Microfluidic detection chip and detection equipment for multi-path quantitative dispensing

Abstract

The application discloses a microfluidic detection chip and a detection equipment for multi-path quantitative proportioning. The detection equipment comprises a microfluidic detection chip for multi-path quantitative proportioning. The microfluidic detection chip for multi-path quantitative proportioning comprises a main body, wherein at least a reagent distribution area, a sample inlet area, a first reagent quantitative area, a second reagent quantitative area, a sample quantitative area, a mixing cavity, a buffer cavity, a second quantitative cavity, a first detection area and a second detection area are arranged on the main body. The reagent distribution area is communicated with the first reagent quantitative area, the second reagent quantitative area and the mixing cavity respectively. The sample inlet area is communicated with the sample quantitative area. The sample quantitative area is communicated with the mixing cavity. The buffer cavity is communicated with the mixing cavity. The second quantitative cavity is communicated with the buffer cavity. The first reagent quantitative area is further communicated with the second quantitative cavity and the first detection area. The second detection area is communicated with the buffer cavity. The microfluidic detection chip and the detection equipment for multi-path quantitative proportioning solve the problem that the microfluidic detection chip is difficult to support the proportioning of samples and reagents of different detection items in the prior art.

Description

Technical Field

[0001] This application relates to the field of microfluidic detection technology, and more specifically, to a microfluidic detection chip and detection device for multi-channel quantitative mixing. Background Technology

[0002] Microfluidic detection chips for multi-channel quantitative mixing utilize centrifugal force to achieve the distribution, mixing, reaction, and detection of blood samples. They have the advantages of simple operation, no need for external pumps or valves, and batch processing capability, making them particularly suitable for the need for automated sample processing workflows.

[0003] In related technologies, by setting a quantitative region on a microfluidic detection chip for multi-channel quantitative preparation, the chip can achieve the corresponding ratio of sample to reagent after centrifugation. However, when the microfluidic detection chip for multi-channel quantitative preparation is used to perform at least two different tests on blood, it is difficult to support the ratio of samples to reagents for different tests. Summary of the Invention

[0004] The main objective of this application is to provide a microfluidic detection chip and detection device for multi-channel quantitative mixing, so as to at least solve the problem in the related art that microfluidic detection chips cannot support the mixing ratio of samples and reagents for different detection items.

[0005] According to one aspect of this application, a microfluidic detection chip for multi-channel quantitative mixing is provided, comprising: The main body includes at least a reagent dispensing area, a sample injection area, a first reagent quantification area, a second reagent quantification area, a sample quantification area, a mixing chamber, a buffer chamber, a second quantification chamber, a first detection area, and a second detection area. The reagent dispensing area is connected to the first reagent quantification area, the second reagent quantification area, and the mixing chamber, respectively. The sample injection area is connected to the sample quantification area. The sample quantification area is connected to the mixing chamber. The buffer chamber is connected to the mixing chamber. The second quantification chamber is connected to the buffer chamber. The first reagent quantification area is also connected to the second quantification chamber and the first detection area. The second detection area is connected to the buffer chamber. Wherein, along the direction from the center of the main body to the outer edge of the main body, the distances from the reagent dispensing area, the second reagent quantification area, and the first detection area to the center of the main body increase sequentially, the distances from the reagent dispensing area, the first reagent quantification area, and the first detection area to the center of the main body increase sequentially, the distances from the sample injection area, the sample quantification area, the mixing chamber, the buffer chamber, the second quantification chamber, the first reagent quantification area, and the second detection area to the center of the main body increase sequentially, and the mixing chamber is used to prepare a first liquid in a first predetermined ratio, and the first reagent quantification area, the second reagent quantification area, the second quantification chamber, and the first detection area work together to prepare a second liquid in a second predetermined ratio.

[0006] Furthermore, the first reagent quantification region includes: A first quantitative chamber is connected to the reagent dispensing area via a siphon pipe; A first processing chamber is connected to a first quantitative chamber, and the first processing chamber is connected to the first detection area and the second quantitative chamber respectively through a siphon pipe. A first reaction reagent is stored in the first processing chamber. Wherein, along the direction from the center of the main body to the outer edge of the main body, the distance from the reagent dispensing area, the first quantitative cavity, the second quantitative cavity and the first processing cavity to the center of the main body increases sequentially, and the first processing cavity is used to mix the first reaction reagent and the reagent flowing into the first processing cavity from the first quantitative cavity and the second quantitative cavity respectively with the first liquid to form a third liquid.

[0007] Furthermore, the second reagent quantification area also includes a storage chamber, which is connected to the first quantification chamber and the first processing chamber via siphon pipes; In this context, along the direction from the center of the main body to its outer edge, the distances from the first metering cavity, the storage cavity, and the first processing cavity to the center of the main body increase sequentially.

[0008] Furthermore, the second reagent quantification area includes a third quantification chamber, which is connected to the reagent dispensing area and the first detection area via siphon pipes; Specifically, along the direction from the center of the main body to its outer edge, the distances from the reagent dispensing area, the third quantitative cavity, and the first detection area to the center of the main body increase sequentially.

[0009] Furthermore, the second reagent quantification region also includes: A transfer chamber, wherein the transfer chamber is connected between the third quantitative chamber and the reagent dispensing area, and the transfer chamber is connected to the first reagent quantitative area; The first waste liquid chamber is connected to the third quantitative chamber, and the distances from the reagent dispensing area, the transfer chamber, the third quantitative chamber, and the first waste liquid chamber to the center of the main body increase sequentially along the direction from the center of the main body to the outer edge of the main body.

[0010] Furthermore, the microfluidic detection chip for multi-channel quantitative mixing also includes a waste liquid chamber, which is connected to the second quantitative chamber. Along the direction from the center of the main body to the outer edge of the main body, the distance between the second quantitative chamber and the center of the main body is greater than the distance between the waste liquid chamber and the center of the main body.

[0011] Further, the reagent dispensing area includes a reagent chamber and a second processing chamber. The reagent chamber has a first injection port and is connected to the second processing chamber. The second processing chamber is connected to a first reagent metering area, a second reagent metering area, and the mixing chamber, respectively; and / or, The sample injection area includes a sample injection chamber, which has a second injection port. The sample injection chamber is connected to the sample quantification area; and / or The sample quantification area includes a separation chamber and a second waste liquid chamber. The separation chamber is connected to the sample injection area and the mixing chamber, respectively. The separation chamber is used to separate the liquid and solid in the sample under centrifugation. The second waste liquid chamber is connected to the separation chamber.

[0012] Further, the first detection area includes a first detection chamber, which is connected to both the first reagent quantitative area and the second reagent quantitative area. The first detection chamber stores a second reaction reagent. The first detection chamber is used to mix the second reaction reagent with the liquids flowing into the first detection chamber from the first and second reagent quantitative areas to form the second liquid; and / or, The second detection area includes multiple second detection chambers, all of which are connected to the buffer chamber, and each second detection chamber contains different reaction reagents.

