A method for screening and preparing a low-temperature resistant vibrio bacteriophage

Abstract

The application discloses a kind of screening and preparation method of low temperature resistant vibrio phage, the vibrio phage sample solution to be screened is introduced into screening container, after the feeding of multiple groups of screening containers is completed in batches, four groups of screening containers are aligned with four groups of independent temperature adjusting regions, and the sealing docking of screening container and liquid discharge structure is completed;Compressed air is generated and injected into four groups of airflow generating structures after shunting structure to generate cold air and hot air, wherein the cold air is introduced into four groups of independent temperature adjusting regions through shunting pipeline respectively, and the application relates to the technical field of phage screening and preparation.The screening and preparation method of low temperature resistant vibrio phage allows the second motor to cooperate with the second gear, the second gear ring, adjusts the angle of support seat, rotating seat, heat preservation plate and screening shell, and aligns the temperature adjusting cavity of the corresponding temperature adjusting section of the first temperature adjusting ring and the second temperature adjusting ring by adjusting the angle.

Description

Technical Field

[0001] This invention relates to the field of phage screening and preparation technology, specifically to a method for screening and preparing low-temperature resistant Vibrio phage. Background Technology

[0002] In the screening and preparation of low-temperature resistant Vibrio phages, appropriate equipment is required to screen, purify, enrich, and prepare the low-temperature resistant Vibrio phages. Referring to the enrichment screening mechanism and its preparation method in publication number CN110885742B, the enrichment screening mechanism includes multiple parallel porous mesh substrates. The porous mesh substrate includes a mesh substrate formed by multiple intersecting mesh lines, multiple sieve holes formed by the multiple intersecting mesh lines, and a capture layer formed on the mesh substrate.

[0003] As shown in the aforementioned patent, most existing methods for screening and preparing low-temperature resistant Vibrio phages rely on traditional single-layer screening structures. These methods can only perform screening operations under a single temperature condition and cannot simultaneously provide multiple low-temperature environments with different gradients. This makes it difficult to efficiently screen out low-temperature resistant Vibrio phages adapted to different low-temperature scenarios. Furthermore, the temperature fluctuates significantly during the screening process, and the temperature control precision is insufficient, which can easily lead to damage to phage activity, affecting the screening effect and preparation purity. At the same time, existing screening devices mostly adopt fixed screening structures, requiring manual operation of steps such as feeding, screening, draining, and cleaning. This process is cumbersome, time-consuming, and labor-intensive, making it difficult to achieve batch continuous operation. The screening and preparation efficiency is low and cannot meet the needs of large-scale production. In addition, although some screening devices are equipped with multiple screening units, there is a lack of effective heat insulation and isolation structures between the screening units, which can easily lead to temperature cross-interference, resulting in inconsistent screening conditions among the groups and failing to guarantee the accuracy and stability of the screening results. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a method for screening and preparing low-temperature resistant Vibrio phages, which solves the problems of existing screening methods being unable to simultaneously provide multiple independent gradient low-temperature environments, insufficient temperature control accuracy, easy damage to phage activity, and cumbersome operation of screening devices, making it impossible to achieve batch continuous operation.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a method for screening and preparing low-temperature resistant Vibrio phages, specifically comprising the following steps:

[0006] Step 1: Import the Vibrio phage sample solution to be screened into the screening container. After loading multiple sets of screening containers in batches, align four sets of screening containers with four independent temperature control areas, and complete the sealing connection between the screening containers and the drainage structure.

[0007] Step 2: The air compressor generates compressed air, which is injected into four sets of vortex tubes through a splitting structure to produce cold air and hot air. The cold air is introduced into four independent temperature-controlled zones through the splitting pipes. Through heat exchange, the Vibrio phage solution in the four screening containers is independently regulated to maintain the screening temperature in a low temperature range of 0℃ to 12℃. At the same time, the Vibrio phage sample solution in the screening containers is stirred to promote the full progress of the screening reaction.

[0008] Step 3: After the screening operation is completed, the solutions in the four screening containers are extracted to the corresponding low-temperature ultrafiltration concentration units through the drainage structure for low-temperature concentration treatment. After concentration, the solutions are purified and finally the low-temperature resistant Vibrio phages obtained from the screening are stored.

[0009] Preferably, in step two, each group of airflow generating structures is equipped with a set of regulating components. The flow rate at the hot air emission end of the airflow generating structure is independently controlled by the regulating components, thereby regulating the temperature at the cold air emission end of the airflow generating structure and ensuring that the temperatures of the four independent temperature-regulating zones do not interfere with each other.

