Method for indoor breeding of anisakidae which preys on termites
By using a four-stage indoor rearing method and specialized feed, the problems of breeding difficulties, high cannibalism rate, and domestication of the worm have been solved, achieving efficient and stable natural enemy population cultivation and providing a reliable technical solution for its application in the field.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- HUAZHONG AGRI UNIV
- Filing Date
- 2026-04-02
- Publication Date
- 2026-06-09
AI Technical Summary
Existing technologies make it difficult to achieve large-scale artificial breeding of the worm. The egg hatching rate is low, the cannibalism rate is high, the control efficacy cannot be guaranteed, and the special feed lacks specific attractants and nutritional factors, which leads to the domestication of natural enemies.
A four-stage indoor rearing method is adopted, including independent nest building and breeding, mother-infant co-rearing, separate rearing within the same litter, and maintenance of homologous populations. Combined with special artificial feed, the biomimetic environment is provided to induce natural reproductive behavior and inhibit intraspecific cannibalism by utilizing kinship recognition characteristics and specific nutritional supply.
It significantly improved the egg hatching rate and the survival rate of nymphs to adults, maintained a high predatory desire for target pests, and formed an efficient and stable natural enemy population, laying the foundation for industrial production.
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Figure CN122162756A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of artificial breeding technology of natural enemy insects, and in particular relates to an indoor rearing method for termite predators. Background Technology
[0002] Black-winged subterranean termites (Odontotermes formosanus) are major pests damaging embankments, garden trees, and dryland crops, and both chemical and physical control methods have limitations. The invasive termite beetle (Odontotermes formosanus), a newly discovered and highly efficient natural enemy capable of invading termite nests and preying, shows great promise. However, large-scale artificial rearing of this beetle faces a series of biological challenges. Female invasive termites exhibit strong maternal care and soil-building habits, making them highly susceptible to disturbance and egg-eating in artificial rearing environments, resulting in extremely low hatching rates and becoming the primary obstacle to large-scale breeding. Furthermore, the intense intraspecific cannibalism during both nymphal and adult stages means that using conventional insect-based mixed rearing methods will lead to a sharp decline in survival rates, hindering economically efficient population expansion. Finally, patent CN112586637A discloses an artificial feed for earwigs. The artificial feed for earwigs is prepared with a formula mainly composed of beans, yeast, etc. Although it can maintain basic survival, it lacks the specific feeding and nutritional factors necessary to maintain the earwig's high predatory desire for black-winged subterranean termites. Long-term feeding can easily lead to the "domestication" of natural enemies and a decline in field control capabilities.
[0003] In existing technologies, the rearing of earwigs mainly focuses on providing physical isolation to reduce cannibalism (such as setting up strip-shaped barriers) or optimizing general feed formulations to promote growth. However, no systematic design has been made for the underground activity habits, complex social behaviors, and nutritional relationships with specific prey of the earwig, a specific species.
[0004] Therefore, developing a proprietary breeding method that can simultaneously solve the problems of difficult reproduction, high losses, and declining effectiveness is a key technical issue that urgently needs to be addressed to promote the transformation of this highly efficient natural enemy from the laboratory to practical engineering applications. Summary of the Invention
[0005] To address the aforementioned problems, this invention provides an indoor rearing method for termite predators, which aims to induce their natural reproductive behavior through a biomimetic environment, utilize their kinship recognition characteristics to build low-conflict communities, and maintain their predation targeting through specific feed. This systematically solves the problems of low reproduction rate, high cannibalism rate, and inability to guarantee control efficacy in existing technologies.
[0006] To achieve the above-mentioned objectives, the present invention adopts the following technical solution: An indoor rearing method for termite-preying worms includes the following stages: independent nest building and breeding stage, mother-infant co-rearing stage, same-nest group rearing stage, and same-origin population maintenance stage, and special artificial feed is provided in the above four stages.
[0007] Furthermore, by weight percentage, the special artificial feed comprises the following components: 25% black-winged termite powder, 20% mealworm powder, 35% carrot pulp, 0.1% potassium sorbate, 1% compound vitamin and mineral premix, and the remainder is water.
[0008] The black-winged termite powder in the special artificial feed provided by this invention provides species-specific feeding signals and key nutritional components.