[0013] Furthermore, the ratio of sample to reagent in the first liquid is 1:2; and / or, The ratio of the sample to the second reaction reagent in the second liquid is 1:4.

[0014] On the other hand, this application also provides a detection device, which includes the aforementioned microfluidic detection chip for multi-channel quantitative mixing.

[0015] Compared to existing technologies, this application, through the design of the first reagent quantitative region, allows the first liquid to be further diluted by the reagent, thereby preparing a mixed solution with a higher dilution ratio between the sample and the reagent. This ensures that the ratio of reagent to sample in the first and second liquids differs, allowing for the testing of blood for different purposes. This solves the problem in related technologies where microfluidic detection chips struggle to support the mixing ratios of samples and reagents for different detection methods. In some specific applications, the first liquid can be used for at least APTT, PT, TT, and FIB detection, and the second liquid can be used for at least DD detection. Attached Figure Description

[0016] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments and are used to explain this application, but do not constitute an undue limitation of this application. In the drawings: Figure 1 This is a schematic diagram of the structure of the first microfluidic detection chip for multi-channel quantitative mixing disclosed in this application; Figure 2 This is a schematic diagram of the structure of the second type of microfluidic detection chip for multi-channel quantitative mixing disclosed in this application; Figure 3 This is a schematic diagram of the working process of the first microfluidic detection chip for multi-channel quantitative mixing disclosed in this application; Figure 4 This is a schematic diagram of the workflow of the second type of microfluidic detection chip for multi-channel quantitative mixing disclosed in this application.

[0017] The above figures include the following reference numerals: 10. Reagent dispensing area; 11. Reagent chamber; 12. Second processing chamber; 20. Sample injection area; 21. Sample injection chamber; 30. First reagent quantification area; 31. First quantification chamber; 32. Storage chamber; 33. First processing chamber; 40. Second reagent quantification area; 41. Transfer chamber; 42. Third quantification chamber; 43. First waste liquid chamber; 50. First detection area; 51. First detection chamber; 60. Sample quantification area; 61. Separation chamber; 62. Second waste liquid chamber; 70. Mixing area; 71. Mixing chamber; 72. Second quantification chamber; 73. Buffer chamber; 74. Waste liquid chamber; 80. Second detection area; 81. Fourth quantification chamber; 82. Second detection chamber; 111. First injection port; 211. Second injection port; 331. First reaction reagent; 511. Second reaction reagent; 611. Supernatant chamber; 612. Sedimentation chamber; 721. First quantification chamber; 722. Second quantification chamber. Detailed Implementation

[0018] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0019] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0020] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps described in these embodiments do not limit the scope of this application. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following drawings denote similar items; therefore, once an item is defined in one drawing, it need not be further discussed in subsequent drawings.

[0021] It's worth noting that when coagulation testing is required, it involves five tests: activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), fibrinogen content (FIB), and D-dimer (DD). Specifically, APTT, PT, TT, and FIB typically use a standardized dilution ratio (e.g., sample:reagent:reaction reagent = 1:2:2), which is sufficient for routine testing. However, D-dimer testing often relies on immunoturbidimetry or latex-enhanced turbidimetry, requiring higher sensitivity and linearity. Using the standard 1:2 dilution ratio can easily lead to excessively high reagent concentrations in the reaction system, resulting in false positives and affecting disease diagnosis and clinical judgment. Therefore, D-dimer testing usually requires a higher dilution ratio (e.g., sample:reagent:reaction reagent = 1:4:4) to ensure the reliability and clinical value of the results. However, microfluidic detection chips in related technologies can usually only dilute reagents and samples to a certain ratio. This makes it difficult to detect the five coagulation items simultaneously in a single microfluidic detection chip, requiring detection at different times, which may lead to inaccurate coagulation detection of blood.

[0022] To solve the above problem, see Figures 1 to 4 As shown, according to an embodiment of this application, a detection device is provided. The detection device includes two types of microfluidic detection chips for multi-channel quantitative mixing. In the first type of microfluidic detection chip for multi-channel quantitative mixing, the microfluidic detection chip for multi-channel quantitative mixing includes a main body. The main body is provided with at least a reagent dispensing area 10, a sample injection area 20, a first reagent quantitative area 30, a second reagent quantitative area 40, a sample quantitative area 60, a mixing area 70, a first detection area 50, and a second detection area 80. The reagent dispensing area 10 is connected to the first reagent quantitative area 30, the second reagent quantitative area 40, and the mixing area 70, respectively. The sample injection area 20 is connected to the sample quantitative area 60, the sample quantitative area 60 is connected to the mixing area 70, the first reagent quantitative area 30 is also connected to the mixing area 70 and the first detection area 50, and the second detection area 80 is connected to the mixing area 70.

[0023] In this configuration, along the direction from the center of the main body to its outer edge, the distances from the reagent dispensing area 10, the second reagent quantification area 40, and the first detection area 50 to the center of the main body increase sequentially; the distances from the reagent dispensing area 10, the first reagent quantification area 30, and the first detection area 50 to the center of the main body also increase sequentially; the distances from the injection area 20, the sample quantification area 60, the mixing area 70, the first reagent quantification area 30, and the second detection area 80 to the center of the main body also increase sequentially; and the mixing area 70 is used to prepare a first liquid in a first predetermined ratio, while the first reagent quantification area 30, the second reagent quantification area 40, and the mixing area 70 work together to prepare a second liquid in a second predetermined ratio.

[0024] Specifically, the microfluidic detection chip of this application operates as follows: First, the sample is injected into the injection area 20, and the reagent is injected into the reagent dispensing area 10. Then, the microfluidic detection chip is rotated, and the sample enters the sample quantification area 60 under centrifugal force, while the reagents enter the first reagent quantification area 30, the second reagent quantification area 40, and the mixing area 70 respectively under centrifugal force. At this time, the sample and reagents in the mixing area 70 mix to form a first liquid in a first predetermined ratio. Subsequently, under centrifugal force, the first liquid in the mixing area 70 enters the first reagent quantification area 30 and the second detection area 80 respectively. The first liquid entering the second detection area 80 is used for at least the first detection, and the first liquid entering the first reagent quantification area 30 is diluted again by the reagent; simultaneously, the reagent in the second reagent quantification area 40 enters the first detection area 50 under centrifugal force. Finally, the liquid in the first reagent quantification area 30 enters the first detection area 50 under centrifugal force, thereby forming a second liquid in a second predetermined ratio, which is used for at least the second detection.

[0025] Unlike related technologies, this embodiment, through the design of the first reagent quantification region 30, allows the first liquid to be further diluted by the reagent, thereby preparing a mixed solution with a higher dilution ratio between the sample and the reagent. This results in different reagent-to-sample ratios in the first and second liquids, which can be used for different blood tests. This solves the problem in related technologies where microfluidic detection chips struggle to support different sample-to-reagent ratios for different detection projects. In some specific embodiments, the first liquid can be used for at least APTT, PT, TT, and FIB detection, and the second liquid can be used for at least DD detection.

