A multi-effect anti-aging composition, anti-wrinkle cosmetic and method of making the same

Abstract

The application relates to the technical field of cosmetics, and particularly discloses a multi-effect anti-aging composition, an anti-wrinkle cosmetic and a preparation method thereof. The multi-effect anti-aging composition comprises the following components in parts by weight: 0.05-0.5 parts of acetylated sodium hyaluronate; 0.1-0.2 parts of hydrolyzed soybean fine powder; 0.1-0.2 parts of tetrahydro methyl pyrimidine carboxylic acid; and 0.1-0.2 parts of fibronectin; the hydrolyzed soybean fine powder contains a plurality of oligopeptides with a molecular weight lower than 180 daltons. The multi-effect anti-aging composition can be used in cosmetics and has the advantages of excellent multi-effect anti-aging and anti-wrinkle performance.

Description

Technical Field

[0001] This application relates to the technical field of cosmetics, and more specifically, to a multi-effect anti-aging composition, an anti-wrinkle cosmetic, and a method for preparing the same. Background Technology

[0002] Aging is a complex biological process involving endogenous aging (natural aging) and exogenous aging (primarily photoaging). Its mechanisms include the degradation of extracellular matrix (ECM) components such as collagen and elastin, oxidative stress, inflammatory responses, and decreased cell renewal capacity. Among these, the overexpression of matrix metalloproteinases (MMPs) is a key factor leading to ECM degradation and wrinkle formation. Furthermore, inflammatory cytokines such as TNF-α, IL-1, and IL-6, produced by cells due to inflammation, can induce MMP production, leading to skin wrinkles and sagging.

[0003] Currently, anti-wrinkle skincare products on the market come in various forms, such as masks, toners, creams, and serums. Among them, serums are widely used due to their high concentration of active ingredients and good penetration. Current serums often employ a combination of multiple peptides. This approach represents the mainstream technological thinking at present, which is to enhance the effect by layering peptides with different functions. However, this approach is still limited to the "signal peptide" level, and its effects on timely repair of the skin barrier, deep nutrient supply, and overall regulation of the cellular microenvironment are limited, resulting in significant room for improvement in anti-wrinkle efficacy. Summary of the Invention

[0004] To address the issues of insufficient synergistic effects of anti-wrinkle ingredients in current anti-aging compositions and limited ability to repair the skin barrier and regulate the inflammatory microenvironment, this application provides a multi-effect anti-aging composition, an anti-wrinkle cosmetic, and a method for preparing the same.

[0005] In a first aspect, this application provides a multi-effect anti-aging composition, which adopts the following technical solution: A multi-effect anti-aging composition comprising the following components in parts by weight: 0.05-0.5 parts of acetylated sodium hyaluronate; 0.1-0.2 parts of hydrolyzed soybean powder; 0.1-0.2 parts of tetrahydromethylpyrimidine carboxylic acid; Fibronectin 0.1-0.2 parts; The hydrolyzed soybean powder contains a variety of oligopeptides with a molecular weight of less than 180 Daltons.

[0006] By employing the above-mentioned technical solution, acetylated sodium hyaluronate is obtained from the natural moisturizing factor sodium hyaluronate through an acetylation reaction. The introduction of the acetyl group gives acetylated sodium hyaluronate both hydrophilic and lipophilic properties, increasing the product's bioavailability by 6 times. It can exert bioactive functions such as double moisturizing, repairing the stratum corneum barrier, and improving skin elasticity, thereby improving dry and rough skin, leaving it soft and supple.

[0007] Hydrolyzed soybean powder is a highly effective complex polypeptide product made from over 7,700 oligopeptides extracted from soybeans. It can instantly repair and firm the skin. The oligopeptides are all short peptides with a molecular weight of less than 180 Daltons, allowing them to directly reach the basal layer of the skin to provide nutrition, resulting in significant, safe, and immediate effects.

[0008] Fibronectin can bind to T lymphocytes, activate tyrosine kinase pp125FAK, stimulate T cell proliferation and differentiation through the Ras pathway, and enhance cellular immune function, achieving excellent anti-inflammatory effects. It can also stimulate cells to produce nutrients (collagen, small peptides, sodium hyaluronate, etc.), promote cell metabolism, and achieve outstanding anti-aging effects. A specific arrangement of 11 amino acid short peptides connects to fibronectin, guiding macromolecules in the composition into the skin, greatly enhancing the composition's efficacy.

