Polyphosphorus bacteria and application thereof in phosphorus removal in aquaculture water body

Abstract

The application discloses a polyphosphorus subatlantic bacillus and application thereof, the polyphosphorus subatlantic bacillus 1-29 is identified as subatlantic bacillus ( Atlantibacter sp. ), is preserved in China Microbial Culture Collection Center (CGMCC), and the preservation number is CGMCC No.37506, and the preservation date is January 22, 2026.The polyphosphorus bacteria subatlantic bacillus 1-29 ( Atlantibacter sp. 1-29 ) provided in the application has a better phosphorus removal effect under the conditions that pH is 6-9, initial phosphorus concentration is below 4 mg / L and temperature is 15-30 DEG C, is above 85%;has a higher phosphorus removal potential in actual aquaculture water in-situ purification; and the strain has high safety, and has good application value in aquaculture tail water treatment.

Description

Technical Field

[0001] This invention relates to aquaculture water purification technology, specifically to a polyphosphate-accumulating bacteria for in-situ purification of freshwater aquaculture water and its application. Background Technology

[0002] As aquaculture develops towards intensification, factory farming, and high density, problems such as excessive feeding and low feed utilization are common. Unutilized feed residue and animal excrement continuously introduce nutrients into the water, leading to the gradual accumulation of nutrients such as phosphorus. Phosphorus, a significant limiting factor for eutrophication, can easily cause excessive algal blooms when its concentration rises, resulting in decreased water transparency, increased dissolved oxygen fluctuations, and water quality deterioration. This not only affects the normal growth of aquatic animals but may also adversely impact the surrounding aquatic environment through wastewater discharge.

[0003] Currently, the main methods for controlling and removing phosphorus pollution in water bodies include physical, chemical, and biological methods. Physical methods, such as adsorption and filtration, are relatively simple to operate, but the adsorption materials are expensive, prone to saturation and failure, and the subsequent regeneration or disposal processes are complex. Chemical methods usually remove phosphorus by adding chemical agents such as metal salts to cause phosphorus to precipitate, but this method has problems such as high agent consumption, high operating costs, easy generation of secondary pollution, and potential adverse effects on aquaculture organisms, making it difficult to apply stably in aquaculture water bodies in the long term.

[0004] Biological methods have gradually become a hot topic in water phosphorus removal research due to their environmental friendliness and low operating costs. Among them, polyphosphate-accumulating bacteria (PABs) can actively absorb orthophosphate from water through their metabolic activities and store it within their cells in the form of polyphosphate, thereby achieving phosphorus removal from water. Currently, PPA-related technologies are mainly applied in urban wastewater treatment systems or the treatment of high-concentration phosphorus-containing wastewater, typically relying on specific process conditions or complex operation and management models.

[0005] However, aquaculture water bodies are characterized by low phosphorus concentrations, high openness, high dissolved oxygen levels, and limited operational and management conditions, making existing polyphosphate-accumulating bacteria (PABs) and their application technologies insufficient for these water bodies. On the one hand, PPBs from different sources vary significantly in their polyphosphate accumulation capacity, environmental adaptability, and stability. While some strains exhibit certain phosphorus removal effects, they struggle to maintain stable performance in actual aquaculture environments over the long term. On the other hand, microorganisms in aquaculture water bodies come into direct contact with farmed animals, requiring functional strains for in-situ water purification to possess high biosafety. However, existing reports on the safety evaluation of PPBs are still relatively limited.

[0006] Therefore, it is still necessary to screen and obtain a microbial strain that is suitable for in-situ purification of aquaculture water, has strong phosphorus accumulation capacity, good environmental adaptability and high biosafety, in order to meet the actual needs of phosphorus pollution control in aquaculture water. Summary of the Invention

[0007] Purpose of the invention: To address the problem that low-concentration phosphorus pollution in aquaculture water is difficult to remove in situ safely and stably under existing technological conditions, this invention aims to provide polyphosphate-accumulating bacteria suitable for safe in-situ purification of low-concentration phosphorus in aquaculture water, as well as a method for applying these polyphosphate-accumulating bacteria to remove phosphorus in aquaculture water, ensuring the safety of aquatic animals during the application of these polyphosphate-accumulating bacteria.

