A miticidal composition and use thereof

Abstract

The present application belongs to a miticide composition and its application, the miticide composition comprises active ingredient A and active ingredient B, the active ingredient A is formula I compound: (formula I), the active ingredient B is any one of ethyl azamethylnitrile, azocyclotin, spirotetramat or cypofrifuram, the mass ratio of the active ingredient A and active ingredient B is 1:78-60:1.The miticide composition of the present application has excellent control effect on common crop mites, has significant control effect on common Tetranychidae mites and Eriophyidae mites, slows down the generation and development of mite resistance, effectively controls the damage of agricultural phytophagous mites, and prolongs the service life of existing miticides.

Description

[0001] This invention application is a divisional application of application number 202310612838.5, filed on May 29, 2023, entitled "An acaricide composition and its application". Technical Field

[0002] This invention belongs to the field of pesticide acaricide technology, and discloses an acaricide composition and its application. Background Technology

[0003] Compound I, chemically named (4′-chloro-6-fluoro-4-methyl-[1,1′-biphenyl]-3-yl)(2,2,2-trifluoroethyl) thioether, is a thioether acaricide effective against mites at all stages of their life cycle. Its structural formula is as follows: .

[0004] Ethoxam, CAS Registry Number: 1253429-01-4, is an acrylonitrile acaricide and belongs to the mitochondrial complex II electron transport inhibitors.

[0005] Azoxystrobin, CAS Registry Number: 134098-61-6, belongs to the mitochondrial complex I electron transport inhibitor class. It has knockdown and molting inhibition effects, but no systemic action. It has a strong contact killing effect on a variety of mites, with good rapid action and long residual effect. It has good control effect on all growth stages of mites and does not exhibit cross-resistance with other pesticides.

[0006] Spirotetramat, CAS Registry No. 203313-25-1, is a quaternary ketoacid acaricide developed by Bayer. Spirotetramat has unique bidirectional systemic properties, allowing it to move up and down throughout the plant, reaching new stems, leaves, and roots, effectively controlling various piercing-sucking insects and mites.

[0007] Acrylonitrile, CAS Registry Number: 560121-52-0, belongs to the mitochondrial succinate dehydrogenase inhibitor (SDHI). It is a novel acrylonitrile acaricide developed by Nissan Chemical Industries, Ltd. of Japan. This acaricide can be used to control various mites on fruit trees, citrus trees, tea trees, vegetables and other crops.

[0008] Agricultural plant pests are characterized by their small size, rapid reproduction, short development period, limited range of movement, strong adaptability, high mutation rate, and susceptibility to pesticide resistance. Prolonged application of insecticides and acaricides in agricultural production has led to a year-on-year increase in pesticide resistance among mites. These factors have made mite control increasingly difficult, significantly reducing the effectiveness of acaricides against mites. Meanwhile, the dosage used in the field continues to rise, posing a threat to environmental safety and further increasing pesticide residues in agricultural products. Summary of the Invention

[0009] In view of the shortcomings of the existing technology, the present invention provides an acaricidal composition that has excellent control effect on common crop pests such as spider mites and gall mites, and has a significant synergistic effect.

[0010] To achieve the above objectives, the present invention adopts the following technical solution: an acaricidal composition, wherein the acaricidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I: (Formula I), wherein the active ingredient B is any one of etoxazole, azoxystrobin, spirotetramat, or cyprodinil.

[0011] Furthermore, the mass ratio of active ingredient A to active ingredient B in the acaricidal composition is 1:78 to 60:1; Furthermore, the active ingredient B in the acaricide composition is etoxazole, and the mass ratio of active ingredient A to active ingredient B is 1:35~50:1; Furthermore, the active ingredient B in the acaricide composition is etoxazole, and the mass ratio of active ingredient A to active ingredient B is 1:15 to 40:1. Furthermore, the active ingredient B is azoxystrobin, and the mass ratio of active ingredient A to active ingredient B is 1:55~40:1; Furthermore, the active ingredient B is azoxystrobin, and the mass ratio of active ingredient A to active ingredient B is 1:28~34:1; Furthermore, the active ingredient B is spirotetramat, and the mass ratio of active ingredient A to active ingredient B is 1:78~45:1; Furthermore, the active ingredient B is spirotetramat, and the mass ratio of active ingredient A to active ingredient B is 1:52 to 10:1; Furthermore, the active ingredient B is cypermethrin, and the mass ratio of active ingredient A to active ingredient B is 1:55~40:1; Furthermore, the active ingredient B is cypermethrin, and the mass ratio of active ingredient A to active ingredient B is 1:33~20:1; Furthermore, in addition to the active ingredient, the acaricide composition also contains agriculturally acceptable auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers. Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergents, emulsifiers 2000 series, and wetting and penetrating agents F; and / or The dispersant is selected from one or more of the following: lignin sulfonates, alkyl naphthalene sulfonates formaldehyde condensates, naphthalene sulfonates, tristyrylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensates sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or The emulsifier is selected from one or more of the following: calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica; and / or The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or Antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or Defoamer selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of the fatty alcohols; and / or The solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil, vegetable oil derivatives, and water; and / or The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or The stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or Synergists are selected from synergistic phosphorus, synergistic ether; and / or The carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives. Furthermore, based on the total weight of the acaricide composition, the total weight of active ingredient A and active ingredient B accounts for 1% to 80% of the total weight of the acaricide composition.

