Composite microbial agent for promoting corn growth based on rhizosphere micro food web and application thereof

Abstract

The invention discloses a compound microbial agent for promoting corn growth based on rhizosphere micro food webs and application, and belongs to the technical field of agricultural microbial agents. The invention provides a compound microorganism bacterium agent for promoting growth and yield improvement of corn based on a rhizosphere micro food web. The compound microorganism bacterium agent is composed of bacterial strains of bacillus wiedmanni JN.2, bacterial strains of Flavobacterium ginossidimutans JN.3 and bacterial strains of Rosselomyces aquimaris L-3. The invention further provides a preparation method of the compound microorganism bacterium agent for promoting the growth and yield improvement of the corn based on the rhizosphere micro food web and a preparation method of the compound microorganism bacterium agent for promoting the growth and the yield improvement of the corn based on the rhizosphere micro food web and the preparation method of the compound microorganism bacterium agent for promoting the growth and the yield improvement of the corn based on the rhizosphere micro food web. By matching with a specific cultivation mode, namely, straw returning covering and sowing zone strip cultivation, the number of rhizosphere microbial populations can be remarkably increased, a rhizosphere micro food web can be directionally regulated and controlled, the soil structure is improved, nutrient release is accelerated, effective conversion of nutrients in the micro food web and transmission of the nutrients to corn plants are promoted, and corn growth and yield improvement are promoted; the method is environment-friendly and sustainable, convenient to use, simple to operate and high in economic benefit; the requirements on green and high-quality agricultural products are met.

Description

Technical Field

[0001] This invention relates to the field of agricultural microbial inoculants, and in particular to a compound microbial inoculant for promoting maize growth based on rhizosphere micro-food webs and its application. Background Technology

[0002] The rhizosphere is a micro-domain of soil environment strongly influenced by plant root activity, serving as the core interface for material exchange in the soil-plant system. The rhizosphere micro-food web, driven by root exudates, is a complex ecological network encompassing multiple trophic levels of organisms, including bacteria, fungi, protozoa, and nematodes. Through interactions such as predation and decomposition, this network dominates the mineralization and turnover of rhizosphere nutrients, playing a crucial regulatory role in crop nutrient acquisition, stress resistance, and growth and development. As a globally important food and forage crop, promoting high and stable yields of maize is of paramount importance.

[0003] However, under modern intensive farming models, the long-term monoculture system and excessive fertilizer input have led to an imbalance in the rhizosphere micro-ecosystem of maize. This manifests as a decline in soil biodiversity, a reduction in the number of key indicator species such as nematodes, and a degradation of microbial community function. Consequently, key ecological processes such as soil organic matter decomposition and nutrient cycling are hindered, resulting in a significant decrease in soil biofertility. This has become a potential bottleneck restricting further increases in maize yield and the green transformation of agriculture.

[0004] Currently, microbial technologies aimed at improving crop growth largely focus on developing single or compound microbial agents with direct growth-promoting functions (such as secreting growth hormones, fixing nitrogen, and solubilizing phosphorus). While these traditional microbial agents can improve crop growth to some extent, their mode of action is often limited to the direct pathway of "microbial agent-plant," and they generally suffer from unstable effects and short-lasting effects. The fundamental reason is that these methods neglect the synergistic interactions between organisms at various levels in the rhizosphere micro-food web, failing to integrate the functions of microbial agents into the broader soil ecological network, and thus unable to activate and maintain the "ecological engine" driving efficient nutrient cycling.

[0005] However, there is still a lack of mature and reliable technical solutions for constructing effective functional microbial agent combinations and systematically reconstructing and optimizing the rhizosphere micro-food web of maize through supporting agronomic measures to achieve stable and continuous promotion of maize growth. Therefore, developing a compound microbial agent that can precisely regulate the rhizosphere micro-food web to promote maize growth, increase yield, and optimize soil nutrient dynamics has significant theoretical importance and broad application prospects. Summary of the Invention

[0006] The purpose of this invention is to provide a compound microbial agent and its application for promoting maize growth based on the rhizosphere micro food web, in order to solve the problems of traditional microbial agents having single function, unstable effect and short duration of action, and to provide an effective solution for achieving green yield increase of crops through targeted regulation of the rhizosphere micro food web.

