Multi-parameter integrated high-efficiency synergistic germination device

Abstract

This invention discloses a highly efficient, multi-parameter integrated synergistic germination device, comprising a parameter environment chamber and a central column. Multiple parameter environment chambers are arranged in a ring to form an operating position. Each parameter environment chamber can serve as a parameter path for sample dishes to enter and germinate. Two or more parameter environment chambers can synergistically serve as a parameter path for sample dishes to germinate according to a preset entry sequence. The central column is located within the operating position, and multiple telescopic support rods are arranged on its outer wall. Each telescopic support rod has a sample dish attached to its distal end. By integrating multiple sample dishes onto a central column and supporting them with telescopic support rods, this invention allows each sample dish to correspond to a specific parameter path, resulting in a single experimental outcome. This significantly enhances the integration of sample dish layout and experimental path management, making operation simpler and management less complex when sample dishes participate in the difficult-to-manage multi-parameter synergistic germination process.

Description

Technical Field

[0001] This invention relates to the field of germination experimental technology, specifically to a multi-parameter integrated high-efficiency synergistic germination device. Background Technology

[0002] In the fields of seed physiology, agricultural breeding, and forestry research, multi-factor, multi-level comparative experiments are typically required to investigate the synergistic effects of multiple parameters such as temperature, humidity, and light on seed germination. The core of such experiments lies in placing the same batch of seed samples in environments with different parameter combinations for cultivation, and continuously tracking and recording their germination process and status.

[0003] In existing technologies, the conventional approach to conducting such multi-parameter comparative experiments is to configure a separate culture container (such as a petri dish) for each independent combination of parameters (i.e., a "parameter path"), and to label the containers manually to distinguish their corresponding experimental parameters and culture stages. Researchers need to manually manage these discrete containers, recording their location, current environment, and observation data.

[0004] However, when there are many parameter paths, this method requires each sample dish to be individually labeled and relevant information to be recorded one by one, which is cumbersome to manage and leads to a greater risk of sample confusion and record errors. This makes the tracking and management of the existing operation method highly discrete and less integrated, especially in germination experiments with some parameters working together, where the tracking and management is even more difficult. Summary of the Invention

[0005] The purpose of this invention is to provide a highly efficient and collaborative germination device with integrated multi-parameter parameters, so as to solve the technical problems of high management difficulty and high risk of misoperation caused by the discrete management of germination experiments with variable parameters in the prior art.

[0006] To solve the above-mentioned technical problems, the present invention specifically provides the following technical solution: A multi-parameter integrated high-efficiency synergistic germination device, characterized in that it comprises: A parameter environment box, wherein multiple parameter environment boxes are arranged in a ring to form an operating position for placing sample dishes; A central column is located within the operating position. Multiple telescopic support rods are provided on the outer wall of the central column. The multiple telescopic support rods are arranged linearly and vertically along the axial direction of the central column. The telescopic support rods are all telescopically extended and retracted towards the parameter environment chamber along the radial direction of the central column. A sample dish is provided at the distal end of each telescopic support rod. The proximal end of the telescopic support rod is rotatably mounted on the outer wall of the central column around the axis of the central column. The telescopic support rod rotates around the axis of the central base column to drive the sample dish to circulate in different parameter environment chambers; Each of the parameter environment boxes can serve as a parameter path for the sample dish to enter the germination process; Two or more of the aforementioned parameter environment boxes, used in a preset entry sequence, can work together as a parameter path to promote germination in the sample dish.

[0007] As a preferred embodiment of the present invention, the parameter environment chamber includes at least a sterilization chamber, a low-temperature lamination chamber, a constant-temperature incubator, and a variable-temperature incubator. The sterilization chamber, the low-temperature lamination chamber, the constant-temperature incubator, and the variable-temperature incubator are combined to form eight effective parameter paths, which are as follows: Germination was initiated separately using a constant temperature incubator; Separate germination in a variable temperature incubator; They are sequentially placed in a low-temperature stratification chamber and a constant-temperature incubation chamber for synergistic germination. They are sequentially placed into a low-temperature stratification box and a variable-temperature incubator for synergistic germination. The germination process involves sequentially entering a sterilization chamber and a constant temperature incubator for synergistic germination. The cells are sequentially placed in a sterilization chamber and a variable-temperature incubator for synergistic germination. The cells are sequentially placed in a sterilization chamber, a low-temperature stratification chamber, and a constant-temperature incubator for synergistic germination. The cells are sequentially placed in a sterilization chamber, a low-temperature stratification chamber, and a variable-temperature incubator for synergistic germination. The telescopic support rod is provided with eight rods, and each telescopic support rod participates in a separate parameter path.

