Preparation method of kapok flower extract, product and application thereof

Abstract

This invention relates to a method for preparing a kapok flower extract. The method includes the following steps: mixing kapok flower raw materials with an ionic reagent and extracting under a high-voltage pulsed electric field; centrifuging, filtering, and collecting the filtrate to obtain the kapok flower extract; the ionic reagent is obtained by reacting glycerol and betaine. This invention utilizes a natural ionic reagent combined with a high-voltage pulsed extraction method. First, a high-voltage pulsed electric field is used to induce biomembrane permeation, causing the relevant active substances in the kapok flower to dissolve. Simultaneously, the ionic reagent can effectively improve the extraction efficiency of relevant active ingredients in the kapok flower. Compared with conventional water extracts, the extract prepared by the process provided by this invention has a significantly increased content of active ingredients and also possesses advantages such as antibacterial, antioxidant, and long shelf life. When applied to skincare products, it can effectively resist oxidation, improve skin radiance, and delay skin aging.

Description

Technical Field

[0001] This invention belongs to the field of plant extraction technology, specifically relating to a method for preparing kapok flower extract, its products, and applications. Background Technology

[0002] Kapok (scientific name: Kapok) Bombax ceiba *Ceiba speciosa*, also known as the climbing tree or spotted tree, is a deciduous tree belonging to the genus *Ceiba* in the Malvaceae family. Its bark is grayish-white, and young trees typically have conical, coarse thorns on their trunks. The branches are spreading. The leaves are palmately compound with 5-7 leaflets, oblong to oblong-lanceolate in shape. Flowers are solitary in the leaf axils at the branch tips, usually red, sometimes orange-red, about 10 cm in diameter. The calyx is cup-shaped, 2-3 cm long, glabrous outside, densely covered with pale yellow short silky hairs inside, with 3-5 semi-circular calyx teeth. The petals are fleshy, obovate-oblong. The capsule is oblong and obtuse. It is distributed in India, Sri Lanka, the Indochina Peninsula, Malaysia, Indonesia to the Philippines, northern Australia, and China. In China, it is distributed in subtropical provinces. *Ceiba speciosa* grows in dry-hot valleys and savannas below 1400 (-1700) meters in altitude, and can also grow in monsoon forests in valleys.

[0003] Kapok flower extract contains a large number of natural active ingredients with excellent efficacy. According to the Chinese Pharmacopoeia, kapok flower has the functions of "clearing heat and dampness, and detoxifying." Therefore, kapok flower is not only an ornamental plant but also a medicinal and edible material, which can be used to make herbal teas, flower teas, soups, and throat lozenges. In addition, kapok flower extract contains antioxidants, allowing it to be added to cosmetics and skincare products for anti-aging and whitening effects. Therefore, developing a new, safe, environmentally friendly, and efficient method for preparing kapok flower extract is of significant practical importance. Summary of the Invention

[0004] In view of the shortcomings of the prior art, the purpose of this invention is to provide a method for preparing kapok flower extract, as well as its products and applications.

[0005] To achieve this objective, the present invention adopts the following technical solution: In a first aspect, the present invention provides a method for preparing a kapok flower extract, the method comprising the following steps: The kapok flower raw material was mixed with an ionic reagent and extracted under a high-voltage pulsed electric field. After centrifugation and filtration, the filtrate was collected to obtain the kapok flower extract. The ionic reagent is obtained by reacting glycerol and betaine.

[0006] This invention utilizes a combination of natural ionic reagents and high-pressure pulse extraction. First, a high-pressure pulsed electric field induces the permeation of the biological membrane, allowing the relevant active substances in kapok flowers to dissolve. Simultaneously, the ionic reagents effectively improve the extraction efficiency of the relevant functional components from kapok flowers. Compared to conventional water extracts, the extract prepared by the process provided by this invention significantly increases the content of active ingredients and also possesses advantages such as antibacterial, antioxidant properties, and a long shelf life. Furthermore, it is faster and more efficient than either ionic reagent extraction or high-pressure pulsed electric field water extraction. Moreover, it has been further discovered that when glycerol and betaine are used together as extraction solvents for kapok flowers, they synergistically enhance the dissolution of relevant functional components, resulting in a superior extract product.