[0007] To achieve the above objectives, this invention provides a compound microbial agent for promoting maize growth based on rhizosphere micro-food webs, wherein the compound microbial agent is composed of... Bacillus wiedmannii JN.2、 Flavobacterium ginsenosidimutans JN.3 and Rossellomorea aquimaris Composition of L-3 strains.

[0008] Preferably, in the compound microbial agent Bacillus wiedmannii JN.2: Flavobacterium ginsenosidimutans JN.3: Rossellomorea aquimaris The bacterial viability ratio of L-3 is 1:1:1.

[0009] Preferably, the viable count of each of the three strains in the compound microbial agent is 1×10⁻⁶. 9 CFU / mL.

[0010] The application of the compound microbial agent for promoting maize growth based on the rhizosphere micro-food web, as described above, is an application in promoting maize growth and increasing maize yield.

[0011] Preferably, the method of application is to drench the roots after sowing.

[0012] Preferably, the bacterial solution is diluted before root irrigation, with a dilution ratio of 80-150 times.

[0013] Preferably, the seeds are sown in fields where straw is laid in rows and the sowing strips are tilled.

[0014] Preferably, the root irrigation volume is 30L of compound microbial agent diluted solution per acre.

[0015] Bacillus wiedmannii JN.2, classified as Bacillus wiedmannii JN.2 was deposited at the China Center for Type Culture Collection on December 17, 2025, with accession number CCTCC NO: M 20252922. Flavobacterium ginsenosidimutans JN.3, classified as Flavobacterium ginsenosidimutans JN.3 was deposited at the China Center for Type Culture Collection on December 17, 2025, with accession number CCTCC NO: M 20252923. Rossellomorea aquimaris L-3, classified as Rossellomorea aquimarisL-3 was deposited at the China Center for Type Culture Collection on July 22, 2024, with accession number CCTCC NO: M 20241623.

[0016] Therefore, the specific technical effects of the compound microbial agent for promoting maize growth based on the rhizosphere micro food web and its application provided by this invention are as follows: (1) This invention provides a compound microbial agent for promoting maize growth and yield based on rhizosphere micro-food web, which is composed of... Bacillus wiedmannii JN.2、 Flavobacterium ginsenosidimutans JN.3 and Rossellomorea aquimaris Composition of L-3 strains; combined with specific tillage patterns—straw row mulching and strip tillage for sowing—can significantly increase the number of rhizosphere microbial communities, directionally regulate the rhizosphere micro-food web, improve soil structure, and promote maize growth and yield; (2) The compound microbial agent provided by the present invention, combined with the tillage mode of straw row covering and strip sowing, can accelerate nutrient release, activate the rhizosphere micro food web, promote the effective transformation of nutrients in the micro food web and the transfer to corn plants; it is environmentally friendly and sustainable, only requires one application, and can play a continuous role throughout the entire growth period of corn. It is convenient to use, simple to operate, and has high economic benefits. (3) The strains in the compound microbial agent provided by the present invention are all bacteria that exist in the soil itself, and have high safety; it not only reduces the potential negative impact of chemical input on soil and ecological environment, but also helps to build a long-term healthy soil environment by improving the soil micro-ecological balance, and has good potential for sustainable development; it meets the requirements for green and high-quality agricultural products.