[0012] Furthermore, the acaricidal composition can be prepared into a pesticide-permitted formulation, wherein the formulation is a solid formulation and / or a liquid formulation; Furthermore, the solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules; The liquid formulations include soluble agents, colloids, oils, spreading oils, emulsions, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspension agents, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions. Furthermore, the solid formulation is selected from water-dispersible granules or wettable powders; the liquid formulation is selected from suspension concentrates, emulsifiable concentrates, dispersible oil suspensions, or water emulsions.

[0013] The present invention also discloses the use of the acaricidal composition described above for the control of crop pests.

[0014] Furthermore, the crops mentioned are food crops and cash crops; Furthermore, the food crops are cereal crops, tuber crops, and edible legume crops, and the cash crops are fiber crops, oil crops, sugar crops, beverage crops, medicinal crops, dye crops, ornamental crops, fruits, and vegetables; Furthermore, the harmful mites are spider mites and / or malariae mites; Furthermore, the spider mite pests mentioned are *Tetranychus carmineus*, *Tetranychus two-spotted*, *Tetranychus citrus*, *Tetranychus truncatus*, and *Tetranychus kamizawa*; the gall mite pests mentioned are *Tetranychus citrus* and *Tetranychus lycopersicum*. Furthermore, the spider mite pests mentioned are *Tetranychus carmineus*, *Tetranychus two-spotted*, *Tetranychus citrus*, and *Tetranychus truncatus*; the gall mite pests mentioned are *Tetranychus citrus* rust mite.

[0015] Furthermore, the acaricidal composition is applied to the medium in which the mites occur.

[0016] The beneficial effects of this invention are as follows: 1) The acaricide composition of the present invention rationally combines compounds with different mechanisms of action, which further improves the control effect on crop mites, and its control effect is significantly higher than that of single agents; 2) The acaricide composition of the present invention can delay the emergence and development of drug resistance in harmful mites, and has good rapid effect and long-lasting effect; 3) The acaricide composition of the present invention does not cause phytotoxicity to crops and is safe for the natural enemies of mites, reducing the amount of pesticides used, is environmentally safe, and has agricultural promotion value. Detailed Implementation

[0017] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0018] Formulation preparation example: Preparation Example 1: 27% Formula I compound·etoxazole suspension (1:8) Formula: 3% Formula I compound, 24% etoxazole, 1% alkylphenol polyoxyethylene ether, 2% alkylaryl polyoxyethylene ether polyoxypropylene ether, 3% alkylphenol polyoxyethylene ether phosphate, 2% sodium lignosulfonate, 1.5% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium benzoate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance; Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0019] Preparation Example 2: 30% Formula I compound·etoxazole water-dispersible granules (1:5) Formula: 5% Compound I, 25% Ethoxadiazon, 8% Sodium polycarboxylate, 8% Naphthalenesulfonate formaldehyde condensate, 2% Sodium dodecyl sulfate, 5% precipitated silica, 25% starch, and kaolin as balance; Preparation method: According to the formulation ratio in the example, add the active ingredient to the carrier, and add surfactants and other functional additives therein, mix, and after air jet pulverization, add 10-25% water, and then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0020] Preparation Example 3: 24% Formula I compound·etoxazole wettable powder (3:1) Formula: 18% Compound I, 6% Ethoxadiazon, 3% Sodium lignosulfonate, 4% Sodium alkyl polyoxyethylene ether sulfonate, 1% BX (a type of bleaching powder), 5% fumed silica, and kaolin to make up the balance. Preparation method: The active ingredients, dispersant, wetting agent and filler are mixed according to the formula ratio, stirred evenly in a stirring tank, and then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0021] Preparation Example 4: 22% Formula I compound·azithromycin suspension (1:10) Formula: 2% Compound I, 20% Acaricide, 1% Isothiazine polyoxyethylene ether, 1% Sodium lignosulfonate, 3% Fatty alcohol polyoxyethylene ether sulfate, 2% Alkyl aryl polyoxyethylene ether polyoxypropylene ether, 0.5% Magnesium aluminum silicate, 0.2% Xanthan gum, 4% Propylene glycol, 1% Sodium sorbate, 0.5% Silicone oil, deionized water to make up the balance; Preparation method: Same as in preparation example 1.

[0022] Preparation Example 5: 18% Compound I·Azoxystrobin Emulsifiable Concentrate (2:1) Formula: 12% Compound I, 6% Azoxystrobin, 10% Dimethyl sulfoxide, 10% Tristyrene-phenol polyoxyethylene ether, 2% Calcium dodecylbenzenesulfonate, 15% Propylene carbonate, xylene balance; Preparation method: According to the formulation ratio of the preparation example, the measured active ingredients, solvents and co-solvents are added to the mixing tank and stirred to dissolve them. Then, the emulsifier is added, and the remaining solvent is used to make up the balance. The mixture is stirred evenly in the mixing tank and filtered to obtain the emulsifiable oil required by the present invention.