[0009] The combination of the above three components with tetrahydromethylpyrimidine carboxylic acid addresses skin aging and wrinkles from multiple perspectives, and synergistically enhances their anti-aging and anti-wrinkle effects. Performance testing showed that the anti-aging effect was optimal when all four components were present in the composition. The absence of one or two components significantly reduced the composition's anti-aging performance, indicating a synergistic effect among the four components. The decrease was most pronounced when hydrolyzed soybean flour and fibronectin were absent, as these two are core components of the composition.

[0010] Optionally, the method for preparing the hydrolyzed soybean powder is as follows: Mix soybean protein powder with water, heat to 75-90℃ and keep warm for 10-15 minutes to denature it, then cool to 45-65℃ and add compound enzyme to carry out enzymatic hydrolysis for 4-5 hours. During the enzymatic hydrolysis, adjust the pH of the system to 8.8-9.2 with caustic soda. Reheat to 75-90℃ and inactivate for 10-20 minutes. Cool to room temperature, add hydrochloric acid to adjust the pH of the system to 4.3-4.7, centrifuge to remove protein precipitate, and spray dry the supernatant to obtain the final product.

[0011] By adopting the above technical solution, soybean protein isolate (SPI) is moderately hydrolyzed into soybean peptides with smaller molecular weight through enzymatic hydrolysis. By controlling the use of compound enzymes to perform compound hydrolysis of SPI, the degree of hydrolysis can be improved, so that the hydrolyzed soybean powder contains a variety of oligopeptides with a molecular weight of less than 180 Daltons.

[0012] Optionally, the complex enzyme includes alkaline protease, flavor protease, and neutral protease.

[0013] By adopting the above technical solutions, alkaline protease exhibits the strongest hydrolytic ability, with the degree of hydrolysis increasing rapidly in the first 4 hours, followed by a slower rate of increase after 4 hours. Neutral protease is the next strongest, while the hydrolytic ability of flavor protease is significantly affected by the hydrolysis time, with a slow increase in the degree of hydrolysis in the early stages and a substantial increase in the degree of hydrolysis in the later stages.

[0014] Optionally, the method for preparing the hydrolyzed soybean powder is as follows: Mix soybean protein powder with water, heat to 75-85℃ and keep warm for 3-5 minutes, add fucoidan and continue to heat to 90-98℃ and keep warm for 8-12 minutes to obtain the extract. After cooling the extract to 45-65℃, add the compound enzyme for enzymatic hydrolysis for 2.5-3 hours. During the enzymatic hydrolysis, adjust the pH of the system to 8.8-9.2 with food-grade caustic soda. Reheat to 75-90℃ and inactivate for 10-20 minutes. Cool to room temperature, add hydrochloric acid to adjust the pH of the system to 4.3-4.7, remove fucoidan and protein precipitates by passing through a semipermeable membrane, and spray dry the supernatant to obtain the final product.

[0015] By adopting the above technical solution: the new process can significantly improve the enzymatic hydrolysis efficiency by using two-stage heating and adding fucoidan. While reducing the enzymatic hydrolysis time from 4-5 hours to 2.5-3 hours, the DPPH scavenging rate of the composition is still maintained at a high level. The product is still rich in small molecule oligopeptides and has comparable activity and efficacy. The production efficiency is significantly improved and the energy consumption and time cost are reduced.

[0016] The reason for this may be that in traditional processes, denaturation at around 90℃ loosens the three-dimensional structure of proteins, thereby improving enzymatic hydrolysis efficiency. However, in the later stages, protein molecules begin to form network aggregates, leading to a decrease in the hydrolysis rate. Therefore, a hydrolysis time of 4-5 hours is required, which is relatively long. In this application, fucoidan is added to the second denaturation stage at a temperature above 90℃ to improve the degree of hydrolysis of soybean protein, shortening the time while ensuring that it is still rich in various oligopeptides with molecular weights below 180 Daltons.

[0017] Optionally, the amount of fucoidan added is 1-2 parts per ten thousand of the weight of soybean protein powder.

[0018] By adopting the above technical solution, the fucoidan content within the above range effectively ensures the efficiency of proteolytic enzyme hydrolysis, and the anti-wrinkle effect of the composition is at a similar level. Under the premise of ensuring the effect, the waste of raw materials and the burden of subsequent purification caused by excessive addition are avoided.