[0009] Furthermore, the specific operation of the independent nesting and breeding stage is as follows: the mated male and female adult worms are placed in an independent breeding container with soil substrate at the bottom according to the pairing method, and raised in a quiet and dark environment. After the female worm digs a nest in the soil substrate and lays eggs, the male worm is removed.
[0010] Furthermore, the thickness of the soil substrate is 2-3 cm, and the moisture content is 20-25%; during the independent nesting and breeding stage, the temperature is controlled at 25±1℃ and the relative humidity is 85±5%.
[0011] Furthermore, the independent rearing container is an opaque container, and the environment is kept dark and free from vibration during the period when the female insect protects the eggs and the eggs hatch.
[0012] The independent nesting and breeding stage of this invention provides each mated pair of adults with an independent rearing container, in which a 2-3 cm thick layer of moist, sterile soil substrate is laid. The insects are reared in a quiet, dark environment at 25±1℃ and 85±5% relative humidity, inducing the females to autonomously dig nests and lay eggs in the soil substrate. After confirming egg laying, the males are removed. By maintaining a stable, undisturbed environment, egg-eating behavior during the egg-guarding period is effectively avoided.
[0013] Furthermore, the specific operation of the mother-infant co-nursing stage is as follows: maintain the same rearing conditions in the independent rearing box as in the independent nesting and breeding stage, wait for the eggs to hatch naturally in the nest, and for the female insect to live together with the hatched nymphs.
[0014] Furthermore, the period of cohabitation is 30 days.
[0015] In the mother-infant co-nursing stage set by this invention, after the eggs hatch in a safe nest, the female and nymphs continue to coexist in the original container for 30 days. During this stage, they are fed with special artificial feed while maintaining soil moisture, making full use of the female's natural larval care behavior, which greatly improves the survival rate and uniformity of early nymph growth.
[0016] Furthermore, the specific operation of the same-nest group rearing stage is as follows: transfer the entire brood of nymphs hatched in the same nest during the mother-infant co-rearing stage to an independent rearing box, remove the female nymphs, maintain the same rearing conditions as the mother-infant co-rearing stage, and continue to rear the nymphs until they emerge as adults.
[0017] Furthermore, during the transfer process, the entire nymphal colony and part of the original nest soil from the mother-infant co-rearing stage are transferred to individual rearing boxes; the rearing density during the in-brood group rearing stage is 5-8 individuals / 100cm². 2 .
[0018] This invention involves setting up a brood separation rearing stage, transferring an entire brood of nymphs, along with some of the original nest soil, to a larger rearing container, after which the female is removed. Utilizing the kinship recognition characteristics among individuals within the same brood of *Eriocaulon buxiflora*, they are reared in groups within this new container, which can be lined with moist soil to provide shelter for the nymphs and achieve natural separation.
[0019] Furthermore, the specific operation of the homologous population maintenance stage is as follows: adults from the same nest are raised together as a homologous population unit in a final rearing container, maintaining the same rearing conditions as the mother-infant co-rearing stage.
[0020] This invention treats adult insects from the same brood as a stable social unit, and raises them together in a final rearing container as a high-quality breeding population or a reserve for field release.
[0021] Furthermore, the homologous population maintenance stage also includes placing end-care containers carrying different homologous population units into a lifetime rearing unit collection box. This lifetime rearing unit collection box has unified ventilation channels and centralized feed and water replenishment points. The lifetime rearing unit collection box integrates multiple independent end-care containers into a unified management system, significantly improving the management efficiency and stability of large-scale population maintenance and reducing labor costs by providing a stable microenvironment and convenient centralized feeding and observation.
[0022] Compared with the prior art, the present invention has the following advantages and technical effects: 1. Efficiently solves the breeding problem: The indoor rearing method of termite-preying worms provided by this invention successfully induces and stabilizes the natural nest-building and egg-protecting behavior of female insects by providing key soil substrate and quiet and dark environment. This fundamentally overcomes the problem of egg-eating caused by disturbance in artificial rearing, significantly improves the egg hatching rate, and breaks through the core bottleneck of large-scale propagation.
[0023] 2. Effectively suppress intraspecific cannibalism: The indoor rearing method of *Ceratophyllum demersum*, which preys on termites, provided by this invention abandons the passive approach of relying solely on physical isolation. It innovatively utilizes the brood affinity behavior of *Ceratophyllum demersum* to implement community management, effectively suppressing intraspecific cannibalism in large-scale rearing and significantly improving the overall survival rate of nymphs to adults.