[0007] Preferably, the molar ratio of glycerol to betaine is 1:1 to 5:1, for example, 1:1, 2:1, 3:1, 4:1, 5:1, etc.

[0008] Preferably, the kapok flower raw material is kapok flower powder after being crushed and sieved.

[0009] Preferably, the mesh size of the sieve used in the sieving process is 20-200 mesh, for example, it can be 20 mesh, 25 mesh, 30 mesh, 35 mesh, 40 mesh, 45 mesh, 50 mesh, 80 mesh, 100 mesh, 120 mesh, 150 mesh, 180 mesh, 200 mesh, etc. Other specific values ​​within this range can be selected, and will not be described in detail here; preferably, it is 30-80 mesh.

[0010] Preferably, the ionic reagent is mixed with water before being mixed with the kapok flower raw material.

[0011] Preferably, the mass ratio of the ionic reagent to water is 1:1 to 1:4, for example, it can be 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, etc.

[0012] In this invention, the ionic reagent is mixed with water in a specific ratio, which can control the state of the extraction system during extraction while ensuring the extraction effect. This ensures that the active ingredients in the extracted ionic reagent can be effectively dissolved after extraction, thereby further enhancing the skin-improving effects of the final extract product.

[0013] Preferably, the solid-liquid ratio of the kapok flowers and water is 1:(20-40) g / mL, for example, it can be 1:20, 1:25, 1:28, 1:30, 1:32, 1:35, 1:38, 1:40, etc. Other specific values ​​within this range can be selected, and will not be elaborated here.

[0014] Preferably, the field strength extracted by the high-voltage pulse electric field is 30-50 kV / m, for example, it can be 30 kV / m, 35 kV / m, 40 kV / m, 45 kV / m, 50 kV / m, etc. Other specific point values ​​within this range can be selected, and will not be elaborated here.

[0015] In this invention, a high-voltage pulsed electric field extraction process with specific field strength parameters is used to generate transmembrane potential in cell membranes, increasing cell membrane permeability and making the active ingredients in kapok flowers easier to dissolve. It can also effectively reduce the decomposition of the dissolved active ingredients caused by the heat generated during extraction, thereby effectively improving the skin-improving effects in the final product.

[0016] Preferably, the number of electric shocks extracted by the high-voltage pulse electric field is 80-120 times, for example, it can be 80 times, 85 times, 90 times, 95 times, 100 times, 105 times, 110 times, 120 times, etc. Other specific values ​​within this range can be selected, which will not be elaborated here.

[0017] When high-voltage pulsed electric field extraction is used with a specific number of electric shocks, an electroporation effect can be generated, which promotes the release of cell wall contents and can effectively reduce the decomposition of related active ingredients caused by the heat generated during extraction, thereby effectively enhancing the skin-improving effects in the final product.

[0018] Preferably, after filtration, filter residue is collected, and the filter residue is mixed with a compound enzyme solution. The enzymatic hydrolysate is filtered to obtain a second filtrate, which is then combined with the filtrate obtained after high-voltage pulsed electric field extraction filtration to obtain the kapok flower extract. The enzymes in the complex enzyme solution include a combination of pectinase, cellulase and fig protease.

[0019] In this invention, the innovative two-step extraction process yields superior dissolution of the relevant active ingredients in kapok flowers. Specifically, the pretreated product is first extracted using a high-voltage pulsed electric field combined with ionic reagents, and the filtrate is collected. Simultaneously, the precipitate is subjected to enzymatic hydrolysis, and the hydrolysate filtrate is collected and combined. Compared to single high-voltage pulsed electric field extraction or single enzymatic hydrolysis, this method significantly increases the yield and flavonoid content of the extract, while also further enhancing the extract's moisturizing and antioxidant effects.

