A small line angle of the wood borer larva artificial feed and its preparation method and application

Abstract

The present application belongs to the technical field of insect feeding, and provides a kind of artificial feed for small line angle wood borer larvae, and its preparation method and application.The artificial feeding special feed includes the following raw materials by weight: white waxwood shavings 90-110 parts, soybean powder 25-35 parts, corn flour 10-20 parts, agar powder 3-7 parts, sucrose 2-4 parts, yeast powder 2-4 parts, cholesterol 0.3-0.7 parts, potassium sorbate 0.3-0.7 parts, methyl paraben 0.3-0.7 parts, ascorbic acid 0.3-0.7 parts, soybean oil 3-7 parts, and water 90-110 parts.Through three months of feeding test, it is proved that the growth rate and weight gain of group B larvae are significantly better than those of other groups, and the growth state of the experimental group with added oil and fat is obviously better, which provides an efficient technical solution for the large-scale artificial feeding of small line angle wood borer larvae, and provides a stable insect source for related biological research and prevention and control technology development.

Description

Technical Field

[0001] This invention relates to the field of insect rearing technology, and in particular to an artificial feed for the larvae of the small-lined wood-boring moth, its preparation method, and its application. Background Technology

[0002] The small-horned wood-boring moth is a significant trunk-boring pest, primarily damaging various garden and economic tree species such as ash, poplar, and willow. Its larvae bore into the trunk, causing weakened growth, broken branches, and even the death of the entire tree, resulting in severe losses to forestry production and urban greening. Research on the biological characteristics and control techniques of the small-horned wood-boring moth requires a stable supply of insects, and artificial rearing is a key method for obtaining standardized insect sources.

[0003] Currently, there are no systematic reports on specialized feeds and standardized rearing methods for the artificial rearing of *Small-lined Carpenter's Moth* larvae. Existing artificial feeds for carpenter's moths are mostly designed for specific species and do not fully consider the nutritional requirements of *Small-lined Carpenter's Moth*, resulting in unstable rearing effects, low larval survival rates, and slow growth and development. In particular, the lack of research on the role of nutrients in the growth of *Small-lined Carpenter's Moth* larvae limits the development of large-scale artificial rearing techniques. Therefore, developing a specialized feed and efficient rearing method suitable for the growth and development of *Small-lined Carpenter's Moth* larvae is of significant practical importance. Summary of the Invention

[0004] The purpose of this invention is to provide an artificial feed for the larvae of the small-lined carpenter moth, its preparation method, and its application. Addressing the current gaps and deficiencies in the artificial rearing technology for small-lined carpenter moth larvae, the primary objective of this invention is to develop a specialized artificial feed for these larvae with a balanced nutritional ratio and excellent rearing results, to meet the needs of large-scale rearing. Simultaneously, it provides a standardized and highly controllable artificial rearing method for small-lined carpenter moth larvae, ensuring a stable and reliable source of insects for related scientific research.

[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides a special feed for the artificial rearing of larvae of the small-lined wood-boring moth, comprising the following raw materials in parts by weight: 90-110 parts ash sawdust, 25-35 parts soybean flour, 10-20 parts corn flour, 3-7 parts agar powder, 2-4 parts sucrose, 2-4 parts yeast extract, 0.3-0.7 parts cholesterol, 0.3-0.7 parts potassium sorbate, 0.3-0.7 parts methylparaben, 0.3-0.7 parts ascorbic acid, 3-7 parts soybean oil, and 90-110 parts water.

[0006] Preferably, the ash wood chips are from 8-10 year old ash trees, and the moisture content of the ash wood chips is <10%.

[0007] The present invention also provides a method for preparing the artificially bred feed, comprising the following steps: (1) Mix agar powder, sucrose, yeast extract, cholesterol, potassium sorbate, methylparaben, ascorbic acid and water to obtain mixture 1; (2) Mix mixture 1 with soybean oil to obtain mixture 2; (3) Mix mixture 2 with ash wood chips, soybean flour and corn flour to obtain mixture 3, sterilize it, and you will get artificial feed.

[0008] Preferably, the mixing time in step (2) is 3 to 5 minutes.

[0009] Preferably, the mixing time in step (3) is 5-10 min, the sterilization temperature is 110-130℃, and the sterilization time is 25-35 min.

[0010] The present invention also provides a method for raising larvae of the small-horned wood-boring moth, comprising the following steps: The larvae of the small-lined wood-boring moth were fed with the aforementioned special artificial feed. The standard for adding the artificially raised special feed is 1~2g / head / time, and the frequency of changing the artificially raised special feed is 5~7 days / time.