[0023] Preparation Example 6: 12% Compound I·Azoxystrobin aqueous emulsion (1:5) Formula: 2% Compound I, 10% Azoxystrobin, 8% Alkyl aryl polyoxyethylene ether polyoxypropylene ether, 1% Fatty alcohol polyoxyethylene ether sulfate, 20% Cyclohexanone, 0.2% Xanthan gum, 5% Glycerol, 0.1% Sodium benzoate, 0.5% Silicone oil, deionized water to make up the balance; Preparation method: According to the formulation ratio in the preparation example, the active ingredient is dissolved in the solvent and an emulsifier is added to form a homogeneous oil phase. Deionized water and antifreeze are mixed together to form a homogeneous aqueous phase. Under high-speed shearing, the oil phase is added to the aqueous phase. Finally, thickener, preservative and defoamer are added to form a well-dispersed water emulsion product.

[0024] Preparation Example 7: 19.5% Formula I compound·spirotetramat suspension (1:12) Formula: 1.5% Formula I compound, 18% spirotetramat, 2% succinate sulfonate, 1% fatty alcohol polyoxyethylene ether sulfate, 2% styrene-phenol polyoxyethylene ether phosphate, 1% lignin sulfonate, 1% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 0.2% xanthan gum, 0.2% potassium benzoate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance; Preparation method: Same as in preparation example 1.

[0025] Preparation Example 8: 20% Formula I compound·spirotetramat water-dispersible granules (3:1) Formula: 15% Compound I, 5% Spirotetramethrin, 8% Naphthalenesulfonate formaldehyde condensate, 6% Sodium lignosulfonate, 2% Sodium dodecyl sulfate, 8% Ammonium sulfate, 10% Starch, and kaolin to make up the balance; Preparation method: Same as in preparation example 2.

[0026] Preparation Example 9: 20% Formula I compound·spirotetramat dispersible oil suspension (1:1) Formula: 10% Formula I compound, 10% spirotetramat, 3% Gelbert alcohol polyoxyethylene ether, 15% castor oil polyoxyethylene ether, 1% calcium dodecylbenzene sulfonate, 1% sodium lignosulfonate, 0.2% bentonite, soybean oil to make up the balance; Preparation method: According to the formulation ratio in the preparation example, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, vegetable oil is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0027] Preparation Example 10: 26% Formula I compound·pyridaben suspension (1:12) Formula composition: 2% Formula I compound, 24% acrylonitrile pyridaben, 1% sodium dodecyl sulfate, 2% succinate sulfonate, 4% alkylphenol polyoxyethylene ether phosphate, 1% sodium polycarboxylate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.1% sodium benzoate, 0.5% silicone oil, deionized water to make up the balance.

[0028] Preparation method: Same as in preparation example 1.

[0029] Preparation Example 11: 27% Formula I compound pyridaben water-dispersible granules (1:8) Formula composition: 3% Formula I compound, 24% cypermethrin, 8% sodium polycarboxylate, 10% sodium lignosulfonate, 2% sodium dodecyl sulfate, 5% silica, 30% starch, and kaolin to make up the balance; Preparation method: Same as in preparation example 2.

[0030] Preparation Example 12: 30% Formula I compound·pyridaben wettable powder (5:1) Formula composition: 25% Formula I compound, 5% cypermethrin, 3% sodium lignosulfonate, 5% sodium methylene bisnaphthalenesulfonate, 2% BX powder, 5% silica, and kaolin to make up the balance; Preparation method: Same as in preparation example 3.

[0031] Indoor activity test.

[0032] Example 1: Indoor bioactivity test of compound I with etoxazole, tebufenozide, spirotetramat or cyprodinil against harmful mites.

[0033] Test basis: The test was conducted in accordance with NY / T 1154.12-2008 "Guidelines for the determination of biological activity of pesticides in the laboratory - Insecticides - Part 12: Tetranychus slide immersion method".

[0034] Test target: Tetranychus cinnabarinus ( Tetranychus cinnabarinus citrus pseudomitr Panonychus citri Female adult mites of the genus *Mcgregor*.

[0035] Test agents: Formula I compound technical grade, etoxazole technical grade, cyclopyridaben technical grade, spirotetramat technical grade, and cyprodinil technical grade.

[0036] Test method: Select female adult mites that are kept indoors and are in the same physiological state. Cut a 2cm piece of double-sided tape and stick it to one end of a glass slide. Select healthy female adult mites and stick them onto the double-sided tape, 30 mites per slide. After treatment, place the mites in a container lined with a damp sponge, cover it, and place it at (25±1)℃. After 2 hours, perform microscopic examination, remove dead, injured, and inactive individuals, and replenish to 30 mites per slide.

[0037] The technical grade compound of Formula I was dissolved in N,N-dimethylformamide. The technical grades of etoxazole, pyridaben, spirotetramat, and cyprodinil were dissolved in acetone. The dissolved technical grades were then prepared as a stock solution. Five series of mass concentration gradients were prepared using 0.1% Tween-80 aqueous solution in equal proportions. Glass slides were immersed in the above solutions, gently shaken for 5 seconds, and then removed. Excess solution was absorbed with absorbent paper, and the slides were placed in a white porcelain dish lined with a damp sponge. The slides were then covered with a translucent plastic film. Each treatment was repeated four times, and a blank control containing no solutions (containing all organic solvents and emulsifiers) was included.

