Microfluidic electrochemical detection device

Abstract

The present disclosure relates to the technical field of electrochemical detection, and discloses a microfluidic electrochemical detection device, which comprises a base, a channel plate, a filter device, a glass cover plate and an electrode plate, wherein the base comprises an electrode groove; the channel plate is arranged on the base and comprises a liquid inlet channel, a reaction chamber and a liquid outlet channel which are in communication with each other; the reaction chamber has a first open end and a second open end which are in communication; the liquid inlet channel comprises a first flow channel and a second flow channel, and the second flow channel is in communication with the reaction chamber; the end of the liquid outlet channel close to the reaction chamber has a liquid discharge port; in the axial direction of the liquid outlet channel, the cross-sectional area of the liquid discharge port gradually increases from the side away from the reaction chamber to the side close to the reaction chamber; the filter device is embedded in the channel plate and located between the first flow channel and the second flow channel; the glass cover plate is in sealing connection with the first open end; and the electrode plate is embedded in the electrode groove and in sealing connection with the second open end. The detection device has high detection precision and high accuracy of detection results.

Description

Technical Field

[0001] This disclosure relates to the field of electrochemical detection technology, and more specifically, to a microfluidic electrochemical detection device. Background Technology

[0002] Electrochemical detection, with its advantages of fast response, high sensitivity, small size, low cost, and portability, has been widely used in fields such as biomedicine, food safety, environmental monitoring, and industrial detection, enabling rapid quantitative analysis of substances such as blood glucose, uric acid, lactic acid, heavy metals, pesticide residues, veterinary drug residues, and biological antigens and antibodies. However, the detection accuracy of existing electrochemical detection devices is relatively low.

[0003] It should be noted that the information disclosed in the background section above is only used to enhance the understanding of the background of this disclosure, and therefore may include information that does not constitute prior art known to those skilled in the art. Summary of the Invention

[0004] This disclosure provides a microfluidic electrochemical detection device that can improve detection accuracy and the precision of detection results.

[0005] According to one aspect of this disclosure, a microfluidic electrochemical detection device is provided, comprising: Base, including electrode slots; A channel plate, fixed to the base, includes an interconnected inlet channel, a reaction chamber, and an outlet channel. The reaction chamber has a first open end and a second open end, which are distributed along a direction perpendicular to the surface of the base, with the first open end located on the side of the second open end away from the base. The inlet channel includes a first flow channel and a second flow channel, with the second flow channel communicating with the reaction chamber. The outlet channel has a drain port at its end near the reaction chamber. Along the axial direction of the outlet channel, and in the direction from the side away from the reaction chamber to the side near the reaction chamber, the cross-sectional area of ​​the drain port gradually increases. A filter device is embedded in the channel plate and located between the first flow channel and the second flow channel. The filter device can filter the test liquid input through the first flow channel. The test liquid after being filtered by the filter device is input into the reaction chamber through the second flow channel. A glass cover is located on the side of the reaction chamber away from the base and is sealed to the first open end; The electrode plate is embedded in the electrode groove and is sealed to the second open end.

[0006] In an exemplary embodiment of this disclosure, the filtration device includes an inlet pipe, a filter screen, and an outlet pipe. The inlet pipe is connected to the first flow channel, and the cross-sectional area of ​​the inlet pipe is larger than the cross-sectional area of ​​the first flow channel. The outlet pipe is connected to the second flow channel, and the filter screen is located between the inlet pipe and the outlet pipe.

[0007] In an exemplary embodiment of this disclosure, the end of the liquid inlet pipe near the filter screen is provided with a flange extending radially outward along the liquid inlet pipe, the flange having a protrusion protruding away from the filter screen, the protrusion engaging with the channel plate.

[0008] In one exemplary embodiment of this disclosure, the detection device further includes: The cover plate is detachably connected to the channel plate and can limit the position of the filter screen.

[0009] In one exemplary embodiment of this disclosure, the base further includes a positioning groove located at the bottom of the electrode groove. The orthographic projection of the electrode plate on the base covers the positioning groove, and the electrode plate is positioned through the positioning groove during assembly.

[0010] In one exemplary embodiment of this disclosure, the base further includes a fixing groove located within the electrode groove. An adsorption element is provided within the fixing groove, and the electrode plate is located on the adsorption element. The adsorption element can apply an adsorption force to the electrode plate.

[0011] In one exemplary embodiment of this disclosure, the material of the adsorption element is silicone.

[0012] In one exemplary embodiment of this disclosure, the glass cover is made of the same material as the channel plate.

[0013] In one exemplary embodiment of this disclosure, the detection device further includes: A gasket is located between the base and the channel plate and is aligned with the second open end of the reaction chamber; at least a portion of the gasket is located on the electrode plate and is sealed to both the second open end and the edge region of the electrode plate.

[0014] In one exemplary embodiment of this disclosure, the washer is made of rubber.

[0015] The microfluidic electrochemical detection device disclosed herein can precisely accommodate and limit the electrode plate through the electrode groove, which helps to reduce the probability of horizontal displacement or rotation of the electrode plate during detection and ensures the alignment accuracy between the reaction chamber and the electrode plate. During use, the liquid to be tested flows through the inlet channel through the filter device, then enters the reaction chamber, and after detection, it is discharged through the outlet channel, realizing continuous inflow and outflow of the liquid to be tested and achieving dynamic continuous detection. In the above process, the filter device can effectively intercept suspended particulate matter, biological tissue fragments, polymers, colloidal impurities, etc. in the liquid to be tested, preventing impurities from adhering to the electrode plate surface after entering the reaction chamber, causing problems such as electrode passivation, reduced response current, and signal drift, which helps to extend the service life of the electrode plate and improve the reliability of the detection signal. The glass cover is sealed to the first open end of the reaction chamber. This seals the top of the chamber, preventing evaporation of the test liquid and external contamination. It also provides an observation window for real-time monitoring of the liquid's filling status, bubble formation, and reaction process. Simultaneously, the glass surface fully wets the test liquid, aiding in the removal of residual air from the reaction chamber and preventing bubble interference, thus improving detection accuracy. The electrode plate is embedded in the electrode slot and sealed to the second open end, forming a bottom seal. This makes the reaction chamber a closed cavity connected only by the inlet and outlet channels, ensuring precise liquid quantification, eliminating the risk of leakage, and reducing detection accuracy deviations caused by evaporation or leakage of the test liquid. This further improves detection precision and the accuracy of the results.

