisms including bacteria, viruses, fungi, as well as some small protozoa and microalgae. They are tiny in size, closely related to humans, and encompass a wide range of species, both beneficial and harmful. They are widely involved in many fields such as food, medicine, industry, agriculture, environmental protection, and sports. Because different microorganisms have different requirements for energy, nutrition, and physicochemical conditions, existing culture media can be used for the isolation and culture of new microorganisms after appropriate optimization and modification. Microbial isolation and culture technology plays an important role in the study of microbial environmental functions, the elucidation of metabolic pathways, the verification of specific functions, and the application of basic experiments and production practices.

[0003] Currently, most existing microbial separation devices employ centrifugal separation. However, when using these devices, the microbial culture medium is in a static or transported state, and some microbial particles and nutrients tend to settle at the bottom of the container. The pretreatment structure of existing devices is often a single stirring blade, which can only achieve local stirring and cannot fully disperse the sediment at the bottom of the container. This makes it difficult for the deposited microorganisms to be evenly dispersed in the culture medium. This not only causes uneven distribution of microorganisms during subsequent separation, affecting separation accuracy, but may also cause the separation structure to be blocked by sediment, reducing separation efficiency and even leading to deviations in separation results, thus affecting the validity of the test data. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a microbial isolation device for microbial testing to achieve the aforementioned objectives.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a microbial separation device for microbial testing, comprising: a shell, a discharge pipe fixedly connected to the lower end of the shell, a separation component disposed on the inner wall of the shell, and a pretreatment component disposed at the upper end of the shell;

[0006] The pretreatment assembly includes a treatment tank. Two mounting brackets are fixedly connected to the outer wall of the treatment tank, and the lower ends of the two mounting brackets are fixedly connected to the upper end of the housing. A ring is fixedly connected to the inner wall of the treatment tank, and a toothed groove is formed at the upper end of the ring. A shaft is fixedly connected to the inner wall of the treatment tank, and two rotating rods are rotatably connected to the outer wall of the shaft. Gears are fixedly connected to the outer walls of the two rotating rods at their opposite ends, and both gears are meshed with the toothed grooves. Two symmetrically distributed sliding sleeves are slidably connected to the outer walls of the rotating rods, and a connecting rod is fixedly connected between the two sliding sleeves. A stirring blade is fixedly connected to the outer wall of the sliding sleeve, and a brush strip is fixedly connected to the end of the stirring blade away from the sliding sleeve.

[0007] Preferably, the upper end of the processing tank is fixedly connected to a feed inlet, the upper end of the processing tank is fixedly connected to a frame, the upper end of the frame is fixedly connected to a motor, the output end of the motor is fixedly connected to a shaft via a coupling, and the lower end of the processing tank is fixedly connected to a connecting pipe that communicates with the shell.

[0008] Preferably, the pretreatment assembly further includes a fixed sleeve fixedly connected to the outer wall of the rotating rod, a return spring fixedly connected to one side of the fixed sleeve, the end of the return spring away from the fixed sleeve being fixedly connected to one of the sliding sleeves, and two connecting rods fixedly connected to the outer wall of the shaft, with impact balls fixedly connected to the ends of the two connecting rods that are far apart from each other.

[0009] Preferably, the separation assembly includes a separation tank, the outer wall of which is provided with a plurality of annular grooves at equal intervals, a rotating rod is fixedly connected to the central axis of the inner wall of the separation tank, the rotating rod is rotatably connected to the inner wall of the housing, a pulley is fixedly connected to one end of the rotating rod that passes through the outer wall of the housing, a mounting base is fixedly connected to the upper end of the housing, a motor is fixedly connected to the upper end of the mounting base, a pulley is fixedly connected to the output end of the motor, and a drive belt is installed between the pulley and the pulley.

[0010] Preferably, the upper end of the separation tank is open, and the outer side wall of the separation tank has multiple separation holes, which are distributed in a circumferential array.

