A quantitative sample introduction device and method for capillary electrophoresis

Abstract

This invention discloses a quantitative sample injection device and method for capillary electrophoresis, applied in the field of capillary electrophoresis technology. The key technical points are: it includes a sample vial and a buffer chamber, with a capillary tube fixedly connected between the sample vial and the buffer chamber; a three-way valve is also fixedly provided, with the capillary tube and the buffer chamber respectively fixedly connected to the three-way valve; a quantitative sample injection component for generating negative pressure to achieve sample injection is externally fixedly connected to the three-way valve; a shut-off valve is fixedly connected between the three-way valve and the buffer chamber; and a miniature stopcock valve is fixedly provided between the quantitative sample injection component and the three-way valve. The technical effect is: improving the accuracy of sample injection volume while reducing the cost of the injection device.

Description

Technical Field

[0001] This invention relates to the field of capillary electrophoresis technology, and in particular to a quantitative sample introduction device and method for capillary electrophoresis. Background Technology

[0002] Capillary electrophoresis is a highly efficient separation and analysis technique in analytical chemistry. It uses a capillary with a narrow inner diameter as the separation channel and a high-voltage electric field to drive the electrophoretic separation of sample components. It has significant advantages such as high separation efficiency, fast analysis speed, and small sample volume, and is widely used in many fields such as biomedicine, environmental monitoring, and food safety. As the first key step in the capillary electrophoresis analysis process, the accuracy of sample injection directly determines the accuracy and reliability of subsequent separation and detection results.

[0003] Currently, the mainstream capillary electrophoresis sample introduction methods in the industry mainly include electrodynamic sample introduction and hydrodynamic sample introduction. Electrodynamic sample introduction relies on the synergistic effect of electroosmosis and ion electromigration to introduce the sample into the capillary. Although it is simple to operate, it has obvious electrodiscrimination, which leads to differences in the amount of ions with different charge-to-mass ratios introduced into the sample, ultimately affecting the accuracy of quantitative analysis. Hydrodynamic sample introduction drives the sample through vacuum, pressure, or liquid level difference, but the system structure is complex and the control is difficult. At the same time, since the sample volume required for capillary electrophoresis is extremely small (usually only 4-8 nanoliters), there is a lack of commercially available sample introduction valves that meet this specification, and customized valves are expensive. Therefore, it is necessary to improve the existing capillary electrophoresis sample introduction devices on the market to improve the accuracy of sample volume introduction while reducing the cost of the sample introduction device. Summary of the Invention

[0004] The first objective of this invention is to provide a quantitative sample injection device for capillary electrophoresis, which has the advantage of improving the accuracy of sample injection volume while reducing the cost of the injection device.

[0005] The above-mentioned technical objective of the present invention is achieved through the following technical solution: a quantitative injection device for capillary electrophoresis, comprising a sample vial and a buffer chamber, wherein a capillary tube is fixedly connected between the sample vial and the buffer chamber; a three-way tube is also fixedly provided, wherein the capillary tube and the buffer chamber are respectively fixedly connected to the three-way tube, and a quantitative injection component for generating negative pressure to realize sample injection is externally fixedly connected to the three-way tube; a shut-off valve is fixedly connected between the three-way tube and the buffer chamber, and a miniature stopcock valve is fixedly provided between the quantitative injection component and the three-way tube.

[0006] The present invention is further configured such that: the quantitative injection assembly includes a plunger base fixedly connected to the outlet end of the first micro plug valve; a sealed cavity and a plunger injection cavity are respectively opened in the plunger base; the sealed cavity is connected to the plunger injection cavity; a plunger rod is slidably connected in the plunger injection cavity; a sliding drive assembly is fixedly connected to the plunger base to drive the plunger rod to slide and generate negative pressure in the sealed cavity; and an electromagnetic plug valve for separating the sealed cavity is also fixedly connected to the plunger base in the sealed cavity.

[0007] The present invention is further configured such that: the sliding drive assembly includes a drive screw shaft rotatably connected to one end of the plunger rod away from the sealed cavity, and a drive motor fixedly connected to the plunger base for driving the drive screw shaft to rotate; a drive nut cooperating with the drive screw shaft is fixedly connected inside the plunger base.

[0008] The present invention is further configured such that the drive motor is a stepper motor or a servo motor.