[0019] Secondly, this application provides a cosmetic product, which adopts the following technical solution: A cosmetic product, wherein the cosmetic product is prepared by adding cosmetic excipients to the multi-effect anti-aging composition described above as the active ingredient, and the amount of the multi-effect anti-aging composition in the cosmetic product is 0.35-1.1 wt%.

[0020] By employing the above technical solution, the aforementioned anti-aging composition with synergistic effects is applied to cosmetics at a specific dosage, enabling the cosmetics to leverage the multi-pathway anti-wrinkle advantages of the composition. However, considering factors such as cost, the dosage of the anti-aging composition should not be excessive.

[0021] Optionally, the cosmetic product is one of the following: skin care water, emulsion, serum, or cream.

[0022] Optionally, the cosmetic is a serum, and the cosmetic excipients include: solvents, chelating agents, moisturizers, thickeners, and antioxidants; The chelating agent is one of disodium EDTA, hydroxyethylidene diphosphonic acid, citric acid, and phytic acid; The moisturizer is one or more of the following: glycerin, propylene glycol, butylene glycol, 1,2-hexanediol, dipropylene glycol, polyethylene glycol-400, trehalose, mannitol, amino acids and their derivatives, and pyrrolidone carboxylates. The thickener is one of sodium hyaluronate and its salts, carbomer, xanthan gum, hydroxyethyl cellulose, and acrylate / C10-30 alkanol acrylate crosspolymers; The antioxidant is one of p-hydroxyacetophenone, tocopherol and its derivatives, ascorbic acid and its derivatives, gallic acid and its esters, ergothioneine, and ferulic acid.

[0023] Optionally, the cosmetic product comprises the following components by weight percentage: Disodium EDTA 0.01-0.05%; Glycerin 5-7%; Sodium hyaluronate 0.1-0.2%; p-Hydroxyacetophenone 0.5-0.6%; 1,2-Hexanediol 0.5-0.6%; Acetylated sodium hyaluronate 0.05-0.5%; Hydrolyzed soybean flour 0.1-0.2%; Tetrahydromethylpyrimidine carboxylic acid 0.1-0.2%; Fibronectin 0.1-0.2%; Water balance.

[0024] By adopting the above technical solution, the ratio of each excipient and active ingredient in the formula is synergistically designed. After efficacy testing, its anti-wrinkle improvement rate can reach up to 41.9%, proving the feasibility and optimality of this specific formula in achieving excellent anti-wrinkle effects.

[0025] Thirdly, this application provides a method for preparing the cosmetic described above, using the following technical solution: A method for preparing the cosmetic described above includes the following steps: S1. Heat water, disodium EDTA, glycerin, sodium hyaluronate, p-hydroxyacetophenone, 1,2-hexanediol and acetylated sodium hyaluronate to 80-90℃ and stir until completely dissolved to form phase A. S2. Cool phase A to 40-50℃, add hydrolyzed soybean powder, tetrahydromethylpyrimidine carboxylic acid and fibronectin, stir evenly to obtain the final product.

[0026] By adopting the above technical solution, the preparation method is simple and involves first dissolving the heat-resistant components at high temperature and then adding the active peptides and proteins at lower temperature. This ensures the uniformity and stability of the system while maximizing the protection of the bioactivity of each active ingredient, especially easily inactivated peptides and fibronectin, thereby ensuring the reliability and consistency of the final product's efficacy.

[0027] In summary, this application has the following beneficial effects: 1. The anti-aging composition of this application exhibits a significant synergistic effect through the scientific combination of acetylated sodium hyaluronate, hydrolyzed soybean powder rich in specific small molecule oligopeptides, tetrahydromethylpyrimidine carboxylic acid, and fibronectin. Performance testing data shows that the complete composition has a DPPH free radical scavenging rate of over 75%, while the scavenging rate drops significantly when any core component is missing, proving that the synergistic effect of the four components is far more than a simple additive effect. Furthermore, the anti-aging performance of this complete composition is significantly superior to traditional peptide combinations, providing a solid chemical basis for its multi-effect anti-wrinkle properties. 2. The preferred method for preparing hydrolyzed soybean powder in this application achieves dual optimization of production efficiency and product activity through two-stage heating and fucoidan-assisted enzymatic hydrolysis. Compared with traditional processes, the new process can reduce the enzymatic hydrolysis time from 4-5 hours to 2.5-3 hours, producing products rich in small molecule oligopeptides with comparable or even slightly better activity, significantly improving production efficiency, reducing production costs, and possessing outstanding industrial application value. 3. This application contains a cosmetic product with an anti-aging composition. After 8 weeks, the wrinkle improvement rate of the essence in this application can reach up to 41.9%, which has an excellent anti-wrinkle effect. Detailed Implementation