[0020] Preferably, the mass ratio of pectinase, cellulase and fig protease is (1-3):(1-3):(0.05-0.2); The "1-3" can be, for example, 1, 1.5, 2, 2.5, 3, etc. The "0.05-2" can be, for example, 0.05, 0.1, 0.2, 0.5, 0.8, 1, 1.2, 1.5, 1.8, 2, etc.; When the ratio of the three bio-enzymes in the compound enzyme used is within the above-mentioned specific range, the extraction effect of the effective components in kapok flowers can be further improved, resulting in better results.

[0021] Preferably, the enzymatic hydrolysis temperature is 40-60℃ (e.g., 40℃, 42℃, 45℃, 48℃, 50℃, 52℃, 55℃, 58℃, 60℃, etc.), and the time is 0.5-2 h (e.g., 0.5 h, 0.8 h, 1 h, 1.2 h, 1.5 h, 1.8 h, 2 h, etc.).

[0022] Preferably, the solid-liquid ratio of the filter residue to the composite enzyme solution is 1:(15-50) g / mL, for example, 1:15, 1:20, 1:30, 1:40, 1:50, etc.

[0023] Preferably, the amount of the compound enzyme added is 0.1‰-0.5‰ of the mass of the filter residue, for example, it can be 0.1‰, 0.2‰, 0.25‰, 0.3‰, 0.35‰, 0.4‰, 0.5‰, etc.

[0024] In a second aspect, the present invention provides a kapok flower extract, which is prepared by the preparation method described in the first aspect.

[0025] Thirdly, the present invention provides the application of the kapok flower extract according to the second aspect in cosmetics.

[0026] Compared with the prior art, the present invention has the following beneficial effects: This invention utilizes a combination of natural ionic reagents and high-pressure pulse extraction. First, a high-pressure pulsed electric field induces the permeation of the biological membrane, allowing the relevant active substances in kapok flowers to dissolve. Simultaneously, the ionic reagents effectively improve the extraction efficiency of the relevant functional components from kapok flowers. Compared to conventional water extracts, the extract prepared by the process provided by this invention significantly increases the content of active ingredients and also possesses advantages such as antibacterial, antioxidant properties, and a long shelf life. Furthermore, it is faster and more efficient than either ionic reagent extraction or high-pressure pulsed electric field water extraction. Moreover, it has been further discovered that when glycerol and betaine are used together as extraction solvents for kapok flowers, they synergistically enhance the dissolution of relevant functional components, resulting in a superior extract product. Detailed Implementation

[0027] The technical solution of the present invention will be further illustrated below through specific embodiments. Those skilled in the art should understand that the embodiments described are merely illustrative of the present invention and should not be construed as limiting the invention in any way.

[0028] The pectinase, cellulase and fig protease mentioned below are products from Shanghai Yuanye Biotechnology, with model numbers S10007, S10041 and V30095 respectively.

[0029] Example 1 This embodiment provides a kapok flower extract, the preparation method of which is as follows: Kapok flower raw materials were washed, dried, pulverized, and passed through a 40-mesh sieve. The pulverized kapok flowers were mixed with a pre-mixed ionic reagent (glycerol and betaine in a molar ratio of 2.5:1) with water. The mass ratio of the ionic reagent to water was 2:3, and the solid-liquid ratio of water to kapok flowers was 1:24 g / mL. High-voltage pulsed electric field extraction was performed under the following conditions: 35 kV / m, 110 electric shocks, stirring at 80 rpm for 30 min, centrifuging at 10000 rpm for 5 min, and then filtering the filtrate through a 0.22 μm filter membrane. The filtrate was collected and freeze-dried to obtain the kapok flower extract.

[0030] Example 2 This embodiment provides a kapok flower extract, the preparation method of which is as follows: Kapok flower raw materials were washed, dried, pulverized, and passed through a 60-mesh sieve. The pulverized kapok flowers were mixed with a pre-mixed ionic reagent (glycerol and betaine in a molar ratio of 1.5:1) with water. The mass ratio of the ionic reagent to water was 1:3, and the solid-liquid ratio of water to kapok flowers was 1:35 g / mL. High-voltage pulsed electric field extraction was performed under the following conditions: 30 kV / m, 120 electric shocks, stirring at 80 rpm for 30 min, centrifuging at 10,000 rpm for 5 min, and then filtering the filtrate through a 0.22 μm filter membrane. The filtrate was collected and freeze-dried to obtain the kapok flower extract.