[0010] This invention also discloses a device for screening and preparing low-temperature resistant Vibrio phages. The device includes a housing, and the housing is connected to a processing component for screening and preparing low-temperature resistant Vibrio phages. The processing component includes:

[0011] The feeding component, installed on the upper side of the shell, is used for feeding low-temperature resistant Vibrio phage samples;

[0012] An array of support feet is fixedly connected to the bottom edge of the housing to provide support;

[0013] A storage unit, installed on a housing, is used to store and regulate low-temperature resistant Vibrio phage samples discharged from a feeding unit. The storage unit includes a rotating seat rotatably connected to the inside of the housing. Six sets of screening shells are fixedly connected to the upper edge of the rotating seat. A temperature sensor that fits against the lower end of the screening shell is fixedly connected to the rotating seat. A set of insulation plates is fixedly connected to the rotating seat near both sides of the screening shell. A stirring component for stirring the samples in the six sets of screening shells is installed on the lower side of the rotating seat. A driving component for driving the rotating seat to rotate is fixedly connected to the housing. A draining component for draining liquid from the screening shell is connected to the driving component. A docking component for assisting the draining component in draining liquid is installed on the lower side of the housing.

[0014] Adjustment components, installed on the housing, are used to regulate the temperature of the screening shell;

[0015] The cleaning component is installed on the upper rear side of the housing for cleaning the screening shell.

[0016] Preferably, the cleaning component includes a water pump fixedly connected to the housing, a spray plate fixedly connected to the upper side of the housing near a set of screening shells, a conduit fixedly connected to the drain end of the water pump, a first solenoid valve fixedly connected to the upper side of the spray plate and fixedly connected to the conduit, and a pumping pipe fixedly connected to the pumping end.

[0017] Preferably, the adjusting component includes a first temperature regulating ring and a second temperature regulating ring fixedly connected to the top of the inner side of the housing. The outer wall of the first temperature regulating ring is slidably connected to the insulation plate and the screening shell, respectively. The inner wall of the second temperature regulating ring is slidably connected to the insulation plate and the screening shell, respectively. The first temperature regulating ring and the second temperature regulating ring are aligned with the axis of the rotating seat and are respectively provided with four sets of non-communicating temperature regulating cavities on their inner sides. Four sets of second branch pipes and third branch pipes are fixedly connected to the four sets of temperature regulating cavities between the first temperature regulating ring and the second temperature regulating ring. A second solenoid valve is fixedly connected to the second branch pipe. A vortex tube for generating cold air is fixedly connected to the end of the second solenoid valve away from the housing. The air inlet ends of the four sets of vortex tubes are fixedly connected to a first branch pipe. An air compressor is fixedly connected to the front side of the first branch pipe. A flow valve is fixedly connected to the side of the vortex tube away from the second solenoid valve.

[0018] Preferably, the portions of the first and second temperature regulating rings near the four sets of temperature regulating cavities are made of aluminum alloy. Four sets of heat insulation plates are installed on the inner side of the first and second temperature regulating rings between the four sets of temperature regulating cavities. The heat insulation plates are used to separate the four sets of temperature regulating cavities from each other to ensure that the temperatures of each set of temperature regulating cavities do not interfere with each other. The bottom of the first and second temperature regulating rings is attached to the upper end of the rotating seat. The second and third branch pipes are both sealed and connected to the temperature regulating cavities.

[0019] Preferably, the stirring component includes six sets of stirring rods that are rotatably connected to six sets of screening shells respectively. A first gear is coaxially fixedly connected to the stirring rod near the lower end of the rotating seat. The six sets of first gears mesh together with a set of first gear rings that are rotatably connected to the lower edge of the rotating seat. A first motor is fixedly connected to the lower right end of the rotating seat. The first motor is coaxially fixedly connected to a set of first gears.

[0020] Preferably, the driving component includes a support base coaxially fixedly connected to the bottom of the rotating seat and rotatably connected to the housing. A second gear ring is coaxially fixedly connected to the lower side of the support base. A second motor is fixedly connected to the lower left end of the housing. A second gear meshing with the second gear ring is coaxially fixedly connected to the upper output end of the second motor. A fourth branch pipe connected to the draining component is fixedly connected to the middle part of the support base. A draining pipe rotatably connected to and communicating with the bottom of the fourth branch pipe is fixedly connected to the middle end of the bottom of the housing.

[0021] Preferably, the draining component includes a third solenoid valve fixedly connected to the lower end of the screening shell. The bottom of the third solenoid valve is fixedly connected to a three-way valve for switching the liquid flow direction and connected to the drive component. The bottom of the three-way valve is fixedly connected to a discharge pipe. The bottom of the discharge pipe is fixedly connected to a first docking ring. The docking component includes a guide frame slidably connected to the bottom of the shell. The lower side of the shell is fixedly connected to a cylinder for adjusting the height of the guide frame. The upper side of the guide frame is fixedly connected to six sets of fourth solenoid valves corresponding to the lower side of the six screening shells. The upper end of the fourth solenoid valve is fixedly connected to a second docking ring that matches the first docking ring. The bottom of the fourth solenoid valve is fixedly connected to a bellows. The bottom of the bellows is fixedly connected to an outlet pipe disposed at the bottom of the shell and used for discharging the phage solution.

