An integrated tank for culturing and storing liquid spawn of lentinus edodes

Abstract

The application discloses a kind of culture and store lentinus edodes liquid culture, it is related to the technical field of mycological culture equipment, including jar body, the middle part outer side wall of jar body is equipped with cooling heat exchange cover, and heat transfer bridge is installed between cooling heat exchange cover and the middle part outer side wall of jar body;Upper ultrasonic generator and lower ultrasonic generator are respectively installed in the upper end outer side wall and the lower end outer side wall of jar body;The lower surface edge of jar body is installed with supporting leg, and the inside of the lower surface of jar body is installed with lower liquid outlet;The upper surface of jar body is installed with drive assembly, and drive assembly is transmission connection with stirring assembly;Stirring assembly is installed in the inside of jar body, and the end of stirring assembly is communicated with gas distribution assembly.The application discloses a kind of culture and store lentinus edodes liquid culture, by mechanical stirring and gas stirring and ultrasonic generator, guarantee the sufficiency of mixing, provide low-temperature storage environment, and reduce the link of culture transfer, reduce bacterial contamination.

Description

Technical Field

[0001] This invention relates to the field of fungal cultivation equipment technology, and in particular to an integrated tank for cultivating and storing liquid spawn of shiitake mushrooms. Background Technology

[0002] Shiitake mushrooms are rich in nutrients, have a unique aroma, and possess anti-cancer properties. They are a well-known edible fungus with a long history and are highly favored by consumers both domestically and internationally.

[0003] In terms of the current state of shiitake mushroom production, more than 90% of practitioners still use the long-established solid spawn inoculation technique. However, the new technology of liquid spawn, which is low-cost, easy to operate, has rapid mycelial growth, and high biological conversion rate, is rarely mentioned in the field of shiitake mushroom cultivation.

[0004] Existing liquid culture preparation devices typically use fermenters, which employ either mechanical or gas-driven agitation. The fermenter has a motor at the top, stirring blades inside, and an air inlet valve at the bottom. Agitation is achieved by the motor rotating the stirring blades, disrupting the gas introduced through the air inlet valve, and thus causing the liquid at the bottom of the fermenter to flow. This mechanical agitation method results in a complex structure, high cost, and a relatively high failure rate. Gas agitation, whether using top or bottom air intake, generally suffers from poor aeration and agitation effects. This is especially true in the area of ​​1 / 3 to 3 / 4 of the inner circumference of the lower end of the fermenter, which is a dead zone for gas circulation. This not only results in poor dissolved oxygen but also the presence of culture medium sediment, leading to uneven nutrient distribution in the liquid inside the tank and significantly affecting the quality of the microbial strain. Furthermore, stirring cannot fully and evenly mix the culture medium and the inoculum, making it difficult to clean the inside of the fermenter after cultivation. Furthermore, it is difficult to ensure long-term storage during the storage process, and it is easily affected by miscellaneous bacteria during the transfer process.

[0005] Developing an integrated tank for cultivating and storing liquid shiitake mushroom spawn, using mechanical and gas stirring and an ultrasonic generator to ensure thorough mixing, meet the survival conditions of the spawn, reduce spawn transfer steps, minimize contamination, and further provide a low-temperature storage environment, has become a technical challenge that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0006] The purpose of this invention is to provide an integrated tank for cultivating and storing liquid spawn of shiitake mushrooms, thereby solving the problems listed in the background art.

[0007] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: The present invention provides an integrated tank for culturing and storing liquid shiitake mushroom spawn, comprising a tank body, wherein a cooling heat exchange jacket is fitted on the outer wall of the middle part of the tank body, and a heat transfer bridge is installed between the cooling heat exchange jacket and the outer wall of the middle part of the tank body. An upper ultrasonic generator and a lower ultrasonic generator are respectively installed on the upper outer wall and the lower outer wall of the tank. The lower surface edge of the tank is equipped with support legs, and the lower surface of the tank is equipped with a lower liquid outlet. A drive assembly is installed on the upper surface of the tank, and the drive assembly is connected to the stirring assembly in a transmission manner. The stirring assembly is installed inside the tank, and the end of the stirring assembly is connected to the gas distribution assembly.

[0008] Preferably, an inlet pipe is installed on the upper surface of the tank; A retaining ring is arranged on the outer circumferential surface of the liquid inlet pipe, and a thread is formed on the outer circumferential surface of the retaining ring, which is threadedly connected to the protective cover.

[0009] Preferably, a sensor is installed on the inner side wall of the top of the tank.

[0010] Preferably, the stirring assembly includes a stirring shaft, the lower end of which is fixedly mounted on the inner bottom wall of the tank via a fixed bearing; The upper end of the stirring shaft passes through the upper end of the tank, and a sealed bearing is installed on the outer side wall of the stirring shaft and the upper end of the tank.

[0011] Preferably, telescopic rods are installed at equal intervals on the outer side wall of the stirring shaft, and scrapers are installed at the ends of the telescopic rods, with the scrapers abutting against the inner side wall of the tank.

