A quick-rotting organic material nutrient release accelerator and a preparation method thereof

Abstract

This invention discloses a rapid decomposition nutrient release promoter for organic materials and its preparation method, relating to the field of biology. This rapid decomposition nutrient release promoter and its preparation method overcome the limitations of traditional composting agents, achieving a dual improvement in decomposition efficiency and temperature adaptability. Through the synergistic action of psychrophilic functional bacteria, exogenous complex enzymes, and redox electron mediators, decomposition can be initiated within 24 hours at a low temperature of 5℃, and the pile temperature can rise to above 55℃ within 48 hours at temperatures above 10℃. Under normal temperature conditions, the decomposition cycle of organic materials is shortened from 30-45 days to 12-15 days, with a total degradation rate of lignocellulose ≥75%, an improvement of more than 65% compared to conventional microbial agents. The degree of composting fully meets relevant industry standards, effectively solving the industry pain points of difficult fermentation start-up and long cycles in northern autumn and winter seasons, and significantly reducing the cost of organic solid waste treatment.

Description

Technical Field

[0001] This invention relates to biotechnology, specifically to a rapid decomposition nutrient release promoter for organic materials and its preparation method. Background Technology

[0002] Resource utilization of organic solid waste (straw, livestock and poultry manure, kitchen waste, mushroom residue, etc.) is a key path to practicing the concept of green development and solving environmental pollution and resource waste. Its core step is aerobic decomposition and maturation, and decomposition accelerators, as the core auxiliary agents in this process, directly determine the decomposition efficiency and product quality. Currently, commercial decomposition and maturation agents are widely used in the field of organic solid waste treatment. However, due to technological limitations, there are still many industry pain points that urgently need to be addressed, severely restricting the large-scale and efficient advancement of organic solid waste resource utilization and making it difficult to meet actual production needs. Among these, poor low-temperature adaptability is a prominent problem. Conventional microbial agents can only initiate decomposition above 15℃, resulting in slow fermentation initiation in northern autumn and winter seasons, with a cycle as long as 30-60 days. In some areas, maturation cannot even be completed, significantly increasing treatment costs.

[0003] In addition, existing composting agents have shortcomings such as limited functionality, low efficiency, and significant environmental risks: most products are mainly based on cellulose-degrading bacteria and lack efficient lignin degradation function, with a total lignin cellulose degradation rate of less than 45%, making it difficult to compost materials that are difficult to degrade; decomposition and nutrient release are not synchronized, with nitrogen volatilization loss exceeding 25% during composting, a large amount of phosphorus and potassium fixed, and a low proportion of readily available nutrients in the composting products, resulting in a significant reduction in fertilizer efficiency; they only have basic decomposition functions and cannot simultaneously control odors, degrade antibiotic residues, or passivate heavy metals, which can easily cause secondary pollution; at the same time, the antagonistic effect of multiple strains is strong after compounding, resulting in low retention rate of live bacteria and short shelf life when stored at room temperature, making them poorly adaptable to industrial production and practical application scenarios.

[0004] It is evident that the current application of decomposition accelerators is relatively narrow, with poor adaptability and lack of universal applicability. Developing a new type of decomposition accelerator that combines multiple functions such as low-temperature rapid start-up, efficient decomposition, nutrient retention, and pollution control has become an urgent need in the field of organic solid waste resource utilization. Summary of the Invention

[0005] The purpose of this invention is to provide an accelerator for the rapid decomposition and nutrient release of organic materials and its preparation method, so as to solve the problems that the accelerators in the prior art have a narrow application scope, poor adaptability, and lack universal applicability.

[0006] To achieve the above objectives, the present invention provides the following technical solution: an organic material rapid decomposition nutrient release promoter, comprising, by mass percentage, 15%-25% functional compound microbial community module, 20%-35% bio-induced catalytic module, 30%-50% nutrient synchronous controlled release and passivation module, and 5%-20% carrier filler;

[0007] The functional complex microbial community module consists of psychrophilic fibrinosporium, Phanerochaete chrysosporium, Bacillus laterosporus, Bacillus amyloliquefaciens, Bacillus mucilaginosus, Rhodopseudomonas palustris, Enterococcus faecalis, and Bacillus megaterium.

[0008] The bio-induced catalytic module consists of a compound enzyme preparation, a plant-derived inducer, a redox electron mediator, and a compound nutrient.

[0009] The nutrient synchronous controlled release and passivation module consists of a modified nitrogen storage agent, a phosphorus and potassium activator, a heavy metal passivator, and a pH buffer system.

[0010] The carrier filler includes corn starch and diatomaceous earth.

[0011] Preferably, within the functional complex microbial community module, the relative mass ratio of psychrophilic fibrinolyticus, Phanerochaete chrysosporium, Bacillus laterosporus, Bacillus amyloliquefaciens, Bacillus mucilaginosus, Rhodopseudomonas palustris, Enterococcus faecalis, and Bacillus megaterium is (15%-20%): (10%-15%): (15%-20%): (10%-15%): (5%-10%): (10%-15%): (5%-10%).

