Children skin soothing and repairing cream containing egg yolk oil extract and preparation method of children skin soothing and repairing cream

Abstract

The invention relates to the technical field of child skin care products, in particular to a child skin soothing and repairing cream containing egg yolk oil extract and a preparation method of the child skin soothing and repairing cream. The prepared children skin soothing and repairing cream is long in acting time and high in repairing efficiency; wherein the egg yolk oil hydrogel beads are easy to break at the damaged skin, and the egg yolk oil emulsion is released, so that the action time can be prolonged, the egg yolk oil emulsion is released at the damaged skin in a targeted manner, and the utilization rate and the repair efficiency are effectively improved; the silk fibroin microspheres reduce active oxygen and have good ultraviolet resistance; through the synergistic effect of the components in the plant extract, various problems of fragile skin barrier, dryness, water shortage, redness, inflammation and the like of children can be effectively solved; the auxiliary materials can promote mutual fusion of the components while playing a role in moistening and moisturizing, and compared with the prior art, the composition has a wide application prospect.

Description

Technical Field

[0001] This invention relates to the field of children's skin care products technology, and in particular to a children's skin soothing and repairing cream containing egg yolk oil extract and its preparation method. Background Technology

[0002] The skin is the largest organ in the human body, playing a vital role in protection, excretion, temperature regulation, and sensing external stimuli. Skin is frequently exposed to factors such as oxidation, dryness, and ultraviolet radiation, making it prone to damage. Without proper care, skin condition will deteriorate, leading to dryness, dehydration, and roughness.

[0003] Children's skin is delicate, with a thinner stratum corneum, underdeveloped sebaceous and sweat glands, and a weaker skin barrier function. This makes them susceptible to external environmental factors such as dryness, friction, ultraviolet radiation, and microorganisms, leading to problems like dryness, itching, redness, and inflammation. Therefore, children's skincare products need to be gentle, non-irritating, and have soothing and repairing properties.

[0004] Egg yolk oil extract is an oil extracted from egg yolks. It is rich in nutrients such as unsaturated fatty acids, lecithin, and vitamins A, D, and E. These components have moisturizing, skin barrier repair, anti-inflammatory, and antioxidant effects, making it beneficial for the delicate skin of children.

[0005] However, there are relatively few children's skin care products containing egg yolk oil on the market. Usually, a children's skin soothing and repair cream is made by simply mixing various effective ingredients with different effects, such as egg yolk oil extract. While this method can have some effect on anti-inflammatory and moisturizing effects, its effect is short-lived and requires frequent application. The skin repair efficiency needs to be further improved.

[0006] Therefore, based on the relevant technologies mentioned above, there is an urgent need to develop a children's skin soothing and repair cream containing egg yolk oil extract and its preparation method. Summary of the Invention

[0007] In view of this, the purpose of this invention is to provide a children's skin soothing and repair cream containing egg yolk oil extract and its preparation method, so as to solve the problems of short action time and poor repair efficiency of existing children's skin soothing and repair creams.

[0008] To achieve the above objectives, the present invention provides a children's skin soothing and repairing cream containing egg yolk oil extract and its preparation method.

[0009] A children's skin soothing and repairing cream containing egg yolk oil extract, comprising the following ingredients by weight: Egg yolk oil hydrogel beads 3-6 parts; silk fibroin microspheres 1.2-4 parts; plant extracts 0.5-3 parts; auxiliary materials 87-102 parts; The egg yolk oil hydrogel beads were prepared from egg yolk oil emulsion, soybean protein nanofibers and sodium alginate. The plant extract is obtained by mixing purslane extract, saffron extract, quinoa seed extract, artemisia capillaris extract and chlorella extract; The auxiliary materials include phase A, phase B, and phase C; Phase A is prepared from propylene glycol, nicotinamide, isopentyl glycol and deionized water; Phase B was prepared from egg yolk oil extract, shea butter oil, and glyceryl stearate. The C phase is chlorhexidine digluconate.

[0010] Preferably, the silk fibroin microspheres are prepared as follows: Step A1. Cut the silkworm cocoons into small pieces and boil them in a sodium carbonate aqueous solution for 30-50 minutes. Collect the degummed silk fibers, dry them, and then add them to a lithium bromide aqueous solution. Stir until completely dissolved, dialyze with deionized water for 3-4 days, and centrifuge to obtain a silk fibroin aqueous solution. Step A2. Add ethanol dropwise to the silk fibroin aqueous solution, sonicate for 2-4 min, freeze at -24 to -18°C for 22-24 h, thaw at room temperature, centrifuge, collect the precipitate, wash with deionized water 3-4 times, freeze dry to obtain silk fibroin microspheres.

[0011] Preferably, the ratio of silkworm cocoons, sodium carbonate aqueous solution, and lithium bromide aqueous solution used in step A1 is 4-5g:400-500mL:200-270mL; The mass concentration of the sodium carbonate aqueous solution is 5-8 g / L; The mass concentration of the lithium bromide aqueous solution is 800-807 g / L; The mass fraction of the silk fibroin aqueous solution is 2.5%-4%.

[0012] Preferably, the volume ratio of ethanol and silk fibroin aqueous solution in step A2 is 2.5-4:15-24.

