Blood sample refrigeration transfer box with temperature compensation function and sample state real-time monitoring method

Abstract

The invention relates to a blood sample cold storage transfer box with a temperature compensation function and a sample state real-time monitoring method, and belongs to the technical field of medical instruments and cold-chain logistics. The blood sample cold storage transfer box comprises a box body, a box cover, a heat preservation inner container, a phase change cold storage layer, a semiconductor chilling plate, a microcontroller, a temperature sensor and a sample state monitoring unit; the sample state monitoring unit comprises a light reflection type hemolysis detection module and an anti-leakage sensing layer. According to the invention, the microcontroller actively controls the semiconductor chilling plate to carry out refrigeration or heating compensation according to the feedback of the temperature sensor, and precise regulation and control of the temperature in the box are realized; meanwhile, the hemolysis state of the sample is monitored in a non-contact mode through an optical means, liquid leakage is monitored in real time through the conductive grid, the quality and biological safety of the blood sample in the transferring process are effectively guaranteed, and the whole-process traceability of the sample state is achieved.

Description

Technical Field

[0001] This invention relates to the fields of medical devices and cold chain logistics technology, and in particular to a blood sample refrigerated transport box with temperature compensation function and a method for real-time monitoring of sample status. Background Technology

[0002] The transport of blood samples from collection to testing is a critical step in clinical laboratory quality control. Blood contains highly temperature-sensitive cellular components, enzymes, proteins, and other bioactive substances. Traditional blood sample transport primarily relies on passive refrigerated boxes, which maintain a low-temperature environment using pre-cooled ice packs (phase change materials) and insulation. However, this approach has significant drawbacks: First, during prolonged transport or in extremely high / low ambient temperatures, the ice packs' cooling capacity may be depleted or released too quickly, causing the box temperature to exceed the standard range of 2℃-8℃, resulting in hemolysis of the sample or distorted test results. Second, existing transport boxes lack sample status sensing capabilities. When sample tubes rupture and leak, or when hemolysis occurs due to bumps or temperature fluctuations during transport, these issues cannot be detected promptly, leading to substandard samples entering the testing process, wasting medical resources and potentially delaying diagnosis.

[0003] Although some cold chain boxes with temperature recording functions are available on the market, they are limited to post-event traceability and lack active temperature control methods. Furthermore, they cannot monitor the physical state of the sample itself (hemolysis, leakage) in real time. Therefore, developing an intelligent transport box that can actively compensate for temperature and monitor the sample status in real time has important clinical application value. Summary of the Invention

[0004] This invention provides a blood sample refrigerated transport box with temperature compensation function and a method for real-time monitoring of sample status, in order to solve the technical problems of existing blood sample transport boxes failing to control temperature during long-term transportation or under extreme environments, being unable to actively adjust temperature, and being unable to monitor the hemolysis and leakage status of samples in real time.

[0005] The present invention provides the following solution to the above-mentioned technical problems: a blood sample cold storage transport box with temperature compensation function and a method for real-time monitoring of sample status, comprising a box body, a box cover, a phase change cold storage layer, a semiconductor refrigeration chip, a microcontroller, a temperature sensor, a sample status monitoring unit, and a sample tube, characterized in that: the box body is provided with an insulated inner liner;

[0006] The phase change cold storage layer is disposed between the outer wall of the heat-insulating inner liner and the inner wall of the box.

[0007] The cold end of the semiconductor cooling chip is fitted to the outer wall of the heat-insulating inner liner, and its hot end is connected to a heat dissipation device.

[0008] The microcontroller is housed in a compartment within the housing.

[0009] The temperature sensor is installed on the inner wall of the heat-insulating liner and is electrically connected to the microcontroller.

[0010] The sample status monitoring unit includes a light-reflective hemolysis detection module and a leak-proof sensing layer;

[0011] The light-reflective hemolysis detection module is located inside the box cover and is used to emit detection light to the sample tube and receive reflected light.

[0012] The leak-proof sensing layer is laid at the bottom of the heat-insulating inner liner and consists of a spaced conductive mesh.

[0013] The microcontroller is electrically connected to the semiconductor cooling chip, the temperature sensor, the light-reflective hemolysis detection module, and the leak-proof sensing layer, and is configured to: when the temperature detected by the temperature sensor is higher than a first preset threshold or lower than a second preset threshold, activate the semiconductor cooling chip to perform cooling or heating compensation.