[0026] In some embodiments, the first reagent quantification region 30 includes a first quantification chamber 31 and a first processing chamber 33. The first quantification chamber 31 is connected to the reagent dispensing region 10 via a siphon pipe, and the first processing chamber 33 is connected to the first quantification chamber 31, and is also connected to the first detection region 50 and the mixing region 70 via siphon pipes, respectively. The first processing chamber 33 stores a first reaction reagent 331. Along the direction from the center of the main body to its outer edge, the distances from the reagent dispensing region 10, the first quantification chamber 31, the mixing region 70, and the first processing chamber 33 to the center of the main body increase sequentially. The first processing chamber 33 is used to mix the first reaction reagent 331, the reagent flowing into the first processing chamber 33 from the first quantification chamber 31 and the mixing region 70, and the first liquid to form a third liquid.

[0027] Specifically, under the action of centrifugal force, the reagent in the reagent dispensing area 10 enters the first quantitative chamber 31. The first quantitative chamber 31 is used to accurately quantify a predetermined volume of reagent; that is, only a predetermined volume of reagent is retained after entering the first quantitative chamber 31. After centrifugation again, the predetermined volume of reagent in the first quantitative chamber 31 enters the first processing chamber 33 through a siphon pipe. Furthermore, the first liquid in the mixing area 70 can also enter the first processing chamber 33 through a siphon pipe under the action of centrifugal force. At this time, the first liquid entering the first processing chamber 33 is diluted again with the predetermined volume of reagent and simultaneously reacts with the first reaction reagent 331 to form a third liquid. It can be understood that when the first detection area 50 is used for DD detection of blood, since blood contains a large amount of fibrinogen, the sample needs to react with the first reaction reagent 331 to remove the fibrinogen from the sample, facilitating subsequent sample detection. In some embodiments, the first reaction reagent 331 can be polystyrene latex microparticles, magnetic microparticles, or silica microparticles, etc.

[0028] In some embodiments, the second reagent quantification region 40 further includes a storage cavity 32, which is connected to the first quantification cavity 31 and the first processing cavity 33 via siphon pipes. The distances from the first quantification cavity 31, the storage cavity 32, and the first processing cavity 33 to the center of the body increase sequentially along the direction from the center of the body to its outer edge.

[0029] Specifically, the storage chamber 32 is necessary to prevent the reagent in the first processing chamber 33 from entering the first detection area 50 without mixing with the first liquid. Without the storage chamber 32, during centrifugation, the reagent in the first processing chamber 33 would enter the first detection area 50 first, while the first liquid entering the first processing chamber 33 would not be diluted again by the reagent and would react with the first reaction reagent 331. This could result in insufficient removal of fibrinogen from the blood, affecting the detection accuracy of the microfluidic detection chip. In other words, during centrifugation, after centrifugation, the reagent enters the first quantitative chamber 31 from the reagent distribution area 10. At this time, the sample enters the mixing area 70 and mixes. After the next centrifugation, the reagent in the storage chamber 32 enters the first processing chamber 33 through a siphon pipe, and the first liquid in the mixing area 70 enters the first processing chamber 33 through a siphon pipe, thus forming a third liquid in the first processing chamber 33.

[0030] In some embodiments, the mixing region 70 includes a mixing chamber 71, a second quantitative chamber 72, and a buffer chamber 73. The mixing chamber 71 is connected to the sample quantitative region 60 and the reagent dispensing region 10 via siphon pipes, respectively. The first quantitative chamber 31 is connected to the mixing chamber 71 and the first processing chamber 33 via siphon pipes. The buffer chamber 73 is connected to the second quantitative chamber 72 via a siphon pipe and is also connected to the second detection region 80. The distances from the mixing chamber 71, the second quantitative chamber 72, the buffer chamber 73, and the second detection region 80 to the center of the body increase sequentially along the direction from the center of the body to the outer edge of the body. The mixing chamber 71 is used to mix the sample and reagent flowing into the mixing chamber 71 from the sample quantitative region 60 and the reagent dispensing region 10 respectively into a first liquid.

[0031] Specifically, when the microfluidic detection chip rotates, the sample in the sample quantification area 60 and the reagent in the reagent dispensing area 10 each enter the mixing chamber 71 through a siphon tube, where they are thoroughly mixed to form a first liquid. Subsequently, the first reagent in the mixing chamber 71, after centrifugation, enters the second quantification chamber 72. Then, a portion of the first liquid in the second quantification chamber 72 is quantified and, under centrifugation, enters the first processing chamber 33 through a siphon tube, thereby diluting the first liquid entering the first processing chamber 33 again with reagents at a predetermined ratio. Another portion of the first liquid in the second quantification chamber 72, under centrifugation, enters the buffer chamber 73 through a siphon tube. Finally, the third liquid in the first processing chamber 33, after centrifugation, enters the first detection area 50 through a siphon tube, while the first liquid in the buffer chamber 73, after centrifugation, enters the second detection area 80. This design allows at least two samples requiring simultaneous detection to enter different detection areas, facilitating the simultaneous detection of at least two items and improving the detection efficiency of the microfluidic detection chip.

[0032] In some embodiments, the second metering chamber 72 includes a first metering chamber 721, a second metering chamber 722, and a waste liquid chamber 74. The first metering chamber 721 is connected to the mixing chamber 71 and the buffer chamber 73 respectively via siphon pipes. The second metering chamber 722 is connected to the first metering chamber 721 and to the first processing chamber 33 via a siphon pipe. Along the direction from the center of the body to the outer edge of the body, the distance from the first metering chamber 721 to the center of the body is less than the distance from the second metering chamber 722 to the center of the body. The waste liquid chamber 74 is connected to the second metering chamber 722.

[0033] It is understood that, since the first metering chamber 721 and the second metering chamber 722 are connected, when the first metering chamber 721 is filled with the first liquid, excess first liquid will enter the second metering chamber 722 for metering, and excess liquid in the second metering chamber 722 will enter the waste liquid chamber 74. When the microfluidic detection chip rotates, the first liquid in the first metering chamber 721 enters the buffer chamber 73 through a siphon channel under centrifugal force, and at the same time, the first liquid in the second metering chamber 722 enters the first processing chamber 33 through a siphon channel under centrifugal force.

[0034] In some embodiments, the second reagent quantification region 40 includes a third quantification chamber 42, which is connected to the reagent dispensing region 10 and the first detection region 50 via siphon pipes. The distances from the reagent dispensing region 10, the third quantification chamber 42, and the first detection region 50 to the center of the body increase sequentially along the direction from the center of the body to its outer edge.

[0035] In this embodiment, the second reagent quantification area 40 is required so that some reagent, during the rotation of the microfluidic detection chip, first enters the first detection area under the action of centrifugal force. This allows the third liquid, after entering the first detection area from the first processing chamber 33, to be diluted again by the reagent to a predetermined proportion of the second liquid. Furthermore, the third quantification chamber 42 is used to quantify a predetermined volume of reagent, so that the predetermined volume of reagent enters the first detection area 50.

