Method for improving survival rate of cuttage of adenos amurensis

Abstract

The application discloses a method for improving the cutting survival rate of Adenium obesum, which is composed of steps of cutting selection, cutting time, cutting method, preparation before cutting, cutting and post-stage management, and effectively solves the problems of low rooting rate and uneven growth of traditional cutting, so that the survival rate reaches more than 70%, the method is simple, convenient to operate and low in cost, and is suitable for practical production application. The method can reduce the digging of wild Adenium obesum, provide good plant materials for the fields of landscape engineering and family gardening, and provide stable, rich medicinal resources and technical support for the Chinese medicine industry.

Description

Technical Field

[0001] This invention relates to the field of planting technology, specifically to a method for improving the survival rate of Clematis grandiflora cuttings. Background Technology

[0002] Clematis patens, belonging to the genus Clematis in the family Ranunculaceae, is a high-quality vine with both ornamental and ecological value. It boasts rich flower colors, beautiful flower shapes, and strong resilience, making it particularly suitable for landscaping needs in cold northern regions. It has broad application prospects in vertical greening, courtyard landscaping, and floral design. However, this species exhibits significant seed dormancy in its natural state, with a germination rate of less than 10%. Traditional cutting propagation techniques suffer from problems such as unreasonable substrate ratios, indiscriminate use of hormones, and a lack of scientific basis for cutting selection, resulting in low rooting rates, unstable survival rates, and inconsistent seedling quality, making it difficult to meet the demands of large-scale production and market application. Currently, the yield of high-quality Clematis patens seedlings is low, leading to high costs and poor adaptability, further restricting its promotion and application in local landscaping. Therefore, developing an efficient, stable, and replicable Clematis patens cutting propagation technique to break through existing propagation bottlenecks and achieve large-scale cultivation of high-quality seedlings is of significant practical importance and application value for protecting wild resources, filling market gaps, and promoting the upgrading of the horticultural flower industry. By selecting appropriate cuttings, choosing the right time for cutting and inserting them, choosing the right cutting method, preparing before cutting, inserting the cuttings, and managing them afterward, the rooting rate of cuttings can be significantly improved. The method is simple, easy to operate, and has a low cost, making it suitable for practical production applications.

[0003] Upon searching, we found CN102177795A, which discloses "a method for propagating Clematis florida by cuttings." However, this method uses high-concentration hormone treatment, which is costly and may pose risks of contamination and phytotoxicity. Other reports on Clematis florida cuttings also have problems such as low survival rate, unstable results, and excessively long processing time. Summary of the Invention

[0004] The purpose of this invention is to provide a method for improving the survival rate of Clematis grandiflora cuttings. This technology aims to solve the problems of difficult seed germination and low cutting survival rate when artificially cultivating Clematis grandiflora.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0006] A method to improve the survival rate of Clematis grandiflora cuttings, the specific steps are as follows:

[0007] A method for improving the survival rate of Clematis grandiflora cuttings, characterized by the following specific steps:

[0008] 1. Selection of cuttings: Select healthy, disease-free branches from the top of the mother plant with plump and well-developed buds as scions;

[0009] 2. Timing of cutting and propagation: Cuttings should be taken in May during the spring, with the cutting and propagation occurring simultaneously.

[0010] 3. Cutting method: Discard the terminal bud and make small cuttings of 6-8 cm. Cut parallel to the first bud about 1 cm above it. Make a 45° angled cut at the bottom. Keep 2 small leaves on each cutting. After cutting, bundle 32 cuttings together and put them in a bottle with clean water for storage.

[0011] 4. Preparation before cuttings: Sterilize the substrate and then place it in a ventilated place to dry. One day before cuttings, mix the substrate according to the ratio and add carbendazim powder. After mixing thoroughly, put it into the seedling pot and water it thoroughly.

[0012] 5. Cuttings: Make a guide hole about 3 cm deep in the substrate in advance. Take the cutting out of the water, place it at an angle with the bottom down, and soak it in carbendazim for 10 minutes. After taking it out and washing it, use the straight insertion method to put the cutting into the small hole in the substrate, press it gently to compact the substrate, and water it thoroughly.