[0016] In addition, the drain port can serve as the connection port between the liquid outlet channel and the reaction chamber, employing a tapered or flared structure with a gradually decreasing cross-sectional area along the liquid flow direction. The liquid within the reaction chamber can directly contact the lower surface of the glass cover at the drain port. Utilizing the excellent wetting properties of the glass, it spreads rapidly, pushing air from the drain port towards the liquid outlet channel for smooth discharge, achieving complete bubble-free filling of the reaction chamber. This bubble-free environment ensures the electrode plate surface is completely covered by liquid, guaranteeing a smooth electron transfer path and stable detection signals.

[0017] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and are not intended to limit this disclosure. Attached Figure Description

[0018] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments consistent with this disclosure and, together with the description, serve to explain the principles of this disclosure. It is obvious that the drawings described below are merely some embodiments of this disclosure, and those skilled in the art can obtain other drawings based on these drawings without any inventive effort.

[0019] Figure 1This is a schematic diagram of a microfluidic electrochemical detection device in one embodiment of the present disclosure.

[0020] Figure 2 This is a top view of the channel plate, fastening device and filtering device after assembly in one embodiment of this disclosure.

[0021] Figure 3 This is a schematic diagram of the electrode groove, positioning groove, and fixing groove in one embodiment of the present disclosure.

[0022] Figure 4 This is a schematic diagram of an electrode plate in one embodiment of the present disclosure.

[0023] Figure 5 As shown in one embodiment of this disclosure Figure 3 A cross-sectional view taken along the aa direction.

[0024] Figure 6 This is a top view of the base, channel plate, fastening device, limiting member and filtering device after assembly in one embodiment of this disclosure.

[0025] Figure 7 This is a schematic diagram of a pressure ring, a threaded interface, and a tapered connector in one embodiment of the present disclosure.

[0026] Figure 8 This is a schematic diagram of a filtering device in one embodiment of the present disclosure.

[0027] Figure 9 This is a top view of the inlet channel, reaction chamber, drain port, and outlet channel in one embodiment of the present disclosure.

[0028] Figure 10 This is a schematic diagram showing the distribution of multiple reaction chambers on the same channel plate and the liquid inlet and liquid outlet channels connected to each reaction chamber in one embodiment of this disclosure.

[0029] Figure 11 This is a schematic diagram showing the distribution of multiple reaction chambers on the same channel plate and the liquid inlet and liquid outlet channels connected to each reaction chamber in another embodiment of this disclosure.

[0030] Figure 12 This is a schematic diagram showing the distribution of multiple reaction chambers on the same channel plate and the liquid inlet and liquid outlet channels connected to each reaction chamber in another embodiment of this disclosure.

[0031] Figure 13 This is a schematic diagram of the base and channel plate assembled in one embodiment of this disclosure.

[0032] Figure 14 This is a schematic diagram of the base and channel plate assembled in another embodiment of this disclosure.

[0033] Explanation of reference numerals in the attached figures: 1. Base; 101. Electrode groove; 102. Positioning groove; 103. Fixing groove; 104. Adsorption component; 2. Channel plate; 21. Liquid inlet channel; 211. First flow channel; 212. Second flow channel; 22. Reaction chamber; 23. Liquid outlet channel; 231. Drain outlet; 24. Liquid inlet; 25. Liquid outlet; 26. Deformation space; 3. Glass cover plate; 4. Electrode plate; 41. Working electrode; 42. Counter electrode; 43. Reference electrode; 44. Substrate; 5. Filtration device; 51. Liquid inlet pipe; 52. Filter screen; 53. Liquid outlet pipe; 54. Flange; 55. Protrusion; 56. Cover plate; 6. Washer; 61. Raised ring; 7. Fastening device; 71. Bolt; 72. Nut; 81. Pressure ring; 82. Threaded interface; 83. Inverted conical connector; 9. Liquid inlet pipe; 10. Limiting wall; 11. Pressure plate. Detailed Implementation

[0034] Exemplary embodiments will now be described more fully with reference to the accompanying drawings. However, these exemplary embodiments can be implemented in many forms and should not be construed as limited to the embodiments set forth herein; rather, they are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the exemplary embodiments to those skilled in the art. The same reference numerals in the drawings denote the same or similar structures, and therefore detailed descriptions of them will be omitted. Furthermore, the drawings are merely illustrative of this disclosure and are not necessarily drawn to scale.

[0035] Although relative terms such as "up" and "down" are used in this specification to describe the relative relationship of one component of an icon to another, these terms are used only for convenience, such as according to the orientation of the examples shown in the accompanying drawings. It is understood that if the device of the icon is flipped upside down, the component described as "up" will become the component described as "down." When a structure is "up" of another structure, it may mean that the structure is integrally formed on the other structure, or that the structure is "directly" mounted on the other structure, or that the structure is "indirectly" mounted on the other structure through another structure.

[0036] The terms “a,” “one,” “the,” “the,” and “at least one” are used to indicate the presence of one or more elements / components / etc.; the terms “including” and “having” are used to indicate an open-ended inclusion and to mean that there may be other elements / components / etc. in addition to the listed elements / components / etc.; the terms “first” and “second” are used only as markers and are not a limitation on the number of objects.

[0037] This disclosure provides a microfluidic electrochemical detection device, which can be widely used in fields such as blood glucose detection, uric acid detection, lactic acid detection, heavy metal ion detection, pesticide residue detection, veterinary drug residue detection, biological antigen and antibody detection, water quality testing, and food additive detection. It is suitable for various scenarios including precise laboratory testing, rapid on-site testing, and batch sample screening. Figure 1 and Figure 2 As shown, the detection device includes a base 1, a channel plate 2, a glass cover plate 3, an electrode plate 4, and a filter device 5, wherein: The base 1 includes an electrode groove 101; The channel plate 2 is fixed on the base 1. The channel plate 2 includes an inlet channel 21, a reaction chamber 22, and an outlet channel 23 that are interconnected. The reaction chamber 22 has a first open end and a second open end that are connected through each other. The first open end and the second open end are distributed in a direction perpendicular to the surface of the base 1, and the first open end is located on the side of the second open end away from the base 1. The inlet channel 21 includes a first flow channel 211 and a second flow channel 212, and the second flow channel 212 is connected to the reaction chamber 22. The outlet channel 23 has a drain port 231 at the end near the reaction chamber 22. In the axial direction of the outlet channel 23, and in the direction from the side away from the reaction chamber 22 to the side near the reaction chamber 22, the cross-sectional area of ​​the drain port 231 gradually increases. The filter device 5 is embedded in the channel plate 2 and located between the first flow channel 211 and the second flow channel 212. The filter device 5 can filter the test liquid input through the first flow channel 211. The test liquid after being filtered by the filter device 5 is input into the reaction chamber 22 through the second flow channel 212. The glass cover 3 is located on the side of the reaction chamber 22 away from the base 1 and is sealed to the first open end; The electrode plate 4 is embedded in the electrode groove 101 and is sealed to the second open end.