[0012] Preferably, a stirring rod is also installed on the outer side wall of the stirring shaft, the stirring rod is connected to the stirring shaft, and one-way aeration heads are installed at equal intervals on the outer side wall of the stirring rod.

[0013] Preferably, the drive assembly includes a motor and a gearbox, with the motor fixedly mounted on the upper surface of the tank. The gearbox is fixedly connected to the outer wall of the stirring shaft, and the working end of the motor is connected to the gearbox for transmission.

[0014] Preferably, the gas distribution assembly includes an air intake pipe, one end of which is connected to a gas source, and the other end of which is fitted with a connector, which is connected to the end of the stirring shaft via a turntable.

[0015] Preferably, a filter is also installed on the air intake pipe.

[0016] Compared with the prior art, the beneficial technical effects of the present invention are as follows: This invention discloses an integrated tank for cultivating and storing liquid shiitake mushroom spawn. The spawn and culture medium are mechanically stirred by a stirring shaft driving a telescopic rod, scraper, and stirring rod. At the same time, gas stirring is also performed by a one-way aeration head on the stirring rod. This not only ensures thorough stirring but also prevents sediment from adhering to the inner wall of the tank. It can also be used in conjunction with an ultrasonic generator to ensure sufficient mixing and meet the survival conditions of the spawn. Furthermore, during the sterilization process, high-temperature and high-pressure steam can be introduced through the one-way aeration head for sterilization. At the same time, after the culture is completed, a low-temperature environment is provided by a cooling heat exchange jacket for immediate low-temperature preservation, which not only reduces the transfer of liquid culture but also avoids contamination by other microorganisms. Attached Figure Description

[0017] The present invention will be further described below with reference to the accompanying drawings.

[0018] Figure 1 This is a schematic cross-sectional view of an integrated tank for culturing and storing liquid shiitake mushroom spawn according to the present invention.

[0019] Explanation of reference numerals in the attached drawings: 1. Tank body; 2. Upper ultrasonic generator; 3. Cooling heat exchange jacket; 4. Heat transfer bridge; 5. Scraper; 6. Telescopic rod; 7. One-way aeration head; 8. Stirring rod; 9. Stirring shaft; 10. Fixed bearing; 11. Support leg; 12. Lower liquid outlet; 13. Lower ultrasonic generator; 14. Sealed bearing; 15. Sensor; 16. Motor; 17. Filter; 18. Air inlet pipe; 19. Connector; 20. Turntable; 21. Protective cover; 22. Liquid inlet pipe; 23. Gearbox. Detailed Implementation

[0020] To make the technical problems to be solved, the technical solutions, and the beneficial effects of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0021] like Figure 1 As shown, an integrated tank for cultivating and storing liquid shiitake mushroom spawn includes a tank body 1. A cooling heat exchange jacket 3 is fitted on the outer wall of the middle part of the tank body 1, and a heat transfer bridge 4 is installed between the cooling heat exchange jacket 3 and the outer wall of the middle part of the tank body 1. The liquid spawn inside the tank body is transferred to the middle part of the tank body through the heat transfer bridge. Other parts of the tank body are insulated to prevent heat loss and reduce the power consumption of the cooling heat exchange jacket when storing the liquid spawn inside the tank body at low temperature. The upper outer wall and the lower outer wall of the tank 1 are respectively equipped with an upper ultrasonic generator 2 and a lower ultrasonic generator 13 to fully mix the bacterial strain with the culture medium, thereby improving the survival rate of the bacterial strain. The upper ultrasonic generator 2 and the lower ultrasonic generator 13 are detachably connected to the tank 1. The lower surface edge of the tank body 1 is equipped with support legs 11, and the lower surface of the tank body 1 is equipped with a lower liquid outlet 12. The lower liquid outlet is equipped with a burning ring to prevent contamination by miscellaneous bacteria. A drive assembly is installed on the upper surface of the tank 1, and the drive assembly is connected to the stirring assembly in a transmission manner. The stirring assembly is installed inside the tank 1, and the end of the stirring assembly is connected to the gas distribution assembly.

[0022] Specifically, an inlet pipe 22 is installed on the upper surface of the tank body 1; A retaining ring is arranged on the outer circumferential surface of the liquid inlet pipe 22. The retaining ring has threads on its outer circumferential surface, and the threads are threaded to the protective cover 21. During operation, alcohol is poured into the retaining ring, and the liquid inlet is disinfected by igniting the alcohol, thereby reducing contamination by miscellaneous bacteria.

[0023] Specifically, a sensor 15 is installed on the inner side wall of the top of the tank 1. The sensor can monitor the liquid level and temperature inside the tank and provide data support for the control of the liquid microbial environment inside the tank.

[0024] Specifically, the stirring assembly includes a stirring shaft 9, the lower end of which is fixedly mounted on the bottom inner wall of the tank 1 via a fixed bearing 10; The upper end of the stirring shaft 9 passes through the upper end of the tank body 1, and a sealed bearing 14 is installed on the outer side wall of the stirring shaft 9 and the upper end of the tank body 1.