[0024] 3. Excellent population quality: The indoor rearing method of termite-preying worms provided by this invention ensures that the natural enemies maintain a high predatory desire and recognition ability for the target pests by feeding them a special artificial feed with black-winged subterranean termite powder as the core throughout the entire rearing period. The key indicators of the predatory ability of the bred worm adults against black-winged subterranean termites are significantly better than those of individuals fed with general feed.
[0025] 4. Formation of a complete technical system: This invention organically combines biomimetic environmental design (soil substrate), behavioral stage management (four-stage process), and specific nutrient supply (special feed), and for the first time establishes a closed-loop, standardized breeding system for the specialized natural enemy of earwigs, from reproduction and growth to performance maintenance, laying a solid foundation for its factory production and field application, which is significantly superior to the existing general earwig breeding technology.
[0026] 5. Facilitates large-scale integrated management: This invention introduces modular lifelong rearing unit collection boxes to centrally maintain homologous populations, providing a key equipment interface for scaling up the indoor rearing method of termite predators from laboratory scale to industrial production, significantly improving management efficiency, environmental uniformity, and population quality stability in the later stages of large-scale rearing. Attached Figure Description
[0027] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0028] Figure 1 This is a schematic diagram illustrating the characteristics of a symbiotic earwig, where A represents nest building, B represents egg laying, C represents egg protection, and D represents juvenile care. Figure 2 A diagram illustrating the feeding behavior of a mangrove larvae; Figure 3 Photograph of a cascade enclosure for lifelong rearing of *Ceratophyllum demersum* used to maintain homologous populations. Detailed Implementation
[0029] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.
[0030] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.
[0031] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.
[0032] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.
[0033] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.
[0034] This invention provides an indoor rearing method for termite-preying subterranean termites, comprising four sequential stages: independent nest building and breeding, mother-infant co-rearing, group rearing within the same nest, and maintaining a homologous population. In the independent nest building and breeding stage, by providing a moist, sterile soil substrate for paired adults and inducing females to autonomously dig nests and lay eggs in a quiet, dark environment, the problem of disturbed egg-eating that easily occurs in artificial rearing is effectively overcome. By implementing a strategy of transferring and group rearing nymphs from the same nest, intraspecific cannibalism is significantly suppressed. Throughout the process, the insects are fed a specialized artificial feed with black-winged subterranean termites as the core component (25% freeze-dried black-winged termite powder, 20% mealworm powder, 35% carrot pulp, 0.1% potassium sorbate, 1% commercial insect compound vitamin and mineral premix, with the remainder being water).
[0035] Tests showed that the indoor rearing method for predatory termite-eating earwigs provided by this invention achieved an egg hatching rate of 95.33%, a nymph-to-adult survival rate of 82.77%, and an average adult weight of 192.23 mg. The instantaneous attack rate (0.972) of the bred adults against black-winged subterranean termites was significantly improved, with a theoretical maximum daily predation rate of 500 termites, and a pest control efficacy (486,000) far superior to the comparative method. Compared with existing earwig rearing techniques, this invention improves the egg hatching rate and nymph-to-adult survival rate, significantly reduces intraspecific cannibalism losses, and the bred adults exhibit stronger aggression against black-winged subterranean termites. This invention achieves efficient, stable, and large-scale breeding of earwigs, providing a reliable technical solution for their application as specialized natural enemies in the green control of termites in dikes.
[0036] The room temperature in this invention refers to 25±2℃.
[0037] The feed preparation raw materials used in this invention are obtained through commercial purchase.
[0038] The technical means by which the collection box simultaneously monitors the status and maintains the environment of dozens of homologous population units in this embodiment of the invention is a conventional technical means, and will not be further elaborated in this invention.
[0039] Example 1: Preparation method of special artificial feed Weigh the following ingredients by weight percentage: 25% freeze-dried black-winged termite powder, 20% mealworm powder, 35% carrot pulp (made by blending fresh carrots with a small amount of water, with the water content included in the total water content), 0.1% potassium sorbate, 1% commercial insect compound vitamin and mineral premix, and 18.9% purified water. To prepare, first dissolve the potassium sorbate in a portion of hot water, then add all other ingredients and stir thoroughly to obtain the specialized artificial feed. Store at 4℃.