[0038] The microfluidic electrochemical detection device disclosed herein can precisely accommodate and limit the electrode plate 4 through the electrode groove 101, which helps to reduce the probability of horizontal displacement or rotation of the electrode plate 4 during detection and ensures the alignment accuracy between the reaction chamber 22 and the electrode plate 4. During use, the liquid to be tested can flow through the inlet channel 21 through the filter device 5 and then enter the reaction chamber 22. After detection, it can be discharged through the outlet channel 23, realizing continuous inflow and outflow of the liquid to be tested and achieving dynamic continuous detection. In the above process, the filter device 5 can effectively intercept suspended particulate matter, biological tissue fragments, polymers, colloidal impurities, etc. in the liquid to be tested, preventing impurities from entering the reaction chamber 22 and adhering to the surface of the electrode plate 4, causing problems such as electrode passivation, reduced response current, and signal drift. This helps to extend the service life of the electrode plate 4 and improve the reliability of the detection signal. The glass cover 3 is sealed to the first open end of the reaction chamber 22. This seals the top of the reaction chamber 22, preventing the liquid to be tested from evaporating and becoming contaminated. It also provides an observation window for real-time monitoring of the liquid's filling status, bubble formation, and the reaction process. Simultaneously, the glass surface fully wets the liquid, helping to expel residual air from the reaction chamber 22 and preventing bubble interference, thus improving detection accuracy. The electrode plate 4 is embedded in the electrode groove 101 and sealed to the second open end, forming a bottom seal. This makes the reaction chamber 22 a closed cavity connected only to the liquid inlet channel 21 and the liquid outlet channel 23. This ensures accurate liquid metering, eliminates the risk of leakage, and reduces detection accuracy deviations caused by liquid evaporation or leakage, further improving detection precision and the accuracy of the results.

[0039] In addition, the drain port 231 can serve as the connection port between the liquid outlet channel 23 and the reaction chamber 22. It adopts a tapered or horn-shaped structure, with the cross-sectional area gradually decreasing along the liquid flow direction. The liquid in the reaction chamber 22 can directly contact the lower surface of the glass cover plate 3 at the drain port 231. At this time, the good wetting properties of the glass can be used to spread it quickly, pushing the air in the reaction chamber 22 from the drain port 231 to the liquid outlet channel 23 and smoothly expelling it, thus achieving complete bubble-free filling of the reaction chamber 22. The bubble-free environment ensures that the surface of the electrode plate 4 is completely covered by liquid, the electron transfer path is unobstructed, and the detection signal is stable.

[0040] The following provides a detailed description of each part and specific details of the microfluidic electrochemical detection device disclosed herein: The base 1 can be plate-shaped, serving as the supporting base for the detection device of this disclosure. Its material can be plastic, resin, or plexiglass, etc. Figure 3 As shown, the base 1 may include an electrode groove 101, which may be a groove structure formed on the upper surface of the base 1. The shape of the electrode groove 101 may be rectangular, circular, elliptical, polygonal or irregular, and no special limitation is made here.

[0041] Please continue reading Figure 1 As shown, the channel plate 2 can be fixed above the base 1. For example, the channel plate 2 and the base 1 can be detachably connected by a fastening device 7. In an exemplary embodiment of this disclosure, the fastening device 7 may include a bolt 71 and two nuts 72. The bolt 71 can pass through the channel plate 2 and the base 1. One nut 72 can be threaded to the outer periphery of the end of the bolt 71 away from the base 1. At the same time, the other nut 72 can be threaded to the outer periphery of the end of the bolt 71 away from the channel plate 2 and can be embedded in the base 1.

[0042] The material of the channel plate 2 can be transparent resin, transparent polymethyl methacrylate (PMMA), or polydimethylsiloxane (PDMS), etc., possessing good light transmittance and chemical stability, facilitating the fabrication of microchannels and chamber structures. The channel plate 2 may include interconnected inlet 24, inlet channel 21, reaction chamber 22, outlet channel 23, and outlet 25. The inlet 24, inlet channel 21, reaction chamber 22, outlet channel 23, and outlet 25 can be integrally formed inside the channel plate 2, interconnected without dead corners. The inner walls of the channels (such as inlet channel 21 and outlet channel 23) are smooth, which can reduce the flow resistance of the liquid to be measured and reduce the adhesion of air bubbles.

[0043] In one exemplary embodiment of this disclosure, the inlet 24 can be used to connect to an external liquid injection device, such as a syringe pump, peristaltic pump, pipette, etc., to enable automatic or manual introduction of the liquid to be tested. The reaction chamber 2 can be a cylindrical or elliptical cylindrical chamber, which may have a through first open end and a second open end. The first open end and the second open end are distributed along a direction perpendicular to the surface of the base 1, and the first open end is located on the side of the second open end away from the base 1. That is, compared to the base 1, the first open end faces upward and the second open end faces downward.

[0044] The glass cover 3 can be a transparent glass sheet with a smooth and clean surface, possessing good wetting properties and chemical stability. The glass cover 3 is located on the side of the reaction chamber 22 away from the base 1 and is sealed to the first open end. That is, the top of the reaction chamber 22 can be sealed by the glass cover 3, preventing the evaporation of the test liquid within the reaction chamber 22 and avoiding the entry of external dust and impurities that could contaminate the test liquid. The glass cover 3 also provides a clear observation window for real-time monitoring of the filling, bubble removal, and reaction progress of the test liquid. The surface of the glass cover 3 has good wetting properties with the test liquid, allowing it to spread rapidly upon contact with the surface of the glass cover 3, facilitating the pushing of air within the chamber towards the liquid outlet channel 23, achieving bubble-free filling.