[0028] The present application will be further described in detail below with reference to the embodiments. The sources of each component are as follows, and unless otherwise specified, the components are from commercially available products: Disodium EDTA, model number Dissolvine Na2, was sourced from Nourion Chemicals (Ningbo) Co., Ltd. Glycerin, product name Glycerine 99.5% USP, sourced from Emery Oil & Fat Chemicals, Malaysia; Sodium hyaluronate, model Hyaskin® sodium hyaluronate, sourced from Shandong Focus Freda Biotechnology Co., Ltd. Acetylated sodium hyaluronate, model HyaInno®ACE, sourced from Shandong Tiansheng Biotechnology Co., Ltd. p-Hydroxyacetophenone, model number Microcare Antioxidant HAP, sourced from Tor Specialty Chemicals (Zhenjiang) Co., Ltd. Tetrahydromethylpyrimidine carboxylic acid, model ÆCTive® tetrahydromethylpyrimidine carboxylic acid, is sourced from Shandong Tiansheng Biotechnology Co., Ltd. Fibronectin, model MELLPRO-FN recombinant fibronectin, was sourced from MELLPRO (Shenzhen) Biotechnology Co., Ltd. Soy protein powder, manufactured by Wuhan Jiyesheng Chemical Co., Ltd.

[0029] Alkaline protease, flavor protease, and neutral protease are manufactured by Nanning Dongheng Huadao Biotechnology Co., Ltd. Preparation Example 1

[0030] A multi-effect anti-aging composition is obtained by conventional stirring and mixing of 0.05g acetylated sodium hyaluronate, 0.1g hydrolyzed soybean powder, 0.1g tetrahydromethylpyrimidine carboxylic acid and 0.1g fibronectin. The hydrolyzed soybean powder contains various oligopeptides with molecular weights below 180 Daltons, and its preparation method is as follows: Mix 1 kg of soybean protein powder with water at a weight ratio of 1:2, heat to 75℃ and keep warm for 15 min to denature, then cool to 45℃ and add a compound enzyme (the amount of compound enzyme added is 0.05% of the weight of soybean protein powder, and the compound enzyme is composed of alkaline protease, flavor protease and neutral protease in a weight ratio of 4:2.5:3.5) for enzymatic hydrolysis for 5 h. During the enzymatic hydrolysis reaction, the pH of the system is adjusted to 8.8 with food-grade caustic soda. The protein was heated again to 75°C and kept at that temperature for 20 minutes to inactivate it. After cooling to room temperature, hydrochloric acid was added to adjust the pH of the system to 4.3. The protein precipitate was removed by centrifugation, and the supernatant was spray-dried to obtain the final product. Preparation Example 2

[0031] A multi-effect anti-aging composition is obtained by conventional stirring and mixing of 0.3g acetylated sodium hyaluronate, 0.15g hydrolyzed soybean powder, 0.15g tetrahydromethylpyrimidine carboxylic acid and 0.15g fibronectin. The hydrolyzed soybean powder contains various oligopeptides with molecular weights below 180 Daltons, and its preparation method is as follows: Mix 1 kg of soybean protein powder with water at a weight ratio of 1:2, heat to 85℃ and keep warm for 12 min to denature, then cool to 55℃ and add a compound enzyme (the amount of compound enzyme added is 0.05% of the weight of soybean protein powder, and the compound enzyme is composed of alkaline protease, flavor protease and neutral protease in a weight ratio of 4:2.5:3.5) for enzymatic hydrolysis for 4.5 h. During the enzymatic hydrolysis reaction, the pH of the system is adjusted to 9.0 with food-grade caustic soda. The protein was heated again to 80°C and kept at that temperature for 15 minutes to inactivate it. After cooling to room temperature, hydrochloric acid was added to adjust the pH of the system to 4.5. The protein precipitate was removed by centrifugation, and the supernatant was spray-dried to obtain the final product. Preparation Example 3

