Preservative liquid for prolonging life of cut flowers of ixora and preparation method thereof

Abstract

The application discloses a preservative solution for prolonging the life of cut flowers of Impatiens walleriana and a preparation method thereof, and relates to the field of postharvest preservation technology of Impatiens walleriana. The preservative solution is composed of glucose, gibberellin, 8-hydroxyquinoline sulfate, citric acid and deionized water in a specific proportion, wherein the glucose is 3.0% (w / v), the gibberellin is 25 mg / L, the 8-hydroxyquinoline sulfate is 100 mg / L, the citric acid is 80 mg / L, and the balance is deionized water. When the preservative solution is prepared, the gibberellin is first dissolved in anhydrous ethanol to prepare an alcohol-soluble mother liquor, the 8-hydroxyquinoline sulfate is dissolved in deionized water to prepare an aqueous mother liquor, and then the glucose and the citric acid solution are mixed step by step and constant volume is performed, so that a uniform transparent preservative solution is finally prepared. According to the scheme, the cell membrane integrity of the cut flowers of Impatiens walleriana can be effectively maintained, petal browning and water loss can be delayed, the best viewing period and the vase life of the cut flowers can be significantly prolonged, the commodity value can be improved, raw materials are conventional, the process is simple, and the scheme is suitable for large-scale application.

Description

Technical Field

[0001] This invention relates to the field of post-harvest preservation technology for Ixora flowers, specifically providing a preservative solution for extending the life of cut Ixora flowers and its preparation method. Background Technology

[0002] Ixora, an evergreen shrub or small tree belonging to the Rubiaceae family, is an excellent ornamental plant. It boasts a long flowering period, from March to December, and can bloom year-round in suitable temperatures. With rich and vibrant flower colors and a full, graceful form, it is drought-tolerant, thrives in high temperatures, and is widely adaptable. Ixora has high application value in the flower market, performing exceptionally well not only in landscape design but also in the cut flower sector. Its upright branches and compact, full inflorescences make it suitable for bouquets, flower baskets, and various celebrations and home decorations. It serves as a high-quality floral expression of emotion, and market demand continues to rise.

[0003] Post-harvest, Ixora cut flowers are susceptible to triple stress: water imbalance, nutrient depletion, and ethylene sensitivity, leading to problems such as petal browning, loose inflorescences, and bent stems. This severely restricts the large-scale expansion of its high-end cut flower market. Current technologies lack a good match between general-purpose cut flower preservatives and the specific physiological metabolic pathways of Ixora, failing to simultaneously address the three core challenges of water retention, color preservation, and shape maintenance. The lack of a dedicated preservative developed specifically for the post-harvest physiological characteristics of Ixora has become a key technological bottleneck for the commercial promotion of Ixora cut flowers. Summary of the Invention

[0004] To address the aforementioned problems, the present invention proposes the following technical solution: a preservative solution for extending the lifespan of cut Ixora flowers, comprising:

[0005] Glucose: 3.0% (w / w), 3% (w / v) by mass and volume.

[0006] Gibberellin (GA3): 0.0025% (w / w), mass / volume concentration 25 mg / L;

[0007] 8-Hydroxyquinoline sulfate (8-HQS): 0.01% (w / w), mass-volume concentration 100 mg / L;

[0008] Citric acid (CA): 0.008% (w / w), mass-volume concentration 80 mg / L;

[0009] Deionized water: balance, with a ratio of 96.9795% (w / w), used as a solvent to make up to the total mass of the preservative solution.

[0010] Preferably, the preparation method of the gibberellin (GA3) includes the following steps: take a certain amount of GA3 powder, add 5 times its mass of anhydrous ethanol, stir magnetically at room temperature until completely dissolved, and obtain GA3 alcohol solution for later use.

[0011] Preferably, the preparation method of the 8-hydroxyquinoline sulfate (8-HQS) includes the following steps: take a certain amount of 8-HQS powder, add 20 times its mass of deionized water, heat to 40°C and stir magnetically until completely dissolved, and cool to room temperature for later use.