[0014] Based on the above technical solution, the present invention can be further improved as follows.

[0015] Furthermore, the light-reflection hemolysis detection module includes a light source and a spectral sensor. The light source emits visible light with a wavelength range of 500nm-700nm, and the spectral sensor is used to receive the reflection spectrum of the blood sample in the sample tube. The microcontroller determines the degree of hemolysis based on the intensity change of the characteristic absorption peak of hemoglobin in the reflection spectrum.

[0016] Furthermore, the leak-proof sensing layer includes two parallel flexible conductive films and an insulating mesh disposed between the two flexible conductive films. When liquid seeps in, the two flexible conductive films become conductive, and the microcontroller detects the decrease in resistance and generates a leak alarm signal.

[0017] Furthermore, it also includes a wireless communication module, which is electrically connected to the microcontroller and is used to upload temperature data, hemolysis status data, and leakage alarm data to the remote monitoring platform in real time.

[0018] Furthermore, the heat dissipation device includes heat dissipation fins and a micro fan, the heat dissipation fins being attached to the hot end of the semiconductor cooling chip, and the micro fan being positioned towards the heat dissipation fins.

[0019] On the other hand, a method for real-time monitoring of sample status in a transport box includes the following steps:

[0020] S1 collects the temperature inside the chamber in real time through a temperature sensor. When the temperature deviates from the preset range of 2℃-8℃, the microcontroller controls the semiconductor cooling chip to start and perform cooling or heating temperature compensation.

[0021] S2, at a preset time point during the transport process or when triggered by the user, the light reflection hemolysis detection module emits detection light into the sample tube, collects the reflection spectrum, and the microcontroller calculates the hemolysis index. If the hemolysis index exceeds the preset threshold, a hemolysis alarm is generated.

[0022] S3, the leak-proof sensing layer monitors the resistance value between the conductive meshes in real time. When the resistance value is lower than the preset resistance threshold, it determines that a liquid leak has occurred and generates a leak alarm.

[0023] S4, the microcontroller sends temperature data, hemolysis alarm information and leakage alarm information to the remote monitoring terminal through the wireless communication module.

[0024] Furthermore, in step S2, the hemolysis index is calculated by extracting the ratio of absorbance at dual wavelengths of 540nm and 576nm from the reflectance spectrum and comparing it with the curve of a standard hemolysis sample.

[0025] The beneficial effects of this invention are as follows: This invention provides a blood sample refrigerated transport box with temperature compensation function and a method for real-time monitoring of sample status, which has the following advantages:

[0026] 1. Active temperature compensation: By setting up a semiconductor refrigeration chip and a phase change cold storage layer to work together, active cooling / heating capacity is added on the basis of passive cold storage of phase change materials. When the temperature inside the box deviates from the set range, the microcontroller can activate the semiconductor refrigeration chip in real time to compensate, which significantly extends the effective temperature control time and adapts to more complex transportation environments.

[0027] 2. Real-time sample status sensing: It integrates a non-contact optical hemolysis detection module, which can quickly determine whether the sample is hemolyzed without opening the cap, preventing unqualified samples from entering the testing process; at the same time, the leak-proof sensing layer at the bottom can detect liquid leakage caused by sample tube rupture in the first instance, ensuring biosafety.

[0028] 3. Full data traceability: Through the wireless communication module, all temperature data and alarm information are uploaded to the monitoring platform in real time, realizing transparent and intelligent management of the transfer process and meeting the information needs of pre-inspection quality control.

[0029] The above description is merely an overview of the technical solution of the present invention. In order to better understand the technical means of the present invention and to implement it according to the contents of the specification, the preferred embodiments of the present invention are described in detail below with reference to the accompanying drawings. Specific embodiments of the present invention are given in detail below with reference to the accompanying drawings. Attached Figure Description

[0030] The accompanying drawings, which are included to provide a further understanding of the invention and form part of this application, illustrate exemplary embodiments of the invention and, together with their description, serve to explain the invention and do not constitute an undue limitation thereof. In the drawings:

[0031] Figure 1 This is a schematic diagram of the structure of a blood sample refrigerated transport box with temperature compensation function and a real-time monitoring method for sample status provided in an embodiment of the present invention;

[0032] Figure 2 This is a schematic diagram of the structure of a blood sample refrigerated transport box with temperature compensation function and a method for real-time monitoring of sample status provided in an embodiment of the present invention;

[0033] Figure 3 A three-dimensional rendering of a blood sample refrigerated transport box with temperature compensation function and a method for real-time monitoring of sample status provided in an embodiment of the present invention;

[0034] Figure 4 This invention provides a system architecture diagram of a blood sample refrigerated transport box with temperature compensation function and a method for real-time monitoring of sample status, according to an embodiment of the present invention.