[0022] Preferably, the second docking ring has a docking groove on its upper side that matches the first docking ring and a sealing element on its edge; the discharge pipe is connected to an external material extraction component; and the extended end of the cylinder is fixedly connected to the guide frame.

[0023] Beneficial effects

[0024] This invention provides a method for screening and preparing low-temperature resistant Vibrio phages. Compared with existing technologies, it has the following advantages:

[0025] (1) The method for screening and preparing low-temperature resistant Vibrio phages involves setting up adjustment and storage components, allowing the second motor, second gear, and second gear ring to cooperate in adjusting the angles of the support base, rotating base, insulation plate, and screening shell. This facilitates the feeding component to feed the screening shells in sequence. By adjusting the angles, the screening shells that have been fed in the corresponding number of groups are aligned with the temperature control chambers of the corresponding temperature control sections of the first and second temperature control rings, which helps in subsequent temperature control operations. The air compressor, first branch pipe, four sets of vortex pipes, second solenoid valve, second branch pipe, third branch pipe, and flow valve cooperate to provide cold air of the corresponding temperature to the temperature control chambers of the corresponding sections. Through heat transfer and heat absorption, different low-temperature environments are provided for the screening shells at different positions, which helps in the screening of low-temperature resistant Vibrio phages under different low-temperature environments. The first motor, first gear ring, first gear, and stirring rod cooperate to centrifuge the Vibrio phage solution in the corresponding low-temperature environment inside the screening shell, accelerating temperature control and Vibrio phage screening.

[0026] (2) The method for screening and preparing low-temperature Vibrio phages involves setting up a cleaning component. After the solution is discharged from the array of screening shells, the liquid direction of the three-way valve is changed. By adjusting the angle of the rotating seat, the heat preservation plate, and the screening shell, the water pump, the conduit, the first solenoid valve, and the spray plate work together to wash the screening shell and the stirring rod that pass under the spray plate. The impurities on the inner wall of the screening shell and the surface of the stirring rod are discharged to the external waste liquid recovery end through the third solenoid valve, the three-way valve, the fourth branch pipe, and the drain pipe under the flushing of water, thereby improving the cleaning efficiency of the storage end and reducing the impact on the subsequent screening and preparation operations of low-temperature Vibrio phages.

[0027] (3) The screening and preparation method of the low-temperature Vibrio phage is achieved by setting a docking component. After the angle of the array screening shell is adjusted, the first docking ring and the second docking ring on the lower side of the screening shell are aligned. The guide frame, the fourth solenoid valve, and the height of the second docking ring are adjusted by the cylinder so that the first docking ring is inserted into the docking groove in the second docking ring. When it is necessary to export the solution after low-temperature treatment, the corresponding third solenoid valve and the fourth solenoid valve are opened so that the external material extraction component can extract the solution through the third solenoid valve, the three-way valve, the discharge pipe, the first docking ring, the second docking ring, the bellows, and the discharge pipe. This facilitates the material extraction component to inject the solution into the low-temperature ultrafiltration concentration unit for low-temperature filtration treatment. This setting does not affect the adjustment of the low-temperature Vibrio phage storage end, and has the function of exporting the low-temperature Vibrio phage after the screening operation is completed. There is no need to manually remove the preparation solution, which improves the screening and preparation efficiency. Attached Figure Description

[0028] Figure 1 This is a three-dimensional structural view of the present invention;

[0029] Figure 2 This is a partial enlarged cross-sectional view of the structure of the present invention;

[0030] Figure 3 This is an enlarged view of the cleaning component of the present invention;

[0031] Figure 4 This is a partial enlarged cross-sectional view of the adjusting component of the present invention;

[0032] Figure 5 This is a partial enlarged cross-sectional view of the storage component of the present invention;

[0033] Figure 6 This is an enlarged view of the stirring component of the present invention;

[0034] Figure 7 This is a partial enlarged cross-sectional view of the driving component of the present invention;

[0035] Figure 8 This is a partially enlarged view of the draining component of the present invention;

[0036] Figure 9This is an enlarged view of the docking component of the present invention.