[0013] Preferably, the egg yolk oil hydrogel beads are prepared as follows: Step B1. Mix deionized water, compound emulsifier and egg yolk oil extract, and sonicate for 2-4 minutes to obtain egg yolk oil emulsion; Step B2. Disperse 0.5-1g of soy protein isolate in 100-200mL of deionized water, adjust the pH to 2 with 6-7mol / L hydrochloric acid, and stir magnetically for 2-3h at room temperature. Then, place it at 2-4°C overnight until completely hydrated. Centrifuge, take the supernatant, and adjust the pH to 2 with 6-7mol / L hydrochloric acid to obtain solution A. Step B3. Heat solution A in a water bath. After the reaction is complete, cool it in an ice-water bath at 0-4°C. Then adjust the pH to 7-8 with sodium hydroxide at a mass concentration of 6-7 mol / L. Freeze-dry to obtain soybean protein nanofibers. Step B4. Dissolve soybean protein nanofibers and sodium alginate separately in deionized water to obtain soybean protein nanofiber aqueous solution and sodium alginate aqueous solution. Then mix the soybean protein nanofiber aqueous solution, sodium alginate aqueous solution and egg yolk oil emulsion, and stir thoroughly to obtain mixed solution B. Use a peristaltic pump to drop mixed solution B into calcium chloride with a mass concentration of 0.1 mol / L at a rate of 1-2 rpm. After the addition is complete, soak for 30-50 minutes, wash with deionized water 3-4 times, and freeze dry to obtain egg yolk oil hydrogel beads.

[0014] Preferably, the mass ratio of deionized water, composite emulsifier and egg yolk oil extract in step B1 is 90-105:1.9-3.2:5.5-8; The composite emulsifier is obtained by mixing soy protein isolate and Tween 80 at a mass ratio of 2-3:1.

[0015] Preferably, the water bath heating in step B3 is performed at a temperature of 80-88°C for 10-12 hours.

[0016] Preferably, the mass fraction of the soybean protein nanofiber aqueous solution in step B4 is 1%-2%; The sodium alginate aqueous solution has a mass fraction of 1%-2%; The volume ratio of the soybean protein nanofiber aqueous solution, sodium alginate aqueous solution, and egg yolk oil emulsion is 70-80:20-30:1-2.

[0017] Preferably, the plant extract is obtained by mixing purslane extract, saffron extract, quinoa seed extract, artemisia capillaris extract and chlorella extract in a mass ratio of 5-8:2.2-4.7:2.2-4.7:1-2:1-2; Phase A comprises the following raw materials in parts by weight: Propylene glycol 12-16 parts; Nicotinamide 1-2 parts; Isopentyl glycol 0.5-1 part; Allantoin 0.1-0.2 parts; Sodium gluconate 0.01-0.05 parts; Capryloyl hydroxamic acid 0.05-0.1 parts; Polyquaternium salt 1-2 parts; p-hydroxyacetophenone 0.1-0.3 parts; Carbomer 0.1-0.3 parts; Deionized water 74-79 parts; Phase B comprises the following raw materials in parts by weight: Egg yolk oil extract 2-4 parts; patchouli leaf extract 0.5-0.8 parts; chamomile flower extract 0.3-0.5 parts; licorice root extract 0.0001-0.001 parts; centella asiatica root extract 0.0001-0.001 parts; peony seed oil 0.5-0.8 parts; sunflower seed oil 0.5-0.8 parts; squalane 2-4 parts; bisabolol 0.3-0.6 parts; shea butter oil 3-6 parts; glyceryl stearate 1-3 parts; cetearyl alcohol 1-3 parts; dimethicone 1-3 parts; tocopherol 1-3 parts; The C phase comprises the following raw materials in parts by weight: Chlorhexidine digluconate 1-2 parts.

[0018] A method for preparing a children's skin soothing and repairing cream containing egg yolk oil extract includes the following steps: Step S1. Add deionized water to a water pot, add phase A raw material while stirring, heat and maintain the temperature at 82-90°C, stir for 30-50 minutes, and keep warm to obtain mixture 1; Step S2. Add phase B raw materials to the oil pot in sequence, and heat to 75-80°C while stirring. After complete dissolution, keep warm for later use to obtain mixture 2. Step S3. Mixture 1 is vacuum-inhaled into an emulsification pot, then mixture 2 is added, and the mixture is continuously stirred at 82-88°C for 5-8 minutes. After vacuum degassing for 10-15 minutes, the mixture is cooled to 35-40°C and then the C phase raw material is added to obtain the auxiliary material. Step S4. Add egg yolk oil hydrogel beads, silk fibroin microspheres and plant extracts to the excipients at 35-40°C, stir well, and you will get the children's skin soothing and repairing cream.

[0019] The beneficial effects of this invention are: This invention provides a children's skin soothing and repair cream containing egg yolk oil extract and its preparation method. The invention prepares a children's skin soothing and repair cream with long-lasting action and high repair efficiency by mixing egg yolk oil hydrogel beads, silk fibroin microspheres, plant extracts, and auxiliary materials. Specifically, egg yolk oil hydrogel beads are formed by cross-linking soybean protein nanofibers and sodium alginate in calcium chloride solution. When the skin is damaged, the damaged area is weakly alkaline. The carboxyl groups in sodium alginate easily dissociate in a weakly alkaline environment, and the calcium ions in the cross-linked network easily combine with free hydroxyl groups, causing the hydrogel beads to rupture and releasing the egg yolk oil emulsion. These egg yolk oil hydrogel beads can prolong the action time, target the release to the damaged skin area, effectively improve utilization and repair efficiency, reduce oxidative loss, and are beneficial for skin barrier repair. Silk fibroin molecules contain a large number of hydrophilic groups, providing long-lasting and gentle moisturizing effects. Silk fibroin microspheres generated by ethanol-induced self-assembly of silk fibroin retain the functional groups of aromatic amino acid residues and are rich in serine and tyrosine, which can provide active hydroxyl and phenolic hydroxyl groups to react with free radicals, thus helping to reduce reactive oxygen species and providing good UV resistance. Through the synergistic effect between the components in plant extracts, various problems such as fragile skin barrier, dryness, redness, and inflammation in children can be effectively solved. The auxiliary materials not only play a moisturizing role, but also promote the fusion between components and improve the overall efficacy. Compared with existing technologies, it has broad application prospects. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to specific embodiments.