[0045] Electrode plate 4 can be an electrode used for electrochemical detection, such as... Figure 4 As shown, the electrode plate 4 may include a working electrode 41, a counter electrode 42, a reference electrode 43, and a substrate 44. The working electrode 41, counter electrode 42, and reference electrode 43 can all be formed on the surface of the substrate 44. For example, the working electrode 41, counter electrode 42, and reference electrode 43 can be formed on the surface of the substrate 44 using a screen printing process. The working electrode 41 may be provided with specific antibodies, aptamers, enzymes, or other biosensitive elements capable of reacting with the test liquid to achieve specific detection of the target analyte. The electrode plate 4 can be embedded in the electrode groove 101 of the base 1, and the shape of the electrode groove 101 matches the shape of the electrode plate 4. For example, when the electrode plate 4 is rectangular, the electrode groove 101 can be a rectangular groove; when the electrode plate 4 is circular, the electrode groove 101 can be a circular groove. The depth of the electrode groove 101 can match the thickness of the electrode plate 4; for example, the depth of the electrode groove 101 can be equal to the thickness of the electrode plate 4.

[0046] The upper surface of electrode plate 4 can be sealed to the second open end of reaction chamber 22. Under the joint sealing of glass cover plate 3 and electrode plate 4, reaction chamber 22 can form an independent quantitative reaction chamber with a fixed volume, enabling micro-quantitative detection at the nano-level and micro-level with a volume error of less than 0.1 μL. This ensures the consistency of the reaction system for each detection, which helps to improve the repeatability and accuracy of the detection results.

[0047] In one exemplary embodiment of this disclosure, please continue to refer to Figure 3 As shown, the base 1 also includes a positioning groove 102, which can be located at the bottom of the electrode groove 101, for example, it can be located in the central area of ​​the electrode groove 101. During the assembly of the electrode plate 4, the positioning groove 102 can be used for positioning to prevent the electrode plate 4 from being misaligned, which helps to improve the installation accuracy of the electrode plate 4.

[0048] In one exemplary embodiment of this disclosure, please continue to refer to Figure 3 As shown, the base 1 may also include a fixing groove 103, which is located at the bottom of the electrode groove 101, and the fixing groove 103 may be distributed side by side with the positioning groove 102. Figure 5 As shown, an adsorption member 104 may be provided in the fixing groove 103, and the electrode plate 4 is located on the adsorption member 104. The adsorption member 104 can apply an adsorption force to the electrode plate 4 to fix the position of the electrode plate 4 and prevent the electrode plate 4 from shifting or shaking during the detection process. In an exemplary embodiment of this disclosure, the material of the adsorption member 104 is silicone.

[0049] In one exemplary embodiment of this disclosure, please continue to refer to Figure 1 , Figure 2 and Figure 6As shown, the liquid inlet channel 21 may include a first flow channel 211 and a second flow channel 212, wherein the second flow channel 212 is connected to the reaction chamber 22; the detection device may also include a filter device 5, which may be embedded in the channel plate 2 and located between the first flow channel 211 and the second flow channel 212. The filter device 5 can filter the test liquid input through the first flow channel 211, and the test liquid after being filtered by the filter device 5 can be input into the reaction chamber 22 through the second flow channel 212.

[0050] The first flow channel 211 can be the initial liquid inlet section, and its cross-section can be circular, elliptical, rectangular, or polygonal. One end of the first flow channel 211 can extend to the surface of the channel plate 2 away from the base 1, thereby forming a liquid inlet 24 on that surface, such as... Figure 7 As shown, the liquid inlet 24 can be connected to the liquid inlet pipe 9 via a pressure ring 81, a threaded interface 82, and a tapered connector 83, so that the liquid to be tested can be input through the liquid inlet pipe 9. The other end of the first flow channel 211 can be connected to the filter device 5. The second flow channel 212 can serve as a transport section for the filtered liquid to be tested, with one end connected to the filter device 5 and the other end connected to the reaction chamber 22.

[0051] like Figure 8 As shown, the filter device 5 can be integrally embedded in the pre-set mounting cavity inside the channel plate 2, without occupying external space, resulting in a compact structure. The filter device 5 effectively intercepts suspended particulate matter, biological tissue fragments, polymers, colloidal impurities, etc., in the test liquid, preventing impurities from entering the reaction chamber 22 and adhering to the surface of the electrode plate 4, thus avoiding problems such as electrode passivation, reduced response current, and signal drift. Filtration extends the service life of the electrode plate 4, ensures stable and continuous electrochemical reaction, and improves the reliability of the detection signal.

[0052] In one exemplary embodiment of this disclosure, please continue to refer to Figure 8 As shown, the filtration device 5 may include an inlet pipe 51, a filter screen 52, and an outlet pipe 53. The inlet pipe 51 is connected to the first flow channel 211, and the cross-sectional area of ​​the inlet pipe 51 is larger than that of the first flow channel 211. The outlet pipe 53 is connected to the second flow channel 212. The filter screen 52 is located between the inlet pipe 51 and the outlet pipe 53. The inlet pipe 51 can serve as a buffer section for the liquid before filtration. Its cross-sectional area is larger than that of the first flow channel 211, which increases the contact area between the liquid to be tested and the filter screen 52, thereby improving filtration efficiency.

[0053] The filter screen 52 can be a porous membrane structure, and its material can be nylon, polytetrafluoroethylene, mixed cellulose ester, etc. The edge of the filter screen 52 can be fixed between the liquid inlet pipe 51 and the liquid outlet pipe 53 to ensure that all liquid passes through the filter screen 52.

[0054] In one exemplary embodiment of this disclosure, please continue to refer to Figure 8 As shown, the end of the liquid inlet pipe 51 near the filter screen 52 is provided with a flange 54 extending radially outward along the liquid inlet pipe 51. The flange 54 has a protrusion 55 protruding away from the filter screen 52, and the protrusion 55 can be engaged with the channel plate 2. The flange 54 can extend radially outward along the liquid inlet pipe 51, which can increase the contact area between the filter device 5 and the mounting cavity of the channel plate 2, and improve the assembly firmness. The protrusion 55 can be a block-shaped or annular protrusion structure, which can engage with the pre-set slots or recesses on the channel plate 2 to achieve detachable fixing.

[0055] In one exemplary embodiment of this disclosure, please continue to refer to Figure 8 As shown, the detection device may further include a cover plate 56, which is detachably connected to the channel plate 2 and can limit the position of the filter screen 52. For example, the cover plate 56 is located above the filter device 5 and can cover the installation area of ​​the filter screen 52. The cover plate 56 can be detachably connected to the channel plate 2 by means of threads, snaps, magnets, etc. The lower surface of the cover plate 56 can contact the edge of the filter screen 52, and can apply uniform pressure to the filter screen 52, thereby firmly pressing the filter screen 52 between the inlet pipe 51 and the outlet pipe 53. In this disclosure, the filter screen 52 can also be replaced by removing the cover plate 56, and the cover plate 56 can be installed after replacement.