[0009] The present invention is further configured such that: the volume of the plunger injection chamber is between 40 and 80 nanoliters, and the volume ratio of the sealed cavity to the plunger injection chamber is not less than 5:1.

[0010] The present invention is further configured such that: the electromagnetic plug valve divides the sealed cavity into a first cavity connected to the three-way pipe and a second cavity connected to the plunger injection cavity, the bottom surface of the second cavity is higher than the top surface of the first cavity, and the volume ratio of the first cavity to the second cavity is 1:9.

[0011] The present invention is further configured such that: a sample flushing plunger tube communicating with the first cavity is fixedly connected to the plunger base; the sample flushing plunger tube is externally connected to a flushing fluid input tube for inputting flushing fluid and a buffer solution input tube for inputting buffer solution; a sample flushing plunger rod for filling the first cavity with the flushing fluid or buffer solution in the sample flushing plunger tube is slidably connected inside the sample flushing plunger tube; and an electromagnetic switch valve is fixedly connected between the sample flushing plunger tube and the first cavity, and the electromagnetic switch valve is normally closed.

[0012] The present invention is further configured such that: the volume of the sample flushing plunger tube is between 50-100 nanoliters, and the sample flushing plunger tube is provided with a scale to precisely adjust the volume of flushing fluid and buffer solution injected into the first cavity.

[0013] The second objective of this invention is to provide a quantitative sample injection method for capillary electrophoresis, which has the advantage of improving the accuracy of sample injection volume while reducing the cost of the injection device.

[0014] The above-mentioned technical objective of the present invention is achieved through the following technical solution: a quantitative sample introduction method for capillary electrophoresis, using a quantitative sample introduction device for capillary electrophoresis as described in the above technical solution; comprising: Step 1: Fix the inlet end of the capillary tube below the liquid surface of the sample vial to ensure that the capillary tube is completely immersed. Insert and fix the outlet end of the capillary tube into one end of the three-way tube. Connect the other two ends of the three-way tube to the buffer chamber and the quantitative injection assembly, respectively. Step 2: Close the shut-off valve and the micro plug valve, open the electromagnetic plug valve, and the sliding drive assembly drives the plunger rod to slide outward a certain distance to reduce the air pressure in the sealed cavity and the plunger injection chamber; Step 3: Replenish buffer solution into the sample flushing plunger tube by pulling the plunger rod outward. Then open the solenoid valve to fill the first chamber with the replenishing solution in the sample flushing plunger tube until the volume of the first chamber is the same as the required sample volume. Then close the solenoid valve and the solenoid stopcock valve. Step 4: Open the micro stopcock valve. Since the air pressure in the first chamber is lower than the air pressure in the sample vial, the sample in the sample vial is drawn into the capillary. At the same time, the remaining buffer solution in the capillary enters the first chamber until it is completely filled. The volume of the sample drawn into the capillary is equal to the volume of the buffer solution entering the first chamber. Open the shut-off valve. Step 5: Insert the capillary sample inlet end into the buffer chamber, and energize the high-voltage electrode to apply a high-voltage electric field to both ends of the capillary. The sample components in the capillary are separated by electrophoresis under the drive of the high-voltage electric field, and the separated sample components are detected by the detector. Step 6: Rinse and maintain the capillary tube.

[0015] The present invention is further configured such that step 6 includes: Step 6.1: Close the solenoid valve, fill the flushing fluid into the sample flushing plunger tube through the flushing fluid inlet tube, and at the same time insert the sample inlet end of the capillary tube into the flushing chamber. Step 6.2: Open the electromagnetic switch valve and close the shut-off valve and electromagnetic plug valve. Push the sample flushing plunger rod inward. The flushing liquid in the sample flushing plunger tube passes through the first chamber, the three-way tube and the capillary tube in sequence and finally flows into the flushing chamber to complete the flushing. Step 6.3: Repeat steps 1-5 above.

