A fhl3 gene promoter snp molecular marker related to pig meat production traits and application thereof

By screening for SNP sites in the promoter region of the FHL3 gene on pig chromosome 6, the problem of identifying genetic markers in pig breeding was solved, which improved the efficiency of pig breeding and the improvement of meat quality traits, and achieved more precise genetic selection.

CN118995938BActive Publication Date: 2026-06-09HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Filing Date
2024-06-27
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing technologies struggle to efficiently identify and screen genetic markers associated with meat production traits in pigs, resulting in a complex and inefficient pig breeding process.

Method used

By cloning the SNP site in the promoter region of the FHL3 gene at 93990293 bases on pig chromosome 6, primer pairs were designed for PCR amplification and sequencing. Genetic markers associated with pig meat production traits were screened out, and the association between genotype and phenotype was analyzed using SAS statistical software.

Benefits of technology

It significantly improved the efficiency of pig breeding. Individuals with the CC genotype outperformed those with the TT genotype in terms of birth weight, eye muscle area, and traits at 100kg age. It also had the advantage of reducing backfat thickness in live animals, providing important guidance for genetic selection and breeding.

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Abstract

This invention belongs to the fields of molecular biology and molecular marker technology, and relates to a SNP molecular marker for the FHL3 gene promoter associated with meat production traits in pigs and its application. The genetic marker is cloned from locus 93990293 on pig chromosome 6. Whole-genome DNA was extracted from pig blood, and primers were designed based on the pig genome sequence (NC_010448.4) published in the NCBI database. A C or T base substitution exists at position 585 of the primer amplification sequence, leading to polymorphism at this position. Genotyping of the SNP locus using direct sequencing technology and association analysis showed that, compared to individuals with other genes, individuals with the CC genotype at position 585 had higher birth weight, larger eye muscle area, shorter time to reach 100 kg, and less backfat thickness. This invention provides a new marker for marker-assisted selection in pigs, and its application can accelerate the process of improving high-quality pig breeding.
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