A soothing repair composition containing Saint John's wort glycosides and its use in sensitive skin care

Abstract

The present application relates to a soothing repair composition containing Saintgrass nuclease and its application in sensitive skin care, belonging to the technical field of cosmetics. The soothing repair composition containing Saintgrass nuclease includes Saintgrass nuclease, chamomile extract, Sophora flavescens root extract and Magnolia kobus extract, which synergistically act on multiple links such as nerves, immunity and inflammation to comprehensively soothe sensitive symptoms, repair skin barrier, and the effect is significantly better than that of single component, is safe and non-irritating, and has a wide application prospect in the field of skin care.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to a soothing and repairing composition containing senna glycoside and its application in sensitive skin care. Background Technology

[0002] With environmental changes, increased life stress, and the effects of improper skincare practices, the incidence of sensitive skin is rising year by year. Sensitive skin typically manifests as an excessive sensory response to external stimuli (such as cosmetics, climate change, pollutants, etc.), such as stinging, burning, itching, and tightness, often accompanied by visible erythema and desquamation. From a physiological perspective, its core mechanisms involve impaired skin barrier function, hyperresponsiveness of nerve receptors (such as TRPV1), and a cascade amplification of immune inflammatory responses (such as NF-κB pathway activation and cytokine release).

[0003] Most soothing and repairing products on the market currently employ single or simple compound systems, primarily targeting hydration and moisturizing or specific inflammatory pathways. However, the causes of sensitive skin are complex, involving multiple interactions among the nervous, immune, vascular, and barrier functions. Therefore, developing a soothing and repairing composition that can simultaneously act on neurogenic inflammation, immune inflammation, and the amplification of inflammatory signals, providing comprehensive intervention through multiple pathways and targets, has significant practical implications and market value. Summary of the Invention

[0004] The purpose of this invention is to provide a soothing and repairing composition containing senna-based glycosides and its application in sensitive skin care. This invention combines senna-based glycosides, chamomile extract, sophora flavescens root extract, and magnolia buds extract. The four ingredients work synergistically to comprehensively soothe sensitive symptoms from multiple aspects such as the nervous, immune, and inflammatory levels, and repair the skin barrier. The effect is significantly better than that of a single ingredient, and it is safe and non-irritating, with broad application prospects in the skin care field.

[0005] The objective of this invention can be achieved through the following technical solutions: In a first aspect, the present invention provides a soothing and repairing composition containing senna-based glycosides, the soothing and repairing composition comprising the following components: senna-based glycosides, chamomile extract, sophora flavescens root extract, and magnolia buds extract, wherein the weight ratio of senna-based glycosides, chamomile extract, sophora flavescens root extract, and magnolia buds extract is (0.1-1):(0.5-5):(0.1-1):(0.5-5); Among them, senna-based glycosides significantly downregulate the release of downstream inflammatory factors TNF-α, IL-1β, and IL-6 by inhibiting the activation of the NF-κB signaling pathway; at the same time, by inhibiting the activity of cyclooxygenase-2 (COX-2) and lipoxygenase (LOX), it reduces the production of inflammatory mediators such as prostaglandins and leukotrienes, thus blocking the immune inflammation response from the source. Components such as tetrastigmoyl spermidine in chamomile extract can competitively bind to NK-1 receptors, blocking the binding of substance P to NK-1 receptors, thereby effectively inhibiting neurogenic inflammatory responses mediated by substance P. This blocking effect not only reduces the release of vasoactive peptides and decreases capillary permeability, relieving redness and burning sensation, but also downregulates the expression of downstream pro-inflammatory cytokines (such as TNF-α and IL-6), thereby inhibiting the inflammatory cascade response from the source. Oxymatrine in Sophora flavescens root extract reduces the production of heat shock protein 60 (HSP60) at the transcriptional level by inhibiting the activity of heat shock factor 1 (HSF1). HSP60 is an endogenous danger signal that can activate immune cells to amplify inflammation. By reducing HSP60, the vicious cycle of inflammation self-amplification is broken. Magnolia flowers belong to the Magnolia genus. Components such as magnoflorine and magnolol can inhibit the excessive opening of TRPV1, reduce calcium ion influx, and thus directly reduce the burning and stinging sensations caused by external stimuli (such as heat, acid, and chemicals), reduce the release of inflammatory factors triggered by these stimuli, and increase the skin's sensory threshold.