[0056] In one exemplary embodiment of this disclosure, such as Figure 9 As shown, the end of the liquid outlet channel 23 near the reaction chamber 22 has a drain port 231; in the axial direction of the liquid outlet channel 23, and in the direction from the side away from the reaction chamber 22 to the side near the reaction chamber 22, the cross-sectional area of ​​the drain port 231 gradually increases.

[0057] In this disclosure, the drain port 231 can be the connection port between the liquid outlet channel 23 and the reaction chamber 22, and adopts a tapered or horn-shaped structure with a gradually decreasing cross-sectional area along the liquid flow direction. In an exemplary embodiment of this disclosure, the top of the drain port 231 is higher than or flush with the upper surface of the reaction chamber 22, allowing the liquid to be tested in the reaction chamber 22 to directly contact the lower surface of the glass cover plate 3, and then quickly spread along the lower surface of the glass cover plate 3, pushing the air in the reaction chamber 22 from the drain port 231 to the liquid outlet channel 23 and smoothly discharging it, thus achieving complete bubble-free filling of the reaction chamber 22. The bubble-free environment ensures that the surface of the electrode plate 4 is completely covered by liquid, the electron transfer path is unobstructed, and the detection signal is stable.

[0058] In one exemplary embodiment of this disclosure, please continue to refer to Figure 1As shown, the detection device also includes a gasket 6, which can be made of a flexible annular seal, for example, rubber; such as silicone rubber, fluororubber, nitrile rubber, etc., possessing good elasticity, sealing performance, and chemical stability. The gasket 6 is located between the base 1 and the channel plate 2, and is aligned with the second open end of the reaction chamber 22; at least a portion of the gasket 6 is located on the electrode plate 4, and is sealed to both the second open end and the edge region of the electrode plate 4. At this time, the working electrode 41, the counter electrode 42, and the reference electrode 43 in the electrode plate 4 are all located within the central region of the gasket 6.

[0059] In some embodiments of this disclosure, the lower surface of the washer 6 can be pressed against the edge region of the electrode plate 4 (i.e., the substrate 44 surrounding the working electrode 41, the counter electrode 42, and the reference electrode 43). It should be noted that... Figure 2 , Figure 3 and Figure 6 The area indicated by the dashed circle is the region where gasket 6 is located after installation. The upper surface of gasket 6 can be tightly fitted to the second open end of reaction chamber 22 to form a sealed structure. The hollow area in gasket 6 can communicate with reaction chamber 22. The liquid to be tested can first fill the hollow area in gasket 6 and then fill reaction chamber 22. This expands the volume of reaction chamber 22 through gasket 6, ensuring sufficient measurement of the liquid to be tested. At the same time, it can effectively prevent the liquid to be tested from leaking from the edges of reaction chamber 22, ensuring a constant volume of reaction chamber 22 and accurate quantification.

[0060] In one exemplary embodiment of this disclosure, such as Figures 10-12 As shown, the channel plate 2 can be equipped with multiple reaction chambers 22. Each reaction chamber 22 can be equipped with a liquid inlet channel 21 and a liquid outlet channel 23. Each reaction chamber 22 can also be equipped with an electrode plate 4 (the rectangle shown by the dashed line in the figure indicates the location of the electrode plate 4). Additionally, each reaction chamber 22 can be equipped with a glass cover plate 3. Simultaneous detection through multiple reaction chambers 22 helps improve detection efficiency.

[0061] Please refer to some embodiments of this disclosure. Figure 10 As shown, the liquid inlet channels 21 corresponding to different reaction chambers 22 are independently distributed, and each liquid inlet channel 21 can be provided with a corresponding liquid inlet 24; at the same time, the liquid outlet channels 23 corresponding to different reaction chambers 22 are also independently distributed, and each liquid outlet channel 23 can be provided with a corresponding liquid outlet 25. In other embodiments of this disclosure, please continue to refer to... Figure 11 As shown, the liquid inlet channels 21 corresponding to different reaction chambers 22 in the same channel plate 2 can be connected to the same liquid inlet 24, allowing the liquid to be tested to be simultaneously supplied to multiple reaction chambers 22 through one liquid inlet 24. In further embodiments of this disclosure, please refer to... Figure 12As shown, the liquid inlet channels 21 corresponding to different reaction chambers 22 in the same channel plate 2 can be connected to the same liquid inlet 24, and the liquid to be tested can be delivered to multiple reaction chambers 22 simultaneously through one liquid inlet 24; at the same time, the liquid outlet channels 23 corresponding to different reaction chambers 22 in the same channel plate 2 can be connected to the same liquid outlet 25, and the liquid in multiple reaction chambers 22 can be discharged through one liquid outlet 25, which helps to improve the liquid discharge rate.

[0062] The working principle of the microfluidic electrochemical detection device disclosed herein will be explained in detail below: Before testing, electrode plate 4 is embedded into electrode groove 101 in base 1, with the bottom surface of electrode plate 4 in contact with adsorption element 104 and firmly adsorbed and positioned. The area where working electrode 41 is located is aligned with positioning groove 102. Gasket 6 is placed on base 1 and surrounds the outer periphery of working electrode 41, counter electrode 42, and reference electrode 43 in electrode plate 4. Channel plate 2 is fixed to base 1, and the second open end of reaction chamber 22 in channel plate 2 is aligned with gasket 6. Channel plate 2 can press gasket 6 tightly, thereby achieving a seal between base 1 and channel plate 2. Subsequently, glass cover plate 3 can be sealed to the first open end of reaction chamber 22 to form a top seal.

[0063] During testing, an external liquid injection device introduces the test liquid into the filter device 5 through the first flow channel 211 of the inlet channel 21. The filter screen 52 in the filter device 5 filters impurities in the test liquid to avoid affecting the test results and improve the accuracy of the results. The filtered test liquid enters the second flow channel 212 through the outlet pipe 53, and then flows into the reaction chamber 22 through the second flow channel 212. After entering the reaction chamber 22, the test liquid spreads rapidly upon contact with the lower surface of the glass cover plate 3, pushing the air in the reaction chamber 22 from the drain port 231 to the outlet channel 23 and expelling it, achieving complete filling without air bubbles. The test liquid fully contacts the surface of the electrode plate 4 in the reaction chamber 22, undergoing a specific electrochemical reaction. The electrode plate 4 transmits the electrical signal to the electrochemical workstation to realize the detection of the target substance concentration.