[0008] As a preferred embodiment of the present invention, the doors of the sterilization box, the low temperature layering box, the constant temperature incubator and the variable temperature incubator are all provided with opening structures, and the telescopic support rod sends the sample dish into the parameter environment box through the opening structure; The opening structure includes a through opening that passes through the box door and a closing door provided on the box door. The closing door is slidably disposed on both sides of the through opening, and the closing door closes the through opening by sliding relative to each other from both sides of the telescopic support rod.

[0009] As a preferred embodiment of the present invention, the disinfection box and the low-temperature lamination box are each provided with four disinfectant boxes and four lamination sand boxes, and each of the four disinfectant boxes and the four lamination sand boxes corresponds to one sample dish.

[0010] As a preferred embodiment of the present invention, a dish box is provided at the distal end of the telescopic support rod, and the sample dish is movably disposed within the dish box; The sample dish includes a base dish and a lid. Both the base dish and the lid are provided with dense flow holes. The base dish is detachably disposed in the dish box for loading the sample. The lid is movably closed on the base dish. The dish box is provided with a limiting member to prevent the lid from detaching from the base dish.

[0011] As a preferred embodiment of the present invention, a plurality of partition plates are fixedly disposed on the bottom surface of the substrate. The partition plates extend downward and protrude from the outer bottom surface of the substrate. The plurality of partition plates divide the bottom surface of the substrate into a plurality of culture media for placing samples. The culture media are used to be stored in the constant temperature incubator or the variable temperature incubator for cultivation and germination.

[0012] As a preferred embodiment of the present invention, the telescopic support rod includes a telescopic rod and a fixed rod. One end of the telescopic rod is connected to a container, and the other end is slidably inserted into the fixed rod. One end of the fixed rod is connected to the telescopic rod, and the other end is connected to the central base column. A servo motor is axially slidably installed inside the fixed rod, and the output end of the servo motor is connected to the telescopic support rod to drive the telescopic support rod to rotate axially.

[0013] As a preferred embodiment of the present invention, the telescopic rod includes a straight rod body, one end of which is connected to the fixed rod, and the other end is fixedly connected to a bent rod head, which extends downward to connect to the container. The disinfectant box is slidably mounted longitudinally inside the disinfection chamber.

[0014] Compared with the prior art, the present invention has the following advantages: This invention integrates multiple sample dishes into a central base column and is supported by a telescopic support rod, which improves the integration of sample dish layout and experimental path management, avoiding sample confusion and recording errors that are easily caused by traditional multi-container manual labeling management.

[0015] Furthermore, the present invention enables the sample dish to flow orderly between multiple parameter environmental chambers according to a preset path through the rotational connection of the telescopic support rod on the central base column, thereby simplifying the complex and tedious manual handling and multi-stage collaborative operation. This makes the multi-parameter collaborative germination process not only easier to operate, but also less complex to manage. Attached Figure Description

[0016] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely exemplary, and those skilled in the art can derive other embodiments based on the provided drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the opening structure of the present invention; Figure 3 This is an exploded view of the sample box of the present invention; Figure 4 This is a schematic diagram of the structure of the culture dish of the present invention; Figure 5 This is a schematic diagram of the servo motor of the present invention.

[0018] The labels in the diagram represent the following: 1. Parameter environment chamber; 2. Operating position; 3. Sample dish; 4. Central base column; 5. Telescopic support rod; 6. Sterilization chamber; 7. Low temperature lamination chamber; 8. Constant temperature incubator; 9. Variable temperature incubator; 10. Opening structure; 11. Through opening; 12. Closable door; 13. Sterilizer box; 14. Lamination sand box; 15. Dish box; 16. Bottom dish; 17. Dish lid; 18. Divider plate; 19. Culture dish; 20. Telescopic rod; 21. Fixed rod; 22. Servo motor; 23. Straight rod body; 24. Bent rod head. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] like Figures 1 to 4 As shown, the present invention provides a multi-parameter integrated high-efficiency synergistic germination device, comprising: The parameter environment chamber 1 is a basic component for realizing multi-parameter germination. Multiple parameter environment chambers 1 are set up in a ring-shaped and uniform arrangement. The doors of each parameter environment chamber 1 are all set facing the center of the enclosure. The multiple parameter environment chambers 1 enclose a ring-shaped space. The center of this ring-shaped space is the operation position 2 for placing the sample dish 3.