[0031] Example 3 This embodiment provides a kapok flower extract, the preparation method of which is as follows: Kapok flower raw materials were washed, dried, pulverized, and passed through a 50-mesh sieve. The pulverized kapok flowers were mixed with a pre-mixed ionic reagent (glycerol and betaine in a molar ratio of 4:1) with water. The mass ratio of the ionic reagent to water was 1:1, and the solid-liquid ratio of water to kapok flowers was 1:35 g / mL. High-voltage pulsed electric field extraction was performed under the following conditions: 50 kV / m, 80 electric shocks, stirring at 80 rpm for 30 min, centrifuging at 10,000 rpm for 5 min, and then filtering the filtrate through a 0.22 μm filter membrane. The filtrate was collected and freeze-dried to obtain the kapok flower extract.

[0032] Example 4 This embodiment provides a kapok flower extract, which uses the kapok flower powder from Example 1. The only difference between this and Example 1 is that the mass ratio of the ionic reagent to water is 3:1. All other process steps and related parameters are the same as in Example 1.

[0033] Example 5 This embodiment provides a kapok flower extract, which uses the kapok flower powder from Example 1. The only difference between this and Example 1 is that the mass ratio of the ionic reagent to water is 1:6. All other process steps and related parameters are the same as in Example 1.

[0034] Example 6 This embodiment provides a kapok flower extract, which uses the kapok flower powder from Example 1. The only difference between this and Example 1 is that the solid-liquid ratio of the kapok flower and water is 1:10 g / mL. All other process steps and related parameters are the same as in Example 1.

[0035] Example 7 This embodiment provides a kapok flower extract, which uses the kapok flower powder from Example 1. The only difference between this and Example 1 is that the solid-liquid ratio of the kapok flower and water is 1:50 g / mL. All other process steps and related parameters are the same as in Example 1.

[0036] Example 8 This embodiment provides a kapok flower extract, which uses the kapok flower powder from Example 1. The only difference between this and Example 1 is that the field strength of the high-voltage pulse electric field extraction is 20 kV / m. All other process steps and related parameters are the same as in Example 1.

[0037] Example 9 This embodiment provides a kapok flower extract, which uses the kapok flower powder from Example 1. The only difference between this and Example 1 is that the field strength of the high-voltage pulse electric field extraction is 75 kV / m. All other process steps and related parameters are the same as in Example 1.

[0038] Example 10 This embodiment provides a kapok flower extract, which uses the kapok flower powder from Example 1. The only difference between this embodiment and Example 1 is that the number of electric shocks during high-voltage pulse electric field extraction is 60. All other process steps and related parameters are the same as in Example 1.

[0039] Example 11 This embodiment provides a kapok flower extract, which uses the kapok flower powder from Example 1. The only difference between this and Example 1 is that the number of electric shocks during high-voltage pulse electric field extraction is 160. All other process steps and related parameters are the same as in Example 1.

[0040] Example 12 This embodiment provides a kapok flower extract. The filter residue from the membrane filtration in Example 1 is mixed with pure water at a solid-liquid ratio of 1:30 g / mL, and then enzymatically hydrolyzed with a complex enzyme (composed of cellulase, pectinase, and fig protease in a mass ratio of 2:1:0.1) at 55°C for 2 h. The amount of the complex enzyme added is 0.3‰ of the filter residue. After the reaction is complete, an enzymatic hydrolysate is obtained. The hydrolysate is filtered to obtain a second filtrate. The second filtrate is combined with the filtrate obtained after membrane filtration in Example 1, and then freeze-dried to obtain the kapok flower extract.

[0041] Comparative Example 1 This comparative example provides a kapok flower extract, which is obtained by hot water extraction using the kapok flower powder from Example 1 at a temperature of 80°C for 60 min. The extract is then collected by stirring, centrifugation, filtration, and freeze-drying.