[0037] In the diagram: 1. Shell; 2. Feeding component; 3. Cleaning component; 31. Water pump; 32. Pipe; 33. First solenoid valve; 34. Spray plate; 4. Support leg; 5. Adjusting component; 51. Air compressor; 52. First branch pipe; 53. Vortex tube; 54. Second solenoid valve; 55. Second branch pipe; 56. First temperature regulating ring; 57. Second temperature regulating ring; 58. Third branch pipe; 59. Flow valve; 6. Storage component; 61. Rotating seat; 62. Insulation plate; 63. Screening shell; 64. Agitator; 641. First motor; 642. 643. First gear; 644. First gear ring; 645. Stirring rod; 65. Driving component; 651. Support base; 652. Second motor; 653. Second gear; 654. Second gear ring; 655. Fourth branch pipe; 656. Drain pipe; 66. Drain component; 661. Third solenoid valve; 662. Three-way valve; 663. Discharge pipe; 664. First docking ring; 67. Docking component; 671. Cylinder; 672. Guide frame; 673. Fourth solenoid valve; 674. Second docking ring; 675. Bellows; 676. Discharge pipe. Detailed Implementation

[0038] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0039] Please refer to Figures 1-9 A method for screening and preparing low-temperature resistant Vibrio phages, specifically including the following steps:

[0040] Step 1: The Vibrio phage sample solution to be screened is introduced into a set of screening shells 63 through the feeding component 2. After the Vibrio phage sample solution in the set of screening shells 63 is added, the second motor 652 drives the second gear 653 to rotate. The rotating second gear 653 meshes with the second gear ring 654, driving the support base 651, the fourth branch pipe 655, the rotating base 61, the stirring component 64, the six sets of screening shells 63, and the twelve sets of insulation plates 62 to rotate. The insulation plates 62 slide against the outer wall of the first temperature regulating ring 56 and the inner wall of the second temperature regulating ring 57, respectively. After the screening shells 63 without Vibrio phage sample solution are moved to the lower side of the feeding component 2, the adjustment of the angle of the support base 651 is paused, and the adjustment of the screening shells continues. 63. Feeding is performed, and the feeding operation is repeated. After the four sets of screening shells 63 that need to be fed are fed, the position of the four sets of screening shells 63 is adjusted so that the four sets of screening shells 63 are aligned with the areas of the four corresponding temperature-adjusting chambers formed by the first temperature-adjusting ring 56 and the second temperature-adjusting ring 57. At this time, the first docking ring 664 on the lower side of the six sets of screening shells 63 is aligned with the six sets of second docking rings 674. The height of the guide frame 672, the fourth solenoid valve 673, and the second docking ring 674 is adjusted by the cylinder 671 so that the second docking ring 674 is docked with the first docking ring 664. The heat insulation plates 62 on both sides of the screening shells 63 play a heat preservation role. The four sets of flow valves 59 are adjusted to control the flow rate of the hot gas discharge end of the four sets of vortex tubes 53.

[0041] Step 2: Open the four sets of second solenoid valves 54 to allow the air compressor 51 to draw in and compress outside air. The compressed air is then injected into the four sets of vortex tubes 53 via the first branch pipe 52. The vortex tubes 53 convert the compressed air into cold and hot air through vortex conversion. The flow valve 59 regulates the flow rate of the hot air, thereby regulating the temperature of the cold and hot air. The cold air is then introduced into the corresponding temperature-regulating chambers in the first temperature-regulating ring 56 and the second temperature-regulating ring 57 via the second branch pipe 55. The first temperature-regulating ring 56 and the second temperature-regulating ring 57 regulate the temperature of the corresponding chambers through heat exchange. The screening shell 63 and Vibrio phage solution in the temperature-regulating chamber area are subjected to heat absorption and cooling treatment. The temperature of the screening shell 63 is detected by the temperature sensor on the lower side of the screening shell 63 to ensure that the four sets of screening shells 63 in different positions are at a suitable low temperature. The first motor 641, the first gear ring 643 and the array of first gears 642 work together to drive the array of stirring rods 644. The four rotating stirring rods 644 stir the Vibrio phage sample solution in the four sets of screening shells 63 at a suitable low temperature, thereby achieving the screening of Vibrio phage samples.

[0042] Step 3: In Step 2, the hot air is discharged to the external heat recovery end through the flow valve 59 and the heat exhaust pipe. The cold air entering the first temperature control ring 56 and the second temperature control ring 57 is finally discharged to the external cold air recovery end through the third branch pipe 58. After the screening operation is completed, the air compressor 51 and the second solenoid valve 54 are turned off, and the third solenoid valve 661 and the fourth solenoid valve 673 are turned on to open the external material extraction equipment. The solution in the four screening shells 63 is extracted by the external material extraction equipment through the third solenoid valve 661, the three-way valve 662, the discharge pipe 663, the first docking ring 664, the second docking ring 674, the fourth solenoid valve 673, the bellows 675, and the discharge pipe 676 and introduced into the corresponding low-temperature ultrafiltration concentration unit for low-temperature concentration treatment. Then, the solution after low-temperature concentration treatment is purified and finally stored in the corresponding refrigerated storage cabinet containing the screened low-temperature resistant Vibrio phage.