[0035] Figure 5 The present invention provides a method flowchart for a blood sample refrigerated transport box with temperature compensation function and a method for real-time monitoring of sample status, as an embodiment of the present invention.

[0036] The attached diagram lists the components represented by each number as follows:

[0037] 1. Box body; 101. Flexible conductive film; 102. Insulating mesh; 2. Box cover; 3. Insulated inner liner; 4. Phase change cold storage layer; 5. Semiconductor cooling chip; 6. Microcontroller; 7. Temperature sensor; 8. Optical reflection hemolysis detection module; 801. Light source; 802. Spectral sensor; 9. Sample tube; 10. Leak-proof sensing layer; 11. Wireless communication module; 12. Heat sink fins; 13. Miniature fan. Detailed Implementation

[0038] The following is in conjunction with the appendix Figure 1-5 The principles and features of the present invention are described below. The examples given are for illustrative purposes only and are not intended to limit the scope of the invention. The invention is described more specifically in the following paragraphs by way of example with reference to the accompanying drawings. The advantages and features of the invention will become clearer from the following description. It should be noted that the drawings are in a very simplified form and use non-precise proportions, and are only used to facilitate and clarify the illustration of the embodiments of the invention.

[0039] It should be noted that when a component is described as "fixed to" another component, it can be directly on the other component or may have a component in between. When a component is considered "connected to" another component, it can be directly connected to the other component or may have a component in between. When a component is considered "set on" another component, it can be directly set on the other component or may have a component in between. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0040] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0041] like Figure 1-3 As shown, the present invention provides a blood sample refrigerated transport box with temperature compensation function, including a box body 1 and a box cover 2 hinged to the box body 1;

[0042] The box 1 is equipped with an insulated inner liner 3, which is made of a high thermal conductivity material (such as aluminum alloy) and is used to accommodate the sample tube 9. A sandwich space is formed between the box 1 and the insulated inner liner 3. A phase change cold storage layer 4 is provided between the outer wall of the insulated inner liner 3 and the inner wall of the box 1. In this embodiment, the phase change cold storage layer 4 is made of a paraffin-based composite material with a melting point of 4°C. It is uniformly covered on the outer periphery of the insulated inner liner 3 and is used to maintain the basic cold amount at room temperature, thus playing a passive insulation role.

[0043] In the interlayer of the box body 1, a semiconductor cooling chip 5 is attached to one side of the outer wall of the heat-insulating inner liner 3. Specifically, the cold end of the semiconductor cooling chip 5 is tightly attached to the outer wall of the heat-insulating inner liner 3 by thermally conductive silicone grease, and its hot end is connected to a heat dissipation device. The heat dissipation device includes heat dissipation fins 12 and a micro fan 13. The heat dissipation fins 12 are attached to the hot end of the semiconductor cooling chip 5, and the micro fan 13 is positioned towards the heat dissipation fins 12 for forced heat dissipation and to improve heat exchange efficiency.

[0044] The microcontroller 6 is located in the interlayer of the housing 1 and is electrically connected to the semiconductor cooling chip 5. The temperature sensor 7 is located on the inner wall of the heat-insulating inner liner 3 and is electrically connected to the microcontroller 6. It is used to collect the temperature inside the housing in real time. The wireless communication module 11 is also located in the interlayer of the housing 1 and is electrically connected to the microcontroller 6. It is used to interact with the remote monitoring platform.