[0042] Comparative Example 2 This comparative example provides a kapok flower extract, which is obtained by extracting the kapok flower powder from Example 1 with an ionic reagent (glycerol and betaine in a molar ratio of 2.5:1) at an extraction temperature of 72°C for 60 min. The extract is then collected by stirring, centrifugation, filtration, and freeze-drying.

[0043] Comparative Example 3 This comparative example provides a kapok flower extract, which uses the kapok flower powder from Example 1, replacing the ionic reagent in Example 1 with an equal amount of water, and the remaining process steps and related parameters are the same as in Example 1.

[0044] Comparative Example 4 This comparative example provides a kapok flower extract, which is prepared by mixing kapok flower powder from Example 1 with pure water at a solid-liquid ratio of 1:30 g / mL, and then hydrolyzing it with a complex enzyme (composed of cellulase, pectinase, and fig protease in a mass ratio of 2:1:0.1) at 55°C for 2 h. The amount of the complex enzyme added is 0.3‰ of the filter residue. After the reaction is completed, the enzymatic hydrolysate is obtained, filtered, and the filtrate is freeze-dried to obtain the kapok flower extract.

[0045] Test Example 1 Antioxidant effect test (1) DPPH-free radical scavenging A 96-well plate was used, with three replicates per group, and the total volume was 200 μL. For the sample group: 100 μg of the extracts prepared in Examples 1-12 and Comparative Examples 1-4 were mixed with 100 μL of pure water, and 100 μL of 0.1 mM DPPH solution was added to the reaction system. For the control group: 100 μL of pure water was mixed with 100 μL of 0.1 mM DPPH solution. After the reaction system was constructed, it was shaken in the dark for 10 min, and the absorbance at 517 nm was measured using a microplate reader.

[0046] Method for calculating clearance rate: A0 represents the absorbance of the control group, and Ax represents the absorbance of the sample group.

[0047] (2) ABTS-free radical scavenging Preparation of ABTS working solution: Mix ABTS with potassium persulfate solution to prepare 7mM ABTS stock solution, store in the dark for 12h, shake well and mix with 10mM PBS (pH=7.4) so ​​that the absorbance of the solution at 734nm is 0.8, thus obtaining ABTS working solution.

[0048] A 96-well plate was used, with three replicates per group and a total volume of 200 μL. For the sample group, 100 μg of the extracts prepared in Examples 1-12 and Comparative Examples 1-4 were mixed with 10 μL of purified water, and 190 μL of ABTS working solution was added to the reaction system. For the control group, 10 μL of purified water was mixed with 190 μL of ABTS working solution. After the reaction system was constructed, it was thoroughly shaken and incubated for 6 min. The absorbance at 734 nm was measured using a microplate reader.

[0049] Method for calculating clearance rate: A0 represents the absorbance of the control group, and Ax represents the absorbance of the sample group.

[0050] The test results are shown in the table below.

[0051] Table 1 The data in the table shows that... (1) As can be seen from Examples 1 to 3, the cotton flower extract prepared by the present invention has excellent DPPH- and ABTS- free radical scavenging effects and exhibits significant antioxidant effects.

[0052] (2) By comparing Example 1 with Examples 4-5, it can be seen that when the mass ratio of ionic reagent to water is within a specific range, the extraction of active ingredients of kapok flowers can be increased, and the state of the extraction liquid system can be controlled as much as possible. This promotes the efficient dissolution of active ingredients from kapok flower raw materials into the extract, resulting in better antioxidant effects of the kapok flower extract prepared from it.

[0053] (3) By comparing Example 1 with Examples 6-7, it can be seen that when the amount of ionic reagent added is within a specific range, the contact area between the extraction solvent and the reactants is effectively increased, which can make the active ingredients in the kapok flower release quickly and efficiently, and a kapok flower extract with higher antioxidant effect is prepared.

[0054] (4) By comparing Example 1 with Examples 8-11, it can be seen that when the parameters of electric field strength and number of electric shocks of the high voltage pulse electric field used during extraction are selected within a specific range, the extraction effect on kapok flowers is better, and a kapok flower extract with higher antioxidant effect is prepared.

