A hybridization breeding method for nymphaea lotus
By using hybridization breeding methods between Minuta water lilies and tropical water lily varieties, the breeding problem of the sepal variant crab claw water lily has been solved, improving its ornamental and economic value and filling a gap in the variety.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
- Filing Date
- 2026-04-29
- Publication Date
- 2026-06-09
AI Technical Summary
my country lacks novel and distinctive high-quality varieties of water lilies, especially the crab claw water lily with sepal mutations, which is difficult to cultivate stably through targeted hybridization, resulting in its ornamental value not being fully realized.
Using *Nymphaea minuta* as the female parent and combining it with *Nymphaea glomeratus* or other tropical water lily varieties as the male parent, a new variety of *Nymphaea crape myrtle* with sepal dissimilarity was obtained through methods such as bagging isolation, mixed pollination, and seed sowing, ensuring the operability and reproducibility of the breeding.
A new variety of crab claw water lily with sepal mutation and significant ornamental value has been successfully cultivated, filling a gap in domestic varieties and possessing good economic value and application prospects.
Smart Images
Figure CN122162701A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of water lily hybridization breeding technology, specifically relating to a hybridization breeding method for obtaining crab claw water lilies. Background Technology
[0002] Water lilies (Nymphaea spp.) are perennial aquatic plants belonging to the genus Nymphaea in the family Nymphaeaceae. They have large, beautiful flowers with vibrant colors and graceful forms, making them a prized aquatic flower. Known as the "Sleeping Beauty of Flowers," they are an indispensable element in garden waterscapes and possess high cultural, ornamental, and practical value.
[0003] Currently, my country still faces shortcomings in the research and production of water lilies, especially in the lack of novel and distinctive high-quality self-bred varieties. With increasing awareness of germplasm resource protection abroad, the introduction of superior varieties is becoming increasingly difficult, thus urgently requiring the acceleration of variety selection and breeding work for tropical water lilies in my country. Some varieties with high ornamental value include the sepal-altered water lily, commonly known as the "crab claw water lily," where the normal four sepals are replaced by eight. Each sepal consists of a flattened stalk and triangular groove-shaped leaves. Two of these are larger, resembling crab claws, while the other six are smaller, resembling crab legs. The central flower resembles the crab's body. When in full bloom, it resembles a crawling crab. The outer petals are also sepal-like, green on the back and deep blue-purple on the front. The flower can open normally. However, after the petals, stamens, and pistils inside the flower die and rot, the eight altered sepals continue to grow and function as leaves. This mutation has unique ornamental value, satisfying the needs of crab claw enthusiasts. However, these varieties are natural mutations. Therefore, if a varietal mutation crab claw water lily variety can be stably bred through hybridization, it will be of great significance to the development of the water lily industry. Summary of the Invention
[0004] In view of the shortcomings of existing technology, in order to cultivate new varieties of crab claw water lilies with sepal mutations, the inventors, after years of self-pollination breeding and hybridization combination experiments, finally discovered that using Minuta water lily as the female parent and other tropical water lilies as the male parent can stably mutate into new varieties of crab claw water lilies. The experiment is operable and reproducible, and is worth promoting and applying.
[0005] To achieve the above objectives, the present invention adopts the following technical solution: A hybridization breeding method for a sepal-dissimilated crab claw water lily includes the following steps: using Minuta water lily ( Nymphaea minuta ) as the parent plant, with the tropical water lily Minuta water lily ( Nymphaea minuta Tropical sunset water lilies Nymphaea. ' Tropical Sunset ), Kara Sunshine Water Lily ( Nymphaea. ' Carla's Sonshine Using at least one of the following as the male parent, self-pollination or hybridization is carried out to obtain sepal-dissimilar water lily (Crab Claw Water Lily).
[0006] Preferably, the hybridization breeding method includes the following steps: A. Before the flowers of the male and female parents open, bag them for isolation to avoid contamination by non-target pollen and its impact on breeding results.
[0007] B. Take the stigma extract from the mother plant; C. Collect pollen from the male parent, mix the pollen with the stigma liquid from the female parent, and pollinate after mixing evenly; when pollinating, use a pipette to dry the stigma liquid from the female parent's pistil and immediately add pollination solution; after pollination, continue to isolate by bagging. D. After pollination, harvest the fruit and obtain the seeds.
[0008] Preferably, in step A, the flowers are bagged and isolated one day before they open.
[0009] Preferably, in step B, the stigma extract is collected on the day the mother plant flowers.
[0010] Preferably, the mass ratio of pollen to stigma extract of the parent plant is 1:3~5.
[0011] Preferably, the obtained seeds are sown and screened to obtain different colored sepal-dissimilar water lilies.
[0012] As a preferred option, self-pollination was performed using *Nymphaea minuta* as the female and male parent to obtain *Nymphaea crape myrtle* with pure white sepals.
[0013] As a preferred option, hybridization was carried out using *Nymphaea minuta* as the female parent and *Nymphaea tropical sunset* as the male parent to obtain *Nymphaea crape-shaped* with pink sepals.