[0038] Feeding and observation: The treated mites were fed and observed at (25±1)℃ with a photoperiod L:D=(16:8)h. After 48 hours of treatment, the mites were checked for mortality, and the total number of mites and the number of dead mites were recorded.

[0039] Data statistics and analysis: Based on the survey data, the corrected mortality rates for each treatment were calculated using the following formula, and the results were rounded to two decimal places.

[0040]

[0041] In the formula: P — Mortality rate, expressed as a percentage (%); K —This indicates the number of dead insects, expressed in heads; N — This indicates the total number of insects treated, in units of heads.

[0042]

[0043] In the formula: P 1 —Adjusted mortality rate, in percentages (%); P t —The mortality rate is expressed as a percentage (%). P 0 — Mortality rate in blank control group, expressed as a percentage (%).

[0044] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0045] The DPS statistical analysis system was used to analyze the data and obtain the virulence regression equation, correlation coefficient, and LC. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0046] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0047] In the formula: ATI —Measured toxicity index of the mixture; S LC of standard acaricides 50 The unit is milligrams per liter (mg / L); M LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0048]

[0049] In the formula: TTI —Theoretical toxicity index of the mixture; TI A —A. Toxicity index of drug A; P A —The percentage content of drug A in the mixture, expressed as a percentage (%). TI B —Toxicity index of drug B; P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0050]

[0051] In the formula: CTC —Cotoxicity coefficient; ATI —Measured toxicity index of the mixture; TTI —Theoretical toxicity index of mixed preparations.

[0052] Co-toxicity coefficient of compound CTC ≥120 exhibits a synergistic effect; CTC≤80 It exhibits antagonistic effects; 80 < CTC <120 exhibits an additive effect.

[0053] The relevant indoor activity tests are shown in the table below: Table 1. Indoor bioactivity test of compound I and etoxazole in combination with Tetranychus cinnabarinus. Test reagents virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Compound (A) of Formula I y = 5.7298 + 1.4245x 0.9943 0.3074 / Ethoxamyl (B) y = 4.4955 + 1.5831x 0.9944 2.0829 / A:B=1:35 y = 4.7828 + 1.4843x 0.9987 1.4007 128.145 A:B=1:28 y = 4.8168 + 1.4717x 0.9876 1.3320 130.402 A:B=1:15 y = 5.0004 + 1.4935x 0.9998 0.9993 153.150 A:B=1:8 y = 5.2101 + 1.4192x 0.9976 0.7111 178.413 A:B=1:5 y = 5.3731 + 1.3556x 0.9995 0.5306 200.014 A:B=5:1 y = 5.8753 + 1.1918x 0.9984 0.1843 194.414 A:B=10:1 y = 5.8711 + 1.2490x 0.9978 0.2007 166.030 A:B=20:1 y = 5.8339 + 1.2219x 0.9954 0.2077 154.264 A:B=30:1 y = 5.7586 + 1.1511x 0.9962 0.2193 144.137 A:B=40:1 y = 5.7641 + 1.1832x 0.9941 0.2260 138.906 A:B=50:1 y = 5.6433 + 1.0529x 0.9971 0.2449 127.654 The results of the indoor test (Table 1) show that the compound of Formula I and etoxazole have a good control effect on the carmine spider mite within a suitable mass ratio range. The mass ratio of the compound of Formula I to etoxazole is 1:35~50:1, and the co-toxicity coefficient is greater than 120, which shows a synergistic effect.

[0054] Table 2. Indoor bioactivity test of compound I with abamectin against Tetranychus cinnabarinus. Test reagents virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Compound (A) of Formula I y = 5.7298 + 1.4245x 0.9943 0.3074 / Azoxystrobin (B) y = 2.7166 + 1.4881x 0.9944 34.2331 / A:B=1:55 y = 3.6617 + 1.4039x 0.9991 8.9778 128.353 A:B=1:45 y = 3.8177 + 1.4211x 0.9994 6.7915 148.287 A:B=1:30 y = 4.0659 + 1.3424x 0.9980 4.9644 151.218 A:B=1:20 y = 4.2939 + 1.5171x 0.9968 2.9203 187.397 A:B=1:15 y = 4.5148 + 1.4938x 0.9937 2.1125 205.186 A:B=1:5 y = 5.0255 + 1.3735x 0.9991 0.9582 184.215 A:B=5:1 y = 5.7404 + 1.1189x 0.9945 0.2179 168.985 A:B=10:1 y = 5.7735 + 1.1510x 0.9886 0.2128 158.758 A:B=20:1 y = 5.7979 + 1.2912x 0.9957 0.2410 133.869 A:B=30:1 y = 5.6960 + 1.1805x 0.9975 0.2573 123.417 A:B=40:1 y = 5.7008 + 1.1936x 0.9921 0.2587 121.768 The results of the indoor test (Table 2) show that the compound of formula I and azoxystrobin have a good control effect on Tetranychus carmine when the mass ratio is within a suitable range. The mass ratio of compound I to azoxystrobin is 1:55~40:1, and the co-toxicity coefficient is greater than 120, which shows a synergistic effect.