[0036] In some embodiments, the second reagent metering region 40 further includes a transfer chamber 41 and a first waste liquid chamber 43. The transfer chamber 41 is connected between the third metering chamber 42 and the reagent dispensing region 10, and the transfer chamber 41 is also connected to the first reagent metering region 30. The first waste liquid chamber 43 is connected to the third metering chamber 42, and the distances from the reagent dispensing region 10, the transfer chamber 41, the third metering chamber 42, and the first waste liquid chamber 43 to the center of the body increase sequentially along the direction from the center of the body to the outer edge of the body.

[0037] Specifically, when reagents enter the first metering chamber 31 and the third metering chamber 42 from the reagent dispensing area 10, excess reagent in the first metering chamber 31 will enter the third metering chamber 42 through the transfer chamber 41 since the first metering chamber 31 is connected to the transfer chamber 41. Simultaneously, the third metering chamber 42 is connected to the first waste liquid chamber 43, so excess reagent in the third metering chamber 42 will enter the first waste liquid chamber 43 under centrifugal force. This design avoids the problem of excess reagents in the first metering chamber 31 and the third metering chamber 42 not being discharged in time, thus preventing the problem of residual reagents in the reagent dispensing area 10. It is worth mentioning that when reagents in the reagent dispensing area 10 cannot be completely discharged, this may cause the residual reagents in the reagent dispensing area 10 to re-enter the first metering chamber 31 or the third metering chamber 42 during subsequent centrifugation of the microfluidic detection chip, ultimately leading to a change in the ratio of the second liquid.

[0038] In some embodiments, the reagent dispensing area 10 includes a reagent chamber 11 and a second processing chamber 12. The reagent chamber 11 has a first injection port 111 and is connected to the second processing chamber 12. The second processing chamber 12 is connected to the first reagent quantification area 30, the second reagent quantification area 40, and the mixing area 70, respectively. In some embodiments, the sample injection area 20 includes a sample injection chamber 21 and has a second injection port 211. The sample injection chamber 21 is connected to the sample quantification area 60.

[0039] When using a microfluidic detection chip for detection, reagents are first injected into the reagent chamber 11 through the first injection port 111, while samples are injected into the sample injection chamber 21 through the second injection port 211. Subsequently, the microfluidic detection chip is rotated. After rotation, the reagents in the reagent chamber 11 enter the second processing chamber 12 under centrifugal force, while the samples in the sample injection chamber 21 enter the sample quantification region 60 under centrifugal force. Furthermore, after entering the second processing chamber 12, the reagents again enter the first reagent quantification region 30, the second reagent quantification region 40, and the mixing region 70 under centrifugal force. Simultaneously, the samples in the sample quantification region 60 enter the mixing region 70 under centrifugal force and mix with the reagents.

[0040] In some embodiments, the sample quantification region 60 includes a separation chamber 61 and a second waste liquid chamber 62. The separation chamber 61 is connected to the sample injection region 20 and the mixing region 70, respectively. The separation chamber 61 is used to separate the liquid and solid in the sample under centrifugation. The second waste liquid chamber 62 is connected to the separation chamber 61.

[0041] Specifically, the separation chamber 61 includes a supernatant chamber 611 and a sedimentation chamber 612. Along the direction from the center of the main body to its outer edge, the distance from the center of the sedimentation chamber 612 to the center of the main body is greater than the distance from the center of the supernatant chamber 611. When the microfluidic detection chip rotates, the sample is separated into plasma and blood cells under centrifugal force. Blood cells enter the sedimentation chamber 612, while plasma remains in the supernatant chamber 611. A siphon channel is provided between the supernatant chamber 611 and the sedimentation chamber 612. This siphon channel communicates with the mixing region 70, allowing plasma in the supernatant chamber 611 to enter the mixing region 70 through the siphon channel, preventing blood cells from entering the mixing region 70 and affecting the detection accuracy of the microfluidic detection chip. Furthermore, the design of the second waste liquid chamber 62 enables the supernatant chamber 611 to have a quantitative function. That is, during centrifugation, excess plasma enters the second waste liquid chamber 62 instead of entering the mixing region 70 through the siphon channel, preventing changes in the sample-to-reagent ratio in the first liquid.

[0042] In some embodiments, the first detection region 50 includes a first detection chamber 51, which is connected to a first reagent quantitative region 30 and a second reagent quantitative region 40. The first detection chamber 51 stores a second reaction reagent 551 and is used to mix the second reaction reagent 551 with the liquids flowing into it from the first reagent quantitative region 30 and the second reagent quantitative region 40 to form a second liquid. In this embodiment, the first detection chamber 51 is used for DD detection of blood. Since the first liquid is diluted and reacted in the first processing chamber 33, a third liquid is formed within the first processing chamber 33. To improve the accuracy of DD detection, the third liquid needs to enter the first detection chamber 51 and react again with the second reaction reagent 551 to generate a detectable turbidity signal, thereby improving the accuracy of DD detection. In this embodiment, the second reaction reagent 551 can be polystyrene latex microparticles or mouse anti-human D-dimer monoclonal antibody, etc.

[0043] In some embodiments, the second detection region 80 includes a plurality of second detection cavities 82, all of which are connected to the mixing region 70, and each second detection cavity 82 contains a different reaction reagent.

[0044] In one specific embodiment, the second detection chamber 82 includes four chambers, each used for APTT, PT, TT, and FIB detection, and each chamber contains the corresponding reaction reagent required for the detection. It is understood that since the sample-to-reagent ratios required for APTT, PT, TT, and FIB detections are all the same, this embodiment can simultaneously detect APTT, PT, TT, and FIB in blood in a single test. In some embodiments, the second detection region 80 further includes a fourth quantitative chamber 81, which is disposed between the second detection chamber 82 and the mixing region 70. The second quantitative chamber 82 allows a fixed volume of first liquid to enter the second detection chamber 82 and mix with the reaction reagents in the second detection chamber 82 to form a solution in a predetermined ratio. Multiple fourth quantitative chambers 81 are provided, each corresponding to one of the multiple second detection chambers 82.

[0045] In some embodiments, the sample to reagent ratio in the first liquid is 1:2. Specifically, in this embodiment, the volume ratio of the supernatant chamber to the second processing chamber 12 is 1:2. Therefore, when the sample in the supernatant and the reagent in the second processing chamber 12 both enter the mixing chamber 71, the sample and reagent can form a first liquid with a 1:2 ratio for use in the detection of APTT, PT, TT, and FIB in blood.

[0046] In some embodiments, the ratio of sample to second reaction reagent 551 in the second liquid is 1:4. Specifically, in some embodiments, the ratio of sample to reagent in the second liquid is 1:4; of course, in other embodiments, the ratio of sample to reagent in the second liquid can also be 1:5, 1:6, or a higher ratio. In this application, it is only necessary to ensure that the ratio of sample to second reaction reagent 551 in the second liquid is 1:4 in order to improve the accuracy of blood DD detection.

[0047] Specifically, the working process of the first microfluidic detection chip in this application is as follows: Step 1: Inject reagent into reagent chamber 11 and inject sample into injection chamber 21.

