An anti-aging composition synergistically enhancing mitochondrial function by AKG-pqq

Abstract

This invention discloses an anti-aging composition that synergistically enhances mitochondrial function using AKG-PQQ, relating to the field of anti-aging technology. The composition comprises AKG, disodium PQQ, button mushroom extract, and excipients. By combining AKG, disodium PQQ, button mushroom extract, and a single excipient, AKG, as 2-oxoglutarate, directly participates in mitochondrial energy metabolism in anhydrous crystalline form, providing core substrate support for mitochondrial function. Disodium PQQ promotes mitochondrial regeneration, ensuring mitochondrial structural renewal and physiological activity. The naturally occurring β-glucan and ergothioneine in the button mushroom extract form a synergistic effect, maintaining the normal physiological state of mitochondria from multiple dimensions. The single excipient only physically mixes with the active ingredients, avoiding interference from interactions between multiple excipients and ensuring the full effectiveness of each active ingredient.

Description

Technical Field

[0001] This invention relates to the field of anti-aging technology, specifically to an anti-aging composition that synergistically enhances mitochondrial function using AKG-PQQ. Background Technology

[0002] Aging is a natural decline in physiological functions that occurs over time. Mitochondrial dysfunction is an important cause of aging. As the core of cellular energy metabolism, the functional state of mitochondria directly affects the normal physiological activities of cells, and thus relates to the overall aging process of the body. With the increasing demand for health, regulating mitochondrial function and delaying aging through functional compositions has become a current research hotspot. Anti-aging compositions with natural active ingredients have gained widespread attention due to their safety advantages.

[0003] However, most anti-aging products on the market today have a single mechanism of action, making it difficult to synergistically intervene in the aging process from multiple dimensions such as energy metabolism, mitochondrial biogeneration, and antioxidation. They do not incorporate substances containing natural active ingredients, such as button mushroom extract, and therefore cannot form a multi-dimensional mitochondrial function regulation system. Furthermore, the excipients used in anti-aging compositions are often in multiple compound forms, which can easily lead to interactions between excipients and may interfere with the effects of active ingredients. At the same time, the preparation of the compositions lacks standardized control, and the extraction and granulation operations are rough, which can easily lead to the loss of active ingredients and make it difficult to ensure the stable effect of the composition. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of existing technologies and provide an anti-aging composition that synergistically enhances mitochondrial function using AKG-PQQ. This invention combines AKG, disodium PQQ, button mushroom extract, and a single excipient. AKG, as 2-oxoglutarate, directly participates in the energy metabolism process of mitochondria in anhydrous crystalline form, providing core substrate support for mitochondrial function. Disodium PQQ promotes mitochondrial regeneration, ensuring mitochondrial structural renewal and physiological activity. The naturally occurring β-glucan and ergothioneine in button mushroom extract form a synergistic effect, maintaining the normal physiological state of mitochondria from multiple dimensions. The single excipient only physically mixes with the active ingredient, avoiding interference from interactions between multiple excipients and ensuring that the effects of each active ingredient are fully realized.

[0005] To solve the above-mentioned technical problems, the present invention provides the following technical solution: In one aspect, an anti-aging composition for AKG-PQQ synergistically enhancing mitochondrial function, wherein the composition, by weight, comprises: AKG: 40-65 servings; PQQ disodium salt: 0.05-0.5 parts; Button mushroom extract: 15-35 parts; Additional ingredients: 10-20 portions.

[0006] Furthermore, the AKG is 2-oxoglutaric acid, which is in anhydrous crystalline form with a water solubility of not less than 100 g / L. It is used directly as an active ingredient without any chemical modification.

[0007] Furthermore, the PQQ disodium salt is pyrroloquinoline quinone disodium salt, which is a white to pale yellow crystalline powder, easily soluble in water, and has a residual solvent content of <0.001%.

[0008] Furthermore, the button mushroom extract is an extract obtained from button mushrooms through a water extraction and alcohol precipitation process. The raw material for extraction is fresh or dried button mushroom fruiting bodies, which naturally contain β-glucan and ergothioneine. The button mushroom extract contains 10-30% β-glucan by mass and 0.001-0.01% ergothioneine by mass. The drying weight loss rate of the button mushroom extract is ≤5%. The extract is a light yellow to brownish-yellow powder with a water-soluble extract content ≥40%.