[0025] Specifically, telescopic rods 6 are installed at equal intervals on the outer side wall of the stirring shaft 9, and scrapers 5 are installed at the ends of the telescopic rods 6. The scrapers 5 abut against the inner side wall of the tank 1, which can effectively prevent sediment from adhering to the inner wall of the tank during the cultivation process.

[0026] Specifically, an agitator 8 is also installed on the outer wall of the agitator shaft 9. The agitator 8 is connected to the agitator shaft 9. One-way aeration heads 7 are installed at equal intervals on the outer wall of the agitator 8 to facilitate the replenishment of gas into the tank. At the same time, high-temperature and high-pressure steam can be introduced into the tank for disinfection during the disinfection process.

[0027] Specifically, the drive assembly includes a motor 16 and a gearbox 23, with the motor 16 fixedly mounted on the upper surface of the tank 1; The gearbox 23 is fixedly connected to the outer wall of the stirring shaft 9, and the working end of the motor 16 is connected to the gearbox 23 for transmission.

[0028] Specifically, the gas distribution assembly includes an air inlet pipe 18, one end of which is connected to a gas source, and the other end of which is equipped with a connector 19. The connector 19 is connected to the end of the stirring shaft 9 via a turntable 20. Furthermore, the end of the stirring shaft is equipped with a plug, and the connector can be detached from the end of the stirring shaft after the gas distribution operation is completed.

[0029] Specifically, a filter 17 is also installed on the air inlet pipe 18. The filter can filter and disinfect during the air circulation process to prevent bacteria from contaminating the liquid inside the tank during the air circulation process.

[0030] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0031] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. An integrated tank for culturing and storing Lentinula edodes liquid culture, characterized by: Includes a tank (1), on which a cooling heat exchange jacket (3) is fitted on the outer wall of the middle part of the tank (1), and a heat transfer bridge (4) is installed between the cooling heat exchange jacket (3) and the outer wall of the middle part of the tank (1). The upper outer wall and the lower outer wall of the tank (1) are respectively equipped with an upper ultrasonic generator (2) and a lower ultrasonic generator (13). The lower surface edge of the tank (1) is equipped with a support leg (11), and the lower surface of the tank (1) is equipped with a lower liquid outlet (12). A drive assembly is installed on the upper surface of the tank (1), and the drive assembly is connected to the stirring assembly in a transmission manner; The stirring assembly is installed inside the tank (1), and the end of the stirring assembly is connected to the gas distribution assembly.

2. The integrated tank for cultivating and storing liquid shiitake mushroom spawn according to claim 1, characterized in that: The upper surface of the tank (1) is equipped with a liquid inlet pipe (22). A retaining ring is arranged on the outer circumferential surface of the liquid inlet pipe (22), and a thread is provided on the outer circumferential surface of the retaining ring, which is threadedly connected to the protective cover (21).

3. The integrated tank for cultivating and storing liquid shiitake mushroom spawn according to claim 1, characterized in that: A sensor (15) is installed on the inner side wall of the top of the tank (1).

4. The integrated tank for cultivating and storing liquid shiitake mushroom spawn according to claim 1, characterized in that: The stirring assembly includes a stirring shaft (9), the lower end of which is fixedly mounted on the bottom inner wall of the tank (1) by a fixed bearing (10). The upper end of the stirring shaft (9) passes through the upper end of the tank (1), and a sealed bearing (14) is installed on the outer side wall of the stirring shaft (9) and the upper end of the tank (1).

5. The integrated tank for cultivating and storing liquid shiitake mushroom spawn according to claim 4, characterized in that: Telescopic rods (6) are installed at equal intervals on the outer side wall of the stirring shaft (9), and scrapers (5) are installed at the ends of the telescopic rods (6). The scrapers (5) abut against the inner side wall of the tank (1).

6. The integrated tank for cultivating and storing liquid shiitake mushroom spawn according to claim 4, characterized in that: A stirring rod (8) is also installed on the outer wall of the stirring shaft (9). The stirring rod (8) is connected to the stirring shaft (9). One-way aeration heads (7) are installed at equal intervals on the outer wall of the stirring rod (8).

7. The integrated tank for cultivating and storing liquid shiitake mushroom spawn according to claim 6, characterized in that: The drive assembly includes a motor (16) and a gearbox (23), wherein the motor (16) is fixedly mounted on the upper surface of the tank (1); The gearbox (23) is fixedly connected to the outer wall of the stirring shaft (9), and the working end of the motor (16) is connected to the gearbox (23) for transmission.

8. The integrated tank for cultivating and storing liquid shiitake mushroom spawn according to claim 7, characterized in that: The gas distribution assembly includes an air inlet pipe (18), one end of which is connected to a gas source, and the other end of which is fitted with a connector (19). The connector (19) is connected to the end of the stirring shaft (9) via a turntable (20).

9. The integrated tank for culturing and storing liquid shiitake mushroom spawn according to claim 8, characterized in that: A filter (17) is also installed on the air intake pipe (18).

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