[0055] Table 3. Indoor bioactivity test of compound I with spirotetramat against Tetranychus cinnabarin. Test reagents virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Compound (A) of Formula I y = 5.7298 + 1.4245x 0.9943 0.3074 / Spirotetramethrin (B) y = 1.7373 + 1.5211x 0.9865 139.5978 / A:B=1:78 y = 3.1930 + 1.5397x 0.9923 14.9134 138.968 A:B=1:64 y = 3.6260 + 1.2840x 0.9949 11.7532 149.005 A:B=1:52 y = 3.4941 + 1.5340x 0.9921 9.5869 152.482 A:B=1:33 y = 3.8565 + 1.5577x 0.9966 5.4217 179.714 A:B=1:21 y = 4.2416 + 1.4229x 0.9932 3.4118 189.457 A:B=1:8 y = 4.6742 + 1.5417x 0.9979 1.6268 167.120 A:B=5:1 y = 5.8876 + 1.4172x 0.9924 0.2364 155.972 A:B=12:1 y = 5.7666 + 1.1889x 0.9980 0.2266 146.935 A:B=25:1 y = 5.6907 + 1.1218x 0.9954 0.2423 131.931 A:B=45:1 y = 5.8760 + 1.4542x 0.9957 0.2498 125.787 A:B=60:1 y = 5.6787 + 1.2408x 0.9986 0.2838 110.117 The results of the indoor test (Table 3) show that the compound of Formula I and spirotetramat have a good control effect on Tetranychus carmine when the mass ratio is within a suitable range. The mass ratio of the compound of Formula I to spirotetramat is 1:78~45:1, and the co-toxicity coefficient is greater than 120, which shows a synergistic effect.

[0056] Table 4. Indoor bioactivity test of compound I with cypermethrin against Tetranychus cinnabarin. Test reagents virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Compound (A) of Formula I y = 5.7298 + 1.4245x 0.9943 0.3074 / Cyclopyralid (B) y = 3.8010 + 1.5174x 0.9982 6.1682 / A:B=1:55 y = 4.1883 + 1.4672x 0.9982 3.5745 128.733 A:B=1:43 y = 4.2598 + 1.5696x 0.9973 2.9619 145.294 A:B=1:32 y = 4.3982 + 1.4671x 0.9967 2.5718 152.014 A:B=1:24 y = 4.6003 + 1.3823x 0.9995 1.9462 179.808 A:B=1:12 y = 4.8471 + 1.3726x 0.9987 1.2924 193.492 A:B=1:6 y = 4.9828 + 1.3704x 0.9975 1.0294 160.917 A:B=3:1 y = 5.8047 + 1.4024x 0.9985 0.2668 151.113 A:B=10:1 y = 5.8192 + 1.3535x 0.9994 0.2482 135.561 A:B=20:1 y = 5.7651 + 1.3209x 0.9915 0.2635 122.189 A:B=30:1 y = 5.7781 + 1.4083x 0.9926 0.2802 113.176 A:B=40:1 y = 5.7080 + 1.3523x 0.9966 0.2995 105.073 The results of the indoor test (Table 4) show that the compound of Formula I and cypermethrin have good control effects on Tetranychus carmine when the mass ratio is within a suitable range. The mass ratio of the compound of Formula I to cypermethrin is 1:55~20:1, and the co-toxicity coefficient is greater than 120, which shows a synergistic effect.

[0057] Table 5. Indoor bioactivity tests of compound I and etoxazole in combination with *Paecilomyces citrus*. Test reagents virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Compound (A) of Formula I y = 4.2207 + 1.5613x 0.9990 3.1558 / Ethoxamyl (B) y = 4.8227 + 1.5606x 0.9975 1.2991 / A:B=1:33 y = 4.8291 + 1.4291x 0.9959 1.3170 100.378 A:B=1:25 y = 4.8981 + 1.3633x 0.9927 1.1879 111.893 A:B=1:15 y = 4.9969 + 1.5327x 0.9984 1.0047 134.238 A:B=1:8 y = 5.0483 + 1.4991x 0.9971 0.9285 149.700 A:B=1:2 y = 4.9979 + 1.4958x 0.9990 1.0033 161.071 A:B=2:1 y = 4.8898 + 1.3341x 0.9938 1.2095 176.725 A:B=5:1 y = 4.7026 + 1.6179x 0.9992 1.5270 166.908 A:B=10:1 y = 4.6351 + 1.4096x 0.9983 1.8150 153.880 A:B=20:1 y = 4.6091 + 1.2558x 0.9887 2.0477 144.294 A:B=30:1 y = 4.5298 + 1.3344x 0.9999 2.2511 134.011 A:B=40:1 y = 4.5057 + 1.2764x 0.9970 2.4394 125.010 The results of the indoor test (Table 5) show that the compound of Formula I and etoxazole have good control effects on citrus parvovirus within a suitable mass ratio range. The mass ratio of the compound of Formula I to etoxazole is 1:15 to 40:1, and the co-toxicity coefficient is greater than 120, which shows a synergistic effect.