[0014] As a preferred option, hybridization was carried out using *Nymphaea minuta* as the female parent and *Nymphaea cara* as the male parent to obtain *Nymphaea crape myrtle* with yellow sepals.
[0015] The beneficial effects of this invention are: This invention provides a hybridization breeding method for a sepal-mutated crab claw water lily, which has bred a new variety of crab claw water lily with sepal mutation. This new variety has significant differences from its parent varieties in terms of sepals, flower color, and leaf color, and has high ornamental value. It can fill the gap in my country's new varieties of crab claw water lilies, has good economic value, and is worthy of promotion and application. Attached Figure Description
[0016] Figures 1-3 This invention pertains to the hybridization breeding process. Specifically, Figure 1 It is a hybrid of the Minuta water lily and the tropical sunset water lily; Figure 2 It is a hybrid of Minuta water lily and Cara Sunshine water lily; Figure 3 For Minu, the water lily self-pollinates. Detailed Implementation
[0017] To facilitate a clearer understanding of the technical content of this invention by those skilled in the art, the invention will be further described below in conjunction with specific embodiments and accompanying drawings.
[0018] Unless otherwise specified, the experimental methods described in the following examples are conventional methods.
[0019] Experimental example: Breeding creation of crab claw water lily with sepal variation The specific breeding process is as follows: (1) Determine breeding objectives The goal of the breeding program is to create new varieties of *Water Lily simonii* with differentiated sepals.
[0020] (2) Selection of parents Parent: Minuta scientific name: N.minuta Characteristics: Pale pink flowers, oval green leaves.
[0021] Habits: Prefers light and heat, dislikes cold.
[0022] Reproduction: Seed reproduction.
[0023] It belongs to the tropical water lily family, has a strong fruit-bearing ability, and is a very good mother plant for water lilies.
[0024] The paternal parent plants include water lilies such as Minuta, Tropical Sunset, and Kara Sunshine. The characteristics of the paternal parent water lilies are as follows: A. Tropical Sunset Water Lily scientific name: N. ' Tropical Sunset Characteristics: Pollen is yellow and multicolored, bowl-shaped, emergent, and leaves have dark patches.
[0025] Habits: Prefers strong light and heat.
[0026] Reproduction: Viviparous reproduction.
[0027] B. Kara Sunshine Water Lily scientific name: N. ' Carla's Sonshine Characteristics: Golden yellow flowers, emerges from water, oval leaves with brownish-red patches.
[0028] Habits: Prefers strong light and heat.
[0029] Reproduction: Viviparous reproduction.
[0030] C. Minuta Water Lily scientific name: N.minuta Characteristics: Miniature variety, strong fruit-bearing ability, green and oval leaves, white pollen.
[0031] Habits: Prefers light and heat, but is not cold-resistant.
[0032] Reproduction: Seed reproduction.
[0033] (3) Hybridization and breeding process (A) Bagging and isolating the male and female plants one day before their flowers open; (B) Collect stigma extract on the morning of the day the mother plant flowers.
[0034] (C) Collect pollen from the male parent that opened on the second day, mix the pollen with the stigma liquid of the female parent (mass ratio 1:5), and pollinate after thorough mixing. During pollination, use a pipette to dry the stigma liquid of the female parent's pistil and immediately add the pollination solution. After pollination, continue to isolate the flowers by bagging them. A total of no less than 1,000 flowers should be pollinated.
[0035] (D) Harvest the fruit about 30 days after pollination, obtain the seeds, and store them in water for later use.
[0036] (E) Seeds sown and harvested under suitable temperature and season yielded offspring plants. After flowering, the plants were investigated for their traits. The results are as follows: Table 1
[0037] The results showed that only offspring from hybridization of *Nymphaea minuta* as the female parent and *Nymphaea tropical sunset*, *Nymphaea carambola*, and *Nymphaea minuta* as the male parents could produce *Nymphaea crassifolia* with sepal dissimilarity. Repeated experiments verified that the proportion of *Nymphaea crassifolia* with sepal dissimilarity obtained in each hybridization breeding could reach more than one in ten thousand.
[0038] Example 1: A hybridization breeding method for a crab claw water lily with sepal dissimilarity The parent plant was the Minuta water lily (Minota water lily) and the parent plant was the tropical sunset water lily (Tropical sunset water lily). A. Isolate the male and female plants by bagging them one day before the flowers open; B. Collect stigma extract on the morning of the day the mother plant flowers; C. Collect pollen from the male parent that opened on the second day, and mix the pollen with the stigma liquid from the female parent (mass ratio 1:3). After mixing thoroughly, perform pollination. During pollination, use a pipette to dry the stigma liquid from the female parent's pistil and immediately add the pollination solution. After pollination, continue to isolate the plant by bagging it.
[0039] D. About 30 days after pollination, harvest the fruit, obtain the seeds, and store them in water for later use.
[0040] E. Seeds harvested at suitable temperatures and seasons were sown, and after the plants flowered, pheasant characteristics were investigated to obtain a new variety of crab claw water lily with differentiated sepals.