[0017] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments of the present invention will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is the evolutionary tree drawn in Embodiment 1 of the present invention; where a is Bacillus wiedmannii JN.2 strain; b is... Flavobacterium ginsenosidimutans JN.3 strain; c is... Rossellomorea aquimaris L-3 strain; Figure 2 This is a schematic diagram of the experimental and control group cell layout in Embodiment 2 of the present invention; where blue represents the experimental group and yellow represents the control group; Figure 3 This is the statistical result of the number of soil nematodes in the rhizosphere soil samples of maize collected at the maize maturity stage in Example 2 of this invention; wherein express P <0.01; Figure 4 This is the statistical result of soil nematode species in the rhizosphere soil samples collected during the corn maturity period in Example 2 of the present invention; Figure 5 This is a statistical result of the maize plant height at each treatment during the maize maturity stage in Example 2 of the present invention; wherein express P <0.05; Figure 6 These are the statistical results of corn ear height at different treatments during the corn maturity stage in Example 2 of this invention; wherein express P <0.05; Figure 7 This is a statistical result of the corn stalk diameter at different treatments during the corn maturity stage in Example 2 of the present invention; wherein express P <0.05; Figure 8 This refers to the statistical results of the maize leaf area index for each treatment at the maize maturity stage in Example 2 of the present invention; wherein... express P <0.05; Figure 9 These are the statistical results of soil carbon component content in various treatments during the maize maturity stage in Example 2 of this invention; where a is SOC; b is DOC; c is POC; and d is MAOC. express P <0.05, express P <0.01, express P <0.0001; Figure 10 This refers to the statistical results of maize yield at various treatments during the maize maturity stage in Example 2 of the present invention; wherein... express P <0.01. Detailed Implementation

[0020] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0021] To make the objectives, technical solutions, and advantages of this application clearer, more thorough, and more complete, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings and embodiments. The following detailed descriptions are all illustrations of embodiments, intended to provide further detailed explanation of the present invention. Unless otherwise specified, all technical terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0022] The instruments, equipment, reagents and materials used in the embodiments were all obtained through commercial means; the methods and steps not described in detail in the embodiments are all conventional techniques in the art.

[0023] The culture media and reagents used in the examples are as follows: Enrichment medium: NaNO3 0.5g, KCl 0.5g, KH2PO4 1.0g, Fe2(SO4)3·7H2O (prepare a 1% stock solution first, then take 1mL), MgSO4·7H2O 0.5g, corn stalk powder (crush the corn stalks and pass them through a 60-mesh sieve) 10g, add distilled water to 1L, and sterilize in an autoclave at 121°C for 20min; Isolation medium (NA medium): 3.0g beef extract, 10.0g peptone, 5.0g NaCl, 18.0g agar, 1L distilled water, pH 7.0~7.2, sterilized in an autoclave at 121°C for 20min; Differential medium (carboxymethyl cellulose sodium medium, CMC-Na): CMC-Na 20.0g, (NH4)2SO4 2.0g, MgSO4·7H2O 0.5g, KH2PO4 1g, NaCl 0.5g, distilled water 1L, agar 20g, pH 7.0~7.2, autoclaved at 121°C for 20min; LB medium: 10g tryptone, 5g NaCl, 5g yeast extract, 1L distilled water, sterilized in an autoclave at 121°C for 20min; NGM solid culture medium: 2.5g peptone, 3.0g sodium chloride, 17.0g agar, 1L distilled water. Sterilize in an autoclave at 121°C for 20min. After the solution temperature drops to 65°C, in a clean bench, filter through a sterile membrane and add 25mL phosphate buffer, 1mL 1mol / L magnesium sulfate solution, 1mL 1mol / L calcium chloride solution, and 1mL 5g / L cholesterol anhydrous ethanol solution. Mix well and pour into sterile petri dishes. TSB solid culture medium: 15g tryptic powder, 5g NaCl, 5g soybean peptone, 15g agar powder, 1L distilled water. Sterilize in an autoclave at 121°C for 20 minutes. After the solution temperature drops to 65°C, pour it into sterile petri dishes in a laminar flow hood.