[0021] The sources and properties of some of the raw materials used in this invention are as follows: Peony seed oil was purchased from Heze Yaoshun Peony Biotechnology Co., Ltd.; Artemisia capillaris extract was purchased from Shanghai Jiakai Biotechnology Co., Ltd.; and Kunuo Ali seed extract was purchased from Shanghai Jiakai Biotechnology Co., Ltd.

[0022] Example 1: A method for preparing a children's skin soothing and repairing cream containing egg yolk oil extract, comprising the following steps: S1. Cut 4g of silkworm cocoons into small pieces and boil them in 400mL of sodium carbonate aqueous solution with a mass concentration of 5g / L for 30min. Collect the degummed silk fibers, dry them, and then add them to 200mL of lithium bromide aqueous solution with a mass concentration of 800g / L. Stir until completely dissolved, dialyze with deionized water for 3 days, and centrifuge to obtain a silk fibroin aqueous solution with a mass fraction of 2.5%. S2. Add 2.5 mL of ethanol to 15 mL of silk fibroin aqueous solution, sonicate for 2 min, freeze at -24°C for 22 h, thaw at room temperature, centrifuge, collect the precipitate, wash three times with deionized water, freeze dry to obtain silk fibroin microspheres. S3. Mix 90g of deionized water, 1.9g of compound emulsifier and 5.5g of egg yolk oil extract. The compound emulsifier is obtained by mixing soy protein isolate and Tween 80 at a mass ratio of 2:1. After ultrasonic treatment for 2 minutes, egg yolk oil emulsion is obtained. S4. Disperse 0.5g of soy protein isolate in 100mL of deionized water, adjust the pH to 2 with 6mol / L hydrochloric acid, stir magnetically for 2h at room temperature, and then place at 2°C overnight until completely hydrated. Centrifuge, take the supernatant and adjust the pH to 2 with 6mol / L hydrochloric acid to obtain solution A; S5. Heat solution A in a water bath at 80°C for 10 hours. After the reaction is complete, cool it in an ice-water bath at 0°C. Then adjust the pH to 7 with sodium hydroxide at a mass concentration of 6 mol / L and freeze-dry to obtain soybean protein nanofibers. S6. Soybean protein nanofibers and sodium alginate were dissolved in deionized water to obtain a 1% (w / w) aqueous solution of soybean protein nanofibers and a 1% (w / w) aqueous solution of sodium alginate. 70 mL of the soybean protein nanofiber aqueous solution, 20 mL of the sodium alginate aqueous solution, and 1 mL of egg yolk oil emulsion were mixed and stirred thoroughly to obtain mixed solution B. Mixed solution B was added dropwise to 0.1 mol / L calcium chloride at a rate of 1 rpm using a peristaltic pump. After the addition was completed, the mixture was soaked for 30 min, washed three times with deionized water, and freeze-dried to obtain egg yolk oil hydrogel beads. S7. Mix 5g of purslane extract, 2.2g of saffron extract, 2.2g of quinoa seed extract, 1g of artemisia capillaris extract and 1g of chlorella extract to obtain plant extract; add deionized water to a water pot, add phase A raw material while stirring, heat and maintain the temperature at 82°C, stir for 30 minutes, keep warm for later use to obtain mixture 1; S8. Add phase B raw materials to the oil pot in sequence, heat to 75°C while stirring, and keep warm after complete dissolution to obtain mixture 2; S9. Mixture 1 is vacuum-inhaled into an emulsification pot, then mixture 2 is added, and the mixture is continuously stirred at 82°C for 5 minutes. After vacuum degassing for 10 minutes, the mixture is cooled to 35°C and then the C phase raw material is added to obtain the auxiliary material. S10. Add 3g of egg yolk oil hydrogel beads, 1.2g of silk fibroin microspheres and 0.5g of plant extract to 87g of excipient materials at 35°C, stir well, and you will get a children's skin soothing and repairing cream.