[0064] After the test is completed, the waste liquid can be discharged through the liquid outlet channel 23, and the cleaning liquid can be injected to clean the device. At the same time, the cover plate 56 can be removed to replace the filter screen 52, and the channel plate 2 can be removed to replace the electrode plate 4. The device disclosed herein is reusable, easy to maintain, and has low operating costs.

[0065] It should also be noted that the detection device disclosed herein can be used for real-time dynamic continuous monitoring of the liquid to be tested, that is, it can dynamically monitor the change in the concentration of the target substance (i.e., the substance that can specifically react with the electrode plate) in the liquid to be tested over time. For example, the liquid to be tested can be continuously introduced into the reaction chamber 22 through the liquid inlet channel 21. After the liquid to be tested passes through the reaction chamber 22, it can be discharged in real time through the liquid outlet channel 23. During this process, if the concentration of the target substance in the liquid to be tested introduced into the reaction chamber 2 at different times is different, the detection device can detect the change trend of the concentration of the target substance in the liquid to be tested over the detection time in real time, providing a basis for subsequent data analysis.

[0066] The manufacturing method and usage process of the detection device disclosed herein are described below through several embodiments: Example 1 Example 1 provides a method for fabricating a microfluidic electrochemical detection device with filtration function, as detailed below: The first step involves fabricating the main body of the channel plate 2 using photopolymer 3D printing. The main body of the channel plate 2 is made of photosensitive resin, and the inlet 24 has a threaded interface 82 above it. The inlet 24 can be connected to the inlet pipe 9 via a pressure ring 81, the threaded interface 82, and a tapered connector 83, as detailed below. Figure 7 As shown, this facilitates liquid injection. A threaded interface 82 is also provided above the outlet 25. The outlet 25 can be connected to the outlet pipe (not shown in the figure) via a pressure ring 81, the threaded interface 82, and the inverted conical connector 83, facilitating liquid discharge.

[0067] The second step is to embed the filter screen 52 into the channel plate 2 and attach the cover plate 56 to seal the filter screen 52.

[0068] The third step is to attach a glass cover plate 3 to the first open end of the reaction chamber 22 in the channel plate 2. For example, the glass cover plate 3 can be attached with resin adhesive.

[0069] The fourth step is to use hot melt 3D printing to make the base 1. During this process, electrode grooves 101, positioning grooves 102 and fixing grooves 103 can be formed on the base 1.

[0070] Fifth step: Inject absorbent silica gel into the fixing groove 103 to form the absorbent component 104.

[0071] Step 6, Assembly: First, place the electrode plate 4 in the electrode groove 101 of the base 1, aligning the working electrode 41 in the electrode plate 4 with the positioning groove 102, and adsorb the electrode plate 4 onto the adsorption component 104; then place the silicone gasket 6 on the electrode plate 4; then, move the channel plate 2 along the limiting wall 10 (e.g., Figure 3 and Figure 6As shown, the channel plate 2 is placed on the base 1, with a washer 6 and an electrode plate 4 sandwiched between the channel plate 2 and the base 1; finally, the two are clamped together by a fastening device 7. During this process, the channel plate 2 is pressed down by force, and the washer 6 is clamped and flattened to prevent leakage.

[0072] Instructions for use: Connect the other end of the inlet pipe 9 to the injection pump; connect the bottom end of the electrode plate 4 to the adapter, and then connect it to the electrochemical workstation; then start injecting the liquid until the liquid to be tested fills the entire space of the reaction chamber 22; finally, perform the test. After use, clean the inlet channel 21, reaction chamber 22, and outlet channel 23 of the channel plate 2 thoroughly to remove any residue, and replace the filter screen 52 as needed.

[0073] Example 2

[0074] The only difference between Example 2 and Example 1 is that there is no filter device 5 in Example 2. The other processes are similar to those in Example 1 and will not be described again here.

[0075] Example 3

[0076] Example 3 provides a method for fabricating a microfluidic electrochemical detection device, as detailed below: The first step involves fabricating the main body of the channel plate 2 using laser cutting and vacuum hot pressing. The main body of the channel plate 2 is made of transparent acrylic glass, and threaded interfaces 82 are bonded above the inlet 24 and outlet 25. The inlet 24 can be connected to the inlet pipe 9 via a pressure ring 81, threaded interface 82, and inverted conical connector 83 to facilitate liquid injection. A threaded interface 82 is also bonded above the outlet 25. The outlet 25 can be connected to the outlet pipe via a pressure ring 81, threaded interface 82, and inverted conical connector 83 to facilitate liquid discharge. Compared to Example 1, the inlet 24 and reaction chamber 22 are in a direct flow path, without a filter device 5. The top of the reaction chamber 22 is a transparent acrylic plate made of the same material as the main body of the channel plate 2, which can serve as a glass cover 3. The main body of the channel plate 2 has four through holes at its four corners.

[0077] The second step is to use hot melt 3D printing to make the base 1. During this process, electrode grooves 101, positioning grooves 102 and fixing grooves 103 can be formed on the base 1; the four corners of the base 1 have four through holes.

[0078] The third step is to inject absorbent silicone into the fixing groove 103 and install four sets of fastening device bolts 71 at the through hole.

[0079] Step 4, Assembly: Place electrode plate 4 in electrode groove 101, align the working electrode 41 in electrode plate 4 with positioning groove 102, and adsorb electrode plate 4 onto adsorption component 104; then place silicone gasket 6 on electrode plate 4; then move channel plate 2 along limiting wall 10 (e.g., Figure 3 and Figure 6As shown, the channel plate 2 is placed on the base 1, with a washer 6 and an electrode plate 4 sandwiched between the channel plate 2 and the base 1. Finally, the nut 72 is installed, and the channel plate 2 is pressed down, clamping and flattening the washer 6 to prevent leakage. The device is now complete. A cross-sectional schematic diagram of this device is attached. Figure 13 As shown.

[0080] Instructions for use: Connect the other end of the inlet pipe 9 to the injection pump; then connect the bottom end of the electrode plate 4 to the adapter, and then connect it to the electrochemical workstation; then start injecting the liquid until the liquid to be tested fills the entire space of the reaction chamber 22; finally, perform the test. After use, clean the inlet channel 21, reaction chamber 22, and outlet channel 23 of the channel plate 2 thoroughly to remove any residue.

