Antibody of campylobacter specific protein, antibody coupling magnetic bead and application

By preparing monoclonal antibodies 1A10 and 3D9, which are specific proteins of Campylobacter genus CadF and CDTB, and conjugating them with magnetic beads, the problem of enriching and isolating Campylobacter genus microorganisms in existing technologies has been solved, achieving efficient and low-cost microbial enrichment and analysis.

CN122103326APending Publication Date: 2026-05-29MEIYITIAN BIOMEDICAL (NINGBO) CO LTD
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Patent Information

Application Number
CN202411703057.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-26
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing technologies are insufficient for the efficient enrichment and isolation of Campylobacter microorganisms, especially those strains that are difficult to culture, leading to difficulties in microbial detection and analysis.

Method used

Monoclonal antibodies 1A10 and 3D9 were prepared using Campylobacter-specific proteins CadF and CDTB, and then conjugated with magnetic beads to form antibody-conjugated magnetic beads. Campylobacter was then enriched by magnetic field sorting.

Benefits of technology

It achieves efficient enrichment and isolation of Campylobacter spp., reduces costs, and eliminates the need for expensive instruments, making it suitable for qualitative and quantitative analysis of Campylobacter spp.

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Abstract

The invention discloses a campylobacter specific protein antibody, an antibody coupling magnetic bead and application. The antibodies are a monoclonal antibody 1A10 and a monoclonal antibody 3D9, the monoclonal antibody 1A10 comprises a light chain variable region and a heavy chain variable region, and the amino acid sequences of the light chain variable region and the heavy chain variable region of the monoclonal antibody 1A10 are respectively shown as SEQ ID NO: 7 and 8; the monoclonal antibody 3D9 comprises a light chain variable region and a heavy chain variable region, and the amino acid sequences of the light chain variable region and the heavy chain variable region of the monoclonal antibody 3D9 are respectively shown as SEQ ID NO: 9 and 10. The antibody coupling magnetic bead is formed by respectively coupling a monoclonal antibody 1A10 and a monoclonal antibody 3D9 with an activated carboxyl magnetic bead. The antibody coupled magnetic bead can specifically enrich campylobacter, is high in enrichment efficiency and simple in enrichment operation, can be applied to analysis of metabolites of campylobacter, and can also be used for qualitative and quantitative analysis of campylobacter.
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