[0013] 6. Post-propagation management: After cutting, place the seedling pots indoors on the floor where there is no direct sunlight. Cover each seedling pot with a shade net with 50% light transmittance. Spray water with a sprayer every day, and the amount of water can be adjusted according to the degree of leaf wilting. At the same time, water the substrate to ensure that the humidity of the Clematis florida cuttings is about 90%. Spray more water in the first few days, and reduce the amount of water appropriately after the cuttings have stabilized to prevent the cuttings from rotting. After 10 days, move the seedling pots to a place with light, but still cover them with the shade net. Clean up dead branches and leaves in time every day, and spray with 0.3% carbendazim solution for disinfection every 10 days.

[0014] Furthermore, the matrix ratio is perlite:vermiculite:sand = 1:1:1.

[0015] Furthermore, the concentration of carbendazim is 0.3%, and the soaking depth is approximately 3 cm.

[0016] Beneficial effects of this invention:

[0017] 1. Under natural conditions, Clematis florida seeds cannot germinate normally, with an extremely low germination rate or no germination at all. Propagation by cuttings can effectively overcome this difficulty. This invention significantly improves the rooting rate of cuttings by selecting appropriate cutting and propagation steps, achieving a survival rate of over 70%. It can produce a large number of Clematis seedlings using limited parent plant resources in a short period, which is beneficial to the industrial development of this highly ornamental vine, Clematis florida, and has broad market prospects.

[0018] 2. This invention requires no machinery, is simple to operate, and does not use hormones, resulting in low cost and no pollution or phytotoxicity risks, making it suitable for practical production applications. It can reduce the harvesting of wild Clematis florida, provide high-quality materials for landscaping projects and home gardening, and offer a stable and abundant source of medicinal herbs and technical support for the traditional Chinese medicine industry. Specific implementation methods

[0019] Example 1

[0020] A method to improve the survival rate of Clematis grandiflora cuttings, the specific steps are as follows:

[0021] 1. Selection of cuttings: In May, cuttings were collected from Clematis florida cultivated in the nursery on the campus of Northeast Forestry University in Harbin, Heilongjiang Province.

[0022] 2. Cutting method: Select vigorous and healthy top cuttings with plump and healthy buds. Discard the top bud, leave 2 small leaves, and make small cuttings 6-8 cm long. Cut parallel about 1 cm above the first bud, and make a 45° angled cut at the bottom. Place the cut cuttings in a conical bottle filled with clean water to keep them moist for later use. Bundle 32 cuttings together and place them in the bottle.

[0023] 3. Sterilize the substrate and place it in a ventilated place to dry. One day before cutting, mix the substrate according to the ratio and add carbendazim powder. After mixing thoroughly, put it into the seedling pot and water it thoroughly.

[0024] 4. Cuttings: Make a guide hole about 3 cm deep in the substrate in advance. Take the cutting out of the water, place it at an angle with the bottom down, and soak it in carbendazim for 10 minutes. After taking it out and washing it, use the straight insertion method to put the cutting into the small hole in the substrate, press it gently to compact the substrate, and water it thoroughly.

[0025] 5. Post-propagation management: After cutting, place the seedling pots on the floor indoors where there is no direct sunlight. Cover each seedling pot with a shade net with 50% light transmittance. Spray water daily using a sprayer, adjusting the amount of water according to the degree of leaf wilting. Simultaneously, water the substrate to ensure the humidity of the Clematis florida cuttings is around 90%. Spray more water in the first few days, and reduce the amount of water appropriately after the cuttings have stabilized to prevent rotting of the cuttings. After 10 days, move the seedling pots to a greenhouse at 25-30°C and set the light duration to 10 hours to simulate the natural growth pattern of the plant for photosynthesis, but continue to cover with shade nets. Clean up dead branches and leaves promptly every day, and spray with a 0.3% carbendazim solution for disinfection every 10 days.

[0026] Furthermore, the matrix ratio is perlite:vermiculite:sand = 1:1:1.

[0027] After 40 days of cuttings, the average rooting rate was 73.93% and the average survival rate was 77.06%.

[0028] Example 2

[0029] 1. Selection of cuttings: In May, cuttings were collected from Clematis florida cultivated in the nursery on the campus of Northeast Forestry University in Harbin, Heilongjiang Province.