[0055] (5) By comparing Example 12 with Example 1 and Comparative Example 4, it can be seen that when the sediment after high voltage pulse electric field extraction is further subjected to biological enzymatic extraction, the two-step extraction operation can further increase the content of active ingredients in the extract compared with single high voltage pulse electric field extraction or single enzymatic treatment, and prepare a better kapok flower extract.

[0056] (6) By comparing Example 1 with Comparative Examples 1-3, it can be seen that the extract product prepared by the natural ionic reagent fusion high-pressure pulse extraction method has significantly improved effect compared with the extract products prepared by conventional hot water extraction, ionic reagent extraction alone, or high-pressure pulse electric field extraction alone.

[0057] Test Example 2 Anti-inflammatory effect test Test method: RAW264.7 mouse macrophages in logarithmic growth phase with good morphology were seeded in DMEM culture medium containing 10% FBS fetal bovine serum and incubated for 24 h, at a concentration of 2×10⁻⁶ cells / mL. 5 Cells were seeded at a density of cells / well in 24-well plates and incubated at 37°C with 5% CO2 for 2 h. Cells were then stimulated with 1 μg / mL lipopolysaccharide for 24 h to establish a cell inflammation model.

[0058] Add 0.8% of the kapok flower extract prepared in Examples 1-12 and Comparative Examples 1-4 to each group of cells. After incubation at 37°C for 24 h, collect the cell supernatant and perform detection according to the ELISA kit instructions. Detect the contents of TNF-α and IL-6 in the collected cell supernatant. Analyze the experimental results using data processing software. The results are shown in the table below.

[0059] Table 2 TNF-α and IL-6 are important pro-inflammatory mediators that mediate various inflammatory responses. Reducing their levels can inhibit inflammatory responses, thereby achieving an anti-inflammatory effect. The data in the table show that the kapok flower extract prepared by the method provided in this invention has excellent inflammatory factor regulation effects and exhibits significant anti-inflammatory efficacy. Specifically, the antioxidant effect of Example 1 is superior to that of Examples 4-11 and Comparative Examples 1-5, indicating that the specific preparation conditions of the kapok flower extract used in this invention have varying degrees of influence on the antioxidant efficacy of the final extract. Furthermore, when Example 12 further employs a bio-enzymatic hydrolysis combined with a high-voltage pulsed electric field extraction process, the anti-inflammatory effect of the prepared kapok flower extract is further enhanced compared to Example 1.

[0060] Test Example 3 Determination of total flavonoids in Kapok flower extract by ultraviolet spectrophotometry (1) Take 2.4 mL of rutin solutions with different concentration gradients (0-0.2 mg / mL), add 0.4 mL of 5% NaNO2 solution, shake to mix, and let stand for 6 min; then add 0.4 mL of 10% Al(NO3)3 solution, shake to mix, and let stand for 6 min; then add 4 mL of 4% NaOH solution, and then add pure water to make up to 10 mL, shake to mix, and let stand for 15 min. Zero the instrument with the corresponding solvent blank, and measure the absorbance value at 510 nm. Plot a standard curve with rutin concentration as the abscissa and 510 nm absorbance as the ordinate. The standard curve is y=0.0078x-0.0026.

[0061] (2) Take 2.4 mg of the extract samples prepared in Examples 1 and 12 respectively, mix them with 2.4 mL of pure water, then add 0.4 mL of 5% NaNO2 solution, shake to mix, and let stand for 6 min; then add 0.4 mL of 10% Al(NO3)3 solution, shake to mix, and let stand for 6 min; then add 4 mL of 4% NaOH solution, and then add pure water to make up to 10 mL, shake to mix, and let stand for 15 min. Zero the sample with the corresponding solvent blank, and measure the absorbance value at 510 nm. Perform three parallel samplings for each sample, and calculate the total flavonoid content in the extract sample. The formula is as follows: Total flavonoid content (mg / mL) = (Cx×10) / V×K×0.001, where 10 is the test volume in mL, and 0.001 is the unit conversion. Cx is the concentration calculated by substituting the test absorbance value into the standard curve, in μg / mL. V is the volume of the sample taken for testing, in mL. K is the sample dilution factor. The results are shown in the table below.