[0011] Preferably, the rearing period is 2 to 4 months, the rearing temperature is 23 to 27°C, and the relative humidity is 60% to 70%.

[0012] The beneficial effects of this invention are as follows: This invention is the first to develop a special artificial feed for the larvae of the small-lined wood-boring moth. Through comparative experiments of four formulas, Group B was identified as the optimal formula. This formula is nutritionally balanced, retaining the ash wood chips that the larvae naturally feed on, while adding nutritional supplements such as soybean flour, corn flour, and yeast extract. In particular, the addition of soybean oil significantly promotes the growth and development of the larvae, solving the problem of the single nutritional content of existing artificial feeds for wood-boring moths.

[0013] A three-month feeding trial showed that the weight gain of larvae in group B was significantly higher than that in groups A (control group), C, and D. The larvae in groups B and C with added oil showed significantly better growth than those in groups A and D without added oil. This confirmed the important role of oily components in the growth of larvae of the small-lined wood-boring moth and provided a scientific basis for subsequent feed optimization.

[0014] The breeding method is standardized and highly controllable, clearly defining the cultivation temperature, humidity, light conditions, and feed replacement cycle. The operation process is simple and easy to implement, resulting in high larval survival rate and stable growth and development. This method enables large-scale artificial breeding of small-lined wood-boring moth larvae, providing a stable and reliable source of insects for related biological research, breeding of natural enemy insects, and development of control technologies. Attached Figure Description

[0015] Figure 1 Comparison images of larvae in each group. Detailed Implementation

[0016] This invention provides a special feed for the artificial rearing of larvae of the small-lined wood-boring moth, comprising the following raw materials in parts by weight: 90-110 parts ash sawdust, 25-35 parts soybean flour, 10-20 parts corn flour, 3-7 parts agar powder, 2-4 parts sucrose, 2-4 parts yeast extract, 0.3-0.7 parts cholesterol, 0.3-0.7 parts potassium sorbate, 0.3-0.7 parts methylparaben, 0.3-0.7 parts ascorbic acid, 3-7 parts soybean oil, and 90-110 parts water.

[0017] In this invention, the artificially raised feed is further preferably composed of the following raw materials in parts by weight: 100 parts ash wood chips, 30 parts soybean flour, 15 parts corn flour, 5 parts agar powder, 3 parts sucrose, 3 parts yeast extract, 0.5 parts cholesterol, 0.5 parts potassium sorbate, 0.5 parts methylparaben, 0.5 parts ascorbic acid, 5 parts soybean oil, and 100 parts water.

[0018] In this invention, the ash wood chips are preferably from 8-10 year old ash trees, more preferably from 9-year-old ash trees, and the moisture content of the ash wood chips is preferably <10%, more preferably 9%.

[0019] The present invention also provides a method for preparing the artificially bred feed, comprising the following steps: (1) Mix agar powder, sucrose, yeast extract, cholesterol, potassium sorbate, methylparaben, ascorbic acid and water to obtain mixture 1; (2) Mix mixture 1 with soybean oil to obtain mixture 2; (3) Mix mixture 2 with ash wood chips, soybean flour and corn flour to obtain mixture 3, sterilize it, and you will get artificial feed.

[0020] In this invention, the mixing time in step (2) is preferably 3 to 5 minutes, and more preferably 4 minutes.

[0021] In this invention, the mixing time in step (3) is preferably 5 to 10 minutes, more preferably 8 minutes, the sterilization temperature is preferably 110 to 130°C, more preferably 120°C, and the sterilization time is preferably 25 to 35 minutes, more preferably 30 minutes.

[0022] The present invention also provides a method for raising larvae of the small-horned wood-boring moth, comprising the following steps: The larvae of the small-lined wood-boring moth were fed with the aforementioned special artificial feed. The standard for adding the artificially raised special feed is 1~2g / head / time, and the frequency of changing the artificially raised special feed is 5~7 days / time.

[0023] In this invention, the addition standard of the artificially raised special feed is further preferably 1.5g / head / time, and the replacement frequency of the artificially raised special feed is further preferably 6 days / time.

[0024] In this invention, the breeding cycle is preferably 2 to 4 months, more preferably 3 months, the breeding temperature is preferably 23 to 27°C, more preferably 25°C, and the relative humidity of the breeding is preferably 60 to 70%, more preferably 65%.