[0041] Example 2: A breeding method for a crab claw water lily with sepal dissimilarity Self-fertilization was carried out using Minuta as the female parent and Minuta as the male parent. A. Isolate the male and female plants by bagging them one day before the flowers open; B. Collect stigma extract on the morning of the day the mother plant flowers; C. Collect pollen from the male parent that opened on the second day, and mix the pollen with the stigma liquid from the female parent (mass ratio 1:3). After mixing thoroughly, perform pollination. During pollination, use a pipette to dry the stigma liquid from the female parent's pistil and immediately add the pollination solution. After pollination, continue to isolate the plant by bagging it.
[0042] D. About 30 days after pollination, harvest the fruit, obtain the seeds, and store them in water for later use.
[0043] E. Seeds harvested at suitable temperatures and seasons were sown, and after the plants flowered, pheasant characteristics were investigated to obtain a new variety of crab claw water lily with differentiated sepals.
[0044] Example 3: A hybridization breeding method for a crab claw water lily with sepal dissimilarity The parent plant was the Minuta water lily (Miniata water lily) and the Kara Sunshine water lily (Kara Sunshine water lily). A. Isolate the male and female plants by bagging them one day before the flowers open; B. Collect stigma extract on the morning of the day the mother plant flowers; C. Collect pollen from the male parent that opened on the second day, and mix the pollen with the stigma liquid from the female parent (mass ratio 1:3). After mixing thoroughly, perform pollination. During pollination, use a pipette to dry the stigma liquid from the female parent's pistil and immediately add the pollination solution. After pollination, continue to isolate the plant by bagging it.
[0045] D. About 30 days after pollination, harvest the fruit, obtain the seeds, and store them in water for later use.
[0046] E. Seeds harvested at suitable temperatures and seasons were sown, and after the plants flowered, pheasant characteristics were investigated to obtain a new variety of crab claw water lily with differentiated sepals.
[0047] In other embodiments of the present invention, other tropical strains may also be selected as the parent.
[0048] The above description is only a partial embodiment of the technical solution of the present invention, intended to help those skilled in the art understand the core concept of the present invention, and is not an exhaustive list of all embodiments of the present invention. The scope of protection of the present invention shall be determined by the claims and their equivalents. Any equivalent substitutions, further optimizations and improvements to the technical features implemented based on the basic principles and concepts disclosed in the present invention shall fall within the scope of protection of the present invention.
Claims
1. A hybridization breeding method for a sepal-dissimilarity *Water Lily*, characterized in that, The hybridization breeding method includes the following steps: using Minuta water lily (… Nymphaea minuta Using 1000 spp. as the female parent and 1000 spp. as the male parent, self-pollination or hybridization was carried out to obtain the sepal-dissimilar crab claw water lily.
2. The hybridization breeding method for a sepal-dissimilarity *Nelumbo nucifera* according to claim 1, characterized in that, The paternal parent is *Minota water lily* ( Nymphaea minuta Tropical sunset water lilies Nymphaea. ' Tropical Sunset ), Kara Sunshine Water Lily ( Nymphaea. ' Carla's Sonshine At least one of the following.
3. The hybridization breeding method for a sepal-dissimilarity *Nelumbo nucifera* according to claim 1, characterized in that, The hybridization breeding method includes the following steps: A. Before the flowers of the male and female parents open, isolate them by bagging; B. Take the stigma extract from the mother plant; C. Collect pollen from the male parent, mix the pollen with the stigma liquid from the female parent, and pollinate after mixing evenly; when pollinating, use a pipette to dry the stigma liquid from the female parent's pistil and immediately add pollination solution; after pollination, continue to isolate by bagging. D. After pollination, harvest the fruit and obtain the seeds.
4. The hybridization breeding method for a sepal-dissimilarity *Nelumbo nucifera* according to claim 3, characterized in that, In step A, the flowers are isolated by bagging them the day before they open.
5. The hybridization breeding method for a sepal-dissimilarity *Nelumbo nucifera* according to claim 3, characterized in that, In step B, the stigma extract is collected on the morning of the day the mother plant flowers.
6. The hybridization breeding method for a sepal-dissimilarity *Nelumbo nucifera* according to claim 3, characterized in that, The mass ratio of pollen to stigma extract from the parent plant is 1:3~5.
7. The hybridization breeding method for a sepal-dissimilarity *Nelumbo nucifera* according to claim 3, characterized in that, The obtained seeds were sown, and different colored sepal-dissimilar water lilies were selected.
8. The hybridization breeding method for a sepal-dissimilarity *Nelumbo nucifera* according to claim 1, characterized in that, Self-pollination using *Nymphaea minuta* as the female and male parent yielded *Nymphaea crape myrtle*, a variant with pure white sepals.
9. A hybridization breeding method for a sepal-dissimilarity *Nelumbo nucifera* according to claim 1, characterized in that, By hybridizing *Nymphaea minuta* as the female parent and *Nymphaea tropical sunset* as the male parent, a pink-flowered sepal-dissimilar *Cranium spp.* was obtained.
10. A hybridization breeding method for a sepal-dissimilated *Nelumbo nucifera* according to claim 1, characterized in that, By hybridizing *Nymphaea minuta* as the female parent and *Nymphaea cara* as the male parent, a hybridized *Nymphaea crape myrtle* with yellow sepals was obtained.