[0024] Reagents used: 0.1% Congo Red dye solution: Weigh 0.1g of Congo red dye using a balance, add distilled water and stir well, then bring the volume to 100mL. 1 mol / L sodium chloride solution: Weigh 58.44 g of sodium chloride using a balance, add distilled water and stir well, then bring the volume to 1 L; Phosphate buffer: Dissolve 3.56 g of dipotassium hydrogen phosphate and 10.83 g of potassium dihydrogen phosphate in 100 mL of sterile distilled water; Magnesium sulfate solution: 12.03 g of anhydrous magnesium sulfate reagent is dissolved in 100 mL of sterile distilled water; Calcium chloride solution: Dissolve 11.38 g of anhydrous calcium chloride reagent in 100 mL of sterile distilled water; Cholesterol solution: 5 mg of cholesterol is dissolved in 1 mL of anhydrous ethanol; Nematode surface disinfectant: Mix sodium hypochlorite solution and 5 mol / L sodium hydroxide solution at a volume ratio of 2:1, and dilute 10 times; M9 buffer solution: 5g sodium chloride, 3g potassium dihydrogen phosphate, 6g disodium hydrogen phosphate, add distilled water to 1L, autoclave at 121°C for 20min, then filter through a sterile membrane and add 1mL of 1mol / L magnesium sulfate solution.

[0025] The strain information used is as follows: Bacillus wiedmannii JN.2 was deposited at the China Center for Type Culture Collection on December 17, 2025, with accession number CCTCC NO: M 20252922; Flavobacterium ginsenosidimutans JN.3 was deposited at the China Center for Type Culture Collection on December 17, 2025, with accession number CCTCC NO: M 20252923; Rossellomorea aquimaris L-3 was deposited at the China Center for Type Culture Collection on July 22, 2024, with accession number CCTCC NO: M 20241623.

[0026] Example 1 The isolation and identification of the strains are detailed below: Screening of S1, cellulose-degrading bacteria JN.2 and JN.3.

[0027] A: Isolation of cellulose-degrading bacteria.

[0028] Weigh 10g of soil sample collected from the Changtu Modern Agricultural Experiment Station of the Shenyang Institute of Applied Ecology, Chinese Academy of Sciences, Tieling City, Liaoning Province, and place it in 90mL of sterile water containing glass beads. Shake at 150r / min for 1h, then let it stand for 5min. Take 2mL of the supernatant and place it in sterile and cooled enrichment medium, and incubate at 20°C with shaking at 180r / min for 3d. Serially dilute the enriched culture medium, and take 10g of the supernatant. -6 10 -7 10 -8 0.1 mL of the diluted solution was spread onto NA medium for separation. Three parallel groups were set up for each gradient, and the culture was inverted at 30°C for 1 to 2 days.

[0029] B: Purification of cellulose-degrading bacteria.

[0030] After plate culture, representative colonies were selected based on characteristics such as colony quantity, morphology, and color. Colonies were isolated on NA medium using a sterile inoculating loop until purified single colonies were obtained. These single colonies were then stored in LB medium and allowed to grow sufficiently before being kept at 4°C for later use.

[0031] C: Screening of cellulose-degrading bacteria.

[0032] The purified strains were inoculated onto CMC-Na agar plates using an inoculation loop. After incubation at 30°C, strains capable of growing on the plates were selected and stained using the Congo red clear zone staining method. Specifically, the identification medium plates were stained with 0.1% Congo red for 20 min, followed by destaining with 1 mol / L sodium chloride solution for 30 min. Excess solution was discarded from the plates. Finally, the dominant cellulose-degrading bacteria were screened based on the ratio of the diameter of the clear zone (D) to the colony diameter (d) on the medium plates. The D / d values ​​were calculated, and the D / d values ​​for strains JN-2 and JN-3 at 30°C were 2.1–2.57 and 3.5–2.73, respectively.

[0033] D: Strains are preserved.

[0034] The obtained JN.2 strain was identified as belonging to... Bacillus wiedmannii The obtained JN.2 strain was deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 20252922.