[0023] Example 2: A method for preparing a children's skin soothing and repairing cream containing egg yolk oil extract, comprising the following steps: S1. Cut 4.35g of silkworm cocoons into small pieces and boil them in 435mL of sodium carbonate aqueous solution with a mass concentration of 6g / L for 35min. Collect the degummed silk fibers, dry them, and then add them to 220mL of lithium bromide aqueous solution with a mass concentration of 803g / L. Stir until completely dissolved, dialyze with deionized water for 3 days, and centrifuge to obtain a 3% silk fibroin aqueous solution. S2. Add 3 mL of ethanol to 18 mL of silk fibroin aqueous solution, sonicate for 3 min, freeze at -22°C for 23 h, thaw at room temperature, centrifuge, collect the precipitate, wash 3 times with deionized water, freeze dry to obtain silk fibroin microspheres. S3. Mix 95g of deionized water, 2.4g of compound emulsifier and 6.3g of egg yolk oil extract. The compound emulsifier is obtained by mixing soy protein isolate and Tween 80 at a mass ratio of 2:1. After ultrasonic treatment for 3 minutes, egg yolk oil emulsion is obtained. S4. Disperse 0.7g of soy protein isolate in 140mL of deionized water, adjust the pH to 2 with 6mol / L hydrochloric acid, and stir magnetically for 2h at room temperature. Then, place it at 3°C ​​overnight until it is completely hydrated. Centrifuge, take the supernatant and adjust the pH to 2 with 6mol / L hydrochloric acid to obtain solution A. S5. Solution A was heated in a water bath at 82°C for 11 hours. After the reaction was completed, it was cooled in an ice-water bath at 2°C. The pH was then adjusted to 7 with sodium hydroxide at a mass concentration of 6 mol / L. The solution was then freeze-dried to obtain soybean protein nanofibers. S6. Soybean protein nanofibers and sodium alginate were dissolved separately in deionized water to obtain a 1% (w / w) aqueous solution of soybean protein nanofibers and a 1% (w / w) aqueous solution of sodium alginate. Then, 73 mL of the soybean protein nanofiber aqueous solution, 23 mL of the sodium alginate aqueous solution and 1.3 mL of egg yolk oil emulsion were mixed and stirred thoroughly to obtain mixed solution B. Mixed solution B was added dropwise to calcium chloride with a mass concentration of 0.1 mol / L at a rate of 1.3 rpm using a peristaltic pump. After the addition was completed, the mixture was soaked for 35 min, washed 3 times with deionized water, and freeze-dried to obtain egg yolk oil hydrogel beads. S7. Mix 6g of purslane extract, 3g of saffron extract, 3g of quinoa seed extract, 1.3g of artemisia capillaris extract and 1.3g of chlorella extract to obtain plant extract; add deionized water to a water pot, add phase A raw material while stirring, heat and maintain the temperature at 85°C, stir for 35 minutes, keep warm for later use to obtain mixture 1; S8. Add the B phase raw material to the oil pot in sequence, and heat to 77°C while stirring. After it is completely dissolved, keep it warm for later use to obtain mixture 2. S9. Mixture 1 is vacuum-inhaled into an emulsifying pot, then mixture 2 is added, and the mixture is continuously stirred at 84°C for 6 minutes. After vacuum degassing for 12 minutes, the mixture is cooled to 37°C and then the C-phase raw material is added to obtain the auxiliary material. S10. Add 4g of egg yolk oil hydrogel beads, 2.2g of silk fibroin microspheres and 1.4g of plant extract to 92g of excipient materials at 37°C, stir well to obtain children's skin soothing and repairing cream.

[0024] Example 3: A method for preparing a children's skin soothing and repairing cream containing egg yolk oil extract, comprising the following steps: S1. Cut 4.7g of silkworm cocoons into small pieces and boil them in 470mL of sodium carbonate aqueous solution with a mass concentration of 7g / L for 40min. Collect the degummed silk fibers, dry them, and then add them to 250mL of lithium bromide aqueous solution with a mass concentration of 805g / L. Stir until completely dissolved, dialyze with deionized water for 4 days, and centrifuge to obtain a 34% silk fibroin aqueous solution. S2. Add 3.5 mL of ethanol to 21 mL of silk fibroin aqueous solution, sonicate for 3 min, freeze at -20°C for 23 h, thaw at room temperature, centrifuge, collect the precipitate, wash with deionized water 4 times, freeze dry to obtain silk fibroin microspheres. S3. Mix 100g deionized water, 2.8g compound emulsifier and 7.1g egg yolk oil extract. The compound emulsifier is obtained by mixing soy protein isolate and Tween 80 at a mass ratio of 3:1. After ultrasonic treatment for 3 minutes, egg yolk oil emulsion is obtained. S4. Disperse 0.9g of soy protein isolate in 170mL of deionized water, adjust the pH to 2 with 7mol / L hydrochloric acid, and stir magnetically for 3h at room temperature. Then place it at 4°C overnight until completely hydrated. Centrifuge, take the supernatant and adjust the pH to 2 with 7mol / L hydrochloric acid to obtain solution A. S5. Solution A was heated in a water bath at 85°C for 11 hours. After the reaction was completed, it was cooled in an ice-water bath at 3°C. The pH was then adjusted to 8 with sodium hydroxide at a mass concentration of 7 mol / L. The solution was then freeze-dried to obtain soybean protein nanofibers. S6. Soybean protein nanofibers and sodium alginate were dissolved separately in deionized water to obtain a 2% (w / w) aqueous solution of soybean protein nanofibers and a 2% (w / w) aqueous solution of sodium alginate. Then, 77 mL of the soybean protein nanofiber aqueous solution, 27 mL of the sodium alginate aqueous solution and 1.7 mL of egg yolk oil emulsion were mixed and stirred thoroughly to obtain mixed solution B. Mixed solution B was added dropwise to calcium chloride with a mass concentration of 0.1 mol / L at a rate of 1.7 rpm using a peristaltic pump. After the addition was completed, the mixture was soaked for 40 min, washed 4 times with deionized water, and freeze-dried to obtain egg yolk oil hydrogel beads. S7. Mix 7g of purslane extract, 3.8g of saffron extract, 3.8g of quinoa seed extract, 1.7g of artemisia capillaris extract and 1.7g of chlorella extract to obtain plant extracts; add deionized water to a water pot, add phase A raw material while stirring, heat and maintain the temperature at 87°C, stir for 450 minutes, and keep warm for later use to obtain mixture 1; S8. Add phase B raw materials to the oil pot in sequence, heat to 79°C while stirring, and keep warm after complete dissolution to obtain mixture 2; S9. Mixture 1 is vacuum-inhaled into an emulsification pot, then mixture 2 is added, and the mixture is continuously stirred at 86°C for 7 minutes. After vacuum degassing for 14 minutes, the mixture is cooled to 39°C and then the C phase raw material is added to obtain the auxiliary material. S10. Add 5g of egg yolk oil hydrogel beads, 3.1g of silk fibroin microspheres and 2.2g of plant extract to 97g of excipient materials at 39°C, stir well to obtain children's skin soothing and repairing cream.