[0021] Each parameter environment chamber 1 is equipped with an independent parameter control system, capable of maintaining its own specific germination environment parameters. This allows it to serve as an independent parameter path for sample dishes 3 to enter and perform single-parameter germination. Simultaneously, because multiple parameter environment chambers 1 are arranged in a ring and their parameters can be independently controlled, any two or more parameter environment chambers 1 can form a continuous multi-parameter collaborative germination path according to a preset entry sequence. Sample dishes 3 sequentially enter each parameter environment chamber 1 within this path to complete multi-parameter collaborative germination. The preset entry sequence can be set according to the germination requirements of different samples.

[0022] A central column 4 is set in the middle of the operation position 2, i.e., the multiple parameter environment chambers 1. The central column 4 is a supporting component, and its axis is arranged in a vertical direction. On the outer wall of the central column 4, multiple telescopic support rods 5 are linearly and uniformly arranged along its axis (i.e., the vertical direction). The arrangement direction of all telescopic support rods 5 is consistent with the radial direction of the central column 4. That is, each telescopic support rod 5 extends and retracts along the radial direction of the central column 4 towards the annular parameter environment chambers 1, so that its length is adjustable, thereby driving the sample dish 3 on its distal end to enter and exit the parameter environment chamber 1.

[0023] Each telescopic support rod 5 has a sample dish 3 fixedly mounted at its distal end (the end furthest from the central base column 4). The sample dish 3 is used to hold the sample to be germinated. The proximal end of the telescopic support rod 5 (the end closest to the central base column 4) is rotatably mounted on the outer wall of the central base column 4 around its axis via a rotating connection structure. This rotating connection structure can be a ring-fit structure, i.e., as shown in the image. Figure 1 The telescopic support rod 5 shown is fixedly mounted on the ring sleeve at its end. The ring sleeve is coaxially rotatably mounted on the central base column 4. The ring sleeve is connected to a locking element that can fix the rotation position of the device, ensuring that the telescopic support rod 5 can rotate flexibly, thereby driving the sample dish 3 at its far end to flow between different parameter environment boxes 1, realizing the switching of the sample dish 3 in different parameter paths.

[0024] Furthermore, this embodiment further defines the specific type, quantity, and combination method of parameter environment boxes 1, as follows: like Figure 1As shown, the parameter environment chamber 1 includes at least four types: a sterilization chamber 6, a low-temperature stratification chamber 7, a constant-temperature incubator 8, and a variable-temperature incubator 9. All four parameter environment chambers 1 are arranged in a uniform ring to form the operating position 2. The structures and functions of the four parameter environment chambers 1 are independent, each used to implement different process steps in the germination process: The sterilization chamber 6 is used to sterilize the sample and remove bacteria from the sample surface; the low-temperature stratification chamber 7 provides a low-temperature stratification environment for the sample and contains stratified sand inside to encapsulate the sample within the chamber at that position, meeting the low-temperature dormancy requirements for germination of some samples; the constant-temperature incubator 8 provides a constant temperature environment for the sample, achieving constant-temperature germination; and the variable-temperature incubator 9 provides a periodically changing temperature environment for the sample, simulating temperature changes in the natural environment to improve germination efficiency and germination rate.