[0035] The obtained JN.3 strain was identified as belonging to... Flavobacterium ginsenosidimutans The obtained JN.3 strain was deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 20252923.

[0036] S2, screening of nematode intestinal strain L-3.

[0037] A: Nematode surface disinfection and washing. First, disinfect the surface of the nematodes (Nematodes of the genus *C. nigra*) grown on NGM solid medium. Transfer the nematodes to a 1.5 mL centrifuge tube, add 1 mL of sterile distilled water, and wash. Centrifuge at 3000 rpm for 1 minute, remove the supernatant, and retain approximately 0.1 mL of liquid in the centrifuge tube. Repeat this step three times, adding 1 mL of sterile distilled water each time. After washing, add 200 μL of M9 buffer and 400 μL of nematode surface disinfection solution to the remaining 0.1 mL of liquid in the centrifuge tube, mix well, and let stand for 2 minutes. Then, centrifuge at 3000 rpm for 1 minute, discard the supernatant, and wash the precipitate three more times with M9 buffer.

[0038] B: Nematode Grinding and Colony Culture. After step A, water control treatment is performed. Then, the nematodes frozen in liquid nitrogen are ground into powder. Take 5 mg of the powder and immediately add 1 mL of sterile distilled water, vortex to mix for 30 seconds. After standing for 10 minutes, take the supernatant and dilute it on TSB solid medium for plating. Incubate for 24 hours to obtain various single colonies.

[0039] C: Colony purification. The single colonies obtained in step B are inoculated onto fresh TSB solid medium using an inoculation loop. If different colonies still grow on the new medium, continue streaking until only one type of colony appears on each medium, thus completing colony purification.

[0040] D: Strains are preserved.

[0041] The L-3 strain was identified as belonging to Rossellomorea aquimaris The L-3 strain was deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 20241623.

[0042] The phylogenetic trees of strains JN.2, JN.3, and L-3 are as follows: Figure 1 As shown.

[0043] Example 2 The specific steps for developing and applying a compound microbial agent that promotes maize growth based on the rhizosphere microfood web are as follows: S1. Fermentation and application of compound microbial agents.

[0044] A: respectively Bacillus wiedmannii JN.2、 Flavobacterium ginsenosidimutans JN.3 and Rossellomorea aquimaris L-3 strain was fermented at a fermentation company, and the three strains were fermented to approximately 1×10⁻⁶. 9 CFU / mL level.

[0045] B: The received fermented product Bacillus wiedmannii JN.2、 Flavobacteriumginsenosidimutans JN.3 and Rossellomorea aquimaris The L-3 strain was mixed in a volume ratio of 1:1:1 to obtain a compound bacterial agent.

[0046] C: Mix the compound bacterial agent with water at a volume ratio of 1:100 to obtain the compound bacterial working solution, which is used for root irrigation during the corn sowing period.

[0047] S2. At the Changtu Modern Agricultural Experiment Station of the Shenyang Institute of Applied Ecology, Chinese Academy of Sciences, Tieling City, Liaoning Province, a cornfield with straw mulching and strip tillage was selected as the experimental site.

[0048] S3. Set up two treatments: an experimental group and a control group. Each treatment has three replicates. Each replicate experimental plot is 5m wide and 5.3m long. The experimental group plots and control group plots are spaced apart and located on the same tillage strip (e.g., ...). Figure 2 (As shown). On May 5, 2025, corn was sown using a no-till corn planter. The corn variety was Xianyu 1483. A straw-covered strip tillage and two-way open-field planting pattern was adopted, with an average planting density of 4200 plants / mu. Fertilization was performed using a nitrogen-phosphorus-potassium compound fertilizer as base fertilizer, applied once at a rate of 60 kg / mu. The compound fertilizer contained N, P2O5, and K2O in a ratio of 26:10:12. Immediately after corn sowing, the experimental group was irrigated with the compound bacterial solution prepared in step S1 (C) at a rate of 30 L / mu. The control group was immediately irrigated with an equal amount of water after corn sowing. Both the experimental and control groups were managed conventionally until the corn reached maturity.