[0030] 2. Cutting method: Select vigorous and healthy top cuttings with plump and healthy buds. Discard the top bud, leave 2 small leaves, and make small cuttings 6-8 cm long. Cut parallel about 1 cm above the first bud, and make a 45° angled cut at the bottom. Place the cut cuttings in a conical bottle filled with clean water to keep them moist for later use. Bundle 32 cuttings together and place them in the bottle.

[0031] 3. Sterilize the substrate and place it in a ventilated place to dry. One day before cutting, mix the substrate according to the ratio and add carbendazim powder. After mixing thoroughly, put it into the seedling pot and water it thoroughly.

[0032] 4. Cuttings: Make a guide hole about 3 cm deep in the substrate in advance. Take the cutting out of the water, place it at an angle with the bottom down, and soak it in carbendazim for 10 minutes. After taking it out and washing it, use the straight insertion method to put the cutting into the small hole in the substrate, press it gently to compact the substrate, and water it thoroughly.

[0033] 5. Post-propagation management: After cutting, place the seedling pots on the floor indoors where there is no direct sunlight. Cover each seedling pot with a shade net with 50% light transmittance. Spray water daily using a sprayer, adjusting the amount of water according to the degree of leaf wilting. Simultaneously, water the substrate to ensure the humidity of the Clematis florida cuttings is around 90%. Spray more water in the first few days, and reduce the amount of water appropriately after the cuttings have stabilized to prevent rotting of the cuttings. After 10 days, move the seedling pots to a greenhouse at 25-30°C and set the light duration to 10 hours to simulate the natural growth pattern of the plant for photosynthesis, but continue to cover with shade nets. Clean up dead branches and leaves promptly every day, and spray with a 0.3% carbendazim solution for disinfection every 10 days.

[0034] Furthermore, the matrix ratio is perlite:vermiculite = 1:1.

[0035] After 40 days of cuttings, the average rooting rate was 67.71% and the average survival rate was 72.92%.

Claims

1. A method for improving the survival rate of Clematis grandiflora cuttings, characterized in that: The specific steps are as follows: (1) Selection of cuttings: Select healthy, disease-free, and well-developed branches with plump buds from the top of the mother plant as scions; (2) Timing of cutting and propagation: Cuttings should be taken in May in spring, and the cutting and propagation should be carried out simultaneously; (3) Cutting method: Discard the terminal bud, make small cuttings of 6-8 cm, cut parallel about 1 cm above the first bud, and make a 45° oblique cut at the bottom. Keep 2 small leaves on each cutting. After cutting, bundle 32 cuttings together and put them in a bottle with clean water for storage. (4) Preparation before cutting: Sterilize the substrate and then place it in a ventilated place to dry. One day before cutting, mix the substrate according to the ratio and add carbendazim powder. After mixing thoroughly, put it into the seedling pot and water it thoroughly. (5) Cuttings: Beforehand, insert a guide hole about 3 cm deep in the substrate, take the cutting out of the water, immerse it in carbendazim for 10 minutes with the oblique side facing down, take it out and use the straight insertion method to put the cutting into the small hole in the substrate, press it gently to compact the substrate, and water it thoroughly. (6) Post-propagation management: After cutting, place the seedling pots on the floor indoors where there is no direct sunlight. Cover each seedling pot with a shade net with a light transmittance of 50%. Spray water with a sprayer every day. The amount of water sprayed can be determined according to the degree of wilting of the leaves. At the same time, water the substrate to ensure that the humidity of the Clematis florida cuttings is about 90%. Spray more water in the first few days. After the cuttings have stabilized, reduce the amount of water appropriately to prevent the cutting branches and leaves from rotting. After 10 days, move the seedling pots to the light, but still cover them with shade nets. Clean up dead branches and leaves in time every day. Spray 0.3% carbendazim solution for disinfection every 10 days.

2. The method for improving the survival rate of Clematis grandiflora cuttings according to claim 1, characterized in that: The matrix ratio in step (4) is perlite: vermiculite: sand = 1:1:

1.

3. The method for improving the survival rate of Clematis grandiflora cuttings according to claim 1, characterized in that: In step (5), the concentration of carbendazim is 0.3% and the soaking depth is about 3 cm.