[0025] The above-mentioned parameter environment box 1 can form eight valid parameter paths through different combinations. The eight valid parameter paths are as follows: 1. Germination in constant temperature incubator 8: Sample dish 3 is placed only in constant temperature incubator 8, and the entire germination process is completed in a constant temperature environment without sterilization or low temperature stratification. 2. Germination in variable temperature incubator 9: Sample dish 3 is placed only in variable temperature incubator 9, and the entire germination process is completed in a variable temperature environment without sterilization or low temperature stratification. 3. The sample dish 3 enters the low temperature layering chamber 7 and the constant temperature incubator 8 in sequence for germination: The sample dish 3 first enters the low temperature layering chamber 7. After the low temperature layering process is completed, the sample dish 3 is rotated by the telescopic support rod 5 and transferred to the constant temperature incubator 8. The subsequent germination process is completed in a constant temperature environment. The low temperature layering is performed first, followed by the constant temperature germination operation. 4. Germination is carried out in sequence in the low temperature layering chamber 7 and the variable temperature incubator 9: The sample dish 3 first enters the low temperature layering chamber 7 to complete the low temperature layering treatment, and then is transferred to the variable temperature incubator 9 to complete the subsequent germination process in a variable temperature environment. The low temperature layering is performed first, and then the variable temperature germination operation is performed. 5. Germination is carried out sequentially in the sterilization chamber 6 and the constant temperature incubator 8: Sample dish 3 first enters the sterilization chamber 6 for sterilization, and then is transferred to the constant temperature incubator 8 to complete the subsequent germination process under constant temperature conditions. Sterilization is performed first, followed by constant temperature germination. 6. Germination is carried out sequentially in the sterilization chamber 6 and the variable-temperature incubator 9: Sample dish 3 first enters the sterilization chamber 6 for sterilization, and then is transferred to the variable-temperature incubator 9 to complete the subsequent germination process in a variable-temperature environment. Sterilization is performed first, followed by variable-temperature germination. 7. Germination is initiated sequentially in the sterilization chamber 6, the low-temperature lamination chamber 7, and the constant-temperature incubator 8: Sample dishes 3 first undergo sterilization in the sterilization chamber 6, then are transferred to the low-temperature lamination chamber 7 for low-temperature lamination, and finally transferred to the constant-temperature incubator 8 to complete the subsequent germination process under constant temperature conditions. The sterilization, low-temperature lamination, and constant-temperature germination processes are performed sequentially. 8. Germination is initiated sequentially in the sterilization chamber 6, low-temperature lamination chamber 7, and variable-temperature incubator 9: Sample dishes 3 first undergo sterilization in the sterilization chamber 6, then are transferred to the low-temperature lamination chamber 7 for low-temperature lamination, and finally transferred to the variable-temperature incubator 9 to complete the subsequent germination process under variable-temperature conditions. The sterilization, low-temperature lamination, and variable-temperature germination processes are performed sequentially.

[0026] like Figure 1 As shown, corresponding to the above eight effective parameter paths, there are eight telescopic support rods 5. The eight telescopic support rods 5 are arranged linearly and vertically evenly along the axis of the central base column 4. Each telescopic support rod 5 corresponds to one effective parameter path. That is, the sample dish 3 on each telescopic support rod 5 only performs germination operation according to its corresponding parameter path. The eight telescopic support rods 5 can work simultaneously to achieve simultaneous germination of eight different parameter paths, which greatly improves germination efficiency.

[0027] like Figure 1 As shown, the eight telescopic support rods 5 on the central base column 4 form eight layers. The sample dish 3 on each telescopic support rod 5 corresponds to a parameter path. At their respective positions, an opening is set on the corresponding parameter environment box 1 for the sample dish 3 to enter.

[0028] Specifically, the parameter path is as follows: Factors and level settings: Factor A (Disinfectant Treatment): A1 (No disinfectant added), A2 (1% sodium hypochlorite solution added); Factor B (low-temperature refrigeration): B1 (no low-temperature refrigeration), B2 (4℃ low-temperature wet sand stratification treatment). Factor C (culture temperature): C1 (25℃ constant temperature culture), C2 (15℃ / 25℃ variable temperature culture, 12 hours light / 12 hours dark); Orthogonal experimental table: First, seed selection and counting were carried out: 2400 plump, uniformly sized, and disease-free Korean pine seeds were manually selected. They were randomly divided into 24 groups (8 treatments × 3 replicates), with 100 seeds in each group, and placed into numbered mesh bags.

[0029] Soaking seeds: Soak the selected qualified seeds in tap water for 3 to 4 days, stirring thoroughly, and changing the water once a day during this period.

[0030] Shell breaking treatment: To overcome the seed coat barrier, all seeds are mechanically broken. Using a scalpel, gently break the seed coat at the end opposite the hilum (chalaza end), to a depth that just penetrates the waxy layer and slightly touches the kernel (about 0.3-0.5 mm), taking care not to damage the endosperm and embryo.

[0031] Then, experiments were conducted in groups according to the orthogonal array. Finally, the germination effects of different groups were compared, and the final germination rate, mold rate, average germination time and other data of the 8 groups were statistically analyzed.