[0012] A method for preparing a preservative solution to extend the shelf life of cut Ixora flowers includes the following steps:

[0013] S1. Weigh out glucose, add deionized water, and stir at 300 r / min for 5 min until completely dissolved;

[0014] S2. Add citric acid (CA) powder and stir at 300 rpm for 3 minutes until completely dissolved;

[0015] S3. Add the aqueous mother liquor containing 8-HQS and stir at 300r / min for 2min until the mixture is homogeneous.

[0016] S4. Add the alcohol-soluble mother liquor of GA3 and stir at 300 r / min for 5 min until the mixture is homogeneous;

[0017] S5. Transfer to a 1000mL volumetric flask, dilute to the mark with deionized water, and shake well by inverting 3-5 times to obtain a homogeneous and transparent preservative solution.

[0018] Preferably, in step S1, a magnetic stirrer is used to stir the glucose with added deionized water.

[0019] Preferably, the transparent preservative solution prepared in steps S1-S5 is quickly transferred to a light-proof, sealed storage bottle using a pipette and stored at room temperature.

[0020] Preferably, the entire preparation process of the preservative liquid is carried out at room temperature (25±2℃) and standard atmospheric pressure (101.325kPa).

[0021] The beneficial effects of this invention are:

[0022] (1) Significantly extended vase life and optimal viewing period. After treatment with the vase solution of the present invention, the optimal viewing period of cut Ixora flowers was extended from 6.33±0.94d in the control group to 9.63±0.58d, an extension of 3.3d; the vase life was extended from 9.33±0.47d in the control group to 13±0.82d, an extension of 3.67d, with a significant preservation effect, greatly improving the commercial circulation cycle of cut Ixora flowers.

[0023] (2) Effectively maintains cell membrane integrity and delays cut flower senescence. The malondialdehyde (MDA) content and relative conductivity of cut flowers in the vase solution treatment group were significantly lower than those in the water control group, indicating that the preservation solution of the present invention can significantly reduce the degree of damage to the cell membrane of Ixora cut flowers, enhance cell structure stability, delay the senescence process of cut flowers at the cellular level, and solve the core problem of post-harvest flower branch dehydration and wilting.

[0024] (3) Significantly improves the ornamental quality of cut flowers and enhances their commercial value. The anthocyanin and chlorophyll content of cut flowers in the vase solution treatment group was significantly higher than that in the water control group. It can effectively maintain the bright color of the flowers and the green color of the leaves, making the flowers more plump and the inflorescence more compact. From the perspective of color preservation and shape preservation, it improves the ornamental quality of Ixora cut flowers and significantly increases their commercial value.

[0025] (4) The formula and process are highly compatible and have broad prospects for commercial application. The reagents used in the vase solution formula of this invention are all conventional flower preservation raw materials, which are easy to obtain and low in cost. The compounding ratio is accurate and the process is simple to operate. No complicated equipment is required. The harvesting and pretreatment process is in line with the actual production scenario of flower planting bases, which is convenient for large-scale promotion and application. It provides scientific and feasible technical support for the commercial production of Ixora cut flowers, and also provides technical reference for the sustainable development of the flower industry. Attached Figure Description

[0026] Figure 1 This diagram illustrates the effect of the preservative solution treatment of the present invention on the state of cut Ixora flowers.

[0027] Figure 2 This diagram illustrates the effect of the preservative solution of this invention on the permeability of the petal membrane of cut Ixora flowers.

[0028] Figure 3 This is a graph showing the effect of the preservative solution of the present invention on the anthocyanin and chlorophyll content of cut Ixora flowers.

[0029] Figure 4 This is a flowchart illustrating the preparation process of the preservative solution of this invention. Detailed Implementation

[0030] The present invention will be further described below with reference to embodiments. Example 1

[0031] In response to the unique physiological characteristics of Ixora cut flowers, such as postharvest water imbalance, nutrient depletion, and ethylene sensitivity, a special bottle-preserving solution compound formula suitable for their physiological metabolism was screened and optimized through single-factor experiments combined with orthogonal experiments.