[0006] Preferably, the weight ratio of the senna-based glycoside, chamomile extract, sophora root extract, and magnolia extract is (0.2-0.5):(1-3):(0.2-0.8):(1-3).

[0007] Secondly, the present invention provides the application of the soothing and repairing composition containing senna glycoside described in the first aspect in the preparation of a sensitive skin care product, wherein the sensitive skin care product includes at least one of toner, lotion, cream, mask, and spray, and the amount of the soothing and repairing composition containing senna glycoside added to the sensitive skin care product is 3%-8%.

[0008] Thirdly, the present invention provides an emulsion comprising: a soothing and repairing composition containing senna-containing senna as described in the first aspect, a moisturizer, a thickener, a chelating agent, an emulsifier, an oil, a preservative, a pH adjuster, and a solvent.

[0009] Fourthly, the present invention provides a method for preparing the emulsion described in the third aspect, comprising the following steps: S1. Mix and stir the humectant, thickener, chelating agent and solvent, heat to 75-85℃, homogenize at 6000-7000 rpm for 4-6 minutes, keep warm after homogenization, and obtain the first mixture; S2. Mix the emulsifier and oil, heat to 75-85℃, and homogenize at 6000-7000 rpm for 4-6 minutes. After homogenization, keep warm for later use to obtain the second mixture. S3. Mix and stir different preservatives, heat to 55-65℃ to melt, and obtain the third mixture; S4. Heat the first mixture to 75-85℃, add the second mixture, homogenize at 7500-8500 rpm for 2-4 minutes, then cool to 45-55℃, add the third mixture at 200-400 rpm and mix. Then cool to below 45℃, add the soothing and repairing composition containing senna-based glycosides as described in the first aspect, continue stirring for 4-6 minutes, finally add a pH adjuster to adjust the pH to 5.0-6.5, stop stirring, discharge the material, and obtain the emulsion.

[0010] The beneficial effects of this invention are: This invention provides a soothing and repairing composition containing senna-based glycosides, which is composed of senna-based glycosides, chamomile extract, sophora flavescens root extract, and magnolia buds extract.

[0011] This invention, based on the complex mechanisms of sensitive skin, constructs a multi-target synergistic network through the scientific formulation of four active ingredients. Specifically, senna-based glycosides inhibit the NF-κB pathway and key inflammatory enzymes; chamomile extract blocks substance P-mediated neurogenic inflammation; sophora root extract interrupts inflammation amplification by inhibiting HSP60; and magnolia extract inhibits TRPV1 overactivation, reducing nerve sensitivity. The synergistic effect of these four ingredients comprehensively soothes sensitivity symptoms from multiple aspects, including the nervous, immune, and inflammatory levels, repairing the skin barrier. The effects are significantly superior to those of single ingredients, and the product is safe and non-irritating, showing broad application prospects in the skincare field. Detailed Implementation

[0012] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0013] The herbicide was purchased from Xi'an Shilin Biotechnology Co., Ltd., and its product name is herbicide. Chamomile extract was purchased from Guangzhou Meiyi Biotechnology Co., Ltd., and its product name is chamomile extract. The Sophora flavescens root extract was purchased from Shaanxi Dongjiang Kangtai Health Industry Co., Ltd., and its product name is Sophora flavescens root extract. Magnolia sieboldii extract was purchased from Guangzhou Jinbaolai Biotechnology Co., Ltd., and its product name is Magnolia Extract.

[0014] Unless otherwise specified, all other materials, reagents, etc. used in all embodiments and comparative examples of this invention are commercially available.