[0008] Technical solution: The polyphosphate-ladenella Atlanta bacillus 1-29 described in this invention has been identified as Atlanta bacillus ( Atlantibacter sp. It was deposited at the China General Microbiological Culture Collection Center (CGMCC) on January 22, 2026, with accession number CGMCC No. 37506.

[0009] The 16S rDNA gene sequence of the *Polyphosate Atlanta bacillus* 1-29 is shown in SEQ ID No. 1.

[0010] The application of Polyphosphatobacter Atlanta 1-29 in phosphorus removal in aquaculture water.

[0011] The aforementioned applications refer to aquaculture water bodies including pond aquaculture systems, recirculating aquaculture systems, or cage aquaculture systems.

[0012] In the aforementioned application, the aquaculture water has a pH of 6.0–9.0, a temperature of 15–30 ℃, and an initial phosphorus concentration of 0.5–4.0 mg / L.

[0013] An agent or solution of bacteria comprising the aforementioned Polyphosphorus Atlanta bacillus 1-29.

[0014] The application of the aforementioned bacterial agent or bacterial solution in phosphorus removal in aquaculture water.

[0015] The method for phosphorus removal from phosphorus-containing water in aquaculture using *Polyphosate Atlantaobacter 1-29* includes the following steps: (1) The *Polyphosate Atlanta bacillus* 1-29 was cultured to the logarithmic growth phase to prepare a bacterial culture; (2) Inoculate the bacterial solution into aquaculture water; (3) Incubate for 24–48 h at pH 6.0–9.0, temperature 15–30 ℃, and 0.5 mg / L < initial phosphorus concentration ≤ 4.0 mg / L.

[0016] The phosphorus removal method is an in-situ treatment method for aquaculture water bodies.

[0017] The phosphorus removal method, wherein the in-situ treatment involves the formation of visible polyphosphate particles within *Polyphosate Atlanta bacillus* 1-29 cells.

[0018] The aforementioned *Polyphosate Atlantaobacter 1-29* can form visible polyphosphate particles within its cells and exhibits a significant phosphorus removal capacity for water bodies with low phosphorus concentrations.

[0019] The *Polyphosate Atlantaobacter* 1-29 strain maintains stable phosphorus removal capacity within a pH range of 6.0–9.0 and a temperature range of 15–30 °C.

[0020] The aforementioned *Polyphosate Atlantaobacter* 1-29 strain is suitable for use in low-concentration phosphorus-containing water bodies with an initial phosphorus concentration of 0.5–4.0 mg / L.

[0021] The *Polyphosate Atlanta bacillus* 1-29 strain does not produce hemolysis on blood agar plates.

[0022] The *Polyphosate Atlantaobacter 1-29* strain described herein does not exhibit multidrug resistance to commonly used antibiotics in aquaculture.

[0023] Further preferred embodiments of *Polyphosate Atlantaella* of the present invention will be described with the following advantages and features: I. Polyphosphate-accumulating bacteria The present invention provides Polyphosphatidylcholine Atlanta 1-29 ( Atlantibacter sp. Polyphosphate-aggregate 1-29), with accession number CGMCC No. 37506, was deposited on January 22, 2026, at the China General Microbiological Culture Collection Center (CGMCC), address: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The described *Polyphosphate-aggregate Atlantab* 1-29 ( Atlantibacter sp. 1-29), the 16S rDNA gene sequence is shown in SEQ ID No. 1.

[0024] II. Polyphosphate Capacity and Structural Evidence The *Polyphosate Atlantaobacter 1-29* of this invention has a significant intracellular polyphosphate accumulation capacity, which can form polyphosphate particles within the cell, thereby achieving the bioabsorption and enrichment of dissolved phosphorus in water.

[0025] To verify the polyphosphate-accumulating ability of *Polyphosphate-Atlantella* 1-29, metachromatic granule staining was performed on the strain. Specifically, after culturing *Polyphosphate-Atlantella* 1-29 to the logarithmic growth phase under phosphorus-containing conditions, the bacterial cells were stained using the metachromatic granule staining method and observed under an optical microscope. The results showed that obvious metachromatic granules could be observed inside the bacterial cells of the strain. These metachromatic granules were distributed in dark color within the cells, indicating that the strain can form polyphosphate granules within the cells.