[0049] S4. The number and species of nematodes in the rhizosphere soil, various growth indicators of maize (plant height, ear height, stem diameter, leaf area index), soil carbon content and yield are key indicators for measuring the regulatory effect of the rhizosphere micro food web. They can effectively characterize the impact of compound microbial agents on the rhizosphere micro food web and the coupling relationship between this impact and maize growth.

[0050] After all treatments were sown (the day was recorded as day 1 of the experiment), sampling and measurements were taken at the maturity stage of maize (day 135 of the experiment). Whole maize plants were collected for growth index determination, and soil samples from the rhizosphere (0-20 cm) were collected for soil nematode isolation. Soil samples from 9 plants were measured for each index. ① Isolation and counting of nematodes: 100g of fresh soil sample was taken, and soil nematodes were isolated and extracted using a modified shallow dish method, and counted under a microscope. The samples were then sent to the company for nematode species identification.

[0051] ② Corn plant height: Measure and record the height of the collected corn plants using a measuring tape.

[0052] ③ Ear height of corn: Measure the ear height of the collected corn plants with a measuring tape and record it.

[0053] ④ Corn stem diameter: Measure the stem diameter of the collected corn plants from the first node at the base of the plant using a tape measure and record the measurement.

[0054] ⑤ Maize Leaf Area Index: Measure the length and width of the first leaf below the ear of the collected maize plant with a tape measure and record the measurements. Calculate the leaf area index using the following formula.

[0055] Leaf area per plant (cm²) 2 = Leaf length (cm) × Leaf width (cm) × 0.75; Leaf Area Index (LAI) = Leaf area per plant × Number of plants in a plot / Plot area.

[0056] ⑥ Soil carbon composition content: The total organic carbon (SOC) content of soil was determined by an elemental analyzer; the soluble organic carbon (DOC) content was determined by water extraction-TOC analysis; the particulate organic carbon (POC) content was determined by potassium permanganate oxidation method; and the mineral-bound organic carbon (MAOC) was separated by density separation-chemical oxidation method, and then the MAOC carbon content was determined by an elemental analyzer.

[0057] ⑦ Maize Yield: Each model yield measurement field was divided into 10 plots, and 3 plots were randomly selected as sampling points. Each sampling point was >12.0m. 2 Fresh ears of corn were harvested manually from sample sites, and their fresh weight was measured. Ten standard ears were taken from each sample site, and their fresh weight and grain weight were measured to calculate the kernel yield. The moisture content was measured using an Aipu LDS-5G grain moisture meter, repeated three times, and the average value was taken. The standard moisture content for corn was calculated as 14%.

[0058] Shell yield = (fresh weight of grains / fresh weight of whole ear) × 100%; Actual yield (kg / mu) = [(actual fresh weight of harvested ears / actual harvested area) × 666.7 × shelling rate × (1 - moisture content)] (1 - 14%).

[0059] Results of rhizosphere soil nematode count measurements as follows Figure 3 As shown, compared with the control group, the number of soil nematodes in the group inoculated with compound microbial agents was significantly increased, indicating that the microbial agents significantly affected the soil micro food web.

[0060] Soil nematode species identification results as follows Figure 4 As shown, treatment with compound microbial inoculants can effectively regulate the structure of soil nematode communities. This change may be related to the mechanism of action of the microbial inoculants, indicating that microbial inoculants can affect the function and structure of different nematode communities in the soil ecosystem by improving the soil micro-food web. The results for maize growth status are shown below. Figures 5 - 8As shown, compared to the blank control group, the maize plant height, ear height, stem diameter, and leaf area index of the group inoculated with the compound microbial agent were significantly increased, indicating that the compound microbial agent can effectively promote maize growth. These indicators are commonly used to evaluate plant growth status, and their improvement reflects the enhanced overall growth vigor of maize plants.