[0025] Example 4: A method for preparing a children's skin soothing and repairing cream containing egg yolk oil extract, comprising the following steps: S1. Cut 5g of silkworm cocoons into small pieces and boil them in 500mL of sodium carbonate aqueous solution with a mass concentration of 8g / L for 50min. Collect the degummed silk fibers, dry them, and then add them to 270mL of lithium bromide aqueous solution with a mass concentration of 807g / L. Stir until completely dissolved, dialyze with deionized water for 4 days, and centrifuge to obtain a 4% silk fibroin aqueous solution. S2. Add 4 mL of ethanol to 24 mL of silk fibroin aqueous solution, sonicate for 4 min, freeze at -18°C for 24 h, thaw at room temperature, centrifuge, collect the precipitate, wash 4 times with deionized water, freeze dry to obtain silk fibroin microspheres. S3. Mix 105g of deionized water, 3.2g of compound emulsifier and 8g of egg yolk oil extract. The compound emulsifier is obtained by mixing soy protein isolate and Tween 80 at a mass ratio of 3:1. After ultrasonic treatment for 4 minutes, egg yolk oil emulsion is obtained. S4. Disperse 1g of soy protein isolate in 200mL of deionized water, adjust the pH to 2 with 7mol / L hydrochloric acid, stir magnetically for 3h at room temperature, and then place at 4°C overnight until completely hydrated. Centrifuge, take the supernatant and adjust the pH to 2 with 7mol / L hydrochloric acid to obtain solution A. S5. Solution A was heated in a water bath at 88°C for 12 hours. After the reaction was completed, it was cooled in an ice-water bath at 4°C. The pH was then adjusted to 8 with sodium hydroxide at a mass concentration of 7 mol / L. The solution was then freeze-dried to obtain soybean protein nanofibers. S6. Soybean protein nanofibers and sodium alginate were dissolved in deionized water to obtain a 2% (w / w) aqueous solution of soybean protein nanofibers and a 2% (w / w) aqueous solution of sodium alginate. 80 mL of the soybean protein nanofiber aqueous solution, 30 mL of the sodium alginate aqueous solution, and 2 mL of egg yolk oil emulsion were mixed and stirred thoroughly to obtain mixed solution B. Mixed solution B was then added dropwise to 0.1 mol / L calcium chloride at a rate of 2 rpm using a peristaltic pump. After the addition was completed, the mixture was soaked for 50 min, washed 4 times with deionized water, and freeze-dried to obtain egg yolk oil hydrogel beads. S7. Mix 8g of purslane extract, 4.7g of saffron extract, 4.7g of quinoa seed extract, 2g of artemisia capillaris extract and 2g of chlorella extract to obtain plant extract; add deionized water to a water pot, add phase A raw material while stirring, heat and maintain the temperature at 90°C, stir for 50 minutes, keep warm for later use to obtain mixture 1; S8. Add phase B raw materials to the oil pot in sequence, heat to 80°C while stirring, and keep warm after complete dissolution to obtain mixture 2; S9. Mixture 1 is vacuum-inhaled into an emulsifying pot, then mixture 2 is added, and the mixture is continuously stirred at 88°C for 8 minutes. After vacuum degassing for 15 minutes, the mixture is cooled to 40°C and then the C-phase raw material is added to obtain the auxiliary material. S10. Add 6g of egg yolk oil hydrogel beads, 4g of silk fibroin microspheres and 3g of plant extract to 102g of excipients at 40°C, stir well to obtain children's skin soothing and repairing cream.

[0026] Comparative Example 1: Compared with Example 1, this comparative example did not add egg yolk oil hydrogel beads in the preparation process of the children's skin soothing and repair cream. All other steps and parameters were the same, and will not be repeated here. The final product was the children's skin soothing and repair cream.

[0027] Comparative Example 2: Compared with Example 1, this comparative example did not add silk fibroin microspheres in the preparation process of the children's skin soothing and repair cream. All other steps and parameters were the same, and will not be repeated here. The final product was the children's skin soothing and repair cream.

[0028] Comparative Example 3: Compared with Example 1, this comparative example did not add plant extracts during the preparation of the children's skin soothing and repair cream. All other steps and parameters were the same, and will not be repeated here. The final product was the children's skin soothing and repair cream.

[0029] Comparative Example 4: This comparative example differs from Example 1 only in that "silk fibroin microspheres" are replaced with "silk fibroin". All other steps and parameters are the same, and will not be repeated here. The final product is a children's skin soothing and repair cream.

