HBV PreS1 antibody qualitative detection test strip, its preparation method and application

The HBV PreS1 antibody qualitative test strip was prepared using colloidal gold immunochromatography technology, which solves the problems of complexity and equipment dependence of existing detection methods, and realizes rapid and convenient HBV PreS1 antibody detection, which is suitable for primary healthcare institutions and home self-testing.

CN122109528BActive Publication Date: 2026-07-03JILIN UNIVERSITY +2
View PDF 2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JILIN UNIVERSITY
Filing Date
2026-04-10
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Existing HBV PreS1 antibody detection methods are complex to operate, time-consuming, and highly dependent on equipment, making it difficult to meet the needs of primary healthcare institutions and on-site testing for speed and convenience.

Method used

Using colloidal gold immunochromatography, PreS1 antigen is used as the detection line. By specifically binding PreS1 antigen to PreS1 antibody in the sample, a qualitative test strip for HBV PreS1 antibody is prepared, simplifying the detection process and enabling rapid and convenient detection.

Benefits of technology

The test can be completed in 15 minutes, requires no complicated equipment, and is suitable for primary healthcare institutions and home self-testing. It provides a fast and convenient tool for assessing HBV immune status and is suitable for viral biomarker detection and clinical point-of-care testing.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN122109528B_ABST
    Figure CN122109528B_ABST
Patent Text Reader

Abstract

This invention discloses a qualitative test strip for HBV PreS1 antibody, its preparation method, and its applications, relating to the fields of virology, immunology, biodetection technology, and immunochromatographic analysis. Based on the principle of antigen-antibody specific recognition, the test strip achieves rapid detection of PreS1 antibodies in serum or plasma samples through the specific binding of PreS1 antigen and PreS1 antibody. It is assembled sequentially from a sample pad, a gold-labeled pad, a nitrocellulose membrane (NC membrane), and an absorbent pad. The gold-labeled pad is immobilized with colloidal gold-labeled anti-human IgG antibody as a gold probe. The NC membrane detection line (T line) is coated with HBV PreS1 antigen to capture PreS1 antibodies in the sample, and the NC membrane control line (C line) is coated with HB2 antibody to determine the effectiveness of the test strip's detection process. The test strip of this invention requires no complex instruments or equipment, the detection process is simple, rapid, and has good sensitivity and specificity, making it suitable for evaluating the immunization efficacy of hepatitis B vaccines and for large-scale screening.
Need to check novelty before this filing date? Find Prior Art