[0058] Table 6. Indoor bioactivity test of compound I with abamectin against *Pseudomonas citrus*. Test reagents virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Compound (A) of Formula I y = 4.2207 + 1.5613x 0.9990 3.1558 / Azoxystrobin (B) y = 4.5681 + 1.6474x 0.9984 1.8290 / A:B=1:28 y = 4.7139 + 1.5726x 0.9950 1.5202 122.083 A:B=1:14 y = 4.7509 + 1.5520x 0.9972 1.4470 130.044 A:B=1:6 y = 4.8253 + 1.4068x 0.9967 1.3311 146.185 A:B=1:3 y = 4.8368 + 1.5425x 0.9981 1.2759 160.187 A:B=3:1 y = 4.7390 + 1.5170x 0.9978 1.4860 179.767 A:B=8:1 y = 4.6898 + 1.4139x 0.9947 1.6573 176.215 A:B=16:1 y = 4.6229 + 1.4496x 0.9989 1.8203 166.272 A:B=20:1 y = 4.4947 + 1.5598x 0.9999 2.1084 144.680 A:B=34:1 y = 4.3939 + 1.6990x 0.9972 2.2737 135.977 The results of the indoor test (Table 6) show that the compound of formula I and abamectin have good control effects on citrus parchoides within a suitable mass ratio range. The mass ratio of compound I to abamectin is 1:28~34:1, and the co-toxicity coefficient is greater than 120, which shows a synergistic effect.

[0059] Table 7. Indoor bioactivity test of compound I with spirotetramat against *Pachycarpus citrinum*. Test reagents virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Compound (A) of Formula I y = 4.2207 + 1.5613x 0.9990 3.1558 / Spirotetramethrin (B) y = 1.4406 + 1.4654x 0.9986 268.5327 / A:B=1:52 y = 2.3956 + 1.3781x 0.9958 77.5975 133.787 A:B=1:48 y = 2.4697 + 1.3980x 0.9956 64.5517 153.156 A:B=1:32 y = 2.4347 + 1.5617x 0.9930 43.9194 172.317 A:B=1:24 y = 2.8308 + 1.4012x 0.9976 35.3253 174.204 A:B=1:12 y = 2.8451 + 1.5980x 0.9909 22.3107 161.155 A:B=1:5 y = 3.3894 + 1.4892x 0.9959 12.0645 148.236 A:B=5:1 y = 4.4228 + 1.3270x 0.9964 2.7223 138.783 A:B=10:1 y = 4.3914 + 1.3836x 0.9947 2.7533 125.933 A:B=20:1 y = 4.4350 + 1.2637x 0.9884 2.7994 118.298 A:B=40:1 y = 4.3928 + 1.2486x 0.9936 3.0639 105.543 The results of the indoor test (Table 7) show that the compound of Formula I and spirotetramat have a good control effect on citrus psyllids when the mass ratio is within a suitable range. The mass ratio of the compound of Formula I to spirotetramat is 1:52~10:1, and the co-toxicity coefficient is greater than 120, which shows a synergistic effect.

[0060] Table 8 shows the indoor bioactivity test of compound I in combination with cyprodinil against *Pseudomonas citrus*. Test reagents virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Compound (A) of Formula I y = 4.2207 + 1.5613x 0.9990 3.1558 / Cyclopyralid (B) y = 4.5383 + 1.5050x 0.9975 2.0266 / A:B=1:32 y = 4.7011 + 1.4438x 0.9989 1.6108 127.192 A:B=1:28 y = 4.7899 + 1.3408x 0.9980 1.4334 143.150 A:B=1:16 y = 4.8379 + 1.3656x 0.9983 1.3144 157.499 A:B=1:8 y = 4.8809 + 1.4061x 0.9972 1.2153 173.662 A:B=2:5 y = 4.8715 + 1.4695x 0.9975 1.2231 184.562 A:B=3:4 y = 4.9415 + 1.2940x 0.9890 1.1097 215.704 A:B=5:1 y = 4.7224 + 1.5962x 0.9986 1.4925 193.477 A:B=10:1 y = 4.6818 + 1.3329x 0.9995 1.7326 173.361 A:B=20:1 y = 4.6256 + 1.3892x 0.9869 1.8601 165.272 A:B=30:1 y = 4.5507 + 1.3176x 0.9997 2.1927 141.382 A:B=40:1 y = 4.5710 + 1.2099x 0.9982 2.2623 137.625 The results of the indoor test (Table 8) show that the compound of Formula I and cypermethrin have good control effects on citrus parchoides within a suitable mass ratio range. The mass ratio of the compound of Formula I to cypermethrin is 1:32~40:1, and the co-toxicity coefficient is greater than 120, which shows a synergistic effect.

[0061] Field efficacy control trials.

[0062] Example 2: Field efficacy test of acaricide composition for controlling cotton red spider mites.

[0063] Experimental site: Walong Village, Shengli Town, Dongzhi County, Anhui Province. The experimental site is a plain with slight hills. The cotton is growing at a moderate rate, the soil fertility is moderate to high, and the cultivation and management conditions of each plot are basically the same.

[0064] Experimental target: cotton red spider mite.

[0065] Experimental crop: Cotton (Eza Cotton No. 10).

[0066] Test reagents: The test reagents are listed in the table below.

[0067] Plot arrangement: The experimental reagent, control reagent, and blank control were arranged in a randomized block design, with a plot area of ​​30m². 2 Each treatment was repeated 4 times.

[0068] Experimental method: On August 12, 2020, cotton was sprayed with pesticide using conventional manual spraying. Surveys were conducted once one day before application, and again on the 3rd, 7th and 14th days after application, for a total of 4 surveys.