[0016] In summary, the present invention has the following beneficial effects: 1. By setting up a three-way connector to connect the capillary tube, buffer chamber, and quantitative injection component, and installing a shut-off valve between the three-way connector and the buffer chamber, and a miniature stopcock valve between the quantitative injection component and the three-way connector, a sliding drive assembly moves the plunger rod to generate negative pressure in the sealed cavity, thereby achieving negative pressure aspiration of the sample. This avoids the problem of uneven sample volume due to charge-to-mass ratio caused by electrodiscrimination in existing electric injection methods. Simultaneously, the quantitative injection component uses an electromagnetic stopcock valve to divide the sealed cavity into a first chamber and a second chamber, with the volume ratio of the first chamber to the second chamber set at 1:9. The volume of the plunger injection chamber is set between 40-80 nanoliters. The volume ratio of the sealed cavity to the plunger injection chamber is no less than 5:1, which achieves precise control of the negative pressure intensity and the injection volume. At the same time, by setting a sample flushing plunger tube connected to the first chamber on the plunger base, when negative pressure is generated in the sealed cavity, buffer solution is precisely added to the first chamber to ensure that the remaining volume in the first chamber is the same as the required sample volume while maintaining negative pressure in the first chamber. This makes the sample volume drawn into the capillary equal to the buffer solution volume entering the first chamber. By controlling the precision of adding a large volume of buffer solution to the first chamber, the accuracy of quantitative sample injection is indirectly improved. The structure is simple and reduces the cost of quantitative sample injection. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of this embodiment; Figure 2 This is a schematic diagram of the quantitative injection component in this embodiment.

[0018] Reference numerals: 1. Sample vial; 2. Buffer chamber; 3. Capillary tube; 4. Three-way valve; 5. Quantitative injection assembly; 51. Plunger base; 52. Sealed cavity; 53. Plunger injection chamber; 54. Plunger rod; 55. Sliding drive assembly; 551. Drive screw shaft; 552. Drive motor; 553. Drive nut; 56. Electromagnetic stopcock valve; 57. First chamber; 58. Second chamber; 59. Sample flushing plunger tube; 510. Flushing solution inlet tube; 511. Buffer inlet tube; 512. Sample flushing plunger rod; 513. Electromagnetic switch valve; 6. Shut-off valve; 7. Miniature stopcock valve; 8. Flushing chamber. Detailed Implementation

[0019] The present invention will be further described in detail below with reference to the accompanying drawings.

[0020] Example 1: refer to Figure 1A quantitative injection device for capillary electrophoresis includes a sample vial 1 and a buffer chamber 2. A capillary tube 3 is fixedly connected between the sample vial and the buffer chamber 2. A three-way tube 4 is also fixedly provided. The capillary tube 3 and the buffer chamber 2 are respectively fixedly connected to the three-way tube 4. A quantitative injection component 5 for generating negative pressure to realize sample injection is also externally fixedly connected to the three-way tube 4. A shut-off valve 6 is fixedly connected between the three-way tube 4 and the buffer chamber 2. A miniature stopcock valve 7 is fixedly provided between the quantitative injection component 5 and the three-way tube 4.

[0021] refer to Figure 1 and Figure 2 Specifically, the quantitative injection assembly 5 includes a plunger base 51 fixedly connected to the outlet end of the first micro plug valve 7. A sealed cavity 52 and a plunger injection chamber 53 are respectively formed within the plunger base 51. The sealed cavity 52 and the plunger injection chamber 53 are connected. A plunger rod 54 is slidably connected within the plunger injection chamber 53. A sliding drive assembly 55 is fixedly connected to the plunger base 51 to drive the plunger rod 54 to slide and generate negative pressure within the sealed cavity 52. An electromagnetic stopcock valve 56 is also fixedly connected within the sealed cavity 52 to separate the sealed cavity 52. ​​The electromagnetic stopcock valve 56 divides the sealed cavity 52 into a first cavity 57 connected to the three-way tube 4 and a second cavity 58 connected to the plunger injection chamber 53. The plunger rod 54 is driven to slide by the sliding drive assembly 55, thereby generating negative pressure in the sealed cavity 52 and the plunger injection chamber 53. The bottom surface of the second cavity 58 is higher than the top surface of the first cavity 57 to avoid buffer solution leakage. Entering the second chamber 58, the volume ratio of the first chamber 57 to the second chamber 58 is 1:9. The volume of the plunger injection chamber 53 is between 40-80 nanoliters, and the volume ratio of the sealed cavity 52 to the plunger injection chamber 53 is not less than 5:1, meaning the volume of the sealed cavity 52 is at least 200-400 nanoliters. Subsequently, the volume of the first chamber 57 is between 20-40 nanoliters. When the sliding drive assembly 55 drives the plunger rod 54 to slide outwards, the sealed cavity 52 and... The increased volume of the plunger injection chamber 53 leads to a decrease in gas pressure, while the gas pressure in the sample vial 1 remains unchanged. When the micro stopcock valve 7 is opened, the sample is drawn into the capillary tube 3, and at the same time, the initial buffer solution in the capillary tube 3 enters the sealed cavity 52. ​​By setting the volume ratio of the sealed cavity 52 to the plunger injection chamber 53 to be no less than 5:1, the negative pressure intensity in the sealed cavity 52 can be precisely adjusted by adjusting the sliding distance of the plunger rod 54, thereby achieving precise adjustment of the injection volume.