[0015] The components and their weights of the soothing and repairing compositions containing senna-containing glucosinolates in Examples 1-5 and Comparative Examples 1-6 are shown in Table 1, and the total weight of the soothing and repairing compositions in each group is equal. The preparation methods of the soothing and repairing compositions containing senna-containing glucosinolates in Examples 1-5 and Comparative Examples 1-6 include the following steps: The ingredients, including senna extract, chamomile extract, sophora root extract, and magnolia extract, were mixed in the specified weight ratios and stirred until homogeneous to obtain a soothing and repairing composition containing senna extract.

[0016] Table 1. Components and weight ratios of the composition Group Sacred herbaceous glycosides Chamomile extract Sophora flavescens root extract Magnolia officinalis extract Example 1 0.3 2 0.5 2 Example 2 0.2 1 0.2 1 Example 3 0.5 3 0.8 3 Example 4 0.1 0.5 0.1 0.5 Example 5 1 5 1 5 Comparative Example 1 / 2 0.5 2 Comparative Example 2 0.3 / 0.5 2 Comparative Example 3 0.3 2 / 2 Comparative Example 4 0.3 2 0.5 / Comparative Example 5 2 0.3 2 0.5 Comparative Example 6 0.01 1 0.01 1 The " / " indicates that the ingredient is not added.

[0017] Comparative Example 7 Compared with Example 1, the difference is that Comparative Example 7 uses an equal amount of hesperidin (purchased from Fufeng Sinote Biotechnology Co., Ltd.) instead of sennaoside, while the other components and their weight ratios are the same as in Example 1.

[0018] Comparative Example 8 Compared with Example 1, the difference is that Comparative Example 8 uses an equal amount of bisabolol (purchased from Yili Jiangning Biotechnology Co., Ltd.) to replace chamomile extract, while the other components and their weight ratios remain the same as in Example 1.

[0019] Comparative Example 9 Compared with Example 1, the difference is that Comparative Example 9 uses an equal amount of licorice root extract (purchased from Fufeng Sinote Biotechnology Co., Ltd.) instead of sophora flavescens root extract, while the other components and their weight ratios remain the same as in Example 1.

[0020] Comparative Example 10 Compared with Example 1, the difference is that Comparative Example 10 uses an equal amount of Magnolia officinalis extract (purchased from Fufeng Sinote Biotechnology Co., Ltd.) instead of Magnolia sieboldii extract, while the other components and their weight ratios are the same as in Example 1.

[0021] Test Case: Anti-inflammatory Effect Test S1. Plating: Logarithmically growing RAW264.7 cells were seeded into 24-well plates at a cell density of 2 × 10⁻⁶ cells / well. 5 Cells / mL, 0.5mL per well. After the plate is prepared, mark the cell plate cover (divided into blank control group, model group, positive control group, and experimental group). Incubate in a carbon dioxide incubator for 24 h. Each group has three replicates. S2. Sample processing: After cell incubation for 24 hours, aspirate the supernatant and add 0.5 mL of the following samples respectively: ① Blank control group: DMEM culture medium; ② Model group: DMEM medium containing 1 μg / mL LPS; ③ Positive control group: DMEM medium containing 0.001 wt% dexamethasone and 1 μg / mL LPS; ④ Experimental groups 1-15: containing 0.5 wt% of the soothing and repairing composition of Examples 1-5 or Comparative Examples 1-10 and 1 μg / mL LPS in DMEM medium; S3. Detection of inflammatory factors in cell supernatants of each group: After 24 hours of cell treatment, the supernatant was pipetted into centrifuge tubes, centrifuged at 1000 rpm for 5 minutes, the precipitate was discarded, and the supernatant was collected. The changes in inflammatory factors in the supernatant were detected using the Xinbosheng TNF-α inflammatory factor detection kit. The results were expressed as TNF-α inhibition rate. The formula for calculating the TNF-α inhibition rate is as follows: TNF-α inhibition rate (%) = (TNF-α level in model group - TNF-α level in experimental group) / TNF-α level in model group × 100%; The results, after being averaged, are recorded in Table 2 below.