[0032] The Influence of Single-Factor Chemical Composition of Preservative Solution on the Vase Life of Cut Flowers

[0033]

[0034] Note: The data in the table are mean ± standard deviation. Different lowercase letters indicate the significance level of P < 0.05.

[0035] Orthogonal experimental results

[0036]

[0037] Note: The data in the table are mean ± standard deviation. Different lowercase letters indicate the significance level of P < 0.05.

[0038] The above tables show the effects of single-factor chemical composition of the preservative solution on the vase life of cut flowers, as well as the results of orthogonal experiments. They clearly demonstrate the single-factor experimental data for different concentrations of each reagent and the optimization process and results of the orthogonal experiment, providing data support for the scientific validity of the vase solution formula of this invention. The precise mass percentages of each component are clearly defined: 3.0% glucose, 0.0025% GA. 3、 0.01% 8-HQS and 0.008% CA simultaneously address water retention, color preservation, and shape preservation. The optimal practical concentrations for each preservative were determined: 25 mg / L GA3, 100 mg / L 8-HQS, 80 mg / L CA, and 3% (w / v) glucose. These reagents work synergistically: glucose provides continuous nutrient supply; GA3 delays pigment degradation and flower senescence; 8-HQS provides antibacterial and preservative effects while retaining water; and CA adjusts the solution's pH and stabilizes cell membranes, maximizing the preservation effect. Furthermore, addressing the solubility limitations of GA3 and 8-HQS, a targeted pretreatment process was developed to resolve the dissolution of poorly soluble components. Combined with a room temperature and pressure preparation environment, a standardized stirring speed of 300 rpm, and a step-by-step mixing sequence, this ensures complete dissolution, homogeneity, and stability of all components in the preservative solution, achieving 100% utilization of the effective ingredients.

[0039] The following are the specific steps for preparing a 1L bottle-preserving solution:

[0040] S1. Weigh 30.0g of glucose (precisely matching the 3% ratio), add 500mL of deionized water, and stir magnetically at 300r / min for 5min until completely dissolved;

[0041] S2. Add 80.0 mg of CA powder and stir at 300 r / min for 3 min until completely dissolved;

[0042] S3. Add the water-soluble mother liquor containing 100.0 mg 8-HQS, and stir at 300 r / min for 2 min until the mixture is homogeneous;

[0043] S4. Add the alcohol-soluble mother liquor containing 25.0 mg GA3, and stir at 300 r / min for 5 min until the mixture is homogeneous;

[0044] S5. Transfer to a 1000mL volumetric flask, dilute to the mark with deionized water, and shake well by inverting 3-5 times to obtain a homogeneous and transparent preservation solution.

[0045] Finally, the prepared preservation solution is quickly transferred to a light-proof, sealed storage bottle using a pipette and stored at room temperature. Example 2

[0046] Fresh-cut flowers were harvested at 8:00 AM, selecting healthy, disease-free flower stems with a diameter of approximately 0.4 cm, a length of 30 cm, and an inflorescence diameter of 6 cm, bearing 8-12 open florets. The first and second pairs of leaves below the inflorescence were retained, while the remaining leaves were removed. The prepared flower stems were placed in pure water, and the base of the stem was trimmed 3 cm at an angle. Before vase arrangement, the base of the stem was cut again at a 45° angle in the water to remove air pockets and prevent water absorption obstruction. The appearance of the cut Ixora flowers at different vase times was compared between a water control group and the group treated with the preservative solution of this invention.