[0009] Furthermore, the excipients are one of mannitol, lactose, microcrystalline cellulose, and magnesium stearate. The excipients are food-grade raw materials with no industrial-grade raw materials added. Moreover, the excipients only undergo physical mixing with AKG, PQQ disodium salt, and button mushroom extract, without any chemical cross-linking reaction.

[0010] On the other hand, an anti-aging composition that synergistically enhances mitochondrial function using AKG-PQQ is prepared as follows: Raw material pretreatment: The fruiting bodies of Agaricus bisporus were subjected to water extraction and alcohol precipitation pretreatment to obtain Agaricus bisporus extract. Then, AKG, PQQ disodium salt, Agaricus bisporus extract and excipients were subjected to impurity removal and grinding pretreatment respectively. Ingredient mixing: The pretreated AKG, PQQ disodium salt, button mushroom extract and excipients are added to the mixing equipment according to the ratio and stirred to obtain a homogeneous mixture. Material forming: The mixture is formed by dry granulation process to obtain the initial material; Drying process: The initial material is placed in a drying device to remove free moisture. The dried material is then processed to remove any clumps, resulting in the final product composition.

[0011] Furthermore, in the raw material pretreatment, the water extraction and alcohol precipitation pretreatment process of the button mushroom fruiting bodies is as follows: the button mushroom fruiting bodies are crushed and extracted with water, then ethanol is added to adjust the alcohol concentration of the system, and after standing, the precipitate is collected by centrifugation and dried; wherein the material-to-liquid ratio of water extraction is 1:8-15, the extraction temperature is 70-90℃, and the extraction time is 1-3h; the alcohol concentration of the alcohol precipitation system is 60-80%, the standing temperature is 4-10℃, the standing time is 8-16h, the centrifugation speed is 3000-5000r / min, and the centrifugation time is 10-20min; impurities are removed by vibrating screen with a vibration frequency of 20-30Hz; the grinding time is 5-15min, and the ground material is sieved to a mesh size of 80-120 mesh to remove coarse particles that do not pass through the sieve, leaving only fine powdery homogeneous material.

[0012] Furthermore, in the mixing of the components, the ratio of AKG, PQQ disodium salt, button mushroom extract and excipients is 40-65:0.05-0.5:15-35:10-20; the stirring speed of the mixing equipment is 100-300 r / min, the ambient temperature during the mixing process is 20-25℃, the relative humidity is 40-60%, and the mixing is continued until the materials are uniformly mixed.

[0013] Furthermore, in the material forming process, the dry granulation process is as follows: the mixed material is pre-granulated using a 16-20 mesh sieve, and then fed into a dry granulator for tableting. The tableting roller linear speed is 5-15 m / min, and the granulation pressure is 5-15 MPa, resulting in thin sheet material. The thin sheet material is then pulverized in the dry granulator and passed through a 20-30 mesh sieve. The sieved material undergoes initial granulation, and after initial granulation, it is passed through an 8-30 mesh sieve to complete the dry granulation process and obtain pre-formed granular material.

[0014] Furthermore, in the drying process, the drying temperature is 40-60℃, the drying time is 2-6 hours, and the moisture content after drying is 1-3%.

[0015] Compared with existing technologies, this AKG-PQQ synergistic anti-aging composition for enhancing mitochondrial function has the following beneficial effects: I. This invention combines AKG, disodium PQQ, and button mushroom extract with a single excipient. AKG, as 2-oxoglutarate, directly participates in the energy metabolism process of mitochondria in anhydrous crystalline form, providing core substrate support for mitochondrial function. Disodium PQQ promotes mitochondrial regeneration, ensuring mitochondrial structural renewal and physiological activity. The naturally occurring β-glucan and ergothioneine in button mushroom extract form a synergistic effect, maintaining the normal physiological state of mitochondria from multiple dimensions. The single excipient only physically mixes with the active ingredient, avoiding the interference of interactions between multiple excipients on the function of the active ingredient, and ensuring that the effects of each active ingredient are fully exerted.