[0030] Comparative Example 5: This comparative example differs from Example 1 only in that the "egg yolk oil hydrogel beads" are replaced with "egg yolk oil extract". All other steps and parameters are the same, and will not be repeated here. The final product is a children's skin soothing and repair cream.

[0031] Comparative Example 6: Compared with Example 1, this comparative example only replaces "5g purslane extract, 2.2g saffron extract, 2.2g quinoa seed extract, 1g artemisia capillaris extract and 1g chlorella extract" with "5g purslane extract, 5g saffron extract, 5g quinoa seed extract, 5g artemisia capillaris extract and 5g chlorella extract". All other steps and parameters are the same, and will not be repeated in this comparative example. The final product is a children's skin soothing and repairing cream.

[0032] Comparative Example 7: Compared with Example 1, this comparative example only replaces "2.5% silk fibroin aqueous solution" with "10% silk fibroin aqueous solution". All other steps and parameters are the same, and will not be repeated here. The final product is a children's skin soothing and repair cream.

[0033] Comparative Example 8: Compared with Example 1, this comparative example only replaces "2.5% silk fibroin aqueous solution" with "1% silk fibroin aqueous solution". All other steps and parameters are the same, and will not be repeated here. The final product is a children's skin soothing and repair cream.

[0034] Performance testing: Soothing and restorative: A total of 160 healthy male or female subjects aged 18-40 years were enrolled, and 144 subjects completed the entire test. Inclusion criteria: (1) Healthy Asian individuals aged 18-40 years, regardless of gender, with female subjects accounting for at least 80%; (2) Individuals with highly sensitive skin according to the Bauman questionnaire and lactate test (Bauman questionnaire score 50-72 points, and lactate test score ≥6); (3) Facial skin type determined by a dermatologist to be normal, dry, or combination-dry; (4) Individuals in good health, without any other chronic diseases or diseases currently being treated; (5) Individuals who have not used hormone drugs or immunosuppressants in the past month; (6) Individuals who have not participated in other clinical trials on the test site in the past three months. (7) Has not undergone cosmetic procedures such as laser chemical peels; (8) Has stopped using skin care products similar to the test product during the trial period; (9) Will not participate in any other clinical trials at other research centers during the trial period; (10) Agrees not to use any cosmetics, drugs, or health products that may affect the results during the trial period; (11) Subjects should not use skin care products with redness-reducing effects 24 hours before participating in the trial; (12) Has no ethical contraindications; (13) Has read and signed the informed consent form before the study; (14) Is aware of the project and is willing and able to comply with all testing requirements.

[0035] Exclusion criteria: (1) Those with severe systemic diseases, currently using or within 2 weeks prior to the start of this study, using systemic or topical corticosteroids, vasoconstrictors, antibiotics, anti-inflammatory drugs, high-dose vitamin C, beta-carotene, etc.; (2) Those with severe allergic diseases or who have had cosmetic allergies within the past 1-2 years; (3) Those who are pregnant, breastfeeding, or planning to become pregnant; (4) Those who have participated in other clinical trials within the past 3 months prior to the start of this study; (5) Those who work outdoors and cannot avoid sun exposure; (6) Those who have received cosmetic treatments in the test area; (7) Those with a history of skin diseases or other illnesses that may affect the test results, or other conditions that prevent the use of test samples; (8) Other conditions that the researchers deem unsuitable for participation in this trial. Criteria for withdrawal midway: (1) Not selected (e.g., not meeting the selection criteria or being excluded by the exclusion criteria, etc.); (2) The subject is determined to be ineligible after being selected (including but not limited to changes in other lifestyle, diet, skin care habits and skin care products during the evaluation period); (3) The subject chooses to withdraw; (4) An adverse event or a serious adverse event occurs; (5) Loss to follow-up; (6) Force majeure; (7) Other.

[0036] The 144 selected subjects were randomly divided into 12 groups of 12 each. The children's skin soothing and repair creams prepared in Examples 1-4 and Comparative Examples 1-8 were tested. The specific testing method was as follows: Subjects sat quietly for 10-15 minutes in a constant temperature (21±1)℃ and constant humidity (50%±10%) environment. Afterwards, they washed their faces with water. Then, medical filters were placed on both sides of the subject's nasolabial folds. 10% lactic acid (50 μL) and distilled water were randomly dropped onto the filters on both sides. After standing for 1 hour, the subjects cleansed their faces and applied the children's skin soothing and repair creams prepared in Examples 1-4 and Comparative Examples 1-8, twice daily, once in the morning and once in the evening, for 4 consecutive weeks. Subjects then self-rated their scores, calculating a total score and categorizing the total score into four levels: A (>21 points), B (14-21 points), C (8-14 points), and D (≤7 points). Moisturizing properties: One subject was randomly selected from each of the 12 groups. After the skin surface on the inside of the arm was dry and clean, the children's skin soothing and repairing cream prepared in Examples 1-4 and Comparative Examples 1-8 was applied and gently massaged until absorbed. The initial skin humidity of the subject was measured using an electronic skin humidity meter and the average value was recorded as T0. After 2 hours, the skin humidity was measured and recorded as T2. The humidity retention rate was calculated.