[0048] Step 2: Rotate the microfluidic detection chip. Under the action of centrifugal force, the reagent in reagent chamber 11 enters the second processing chamber, and part of the reagent in the second processing chamber enters the first quantitative chamber 31, the third quantitative chamber 42 and the first waste liquid chamber 43 in sequence. At the same time, the sample in the sample injection chamber 21 enters the separation chamber 61 and is separated into plasma and blood cells under the action of centrifugal force. The blood cells enter the sedimentation chamber 612, the plasma is located in the supernatant chamber 611, and the excess plasma enters the second waste liquid chamber 62.

[0049] Step 3: Centrifuge the microfluidic detection chip again. The plasma in the supernatant chamber 611 enters the mixing chamber 71 through the siphon channel, while the reagent in the second processing chamber 12 enters the mixing chamber 71 through the siphon channel and mixes with the plasma to form the first liquid. At the same time, the reagent in the first quantitative chamber 31 enters the storage chamber 32 through the siphon channel, and the reagent in the third quantitative chamber 42 enters the first detection chamber 51 through the siphon channel and mixes with the second reaction reagent 551.

[0050] Step 4: Centrifuge the microfluidic detection chip again. The first liquid in the mixing chamber 71 enters the first quantitative chamber 721 through the siphon tube. The excess first liquid in the first quantitative chamber 721 flows into the second quantitative chamber 722, and the excess first liquid in the second quantitative chamber 722 flows into the waste liquid chamber 74. At the same time, the reagent in the storage chamber 32 enters the first processing chamber through the siphon channel.

[0051] Step 5: Centrifuge the microfluidic detection chip again. The first liquid in the first quantitative chamber 721 enters the buffer chamber 73 through the siphon channel and finally enters the fourth quantitative chamber 81. The first liquid in the second quantitative chamber 722 enters the first processing chamber through the siphon channel and mixes with the reagent and the second reaction reagent 551 to form the third liquid.

[0052] Step 6: Centrifuge the microfluidic detection chip again. The first liquid in the fourth quantitative chamber 81 enters the second detection chamber 82. At the same time, the third liquid enters the first detection chamber 51 through the siphon tube and forms the second liquid with the reagent and the second reaction reagent 551.

[0053] In the second type of microfluidic detection chip, the microfluidic detection chip includes a main body, on which at least a reagent dispensing area 10, a sample injection area 20, a first reagent quantification area 30, a second reagent quantification area 40, a sample quantification area 60, a mixing chamber 71, a buffer chamber 73, a second quantification chamber 72, a first detection area 50, and a second detection area 80 are provided. The reagent dispensing area 10 is connected to the first reagent quantification area 30, the second reagent quantification area 40, and the mixing chamber 71, respectively. The sample injection area 20 is connected to the sample quantification area 60, the sample quantification area 60 is connected to the mixing chamber 71, the buffer chamber 73 is connected to the mixing chamber 71, the second quantification chamber 72 is connected to the buffer chamber 73, the first reagent quantification area 30 is also connected to the second quantification chamber 72 and the first detection area 50, and the second detection area 80 is connected to the buffer chamber 73.

[0054] In this configuration, along the direction from the center of the main body to its outer edge, the distances from the reagent dispensing area 10, the second reagent quantitative area 40, and the first detection area 50 to the center of the main body increase sequentially; the distances from the reagent dispensing area 10, the first reagent quantitative area 30, and the first detection area 50 to the center of the main body also increase sequentially; the distances from the sample injection area 20, the sample quantitative area 60, the mixing chamber 71, the buffer chamber 73, the second quantitative chamber 72, the first reagent quantitative area 30, and the second detection area 80 to the center of the main body also increase sequentially. The mixing chamber 71 is used to prepare a first liquid in a first predetermined ratio, and the first reagent quantitative area 30, the second reagent quantitative area 40, the second quantitative chamber 72, and the first detection area 50 work together to prepare a second liquid in a second predetermined ratio.

[0055] Specifically, the working process of the microfluidic detection chip of this application is as follows: First, reagent is injected into the reagent dispensing area 10, and sample is injected into the sample injection area 20. Then, the microfluidic detection chip is rotated, and the reagent in the reagent dispensing area 10 enters the mixing chamber 71, the first reagent quantification area 30, and the second reagent quantification area 40 respectively under centrifugal force. Simultaneously, the sample in the sample injection area enters the mixing chamber 71 under centrifugal force. The sample entering the mixing chamber 71 mixes with the reagent to form a first liquid in a first predetermined ratio. Afterwards, the first liquid in the mixing chamber 71 enters the buffer chamber 73 under centrifugal force, and a portion of the first liquid enters the second quantification chamber 72. Simultaneously, the reagent in the first reagent quantification area enters the first detection area 50. After the microfluidic detection chip is centrifuged again, the first reagent in the buffer chamber 73 enters the second detection area 80, and the first liquid in the second quantification chamber 72 enters the first reagent quantification area 30 under centrifugal force, and mixes again with the reagent in the first reagent quantification area 30. Finally, the liquid in the first reagent quantification area 30 enters the first detection area 50 under the action of centrifugal force and forms the second liquid.

[0056] Unlike related technologies, this embodiment, through the design of the first reagent quantification region 30, allows the first liquid to be further diluted by the reagent, thereby preparing a mixed solution with a higher dilution ratio between the sample and the reagent. This results in different reagent-to-sample ratios in the first and second liquids, which can be used for different blood tests. This solves the problem in related technologies where microfluidic detection chips struggle to support different sample-to-reagent ratios for different detection projects. In some specific embodiments, the first liquid can be used for at least APTT, PT, TT, and FIB detection, and the second liquid can be used for at least DD detection.

[0057] In some embodiments, the first reagent quantification region 30 includes a first quantification chamber 31 and a first processing chamber 33. The first quantification chamber 31 is connected to the reagent dispensing region 10 via a siphon pipe. The first processing chamber 33 is connected to the first quantification chamber 31, and is also connected to the first detection region 50 and the second quantification chamber 72 via siphon pipes. The first processing chamber 33 contains a first reaction reagent 331. Along the direction from the center of the main body to its outer edge, the distances from the reagent dispensing region 10, the first quantification chamber 31, the second quantification chamber 72, and the first processing chamber 33 to the center of the main body increase sequentially. The first processing chamber 33 is used to mix the first reaction reagent 331, the reagent flowing into the first processing chamber 33 from the first quantification chamber 31 and the second quantification chamber 72, and the first liquid to form a third liquid.