[0016] II. This invention prepares an extract from the fruiting bodies of Agaricus bisporus using a water extraction and alcohol precipitation process, fully preserving the natural active ingredients of β-glucan and ergothioneine in the extract. AKG, PQQ disodium salt, Agaricus bisporus extract, and single excipients are then subjected to impurity removal and grinding pretreatment to ensure the uniformity of the fine powder. A dry granulation physical molding process is used to form the material, avoiding the damage to active ingredients caused by chemical modification. Drying removes free moisture from the material, ensuring the stability of the composition. This provides comprehensive protection for each active ingredient, ensuring the role of each component in regulating mitochondrial function and allowing the anti-aging effect of the composition to be stably manifested.

[0017] Other advantages, objectives and features of the invention will be set forth in part in the description which follows, and in part will be apparent to those skilled in the art from the following examination or study, or may be learned from the practice of the invention. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0019] Figure 1 A flowchart illustrating a method for preparing an anti-aging composition that synergistically enhances mitochondrial function using AKG-PQQ; Figure 2 A framework diagram of a method for preparing an anti-aging composition that synergistically enhances mitochondrial function using AKG-PQQ; Figure 3 This is a framework diagram of the raw material pretreatment process in the preparation method of an anti-aging composition that synergistically enhances mitochondrial function using AKG-PQQ. Detailed Implementation

[0020] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with the accompanying drawings and preferred embodiments, is provided below.

[0021] Example 1: Raw material preparation (by weight): AKG 50 copies; 0.2 parts of PQQ disodium salt; 29.8 parts of button mushroom extract; 20 portions of auxiliary materials.

[0022] Raw material specifications: AKG: 2-Oxoglutaric acid, in anhydrous crystalline form, with a water solubility of 110 g / L. It is used directly as an active ingredient without chemical modification. PQQ disodium salt: is the disodium salt of pyrroloquinoline quinone, a white crystalline powder, easily soluble in water, with a residual solvent content of <0.001%; Button mushroom extract: It is obtained from dried button mushroom fruiting bodies through water extraction and alcohol precipitation. It is a light yellow powder with a β-glucan content of 20% by mass, an ergothioneine content of 0.005% by mass, a drying loss rate of 3%, and a water-soluble extract content of 45%. Excipient: Mannitol, a food-grade raw material with no industrial-grade raw materials added. It only undergoes physical mixing with the active ingredient without any chemical cross-linking reaction.

[0023] Preparation method: The dried fruiting bodies of *Agaricus bisporus* were pulverized and extracted with water at a material-to-liquid ratio of 1:10 at 80℃ for 2 hours. Ethanol was added to the filtrate to adjust the alcohol concentration to 70%, and the mixture was allowed to stand at 5℃ for 12 hours. After centrifugation at 4000 rpm for 15 minutes, the precipitate was collected and dried to obtain *Agaricus bisporus* extract. AKG, PQQ disodium salt, *Agaricus bisporus* extract, and mannitol were separately purified using a vibrating sieve at a vibration frequency of 25 Hz. The mixture was then ground for 10 minutes using a high-speed universal pulverizer. The ground material was then passed through a 100-mesh sieve to remove coarse particles, leaving a fine, homogeneous powder. Figure 1 As shown.

[0024] Pretreated AKG, PQQ disodium salt, Agaricus bisporus extract, and mannitol were added to a three-dimensional motion mixer at a weight ratio of 50:0.2:29.8:20. The mixing speed was 200 r / min, the ambient temperature was 23℃, and the relative humidity was 50%. The mixture was stirred until it was homogeneous, resulting in a mixture. Figure 2 As shown.

[0025] The mixture is pre-granulated using an 18-mesh sieve. The pre-granulated material is then fed into a dry granulator for tableting. The tableting roller linear speed is 10 m / min, and the granulation pressure is 10 MPa, resulting in thin flakes. The thin flakes are then pulverized in the dry granulator and passed through a 25-mesh sieve. The sieved material undergoes initial granulation and is then passed through a 20-mesh sieve to complete the dry granulation process, yielding pre-formed granules.

[0026] The pre-formed granular material was placed in a hot air circulating drying oven and dried at 50°C for 4 hours. The moisture content of the dried material was 2%. Subsequently, the dried material was sieved to remove agglomerated material, and the finished product AKG-PQQ anti-aging composition that synergistically enhances mitochondrial function was obtained.