[0032] Among them, disinfection treatment: Group A1 (no disinfectant added); Group A2 (with added disinfectant): Disinfect the seeds after they have cracked by soaking them in a 1% sodium hypochlorite solution. Low-temperature refrigeration pretreatment: Group B1 (not refrigerated); Group B2 (4℃ refrigeration): Mix the sterilized and soaked seeds with moist, sterilized river sand (approximately 60% humidity; it should clump together when squeezed but crumble easily when released) at a volume ratio of 1:5. Place the mixture in sample dish 3, ensuring it is permeable to air, and place it in a 4℃ low-temperature stratification chamber 7 for 14 days. Check the humidity every 3-4 days during this period to prevent excessive dryness or moisture, which could lead to mold. After 14 days, remove the seeds, wash off the sand, and prepare for sowing.

[0033] Germination culture in incubator: Sowing: After completing their respective pretreatments, all seeds from each group were sown uniformly. Two layers of moistened qualitative filter paper were placed in clean petri dishes as germination beds. The seeds were evenly arranged on the filter paper, with the hilum facing down, 25 seeds per dish (i.e., 100 seeds per replicate group were divided into 4 petri dishes). The petri dishes were covered with lids and placed in an incubator with a pre-set temperature.

[0034] Cultivation conditions: Group C1 (25℃ constant temperature): The incubator is set to a constant temperature of 25℃, and kept in darkness for 24 hours or in dim light for 12 hours / darkness for 12 hours.

[0035] Group C2 (15℃ / 25℃ variable temperature): The incubator was set to a variable temperature program: 25℃ (12 hours of light) -> 15℃ (12 hours of darkness).

[0036] Therefore, based on the above orthogonal experimental method, the combination of the central column 4 and the sample dish 3 can effectively manage the multi-parameter experimental process.

[0037] Furthermore, the doors of the sterilization chamber 6, the low-temperature stratification chamber 7, the constant temperature incubator 8, and the variable temperature incubator 9 are all equipped with opening structures 10 that are compatible with the telescopic support rod 5. The position and size of the opening structure 10 are matched with the arrangement position and diameter of the telescopic support rod 5 and the sample dish 3 at its front end, ensuring that the telescopic support rod 5 can smoothly extend into the interior of each parameter environment chamber 1 through the opening structure 10, and send the sample dish 3 into the parameter environment chamber 1 for germination. After the sample dish 3 enters, the opening structure 10 closes to prevent leakage or fluctuation of the environmental parameters inside the parameter environment chamber 1.

[0038] like Figure 2 As shown, the opening structure 10 specifically includes two parts: a through opening 11 and a closing door 12. The through opening 11 is a through hole penetrating both the inner and outer sides of the chamber door. Its shape can be rectangular, circular, or other suitable shapes. The size of the through opening 11 is slightly larger than the size of the telescopic support rod 5 and the sample dish 3 to ensure that the telescopic support rod 5 and the sample dish 3 can pass through smoothly. The closing door 12 is installed on the chamber door and located on both sides of the through opening 11. The closing door 12 and the chamber door are connected by a sliding connection structure (such as a slide rail slider cooperation structure), allowing the closing door 12 to slide relative to each other along both sides of the through opening 11. A seal is provided on the inner surface of the closing door 12 to better seal the through opening 11 and maintain the seal within the parameter environment chamber 1.

[0039] When the telescopic support rod 5 carrying the sample dish 3 needs to enter the parameter environment chamber 1, the closing doors 12 on both sides slide along the slide rail away from the center of the through opening 11, opening the through opening 11. After the telescopic support rod 5 extends in, it sends the sample dish 3 to the preset position inside the parameter environment chamber 1. After the sample dish 3 is placed in place, the closing doors 12 on both sides slide along the slide rail towards the center of the through opening 11, closing the through opening 11 by clamping the telescopic support rod 5, isolating the inside of the parameter environment chamber 1 from the outside world, ensuring the stability of the germination environment parameters inside, and avoiding interference from the external environment to the germination process.

[0040] The disinfection chamber 6 is equipped with four disinfectant boxes 13, which are evenly arranged inside the chamber and each of the four disinfectant boxes 13 corresponds to a sample dish 3. The low-temperature lamination chamber 7 is equipped with four lamination sand boxes 14, which are evenly arranged inside the chamber and each of the four lamination sand boxes 14 corresponds to a sample dish 3.