[0022] refer to Figure 1Specifically, the sliding drive assembly 55 includes a drive screw shaft 551 rotatably connected to the end of the plunger rod 54 away from the sealed cavity 52, and a drive motor 552 slidably connected to the plunger base 51 for rotating the drive screw shaft 551. The drive motor 552 is fixed on a sliding seat, which is slidably connected to the plunger base 51 based on a sliding guide rail. A drive nut 553 that cooperates with the drive screw shaft 551 is fixedly connected inside the plunger base 51. The drive motor 552 drives the drive screw shaft 551 to rotate, thereby driving the plunger rod 54 to slide along the direction of the plunger injection chamber 53 to generate negative pressure in the sealed cavity 52 and the plunger injection chamber 53. In this embodiment, the drive motor 552 is a stepper motor or a servo motor to improve the sliding accuracy of the plunger rod 54.

[0023] refer to Figure 2 Specifically, a sample flushing plunger tube 59, communicating with the first cavity 57, is fixedly connected to the plunger base 51. A flushing fluid inlet tube 510 and a buffer solution inlet tube 511 are externally connected to the sample flushing plunger tube 59. Both the flushing fluid inlet tube 510 and the buffer solution inlet tube 511 are equipped with switching valves to control the on / off state. A sample flushing plunger rod 512 is slidably connected inside the sample flushing plunger tube 59 to fill the first cavity 57 with the flushing fluid or buffer solution from the sample flushing plunger tube. An electromagnetic switch valve 513 is fixedly connected between the sample flushing plunger tube 59 and the first cavity 57. The electromagnetic switch valve 513 is normally closed. The volume of the sample flushing plunger tube 59 is between 50 and 100 nanoliters. The sample flushing plunger tube 59 is marked with graduations to precisely adjust the volume of flushing fluid and buffer solution injected into the first cavity 57. When injection is performed, the sealed cavity is first precisely lowered by the sliding drive assembly 55. The pressure in the plunger injection chamber 53 is adjusted according to the pressure of the plunger. Then, the replenishing solution in the plunger tube 59 is filled into the first chamber 57 until the volume of the first chamber 57 is equal to the required sample volume. Finally, the micro stopcock valve 7 is opened. Because the pressure in the first chamber 57 is lower than the pressure in the sample vial 1, the sample in the sample vial 1 is drawn into the capillary tube 3. At the same time, the remaining buffer solution in the capillary tube 3 enters the first chamber 57 until it is completely filled. The volume of sample drawn into the capillary tube 3 is equal to the volume of buffer solution entering the first chamber 57, thus accurately ensuring that the sample injection volume is maintained between 4-8 nanoliters each time. However, after the injection is completed, there will always be a small amount of air in the first chamber 57. To reduce errors and ensure that the air volume in the first chamber 57 is as small as possible, the sample vial 1 is pressurized before opening the micro stopcock valve 7 to increase the pressure difference between the sample vial 1 and the first chamber 57, thereby reducing the error in the injection volume and ensuring the accuracy of the injection volume.