[0032] A multi-effect anti-aging composition is obtained by conventional stirring and mixing of 0.5g acetylated sodium hyaluronate, 0.2g hydrolyzed soybean powder, 0.2g tetrahydromethylpyrimidine carboxylic acid and 0.2g fibronectin. The hydrolyzed soybean powder contains various oligopeptides with molecular weights below 180 Daltons, and its preparation method is as follows: Mix 1 kg of soybean protein powder with water at a weight ratio of 1:2, heat to 90℃ and keep warm for 10 min to denature, then cool to 65℃ and add a compound enzyme (the amount of compound enzyme added is 0.05% of the weight of soybean protein powder, and the compound enzyme is composed of alkaline protease, flavor protease and neutral protease in a weight ratio of 4:2.5:3.5) for enzymatic hydrolysis for 4 h. During the enzymatic hydrolysis reaction, the pH of the system is adjusted to 9.2 with food-grade caustic soda. The protein was heated again to 90°C and kept at that temperature for 10 minutes to inactivate it. After cooling to room temperature, hydrochloric acid was added to adjust the pH of the system to 4.7. The protein precipitate was removed by centrifugation, and the supernatant was spray-dried to obtain the final product. Preparation Example 4

[0033] A multi-effect anti-aging composition differs from Preparation Example 2 in that the preparation method of the hydrolyzed soybean powder is different. The preparation method of the hydrolyzed soybean powder in this preparation example is as follows: Soy protein powder and water were mixed at a weight ratio of 1:2, heated to 75°C and kept at that temperature for 5 minutes. Fucoidan was then added (the amount of fucoidan added was 1 / 10,000 of the weight of the soybean protein powder), and the temperature was further increased to 90°C and kept at that temperature for 12 minutes to obtain the extract. After cooling the extract to 45°C, add the compound enzyme (the amount of compound enzyme added is 0.05% of the weight of soybean protein powder, and the compound enzyme is composed of alkaline protease, flavor protease and neutral protease in a weight ratio of 4:2.5:3.5) and carry out the enzymatic hydrolysis reaction for 3 hours. During the enzymatic hydrolysis reaction, the pH of the system is adjusted to 8.8 with food-grade caustic soda. The temperature was raised again to 75°C and kept at that temperature for 20 minutes to inactivate the protein. The mixture was then cooled to room temperature, and hydrochloric acid was added to adjust the pH of the system to 4.3. Fucoidan and protein precipitates were removed by passing the mixture through a semipermeable membrane. The supernatant was then spray-dried to obtain the final product. Preparation Example 5

[0034] A multi-effect anti-aging composition differs from Preparation Example 2 in that the preparation method of the hydrolyzed soybean powder is different. The preparation method of the hydrolyzed soybean powder in this preparation example is as follows: Mix soybean protein powder and water at a weight ratio of 1:2, heat to 80℃ and keep warm for 4 minutes, add fucoidan (the amount of fucoidan added is 1 / 10,000 of the weight of soybean protein powder), and continue to heat to 95℃ and keep warm for 10 minutes to obtain the extract. After cooling the extract to 55°C, add the compound enzyme (the amount of compound enzyme added is 0.05% of the weight of soybean protein powder, and the compound enzyme is composed of alkaline protease, flavor protease and neutral protease in a weight ratio of 4:2.5:3.5) and carry out the enzymatic hydrolysis reaction for 2.8 hours. During the enzymatic hydrolysis reaction, the pH of the system is adjusted to 9.0 with food-grade caustic soda. The temperature was raised again to 85°C and kept at that temperature for 15 minutes to inactivate the protein. The mixture was then cooled to room temperature, and hydrochloric acid was added to adjust the pH of the system to 4.5. Fucoidan and protein precipitates were removed by passing the mixture through a semi-permeable membrane. The supernatant was then spray-dried to obtain the final product. Preparation Example 6