[0025] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0026] Example 1

[0027] 1. Test insects

[0028] In early September 2025, 400 healthy larvae of the small-lined wood-boring moth were collected from the ash forest in Xiong'an New Area, Hebei Province. After being brought back to the laboratory, they were first placed in an artificial climate chamber at a temperature of 25°C and a relative humidity of 65% for 24 hours without being fed. After the larvae adapted to the indoor environment and emptied their intestinal contents, healthy and active larvae of the same age were selected for subsequent artificial rearing experiments.

[0029] 2. Feeding methods

[0030] 1) Preparation of artificial feed for larvae of the small-lined wood-boring moth

[0031] Nine-year-old ash tree segments were collected from ash forests in Xiong'an New Area, Hebei Province. After removing damaged bark, the branches were cut into 1cm pieces and ground into ash wood chips using a plant shredder. The chips were then dried until the moisture content was below 10% and sealed for later use. This embodiment includes four different artificial feed formulations: Group A (control group), Group B, Group C, and Group D. The specific formulations are shown in Table 1.

[0032] Table 1. Feed formulation for larvae of the small-lined wood-boring moth.

[0033] The specific preparation steps are as follows: 1) Weigh out the agar powder, sucrose, yeast extract, cholesterol, potassium sorbate, methylparaben, and ascorbic acid according to the specified amounts, put them into a 1000g beaker, add the corresponding volume of purified water (100g for group A, group B, and group D, and 120g for group C), and stir continuously with a glass rod until all ingredients are completely dissolved. 2) Adding oil (Groups B and C only): After the above ingredients are fully combined, add the amount of soybean oil specified in the formula, stir for 4 minutes to ensure that the oil is evenly dispersed and to avoid separation; 3) Mixing solid components: Add the prescribed amounts of ash wood chips, soybean flour, and corn flour, and continue stirring for 8 minutes to fully mix the solid and liquid components; 4) Dispensing: Pour the mixed material into 15×90mm glass petri dishes, and dispense 15g into each box; 5) Sterilize and store for later use: Place in a high-temperature sterilizer and sterilize at 120°C for 30 minutes. After sterilization, allow the feed to cool naturally to room temperature before storing for later use.

[0034] Example 2 Artificial Rearing Method

[0035] 1) Feed packaging: The four types of feed A, B, C and D prepared above are packaged into 15×90mm glass petri dishes, with 15g of feed in each dish.

[0036] 2) Larval Inoculation: After the feed has cooled to room temperature, using sterilized tweezers, place the temporarily reared *Caryota simonii* larvae one by one into the corresponding glass petri dishes, 100 larvae per group, ensuring that the larvae can burrow downwards along the petri dish wall to feed. After inoculation, label the glass petri dishes with the experimental group, inoculation date, and initial larval number.

[0037] 3) Rearing and Management: All inoculated glass petri dishes were placed in an artificial climate incubator for unified rearing. Rearing conditions were set as follows: temperature 25℃, relative humidity 65%, and darkness. During the rearing period, the feed was changed every 6 days. At each feed change, the larvae's weight was measured using an electronic balance with an accuracy of 0.001g. Simultaneously, the larvae's feeding and molting activities were observed and recorded in detail. This rearing experiment lasted for 3 months, from September 2025 to December 2025.

[0038] Example 3: Statistical Analysis of Feeding Results

[0039] This experiment involved feeding four groups of *Spodoptera litura* larvae for three months, regularly measuring and recording changes in larval weight, calculating the survival rate of larvae in each group, and analyzing the effects of different feed formulations on larval growth and development. The results are as follows: Figure 1 As shown in Table 2.

[0040] Table 2. Average weight growth trend and developmental indicators of *Small-lined Carpenter's Moth* larvae.

[0041] Analysis of the feeding results showed that among the four experimental groups, the larvae in group B had the best growth and development, with their average weight gain being significantly higher than that in groups A (control group), C, and D. In terms of growth and development differences, the larvae in group B, which had soybean oil added, had a significantly better weight gain rate than the other groups, indicating that adding oil to artificial feed can significantly promote the growth and development of the larvae of the small-lined wood-boring moth.

[0042] Furthermore, Group A, as the control group containing only ash sawdust and agar powder, showed a low larval survival rate and poor feeding activity. Some larvae stopped feeding and died in the later stages of rearing. Not only was the survival rate low, but the larvae also experienced weight loss due to nutrient depletion in the later stages, further confirming that host sawdust alone cannot meet the larval growth needs throughout the entire process. Group C did not add ash sawdust, and its main nutrient sources were soybean meal and corn meal. Although the larvae could feed, their growth rate was slower than that of Groups B and D, indicating that adding natural host sawdust can improve the palatability of the feed and is more in line with the feeding habits of the small-lined wood-boring moth larvae. The only difference between Group D and Group B was the absence of soybean oil. The larval growth and development indicators of Group D were significantly worse than those of Group B, further verifying the promoting effect of oil on the growth and development of the small-lined wood-boring moth larvae.