[0043] Please refer to Figures 1-9 The present invention also discloses a device for screening and preparing low-temperature resistant Vibrio phages, specifically providing the following three technical solutions:

[0044] First embodiment: A device for screening and preparing low-temperature resistant Vibrio phage, the device includes a shell 1, the shell 1 is connected to a processing component for screening and preparing low-temperature resistant Vibrio phage, the processing component includes: a feeding component 2, installed on the upper side of the shell 1 for feeding low-temperature resistant Vibrio phage samples; and an array of support feet 4, fixedly connected to the bottom edge of the shell 1 for providing support.

[0045] Storage component 6, installed on housing 1, is used to store and regulate the low-temperature resistant Vibrio phage samples discharged from feeding component 2. Storage component 6 includes a rotating seat 61 rotatably connected to the inside of housing 1. Six sets of screening shells 63 are fixedly connected to the upper edge of rotating seat 61. A temperature sensor that fits against the lower end of screening shell 63 is fixedly connected to rotating seat 61. A set of heat preservation plates 62 are fixedly connected to both sides of rotating seat 61 near screening shell 63. A stirring component 64 for stirring the samples in the six sets of screening shells 63 is installed on the lower side of rotating seat 61. A driving component 65 for driving rotating seat 61 is fixedly connected to housing 1. A drain component 66 for draining liquid from screening shell 63 is connected to driving component 65. A docking component 67 for assisting draining component 66 is installed on the lower side of housing 1. Adjustment component 5, installed on housing 1, is used to adjust the temperature of screening shell 63. Cleaning component 3, installed on the upper rear end of housing 1, is used to clean screening shell 63.

[0046] Adjusting component 5 includes a first temperature-regulating ring 56 and a second temperature-regulating ring 57 fixedly connected to the top inner side of housing 1. The outer wall of the first temperature-regulating ring 56 is slidably connected to the insulation plate 62 and the screening shell 63, respectively. The inner wall of the second temperature-regulating ring 57 is slidably connected to the insulation plate 62 and the screening shell 63, respectively. The first temperature-regulating ring 56 and the second temperature-regulating ring 57 are aligned with the axis of the rotating seat 61 and have four sets of non-communicating temperature-regulating cavities on their inner sides. Four sets of second temperature-regulating rings are fixedly connected to the four sets of temperature-regulating cavities corresponding to the first temperature-regulating ring 56 and the second temperature-regulating ring 57. The second branch pipe 55 is fixedly connected to the second solenoid valve 54. The end of the second solenoid valve 54 away from the housing 1 is fixedly connected to the vortex pipe 53 for generating cold air. The air inlet ends of the four sets of vortex pipes 53 are fixedly connected to the first branch pipe 52. The front side of the first branch pipe 52 is fixedly connected to the air compressor 51. The side of the vortex pipe 53 away from the second solenoid valve 54 is fixedly connected to the flow valve 59. The flow valve 59 is connected to the external heat recovery end. The third branch pipe 58 is connected to the external cold air recovery end.

[0047] The first temperature regulating ring 56 and the second temperature regulating ring 57 are made of aluminum alloy near the four temperature regulating chambers. Four sets of PIR polyurethane rigid heat insulation plates are installed on the inner side of the first temperature regulating ring 56 and the second temperature regulating ring 57 between the four temperature regulating chambers. The bottom of the first temperature regulating ring 56 and the second temperature regulating ring 57 are attached to the upper end of the rotating seat 61. The air compressor 51 is equipped with a filter screen at the air extraction end. The second branch pipe 55 and the third branch pipe 58 are sealed and connected to the temperature regulating chamber. The stirring component 64 includes six stirring rods 644 that are rotatably connected to the six sets of screening shells 63. The stirring rods 644 are coaxially fixedly connected to the lower end of the rotating seat 61 with a first gear 642. The six sets of first gears 642 mesh together with a first toothed ring 643 that is rotatably connected to the lower edge of the rotating seat 61. The lower right end of the rotating seat 61 is fixedly connected to a first motor 641. The first motor 641 is coaxially fixedly connected to a set of first gears 642.