[0035] A multi-effect anti-aging composition differs from Preparation Example 2 in that the preparation method of the hydrolyzed soybean powder is different. The preparation method of the hydrolyzed soybean powder in this preparation example is as follows: Soy protein powder and water were mixed at a weight ratio of 1:2, heated to 85°C and kept at that temperature for 3 minutes. Fucoidan was then added (the amount of fucoidan added was 1 / 10,000 of the weight of the soybean protein powder), and the temperature was further increased to 98°C and kept at that temperature for 8 minutes to obtain the extract. After cooling the extract to 65°C, add the compound enzyme (the amount of compound enzyme added is 0.05% of the weight of soybean protein powder, and the compound enzyme is composed of alkaline protease, flavor protease and neutral protease in a weight ratio of 4:2.5:3.5) and carry out the enzymatic hydrolysis reaction for 2.5 hours. During the enzymatic hydrolysis reaction, the pH of the system is adjusted to 9.2 with food-grade caustic soda. The temperature was raised again to 90℃ and kept at that temperature for 10 minutes to inactivate the protein. After cooling to room temperature, hydrochloric acid was added to adjust the pH of the system to 4.7. Fucoidan and protein precipitates were removed by passing the solution through a semi-permeable membrane. The supernatant was then spray-dried to obtain the final product. Preparation Example 7

[0036] A multi-effect anti-aging composition differs from Preparation Example 5 in that the amount of fucoidan added during the preparation of hydrolyzed soybean powder is 1.5 parts per ten thousand of the weight of soybean protein powder. Preparation Example 8

[0037] A multi-effect anti-aging composition differs from Preparation Example 5 in that the amount of fucoidan added during the preparation of hydrolyzed soybean powder is 2 / 10,000 of the weight of soybean protein powder. Comparative preparation examples 1-8

[0038] An anti-aging composition differs from Preparation Example 2 in that the amounts of each component are different, as detailed in the table below.

[0039] Table 1. Comparative analysis of the components and their weights (g) of the anti-aging compositions in Preparation Examples 1-8 Comparative preparation example 9

[0040] An anti-aging composition, which differs from Preparation Example 2 in that an equal amount of polypeptide composition is used instead of hydrolyzed soybean powder; The polypeptide composition is obtained by compounding acetyl hexapeptide-8 and palmitoyl tripeptide-1 in a weight ratio of 1:1. Anti-aging performance test of anti-aging composition

[0041] Referring to TSHRH006-2018, the compositions obtained in the preparation examples and comparative preparation examples were tested and the free radical DPPH scavenging rate was recorded. The higher the scavenging rate, the better the anti-aging and anti-wrinkle effect of the composition. The test results are recorded in the table below.

[0042] Table 2. Results of anti-wrinkle performance test

[0043] Referring to Table 2, the DPPH scavenging rates of Preparation Examples 1-8 in this application, which simultaneously contain acetylated sodium hyaluronate, hydrolyzed soybean powder, tetrahydromethylpyrimidine carboxylic acid, and fibronectin, were all higher than 75%, demonstrating excellent antioxidant capacity, neutralizing harmful free radicals, and preventing cell damage, thus exhibiting superior anti-aging effects. In contrast, the DPPH scavenging rates of Preparation Examples 1-6, lacking one or two of the above four components, decreased significantly, especially in Preparation Example 2 (lacking hydrolyzed soybean powder) and Preparation Example 3 (lacking fibronectin), where the decrease was more drastic. This indicates a synergistic anti-wrinkle effect among the four components, with hydrolyzed soybean protein and fibronectin playing a major role.

[0044] Comparing Preparation Examples 4-6 with Preparation Example 2, the hydrolyzed soybean powder prepared using the new process in Preparation Examples 4-6, especially considering that the new process significantly shortened the enzymatic hydrolysis time from 4-5 hours in the traditional process to 2.5-3 hours, still maintained almost the same DPPH scavenging rate, indicating a better anti-aging effect. In other words, while ensuring comparable product activity, it significantly improved production efficiency and reduced time and energy costs. Example 1

[0045] A cosmetic product, specifically a serum, has the following components and their corresponding weights as shown in Table 3, and is prepared through the following steps: S1. Heat water, disodium EDTA, glycerin, sodium hyaluronate, p-hydroxyacetophenone, 1,2-hexanediol and acetylated sodium hyaluronate to 80°C and stir until completely dissolved to form phase A. S2. Cool phase A to 40°C, add hydrolyzed soybean powder, tetrahydromethylpyrimidine carboxylic acid and fibronectin from Preparation Example 1, and stir until homogeneous to obtain the final product.

