Bovine type ii interferon-ferritin fusion protein, mutant thereof, and preparation method and application thereof

By fusing bovine type II gamma interferon with the ferritin subunit and performing a mutation to enhance its antiviral activity, the problems of low activity and poor stability of bovine type II gamma interferon in viral diseases have been solved, achieving highly effective prevention or treatment of viral diseases, making it suitable for application in the cattle farming industry.

CN122103378APending Publication Date: 2026-05-29THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Filing Date
2026-04-29
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Bovine type II gamma interferon has low antiviral activity, poor in vivo stability, and a short half-life in the prevention or treatment of viral diseases, which limits its application.

Method used

Bovine type II gamma interferon and ferritin subunits are linked by a flexible linker to form a fusion protein. Single-site or multi-site mutations are then performed to enhance antiviral activity. Ferritin nanoparticles are used to increase the in vivo retention time, and the protein is then mass-produced using a silkworm baculovirus expression system.

Benefits of technology

It significantly enhances the antiviral activity and in vivo stability of bovine type II gamma interferon, prolongs its half-life, provides a highly effective drug for the prevention or treatment of viral diseases, simplifies the administration process, and is suitable for use in the cattle farming industry.

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Abstract

The application discloses a bovine type II gamma-interferon-ferritin fusion protein, a mutant thereof, and a preparation method and application thereof. The bovine type II gamma-interferon and a ferritin subunit are connected through a flexible peptide to realize surface display of the interferon protein, form a dimer conformation suitable for the bovine type II gamma-interferon to play a function, effectively improve the expression amount and activity of the bovine type II gamma-interferon, improve the stability of the bovine type II gamma-interferon in the body, and prolong the half-life period. The fusion protein is further rationally designed to obtain a mutant with significantly improved antiviral activity and stability. The application adopts a silkworm or insect cell eukaryotic expression system to express the fusion protein or the mutant, the expression system is safe to operate, simple in procedure, low in cost, and extremely beneficial to large-scale industrial production; and the prepared fusion protein or mutant can be applied to the preparation of a medicine for preventing or treating bovine viral infection, tumor, and immune system diseases, and an immune adjuvant for vaccine compounding.
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Citation Information

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