[0048] The driving component 65 includes a support base 651 coaxially fixedly connected to the bottom of the rotating seat 61 and rotatably connected to the housing 1. A second gear ring 654 is coaxially fixedly connected to the lower side of the support base 651. A second motor 652 is fixedly connected to the lower left end of the housing 1. A second gear 653 that meshes with the second gear ring 654 is coaxially fixedly connected to the upper output end of the second motor 652. A fourth branch pipe 655 connected to the drain component 66 is fixedly connected to the middle of the support base 651. A fourth branch pipe 655 is fixedly connected to the middle of the bottom of the housing 1. A drain pipe 656 is rotatably connected to and communicates with the bottom of the fourth branch pipe 655; the drain component 66 includes a third solenoid valve 661 fixedly connected to the lower end of the screening shell 63, a three-way valve 662 for switching the liquid flow direction and connected to the drive component 65 is fixedly connected to the bottom of the third solenoid valve 661, the other drain end of the three-way valve 662 is fixedly connected to the fourth branch pipe 655, a discharge pipe 663 is fixedly connected to the bottom of the three-way valve 662, and a first docking ring 664 is fixedly connected to the bottom of the discharge pipe 663;

[0049] The second motor 652, second gear 653, and second gear ring 654 work together to adjust the angles of the support base 651, rotating base 61, insulation plate 62, and screening shell 63, thereby facilitating the feeding component 2 to feed the array of screening shells 63 sequentially. By adjusting the angles, the corresponding number of screening shells 63 are aligned with the temperature regulating chambers of the corresponding temperature regulating sections of the first temperature regulating ring 56 and the second temperature regulating ring 57. The air compressor 51, first branch pipe 52, four sets of vortex pipes 53, second solenoid valve 54, second branch pipe 55, third branch pipe 58, and flow valve 59 work together to provide cold air of the corresponding temperature to the temperature regulating chambers of the corresponding sections, thereby providing different low-temperature environments for the screening shells 63 at different positions. The first motor 641, first gear ring 643, first gear 642, and stirring rod 644 work together to centrifuge the Vibrio phage solution in the corresponding low-temperature environment inside the screening shell 63.

[0050] The main difference between the second implementation method and the first implementation method is that:

[0051] Cleaning component 3 includes a water pump 31 fixedly connected to the housing 1. A spray plate 34 is fixedly connected to the upper side of the housing 1 near a set of screening shells 63. A conduit 32 is fixedly connected to the drain end of the water pump 31. A first solenoid valve 33, which is fixedly connected to the conduit 32, is fixedly connected to the upper side of the spray plate 34. The spray plate 34 has the same shape as the upper opening of the screening shell 63 and has several spray holes at the bottom. A suction pipe is fixedly connected to the suction end of the water pump 31. After the set of screening shells 63 has finished draining the screening solution, the three-way valve 662 is reversed, allowing the drive component 65 to continue adjusting the rotating seat 61 and the insulation plate 62. The angle of the screening shell 63 is adjusted so that the screening shell 63 to be cleaned is moved to the lower side of the water spray plate 34. The first solenoid valve 33 and the water pump 31 are turned on. The water pump 31 draws clean water from the outside through the water pumping pipe and washes the screening shell 63 and the stirring rod 644 inside through the conduit 32, the first solenoid valve 33 and the water spray plate 34. The impurities on the inner wall of the screening shell 63 and the surface of the stirring rod 644 are discharged to the external waste liquid recovery end through the third solenoid valve 661, the three-way valve 662, the fourth branch pipe 655 and the drain pipe 656 under the washing of water. The remaining screening shells 63 and stirring rods 644 are cleaned in sequence.

[0052] The main difference between the third and second implementation methods is that:

[0053] The docking component 67 includes a guide frame 672 that is slidably connected to the bottom of the housing 1. A cylinder 671 for adjusting the height of the guide frame 672 is fixedly connected to the lower side of the housing 1. Six sets of fourth solenoid valves 673 are fixedly connected to the upper side of the guide frame 672 corresponding to the lower side of the six sets of screening shells 63. A second docking ring 674 that matches the first docking ring 664 is fixedly connected to the upper end of the fourth solenoid valve 673. A bellows 675 is fixedly connected to the bottom of the fourth solenoid valve 673. A discharge pipe 676 that is located at the bottom of the housing 1 and is used to discharge the phage solution is fixedly connected to the bottom of the bellows 675.

