Oligonucleotide-based strategies for capture, detection, adaptation and sequencing of TRNA using nanopore technology

By using a sequencing library preparation method, 3′ and 5′ splice oligonucleotides are hybridized with tRNA, combined with DNA ligase and nanopore direct sequencing, which solves the problem of difficulty in quantifying and detecting modifications in existing tRNA sequencing technologies, and achieves accurate quantification and detection of tRNA abundance and modifications.

CN122180787APending Publication Date: 2026-06-09NORTHEASTERN UNIV (US) +1
View PDF 2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NORTHEASTERN UNIV (US)
Filing Date
2024-10-31
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing tRNA sequencing methods are difficult to accurately quantify and monitor its modification dynamics, and cannot simultaneously detect and quantify tRNA abundance and modification.

Method used

The sequencing library preparation method was used, 3′ and 5′ splice oligonucleotides were hybridized with tRNA, and the product was formed by DNA ligase. Reverse transcription and direct nanopore sequencing were performed, and ion current analysis was performed using longer adaptor DNA oligonucleotides.

Benefits of technology

It enables direct sequencing and modification detection of tRNA, improves quantification capabilities, and allows for the simultaneous detection of tRNA abundance and modification, thereby enhancing the accuracy of ion current analysis.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure FT_1
    Figure FT_1
  • Figure FT_2
    Figure FT_2
  • Figure FT_3
    Figure FT_3
Patent Text Reader

Abstract

Disclosed are methods of preparing sequencing libraries to quantify tRNA abundance and tRNA modifications in an RNA sample, the methods comprising contacting the RNA with an oligonucleotide in the presence of a ligation agent, and performing nanopore direct sequencing. Also disclosed are kits for performing the methods.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • Characterization of individual polymer molecules based on monomer-interface interactions

    US6015714A

  • Characterization of individual polymer molecules based on monomer-interface interactions

    US6362002B1