[0062] Table 3 As can be seen from the data in the table, the kapok flower extract prepared by this invention has a high content of flavonoid active substances and has high antioxidant activity, which can be used as a cosmetic raw material to greatly improve its antioxidant effect.

[0063] Test Example 4 Product safety testing Subjects were selected according to the inclusion criteria, with a male-to-female ratio of 1:1, and randomly divided into 12 groups (2 males and 2 females in each group) to receive the trial. An appropriate amount of the extract sample prepared in Examples 1-12 (the extract was mixed with pure water to adjust the extract concentration to 3% before testing) was placed in the drug compartment of the test strip. The test strip containing the sample was then applied to the flexor surface of the subject's forearm with medical tape for 24 hours. Thirty minutes after removing the test strip, and after the indentation disappeared, the skin reaction was observed. For those with negative results, the skin reaction was observed again at 24 hours and 48 hours after the trial, and the results were recorded. The grading criteria for the degree of reaction are shown in the table below.

[0064] Table 4 The results showed that the products prepared in Examples 1-12 all exhibited negative reactions at 24 h and 48 h after the test, with no irritation or erythema. Therefore, the kapok flower extract product prepared by this invention is non-irritating to the skin and shows no positive reaction, proving that the product is highly safe, has low irritation, and is relatively mild.

[0065] The applicant declares that the technical solution of this invention is illustrated by the above embodiments, but this invention is not limited to the above embodiments, that is, it does not mean that this invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials for the products of this invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of this invention.

[0066] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0067] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A method for preparing a kapok flower extract, characterized in that, The preparation method includes the following steps: The kapok flower raw material was mixed with an ionic reagent and extracted under a high-voltage pulsed electric field. After centrifugation and filtration, the filtrate was collected to obtain the kapok flower extract. The ionic reagent is obtained by reacting glycerol and betaine.

2. The method for preparing kapok flower extract according to claim 1, characterized in that, The raw material of kapok flowers is kapok flower powder after being crushed and sieved. Preferably, the sieve used in the sieving process has a mesh size of 20-200.

3. The method for preparing kapok flower extract according to claim 1, characterized in that, The ionic reagent is mixed with water before being mixed with the kapok flower raw material; Preferably, the mass ratio of the ionic reagent to water is 1:1 to 1:

4.

4. The method for preparing the kapok flower extract according to claim 3, characterized in that, The solid-liquid ratio of the kapok flowers and water is 1:(20-40) g / mL.

5. The method for preparing kapok flower extract according to claim 1, characterized in that, The field strength extracted from the high-voltage pulsed electric field is 30-50 kV / m; Preferably, the number of electric shocks extracted by the high-voltage pulse electric field is 80-120.

6. The method for preparing kapok flower extract according to claim 1, characterized in that, After filtration, filter residue is collected, and the filter residue is mixed with a compound enzyme solution. The enzyme hydrolysate is filtered to obtain a second filtrate, which is then combined with the filtrate obtained after high-voltage pulsed electric field extraction and filtration to obtain the kapok flower extract. The enzymes in the complex enzyme solution include a combination of pectinase, cellulase and fig protease.

7. The method for preparing kapok flower extract according to claim 6, characterized in that, The mass ratio of pectinase, cellulase and fig protease is (1-3):(1-3):(0.05-0.2); Preferably, the enzymatic hydrolysis is performed at a temperature of 40-60°C for 0.5-2 hours.

8. The method for preparing kapok flower extract according to claim 6, characterized in that, The solid-liquid ratio of the filter residue to the compound enzyme solution is 1:(15-50) g / mL; Preferably, the amount of the compound enzyme added is 0.1‰-0.5‰ of the mass of the filter residue.

9. A kapok flower extract, characterized in that, The kapok flower extract is prepared by the preparation method according to any one of claims 1-8.

10. The use of the kapok flower extract according to claim 9 in cosmetics.

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