[0081] Example 4

[0082] Example 4 provides a method for fabricating a four-channel microfluidic electrochemical detection device with a four-input, four-output configuration and filtration function, as detailed below: The first step is to fabricate the four-channel channel plate 2 main body using photopolymer 3D printing (e.g., Figure 10 As shown, the main body of the channel plate 2 is made of photosensitive resin, and threaded interfaces 82 are provided above all liquid inlets 24 and liquid outlets 25. The liquid inlets 24 can be connected to the liquid inlet pipe 9 via a pressure ring 81, threaded interfaces 82, and a conical connector 83 for easy liquid injection. The liquid outlets 25 can be connected to the liquid outlet pipe via a pressure ring 81, threaded interfaces 82, and a conical connector 83 for easy liquid discharge. A filter device 5 is provided between the liquid inlets 24 and the reaction chamber 22.

[0083] The second step is to embed a filter screen 52 into the channel plate 2, attach a cover plate 56 to seal the filter screen 52, and attach a glass cover plate 3 to the upper part of the reaction chamber 22.

[0084] The third step involves manufacturing the base 1 body using a CNC machine tool. During this process, four electrode slots 101, along with corresponding positioning slots 102 and fixing slots 103, are formed on the base 1. It should be noted that the dimensions of the four-channel channel plate 2 body and the base 1 body are larger than those of a single-channel body.

[0085] The fourth step is to inject absorbent silicone into the fixing groove 103 and install the bolts 71 of the six fastening devices 7 that pass through the base 1 and the channel plate 2.

[0086] Fifth step, assembly: First, place four electrode plates 4 on the base 1, align the working electrode 41 of the electrode plate 4 with the positioning groove 102, and adsorb the electrode plate 4 onto the adsorption component 104; then place the silicone gasket 6 on the electrode plate 4; then place the channel plate 2 along the limiting wall 10 on the base 1, with the gasket 6 and the electrode plate 4 sandwiched between the channel plate 2 and the base 1; finally, assemble the nut 72. During this process, the channel plate 2 is pressed down by force, and the gasket 6 is clamped and flattened to prevent leakage.

[0087] Instructions for use: Connect the other end of the inlet pipe 9 to the injection pump; connect the bottom end of the electrode plate 4 to the adapter, and then connect it to the electrochemical workstation; then start injecting liquid into each channel until the liquid to be tested fills the entire space of the reaction chamber 22; finally, perform the test. After use, clean the inlet channel 21, reaction chamber 22 and outlet channel 23 of the channel plate 2 thoroughly to remove any residue, and replace the filter screen 52 as needed.

[0088] Example 5

[0089] Example 5 provides a method for fabricating a four-channel microfluidic electrochemical detection device with one inlet and four outlets and a filtration function, as detailed below: The first step is to fabricate the four-channel channel plate 2 main body using photopolymer 3D printing (e.g., Figure 11 As shown), the main body of the channel plate 2 is made of photosensitive resin, and includes one inlet 24 and four outlets 25. The connection method between the inlet 24 and the inlet pipe 9 and the connection method between the outlets 25 and the outlet pipes are similar to those in Example 1. A filter device 5 is located between the inlet 24 and the reaction chamber 22.

[0090] The second step is to embed a filter screen 52 into the channel plate 2, attach a cover plate 56 to seal the filter screen 52; and attach a glass cover plate 3 to the reaction chamber 22.

[0091] The third step is to manufacture the main body of the base 1 using injection molding. During this process, four electrode grooves 101, as well as positioning grooves 102 and fixing grooves 103 corresponding to each electrode groove 101, can be formed on the base 1.

[0092] The fourth step is to inject absorbent silicone into the fixing groove 103 and install the bolts 71 of the six fastening devices 7 that pass through the base 1 and the channel plate 2.

[0093] Fifth step, assembly: First, place four electrode plates 4 on the base 1, align the working electrode 41 of the electrode plate 4 with the positioning groove 102, and adsorb the electrode plate 4 onto the adsorption component 104; then place the silicone gasket 6 on the electrode plate 4; then place the channel plate 2 along the limiting wall 10 on the base 1, with the gasket 6 and the electrode plate 4 sandwiched between the channel plate 2 and the base 1; finally, assemble the nut 72. During this process, the channel plate 2 is pressed down by force, and the gasket 6 is clamped and flattened to prevent leakage.

[0094] Instructions for use: Connect the other end of the inlet pipe 9 to the injection pump; then connect the bottom end of the electrode plate 4 to the adapter, and then connect it to the electrochemical workstation; then start injecting liquid at the inlet 24. After the liquid to be tested passes through the filter device 5, it fills the entire reaction chamber 22 along the T-shaped branch channel; finally, perform the test. After use, clean the inlet channel 21, reaction chamber 22, and outlet channel 23 of the channel plate 2 thoroughly to remove any residue, and replace the filter screen 52 as needed.

[0095] Example 6

[0096] This embodiment 6 provides a method for preparing a four-channel microfluidic electrochemical detection device with a filter function and a single inlet and single outlet, as detailed below: The first step is to fabricate the main body of the four-channel channel plate 2 using photopolymer 3D printing (as shown in the attached image). Figure 12 As shown, the main body of the channel plate 2 is made of photosensitive resin and includes an inlet 24 and an outlet 25. Both the inlet 24 and the outlet 25 have threaded interfaces 82. The connection method between the inlet 24 and the inlet pipe 9 and the connection method between the outlet 25 and the outlet pipe are similar to those in Example 1. A filter device 5 is located between the inlet 24 and the reaction chamber 22.

[0097] The second step is to embed a filter screen 52 into the channel plate 2, attach a cover plate 56 to seal the filter screen 52; and attach a glass cover plate 3 to the reaction chamber 22.

[0098] The third step is to manufacture the main body of the base 1 using injection molding. During this process, four electrode grooves 101, as well as positioning grooves 102 and fixing grooves 103 corresponding to each electrode groove 101, can be formed on the base 1.

[0099] The fourth step is to inject absorbent silicone into the fixing groove 103 and install the bolts 71 of the six fastening devices 7 that pass through the base 1 and the channel plate 2.