[0027] Physicochemical index testing: The finished product consists of uniform light yellow granules with a particle size ranging from 8 to 30 mesh, free from lumps and discoloration. The active ingredients and mannitol are only physically mixed without any chemical cross-linking reaction. The retention rates of active ingredients in AKG, PQQ disodium salt, and button mushroom extract are 98.6%, 98.9%, and 98.4%, respectively.

[0028] Functionality and performance testing: In vitro cultured aging model hepatocytes were cultured with the anti-aging composition of this embodiment for 48 hours, and the mitochondrial membrane potential was detected. The results showed that the fluorescence intensity of the mitochondrial membrane potential of hepatocytes was at a high level, indicating that AKG provides sufficient substrate support for mitochondrial function, PQQ disodium salt helps maintain mitochondrial structure, and β-glucan and ergothionein in Agaricus bisporus extract act on the mitochondrial membrane. The three work together to stabilize the potential difference across the mitochondrial membrane, effectively maintain the stability of the mitochondrial membrane potential, and ensure the electrochemical transmission function of mitochondria.

[0029] In vitro cultured aging model hepatocytes were cultured with the anti-aging composition of this embodiment for 48 hours, and the level of mitochondrial reactive oxygen species (ROS) was detected. The results showed that the ROS content in mitochondria was significantly reduced, indicating that ergothionein in Agaricus bisporus extract exerts an antioxidant effect, and AKG reduces the generation of reactive oxygen species during mitochondrial metabolism. The two, together with disodium PQQ, form a synergistic effect. Combined with the physical dispersion properties of mannitol, the active ingredients can act evenly on the mitochondria, reduce the interference of oxidative damage on mitochondrial function, and enhance the mitochondrial oxidative stress resistance.

[0030] Example 2: Raw material preparation (by weight): AKG 60 copies; 0.4 parts of PQQ disodium salt; 19.6 parts of button mushroom extract; 20 portions of auxiliary materials.

[0031] Raw material specifications: AKG: 2-Oxoglutaric acid, anhydrous crystalline form, water solubility is 105 g / L, untreated and used directly as an active ingredient; PQQ disodium salt: is the disodium salt of pyrroloquinoline quinone, a pale yellow crystalline powder, easily soluble in water, with a residual solvent content of <0.001%; Button mushroom extract: It is obtained from fresh button mushroom fruiting bodies through water extraction and alcohol precipitation. It is a brown powder with a β-glucan content of 28% by mass, an ergothioneine content of 0.008% by mass, a drying loss rate of 2.5%, and a water-soluble extract content of 48%. Excipient: Lactose, a food-grade raw material with no industrial-grade raw materials added. It only undergoes physical mixing with the active ingredients without any chemical cross-linking reaction.

[0032] Preparation method: The preparation process of the button mushroom extract, the removal of impurities from AKG, disodium PQQ salt, and lactose, as well as the grinding and sieving processes, are all the same as in Example 1. Figure 3 As shown; The pretreated raw materials were added to a three-dimensional motion mixer in a weight ratio of 60:0.4:19.6:20. The stirring speed and mixing environment parameters were the same as in Example 1. The mixture was stirred until the materials were uniformly mixed to obtain a mixture. The operation steps and parameters for pre-granulation, tableting, crushing, sieving and primary granulation are the same as in Example 1, and the initial granulated material is obtained. The drying equipment, temperature, time, and sieving operation were the same as in Example 1, resulting in the finished anti-aging composition AKG-PQQ that synergistically enhances mitochondrial function.

[0033] Physicochemical index testing: The finished product consists of uniform brown granules with a particle size ranging from 8 to 30 mesh, free from lumps and discoloration. The active ingredients and lactose are only physically mixed without any chemical cross-linking reaction. The retention rates of active ingredients in AKG, PQQ disodium salt, and button mushroom extract are 98.7%, 99.1%, and 98.5%, respectively.