[0026] The above results indicate that the *Agniella polyphosphate* 1-29 described in this invention possesses typical physiological characteristics of polyphosphate bacteria. It removes phosphorus from water by accumulating polyphosphates within its cells, providing clear structural and functional experimental evidence for its application in phosphorus removal in phosphorus-containing aquaculture waters.

[0027] III. Environmental adaptability and phosphorus removal performance The *Polyphosate Atlantaobacterium* 1-29 described in this invention exhibits good adaptability to common environmental conditions in aquaculture water bodies, specifically as follows: It can maintain stable growth and high phosphorus removal capacity within a pH range of 6.0–9.0; It still exhibits high physiological activity within a temperature range of 15–30 ℃; It can achieve efficient phosphorus removal in water bodies with low phosphorus concentrations of 0.5–4.0 mg / L.

[0028] Under the above conditions, when the polyphosphate-ladenella Atlanta 1-29 is inoculated into phosphorus-containing water and cultured for 24–48 hours, the phosphorus removal rate in the water can reach over 85%.

[0029] In the method described, phosphorus refers to soluble inorganic phosphorus (orthophosphate).

[0030] IV. Biosafety The *Polyphosate Atlantaobacter 1-29* described in this invention exhibits good biosafety, specifically as follows: No hemolysis occurs when cultured on blood agar plates; It does not exhibit multidrug resistance to commonly used antibiotics in aquaculture.

[0031] Infections, both internal and external, do not cause death in grass carp.

[0032] Therefore, when this strain is used for phosphorus removal in aquaculture water, it is unlikely to have adverse effects on the normal growth and survival of aquatic animals, and it has a safe basis for in-situ application.

[0033] V. Phosphorus Removal Methods in Aquaculture Water The present invention also provides a method for treating phosphorus-containing water bodies in aquaculture using the above-mentioned Polyphosphate Atlantab 1-29, comprising the following steps: Preparation of bacterial suspension: Inoculate *Polyphosate Atlanta bacillus* 1-29 into the culture medium and culture until the logarithmic growth phase to prepare the bacterial suspension; Addition of bacterial solution: Add the bacterial solution to phosphorus-containing water bodies in aquaculture at a certain ratio; Phosphorus removal treatment: Under natural or micro-aeration conditions, at pH 6.0–9.0, temperature 15–30 ℃, and initial phosphorus concentration not exceeding 4.0 mg / L, the treatment lasts for 24–48 h to achieve in-situ removal of phosphorus from the water.

[0034] VI. Application Forms and Applicable Objects Preferably, the polyphosphate-ladenum Atlanta 1-29 is added to the water body in the form of a liquid or solid inoculant. More preferably, the aquaculture water body includes, but is not limited to, pond aquaculture systems, factory-style recirculating aquaculture systems, and cage aquaculture systems.

[0035] Without affecting the normal growth and survival of aquatic animals, this invention can achieve stable removal of phosphorus from aquaculture water.