[0012] Preferably, in the bio-induced catalytic module, the mass ratio of the compound enzyme preparation, plant-derived inducer, redox electron mediator, and compound nutrient to the total system is (4%-8%): (3%-6%): (2%-5%): (11%-16%).

[0013] Preferably, in the nutrient synchronous controlled release and passivation module, the modified nitrogen storage agent, phosphorus and potassium activator, heavy metal passivator, and pH buffer system account for (8%-15%): (5%-10%): (10%-18%): (7%-12%) of the total system by mass. The heavy metal passivator is sodium sulfide modified nano-biochar, and the pH buffer system is composed of 60% food-grade calcium carbonate, 25% potassium dihydrogen phosphate, and 15% dipotassium hydrogen phosphate by mass.

[0014] Preferably, the corn starch and diatomaceous earth in the carrier filler are mixed in a 1:1 mass ratio.

[0015] A method for preparing a nutrient release accelerator for rapid decomposition of organic materials, applicable to the preparation of accelerators, comprising the following steps:

[0016] The psychrophilic filamentous bacteria, *Phanerochaete chrysosporium*, *Bacillus laterosporus*, *Bacillus amyloliquefaciens*, *Bacillus mucilaginosus*, *Rhodopseudomonas palustris*, *Enterococcus faecalis*, and *Bacillus megaterium* used to prepare the functional complex microbial community module were pretreated to obtain bacterial fermentation broth, fungal fermentation broth, and photosynthetic bacterial fermentation broth. The three fermentation broths were mixed with sodium alginate and corn starch to obtain a mixed bacterial suspension in which the strains were microcapsules were embedded. After drying, a complex microbial community powder was obtained, which served as the functional complex microbial community module.

[0017] A modified nitrogen storage agent was prepared by mixing attapulgite powder, silane coupling agent, and tea polyphenols; sodium sulfide-modified nano-biochar was prepared by mixing peanut shell biochar and sodium sulfide; a heavy metal passivator was prepared by mixing sodium sulfide-modified nano-biochar and nano-hydroxyapatite; a bio-induced catalytic module was prepared by mixing compound enzyme preparation, plant-derived inducer, electron mediator, and compound nutrient agent; and a nutrient synchronous controlled release and passivation module was prepared by mixing modified nitrogen storage agent, phosphorus and potassium activator, heavy metal passivator, and pH buffer system.

[0018] Add the carrier filler to the mixer, then add the nutrient synchronous controlled release and passivation module, and mix at 25℃ and 60rpm for 15min; add the catalytic module premix and continue mixing for 10min; finally add the compound microbial powder, and mix at 25℃, in the dark, and at a low speed of 40rpm for 20min to ensure that the coefficient of variation of the mixing uniformity is ≤5%; after mixing, vacuum package under dark, dry, and room temperature conditions to obtain the finished product accelerator.

[0019] Preferably, the preparation steps of the functional complex microbial community module include:

[0020] Each strain was inoculated into the corresponding slant culture medium. Bacteria were cultured at 30℃ for 24 hours, fungi at 28℃ for 48 hours, and photosynthetic bacteria were cultured at 30℃ and 3000 lux light for 72 hours to obtain purified and activated strains.

[0021] The activated strains were inoculated into seed culture medium. The bacteria were cultured at 30°C and 180 rpm for 24 h, the fungi were cultured at 28°C and 150 rpm for 36 h, and the photosynthetic bacteria were cultured at 30°C under static light for 72 h to obtain seed liquid with OD600≥1.2.

[0022] Bacterial seed cultures of *Cyclophila*, *Bacillus laterosporus*, *Bacillus amyloliquefaciens*, *Bacillus mucilaginosus*, *Enterococcus faecalis*, and *Bacillus megaterium* were inoculated into fermentation medium at a ratio of 5%, fermentation temperature of 30℃, aeration ratio of 1:0.8, tank pressure of 0.05 MPa, stirring speed of 180 rpm, and fermentation time of 24 h to obtain bacterial fermentation broth. *Phanerochaete chrysosporium* seed culture was inoculated into fungal-specific medium at a ratio of 8%, fermentation temperature of 28℃, aeration ratio of 1:0.6, tank pressure of 0.05 MPa, stirring speed of 120 rpm, and fermentation time of 36 h to obtain fungal fermentation broth. *Rhodopseudomonas palustris* seed culture was inoculated into photosynthetic medium at a ratio of 10%, and cultured statically at 30℃ and 3000 lux light for 72 h to obtain photosynthetic bacterial fermentation broth.

[0023] The three fermentation broths were mixed according to the formula ratio, and 2% sodium alginate and 1% corn starch were added to the total mass of the fermentation broth. The mixture was stirred at low speed at room temperature for 30 minutes to obtain a uniform mixed bacterial suspension, thereby achieving microcapsule encapsulation of the bacterial strains and improving stress resistance and storage stability.

[0024] The bacterial suspension was subjected to low-temperature spray drying with an inlet air temperature of 120℃, an outlet air temperature of 55℃, and an atomization pressure of 0.3MPa, yielding a viable bacterial count ≥2.0×10⁻⁶. 10 CFU / g compound microbial powder, stored in a light-proof and sealed container.