[0069] Survey methods: A five-point sampling method and tagging method were adopted. Ten cotton plants were surveyed at each plot, and five leaves from different parts of each plant were tagged and marked. A total of 50 leaves from each plant in each plot were tagged and marked for the survey to investigate the initial insect population and the number of surviving insects.

[0070] Methods for calculating drug efficacy:

[0071]

[0072] The test results are shown in Table 9: Table 9. Results of field efficacy trials of the acaricide composition for controlling cotton red spider mites. deal with <![CDATA[Dosage of active ingredient g / hm 2 > Reduction rate 3 days after medication 3-day efficacy after medication (%) Reduction rate 7 days after medication 7 days after medication, the efficacy (%) Reduction rate 14 days after medication 14 days after medication, the efficacy (%) 27% Formula I compound • etoxazole suspension (1:8) 45 86.68 87.24 91.56 92.50 86.90 89.90 12% Formula I compound • azoxystrobin water emulsion (1:5) 50 87.69 88.21 90.00 91.11 94.18 95.51 20% Formula I compound • Spirotetramethrin water-dispersible granules (3:1) 60 85.99 86.58 87.61 88.99 89.10 91.60 27% Formula I compound • cypermethrin water-dispersible granules (1:8) 40 83.23 83.94 86.37 87.89 91.01 93.07 30% etoxazole suspension 40 77.90 78.84 81.34 83.42 69.51 76.51 20% azoxystrobin suspension 50 75.45 76.49 79.55 81.84 75.70 81.28 22.4% Spirotetramethrin Suspension 70 57.49 59.28 61.09 65.43 62.48 71.09 20% Formula I compound suspension 50 80.81 81.62 82.90 84.81 82.98 86.88 30% Cyclopyralid Suspension 90 78.64 79.54 79.92 82.16 81.15 85.47 Water comparison / -4.42 - -12.55 - -29.78 - As shown in Table 9, the combination of compound I with etoxazole, acetamiprid, spirotetramat, or cyprodinil has a good control effect on cotton red spider mites, and compared with the control single agent, it has better rapid effect and longer duration of action.

[0073] Example 3: Field efficacy test of acaricide composition for controlling citrus paronychia.

[0074] Experimental basis: The experiment was conducted in accordance with GB / T 17980.11-2000 "Guidelines for Field Efficacy Tests of Pesticides (I) Control of Citrus Paronychia with Acaricides".

[0075] Experimental target: Citrus psyllid ( Panonychus citri Mcgregov).

[0076] Experimental crop: Citrus (Mandarin orange), 5 years old.

[0077] Experimental site: Citrus orchard in Gancao Village, Lingchuan County, Guilin City, Guangxi Zhuang Autonomous Region. The experimental site has moderate fertility, no weeds or other coverings on the ground, and the cultivation conditions of all experimental plots are uniform and consistent with local scientific agricultural practices.

[0078] Experimental plot arrangement: The experimental agent, control agent and blank control were arranged in a randomized block design, with each plot containing 2 fruit trees and each treatment replicated 4 times.

[0079] Experimental method: A 3WBD-16 backpack sprayer was used to evenly spray the stems and leaves of citrus trees, wetting the leaves until the solution dripped. The application was performed once during the initial peak period of the citrus paronychia mite. The number of live mites was recorded at 3 days and 15 days after application.

[0080] Survey method: For each plot, tender shoots were marked in five directions (east, west, south, north, and center) on the trees, and the number of live mites on a total of 50 leaves was investigated. The leaf surface was directly observed using a handheld magnifying glass, and the number of mites was counted.

[0081] Methods for calculating drug efficacy:

[0082]

[0083] The test results are shown in the table below: Table 10 Results of field efficacy trials of acaricide compositions against *Pseudomonas citrus*.

[0084] The results of the above field efficacy tests show that the acaricidal composition of the present invention has a good control effect on citrus psyllids, and its control efficacy is significantly higher than that of the control single agent.

[0085] Example 4: Field efficacy test of acaricide composition for controlling citrus rust mites.

[0086] Environmental conditions: Field trials were conducted in a citrus orchard in Beishan Village, Zhongguan Town, Yuexi County, Anqing City, Anhui Province. Field management was carried out according to conventional methods. The soil fertility of the experimental site was moderate to high. The cultivation conditions of all experimental plots were uniform and consistent with local scientific agricultural practices.

[0087] Test subjects and crops / variety: The test subject was the citrus rust mite, and the test crop was citrus (Red Beauty).

[0088] Plot arrangement: The experimental agent, control agent and blank control were arranged in a randomized block design. The plot area was 2 fruit trees. A protection row was set up around each plot. Each treatment was repeated 4 times.

[0089] Experimental Methods: A Gongnong-16 backpack sprayer was used for application. The amount of pesticide applied was such that the leaves and fruits were moistened, with a slight dripping of pesticide from the leaves. Leaves were randomly sampled using a 10x handheld magnifying glass. Once an average of 3-5 live mites were observed per field of view, one application was made. No other insecticides or acaricides were used during the experiment, and other agricultural operations were carried out normally.

[0090] Investigation methods: A baseline population was surveyed before pesticide application, and the number of live mites was recorded at 3, 7, 14, and 21 days after application. Two fruit trees were surveyed in each plot, and two affected leaves from the same shoot stage were marked on the east, west, south, north, and center of each tree. A total of 20 leaves were surveyed in each plot, covering two fields of view, and the number of live mites was calculated.