[0022] Table 2 TNF-α inhibition rate / % Example 1 45.1 Example 2 41.9 Example 3 43.7 Example 4 36.5 Example 5 39.6 Comparative Example 1 13.8 Comparative Example 2 16.9 Comparative Example 3 17.6 Comparative Example 4 15.5 Comparative Example 5 25.4 Comparative Example 6 27.3 Comparative Example 7 20.0 Comparative Example 8 22.1 Comparative Example 9 22.8 Comparative Example 10 21.2 As shown in Table 2, compared with Comparative Examples 1-10, the soothing and repairing compositions containing senna-containing glucosinolates provided in Examples 1-5 can significantly inhibit the release of TNF-α induced by LPS in RAW264.7 cells, and have good anti-inflammatory effects.

[0023] The experimental results of comparative examples 1-5 and comparative examples 1-6 show that the four components of the composition of the present invention, namely, senna glycoside, chamomile extract, sophora flavescens root extract and magnolia buds extract, can exert a synergistic effect. When the weight ratio of senna glycoside, chamomile extract, sophora flavescens root extract and magnolia buds extract is (0.1-1):(0.5-5):(0.1-1):(0.5-5), the inhibitory effect of the composition on TNF-α reaches the optimal level. The absence of any one component or exceeding the ratio range limited in this application will lead to a significant decrease in the TNF-α inhibition rate.

[0024] Comparing the experimental results of Example 1 and Comparative Examples 7-10, it is evident that replacing any component in the composition of this invention with other known ingredients of similar efficacy significantly reduced the TNF-α inhibition rate. This demonstrates that the composition formed by the four active ingredients selected in this invention possesses unique and irreplaceable properties, and other single ingredients with similar efficacy cannot replicate the synergistic anti-inflammatory effect of the composition of this invention. This further verifies the uniqueness of the raw material selection in this invention, namely, that the four components form a complementary and synergistic mechanism of action, and any substitution or adjustment may result in the anti-inflammatory effect failing to achieve the expected results of this invention.

[0025] Application Examples 1-5 and Comparative Application Examples 1-10 The soothing and repairing compositions containing senna-containing glucosinolates from Examples 1-5 and Comparative Examples 1-10 were combined with other excipients to prepare emulsions for Application Examples 1-5 and Comparative Application Examples 1-10, respectively; in addition, an emulsion without the soothing and repairing composition was prepared as a blank application example; the formulations of the above emulsions are shown in Table 3.

[0026] Table 3 Emulsion components and dosage

[0027] The emulsion preparation methods of Application Examples 1-5 and Comparative Application Examples 1-10 include the following steps: S1. Mix each component of phase A with deionized water and stir. Heat to 80°C and homogenize at 6500 rpm for 5 minutes. After homogenization, keep warm for later use to obtain the first mixture. S2. Mix and stir the components of phase B, heat to 80°C, homogenize at 6500 rpm for 5 minutes, keep warm after homogenization, and obtain the second mixture. S3. Mix and stir the components of phase C, heat to 60°C until completely dissolved, to obtain the third mixture; S4. Heat the first mixture to 80°C, add the second mixture, homogenize at 8000 rpm for 3 minutes, then cool to 50°C, add the third mixture at 300 rpm and mix. Then cool to below 45°C, add the soothing and repairing composition containing senna glycosides, continue stirring for 5 minutes, finally add a pH adjuster to adjust the pH to 5.7, stop stirring, discharge the material, and obtain the emulsion.

[0028] The method for preparing the emulsion in the blank application example includes the following steps: S1. Mix each component of phase A with deionized water and stir. Heat to 80°C and homogenize at 6500 rpm for 5 minutes. After homogenization, keep warm for later use to obtain the first mixture. S2. Mix and stir the components of phase B, heat to 80°C, homogenize at 6500 rpm for 5 minutes, keep warm after homogenization, and obtain the second mixture. S3. Mix and stir the components of phase C, heat to 60°C to melt, and obtain the third mixture; S4. Heat the first mixture to 80°C, add the second mixture, homogenize at 8000 rpm for 3 minutes, then cool to 50°C, add the third mixture at 300 rpm and mix, then cool to below 45°C, add pH adjuster to adjust pH to 5.7, stop stirring, discharge the material, and obtain the emulsion.