[0036] Beneficial effects: Compared with the prior art, the present invention provides a polyphosphorus Atlanta bacillus 1-29 with clear polyphosphorus structural characteristics, strong environmental adaptability and high biosafety, which realizes efficient in-situ purification of low-concentration phosphorus-containing water in aquaculture and has good application prospects. (1) Highly efficient phosphorus removal capacity. The polyphosphorus Atlanta bacillus 1-29 described in the present invention can efficiently remove phosphorus in low-concentration phosphorus-containing water. Polyphosphate particles can be formed in its cells, which can enrich the dissolved phosphorus in the water and realize in-situ purification of aquaculture tailwater. Under suitable conditions (pH 6.0–9.0, temperature 15–30℃, initial phosphorus concentration ≤4.0 mg / L), the phosphorus removal rate of water can reach more than 85% within 24–48 hours, which greatly improves the efficiency of tailwater treatment. (2) Good environmental adaptability. The strain can grow stably and maintain its phosphorus removal capacity within a pH range of 6.0–9.0, a temperature range of 15–30 ℃, and an initial phosphorus concentration of ≤4.0 mg / L. It can adapt to various aquaculture environments such as aquaculture ponds, recirculating aquaculture systems, and net cage aquaculture systems, enabling its wide application. (3) High safety. The *Polyphosate Atlanta bacillus* 1-29 described in this invention does not produce hemolysis on blood agar plates and does not exhibit multidrug resistance to commonly used antibiotics in aquaculture. 7Within the safe concentration range of Cfu / mL, it has no toxic effect on grass carp, ensuring that its application in water bodies will not have an adverse impact on aquatic animals and aquatic ecology. (4) Simple operation and can be applied in situ. The strain can be prepared as a liquid or solid bacterial agent and directly added to the aquaculture water without changing the structure of the aquaculture system. It is easy to operate and suitable for large-scale aquaculture wastewater treatment. (5) Combination of structure and function to support the claims. The polyphosphate particles formed by the strain provide clear structural support for the polyphosphate function. Combined with the phosphorus removal effect, it provides sufficient experimental basis and technical support for the polyphosphate characteristics and phosphorus removal methods proposed in the claims of this invention, enhancing the feasibility and protection of the patent. (6) Green and environmentally friendly, sustainable application. This invention removes phosphorus from water by utilizing the biological metabolic capacity of microorganisms, without relying on chemical agents or high-energy-consuming treatment methods, achieving green, low-cost, and sustainable aquaculture wastewater purification, with significant ecological and economic benefits. In summary, the polyphosphate-containing bacillus Atlanta 1-29 and its application method described in this invention have multiple beneficial effects, such as high efficiency in phosphorus removal, strong environmental adaptability, safety and reliability, simple operation, and green environmental protection. It can effectively solve the problem of low-concentration phosphorus treatment in aquaculture wastewater and has significant practical value and promotion prospects. Attached Figure Description

[0037] Figure 1 For the present invention, Polyphosphatidylcholine Atlanta 1-29 ( Atlantibacter sp. 1-29 Colony characteristics diagram;

[0038] Figure 2 For the present invention, Polyphosphatidylcholine Atlanta 1-29 ( Atlantibacter sp. 1-29 Image of the staining results of metachromatic granules;

[0039] Figure 3 For the present invention, Polyphosphatidylcholine Atlanta 1-29 ( Atlantibacter sp. 1-29 Phylogenetic tree diagram;

[0040] Figure 4 For the present invention, Polyphosphatidylcholine Atlanta 1-29 ( Atlantibacter sp. 1-29 Schematic diagram of phosphorus removal effect at different pH levels;

[0041] Figure 5 For the present invention, Polyphosphatidylcholine Atlanta 1-29 ( Atlantibacter sp. 1-29 Schematic diagram of phosphorus removal effect at different temperatures;

[0042] Figure 6 For the present invention, Polyphosphatidylcholine Atlanta 1-29 ( Atlantibacter sp. 1-29 Schematic diagram of phosphorus removal effect at different initial phosphorus concentrations;

[0043] Figure 7 For the present invention, Polyphosphatidylcholine Atlanta 1-29 (Atlantibacter sp. 1-29 (Image of hemolytic test results) Detailed Implementation

[0044] The technical solution of the present invention will be further explained and described below with reference to specific embodiments and accompanying drawings.

[0045] Example 1 Primary screening and purification of polyphosphate-accumulating bacteria

[0046] Objective: To screen candidate strains with potential polyphosphate accumulation (PPI) function from natural sediment samples, purify single colonies, and confirm intracellular PPI particles through blue-white primary screening and metachromatic particle secondary screening, providing a basis for subsequent screening of highly efficient PPI-accumulating bacteria.

[0047] Operating steps:

[0048] In a clean bench, take approximately 5 g of sediment sample and place it in 45 mL of sterilized beef extract peptone liquid medium (formulation: 5 g / L beef extract, 10 g / L peptone, 5 g / L NaCl, pH 7.0). Add a few glass beads to facilitate stirring. Place the culture flask in a shaker at 30 ℃ and 120 r / min for 5 days to promote the growth and enrichment of microorganisms in the sediment.