[0011] Preferably, the impact ball is located on one side of the stirring blade, and the stirring blade contacts the impact ball when it rotates.

[0012] Preferably, the inner wall of the housing is provided with a plurality of annular grooves II corresponding to annular groove I, and ball bearings are movably disposed on the inner wall of the plurality of annular grooves II, and the ball bearings are movably connected to annular groove I.

[0013] Preferably, a bracket is fixedly connected to the lower end of the housing, and a switch valve is installed on the outer side wall of the discharge pipe, the inlet, and the connecting pipe. An observation window is installed on the outer side wall of the housing.

[0014] Compared with the prior art, the present invention provides a microbial isolation device for microbial testing, which has the following beneficial effects:

[0015] The motor drives the shaft to rotate, causing the rotating rod to revolve around the shaft. Simultaneously, gears mesh and roll along the tooth grooves on the ring, thus rotating the rotating rod itself. This causes the stirring blades to agitate the culture medium in three dimensions. In conjunction with the brush strips contacting and sweeping the bottom wall of the treatment tank, the microorganisms deposited on the bottom wall of the treatment tank can be stirred, preventing sedimentation and accumulation. At the same time, the stirring blades collide with the impact ball during rotation, and under the action of the return spring, they achieve horizontal reciprocating vibration, achieving agitation of the culture medium without dead zones. This facilitates subsequent separation and avoids the separation effect being affected by uneven local concentrations.

[0016] The separation tank is rotated by a pulley driven by a motor, generating centrifugal force that allows microorganisms to be separated into the shell through the separation holes. At the same time, multiple separation holes further enhance the separation rate. In addition, the ball bearings between the annular groove one on the outside of the separation tank and the annular groove two on the inner wall of the shell can effectively buffer the vibration during centrifugal rotation and reduce operating noise. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0018] Figure 2 This is a schematic diagram of the cross-sectional structure of the housing of the present invention;

[0019] Figure 3 This is a schematic cross-sectional view of the processing tank of the present invention;

[0020] Figure 4 This is a schematic diagram of the stirring blade structure of the present invention;

[0021] Figure 5 For the present invention Figure 4 Enlarged schematic diagram of the structure at point A in the middle;

[0022] Figure 6 This is a schematic diagram of the separation component structure of the present invention.

[0023] In the diagram: 1. Shell; 2. Discharge pipe; 3. Separation assembly; 4. Pretreatment assembly; 401. Processing tank; 402. Mounting bracket; 403. Ring; 404. Gear groove; 405. Shaft; 406. Rotating rod; 407. Gear; 408. Sliding sleeve; 409. Connecting rod; 410. Stirring blade; 411. Brush strip; 4011. Inlet; 4012. Frame; 4013. Motor 1; 4014. Connecting pipe; 41. Fixing sleeve; 42. Return spring; 43. Connecting rod; 44. Impact ball; 301. Separation tank; 302. Ring groove 1; 303. Rotating rod; 304. Pulley 1; 305. Mounting base; 306. Motor 2; 307. Pulley 2; 308. Drive belt; 3011. Separation hole; 5. Ring groove 2; 6. Ball bearing; 7. Bracket; 8. Observation window. Detailed Implementation

[0024] In this invention, unless otherwise stated, the directional terms such as "up" and "down" generally refer to the directions shown in the accompanying drawings, or to the vertical, perpendicular, or gravitational direction; similarly, for ease of understanding and description, "left" and "right" generally refer to the left and right shown in the accompanying drawings; "inner" and "outer" refer to the inner and outer contours of each component itself, but the above directional terms are not intended to limit this invention.