[0029] Effect test 1. Skin soothing and repair effect test The emulsions prepared according to Application Examples 1-5, Comparative Application Examples 1-10, and the blank application example were used as test samples to test their skin soothing and repair effects. The method is as follows: Forty-eight volunteers aged 18-45 were selected and randomly divided into 16 groups of three. During the test, each group randomly used the lotion from Application Examples 1-5, Control Application Examples 1-10, and the blank application example of this invention. The lotion was used twice daily, morning and evening. The method of use was to apply 1 g of the sample evenly to the entire face after cleansing and moisturizing, and gently massage until fully absorbed. Follow-up visits were conducted on days 0 and 14 to test various indicators.

[0030] On days 0 and 14, subjects did not apply any skincare products. After cleansing, they sat quietly for 30 minutes in a constant temperature and humidity room with a temperature of 21±1℃ and a humidity of 50±10%. The initial TEWL value and moisture content of the subjects were measured using the Tewameter®™ Hex (Courage + Khazaka) transdermal moisture loss probe and the Corneometer® CM 825 (Courage + Khazaka) moisture probe. Facial images were taken using a Visia-7, and the exported red area images were analyzed to obtain the a* value. Analyze the improvement of each indicator, and take the average value of the data. The improvement is calculated as follows: Improvement rate (%) = | (X) 使用后 -X 使用前 ) / X 使用前 ×100%| In the formula: X 使用前 Data on various indicators tested before subjects used the product; X 使用后 Data on various indicators for subjects before they use the product.

[0031] The average test results are recorded in Table 4 below.

[0032] Table 4 TEWL value improvement rate / % Moisture content improvement rate / % a* value improvement rate / % Blank application example 2.2 3.3 2.5 Application Example 1 18.1 30.1 14.9 Application Example 2 16.7 27.8 13.6 Application Example 3 17.5 29.2 14.4 Application Example 4 15.4 23.5 11.5 Application Example 5 16.0 25.9 12.3 Comparative Application Example 1 6.7 7.4 4.6 Comparative Application Example 2 7.8 10.0 5.1 Comparative Application Example 3 8.1 10.8 5.4 Comparative Application Example 4 7.3 8.6 5.2 Comparative Application Example 5 10.9 13.7 7.3 Comparative Application Example 6 11.6 14.9 7.8 Comparative Application Example 7 9.4 11.4 5.7 Comparative Application Example 8 10.2 12.6 6.3 Comparative Application Example 9 10.7 13.2 6.7 Comparative Application Example 10 9.8 12.1 6.0 As shown in Table 4, compared with Comparative Application Examples 1-10, the soothing and repairing compositions containing senna glycosides provided in Application Examples 1-5 can significantly improve the skin barrier, enhance skin hydration, and reduce facial redness, demonstrating good soothing and repairing effects.

[0033] By comparing the improvement rates of Application Example 1 with those of Comparative Examples 1-10, it can be seen that the four components of the composition of the present invention—herbaceous glycoside, chamomile extract, sophora flavescens root extract, and magnolia dahurica extract—achieve a synergistic effect in soothing and repairing functions. The absence or replacement of any of these components, or the adjustment of the dosage ratio of each component, will result in a significant decrease in the improvement rate data.

[0034] 2. Sensitive skin suitability test Whether a product is suitable for people with sensitive skin can be determined by assessing the improvement rate of lactic acid stinging before and after using the product.

