A snp molecular marker combination and identification method for wannan flower pig identification

By screening specific SNP sites of Wannan Spotted Pig and using the PLS-DA model, the problems of speed, accuracy and stability in the identification of Wannan Spotted Pig in existing technologies have been solved. This enables efficient identification of individual Wannan Spotted Pigs and their related samples, and is applicable to the traceability of breeds in live tissues, post-slaughter samples and deep-processed meat products.

CN122104933APending Publication Date: 2026-05-29ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ANHUI AGRICULTURAL UNIVERSITY
Filing Date
2026-02-12
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing technologies struggle to achieve rapid, accurate, and reproducible identification of Southern Anhui Spotted Pigs in a multi-breed context. Traditional molecular marker methods suffer from limitations such as a limited number of markers, complex interpretation of typing results, and low standardization. Furthermore, pedigree and phenotypic methods are susceptible to limitations imposed by record integrity and testing scenarios.

Method used

Eleven SNP loci specific to the Wannan spotted pig were screened using whole-genome resequencing. Combined with a partial least squares discriminant analysis (PLS-DA) model, a stable identification method was established through numerical coding and standardization, and a detection kit was prepared for testing.

Benefits of technology

It enables rapid, accurate, and repeatable identification of individual Anhui Southern Flower Pigs and their related meat products, reducing testing costs and operational complexity, and improving the reliability and traceability of identification results. It is applicable to the traceability of live tissues, post-slaughter samples, and deep-processed meat products.

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Abstract

The present application relates to pig breed molecular identification, and discloses a SNP molecular marker combination for Wannan flower pig identification, the combination is composed of the following 11 SNP sites, the physical position and allele information of each SNP site is determined based on pig reference genome Sscrofa11.1: rs342792776, located at 103395244bp of chromosome 4, the allele thereof is A / G; rs343665183, located at 104083270bp of chromosome 4, the allele thereof is G / A; novel_WNS1, located at 5240118bp of chromosome 7, the allele thereof is G / A.The present application is based on whole genome resequencing and population genetics analysis, 11 Wannan flower pig specific SNP sites are screened out, and unified REF / ALT unification, 0 / 1 / 2 numerical coding, standardization processing and threshold determination rules are combined, so that the detection process is clear, the repeatability is strong, and the standardized implementation between different detection platforms and laboratories is facilitated.
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