[0058] Specifically, under the action of centrifugal force, the reagent in the reagent dispensing area 10 enters the first quantitative chamber 31. The first quantitative chamber 31 is used to accurately quantify a predetermined volume of reagent; that is, only a predetermined volume of reagent is retained after entering the first quantitative chamber 31. After centrifugation again, the predetermined volume of reagent in the first quantitative chamber 31 enters the first processing chamber 33 through a siphon pipe. Furthermore, the first liquid in the second quantitative chamber 72 can also enter the first processing chamber 33 through a siphon pipe under the action of centrifugal force. At this time, the first liquid entering the first processing chamber 33 is diluted again with the predetermined volume of reagent and simultaneously reacts with the first reaction reagent 331 to form a third liquid. It can be understood that when the first detection area 50 is used for DD detection of blood, since blood contains a large amount of fibrinogen, the sample needs to react with the first reaction reagent 331 to remove the fibrinogen from the sample, facilitating subsequent sample detection. In some embodiments, the first reaction reagent 331 can be polystyrene latex microparticles, magnetic microparticles, or silica microparticles, etc.

[0059] In some embodiments, the second reagent quantification region 40 further includes a storage cavity 32, which is connected to the first quantification cavity 31 and the first processing cavity 33 via siphon pipes. The distances from the first quantification cavity 31, the storage cavity 32, and the first processing cavity 33 to the center of the body increase sequentially along the direction from the center of the body to its outer edge.

[0060] Similar to the first type of microfluidic detection chip, the storage chamber 32 is necessary to prevent the reagent in the first processing chamber 33 from entering the first detection area 50 without mixing with the first liquid. Without the storage chamber 32, during centrifugation, the reagent in the first processing chamber 33 would enter the first detection area 50 first, and the first liquid entering the first processing chamber 33 would not be diluted again by the reagent, reacting with the first reaction reagent 331. This could result in insufficient removal of fibrinogen from the blood, affecting the detection accuracy of the microfluidic detection chip. In other words, during centrifugation, after centrifugation, the reagent enters the first quantitative chamber 31 from the reagent distribution area 10, while the first liquid formed in the mixing chamber 71 enters the second quantitative chamber 72. After the next centrifugation, the reagent in the storage chamber 32 enters the first processing chamber 33 through a siphon pipe, and the first liquid in the second quantitative chamber 72 enters the first processing chamber 33 through a siphon pipe, thus forming a third liquid in the first processing chamber 33.

[0061] In some embodiments, the second reagent quantification region 40 includes a third quantification chamber 42, which is connected to the reagent dispensing region 10 and the first detection region 50 via siphon pipes. The distances from the reagent dispensing region 10, the third quantification chamber 42, and the first detection region 50 to the center of the body increase sequentially along the direction from the center of the body to its outer edge.

[0062] Similarly, the second reagent quantification zone is needed so that some reagent, during the rotation of the microfluidic detection chip, first enters the first detection zone under the action of centrifugal force. This allows the third liquid, after entering the first detection zone from the first processing chamber 33, to be diluted again by the reagent to a predetermined proportion of the second liquid. Furthermore, the third quantification chamber 42 is used to quantify a predetermined volume of reagent, so that the predetermined volume of reagent enters the first detection area 50.

[0063] In some embodiments, the second reagent metering region 40 further includes a transfer chamber 41 and a first waste liquid chamber 43. The transfer chamber 41 is connected between the third metering chamber 42 and the reagent dispensing region 10, and the transfer chamber 41 is also connected to the first reagent metering region 30. The first waste liquid chamber 43 is connected to the third metering chamber 42, and the distances from the reagent dispensing region 10, the transfer chamber 41, the third metering chamber 42, and the first waste liquid chamber 43 to the center of the body increase sequentially along the direction from the center of the body to the outer edge of the body.

[0064] Similarly, when reagents enter the first metering chamber 31 and the third metering chamber 42 from the reagent dispensing area 10, excess reagent in the first metering chamber 31 will enter the third metering chamber 42 through the transfer chamber 41 since the first metering chamber 31 is connected to the transfer chamber 41. Simultaneously, the third metering chamber 42 is connected to the first waste liquid chamber 43, so excess reagent in the third metering chamber 42 will enter the first waste liquid chamber 43 under centrifugal force. This design avoids the problem of excess reagents in the first metering chamber 31 and the third metering chamber 42 not being discharged in time, thus preventing the presence of residual reagents in the reagent dispensing area 10. It is worth mentioning that if the reagents in the reagent dispensing area 10 cannot be completely discharged, this may cause the residual reagents in the reagent dispensing area 10 to re-enter the first metering chamber 31 or the third metering chamber 42 during subsequent centrifugation of the microfluidic detection chip, ultimately leading to a change in the ratio of the second liquid.

[0065] In some embodiments, the microfluidic detection chip further includes a waste liquid chamber 74, which is connected to a second metering chamber 72 and is located along the direction from the center of the body to the outer edge of the body. The distance between the second metering chamber 72 and the center of the body is greater than the distance between the waste liquid chamber 74 and the center of the body.

[0066] It is understandable that to form the predetermined ratio of the third liquid, it is necessary to strictly control the volume of the first liquid entering the first processing chamber, as well as the volume of the reagent entering the first processing chamber. Therefore, in this embodiment, a predetermined volume of the first liquid is quantitatively introduced into the first processing chamber through the second metering chamber 72, while excess first liquid enters the waste liquid chamber 74 under the action of centrifugal force, thus preventing excessive first liquid from entering the first processing chamber 33 through the second metering chamber 72.

[0067] In some embodiments, the reagent dispensing area 10 includes a reagent chamber 11 and a second processing chamber 12. The reagent chamber 11 has a first injection port 111 and is connected to the second processing chamber 12. The second processing chamber 12 is connected to the first reagent quantification area 30, the second reagent quantification area 40, and the mixing chamber 71, respectively. In some embodiments, the sample injection area 20 includes a sample injection chamber 21 and has a second injection port 211. The sample injection chamber 21 is connected to the sample quantification area 60.

[0068] When using a microfluidic detection chip for detection, reagents are first injected into the reagent chamber 11 through the first main inlet, while samples are injected into the sample inlet 21 through the second main inlet. Subsequently, the microfluidic detection chip is rotated. After rotation, the reagents in the reagent chamber 11 enter the first processing chamber 33 under centrifugal force, while the samples in the sample inlet 21 enter the sample quantification region 60 under centrifugal force. Furthermore, after entering the second processing chamber 12, the reagents again enter the first reagent quantification region 30, the second reagent quantification region 40, and the mixing chamber 71 under centrifugal force. Simultaneously, the samples in the sample quantification region 60 enter the mixing chamber 71 under centrifugal force and mix with the reagents.

[0069] In some embodiments, the sample quantification region 60 includes a separation chamber 61 and a second waste liquid chamber 62. The separation chamber 61 is connected to the sample injection region 20 and the mixing chamber 71, respectively. The separation chamber 61 is used to separate the liquid and solid in the sample under centrifugation. The second waste liquid chamber 62 is connected to the separation chamber 61.