[0041] The four disinfectant boxes 13 inside the disinfection chamber 6 correspond to the four parameter paths that need to be disinfected. Their positions correspond one-to-one with the insertion positions of the sample dishes 3 on the corresponding telescopic support rods 5. When the telescopic support rods 5 insert the sample dishes 3 into the disinfection chamber 6, the sample dishes 3 are exactly above or inside the corresponding disinfectant boxes 13. The disinfectant boxes 13 contain disinfectant, which can accurately disinfect the samples in the sample dishes 3. The four disinfectant boxes 13 can be added to the disinfectant independently to meet the disinfection needs of different samples. Similarly, the four lamination sand boxes 14 inside the low-temperature lamination chamber 7 are also positioned one-to-one with the insertion positions of the sample dishes 3 on the corresponding telescopic support rods 5. The lamination sand boxes 14 contain lamination sand. When the sample dishes 3 are inserted into the low-temperature lamination chamber 7, the operator transfers the lamination sand into the sample dishes 3. After completing this step of the experiment, the lamination sand can also be removed from the chamber.

[0042] Among them, such as Figure 3 As shown, a dish box 15 is fixedly installed at the far end of the telescopic support rod 5. The dish box 15 is a box structure with an open top. The sample dish 3 is movably placed in the dish box 15, which can be freely put in and taken out, making it convenient to load samples, clean and perform other operations on the sample dish 3. At the same time, the dish box 15 can fix and protect the sample dish 3, preventing the sample dish 3 from tipping over or falling during the transfer process.

[0043] The sample dish 3 specifically includes two parts: a bottom dish 16 and a lid 17. The bottom dish 16 is used to hold the sample to be germinated, and the lid 17 is movably closed on the bottom dish 16 to protect the sample and prevent it from being contaminated or losing moisture during the germination process. In order to ensure that the gas and moisture in the germination environment can come into smooth contact with the sample, both the bottom dish 16 and the lid 17 are provided with dense flow holes. The flow holes are evenly distributed on the bottom surface of the bottom dish 16 and the top surface of the lid 17. The size of the holes is adapted to the size of the sample to be germinated, so as to prevent the sample from leaking out of the flow holes and ensure that the environmental parameters can act on the sample efficiently.

[0044] The bottom dish 16 is detachably installed inside the dish box 15, meaning that the bottom dish 16 can be completely removed from the dish box 15 for easy sample loading and cleaning. The dish box 15 is equipped with a limiting component, which can be a structure such as an elastic buckle or a baffle. When the dish lid 17 is placed on the bottom dish 16, the limiting component can limit the dish lid 17, preventing it from falling off the bottom dish 16. This ensures that the dish lid 17 remains closed during the sample dish 3 transfer and germination process, protecting the stability of the sample.

[0045] Furthermore, such as Figure 4As shown, multiple partition plates 18 are fixedly installed on the bottom surface of the bottom plate 16. The partition plates 18 are made of hard and corrosion-resistant material. The height direction of the partition plates 18 extends downward and protrudes beyond the outer bottom surface of the bottom plate 16. That is, the lower end of the partition plates 18 is higher than the outer bottom surface of the bottom plate 16, forming a support structure while dividing the bottom surface of the bottom plate 16.

[0046] Multiple partition plates 18 work together to divide the bottom surface of the base dish 16 into multiple independent culture dishes 19. Each culture dish 19 is of uniform size. Using the aforementioned germination method, pre-treated (sterilized, stratified) seeds are placed in multiple batches within the culture dishes 19. Finally, the culture dishes 19 are placed in a constant temperature incubator 8 or a variable temperature incubator 9 for germination. Specifically, during operation, the base dish 16 is inverted so that the culture dishes 19 face upwards on the dish holder 15, facilitating the placement of seeds into the culture dishes 19 for cultivation.

[0047] To improve the convenience of experimental operations, the telescopic support rod 5 adopts a telescopic structure, specifically comprising a telescopic rod 20 and a fixed rod 21. One end of the telescopic rod 20 is fixedly connected to the dish holder 15, supporting and driving the movement of the dish holder 15 and the sample dish 3. The other end of the telescopic rod 20 is slidably inserted into the fixed rod 21, allowing the telescopic rod 20 to extend and retract along the axial direction of the fixed rod 21, thereby achieving length adjustment of the entire telescopic support rod 5. In practical use, it extends to bring the sample dish 3 into the parameter environment chamber 1, and retracts to remove the sample dish 3 from the parameter environment chamber 1. Then, by rotating the telescopic support rod, the sample dish 3 is moved to the next parameter environment chamber 1.