[0034] Functionality and performance testing: In vitro cultured aging model hepatocytes were cultured with the anti-aging composition of this embodiment for 48 hours, and the mitochondrial membrane potential was detected. The results showed that the fluorescence intensity of the mitochondrial membrane potential of hepatocytes was at a high level, indicating that the high content of AKG continuously provides sufficient metabolic substrates for mitochondria, the high ratio of PQQ disodium salt effectively promotes mitochondrial regeneration, strengthens the construction and repair of mitochondrial membrane structure, and the active ingredients in the button mushroom extract help maintain the integrity of mitochondrial membrane. The three work synergistically to effectively stabilize the mitochondrial membrane potential and ensure the normal electrochemical transport and energy conversion functions of mitochondria.

[0035] In vitro cultured aging model hepatocytes were cultured with the anti-aging composition of this embodiment for 48 hours, and the level of mitochondrial reactive oxygen species (ROS) was detected. The results showed that the ROS content in mitochondria was significantly reduced, indicating that the high content of AKG greatly reduced the generation of reactive oxygen species in mitochondrial metabolism. Disodium PQQ synergistically alleviated mitochondrial oxidative damage. Ergothioneine and β-glucan in Agaricus bisporus extract exerted antioxidant effects. The three components, combined with the mild dispersing properties of lactose, enabled the active ingredients to act evenly on mitochondria, significantly reducing the impact of oxidative damage on mitochondrial function and enhancing the mitochondrial resistance to oxidative stress.

[0036] Example 3: Raw material preparation (by weight): AKG 45 copies; 0.5 parts of PQQ disodium salt; 34.5 parts of button mushroom extract; 20 portions of auxiliary materials.

[0037] Raw material specifications: AKG: 2-Oxoglutaric acid, in anhydrous crystalline form, with a water solubility of 108 g / L. It is used directly as an active ingredient without any chemical modification. PQQ disodium salt: is the disodium salt of pyrroloquinoline quinone, a pale yellow crystalline powder, easily soluble in water, with a residual solvent content of <0.001%; Button mushroom extract: Obtained from dried button mushroom fruiting bodies through water extraction and alcohol precipitation process. It is a brownish-yellow powder, containing 25% β-glucan by mass, 0.009% ergothioneine by mass, 4% loss on drying, and 46% water-soluble extract. Excipient: Microcrystalline cellulose, a food-grade raw material with no industrial-grade raw materials added. It only undergoes physical mixing with the active ingredients without any chemical cross-linking reaction.

[0038] Preparation method: The preparation process of button mushroom extract, the removal of impurities, grinding and sieving of AKG, PQQ disodium salt and microcrystalline cellulose are the same as in Example 1; The pretreated raw materials were added to a three-dimensional motion mixer in a weight ratio of 45:0.5:34.5:20. The stirring speed and mixing environment parameters were the same as in Example 1. The mixture was stirred until it was homogeneous to obtain a mixture. The operation steps and parameters for pre-granulation, tableting, crushing, sieving and primary granulation are the same as in Example 1, and the initial granulated material is obtained. The drying equipment, temperature, time, and subsequent sieving operations were the same as in Example 1, resulting in the finished AKG-PQQ anti-aging composition that synergistically enhances mitochondrial function.

[0039] Physicochemical index testing: The finished product consists of uniform brownish-yellow granules with a particle size ranging from 8 to 30 mesh, free from lumps and discoloration. The active ingredients and microcrystalline cellulose are only physically mixed without any chemical cross-linking reaction. The retention rates of active ingredients in AKG, PQQ disodium salt, and button mushroom extract are 98.9%, 99.2%, and 98.8%, respectively.

[0040] Functionality and performance testing: In vitro cultured aging model hepatocytes were cultured with the anti-aging composition of this embodiment for 48 hours, and the mitochondrial membrane potential was measured. The results showed that the fluorescence intensity of the mitochondrial membrane potential of hepatocytes was at a high level, indicating that β-glucan and ergothionein in the high-ratio Agaricus bisporus extract effectively maintain the integrity of the mitochondrial membrane, PQQ disodium salt promotes mitochondrial regeneration in a high ratio, and AKG continuously supplies metabolic substrates for mitochondrial function. The three work synergistically to stabilize the potential difference across the mitochondrial membrane, efficiently maintain the stability of the mitochondrial membrane potential, and ensure the electrochemical transmission function of mitochondria.