[0046] It should be noted that the components in this application can be selected from the following ranges. This selection does not have a significant impact on performance testing. This application only briefly introduces one of them as an example, but it does not affect the application of other components in other embodiments: The chelating agent is one of EDTA disodium, hydroxyethylidene diphosphonic acid, citric acid and phytic acid; the moisturizer is one or more of glycerin, propylene glycol, butylene glycol, 1,2-hexanediol, dipropylene glycol, polyethylene glycol-400, trehalose, mannitol, amino acids and their derivatives and pyrrolidone carboxylates; the thickener is one of sodium hyaluronate and its salts, carbomer, xanthan gum, hydroxyethyl cellulose and acrylate / C10-30 alkanol acrylate crosspolymers; the antioxidant is one of p-hydroxyacetophenone, tocopherol and its derivatives, ascorbic acid and its derivatives, gallic acid and its esters, ergothioneine and ferulic acid. Example 2

[0047] A cosmetic product, specifically a serum, has the following components and their corresponding weights as shown in Table 3, and is prepared through the following steps: S1. Heat water, disodium EDTA, glycerol, sodium hyaluronate, p-hydroxyacetophenone, 1,2-hexanediol and acetylated sodium hyaluronate to 85°C and stir until completely dissolved to form phase A. S2. Cool phase A to 45°C, add hydrolyzed soybean powder, tetrahydromethylpyrimidine carboxylic acid and fibronectin from Preparation Example 2, and stir until homogeneous to obtain the final product. Example 3

[0048] A cosmetic product, specifically a serum, has the following components and their corresponding weights as shown in Table 3, and is prepared through the following steps: S1. Heat water, disodium EDTA, glycerin, sodium hyaluronate, p-hydroxyacetophenone, 1,2-hexanediol and acetylated sodium hyaluronate to 90°C and stir until completely dissolved to form phase A. S2. Cool phase A to 50°C, add hydrolyzed soybean powder, tetrahydromethylpyrimidine carboxylic acid and fibronectin from Preparation Example 3, and stir until homogeneous to obtain the final product. Example 4

[0049] A cosmetic product, specifically an essence, differs from Example 2 in that the hydrolyzed soybean powder used is the same as that prepared in Example 7. Comparative Examples 1-2

[0050] A cosmetic product, specifically a serum, differs from Example 2 in that the amounts of each component are different, as detailed below: Table 3. Components and their weights (g) in Examples 1-3 and Comparative Examples 1-2 Performance testing

[0051] The serums prepared in the examples and comparative examples were subjected to the following performance tests, and the test results are recorded in Table 4.

[0052] Anti-wrinkle performance: Thirty-six individuals with wrinkles and healthy, undamaged skin, aged 35-40, were selected and randomly divided into nine groups of four. Each group used the cosmetics prepared in the examples and comparative studies, applying them morning and evening after facial cleansing. After eight weeks of continuous use, the changes in texture (wrinkle depth, in mm) at the application site were measured using a MICROSKIN II multifunctional dermoscopy image analysis system. The results are recorded in Table 4.

[0053] Improvement rate = (initial average depth - average depth after 8 weeks) / initial average depth × 100%.

[0054] Table 4 Performance Test Results

[0055] Referring to Table 4, in the serums of Examples 1-4, the total amount of acetylated sodium hyaluronate, hydrolyzed soybean powder, tetrahydromethylpyrimidine carboxylic acid, and fibronectin was controlled at 0.35-1.1 wt% of the total serum volume, and the wrinkle improvement rate was found to be higher than 35%. In comparison, Comparative Example 1, with lower amounts of the four components, showed a significant decrease in wrinkle improvement rate, while Comparative Example 2, with amounts exceeding the scope of this application, did not show a significant increase in wrinkle improvement rate but increased raw material costs. Considering factors such as cost, the amounts of each component in Example 2 were optimal.

[0056] The improvement rate of Example 4 was comparable to and slightly better than that of Example 2, indicating that the hydrolyzed soybean powder prepared by the optimized process of Preparation Example 7 can ensure or even slightly improve the core anti-wrinkle efficacy of the final product while shortening the production time.

[0057] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A multi-effect anti-aging composition, characterized in that, The components include the following parts by weight: 0.05-0.5 parts of acetylated sodium hyaluronate; 0.1-0.2 parts of hydrolyzed soybean powder; 0.1-0.2 parts of tetrahydromethylpyrimidine carboxylic acid; Fibronectin 0.1-0.2 parts; The hydrolyzed soybean powder contains a variety of oligopeptides with a molecular weight of less than 180 Daltons.