[0037] Humidity retention rate = (T2 - T0) / T0 × 100% Table 1 Summary of experimental data from Examples 1-4 and Comparative Examples 1-8

[0038] Table 2 Summary of experimental data from Examples 1-4 and Comparative Examples 1-8

[0039] Table 3 Evaluation Indicators

[0040] Data Analysis: As can be seen from Tables 1, 2, and 3, the children's skin soothing and repairing cream prepared by this invention has better soothing, repairing, and moisturizing properties. This may be due to the formation of hydrogel beads by cross-linking soybean protein nanofibers and sodium alginate in calcium chloride solution. In this process, the hydroxyl and carboxyl groups in sodium alginate combine with the amino acid residues in soybean protein nanofibers through electrostatic interactions and hydrogen bonds. The egg yolk oil emulsion is trapped in the three-dimensional network formed by cross-linking. When the skin is damaged, the damaged area is weakly alkaline. The carboxyl groups in sodium alginate are easily dissociated in a weakly alkaline environment, while the activity of proteases is enhanced in a weakly alkaline environment. The degradation of sodium alginate, as a substrate for enzymes, is accelerated, and the calcium ions in the cross-linked network are also easily combined with free hydroxyl groups. The hydrogel beads then rupture, and the egg yolk oil emulsion is released. These egg yolk oil hydrogel beads can prolong the action time, target the release to the damaged skin area, effectively improve the utilization rate and repair efficiency, reduce oxidative loss, and are beneficial to skin barrier repair. Silk fibroin can also promote the rearrangement and adhesion of damaged stratum corneum. Its molecules contain a large number of hydrophilic groups, providing long-lasting and gentle moisturizing effects. Silk fibroin microspheres generated by ethanol-induced self-assembly of silk fibroin retain the functional groups of aromatic amino acid residues and are rich in serine and tyrosine, which can provide active hydroxyl and phenolic hydroxyl groups to react with free radicals, thus helping to reduce reactive oxygen species and providing good anti-ultraviolet properties. Furthermore, the silk fibroin microspheres can encapsulate their active ingredients inside, enhancing stability. Upon contact with the skin, they slowly disintegrate, gradually releasing the silk fibroin components, effectively prolonging the action time, reducing the need for repeated application, and improving repair efficiency. Purslane extract can inhibit the release of inflammatory factors, promote lipid synthesis in the stratum corneum, and reduce moisture loss; saffron extract inhibits inflammatory pathways, has extremely low irritation, and possesses excellent antioxidant properties; Quinoa seed extract can enhance the integrity of the skin barrier and lock in skin moisture; Artemisia capillaris extract has antibacterial properties and has a good therapeutic effect on common childhood eczema and diaper rash; Artemisia capillaris extract can promote collagen synthesis and accelerate the healing of damaged skin; through the synergistic effect between the components in the plant extracts, it can effectively solve various problems such as fragile skin barrier, dryness, redness, and inflammation in children; the auxiliary materials, while playing a moisturizing role, can also promote the fusion between components and improve the overall efficacy.

[0041] Those skilled in the art should understand that the discussion of any of the above embodiments is merely exemplary and is not intended to imply that the scope of the invention is limited to these examples; within the framework of the invention, the technical features of the above embodiments or different embodiments can also be combined, the steps can be implemented in any order, and there are many other variations of the different aspects of the invention as described above, which are not provided in detail for the sake of brevity.

[0042] This invention is intended to cover all such substitutions, modifications, and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. A children's skin soothing and repairing cream containing egg yolk oil extract, characterized in that, Includes the following quantities of raw materials: Egg yolk oil hydrogel beads 3-6 parts; silk fibroin microspheres 1.2-4 parts; plant extracts 0.5-3 parts; auxiliary materials 87-102 parts; The egg yolk oil hydrogel beads were prepared from egg yolk oil emulsion, soybean protein nanofibers and sodium alginate. The plant extract is obtained by mixing purslane extract, saffron extract, quinoa seed extract, artemisia capillaris extract and chlorella extract; The auxiliary materials include phase A, phase B, and phase C; Phase A is prepared from propylene glycol, nicotinamide, isopentyl glycol and deionized water; Phase B was prepared from egg yolk oil extract, shea butter oil, and glyceryl stearate. The C phase is chlorhexidine digluconate.

2. The children's skin soothing and repairing cream containing egg yolk oil extract according to claim 1, characterized in that, The silk fibroin microspheres are prepared as follows: Step A1. Cut the silkworm cocoons into small pieces and boil them in a sodium carbonate aqueous solution for 30-50 minutes. Collect the degummed silk fibers, dry them, and then add them to a lithium bromide aqueous solution. Stir until completely dissolved, dialyze with deionized water for 3-4 days, and centrifuge to obtain a silk fibroin aqueous solution. Step A2. Add ethanol dropwise to the silk fibroin aqueous solution, sonicate for 2-4 min, freeze at -24 to -18°C for 22-24 h, thaw at room temperature, centrifuge, collect the precipitate, wash with deionized water 3-4 times, freeze dry to obtain silk fibroin microspheres.

3. The children's skin soothing and repairing cream containing egg yolk oil extract according to claim 2, characterized in that, The ratio of silkworm cocoons, sodium carbonate aqueous solution, and lithium bromide aqueous solution used in step A1 is 4-5g:400-500mL:200-270mL; The mass concentration of the sodium carbonate aqueous solution is 5-8 g / L; The mass concentration of the lithium bromide aqueous solution is 800-807 g / L; The mass fraction of the silk fibroin aqueous solution is 2.5%-4%.