[0040] Example 2: An indoor rearing method for termite-preying crickets (1) Independent nest building and breeding stage: Prepare opaque individual rearing boxes (15cm×10cm×10cm). Line the boxes with a 2.5cm thick layer of moist, sterile soil substrate, sterilized by autoclaving and with a moisture content adjusted to 20-25%. Select healthy, mated adult *Ceratophyllum demersum*, pairing one male and one female and placing them in the boxes. Place the individual rearing boxes in a climate chamber with precise temperature and humidity control. Set the culture conditions to: temperature 25±0.5℃, relative humidity 85±3%, and complete darkness. Place the climate chamber in a quiet, vibration-proof location. Under suitable conditions, the female will autonomously dig a nest and lay eggs (e.g., ...). Figure 1As shown in Figures A and B), after confirming egg-laying, the male insects were removed under extremely weak red light. The environment was then strictly kept dark and vibration-free until hatching, during which time the insects were fed the special artificial feed prepared in Example 1.
[0041] (2) Mother-infant co-nursing stage: Maintaining the environmental conditions of step (1) (temperature 25±0.5℃, relative humidity 85±3%, no light, no vibration), the eggs will hatch naturally in the nest after about 15-20 days, and the female insect will guard the nest (e.g., Figure 1 (As shown in C and D), newly hatched nymphs gather and become active, and begin to be fed the special artificial feed prepared in Example 1, such as... Figure 2 As shown. To facilitate feeding of nymphs, the feed can be made into small pellets, and a small amount of feed can be placed near the entrance of the nest. During this period, the soil moisture (20-25%) can be maintained by regularly spraying the walls of the individual rearing box.
[0042] (3) Stage of separate rearing within the same litter: After approximately 30 days of mother-infant co-rearing, the entire nymph colony, along with some soil from its original nest, was carefully transferred to a larger, independent rearing box (23.5cm × 32.5cm × 8cm). A thin layer of moist, sterile soil substrate (2-3cm thick, 20-25% moisture content) was placed at the bottom of the new rearing box for hiding. The female was removed, and the environmental conditions remained unchanged (temperature 25±0.5℃, relative humidity 85±3%, no light, no vibration). The nymphs were fed regularly in block form of the specialized feed prepared in Example 1, with a rearing density of 5-8 nymphs / 100cm². 2 They are raised under these conditions until they emerge as adults.
[0043] (4) Homologous population maintenance stage: Adult insects from the same brood were collectively reared as a homologous population unit in a final rearing container. The rearing environment conditions in the final rearing container were as described above, and the insects were fed the special feed prepared in Example 1 at regular intervals. As a lifelong rearing unit, this unit exhibited good compatibility among individuals and minimal fighting, making it suitable for use as a breeding population or for field release reserves.
[0044] (5) Centralized maintenance: To achieve efficient and intensive management, multiple life-long rearing boxes carrying different homologous population units are placed into a dedicated life-long rearing unit collection box (e.g., Figure 3The entire rearing unit (as shown) is centrally maintained within a single enclosure. This enclosure is equipped with a unified ventilation system and centralized feed and water supply points. During this period, the unit is fed regularly with the specialized feed prepared in Example 1. The enclosure is a modified version of a commercially available modular rearing rack or similar equipment, with independent compartments for the final rearing boxes. This enclosure can simultaneously monitor the status and maintain the environment of dozens of homologous population units, significantly improving the efficiency and reliability of management in the later stages of mass rearing, and ensuring the uniformity and health of the resulting predator population.
[0045] During the five rearing stages, the feeding amount was dynamically adjusted according to the developmental needs and feeding feedback of each stage: 0.2-0.3g per pair per feeding during the independent nesting stage; 0.2g per box per feeding during the mother-infant co-rearing stage; 0.8-2.0g per box per feeding during the same-nest grouping stage; and 2.0-2.5g per box per feeding during the homologous population maintenance stage. Feeding was carried out on average once every 2-3 days and adjusted according to the remaining amount.