[0025] Please see Figure 1-6 The present invention provides a technical solution for a microbial separation device for microbial testing: including: a shell 1, a discharge pipe 2 fixedly connected to the lower end of the shell 1, a separation component 3 provided on the inner wall of the shell 1, and a pretreatment component 4 provided on the upper end of the shell 1;

[0026] The pretreatment assembly 4 includes a treatment tank 401. Two mounting brackets 402 are fixedly connected to the outer wall of the treatment tank 401. The lower ends of the two mounting brackets 402 are fixedly connected to the upper end of the housing 1. A ring 403 is fixedly connected to the inner wall of the treatment tank 401. A toothed groove 404 is formed at the upper end of the ring 403. A shaft 405 is fixedly connected to the inner wall of the treatment tank 401. Two rotating rods 406 are rotatably connected to the outer wall of the shaft 405. Gears 407 are fixedly connected to the outer walls of the two rotating rods 406 at their opposite ends. All are engaged with the toothed groove 404. Two symmetrically distributed sliding sleeves 408 are slidably connected to the outer wall of the rotating rod 406. A connecting rod 409 is fixedly connected between the two sliding sleeves 408. A stirring blade 410 is fixedly connected to the outer wall of the sliding sleeve 408. A brush strip 411 is fixedly connected to the end of the stirring blade 410 away from the sliding sleeve 408, and one end of the brush strip 411 is bristles. When the stirring blade 410 rotates, the bristles on the brush strip 411 contact the inner bottom wall of the processing tank 401. In addition, the stirring blade 410 will not collide with the ring 403 when it rotates. The gear 407 can rotate along the toothed groove 404 on the ring 403, thereby driving the rotating rod 406 to rotate. The rotation of the rotating rod 406 drives the stirring blade 410 to rotate. The rotation of the stirring blade 410 drives the brush 411 to rotate, which can stir the microbial culture medium on the bottom wall of the treatment tank 401, preventing the microbial culture medium from settling at the bottom of the treatment tank 401. At the same time, the stirred microbial culture medium is easier to separate quickly in the future.

[0027] A feed inlet 4011 is fixedly connected to the upper end of the processing tank 401. A frame 4012 is also fixedly connected to the upper end of the processing tank 401, and a motor 4013 is fixedly connected to the upper end of the frame 4012. The output end of the motor 4013 is fixedly connected to a shaft 405 via a coupling. A connecting pipe 4014, which communicates with the shell 1, is fixedly connected to the lower end of the processing tank 401, with the lower end of the connecting pipe 4014 positioned above the separation tank 301. Unseparated microbial culture medium is injected into the processing tank 401 through the feed inlet 4011, and the output shaft 405 of the motor 4013 rotates. The stirred microbial culture medium can be transported into the separation tank 301 through the connecting pipe 4014.

[0028] The pretreatment assembly 4 also includes a fixed sleeve 41 fixedly connected to the outer wall of the rotating rod 406. A return spring 42 is fixedly connected to one side of the fixed sleeve 41. The end of the return spring 42 away from the fixed sleeve 41 is fixedly connected to one of the sliding sleeves 408. Two connecting rods 43 are fixedly connected to the outer wall of the shaft 405. The ends of the two connecting rods 43 that are far apart from each other are fixedly connected to an impact ball 44. The return spring 42 facilitates the rapid reset of the sliding sleeve 408. Together with the impact ball 44, the stirring blade 410 can achieve reciprocating vibration in the horizontal direction during its rotation, thereby thoroughly agitating the microbial culture medium inside the treatment tank 401 and preventing the formation of sediment.

[0029] The separation assembly 3 includes a separation tank 301. Multiple annular grooves 302 are evenly spaced on the outer wall of the separation tank 301. A rotating rod 303 is fixedly connected to the central axis of the inner wall of the separation tank 301. The rotating rod 303 is rotatably connected to the inner wall of the housing 1. A pulley 304 is fixedly connected to one end of the rotating rod 303 that passes through the outer wall of the housing 1. A mounting base 305 is fixedly connected to the upper end of the housing 1. A motor 306 is fixedly connected to the upper end of the mounting base 305. A pulley 307 is fixedly connected to the output end of the motor 306. A drive belt 308 is installed between pulley 307 and pulley 304. The rotation of pulley 307, output by the motor 306, drives pulley 304 to rotate via the drive belt 308. Pulley 304 then drives the rotating rod 303 and the separation tank 301 to rotate, thereby separating the microorganisms in the separation tank 301.