[0070] Specifically, the separation chamber 61 includes a supernatant chamber 611 and a sedimentation chamber 612. Along the direction from the center of the main body to its outer edge, the distance from the center of the sedimentation chamber 612 to the center of the main body is greater than the distance from the center of the supernatant chamber 611. When the microfluidic detection chip rotates, the sample is separated into plasma and blood cells under centrifugal force. Blood cells enter the sedimentation chamber 612, while plasma remains in the supernatant chamber 611. A siphon channel is provided between the supernatant chamber 611 and the sedimentation chamber 612. This siphon channel communicates with the mixing chamber 71, allowing plasma in the supernatant chamber 611 to enter the mixing chamber 71 through the siphon channel, preventing blood cells from entering the mixing chamber 71 and affecting the detection accuracy of the microfluidic detection chip. Furthermore, the design of the second waste liquid chamber 62 enables the supernatant chamber 611 to have a quantitative function. That is, during centrifugation, excess plasma enters the second waste liquid chamber 62 instead of entering the mixing chamber 71 through the siphon channel, preventing changes in the sample-to-reagent ratio in the first liquid.

[0071] In some embodiments, the first detection region 50 includes a first detection chamber 51, which is connected to a first reagent quantitative region 30 and a second reagent quantitative region 40. The first detection chamber 51 stores a second reaction reagent 551 and is used to mix the second reaction reagent 551 with the liquids flowing into it from the first reagent quantitative region 30 and the second reagent quantitative region 40 to form a second liquid. Similarly, in this embodiment, the first detection chamber 51 is used for DD detection of blood. Since the first liquid is diluted and reacted in the first processing chamber 33, a third liquid is formed in the first processing chamber 33. To improve the accuracy of DD detection, the third liquid needs to enter the first detection chamber 51 and react again with the second reaction reagent 551 to generate a detectable turbidity signal, thereby improving the accuracy of DD detection. In this embodiment, the second reaction reagent 551 can be polystyrene latex microparticles or mouse anti-human D-dimer monoclonal antibody, etc.

[0072] In some embodiments, the second detection region 80 includes a plurality of second detection cavities 82, all of which are connected to the buffer cavity 73, and each second detection cavity 82 contains a different reaction reagent.

[0073] In one specific embodiment, the second detection chamber 82 includes four chambers, each used for APTT, PT, TT, and FIB detection, and each chamber contains the corresponding reaction reagent required for the detection. It is understood that since the sample-to-reagent ratios required for APTT, PT, TT, and FIB detections are all the same, this embodiment can simultaneously perform APTT, PT, TT, and FIB detections on blood in a single test. In some embodiments, the second detection area 80 further includes a fourth quantitative chamber 81, which is disposed between the second detection chamber 82 and the buffer chamber 73. The second quantitative chamber 72 allows a fixed volume of first liquid to enter the second detection chamber 82 and mix with the reaction reagents in the second detection chamber 82 to form a solution in a predetermined ratio. Multiple fourth quantitative chambers 81 are provided, each corresponding to one of the multiple second detection chambers 82.

[0074] In some embodiments, the sample to reagent ratio in the first liquid is 1:2. Specifically, in this embodiment, the volume ratio of the supernatant chamber to the second processing chamber 12 is 1:2. Therefore, when the sample in the supernatant and the reagent in the second processing chamber 12 both enter the mixing chamber 71, the sample and reagent can form a first liquid with a 1:2 ratio for use in the detection of APTT, PT, TT, and FIB in blood.

[0075] In some embodiments, the ratio of sample to second reaction reagent 551 in the second liquid is 1:4. Specifically, in some embodiments, the ratio of sample to reagent in the second liquid is 1:4; of course, in other embodiments, the ratio of sample to reagent in the second liquid can also be 1:5, 1:6, or a higher ratio. In this application, it is only necessary to ensure that the ratio of sample to second reaction reagent 551 in the second liquid is 1:4 in order to improve the accuracy of blood DD detection.

[0076] Specifically, the working process of the second type of microfluidic detection chip in this application is as follows: Step 1: Inject reagent into reagent chamber 11 and inject sample into injection chamber 21.

[0077] Step 2: Rotate the microfluidic detection chip. Under centrifugal force, the reagent in reagent chamber 11 enters the second processing chamber, and a portion of the reagent in the second processing chamber sequentially enters the first quantitative chamber 31, the third quantitative chamber 42, and the first waste liquid chamber 43. Simultaneously, the sample in injection chamber 21 enters separation chamber 61 and is separated into plasma and blood cells under centrifugal force. Blood cells enter sedimentation chamber 612, plasma is located in supernatant chamber 611, and excess plasma enters the second waste liquid chamber 62.

[0078] Step 3: Centrifuge the microfluidic detection chip again. The plasma in the supernatant chamber 611 enters the mixing chamber 71 through the siphon channel, while the reagent in the second processing chamber 12 enters the mixing chamber 71 through the siphon channel and mixes with the plasma to form the first liquid. At the same time, the reagent in the first quantitative chamber 31 enters the storage chamber 32 through the siphon channel, and the reagent in the third quantitative chamber 42 enters the first detection chamber 51 through the siphon channel and mixes with the second reaction reagent 551.

[0079] Step 4: Centrifuge the microfluidic detection chip again. The first liquid in the mixing chamber 71 enters the buffer chamber 73 through the siphon tube. Part of the first liquid in the buffer chamber 73 enters the second quantitative chamber 72. Part of the second liquid in the buffer chamber 73 enters the fourth quantitative chamber 81. Meanwhile, part of the first liquid in the second quantitative chamber 72 enters the waste liquid chamber 74. At the same time, the reagent in the storage chamber 32 enters the first processing chamber through the siphon channel.

[0080] Step 5: Centrifuge the microfluidic detection chip again. The first liquid in the second quantitative chamber 72 enters the first processing chamber through the siphon channel and mixes with the reagent and the second reaction reagent 551 to form the third liquid. At the same time, the first reagent in the fourth quantitative chamber 81 enters the second detection chamber 82.

[0081] Step 6: Centrifuge the microfluidic detection chip again. The third liquid enters the first detection chamber 51 through the siphon tube and forms a second liquid with the reagent and the second reaction reagent 551.

[0082] In this application, the second type of microfluidic detection chip differs from the first type in that it does not require a first quantitative chamber 721. The second quantitative chamber 722 of the first type of microfluidic detection chip is equivalent to the second quantitative cavity 72 of the second type, but the connection positions are different. The second quantitative chamber 722 of the first type of microfluidic detection chip is connected to the first quantitative chamber 721, while the second quantitative cavity 72 of the second type of microfluidic detection chip is connected to the buffer cavity 73. Compared with the first type of microfluidic detection chip, the second type of microfluidic detection chip has a simpler structure, fewer chambers, and lower manufacturing costs. The first type of microfluidic detection chip allows the first liquid to enter the second detection area 80 while the third liquid enters the first detection area 50 simultaneously. This means that the first type of microfluidic detection chip can simultaneously detect the five coagulation parameters of blood, and its detection accuracy is relatively higher than that of the second type of microfluidic detection chip.

[0083] For ease of description, spatial relative terms such as "above," "on top of," "on the upper surface of," "above," etc., are used herein to describe the spatial positional relationship of a device or feature as shown in the figures to other devices or features. It should be understood that spatial relative terms are intended to encompass different orientations in use or operation beyond the orientation of the device as described in the figures. For example, if the device in the figures were inverted, a device described as "above" or "on top of" other devices or structures would subsequently be positioned as "below" or "under" other devices or structures. Thus, the exemplary term "above" can include both "above" and "below." The device may also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatial relative descriptions used herein will be interpreted accordingly.