2. The multi-effect anti-aging composition according to claim 1, characterized in that, The method for preparing the hydrolyzed soybean powder is as follows: Mix soybean protein powder with water, heat to 75-90℃ and keep warm for 10-15 minutes to denature it, then cool to 45-65℃ and add compound enzyme to carry out enzymatic hydrolysis for 4-5 hours. During the enzymatic hydrolysis, adjust the pH of the system to 8.8-9.2 with caustic soda. Reheat to 75-90℃ and inactivate for 10-20 minutes. Cool to room temperature, add hydrochloric acid to adjust the pH of the system to 4.3-4.7, centrifuge to remove protein precipitate, and spray dry the supernatant to obtain the final product.

3. The multi-effect anti-aging composition according to claim 2, characterized in that: The complex enzyme includes alkaline protease, flavor protease, and neutral protease.

4. The multi-effect anti-aging composition according to claim 1, characterized in that, The method for preparing the hydrolyzed soybean powder is as follows: Mix soybean protein powder with water, heat to 75-85℃ and keep warm for 3-5 minutes, add fucoidan and continue to heat to 90-98℃ and keep warm for 8-12 minutes to obtain the extract. After cooling the extract to 45-65℃, add the compound enzyme for enzymatic hydrolysis for 2.5-3 hours. During the enzymatic hydrolysis, adjust the pH of the system to 8.8-9.2 with food-grade caustic soda. Reheat to 75-90℃ and inactivate for 10-20 minutes. Cool to room temperature, add hydrochloric acid to adjust the pH of the system to 4.3-4.7, remove fucoidan and protein precipitates by passing through a semipermeable membrane, and spray dry the supernatant to obtain the final product.

5. The multi-effect anti-aging composition according to claim 4, characterized in that: The amount of fucoidan added is 1-2 parts per ten thousand of the weight of soybean protein powder.

6. A cosmetic product, characterized in that, The cosmetic is prepared by adding cosmetic excipients to the multi-effect anti-aging composition according to any one of claims 1-5 as the active ingredient, and the amount of the multi-effect anti-aging composition in the cosmetic is 0.35-1.1 wt%.

7. A cosmetic product according to claim 6, characterized in that: The cosmetic product is one of the following: skin care water, emulsion, serum, or cream.

8. A cosmetic product according to claim 6, characterized in that: The cosmetic is an essence, and the cosmetic excipients include: solvents, chelating agents, moisturizers, thickeners, and antioxidants; The chelating agent is one of disodium EDTA, hydroxyethylidene diphosphonic acid, citric acid, and phytic acid; The moisturizer is one or more of the following: glycerin, propylene glycol, butylene glycol, 1,2-hexanediol, dipropylene glycol, polyethylene glycol-400, trehalose, mannitol, amino acids and their derivatives, and pyrrolidone carboxylates. The thickener is one of sodium hyaluronate and its salts, carbomer, xanthan gum, hydroxyethyl cellulose, and acrylate / C10-30 alkanol acrylate crosspolymers; The antioxidant is one of p-hydroxyacetophenone, tocopherol and its derivatives, ascorbic acid and its derivatives, gallic acid and its esters, ergothioneine, and ferulic acid.

9. A cosmetic product according to claim 8, characterized in that: The cosmetic product is composed of the following components by weight percentage: Disodium EDTA 0.01-0.05%; Glycerin 5-7%; Sodium hyaluronate 0.1-0.2%; p-Hydroxyacetophenone 0.5-0.6%; 1,2-Hexanediol 0.5-0.6%; Acetylated sodium hyaluronate 0.05-0.5%; Hydrolyzed soybean flour 0.1-0.2%; Tetrahydromethylpyrimidine carboxylic acid 0.1-0.2%; Fibronectin 0.1-0.2%; Water balance.

10. A method for preparing the cosmetic according to claim 9, characterized in that: Includes the following steps: S1. Heat water, disodium EDTA, glycerin, sodium hyaluronate, p-hydroxyacetophenone, 1,2-hexanediol and acetylated sodium hyaluronate to 80-90℃ and stir until completely dissolved to form phase A. S2. Cool phase A to 40-50℃, add hydrolyzed soybean powder, tetrahydromethylpyrimidine carboxylic acid and fibronectin, stir evenly to obtain the final product.

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