[0030] The upper end of the separation tank 301 is open, and the outer wall of the separation tank 301 has multiple separation holes 3011, which are arranged in a circumferential array. The multiple separation holes 3011 enable the separation of microorganisms and improve the efficiency of the separation process.

[0031] The impact ball 44 is located on one side of the stirring blade 410, and the stirring blade 410 contacts the impact ball 44 when it rotates. After the stirring blade 410 contacts the impact ball 44, the reaction force will cause the sliding sleeve 408 to slide on the outer wall of the rotating rod 406.

[0032] The inner wall of the shell 1 has multiple annular grooves 5 corresponding to the first annular groove 302. Ball bearings 6 are movably disposed on the inner wall of the multiple annular grooves 5, and are movably connected to the first annular groove 302. The ball bearings 6 are located between the first annular groove 302 and the second annular grooves 5. The ball bearings 6 reduce vibration and noise during the centrifugation process of the separator 301, ensuring the stability of the separation process.

[0033] A bracket 7 is fixedly connected to the lower end of the shell 1. Switch valves are installed on the outer walls of the discharge pipe 2, the inlet 4011, and the connecting pipe 4014. An observation window 8, which is made of transparent glass, is installed on the outer wall of the shell 1. The bracket 7 improves the stability of the shell 1 during operation; the switch valves ensure the sealing of the shell 1 and the treatment tank 401, and can control the injection of the liquid, the backflow after treatment, and the discharge after separation; the observation window 8 allows the staff to observe the separation status inside the shell 1.

[0034] In practical use, this invention serves as a microbial separation device for microbial testing. The microbial culture medium to be separated is injected into the processing tank 401 through the inlet 4011. At this time, the motor 4013 is started, and the output shaft 405 rotates. The rotation of the shaft 405 drives the two rotating rods 406 to rotate. When the rotating rods 406 rotate, the gear 407 meshes and rolls along the tooth groove 404 at the upper end of the ring 403, thereby driving the rotating rods 406 to rotate. The rotation of the rotating rods 406 drives the sliding sleeve 408 and the stirring blade 410 to rotate synchronously, thus processing the microorganisms in the processing tank 401. The microbial culture medium is stirred, and as the stirring blade 410 rotates, the brush strip 411 contacts the bottom wall of the treatment tank 401, which can stir the microorganisms deposited at the bottom of the tank and reduce sedimentation. In addition, during the rotation of the stirring blade 410, it will come into contact with the impact ball 44. Under the action and reaction forces, the sliding sleeve 408 drives the stirring blade 410 to rotate and reciprocate along the horizontal direction of the rotating rod 406. Combined with the sweeping of the brush strip 411, the microbial culture medium in the treatment tank 401 is stirred without dead angles, further avoiding the sedimentation of microbial particles.

[0035] After pretreatment, open the switch valve on the connecting pipe 4014 to deliver the microbial culture medium to the separation tank 301, and start the motor 306 to drive the pulley 307 to rotate. The pulley 307 drives the pulley 304 and the rotating rod 303 to rotate through the drive belt 308, which in turn drives the separation tank 301 to rotate and generate centrifugal force. Under the action of centrifugal force, the microorganisms are thrown into the shell 1 through the separation hole 3011. After the separation tank 301 stops rotating, open the switch valve on the discharge pipe 2 to discharge the microorganisms inside the shell 1, thereby completing the separation of microorganisms.

[0036] Before use, connect the device to an external power source and a controller, which is a conventional, known device used for control.