[0048] like Figure 5 As shown, one end of the fixed rod 21 is slidably connected to the telescopic rod 20, and the other end of the fixed rod 21 is fixedly connected to the outer wall of the central base column 4 through a rotating connection structure, ensuring the stability of the connection between the fixed rod 21 and the central base column 4. Inside the fixed rod 21, a servo motor 22 is slidably arranged along its axial direction. The servo motor 22 and the inner wall of the fixed rod 21 adopt a sliding fit structure, and the output end of the servo motor 22 is fixedly connected to the telescopic support rod 5 (specifically, the end of the telescopic rod 20 away from the dish 15). The servo motor 22 can provide precise driving force to realize the rotation of the telescopic support rod 5 around its own axis, thereby driving the sample dish 3 to rotate. This is to adapt to the operation actions in specific experimental operations.

[0049] Specifically, for example, after sample dish 3 is removed from the disinfectant box 13, it usually needs to be drained before entering the next parameter environment chamber 1. At this time, the servo motor 22 can drive the sample dish 3 to rotate back and forth at a small angle, and the centrifugal force can be used to dry it, improving experimental efficiency. Another example is when sample dish 3 needs to be layered with sand. The servo motor 22 can be used to rotate the sample dish 3 upside down and put it into the sand layering box 14 for sand filling. When cleaning the sand inside after layering, the sand can also be quickly removed by rotating and shaking. With the low temperature layering box 7, the sand removal and cleaning are completed inside, which not only has a high degree of integration of operation steps, but also makes it less likely for sand to be scattered on the outside.

[0050] To further enhance the functionality of the disinfection box for immersing the sample dish 3, the telescopic rod 20 includes a straight rod body 23 made of a rigid, high-strength material to ensure sufficient support strength for stably supporting the dish box 15 and the sample dish 3. One end of the straight rod body 23 is slidably connected to a fixed rod 21 and can extend and retract along the axial direction of the fixed rod 21. The other end of the straight rod body 23 is fixedly connected to a bent rod head 24, which has a bent structure and extends downwards. The lower end of the bent rod head 24 is fixedly connected to the dish box 15, allowing the dish box 15 to be in a vertically downward position, ensuring that the sample in the sample dish 3 will not tip over and facilitating the entry of the sample dish 3 into the parameter environment chamber 1. The disinfectant container 13 is vertically slidable within the disinfection chamber 6, meaning it can slide up and down along the vertical direction of the chamber 6. A sliding connection structure (such as a slide rail and slider mechanism) is used between the disinfectant container 13 and the inner wall of the chamber 6, allowing the height of the disinfectant container 13 to be flexibly adjusted. By adjusting the height of the disinfectant container 13, and in conjunction with the downward-positioned sample dish 3, the disinfectant container 13 can move upwards to completely enclose the horizontally positioned sample dish 3, ensuring that the sample can fully contact the disinfectant within the container and improving the disinfection effect.

[0051] The above embodiments are merely exemplary embodiments of this application and are not intended to limit this application. The scope of protection of this application is defined by the claims. Those skilled in the art can make various modifications or equivalent substitutions to this application within its substance and scope of protection, and such modifications or equivalent substitutions should also be considered to fall within the scope of protection of this application.

Claims

1. A multi-parameter integrated high-efficiency synergistic germination device, characterized in that, include: Parameter environment box (1), multiple parameter environment boxes (1) are arranged in a ring to form an operating position (2) for placing sample dish (3); A central column (4) is located within the operating position (2). Multiple telescopic support rods (5) are provided on the outer wall of the central column (4). The multiple telescopic support rods (5) are arranged linearly and vertically along the axial direction of the central column (4). The telescopic support rods (5) are all telescopically extended and retracted along the radial direction of the central column (4) toward the parameter environment box (1). A sample dish (3) is provided at the far end of each telescopic support rod (5). The proximal end of the telescopic support rod (5) is rotatably arranged on the outer wall of the central column (4) around the axis of the central column (4). The telescopic support rod rotates around the axis of the central base column (4) to drive the sample dish (3) to circulate in different parameter environment chambers (1); Each of the parameter environment boxes (1) can serve as a parameter path for the sample dish (3) to enter the germination chamber; Two or more of the parameter environment boxes (1) are used in combination according to a preset entry order to work together as a parameter path for the sample dish (3) to germinate.