[0045] Example 2: Sample Status Monitoring Unit, please continue reading Figure 1-4The sample status monitoring unit of the present invention includes a light-reflective hemolysis detection module 8 and a leak-proof sensing layer 10;

[0046] The light reflection hemolysis detection module 8 is located inside the lid 2, corresponding to the opening of the sample tube 9. This module includes a light source 801 and a spectral sensor 802. The light source 801 is an LED light source that can emit visible light with a wavelength range of 500nm-700nm. The spectral sensor 802 is used to receive the reflection spectrum of the blood sample in the sample tube 9. The microcontroller 6 determines the degree of hemolysis based on the intensity change of the characteristic absorption peak of hemoglobin in the reflection spectrum. This non-contact detection method can be completed quickly without opening the lid, avoiding cross-contamination.

[0047] Leak-proof sensing layer 10 is laid at the bottom of the insulation inner liner 3, such as... Figure 2 As shown in the enlarged view, the leak-proof sensing layer 10 includes two parallel flexible conductive films 101 and an insulating mesh 102 disposed between the two flexible conductive films 101. In this embodiment, the flexible conductive film 101 is a PET-ITO conductive film, and the insulating mesh 102 is a nylon mesh. Under normal conditions, the resistance between the two flexible conductive films 101 is infinite. When liquid seeps in, the two flexible conductive films 101 become conductive, and the microcontroller 6 detects the decrease in resistance and generates a leak alarm signal.

[0048] To prevent false alarms from the leak-proof sensor layer 10 due to condensation buildup inside the box, this embodiment also includes the following anti-interference mechanism:

[0049] The microcontroller 6 is configured such that when the resistance value between the two flexible conductive films 101 is detected to be lower than a preset resistance threshold, it does not immediately generate a leakage alarm signal, but instead initiates a delay judgment program. During the delay period, for example, 10 seconds, if the resistance value remains below the threshold and shows no trend of recovery, and in conjunction with whether the current temperature detected by the temperature sensor 7 is lower than the dew point temperature, a sample leakage is determined only when both conditions are met.

[0050] In addition, a layer of hydrophilic non-woven fabric is attached to the bottom of the leak-proof sensing layer 10 to quickly absorb the leaked liquid, ensuring that the liquid can quickly conduct the two flexible conductive films 101, improving the detection sensitivity, and avoiding the problem of multiple points of non-conduction due to liquid rolling.

[0051] To ensure that the optical reflection hemolysis detection module 8 accurately acquires the reflection spectrum under non-contact conditions, this embodiment further optimizes its optical structure. The optical reflection hemolysis detection module 8 also includes a coaxial optical path assembly, specifically:

[0052] The light source 801 employs a ring-shaped LED array, arranged around the spectral sensor 802, with the central axis of the ring-shaped LED array coinciding with the axis of the sample tube 9's opening. A collimating lens group is positioned between the light source 801 and the spectral sensor 802 to focus the emitted light onto the central region of the blood sample surface within the sample tube 9. An elastic light shield is positioned between the inside of the lid 2 and the light-reflective hemolysis detection module 8. When the lid 2 is closed, the elastic light shield fits tightly against the opening of the sample tube 9, forming a sealed optical path environment to eliminate interference from external ambient light.

[0053] The front end of the spectral sensor 802 is provided with a narrowband filter with center wavelengths of 540nm and 576nm, which correspond to two characteristic absorption peaks of oxyhemoglobin, respectively, and are used to selectively receive the intensity of reflected light from these two wavelengths.

[0054] Example 3: The process and implementation of the monitoring method. Please refer to the following document. Figure 5 Based on the aforementioned transport box, this embodiment provides a method for real-time monitoring of sample status, including the following steps:

[0055] S1: Temperature compensation control. The temperature sensor 7 collects the internal temperature of the chamber in real time. The microcontroller 6 compares the collected temperature with the preset range of 2℃-8℃. When the temperature is higher than 8℃ (first preset threshold), the microcontroller 6 supplies power to the semiconductor cooling chip 5 to cool its cold end, and at the same time starts the micro fan 13 for forced heat dissipation, transferring the cold energy to the heat-insulating inner liner 3. When the temperature is lower than 2℃ (second preset threshold), the microcontroller 6 controls the semiconductor cooling chip 5 to reverse the power supply, turning its cold end into a hot end, thus achieving heat compensation. This process uses a PID control algorithm to ensure that the temperature quickly returns to and stabilizes within the set range.