[0049] The enriched culture medium was serially diluted, with a dilution factor of 10. -1 Up to 10 -6 Take 0.1 mL of each dilution and spread it evenly on YG solid medium plates (formulation: yeast extract 1 g / L, glucose 1 g / L, K2HPO4 0.3 g / L, MgSO4 0.2 g / L, KH2PO4 0.25 g / L, pH 7.2–7.4). Each dilution should be replicated in triplicate. Incubate the plates upside down for 2–3 days and observe for single colonies. Among them, *Actinobacillus polyphosphate* 1-29 (… Atlantibacter sp. 1-29 See the colony characteristic diagram for ). Figure 1 .

[0050] Single colonies with diverse morphologies were selected and purified on YG plates using the streak plating method, repeated at least three times to obtain stable single strains. The purified strains were transferred to beef extract slant agar and incubated at 30 °C for 2 days, then stored at 4 °C for short periods; if necessary, they were preserved with glycerol at -80 °C to ensure long-term viability. The morphological characteristics and storage number of each colony were recorded to provide basic information for subsequent screening.

[0051] The purified strain was inoculated onto YG plates containing 50 mg / L sodium 5-bromo-4-chloro-3-indole phosphate (BCIP) and incubated at 30 °C for 1–2 days. The colony color change was observed; strains that turned blue were identified as candidate bacteria that may have polyphosphate-accumulating function and were further screened for metachromatic particles.

[0052] The isolated and purified strains were rescreened using the metachromatic granule staining method. The metachromatic granule staining kit (Beijing Solarbio Science & Technology Co., Ltd., catalog number G3080) was used for staining. Slides were prepared according to standard procedures: stained with reagent A for 3 min, reagent A was discarded, and the slides were washed with water. Then, stained with reagent B for 1 min, washed with water, blotted dry, and prepared for oil immersion microscopy. The results are as follows: Figure 2 As shown, the metachromatic granules of polyphosphate bacteria appear blue-black, while the rest of the bacterial cell appears green.

[0053] Twenty-one strains that formed polyphosphate particles were screened out and selected as candidate strains for subsequent phosphorus removal efficiency determination.

[0054] Note: This embodiment provides a complete process for screening candidate polyphosphate-accumulating bacteria from natural samples, including enrichment, dilution, plate culture, single colony purification, blue-white screening, and metachromatic particle screening. The recorded strain morphological characteristics and preservation numbers provide a reproducible basis for subsequent experiments, while also supporting the characteristics of "polyphosphate-accumulating bacteria" and "intracellular formation of polyphosphate particles" in the claims.

[0055] Example 2 Phosphorus removal efficiency detection of candidate polyphosphate-accumulating bacteria

[0056] Objective: To screen for highly efficient phosphorus removal strains, providing experimental basis for subsequent optimization of culture conditions and determination of the final patent-protected strain.

[0057] Operating steps:

[0058] Twenty-one candidate polyphosphate-accumulating bacteria strains selected in Example 1 were inoculated into liquid polyphosphate medium (0.5 g sodium acetate, 0.22 g beef extract, 0.2 g (NH4)2SO4, 0.4 g MgSO4·7H2O, 0.002 g FeSO4·7H2O, 1000 ml distilled water, with different phosphorus concentrations added), and cultured at an inoculum size of 1%, ensuring consistent culture conditions for all strains. The cultures were incubated at 30°C and 200 r / min on a shaker for 24 h and 48 h. After incubation, the bacterial suspension was centrifuged at 8000 r / min and 4°C for 5 minutes, and the supernatant was used to determine phosphate (PO42-). 3- Phosphorus content was measured. The phosphorus removal rate was calculated, and parallel replicate experiments were performed to ensure data reliability. Based on the measured phosphorus removal efficiency, the top 6 highly efficient polyphosphate-accumulating bacteria were selected as candidate bacteria.

[0059] Note: This embodiment provides a laboratory method for determining the phosphorus removal efficiency of candidate bacteria, providing data support for the final screening of highly efficient polyphosphate-accumulating bacteria, and also supporting the characteristics of "highly efficient phosphorus-removing strains" in the claims.