[0046] Comparative Example 1: Simulation of existing common earwig rearing methods The insects were reared according to the artificial feed and group rearing method described in Example 1 of patent CN112586637 A. In this method, rearing boxes of the same size were used, but instead of soil, a mixture of peat moss and sandy loam (weight ratio 10:20) was laid as bedding in Example 1 of patent CN112586637 A, and multiple strips of pleated paper were placed as isolation barriers. Male and female adult *Eriocaulon gracilis* were randomly mixed and reared in groups (without pairing or separating them into boxes), at a density of 5-8 insects / 100 cm². 2 The rearing environment was set at 30℃ and 70%RH, with a light-dark cycle of 10h:14h. During this period, the artificial feed and the bedding mixture of peat moss and sandy loam from Example 1 were replaced regularly. No staged management was carried out, and eggs, nymphs, and adults were reared together.
[0047] Comparative Example 2: Differences in Simulated Feeding Methods This comparative example is the same as Comparative Example 1, except that the artificial feed in Comparative Example 1 is replaced with the special artificial feed prepared in Example 1.
[0048] Comparative Example 3: Simulated Feed Differences Same as Example 2, except that the special artificial feed in Example 1 is replaced with the artificial feed in Comparative Example 1.
[0049] Results analysis: The growth and development indicators of *Cypripedium spp.* in Example 2 and Comparative Examples 1-3 were investigated and measured, and the results are shown in Table 1.
[0050] Table 1. Growth and development indicators of *Cypripedium spp.* under different feeding conditions Table 1 shows that the reproductive capacity of *Ceratophyllum demersum* was significantly improved in Example 2. The egg hatching rate of *Ceratophyllum demersum* raised in Example 2 reached 95.33%, significantly higher than that of Comparative Example 2 (59.20%). Meanwhile, the egg-eating rate during the egg-guarding period was only 3.14%, significantly lower than that of Comparative Example 1 (25.92%) and Comparative Example 2 (37.26%). This indicates that while using the special artificial feed prepared in Example 1 (Comparative Example 2) can partially improve the reproductive environment, it cannot effectively suppress the stress-induced egg-eating behavior of female insects caused by improper feeding management. This invention, by providing a moist, sterile soil substrate and a quiet, undisturbed biomimetic environment, successfully induced female insects to complete natural nest building and egg-guarding behaviors, thus fundamentally solving a key obstacle in artificial breeding.
[0051] Table 1 shows that *Cypripedium spp.* in Example 2 exhibited a significant advantage in population survival and growth. During the nymph-to-adult development stage, the nymph-to-adult survival rate of Example 2 was the highest (82.77%), and the mortality rate due to intraspecific cannibalism was the lowest (3.65%). Furthermore, the average body weight of this group was 192.23 mg, significantly higher than the other two groups. P <0.001 (Table 1). The above results show that the indoor rearing method for termite-preying creeping worms provided by the present invention adopts a nest-based group rearing strategy. By utilizing the kinship recognition behavior among individuals within the same nest, a low-aggression social structure is established in the group rearing, thereby significantly reducing cannibalistic losses. Combined with the comprehensive nutrition provided by the special artificial feed prepared in Example 1, a high survival rate and high-quality individual output of the population are achieved.
[0052] The present invention also obtained the predation ability of *Ceratophyllum demersum* on black-winged termites under different rearing conditions by fitting the Holling type II functional response model, as shown in Table 2.
[0053] Table 2. Predation ability of *Ceratophyllum demersum* on black-winged subterranean termites under different rearing conditions Note: In Table 2 N a For prey quantity, N It's the density of termites. a' It is the instantaneous attack rate of a predator on its prey. T h That is the processing time.
[0054] Table 2 shows that the predatory function parameters of *Termite-preying *Ceratophyllum demersum* were optimal in Example 2, confirming the synergistic effect of the indoor rearing method for *Termite-preying *Ceratophyllum demersum* provided by this invention. The adults obtained in Example 2 had the highest instantaneous attack rate (a′=0.972), and the treatment time ( T h=0.002 d) is the shortest, therefore its theoretical maximum daily predation (1 / T h =500 heads) and pest control effectiveness (a′ / T h =486.000) are significantly better than Comparative Example 1 (1 / T h =200 heads, a′ / T h =182.600) and Comparative Example 2 (1 / T h =250 heads, a′ / T h =205.500). Although Comparative Example 2 used the same feed as Example 2, its predation function parameters were still significantly lower than those of Example 2. This fully demonstrates that the special artificial feed provided by the present invention and the four-stage behavior management process have a significant synergistic effect in improving the predation performance of natural enemies, and the combination of the two is a necessary condition for achieving the continuous and efficient pest control ability of natural enemies. As can be seen from Tables 1 and 2: compared with Comparative Example 3, although there was no significant difference in reproductive behavior indicators such as egg hatching rate and egg-eating rate during the egg-guarding period, the average weight of adults bred in Example 2 (192.23 mg) was significantly higher than that in Comparative Example 3 (180.50 mg), and the instantaneous attack rate and maximum daily predation amount (500 vs 333) against black-winged subterranean termites were significantly better than those in Comparative Example 3. This indicates that the special artificial feed of the present invention plays an irreplaceable key role in improving the individual quality and predation efficiency of natural enemies.