2. The multi-parameter integrated high-efficiency synergistic germination device according to claim 1, characterized in that: The parameter environment chamber (1) includes at least a sterilization chamber (6), a low-temperature stratification chamber (7), a constant-temperature incubator (8), and a variable-temperature incubator (9). The sterilization chamber (6), the low-temperature stratification chamber (7), the constant-temperature incubator (8), and the variable-temperature incubator (9) are combined to form eight effective parameter paths. The eight effective parameter paths are as follows: Separate germination in constant temperature incubator (8); Separate germination in a variable temperature incubator (9); They are sequentially placed into a low-temperature stratification box (7) and a constant-temperature incubation box for synergistic germination; They are sequentially placed into a low-temperature stratification box (7) and a variable-temperature incubator (9) for synergistic germination. They are sequentially placed into a sterilization box (6) and a constant temperature incubator (8) for synergistic germination; They are sequentially placed into a sterilization box (6) and a variable temperature incubator (9) for synergistic germination; The germination process involves sequentially entering a sterilization box (6), a low-temperature stratification box (7), and a constant-temperature incubator (8) for synergistic germination. The cells are sequentially placed in a sterilization box (6), a low-temperature stratification box (7), and a variable-temperature incubator (9) for synergistic germination. The telescopic support rod (5) is provided in eight parts, and each telescopic support rod (5) participates in a separate parameter path.

3. The multi-parameter integrated high-efficiency synergistic germination device according to claim 2, characterized in that: The doors of the sterilization box (6), the low temperature lamination box (7), the constant temperature incubator (8) and the variable temperature incubator (9) are all provided with opening structures (10), and the telescopic support rod (5) sends the sample dish (3) into the parameter environment box (1) through the opening structure (10). The opening structure (10) includes a through opening (11) through the box door and a closing door (12) provided on the box door. The closing door (12) is slidably disposed on both sides of the through opening (11). The closing door (12) closes the through opening (11) by sliding relative to each other from both sides of the telescopic support rod (5).

4. The multi-parameter integrated high-efficiency synergistic germination device according to claim 3, characterized in that: The disinfection box (6) and the low-temperature stratification box (7) are each equipped with four disinfectant boxes (13) and four stratification sand boxes (14), and each of the four disinfectant boxes (13) and the four stratification sand boxes (14) corresponds to one of the sample dishes (3).

5. The multi-parameter integrated high-efficiency synergistic germination device according to claim 4, characterized in that: The distal end of the telescopic support rod (5) is provided with a dish box (15), and the sample dish (3) is movably disposed in the dish box (15); The sample dish (3) includes a base dish (16) and a lid (17). Both the base dish (16) and the lid (17) are provided with dense flow holes. The base dish (16) is detachably disposed in the dish box (15) for loading the sample. The lid (17) is movably covered on the base dish (16). The dish box (15) is provided with a limiting member to prevent the lid (17) from falling out of the base dish (16).

6. The multi-parameter integrated high-efficiency synergistic germination device according to claim 5, characterized in that: Multiple partition plates (18) are fixedly arranged on the bottom surface of the substrate (16). The partition plates (18) extend downward and protrude from the outer bottom surface of the substrate (16). The multiple partition plates (18) divide the bottom surface of the substrate (16) into multiple culture media (19) for placing samples. The culture media (19) are used to be stored in the constant temperature incubator (8) or the variable temperature incubator (9) for cultivation and germination.

7. The multi-parameter integrated high-efficiency synergistic germination device according to claim 6, characterized in that: The telescopic support rod (5) includes a telescopic rod (20) and a fixed rod (21). One end of the telescopic rod (20) is connected to a dish box (15), and the other end is slidably inserted into the fixed rod (21). One end of the fixed rod (21) is connected to the telescopic rod (20), and the other end is connected to the central base column (4). A servo motor (22) is axially slidably disposed inside the fixed rod (21). The output end of the servo motor (22) is connected to the telescopic support rod (5) to drive the telescopic support rod (5) to rotate axially.

8. The multi-parameter integrated high-efficiency synergistic germination device according to claim 7, characterized in that: The telescopic rod (20) includes a straight rod body (23), one end of which is connected to the fixed rod (21), and the other end is fixedly connected to a bent rod head (24), which extends downward to connect to the dish box (15). The disinfectant box (13) is longitudinally slidably disposed inside the disinfection box (6).

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