[0056] S2: Hemolysis status detection. During transport, the system can start hemolysis detection at a preset time point (e.g., every 2 hours) or when triggered by the user via a remote terminal. The microcontroller 6 controls the light source 801 to emit detection light to the sample tube 9, and the spectral sensor 802 receives the reflectance spectrum. The microcontroller 6 extracts the ratio of absorbance at dual wavelengths of 540nm and 576nm of the reflectance spectrum and compares it with the built-in standard hemolysis sample curve to calculate the hemolysis index. When the hemolysis index exceeds the preset threshold (e.g., 15%), the sample is determined to be hemolyzed, and a hemolysis alarm message is generated.

[0057] The standard hemolysis sample curve is pre-established and stored in the microcontroller 6 using the following method:

[0058] (1) Preparation of standard hemolyzed samples: Fresh anticoagulated whole blood was taken, and plasma was separated by centrifugation. The hemoglobin standard was diluted to a gradient concentration using phosphate buffer (PBS) at 0 g / L, 0.5 g / L, 1.0 g / L, 2.0 g / L, 4.0 g / L, 8.0 g / L and 16.0 g / L. The hemoglobin solutions of each gradient concentration were mixed with homologous red blood cell precipitates at a volume ratio of 1:1 to simulate blood samples with different degrees of hemolysis.

[0059] (2) Spectral acquisition: The above standard hemolysis samples were placed into test tubes of the same specifications as the sample tube (9). Under the same environmental conditions (temperature 4℃, relative humidity 50%), the intensity of reflected light at 540nm and 576nm of each sample was acquired by the light reflection hemolysis detection module (8), and the absorbance ratio R = A540 / A576 was calculated.

[0060] (3) Curve fitting: Using the degree of hemolysis (i.e., hemoglobin concentration, g / L) as the abscissa and the absorbance ratio R as the ordinate, the least squares method is used to fit the curve of the standard hemolyzed sample. The fitting equation is R = a·C + b, where C is the hemoglobin concentration and a and b are fitting constants. The microcontroller 6 substitutes the absorbance ratio of the measured sample into the curve equation to calculate the corresponding hemoglobin concentration, which is then output as the hemolysis index.

[0061] S3: Leakage detection. During the entire transfer process, the microcontroller 6 continuously monitors the resistance value between the two flexible conductive films 101 in the leak-proof sensing layer 10. When the resistance value is lower than the preset resistance threshold (such as 100kΩ), it is determined that liquid leakage has occurred at the bottom of the heat-insulating inner liner 3, and a leakage alarm signal is generated. This alarm signal can simultaneously trigger the LED indicator light outside the box 1 to flash and issue an alarm through the buzzer.

[0062] S4: Data upload and remote monitoring. The microcontroller 6 transmits the collected real-time temperature data, hemolysis alarm information and leakage alarm information to the remote monitoring platform (such as the Laboratory Information Management System LIS) in real time through the wireless communication module 11. The management personnel can view the real-time status of the transport box, historical temperature curves and alarm records through the monitoring platform to achieve transparent management of the entire sample transport process.

[0063] Example 4: A preferred embodiment, such as Figure 4 and Figure 5 As shown, in order to further improve the stability of sample placement and battery life, a support frame and a battery placement rack can also be provided inside the housing 1 of the present invention.

[0064] The support frame is used to fix the sample tube 9 so that its opening faces the light reflection hemolysis detection module 8, ensuring that the detection light path is aligned. The battery holder is equipped with a rechargeable lithium battery to provide power to the semiconductor cooling chip 5, microcontroller 6, micro fan 13 and wireless communication module 11. In long-distance transportation scenarios, it can be continuously powered by an external vehicle power supply or portable power supply to ensure the continuity of active temperature control.

[0065] Once the transport box arrives at its destination, the recipient can scan the QR code on box 1 to obtain complete temperature curves, hemolysis detection records, and leakage records from the remote monitoring platform. Only after confirming that the sample is qualified can the recipient sign for it and proceed with the testing process.

[0066] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Those skilled in the art can readily implement the present invention based on the accompanying drawings and the above description. However, any modifications, alterations, or variations made by those skilled in the art without departing from the scope of the present invention, utilizing the disclosed technical content, are equivalent embodiments of the present invention. Furthermore, any modifications, alterations, or variations made to the above embodiments based on the essential technology of the present invention are still within the protection scope of the present invention.