[0060] Example 3 Phosphorus removal efficiency detection and stability analysis under different conditions

[0061] Objective: To test the phosphorus removal capacity of the top 6 high-efficiency candidate polyphosphate-accumulating bacteria under different culture conditions, clarify the performance of each strain under different pH, temperature and initial phosphorus concentration conditions, and determine the stable and efficient phosphorus removal condition range, so as to provide experimental basis for final strain screening and subsequent application.

[0062] Operating steps:

[0063] 1. Strains preparation: The top 6 high-efficiency candidate strains screened in Example 2 were inoculated into liquid polyphosphate medium and cultured to the logarithmic growth phase to ensure consistent bacterial concentration (OD). 600 ≈1), used as experimental bacterial solution.

[0064] 2. Determination of phosphorus removal efficiency under single-factor conditions: pH conditions: The cells were cultured at pH 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, and 9.0, respectively, and the phosphorus removal rate was measured after 24 h and 48 h.

[0065] Temperature conditions: The cells were cultured at 15 ℃, 20 ℃, 25 ℃, 30 ℃ and 35 ℃ respectively, and the phosphorus removal rate was measured after 24 h and 48 h.

[0066] Initial phosphorus concentration conditions: The cells were cultured in water with concentrations of 0.5 mg / L, 1.0 mg / L, 2.0 mg / L, 3.0 mg / L, and 4.0 mg / L, and the phosphorus removal rate was measured after 24 h and 48 h.

[0067] 3. Data Recording and Analysis Record the phosphorus removal efficiency of each strain under different conditions.

[0068] The effects of different conditions on phosphorus removal rate were analyzed, and the stability of each strain under different environments was evaluated.

[0069] Determine the stable condition range for each strain, that is, within this range, it can maintain a high phosphorus removal efficiency.

[0070] Comprehensive analysis and screening identified strains that are stable and efficient at phosphorus removal under various conditions, providing a scientific basis for the final screening of highly efficient polyphosphate-accumulating bacteria.

[0071] Results: Statistical results of phosphorus removal efficiency of polyphosphate-accumulating bacteria 1-29 under different conditions are shown below. Figures 4-6 As shown in the figure. The horizontal axis represents different pH values, temperatures, or initial phosphorus concentrations, while the vertical axis represents the percentage of phosphorus removal efficiency.

[0072] Note: This embodiment, through the determination of phosphorus removal efficiency and stability analysis under single-factor conditions, not only provides data support for the subsequent final strain screening, but also clarifies the stable phosphorus removal condition range of each candidate bacteria, supporting the characteristics of "stable and efficient phosphorus removal strains" in the claims, and providing an important reference for the in-situ application of polyphosphate-accumulating bacteria in aquaculture water bodies.

[0073] Example 4 Final screening and safety verification of highly efficient polyphosphate-accumulating bacteria Objective: To screen out the strains to be protected under patents by considering the phosphorus removal efficiency, stability and safety of each strain, and to describe their characteristics and application prospects.

[0074] Operation plan and results: 1. Final screening: The phosphorus removal rates of the six highly efficient bacteria under the optimized conditions in Example 3 were measured under different pH, temperature and initial phosphorus conditions. Their stability, efficiency and safety were compared, and the final preferred strains were determined by comprehensive analysis.

[0075] 2. Final strain identification Based on phosphorus removal efficiency, culture stability, and safety, *Polyphosate Atlantaella* 1-29 was determined to be... Atlantibacter sp . 1-29 (CGMCC No. 37506) is the highly efficient polyphosphate-accumulating strain that is ultimately protected by this invention.

[0076] 3. Security Verification Hemolysis test: After culturing on blood agar plates, no hemolytic ring should form after 24 hours. Figure 7 As shown.

[0077] Antibiotic susceptibility test: Commonly used aquaculture antibiotics (such as chloramphenicol, tetracycline, penicillin, and lincomycin) were tested, and the results showed no multidrug resistance.