[0054] The second docking ring 674 has a docking groove on its upper side that matches the first docking ring 664 and a sealing element on its edge. The discharge pipe 676 is connected to an external material extraction device. The discharge end of the material extraction device is connected to an external low-temperature ultrafiltration concentration unit. The low-temperature ultrafiltration concentration unit is connected to an external purification device. The external purification device is connected to an external refrigerated storage cabinet. The extended end of the cylinder 671 is fixedly connected to the guide frame 672. After the angle of the array screening shell 63 is adjusted, ensure that the first docking ring 664 and the second docking ring 674 on the lower side of the screening shell 63 are aligned. Adjust the guide frame 672, the fourth solenoid valve 673, and the first solenoid valve 674 through the cylinder 671. During this process, the bellows 675 is stretched under the traction of the fourth solenoid valve 673, allowing the first docking ring 664 to engage in the docking groove within the second docking ring 674. When it is necessary to export the solution after low-temperature treatment, the corresponding third solenoid valve 661 and fourth solenoid valve 673 are opened, allowing the external extraction component to draw the solution through the third solenoid valve 661, three-way valve 662, discharge pipe 663, first docking ring 664, second docking ring 674, bellows 675, and discharge pipe 676, allowing the extraction component to inject the solution into the low-temperature ultrafiltration concentration unit for low-temperature filtration treatment.

[0055] Furthermore, any content not described in detail in this specification is existing technology known to those skilled in the art.

[0056] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0057] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for screening and preparing low-temperature resistant Vibrio phages, characterized in that: Specifically, the following steps are included: Step 1: Import the Vibrio phage sample solution to be screened into the screening container. After loading multiple sets of screening containers in batches, align four sets of screening containers with four independent temperature control areas, and complete the sealing connection between the screening containers and the drainage structure. Step 2: Compressed air is generated and injected into four sets of airflow generating structures after passing through a splitting structure to produce cold air and hot air. The cold air is introduced into four independent temperature-controlled zones through splitting pipes. The Vibrio phage solution in the four screening containers is independently regulated through heat exchange, so that the screening temperature in each screening container is maintained in a low temperature range of 0℃ to 12℃ without interference. At the same time, the Vibrio phage sample solution in the screening container is stirred to promote the full progress of the screening reaction. Step 3: After the screening operation is completed, the solutions in the four screening containers are extracted to the corresponding low-temperature ultrafiltration concentration units through the drainage structure for low-temperature concentration treatment. After concentration, the solutions are purified and finally the low-temperature resistant Vibrio phages obtained from the screening are stored.

2. The method for screening and preparing low-temperature resistant Vibrio phage according to claim 1, characterized in that: In step two, each airflow generating structure is equipped with a set of regulating components. The flow rate at the hot air emission end of the airflow generating structure is independently controlled by the regulating components, thereby regulating the temperature at the cold air emission end of the airflow generating structure and ensuring that the temperatures of the four independent temperature control zones do not interfere with each other.

3. A device for screening and preparing low-temperature resistant Vibrio phages, characterized in that: For implementing the method for screening and preparing low-temperature resistant Vibrio phage as described in any one of claims 1-2, the device includes a housing (1), the housing (1) being connected to a processing component for screening and preparing low-temperature resistant Vibrio phage, the processing component including: The feeding component (2) is installed on the upper side of the shell (1) for feeding low-temperature Vibrio phage samples; The array of support feet (4) is fixedly connected to the bottom edge of the housing (1) to provide support; Storage component (6), installed on housing (1) for storing and adjusting low-temperature resistant Vibrio phage samples exported by feeding component (2), the storage component (6) includes a rotating seat (61) rotatably connected to the inside of housing (1), six sets of screening shells (63) are fixedly connected to the upper edge of the rotating seat (61), a temperature sensor is fixedly connected to the rotating seat (61) and attached to the lower end of the screening shell (63), a set of heat preservation plates (62) are fixedly connected to the rotating seat (61) on both sides near the screening shell (63), a stirring component (64) for providing stirring for the samples in the six sets of screening shells (63) is installed on the lower side of the rotating seat (61), a driving component (65) for driving the rotating seat (61) to rotate is fixedly connected to the housing (1), a draining component (66) for draining liquid from the screening shell (63) is connected to the driving component (65), and a docking component (67) for assisting the draining component (66) in draining liquid is installed on the lower side of the housing (1). Adjustment component (5), installed on housing (1), is used to adjust the temperature of screening shell (63); The cleaning component (3) is installed on the upper rear end of the housing (1) for cleaning the screening shell (63).

4. The apparatus for screening and preparing low-temperature resistant Vibrio phages according to claim 3, characterized in that: The cleaning component (3) includes a water pump (31) fixedly connected to the housing (1). A spray plate (34) is fixedly connected to the upper side of the housing (1) near a set of screening shells (63). A conduit (32) is fixedly connected to the drain end of the water pump (31). A first solenoid valve (33) is fixedly connected to the upper side of the spray plate (34) and fixedly connected to the conduit (32). A pumping pipe is fixedly connected to the pumping end of the water pump (31).