[0100] Fifth step, assembly: First, place four electrode plates 4 on the base 1, align the working electrode 41 of the electrode plate 4 with the positioning groove 102, and adsorb the electrode plate 4 onto the adsorption component 104; then place the silicone gasket 6 on the electrode plate 4; then place the channel plate 2 along the limiting wall 10 on the base 1, with the gasket 6 and the electrode plate 4 sandwiched between the channel plate 2 and the base 1; finally, assemble the nut 72. During this process, the channel plate 2 is pressed down by force, and the gasket 6 is clamped and flattened to prevent leakage.

[0101] Instructions for use: Connect the other end of the inlet pipe 9 to the injection pump; then connect the bottom end of the electrode plate 4 to the adapter, and then connect it to the electrochemical workstation; then start injecting liquid at the inlet 24. After the liquid to be tested passes through the filter device 5, it fills the entire reaction chamber 22 along the T-shaped branch channel; finally, perform the test. After use, clean the inlet channel 21, reaction chamber 22, and outlet channel 23 of the channel plate 2 thoroughly to remove any residue, and replace the filter screen 52 as needed.

[0102] Example 7

[0103] This embodiment 7 provides a method for preparing a microfluidic electrochemical detection device, as detailed below: The first step involves fabricating the flexible channel plate 2 body using a casting and oxygen plasma treatment method to bond and seal the flow channels. During this process, the outer edge of the bottom of the reaction chamber 22 has a raised ring 61 (e.g., Figure 14 As shown), 61 can function as a gasket 6; the connection between the liquid inlet channel 21 and the liquid outlet channel 23 and the reaction chamber 22 is provided with a widened deformation space 26 to prevent the material from deforming under pressure, which would cause a significant reduction in the cross-section of the liquid inlet channel 21 and / or the liquid outlet channel 23; in this embodiment, there is no need to provide a threaded interface 82 on the liquid inlet 24. The liquid inlet 24 and the reaction chamber 22 are a direct flow channel, without a filter device 5.

[0104] The second step involves preparing the pressure plate 11 by cutting, using transparent plexiglass as the material.

[0105] The third step is to use hot melt 3D printing to make the main body of the base 1. During this process, electrode grooves 101, positioning grooves 102 and fixing grooves 103 can be formed on the base 1.

[0106] The fourth step is to inject absorbent silicone into the fixing groove 103 and install the bolts 71 of the four fastening devices 7 that pass through the base 1 and the pressure plate 11.

[0107] Fifth step, assembly: First, place the electrode plate 4 on the base 1, align the working electrode 41 with the positioning groove 102, and adsorb the electrode plate 4 onto the adsorption component 104; then place the channel plate 2 along the limiting wall 10 on the base 1, with the convex ring 61 at the bottom of the channel plate 2 located around the working electrode 41 on the electrode plate 4; then place the pressure plate 11, assemble the nut 72 of the fastening bolt 71, press the nut 72 onto the pressure plate 11, and the pressure plate 11 presses the channel plate 2 tightly, the channel plate 2 is pressed down by force, and the convex ring 61 is clamped and flattened to prevent leakage; finally, insert the inlet pipe 9 into the inlet port 24 and the outlet pipe into the outlet port 25.

[0108] Instructions for use: First, connect the end of the inlet pipe 9 furthest from the inlet 24 to the injection pump; connect the bottom of the electrode plate 4 to the adapter and connect it to the electrochemical workstation; then start injecting the liquid until the reaction chamber 22 is completely filled with the liquid to be tested; finally, perform the test. After use, clean the inlet channel 21, reaction chamber 22, and outlet channel 23 of the channel plate 2 thoroughly to remove any residue.

[0109] Other embodiments of this disclosure will readily occur to those skilled in the art upon consideration of the specification and practice of the invention disclosed herein. This disclosure is intended to cover any variations, uses, or adaptations of this disclosure that follow the general principles of this disclosure and include common knowledge or customary techniques in the art not disclosed herein. The specification and examples are to be considered exemplary only, and the true scope and spirit of this disclosure are indicated by the appended claims.

Claims

1. A microfluidic electrochemical detection device, characterized in that, include: Base, including electrode slots; A channel plate, fixed to the base, is detachably connected to the base. The channel plate includes an interconnected inlet channel, a reaction chamber, and an outlet channel. The reaction chamber has a through first open end and a second open end, which are distributed along a direction perpendicular to the surface of the base, with the first open end located on the side of the second open end away from the base. The inlet channel includes a first flow channel and a second flow channel, with the second flow channel communicating with the reaction chamber. The outlet channel has a drain port at its end near the reaction chamber. Along the axial direction of the outlet channel, and in the direction from the side away from the reaction chamber to the side near the reaction chamber, the cross-sectional area of ​​the drain port gradually increases. The top of the drain port is higher than the upper surface of the reaction chamber. A filter device is embedded in the channel plate and located between the first flow channel and the second flow channel. The filter device can filter the test liquid input through the first flow channel. The test liquid after being filtered by the filter device is input into the reaction chamber through the second flow channel. A glass cover is located on the side of the reaction chamber away from the base and is sealed to the first open end; An electrode plate is embedded in the electrode groove and is sealed to the second open end; The filtration device includes an inlet pipe, a filter screen, and an outlet pipe. The inlet pipe is connected to the first flow channel, and the cross-sectional area of ​​the inlet pipe is larger than that of the first flow channel. The outlet pipe is connected to the second flow channel, and the filter screen is located between the inlet pipe and the outlet pipe. The inlet pipe has a flange extending radially outward at the end near the filter screen, and the flange has a protrusion protruding away from the filter screen, which engages with the channel plate. The cover plate is detachably connected to the channel plate and can limit the position of the filter screen.

2. The detection device according to claim 1, characterized in that, The base also includes a positioning groove located at the bottom of the electrode groove. The orthographic projection of the electrode plate on the base covers the positioning groove, and the electrode plate is positioned through the positioning groove during assembly.

3. The detection device according to claim 1, characterized in that, The base also includes a fixing groove located inside the electrode groove. An adsorption element is provided inside the fixing groove, and the electrode plate is located on the adsorption element. The adsorption element can apply an adsorption force to the electrode plate.

4. The detection device according to claim 3, characterized in that, The material of the adsorption element is silica gel.

5. The detection device according to claim 1, characterized in that, The glass cover is made of the same material as the channel plate.

6. The detection device according to claim 1, characterized in that, The detection device further includes: A gasket is located between the base and the channel plate and is aligned with the second open end of the reaction chamber; at least a portion of the gasket is located on the electrode plate and is sealed to both the second open end and the edge region of the electrode plate.

7. The detection device according to claim 6, characterized in that, The washer is made of rubber.

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