A mitochondrial function optimization additive for cell culture, a preparation method and application thereof

The mitochondrial function optimization additive, composed of D-Arg-Cha-Lys-Phe-NH2 peptide, ubiquinone-10 phosphate, reduced glutathione, and α-lipoic acid, solves the problem of mitochondrial damage and achieves the stability, morphology maintenance, membrane potential maintenance, and ROS scavenging of mitochondrial cardiolipin, thereby improving cell culture efficiency and quality.

CN122104549BActive Publication Date: 2026-07-24SHANDONG QUANGANG BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANDONG QUANGANG BIOTECHNOLOGY CO LTD
Filing Date
2026-04-29
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

In existing cell cultures, mitochondria are easily damaged, and protective agents have limited functions, failing to simultaneously achieve mitochondrial cardiolipin stabilization, morphology maintenance, membrane potential preservation, and mitochondrial ROS clearance, resulting in decreased cell viability and low culture efficiency.

Method used

A mitochondrial function-optimizing additive composed of D-Arg-Cha-Lys-Phe-NH2 peptide, ubiquinone-10 phosphate, reduced glutathione, and α-lipoic acid binds to mitochondrial cardiolipin through electrostatic and hydrophobic interactions, participates in electron transport, scavenge ROS, forms a synergistic antioxidant system, and stabilizes membrane potential and morphology.

Benefits of technology

It significantly increases mitochondrial cardiolipin content by more than 50%, reduces the proportion of abnormal mitochondria to ≤12%, increases membrane potential positive cells to ≥86%, reduces mitochondrial ROS by more than 40%, increases cell proliferation rate by more than 25%, and has no cytotoxicity.

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Abstract

The application provides a kind of mitochondrial function optimization additive for cell culture, preparation method and application, belongs to cell culture technical field.The additive includes core active ingredient, auxiliary component and carrier, and the core active ingredient includes D-Arg-Cha-Lys-Phe-NH2 Polypeptide, ubiquinone-10 phosphate, reduced glutathione and alpha-lipoic acid.The application forms a multi-target core active system through the synergy of the four, realizes the triple synergistic protection of stabilizing mitochondrial cardiolipin, maintaining mitochondrial morphology and membrane potential, and reducing mitochondrial ROS, which can significantly improve cell culture quality.When applied, the mitochondrial cardiolipin content can be increased by more than 50%, the mitochondrial abnormality ratio can be reduced to less than 12%, the membrane potential positive cell ratio can be increased to more than 86%, the mitochondrial ROS level can be reduced by more than 40%, and the cell proliferation rate can be increased by more than 25%, which is suitable for in vitro culture of various eukaryotic cells, and the preparation process is simple and has no cytotoxicity.
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Citation Information

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