5. The apparatus for screening and preparing low-temperature resistant Vibrio phages according to claim 3, characterized in that: The adjusting component (5) includes a first temperature-regulating ring (56) and a second temperature-regulating ring (57) fixedly connected to the top of the inner side of the housing (1). The outer wall of the first temperature-regulating ring (56) is slidably connected to the insulation plate (62) and the screening shell (63) respectively. The inner wall of the second temperature-regulating ring (57) is slidably connected to the insulation plate (62) and the screening shell (63) respectively. The first temperature-regulating ring (56) and the second temperature-regulating ring (57) are aligned with the axis of the rotating seat (61) and have four sets of non-communicating temperature-regulating cavities on their inner sides. Four sets of second branch pipes (55) and third branch pipes (58) are fixedly connected to the four sets of temperature regulating chambers. The second branch pipe (55) is fixedly connected to the second solenoid valve (54). The end of the second solenoid valve (54) away from the housing (1) is fixedly connected to the vortex tube (53) for generating cold air. The air inlet end of the four sets of vortex tubes (53) is fixedly connected to the first branch pipe (52). The front side of the first branch pipe (52) is fixedly connected to the air compressor (51). The side of the vortex tube (53) away from the second solenoid valve (54) is fixedly connected to the flow valve (59).

6. The apparatus for screening and preparing low-temperature resistant Vibrio phages according to claim 5, characterized in that: The first temperature regulating ring (56) and the second temperature regulating ring (57) are made of aluminum alloy near the ends of the four temperature regulating cavities. Four sets of heat insulation plates are installed on the inner side of the first temperature regulating ring (56) and the second temperature regulating ring (57) between the four temperature regulating cavities. The heat insulation plates are used to separate the four temperature regulating cavities from each other to ensure that the temperature of each temperature regulating cavity does not interfere with each other. The bottom of the first temperature regulating ring (56) and the second temperature regulating ring (57) are attached to the upper end of the rotating seat (61). The second branch pipe (55) and the third branch pipe (58) are both sealed and connected to the temperature regulating cavity.

7. The apparatus for screening and preparing low-temperature resistant Vibrio phages according to claim 3, characterized in that: The stirring component (64) includes six sets of stirring rods (644) that are rotatably connected to six sets of screening shells (63). The stirring rods (644) are coaxially fixedly connected to the lower end of the rotating seat (61) with a first gear (642). The six sets of first gears (642) mesh together with a set of first gear rings (643) that are rotatably connected to the lower edge of the rotating seat (61). The lower right end of the rotating seat (61) is fixedly connected to a first motor (641). The first motor (641) is coaxially fixedly connected to a set of first gears (642).

8. The apparatus for screening and preparing low-temperature resistant Vibrio phages according to claim 3, characterized in that: The drive unit (65) includes a support base (651) that is coaxially fixedly connected to the bottom of the rotating seat (61) and rotatably connected to the housing (1). A second gear ring (654) is coaxially fixedly connected to the lower side of the support base (651). A second motor (652) is fixedly connected to the lower left end of the housing (1). A second gear (653) that meshes with the second gear ring (654) is coaxially fixedly connected to the upper output end of the second motor (652). A fourth branch pipe (655) that is connected to the draining component (66) is fixedly connected to the middle part of the support base (651). A drain pipe (656) that is rotatably connected to and communicates with the bottom of the fourth branch pipe (655) is fixedly connected to the middle part of the bottom of the housing (1).

9. The apparatus for screening and preparing low-temperature resistant Vibrio phages according to claim 3, characterized in that: The drain component (66) includes a third solenoid valve (661) fixedly connected to the lower end of the screening shell (63). A three-way valve (662) for switching the liquid flow direction and connected to the drive component (65) is fixedly connected to the bottom of the third solenoid valve (661). A discharge pipe (663) is fixedly connected to the bottom of the three-way valve (662). A first docking ring (664) is fixedly connected to the bottom of the discharge pipe (663). The docking component (67) includes a guide frame (672) slidably connected to the bottom of the shell (1). A first docking ring (664) is fixedly connected to the lower side of the shell (1). A cylinder (671) is used to adjust the height of the guide frame (672). The upper side of the guide frame (672) is fixedly connected to the lower side of the six sets of screening shells (63). The upper end of the fourth solenoid valve (673) is fixedly connected to a second docking ring (674) that matches the first docking ring (664). The bottom of the fourth solenoid valve (673) is fixedly connected to a bellows (675). The bottom of the bellows (675) is fixedly connected to an outlet pipe (676) located at the bottom of the shell (1) for screening phage solution outflow.

10. The apparatus for screening and preparing low-temperature resistant Vibrio phages according to claim 9, characterized in that: The second docking ring (674) has a docking groove on its upper side that matches the first docking ring (664) and a sealing element on its edge. The discharge pipe (676) is connected to an external material extraction component. The extended end of the cylinder (671) is fixedly connected to the guide frame (672).

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