[0055] The experimental data in Tables 1 and 2 show that this invention integrates three core technology modules—biomimetic soil environment-induced natural reproduction, management of littermate affinity behavior to reduce losses, and specific nutrient supply to maintain performance—to form a tightly linked breeding system. This system not only achieves breakthroughs in production indicators such as egg hatching rate and population survival rate, but more importantly, it significantly improves key functional indicators such as instantaneous attack rate and maximum daily predation rate. This systematically solves the three core problems of large-scale rearing of *Cyprinus erythrophagus*: difficulty in reproduction, high losses, and easy degradation of effectiveness, laying a solid foundation for its industrial production and application as a highly efficient and specialized natural enemy.
[0056] The above are merely preferred embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention.
Claims
1. A method for indoor rearing of termite-preying crickets, characterized in that, The process includes the following stages: independent nest building and breeding stage, mother-infant co-nursing stage, litter-group rearing stage, and homologous population maintenance stage, during which special artificial feed is provided.
2. The method for indoor rearing of termite-preying crickets according to claim 1, characterized in that, The special artificial feed comprises the following components by weight percentage: 25% black-winged termite powder, 20% mealworm powder, 35% carrot pulp, 0.1% potassium sorbate, 1% compound vitamin and mineral premix, and the remainder is water.
3. The method for indoor rearing of termite-preying crickets according to claim 1, characterized in that, The specific operation of the independent nesting and breeding stage is as follows: the mated male and female adult worms are placed in an independent breeding container with soil substrate at the bottom according to the pairing method, and raised in a quiet and dark environment. After the female worm digs a nest in the soil substrate and lays eggs, the male worm is removed.
4. The method for indoor rearing of termite-preying creeping crickets according to claim 3, characterized in that, The soil matrix has a thickness of 2-3 cm and a moisture content of 20-25%. During the independent nesting and breeding stage, the temperature was controlled at 25±1℃ and the relative humidity at 85±5%.
5. The method for indoor rearing of termite-preying crickets according to claim 1, characterized in that, The specific operation of the mother-infant co-breeding stage is as follows: maintain the same breeding conditions in the independent breeding box as in the independent nesting and breeding stage, wait for the eggs to hatch naturally in the nest, and let the female insect and the hatched nymph live together.
6. The method for indoor rearing of termite-preying creeping crickets according to claim 5, characterized in that, The period of cohabitation is 30 days.
7. The method for indoor rearing of termite-preying maggots according to claim 1, characterized in that, The specific operation of the same-nest group rearing stage is as follows: transfer the entire brood of nymphs hatched in the same nest during the mother-infant co-rearing stage to an independent rearing box, remove the female nymphs, maintain the same rearing conditions as the mother-infant co-rearing stage, and continue to rear the nymphs until they emerge as adults.
8. The method for indoor rearing of termite-preying creeping crickets according to claim 7, characterized in that, During the transfer process, the entire nymphal colony and part of the original nest soil from the mother-infant co-rearing stage were transferred to an independent rearing box. The stocking density during the littermate separation and rearing stage is 5-8 individuals / 100cm². 2 .
9. The method for indoor rearing of termite-preying creeping crickets according to claim 1, characterized in that, The specific operation of the homologous population maintenance stage is as follows: adults from the same nest are raised together as a homologous population unit in a final rearing container, and the rearing conditions are kept consistent with the mother-infant co-rearing stage.
10. The method for indoor rearing of termite-preying creeping crickets according to claim 1, characterized in that, The homologous population maintenance stage also includes placing the final rearing containers carrying different homologous population units into a lifetime rearing unit collection box. The lifetime rearing unit collection box is equipped with a unified ventilation channel and a centralized feed and water supply point.