[0037] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0038] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A microbial isolation device for microbial testing, comprising: The shell (1) is characterized in that: a discharge pipe (2) is fixedly connected to the lower end of the shell (1), a separation component (3) is provided on the inner wall of the shell (1), and a pretreatment component (4) is provided on the upper end of the shell (1). The pretreatment component (4) includes a treatment tank (401). Two mounting brackets (402) are fixedly connected to the outer wall of the treatment tank (401). The lower ends of the two mounting brackets (402) are fixedly connected to the upper end of the housing (1). A ring (403) is fixedly connected to the inner wall of the treatment tank (401). A toothed groove (404) is provided at the upper end of the ring (403). A shaft (405) is fixedly connected to the inner wall of the treatment tank (401). Two rotating rods (406) are rotatably connected to the outer wall of the shaft (405). Gears (407) are fixedly connected to the outer walls of the two rotating rods (406) at their opposite ends, and the two gears (407) are meshed with the tooth grooves (404). Two symmetrically distributed sliding sleeves (408) are slidably connected to the outer walls of the rotating rods (406). A connecting rod (409) is fixedly connected between the two sliding sleeves (408). A stirring blade (410) is fixedly connected to the outer walls of the sliding sleeves (408). A brush strip (411) is fixedly connected to the end of the stirring blade (410) away from the sliding sleeve (408).

2. The microbial isolation device for microbial testing according to claim 1, characterized in that: The upper end of the processing tank (401) is fixedly connected to the inlet (4011), the upper end of the processing tank (401) is fixedly connected to the frame (4012), the upper end of the frame (4012) is fixedly connected to the motor (4013), the output end of the motor (4013) is fixedly connected to the shaft (405) through a coupling, and the lower end of the processing tank (401) is fixedly connected to the connecting pipe (4014) that communicates with the shell (1).

3. The microbial isolation device for microbial testing according to claim 1, characterized in that: The pretreatment component (4) also includes a fixed sleeve (41) fixedly connected to the outer wall of the rotating rod (406). A return spring (42) is fixedly connected to one side of the fixed sleeve (41). The end of the return spring (42) away from the fixed sleeve (41) is fixedly connected to one of the sliding sleeves (408). Two connecting rods (43) are fixedly connected to the outer wall of the shaft (405). An impact ball (44) is fixedly connected to the ends of the two connecting rods (43) that are far apart from each other.

4. The microbial isolation device for microbial testing according to claim 1, characterized in that: The separation assembly (3) includes a separation tank (301). The outer wall of the separation tank (301) is provided with a plurality of annular grooves (302) at equal intervals. A rotating rod (303) is fixedly connected to the central axis of the inner wall of the separation tank (301). The rotating rod (303) is rotatably connected to the inner wall of the housing (1). A pulley (304) is fixedly connected to one end of the rotating rod (303) through the outer wall of the housing (1). A mounting base (305) is fixedly connected to the upper end of the housing (1). A motor (306) is fixedly connected to the upper end of the mounting base (305). A pulley (307) is fixedly connected to the output end of the motor (306). A drive belt (308) is installed between the pulley (307) and the pulley (304).

5. The microbial isolation device for microbial testing according to claim 4, characterized in that: The upper end of the separation tank (301) is open, and the outer side wall of the separation tank (301) is provided with a plurality of separation holes (3011), which are arranged in a circumferential array.

6. The microbial isolation device for microbial testing according to claim 3, characterized in that: The impact ball (44) is located on one side of the stirring blade (410), and the stirring blade (410) contacts the impact ball (44) when it rotates.

7. The microbial isolation device for microbial testing according to claim 4, characterized in that: The inner wall of the housing (1) is provided with a plurality of annular grooves (5) corresponding to annular groove one (302). The inner walls of the plurality of annular grooves (5) are provided with ball bearings (6), which are movably connected to annular groove one (302).

8. The microbial isolation device for microbial testing according to claim 2, characterized in that: The lower end of the housing (1) is fixedly connected to a bracket (7), and the outer walls of the discharge pipe (2), the inlet (4011) and the connecting pipe (4014) are all equipped with switch valves. The outer wall of the housing (1) is equipped with an observation window (8).

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