[0024] Example 2: A quantitative sample introduction method for capillary electrophoresis, using a quantitative sample introduction device for capillary electrophoresis as described in claim 7, comprising: Step 1: Fix the inlet end of the capillary tube 3 below the liquid surface of the sample bottle 1 to ensure that the capillary tube 3 is completely immersed. The outlet end of the capillary tube 3 is inserted and fixed to one end of the three-way tube 4. The other two ends of the three-way tube 4 are connected to the buffer chamber 2 and the quantitative injection component 5, respectively. Step 2: Close the shut-off valve 6 and the miniature plug valve 7, open the electromagnetic plug valve 56, and the sliding drive assembly 55 drives the plunger rod 54 to slide outward a certain distance to reduce the air pressure in the sealed cavity 52 and the plunger injection cavity 53. Step 3: By pulling the sample flushing plunger rod 512 outward, buffer solution is added to the sample flushing plunger tube 59. Then, the solenoid switch valve 513 is opened to fill the first chamber 57 with the replenishing solution in the sample flushing plunger tube 59 until the volume of the first chamber 57 is the same as the required sample volume. Then, the solenoid switch valve 513 and the solenoid stopcock valve 56 are closed. Step 4: Open the micro stopcock valve 7. Since the air pressure in the first chamber 57 is lower than the air pressure in the sample bottle 1, the sample in the sample bottle 1 is drawn into the capillary tube 3. At the same time, the remaining buffer solution in the capillary tube 3 enters the first chamber 57 until it is completely filled. The volume of the sample drawn into the capillary tube 3 is equal to the volume of the buffer solution entering the first chamber 57. Open the shut-off valve 6. Step 5: Insert the sample inlet end of capillary 3 into buffer chamber 2, and energize the high voltage electrode to apply a high voltage electric field to both ends of capillary 3. The sample components in capillary 3 are separated by electrophoresis under the drive of the high voltage electric field, and the separated sample components are detected by the detector. Step 6: Rinse and maintain capillary tube 3; Step 6.1: Close the solenoid switch valve 513, and fill the flushing fluid into the sample flushing plunger tube 59 through the flushing fluid inlet tube 510. At the same time, insert the sample inlet end of the capillary tube 3 into the flushing chamber 8. Step 6.2: Open the solenoid switch valve 513 and close the shut-off valve 6 and the solenoid plug valve 56. Push the sample flushing plunger rod 512 inward. The flushing liquid in the sample flushing plunger tube 59 passes through the first chamber 57, the three-way tube 4 and the capillary tube 3 in sequence and finally flows into the flushing chamber 8 to complete the flushing. Step 6.3: Repeat steps 1-5 above.

[0025] This specific embodiment is merely an explanation of the present invention and is not intended to limit the invention. After reading this specification, those skilled in the art can make inventive modifications to this embodiment as needed, but as long as they are within the scope of the claims of the present invention, they are protected by patent law.

Claims

1. A quantitative sample introduction device for capillary electrophoresis, comprising a sample vial (1) and a buffer chamber (2), wherein a capillary tube (3) is fixedly connected between the sample vial and the buffer chamber (2); characterized in that, A three-way tube (4) is also fixedly provided. The capillary tube (3) and the buffer chamber (2) are respectively fixedly connected to the three-way tube (4). A quantitative injection component (5) for generating negative pressure to realize sample injection is also fixedly connected to the three-way tube (4). A shut-off valve (6) is fixedly connected between the three-way tube (4) and the buffer chamber (2). A miniature stopcock valve (7) is fixedly provided between the quantitative injection component (5) and the three-way tube (4).

2. The quantitative sample introduction device for capillary electrophoresis according to claim 1, characterized in that, The quantitative injection assembly (5) includes a plunger base (51) fixedly connected to the outlet end of the first micro plug valve (7). The plunger base (51) is provided with a sealed cavity (52) and a plunger injection cavity (53). The sealed cavity (52) and the plunger injection cavity (53) are connected. A plunger rod (54) is slidably connected in the plunger injection cavity (53). A sliding drive assembly (55) is fixedly connected to the plunger base (51) to drive the plunger rod (54) to slide and generate negative pressure in the sealed cavity (52). An electromagnetic plug valve (56) for separating the sealed cavity (52) is also fixedly connected to the plunger base (51) in the sealed cavity (52).

3. The quantitative sample introduction device for capillary electrophoresis according to claim 2, characterized in that, The sliding drive assembly (55) includes a drive screw shaft (551) rotatably connected to one end of the plunger rod (54) away from the sealed cavity (52) and a drive motor (552) slidably connected to the plunger base (51) for driving the drive screw shaft (551) to rotate. A drive nut (553) that cooperates with the drive screw shaft (551) is fixedly connected inside the plunger base (51).

4. The quantitative sample introduction device for capillary electrophoresis according to claim 3, characterized in that, The drive motor (552) is a stepper motor or a servo motor.

5. A quantitative sample introduction device for capillary electrophoresis according to claim 2, characterized in that, The volume of the plunger injection chamber (53) is between 40 and 80 nanoliters, and the volume ratio of the sealed cavity (52) to the plunger injection chamber (53) is not less than 5:

1.