[0084] Furthermore, it should be noted that the use of terms such as "first" and "second" to define components is merely for the purpose of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore cannot be construed as limiting the scope of protection of this application.

[0085] The above are merely preferred embodiments of this application and are not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A microfluidic detection chip for multi-channel quantitative mixing, characterized in that, include: The main body is provided with at least a reagent dispensing area (10), an injection area (20), a first reagent quantification area (30), a second reagent quantification area (40), a sample quantification area (60), a mixing chamber (71), a buffer chamber (73), a second quantification chamber (72), a first detection area (50), and a second detection area (80). The reagent dispensing area (10) is connected to the first reagent quantification area (30), the second reagent quantification area (40), and the mixing chamber (71), respectively. The injection area (20) is connected to the sample quantification area (60). The sample quantification area (60) is connected to the mixing chamber (71). The buffer chamber (73) is connected to the mixing chamber (71). The second quantification chamber (72) is connected to the buffer chamber (73). The first reagent quantification area (30) is also connected to the second quantification chamber (72) and the first detection area (50). The second detection area (80) is connected to the buffer chamber (73). Wherein, along the direction from the center of the main body to the outer edge of the main body, the distances from the reagent dispensing area (10), the second reagent quantification area (40), and the first detection area (50) to the center of the main body increase sequentially, the distances from the reagent dispensing area (10), the first reagent quantification area (30), and the first detection area (50) to the center of the main body increase sequentially, the distances from the sample injection area (20), the sample quantification area (60), the mixing chamber (71), the buffer chamber (73), the second quantification chamber (72), the first reagent quantification area (30), and the second detection area (80) to the center of the main body increase sequentially, and the mixing chamber (71) is used to mix a first liquid in a first predetermined ratio, and the first reagent quantification area (30), the second reagent quantification area (40), the second quantification chamber (72), and the first detection area (50) work together to mix a second liquid in a second predetermined ratio.

2. The microfluidic detection chip for multi-channel quantitative mixing according to claim 1, characterized in that, The first reagent quantification region (30) includes: The first quantitative chamber (31) is connected to the reagent dispensing area (10) via a siphon pipe; The first processing chamber (33) is connected to the first quantitative chamber (31), and the first processing chamber (33) is connected to the first detection area (50) and the second quantitative chamber (72) respectively through a siphon pipe. The first processing chamber (33) contains a first reaction reagent (331). Wherein, along the direction from the center of the main body to the outer edge of the main body, the distances from the reagent dispensing area (10), the first quantitative cavity (31), the second quantitative cavity (72) and the first processing cavity (33) to the center of the main body increase sequentially, and the first processing cavity (33) is used to mix the first reaction reagent (331) and the reagents flowing into the first processing cavity (33) from the first quantitative cavity (31) and the second quantitative cavity (72) respectively with the first liquid to form a third liquid.

3. The microfluidic detection chip for multi-channel quantitative mixing according to claim 2, characterized in that, The second reagent quantification area (40) further includes a storage chamber (32), which is connected to the first quantification chamber (31) and the first processing chamber (33) respectively via siphon pipes; In particular, along the direction from the center of the main body to the outer edge of the main body, the distances from the first quantitative cavity (31), the storage cavity (32), and the first processing cavity (33) to the center of the main body increase sequentially.

4. The microfluidic detection chip for multi-channel quantitative mixing according to claim 1, characterized in that, The second reagent quantification area (40) includes a third quantification chamber (42), which is connected to the reagent dispensing area (10) and the first detection area (50) respectively through a siphon pipe; In particular, along the direction from the center of the main body to the outer edge of the main body, the distances from the reagent dispensing area (10), the third quantitative cavity (42), and the first detection area (50) to the center of the main body increase sequentially.

5. The microfluidic detection chip for multi-channel quantitative mixing according to claim 4, characterized in that, The second reagent quantification region (40) also includes: The transfer chamber (41) is connected between the third quantitative chamber (42) and the reagent dispensing area (10), and the transfer chamber (41) is connected to the first reagent quantitative area (30); The first waste liquid chamber (43) is connected to the third quantitative chamber (42), and the distances from the reagent dispensing area (10), the transfer chamber (41), the third quantitative chamber (42) and the first waste liquid chamber (43) to the center of the main body increase sequentially along the direction from the center of the main body to the outer edge of the main body.

6. The microfluidic detection chip for multi-channel quantitative mixing according to any one of claims 1 to 5, characterized in that, The microfluidic detection chip for multi-channel quantitative mixing also includes a waste liquid chamber (74), which is connected to the second quantitative chamber (72) and is located along the direction from the center of the main body to the outer edge of the main body. The distance between the second quantitative chamber (72) and the center of the main body is greater than the distance between the waste liquid chamber (74) and the center of the main body.

7. The microfluidic detection chip for multi-channel quantitative mixing according to any one of claims 1 to 5, characterized in that, The reagent dispensing area (10) includes a reagent chamber (11) and a second processing chamber (12). The reagent chamber (11) has a first injection port (111). The reagent chamber (11) is connected to the second processing chamber (12). The second processing chamber (12) is connected to a first reagent quantification area (30), a second reagent quantification area (40), and the mixing chamber (71), respectively; and / or, The sample injection area (20) includes a sample injection chamber (21), on which a second injection port (211) is provided. The sample injection chamber (21) is connected to the sample quantification area (60); and / or, The sample quantification area (60) includes a separation chamber (61) and a second waste liquid chamber (62). The separation chamber (61) is connected to the sample injection area (20) and the mixing chamber (71) respectively. The separation chamber (61) is used to separate the liquid and solid in the sample under centrifugation. The second waste liquid chamber (62) is connected to the separation chamber (61).

8. The microfluidic detection chip for multi-channel quantitative mixing according to any one of claims 1 to 5, characterized in that, The first detection area (50) includes a first detection chamber (51), which is connected to the first reagent quantitative area (30) and the second reagent quantitative area (40) respectively. The first detection chamber (51) stores a second reaction reagent (551). The first detection chamber (51) is used to mix the second reaction reagent (551) with the liquids flowing into the first detection chamber (51) from the first reagent quantitative area (30) and the second reagent quantitative area (40) respectively to form the second liquid; and / or, The second detection area (80) includes multiple second detection chambers (82), all of which are connected to the buffer chamber (73), and each second detection chamber (82) contains different reaction reagents.

9. The microfluidic detection chip for multi-channel quantitative mixing according to claim 8, characterized in that, The ratio of sample to reagent in the first liquid is 1:2; and / or, The ratio of the sample in the second liquid to the second reaction reagent (551) is 1:

4.

10. A testing device, characterized in that, The detection device includes the microfluidic detection chip for multi-channel quantitative mixing as described in any one of claims 1 to 9.

Images