4. The children's skin soothing and repairing cream containing egg yolk oil extract according to claim 2, characterized in that, The volume ratio of ethanol and silk fibroin aqueous solution in step A2 is 2.5-4:15-24.

5. The children's skin soothing and repairing cream containing egg yolk oil extract according to claim 1, characterized in that, The method for preparing the egg yolk oil hydrogel beads is as follows: Step B1. Mix deionized water, compound emulsifier and egg yolk oil extract, and sonicate for 2-4 minutes to obtain egg yolk oil emulsion; Step B2. Disperse 0.5-1g of soy protein isolate in 100-200mL of deionized water, adjust the pH to 2 with 6-7mol / L hydrochloric acid, and stir magnetically for 2-3h at room temperature. Then, place it at 2-4°C overnight until completely hydrated. Centrifuge, take the supernatant, and adjust the pH to 2 with 6-7mol / L hydrochloric acid to obtain solution A. Step B3. Heat solution A in a water bath. After the reaction is complete, cool it in an ice-water bath at 0-4°C. Then adjust the pH to 7-8 with sodium hydroxide at a mass concentration of 6-7 mol / L. Freeze-dry to obtain soybean protein nanofibers. Step B4. Dissolve soybean protein nanofibers and sodium alginate separately in deionized water to obtain soybean protein nanofiber aqueous solution and sodium alginate aqueous solution. Then mix the soybean protein nanofiber aqueous solution, sodium alginate aqueous solution and egg yolk oil emulsion, and stir thoroughly to obtain mixed solution B. Use a peristaltic pump to drop mixed solution B into calcium chloride with a mass concentration of 0.1 mol / L at a rate of 1-2 rpm. After the addition is complete, soak for 30-50 minutes, wash with deionized water 3-4 times, and freeze dry to obtain egg yolk oil hydrogel beads.

6. The children's skin soothing and repairing cream containing egg yolk oil extract according to claim 5, characterized in that, The mass ratio of deionized water, compound emulsifier, and egg yolk oil extract in step B1 is 90-105:1.9-3.2:5.5-8; The composite emulsifier is obtained by mixing soy protein isolate and Tween 80 at a mass ratio of 2-3:

1.

7. The children's skin soothing and repairing cream containing egg yolk oil extract according to claim 5, characterized in that, The water bath heating in step B3 is performed at a temperature of 80-88°C for 10-12 hours.

8. The children's skin soothing and repairing cream containing egg yolk oil extract according to claim 5, characterized in that, The mass fraction of the soybean protein nanofiber aqueous solution in step B4 is 1%-2%; The sodium alginate aqueous solution has a mass fraction of 1%-2%; The volume ratio of the soybean protein nanofiber aqueous solution, sodium alginate aqueous solution, and egg yolk oil emulsion is 70-80:20-30:1-2.

9. The children's skin soothing and repairing cream containing egg yolk oil extract according to claim 1, characterized in that, The plant extract was obtained by mixing purslane extract, saffron extract, quinoa seed extract, artemisia capillaris extract and chlorella extract in a mass ratio of 5-8:2.2-4.7:2.2-4.7:1-2:1-2; Phase A comprises the following raw materials in parts by weight: Propylene glycol 12-16 parts; nicotinamide 1-2 parts; isopentyl glycol 0.5-1 part; allantoin 0.1-0.2 parts; sodium gluconate 0.01-0.05 parts; Octanoyl hydroxamic acid 0.05-0.1 parts; polyquaternium salt 1-2 parts; p-hydroxyacetophenone 0.1-0.3 parts; carbomer 0.1-0.3 parts; deionized water 74-79 parts; Phase B comprises the following raw materials in parts by weight: Egg yolk oil extract 2-4 parts; patchouli leaf extract 0.5-0.8 parts; chamomile flower extract 0.3-0.5 parts; licorice root extract 0.0001-0.001 parts; centella asiatica root extract 0.0001-0.001 parts; peony seed oil 0.5-0.8 parts; sunflower seed oil 0.5-0.8 parts; squalane 2-4 parts; Bisabolol 0.3-0.6 parts; Shea butter 3-6 parts; Glyceryl stearate 1-3 parts; Cetearyl alcohol 1-3 parts; Polydimethylsiloxane 1-3 parts; Tocopherol 1-3 parts; The C phase comprises the following raw materials in parts by weight: Chlorhexidine digluconate 1-2 parts.

10. The method for preparing a children's skin soothing and repairing cream containing egg yolk oil extract according to any one of claims 1-9, characterized in that, Includes the following steps: Step S1. Add deionized water to a water pot, add phase A raw material while stirring, heat and maintain the temperature at 82-90°C, stir for 30-50 minutes, and keep warm to obtain mixture 1; Step S2. Add phase B raw materials to the oil pot in sequence, and heat to 75-80°C while stirring. After complete dissolution, keep warm for later use to obtain mixture 2. Step S3. Mixture 1 is vacuum-inhaled into an emulsification pot, then mixture 2 is added, and the mixture is continuously stirred at 82-88°C for 5-8 minutes. After vacuum degassing for 10-15 minutes, the mixture is cooled to 35-40°C and then the C phase raw material is added to obtain the auxiliary material. Step S4. Add egg yolk oil hydrogel beads, silk fibroin microspheres and plant extracts to the excipients at 35-40°C, stir well, and you will get the children's skin soothing and repairing cream.

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