[0043] Further analysis of weight gain and survival rate indicators showed that the weight gain of group B larvae reached 0.133g, which was 33 times that of group A, 2.1 times that of group C, and 1.4 times that of group D. Moreover, the survival rate of group B larvae was as high as 90%, which was the highest among all groups. This indicates that group B larvae not only had the fastest growth rate, but also strong group vitality, high uniformity of growth and development, and no obvious individual differences, which meets the requirements of standardized insect sources for large-scale artificial breeding.

[0044] In comparison, Group A, due to its limited nutrition, only gained 0.004g of weight and experienced a subsequent decline in weight, resulting in a survival rate of only 10%, confirming that a single host substrate cannot meet the growth needs of larvae. Group C, lacking ash sawdust, suffered from insufficient palatability, resulting in a weight gain 53.4% ​​lower than Group B. Group D, without the addition of soybean oil, had a weight gain 31% lower than Group B, further validating the crucial promoting effects of unsaturated fatty acids provided by soybean oil and ash sawdust on larval growth.

[0045] In summary, the Group B feed, through its scientifically formulated blend of natural host components and diverse nutrients, achieves a triple advantage of high larval survival rate, high growth rate, and high growth stability, making it the optimal formula for large-scale artificial rearing of small-lined wood-boring moth larvae.

[0046] As can be seen from the above embodiments, the present invention provides an artificial feed for the larvae of the small-lined wood-boring moth, its preparation method, and its application. The artificial feed comprises the following raw materials in parts by weight: 90-110 parts ash sawdust, 25-35 parts soybean flour, 10-20 parts corn flour, 3-7 parts agar powder, 2-4 parts sucrose, 2-4 parts yeast extract, 0.3-0.7 parts cholesterol, 0.3-0.7 parts potassium sorbate, 0.3-0.7 parts methylparaben, 0.3-0.7 parts ascorbic acid, 3-7 parts soybean oil, and 90-110 parts water. Through a three-month rearing trial, the present invention has demonstrated that the growth rate and weight gain of larvae in group B were significantly better than those in other groups. The experimental group with added oil showed significantly better growth. This invention provides a stable and efficient technical solution for the large-scale artificial rearing of small-lined wood-boring moth larvae and provides a stable source of insects for related biological research and control technology development.

[0047] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A special feed for the artificial rearing of larvae of the small-lined wood-boring moth, characterized in that, The ingredients include the following parts by weight: 90-110 parts ash wood chips, 25-35 parts soybean flour, 10-20 parts corn flour, 3-7 parts agar powder, 2-4 parts sucrose, 2-4 parts yeast extract, 0.3-0.7 parts cholesterol, 0.3-0.7 parts potassium sorbate, 0.3-0.7 parts methylparaben, 0.3-0.7 parts ascorbic acid, 3-7 parts soybean oil, and 90-110 parts water.

2. The artificial breeding feed according to claim 1, characterized in that, The ash wood chips are from 8-10 year old ash trees, and the moisture content of the ash wood chips is <10%.

3. The method for preparing the artificially raised feed according to claim 1, characterized in that, Includes the following steps: (1) Mix agar powder, sucrose, yeast extract, cholesterol, potassium sorbate, methylparaben, ascorbic acid and water to obtain mixture 1; (2) Mix mixture 1 with soybean oil to obtain mixture 2; (3) Mix mixture 2 with ash wood chips, soybean flour and corn flour to obtain mixture 3, sterilize it, and you will get artificial feed.

4. The preparation method according to claim 3, characterized in that, The mixing time in step (2) is 3 to 5 minutes.

5. The preparation method according to claim 4, characterized in that, The mixing time in step (3) is 5-10 min, the sterilization temperature is 110-130℃, and the sterilization time is 25-35 min.

6. A method for raising larvae of the small-horned wood-boring moth, characterized in that, Includes the following steps: The artificial feeding feed described in claim 1 was used to feed the larvae of the small-lined wood-boring moth; The standard for adding the artificially raised special feed is 1~2g / head / time, and the frequency of changing the artificially raised special feed is 5~7 days / time.

7. The method according to claim 6, characterized in that, The rearing period is 2 to 4 months, the rearing temperature is 23 to 27°C, and the relative humidity is 60% to 70%.

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