[0078] In vivo / in vitro infection experiments: The infection solution was added to the aquaculture water in a specific ratio, maintaining a bacterial concentration of approximately 1×10⁻⁶ in the water. 5 Cfu / mL, 1×10 6 Cfu / mL and 1×10 7 Cfu / mL), with an equal volume of sterile water added to the blank control group; grass carp injection infection: grass carp were intraperitoneally injected into a low-dose injection group (bacterial concentration approximately 1×10⁻⁶ Cfu / mL); grass carp were divided into a low-dose injection group (bacterial concentration approximately 1×10⁻⁶ Cfu / mL) and a blank control group were added with an equal volume of sterile water; grass carp were injected with infection: 5 Cfu / mL) and high-dose injection group (bacterial concentration approximately 1×10⁻⁶ ... 7The control group was injected with 0.6% sterile saline. The injection dose was 0.2 mL. Each group had 3 replicates, with 10 fish per tank. During the experiment, the fish were fed normally, and the behavior, body surface, and survival rate of grass carp in each tank were recorded and observed at regular intervals every day for 7 days. The results showed that the grass carp in each group had no abnormalities, and the survival rate was 100%. In the in vitro and in vivo infection test of grass carp, the bacterium did not show any pathogenicity.

[0079] Strains and their application demonstrations Strain characteristics: The cells exhibit prominent polyphosphate granules and stable morphology. The 16S rDNA sequence of strain 1-29 is 1442 bp in length, and its gene sequence is shown in SEQ ID No. 1. The strain sequence was uploaded to the NCBI database and compared with existing bacterial 16S rDNA gene sequences. The results indicate that this bacterium is... Atlantibacter sp. A phylogenetic tree was constructed using the neighbor-joining method in MEGA software to analyze the genetic characteristics of the strains, determine their species status and evolutionary position, and the results are shown in [Figure number missing]. Figure 3 .

[0080] Phosphorus removal capacity: Under conditions of low phosphorus concentration (0.5–4.0 mg / L), pH 6.0–9.0, and temperature 15–30 ℃, after cultivation for 24–48 h, a phosphorus removal rate of ≥85% can be achieved in water.

[0081] Application: It can be prepared as a liquid or solid bacterial agent and used directly for in-situ phosphorus removal in aquaculture water bodies without changing the structure of the aquaculture system.

[0082] Note: This embodiment fully supports the characteristics of the "final high-efficiency polyphosphate strain" in the claims, and demonstrates the safety and application potential of the strain, providing sufficient basis for patent protection.

Claims

1. A polyphosphorus Atlanta bacillus 1-29, characterized in that: It was identified as Atlanta bacillus ( Atlantibacter sp. It was deposited on January 22, 2026 at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 37506.

2. The *Polyphosate Atlantaobacter 1-29* according to claim 1, characterized in that: Its 16S rDNA gene sequence is shown in SEQ ID No.

1.

3. The application of Polyphosphatobacterium Atlanta 1-29 as described in claim 1 in phosphorus removal in aquaculture water.

4. The application according to claim 3, characterized in that: The aquaculture water bodies include pond aquaculture systems, factory-style recirculating aquaculture systems, or cage aquaculture systems.

5. The application according to claim 3, characterized in that: The aquaculture water has a pH of 6.0–9.0, a temperature of 15–30 ℃, and an initial phosphorus concentration of 0.5–4.0 mg / L.

6. A bacterial agent or bacterial solution, characterized in that: It includes Polyphosphatobacterium Atlanta 1-29 as described in claim 1.

7. The application of the bacterial agent or bacterial solution as described in claim 6 in phosphorus removal in aquaculture water.

8. A method for removing phosphorus from phosphorus-containing water in aquaculture using *Acetobacter lysophosphatidylcholine* 1-29 as described in any one of claims 1-2, characterized in that: Includes the following steps: (1) The *Polyphosate Atlanta bacillus* 1-29 was cultured to the logarithmic growth phase to prepare a bacterial culture; (2) Inoculate the bacterial solution into aquaculture water; (3) Incubate for 24–48 h at pH 6.0–9.0, temperature 15–30 ℃, and initial phosphorus concentration ≤ 0.5 mg / L ≤ 4.0 mg / L.

9. The phosphorus removal method according to claim 8, characterized in that: The phosphorus removal method is an in-situ treatment method for aquaculture water bodies.

10. The phosphorus removal method according to claim 9, characterized in that: The in-situ treatment method involves the formation of visible polyphosphate particles within *Polyphosate Atlanta bacillus* 1-29 cells.

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