A fatty acid photodecarboxylase mutant, and a preparation method and application thereof

Abstract

This application discloses a fatty acid photodecarboxylase mutant, its preparation method, and its applications, relating to the field of protein engineering technology. The amino acid sequence of the fatty acid photodecarboxylase mutant of this application comprises a sequence obtained by mutating the 624th amino acid residue site in the sequence shown in SEQ ID NO.1, wherein the mutation type at the 624th amino acid residue site is selected from any one of A624C, A624N, A624Q, and A624S. Compared with the wild type, the preferred mutant of this application exhibits 2-fold and 1.3-fold increased enzyme activity and blue light tolerance, respectively. It achieves a conversion rate of over 90% for the long-chain fatty acid palmitic acid within 8 hours at 30°C, significantly enhancing the enzyme's stability and catalytic efficiency under blue light irradiation. This helps overcome the bottleneck of photoinactivation caused by blue light in the industrial application of fatty acid photodecarboxylase, and has excellent application prospects in biofuel synthesis and green chemistry.

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