[0041] In vitro cultured aging model hepatocytes were cultured with the anti-aging composition of this embodiment for 48 hours, and the level of mitochondrial reactive oxygen species (ROS) was detected. The results showed that the ROS content in mitochondria was significantly reduced. The high ratio of button mushroom extract brought abundant ergothioneine and β-glucan, which exerted a strong antioxidant effect. AKG reduced the generation of reactive oxygen species during mitochondrial metabolism, and PQQ disodium salt synergistically alleviated mitochondrial oxidative damage. The combination of these three with the good shaping and dispersion characteristics of microcrystalline cellulose allowed the active ingredients to act evenly on the mitochondria, greatly reducing the interference of oxidative damage on mitochondrial function and significantly improving the mitochondrial oxidative stress resistance.

[0042] Comparative example: The core component of button mushroom extract is missing, and the excipients are in multiple compound forms. The rest of the preparation process is the same as in Examples 1, 2 and 3.

[0043] Raw material preparation (by weight): AKG 52 copies; 0.3 parts of PQQ disodium salt; 17.7 parts of auxiliary materials.

[0044] Raw material specifications: The excipients are 8 parts food-grade mannitol, 6 parts food-grade lactose, and 3.7 parts food-grade magnesium stearate. The raw material specifications of AKG, PQQ disodium salt, and excipients are consistent with the corresponding raw materials in Examples 1, 2, and 3. They are all food-grade, and no industrial-grade raw materials are added.

[0045] Preparation method: The process steps and parameters of raw material pretreatment, component mixing, material forming, and drying are completely consistent with those of Examples 1, 2, and 3.

[0046] Physicochemical index testing: The finished product is a pale yellow, uneven granule, with about 30% of the particles exceeding the 8-30 mesh limit. There is obvious slight clumping and local discoloration. Because the excipients are in multiple compound forms, there is a non-specific physical adsorption between them and AKG and PQQ disodium salts, which is not a simple physical mixing state. The retention rates of active ingredients of AKG and PQQ disodium salts are 92.2% and 92.8%, respectively, and the moisture content of the dried material is 2.5%.

[0047] Functionality and performance testing: The same in vitro cultured aging model hepatocytes as in Examples 1, 2, and 3 were selected. Under the same culture and detection conditions, the comparative anti-aging composition was added and cultured for 48 hours before the mitochondrial membrane potential was measured. The results showed that the fluorescence intensity of the mitochondrial membrane potential of hepatocytes was significantly lower, only 65.2% of that in Example 1, 58.7% of that in Example 2, and 52.3% of that in Example 3. Due to the absence of the core membrane protection component, Agaricus bisporus extract, the substrate supply from AKG and the mitochondrial regeneration effect of PQQ disodium salt could not form a multi-dimensional synergistic regulatory system, making it difficult to stabilize the potential difference across the mitochondrial membrane, and the mitochondrial membrane potential maintenance effect was greatly reduced.

[0048] Under the same conditions as in Examples 1, 2, and 3, the level of reactive oxygen species (ROS) in hepatocytes was measured. The results showed that the ROS content in mitochondria was significantly increased, being 2.1 times that of Example 1, 2.3 times that of Example 2, and 2.5 times that of Example 3. Due to the lack of antioxidant effects of ergothioneine and β-glucan in the Agaricus bisporus extract, and the physical adsorption effect caused by the compounding of multiple excipients interfering with the normal function of AKG and PQQ disodium salt, it was impossible to effectively reduce the generation of reactive oxygen species in mitochondrial metabolism, nor could it alleviate the effects of oxidative damage on mitochondria. The mitochondria had extremely poor resistance to oxidative stress.

[0049] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. An anti-aging composition that synergistically enhances mitochondrial function using AKG-PQQ, characterized in that, The composition, by weight, comprises: AKG: 40-65 servings; PQQ disodium salt: 0.05-0.5 parts; Button mushroom extract: 15-35 parts; Additional ingredients: 10-20 portions.

2. The anti-aging composition for synergistic enhancement of mitochondrial function by AKG-PQQ according to claim 1, characterized in that, The AKG is 2-oxoglutaric acid, in anhydrous crystalline form, with a water solubility of not less than 100 g / L, and has not undergone chemical modification.

3. The anti-aging composition for synergistic enhancement of mitochondrial function by AKG-PQQ according to claim 1, characterized in that, The PQQ disodium salt is pyrroloquinoline quinone disodium salt, which is a white to pale yellow crystalline powder, easily soluble in water, and has a residual solvent content of <0.001%.