[0061] Statistical results of soil carbon content are as follows: Figure 9 As shown, inoculation with compound microbial agents increased the content of several types of carbon (SOC, DOC, POC, MAOC) in the soil, especially the most significant increase in dissolved organic carbon (DOC). This indicates that compound microbial agents help enhance the transformation and release of soil organic carbon.

[0062] The statistical results of corn yield are as follows: Figure 10 As shown, the use of compound microbial inoculants helped increase maize yield, significantly better than the control group that did not use inoculants. This indicates that compound microbial inoculants have the potential to promote crop growth and yield in agricultural production, possibly by enhancing soil fertility and plant growth conditions through promoting root micro-food webs and improving soil carbon cycling.

[0063] Therefore, this invention provides a compound microbial agent based on rhizosphere microfood web to promote maize growth and yield, consisting of... Bacillus wiedmannii JN.2、 Flavobacterium ginsenosidimutans JN.3 and Rossellomorea aquimaris Composed of L-3; when combined with specific tillage patterns—straw row mulching and strip tillage—it can significantly increase the number of rhizosphere microbial communities, directionally regulate the rhizosphere micro-food web, improve soil structure, accelerate nutrient release, promote the effective transformation of nutrients in the micro-food web and their transfer to maize plants, and promote maize growth and yield; it is environmentally friendly and sustainable, easy to use, simple to operate, and has high economic benefits; it meets the requirements for green and high-quality agricultural products.

[0064] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A compound microbial inoculant for promoting maize growth based on rhizosphere micro-food webs, characterized in that: The compound microbial agent is composed of Bacillus wiedmannii JN.2、 Flavobacterium ginsenosidimutans JN.3 and Rossellomorea aquimaris L-3 strain composition; Bacillus wiedmannii JN.2, classified as Bacillus wiedmannii JN.2 was deposited at the China Center for Type Culture Collection on December 17, 2025, with accession number CCTCC NO: M 20252922; Flavobacterium ginsenosidimutans JN.3, classified as Flavobacterium ginsenosidimutans JN.3 was deposited at the China Center for Type Culture Collection on December 17, 2025, with accession number CCTCC NO: M 20252923; Rossellomorea aquimaris L-3, classified as Rossellomorea aquimaris L-3 was deposited at the China Center for Type Culture Collection on July 22, 2024, with accession number CCTCC NO: M 20241623.

2. The compound microbial agent for promoting maize growth based on rhizosphere microfood web as described in claim 1, characterized in that: The compound microbial agent Bacillus wiedmannii JN.2: Flavobacterium ginsenosidimutans JN.3: Rossellomorea aquimaris The bacterial viability ratio of L-3 is 1:1:

1.

3. The compound microbial agent for promoting maize growth based on rhizosphere microfood web as described in claim 1, characterized in that: The viable count of the three strains in the compound microbial agent is 1×10⁻⁶. 9 CFU / mL.

4. The application of the compound microbial agent for promoting maize growth based on rhizosphere microfood web as described in any one of claims 1-3, characterized in that: The application is in promoting corn growth and increasing corn yield.

5. The application of the compound microbial agent for promoting maize growth based on rhizosphere microfood web as described in claim 4, characterized in that: The method of application is to drench the roots after sowing.

6. The application of the compound microbial agent for promoting maize growth based on rhizosphere micro-food web as described in claim 5, characterized in that: Dilute the bacterial solution before root irrigation, with a dilution ratio of 80-150 times.

7. The application of the compound microbial agent for promoting maize growth based on rhizosphere micro-food web as described in claim 5, characterized in that: Seeds are sown in fields where straw is laid in rows and the sowing strips are tilled.

8. The application of the compound microbial agent for promoting maize growth based on rhizosphere microfood web as described in claim 6, characterized in that: The root irrigation volume is 30L of diluted compound microbial agent per acre.

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