Claims

1. A blood sample refrigerated transport box with temperature compensation function, comprising a box body (1), a box lid (2), a phase change cold storage layer (4), a semiconductor refrigeration chip (5), a microcontroller (6), a temperature sensor (7), a sample status monitoring unit, and a sample tube (9), characterized in that: The box (1) is equipped with an insulated inner liner (3); The phase change cold storage layer (4) is disposed between the outer wall of the heat-insulating inner liner (3) and the inner wall of the box body (1); The cold end of the semiconductor cooling chip (5) is attached to the outer wall of the heat-insulating inner liner (3), and its hot end is connected to a heat dissipation device. The microcontroller (6) is disposed in the interlayer of the housing (1); The temperature sensor (7) is installed on the inner wall of the heat-insulating inner liner (3) and is electrically connected to the microcontroller (6); The sample status monitoring unit includes a light-reflective hemolysis detection module (8) and a leak-proof sensing layer (10). The light-reflective hemolysis detection module (8) is located inside the box cover (2) and is used to emit detection light to the sample tube (9) and receive reflected light. The leak-proof sensing layer (10) is laid at the bottom of the heat-insulating inner liner (3) and is composed of a conductive mesh arranged at intervals; The microcontroller (6) is electrically connected to the semiconductor cooling chip (5), the temperature sensor (7), the light reflection hemolysis detection module (8), and the leak-proof sensing layer (10), and is configured to: when the temperature detected by the temperature sensor (7) is higher than the first preset threshold or lower than the second preset threshold, the semiconductor cooling chip (5) is activated to perform cooling or heating compensation.

2. The blood sample refrigerated transport box with temperature compensation function according to claim 1, characterized in that, The light reflection hemolysis detection module (8) includes a light source (801) and a spectral sensor (802). The light source (801) emits visible light with a wavelength range of 500nm-700nm. The spectral sensor (802) is used to receive the reflection spectrum of the blood sample in the sample tube (9). The microcontroller (6) determines the degree of hemolysis based on the intensity change of the characteristic absorption peak of hemoglobin in the reflection spectrum.

3. The blood sample refrigerated transport box with temperature compensation function according to claim 1, characterized in that, The leak-proof sensing layer (10) includes two parallel flexible conductive films (101) and an insulating mesh (102) between the two flexible conductive films. When liquid seeps in, the two flexible conductive films (101) become conductive, and the microcontroller (6) detects the decrease in resistance and generates a leak alarm signal.

4. The blood sample refrigerated transport box with temperature compensation function according to claim 1, characterized in that, It also includes a wireless communication module (11), which is electrically connected to the microcontroller (6) and is used to upload temperature data, hemolysis status data and leakage alarm data to the remote monitoring platform in real time.

5. A blood sample refrigerated transport box with temperature compensation function according to claim 1, characterized in that, The heat dissipation device includes heat dissipation fins (12) and a micro fan (13). The heat dissipation fins (12) are attached to the hot end of the semiconductor cooling chip (5), and the micro fan (13) is positioned toward the heat dissipation fins (12).

6. A method for real-time monitoring of sample status based on the transport box according to any one of claims 1 to 5, characterized in that, Includes the following steps: S1, the temperature inside the box is collected in real time by temperature sensor (7). When the temperature deviates from the preset range of 2℃-8℃, the microcontroller (6) controls the semiconductor cooling chip (5) to start and perform cooling or heating temperature compensation. S2, at a preset time point during the transport process or when triggered by the user, the light reflection hemolysis detection module (8) emits detection light to the sample tube (9), collects the reflection spectrum, and the microcontroller (6) calculates the hemolysis index. If the hemolysis index exceeds the preset threshold, a hemolysis alarm is generated. S3, the leak-proof sensing layer (10) monitors the resistance value between the conductive meshes in real time. When the resistance value is lower than the preset resistance threshold, it is determined that a liquid leak has occurred and a leak alarm is generated. S4, the microcontroller (6) sends temperature data, hemolysis alarm information and leakage alarm information to the remote monitoring terminal through the wireless communication module (11).

7. The real-time sample status monitoring method according to claim 6, characterized in that, In step S2, the hemolysis index is calculated by extracting the ratio of absorbance at dual wavelengths of 540nm and 576nm from the reflectance spectrum and comparing it with the curve of a standard hemolysis sample.

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