[0035] Volunteer selection: Ten people with sensitive skin were selected as volunteers (five volunteers used the same test sample) and divided into two groups; Test procedure: The test was conducted on the nasolabial folds of volunteers. 50 μL of 10% lactic acid solution was dropped onto a single layer of filter paper with a diameter of 0.8 cm. The subjects were asked about their stinging sensation at 0.5 min and 2.5 min. The subjects were scored using a 4-point scale (0 points for no stinging, 1 point for mild stinging, 2 points for moderate stinging, and 3 points for severe stinging). Sample usage: Volunteers in the experimental group applied sample 1 to the entire face, while volunteers in the blank control group applied sample 1 to the entire face. Volunteers applied the product twice daily, morning and evening, approximately 1 mL each time, for two consecutive weeks. After two weeks of use, the above test steps were repeated.

[0036] Record the lactic acid stinging scores of volunteers in different groups before and after 2 weeks of use, and calculate the improvement rate of lactic acid stinging in volunteers. The specific formula is as follows: Improvement rate of lactic acid stinging (%) = (sum of lactic acid stinging scores before use - sum of lactic acid stinging scores after 2 weeks of use) / sum of lactic acid stinging scores before use × 100%; The sum of lactic acid stinging scores = the average lactic acid stinging score over 0.5 minutes + the average lactic acid stinging score over 2.5 minutes; The higher the improvement rate of lactic acid stinging, the better the repair effect of the sample, and the more suitable the product is for people with sensitive skin. The test results are recorded in Table 5 below.

[0037] Table 5 Improvement rate of lactic acid stinging / % Blank application example 13.3 Application Example 1 70.59 As shown in Table 5, the soothing and repairing composition containing senna glycoside prepared in this invention can effectively improve lactic acid stinging after use. It is gentle and non-irritating, and suitable for people with sensitive skin.

[0038] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any indirect modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A soothing and repairing composition containing senna-based glycosides, characterized in that, The soothing and repairing composition containing senna glycosides comprises the following components: senna glycosides, chamomile extract, sophora flavescens root extract, and magnolia buds extract, wherein the weight ratio of senna glycosides, chamomile extract, sophora flavescens root extract, and magnolia buds extract is (0.1-1):(0.5-5):(0.1-1):(0.5-5).

2. The soothing and repairing composition containing senna-based glycosides according to claim 1, characterized in that, The weight ratio of the herbaceous glycoside, chamomile extract, sophora flavescens root extract, and magnolia buds extract is (0.2-0.5):(1-3):(0.2-0.8):(1-3).

3. The application of the soothing and repairing composition containing senna-containing glucosinolates according to claim 1 or 2 in the preparation of skin care products, characterized in that, The skincare products include toners, lotions, creams, masks, and sprays.

4. The application of the soothing and repairing composition containing senna-containing glucosinolates according to claim 1 or 2 in the preparation of products for sensitive skin care, characterized in that, The sensitive skin care products include at least one of toner, lotion, cream, mask, and spray, and the amount of the soothing and repairing composition containing senna glycoside added to the sensitive skin care products is 3%-8%.

5. An emulsion, characterized in that, The emulsion comprises: the soothing and repairing composition containing senna as described in claim 1 or 2, a moisturizer, a thickener, a chelating agent, an emulsifier, an oil, a preservative, a pH adjuster, and a solvent.

6. The emulsion according to claim 5, characterized in that, The method for preparing the emulsion includes the following steps: S1. Mix the humectant, thickener, chelating agent and solvent, heat to 75-85℃, homogenize at 6000-7000 rpm for 4-6 minutes, keep warm after homogenization, and obtain the first mixture. S2. Mix the emulsifier and oil, heat to 75-85℃, and homogenize at 6000-7000 rpm for 4-6 minutes. After homogenization, keep warm for later use to obtain the second mixture. S3. Mix and stir the different preservatives, heat to 55-65℃ until completely dissolved, to obtain the third mixture; S4. Heat the first mixture to 75-85℃, add the second mixture, homogenize at 7500-8500 rpm for 2-4 minutes, then cool to 45-55℃, add the third mixture at 200-400 rpm and mix. Then cool to below 45℃, add the soothing and repairing composition containing senna-based glycosides as described in the first aspect, continue stirring for 4-6 minutes, finally add a pH adjuster to adjust the pH to 5.0-6.5, stop stirring, discharge the material, and obtain the emulsion.

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