4. The anti-aging composition for synergistic enhancement of mitochondrial function by AKG-PQQ according to claim 1, characterized in that, The button mushroom extract is an extract obtained from button mushrooms through a water extraction and alcohol precipitation process. The raw material for extraction is fresh or dried button mushroom fruiting bodies, which naturally contain β-glucan and ergothioneine. The button mushroom extract contains 10-30% β-glucan by mass and 0.001-0.01% ergothioneine by mass. The drying weight loss rate of the button mushroom extract is ≤5%. The extract is a light yellow to brownish-yellow powder with a water-soluble extract content ≥40%.

5. The anti-aging composition for synergistic enhancement of mitochondrial function by AKG-PQQ according to claim 1, characterized in that, The excipient is one of mannitol, lactose, microcrystalline cellulose, and magnesium stearate, and the excipient is a food-grade raw material.

6. An anti-aging composition that synergistically enhances mitochondrial function using AKG-PQQ, characterized in that, The preparation method of this composition is as follows: Raw material pretreatment: The fruiting bodies of Agaricus bisporus were subjected to water extraction and alcohol precipitation pretreatment to obtain Agaricus bisporus extract. Then, AKG, PQQ disodium salt, Agaricus bisporus extract and excipients were subjected to impurity removal and grinding pretreatment respectively. Ingredient mixing: The pretreated AKG, PQQ disodium salt, button mushroom extract and excipients are added to the mixing equipment according to the ratio and stirred to obtain a homogeneous mixture. Material forming: The mixture is formed by dry granulation process to obtain the initial shaped material; Drying process: The initial material is placed in a drying device to remove free moisture. The dried material is then processed to remove any clumps, resulting in the final product composition.

7. The anti-aging composition for synergistic enhancement of mitochondrial function by AKG-PQQ according to claim 6, characterized in that, In the raw material pretreatment, the water extraction and alcohol precipitation pretreatment process of Agaricus bisporus fruiting bodies is as follows: Agaricus bisporus fruiting bodies are crushed and extracted with water, then ethanol is added to adjust the alcohol concentration of the system, and after standing, the precipitate is collected by centrifugation and dried; wherein the material-to-liquid ratio of water extraction is 1:8-15, the extraction temperature is 70-90℃, and the extraction time is 1-3h; the alcohol concentration of the system for alcohol precipitation is 60-80%, the standing temperature is 4-10℃, the standing time is 8-16h, the centrifugation speed is 3000-5000r / min, and the centrifugation time is 10-20min; impurities are removed by vibrating screen with a vibration frequency of 20-30Hz; the grinding time is 5-15min, and the ground material is sieved to a mesh size of 80-120 mesh to remove coarse particles that do not pass through the sieve, leaving only fine powdery homogeneous material.

8. The anti-aging composition for synergistic enhancement of mitochondrial function by AKG-PQQ according to claim 6, characterized in that, In the mixing of the components, the ratio of AKG, PQQ disodium salt, button mushroom extract and excipients is 40-65:0.05-0.5:15-35:10-20; the stirring speed of the mixing equipment is 100-300 r / min, the ambient temperature during the mixing process is 20-25℃, the relative humidity is 40-60%, and the mixture is stirred until the materials are uniformly mixed.

9. The anti-aging composition for synergistic enhancement of mitochondrial function by AKG-PQQ according to claim 6, characterized in that, In the material forming process, the dry granulation process is as follows: the mixed material is pre-granulated using a 16-20 mesh sieve, and then fed into a dry granulator for tableting. The tableting roller linear speed is 5-15 m / min, and the granulation pressure is 5-15 MPa, resulting in thin sheet material. The thin sheet material is then pulverized in the dry granulator and passed through a 20-30 mesh sieve. The sieved material undergoes initial granulation, and after initial granulation, it is passed through an 8-30 mesh sieve to complete the dry granulation process and obtain the initially formed granular material.

10. The anti-aging composition for synergistic enhancement of mitochondrial function by AKG-PQQ according to claim 6, characterized in that, In the drying process, the drying temperature is 40-60℃, the drying time is 2-6 hours, and the moisture content after drying is 1-3%.

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