[0091] Methods for calculating drug efficacy:

[0092]

[0093] The test results are shown in the table below: Table 11 Results of field efficacy trials of acaricide compositions for controlling citrus rust mites deal with Dilution factor Reduction rate 3 days after medication 3-day efficacy after medication (%) Reduction rate 7 days after medication 7 days after medication, the efficacy (%) Reduction rate 14 days after medication 14 days after medication, the efficacy (%) Reduction rate 21 days after medication 21 days after administration, efficacy (%) 24% Formula I compound • etoxazole wettable powder (3:1) 4500 81.57 82.47 84.17 86.47 93.57 95.08 89.74 92.78 22% Formula I compound • azoxystrobin suspension (1:10) 4500 82.14 83.02 84.71 86.93 89.69 92.12 87.66 91.32 20% Formula I compound • Spirotetramethrin water-dispersible granules (3:1) 4500 85.42 86.14 89.86 91.33 89.06 91.64 86.85 90.75 27% Formula I compound • cypermethrin water-dispersible granules (1:8) 4000 83.75 84.55 85.97 88.00 90.40 92.66 93.06 95.12 30% etoxazole suspension 3000 74.80 76.04 77.01 80.34 81.73 86.04 76.85 83.72 20% azoxystrobin suspension 4000 71.13 72.55 76.14 79.60 79.82 84.58 73.34 81.25 22.4% Spirotetramethrin Suspension 4000 65.84 67.52 65.02 70.09 62.96 71.70 56.52 69.41 20% Formula I compound suspension 3000 80.49 81.45 81.07 83.81 82.66 86.75 78.03 84.55 30% Cyclopyralid Suspension 3000 69.62 71.11 71.45 75.58 71.45 78.18 71.45 79.92 Water comparison - -5.17 - -16.93 - -30.85 - -42.16 - As shown in Table 11, the experimental results indicate that the rational combination of compound I with etoxazole, fenpyroximate, spirotetramat, and cypermethrin exhibits good control effects against citrus rust mites, effectively controlling the damage caused by the mites. Three days after application, the control efficacy was significantly higher than the single-agent control, demonstrating good rapid-acting properties; 21 days after application, the control efficacy of each compound formulation was greater than 90%, with a relatively long residual effect.

[0094] During the above field efficacy trials, irregular observations were conducted, and no visible phytotoxicity symptoms were found in the crops tested by any of the formulations in the examples. The crops grew well after application.

[0095] The results of the above indoor and field efficacy tests clearly show that the compound of Formula I in this invention, when combined with etoxazole, fenpyroximate, spirotetramat, or cyprodinil, exhibits excellent control effects against common crop mites at appropriate mass ratios. The synergistic effect is significant, and each acaricide composition has good rapid action, long-lasting effect, reduces the amount of pesticide used, does not cause phytotoxicity to crops, and is safe for the environment, making it suitable for widespread use.

[0096] It should be understood that the above embodiments are merely some embodiments of the present invention, provided only to better understand the embodiments of the present invention, and are not all embodiments of the present invention. In practical applications, by adjusting the content of each component and the composition of the components in the present invention, different and numerous embodiments can be obtained, all of which are within the scope of the present invention.

Claims

1. A mite-killing composition, characterized in that, The acaricide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I: (Formula I), wherein the active ingredient B is either azoxystrobin or spirotetramat, and the mass ratio of active ingredient A to active ingredient B in the acaricide composition is 1:78~60:

1.

2. The acaricide composition according to claim 1, characterized in that, The active ingredient B in the acaricide composition is azoxystrobin, and the mass ratio of active ingredient A to active ingredient B is 1:55~40:

1. The active ingredient B is spirotetramat, and the mass ratio of active ingredient A to active ingredient B is 1:78~45:

1.

3. The acaricide composition according to claim 1, characterized in that, The active ingredient B in the acaricide composition is azoxystrobin, and the mass ratio of active ingredient A to active ingredient B is 1:28~34:

1. The active ingredient B is spirotetramat, and the mass ratio of active ingredient A to active ingredient B is 1:52 to 10:

1.

4. The acaricide composition according to claim 1, characterized in that, In addition to the active ingredient, the acaricide composition contains agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

5. The acaricide composition according to claim 1, characterized in that, The total weight of the acaricide composition is 100%, and the total weight of active ingredient A and active ingredient B accounts for 1% to 80% of the total weight of the acaricide composition.

6. The acaricide composition according to claim 1, characterized in that, The acaricidal composition can be prepared into pesticide-permitted formulations, wherein the formulation is a solid formulation and / or a liquid formulation.

7. The acaricide composition according to claim 6, characterized in that, The solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules. The liquid formulations include soluble agents, colloids, oils, spreading oils, emulsions, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspension agents, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions. Preferably, the solid formulation is selected from water-dispersible granules or wettable powders; the liquid formulation is selected from suspension concentrates, emulsifiable concentrates, dispersible oil suspensions, or water emulsions.

8. Use of the acaricidal composition according to any one of claims 1-7 for the control of crop pests.

9. The use according to claim 8, characterized in that, The pests mentioned are spider mites and / or gall mites.

10. The use according to claim 8, characterized in that, The acaricide composition is applied to the medium in which the mites occur.

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