[0047] Reference Appendix Figure 1 The figure shows a comparison of the appearance of cut Ixora flowers between the water control group and the preservative solution treatment group at different vase times. It visually demonstrates that the cut flowers in the treatment group are significantly better than those in the control group in terms of flower fullness, color vibrancy, leaf greenness, and stem uprightness, and the aging process is significantly delayed. Example 3

[0048] Reference Appendix Figure 2 The figure contains two subplots, A and B. Plot A shows the change in malondialdehyde (MDA) content with the time of immersion in a bottle, and plot B shows the change in relative conductivity with the time of immersion in a bottle. The horizontal axis represents the immersion time (d), and the vertical axis represents the MDA content and relative conductivity, respectively. The figure clearly shows that the MDA content and relative conductivity of the treatment group are significantly lower than those of the control group at each immersion time point, indicating that the treatment group has less cell membrane damage and better cell membrane integrity.

[0049] Reference Appendix Figure 3 The figure contains two subplots, A and B. A is the curve showing the change in anthocyanin content with vase time, and B is the curve showing the change in chlorophyll content with vase time. The horizontal axis represents vase time (d), and the vertical axes represent anthocyanin content and chlorophyll content, respectively. The figure shows that the anthocyanin and chlorophyll contents of the treatment group are significantly higher than those of the control group at each vase time point, indicating that the treatment group can effectively maintain the pigment content of cut flowers and maintain good ornamental quality.

[0050] The present invention and its embodiments have been described above. This description is not restrictive, and the accompanying drawings are only one embodiment of the present invention; the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the spirit of the invention, such designs should fall within the protection scope of the present invention.

Claims

1. A preservative solution for extending the shelf life of cut Ixora flowers, characterized in that, Include: Glucose: 3.0% (w / w), 3% (w / v) by mass and volume. Gibberellin (GA3): 0.0025% (w / w), mass / volume concentration 25 mg / L; 8-Hydroxyquinoline sulfate (8-HQS): 0.01% (w / w), mass / volume concentration 100 mg / L; Citric acid (CA): 0.008% (w / w), mass-volume concentration 80 mg / L; Deionized water: balance, with a ratio of 96.9795% (w / w), used as a solvent to make up to the total mass of the preservative solution.

2. The preservative solution for extending the shelf life of cut Ixora flowers according to claim 1, characterized in that: The preparation method of the gibberellin (GA3) includes the following steps: take a certain amount of GA3 powder, add 5 times its mass of anhydrous ethanol, stir magnetically at room temperature until completely dissolved, and obtain GA3 alcohol solution mother liquor for later use.

3. The preservative solution for extending the shelf life of cut Ixora flowers according to claim 1, characterized in that: The preparation method of the 8-hydroxyquinoline sulfate (8-HQS) includes the following steps: take a certain amount of 8-HQS powder, add 20 times its mass of deionized water, heat to 40°C and stir magnetically until completely dissolved, and cool to room temperature for later use.

4. A method for preparing a preservative solution to extend the shelf life of cut Ixora flowers, characterized in that: Includes the following steps: S1. Weigh out glucose, add deionized water, and stir at 300 r / min for 5 min until completely dissolved; S2. Add citric acid (CA) powder and stir at 300 rpm for 3 minutes until completely dissolved; S3. Add the aqueous mother liquor containing 8-HQS and stir at 300r / min for 2min until the mixture is homogeneous. S4. Add the alcohol-soluble mother liquor of GA3 and stir at 300 r / min for 5 min until the mixture is homogeneous; S5. Transfer to a 1000mL volumetric flask, dilute to the mark with deionized water, and shake well by inverting 3-5 times to obtain a homogeneous and transparent preservative solution.

5. The method for preparing a preservative solution for extending the shelf life of cut Ixora flowers according to claim 4, characterized in that: In step S1, a magnetic stirrer is used to stir the glucose with added deionized water.

6. The method for preparing a preservative solution for extending the shelf life of cut Ixora flowers according to claim 4, characterized in that: The transparent preservative solution prepared in steps S1-S5 is quickly transferred to a light-proof, sealed storage bottle using a pipette and stored at room temperature.

7. The method for preparing a preservative solution for extending the shelf life of cut Ixora flowers according to claim 4, characterized in that: The entire preparation process of the preservative solution was carried out at room temperature (25±2℃) and standard atmospheric pressure (101.325kPa).

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