6. The quantitative sample introduction device for capillary electrophoresis according to claim 5, characterized in that, The electromagnetic plug valve (56) divides the sealed cavity (52) into a first cavity (57) connected to the three-way pipe (4) and a second cavity (58) connected to the plunger injection cavity (53). The bottom surface of the second cavity (58) is higher than the top surface of the first cavity (57). The volume ratio of the first cavity (57) to the second cavity (58) is 1:

9.

7. A quantitative sample introduction device for capillary electrophoresis according to claim 6, characterized in that, A sample flushing plunger tube (59) communicating with the first cavity (57) is also fixedly connected to the plunger base (51). The sample flushing plunger tube (59) is externally connected to a flushing fluid input tube (510) for inputting flushing fluid and a buffer solution input tube (511) for inputting buffer solution. A sample flushing plunger rod (512) for filling the flushing tube or buffer solution in the sample flushing plunger tube (59) into the first cavity (57) is slidably connected inside the sample flushing plunger tube (59). An electromagnetic switch valve (513) is fixedly connected between the sample flushing plunger tube (59) and the first cavity (57). The electromagnetic switch valve (513) is normally closed.

8. A quantitative sample introduction device for capillary electrophoresis according to claim 7, characterized in that, The volume of the sample flushing plunger tube (59) is between 50 and 100 nanoliters. The sample flushing plunger tube (59) is provided with a scale to precisely adjust the volume of flushing fluid and buffer solution injected into the first cavity (57).

9. A quantitative sample introduction method for capillary electrophoresis, using the quantitative sample introduction device for capillary electrophoresis as described in claim 7; characterized in that, include: Step 1: Fix the inlet end of the capillary tube (3) below the liquid surface of the sample bottle (1) to ensure that the capillary tube (3) is completely immersed. The outlet end of the capillary tube (3) is inserted and fixed on one end of the three-way tube (4). The other two ends of the three-way tube (4) are connected to the buffer chamber (2) and the quantitative injection component (5) respectively. Step 2: Close the shut-off valve (6) and the micro plug valve (7), open the electromagnetic plug valve (56), and the sliding drive assembly (55) drives the plunger rod (54) to slide outward a certain distance to reduce the air pressure in the sealed cavity (52) and the plunger injection chamber (53); Step 3: By pulling the sample flushing plunger rod (512) outward, buffer solution is added to the sample flushing plunger tube (59). Then, the solenoid switch valve (513) is opened to fill the first chamber (57) with the replenishing solution in the sample flushing plunger tube (59) until the volume of the first chamber (57) is the same as the required sample volume. Then, the solenoid switch valve (513) and the solenoid stopcock valve (56) are closed. Step 4: Open the micro stopcock valve (7). Since the air pressure in the first chamber (57) is lower than the air pressure in the sample bottle (1), the sample in the sample bottle (1) is drawn into the capillary tube (3). At the same time, the remaining buffer solution in the capillary tube (3) enters the first chamber (57) until it is completely filled. The volume of the sample drawn into the capillary tube (3) is equal to the volume of the buffer solution entering the first chamber (57). Open the shut-off valve (6). Step 5: Insert the sample inlet end of the capillary (3) into the buffer chamber (2), and energize the high voltage electrode to apply a high voltage electric field to both ends of the capillary (3). The sample components in the capillary (3) are separated by electrophoresis under the drive of the high voltage electric field, and the separated sample components are detected by the detector. Step 6: Rinse and maintain the capillary tube (3).

10. A quantitative sample introduction method for capillary electrophoresis according to claim 9, characterized in that, Step 6 includes: Step 6.1: Close the electromagnetic switch valve (513), and fill the flushing fluid into the sample flushing plunger tube (59) through the flushing fluid input tube (510). At the same time, insert the sample inlet end of the capillary tube (3) into the flushing chamber (8). Step 6.2: Open the electromagnetic switch valve (513) and close the shut-off valve (6) and electromagnetic plug valve (56). Push the sample flushing plunger rod (512) inward. The flushing liquid in the sample flushing plunger tube (59) passes through the first chamber (57), the three-way tube (4) and the capillary tube (3) in sequence and is finally discharged into the flushing chamber (8) to complete the flushing. Step 6.3: Repeat steps 1-5 above.

Images