[0025] Preferably, the preparation steps of the nutrient simultaneous controlled release and passivation module include:

[0026] Attapulgite soil was crushed and passed through a 200-mesh sieve. 2% of the mass of the attapulgite soil was added to the silane coupling agent KH550. The mixture was stirred in a high-speed mixer at 80°C and 1200 rpm for 30 minutes. After cooling, tea polyphenols were added and mixed evenly to obtain a modified nitrogen retention agent for later use.

[0027] Peanut shell biochar was pulverized and passed through a 300-mesh sieve. Sodium sulfide, accounting for 5% of the biochar mass, was added at a solid-liquid ratio of 1:10. The mixture was reacted in a hydrothermal reactor at 180°C for 6 hours. After cooling and washing until neutral, the mixture was dried and pulverized to obtain sodium sulfide-modified nano-biochar. The nano-biochar was then mixed with nano-hydroxyapatite at high speed according to the specified ratio to obtain a heavy metal passivating agent for later use.

[0028] The modified nitrogen storage agent, phosphorus and potassium activator, heavy metal passivator, and pH buffer system were mixed at 25°C and 1000 rpm for 30 minutes according to the specified ratio to obtain the controlled-release module premix, which was then set aside for later use.

[0029] Preferably, the compound enzyme preparation, plant-derived inducer, electron mediator, and compound nutrient are mixed at a low speed of 25°C and 800 rpm for 20 minutes according to the specified ratio to obtain the catalytic module premix.

[0030] Compared with existing technologies, this invention provides a rapid decomposition nutrient release promoter for organic materials and its preparation method, breaking through the limitations of traditional composting agents and achieving a dual improvement in decomposition efficiency and temperature adaptability. Through the synergistic effect of psychrophilic functional bacteria, exogenous complex enzymes, and redox electron mediators, decomposition can be initiated within 24 hours at a low temperature of 5℃, and the pile temperature can rise to above 55℃ within 48 hours at temperatures above 10℃. Under normal temperature conditions, the decomposition cycle of organic materials is shortened from 30-45 days to 12-15 days, the total degradation rate of lignocellulose is ≥75%, which is more than 65% higher than that of conventional microbial agents, and the degree of decomposition fully meets relevant industry standards. This effectively solves the industry pain points of difficult fermentation start-up and long cycle in northern autumn and winter seasons, and significantly reduces the cost of organic solid waste treatment.

[0031] This accelerator combines nutrient retention, pollution control, and high stability, resulting in outstanding overall benefits. The modified nitrogen retention agent, in synergy with functional bacteria, reduces nitrogen volatilization loss in compost by ≥42%, increases phosphorus and potassium activation rates by over 58% and 45% respectively, and significantly improves the content of readily available nutrients in the composting products. Simultaneously, it controls odor, degrades antibiotics, and passivates heavy metals, significantly reducing ammonia and hydrogen sulfide release, achieving an antibiotic degradation rate of ≥95%, and reducing the content of available heavy metals by over 62%. The product utilizes microencapsulation technology, has a shelf life of ≥12 months at room temperature, is compatible with various organic materials and composting processes, and is green, low-cost, and highly adaptable to industrial applications. Attached Figure Description

[0032] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0033] Figure 1 A block diagram of the accelerator components provided in the embodiments of the present invention;

[0034] Figure 2 This is a flowchart illustrating the accelerator preparation method provided in an embodiment of the present invention. Detailed Implementation

[0035] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0036] As attached Figure 1 To be continued Figure 2 As shown:

[0037] Example:

[0038] This invention provides an organic material rapid decomposition nutrient release promoter, which, by mass percentage, comprises 15%-25% functional compound microbial community module, 20%-35% bio-induced catalytic module, 30%-50% nutrient synchronous controlled release and passivation module, and 5%-20% carrier filler;

[0039] The functional complex microbial community module consists of psychrophilic fibrinosporium, Phanerochaete chrysosporium, Bacillus laterosporus, Bacillus amyloliquefaciens, Bacillus mucilaginosus, Rhodopseudomonas palustris, Enterococcus faecalis, and Bacillus megaterium.

[0040] The bio-induced catalytic module consists of a compound enzyme preparation, a plant-derived inducer, a redox electron mediator, and a compound nutrient.

[0041] The nutrient synchronous controlled release and passivation module consists of a modified nitrogen storage agent, a phosphorus and potassium activator, a heavy metal passivator, and a pH buffer system.

[0042] The carrier filler includes corn starch and diatomaceous earth.

[0043] As shown above, this accelerator overcomes the limitations of traditional composting agents, achieving a dual improvement in decomposition efficiency and temperature adaptability. Through the synergistic effect of psychrophilic functional bacteria, exogenous complex enzymes, and redox electron mediators, decomposition can be initiated within 24 hours at a low temperature of 5℃, and the pile temperature can rise to above 55℃ within 48 hours at temperatures above 10℃. Under normal temperature conditions, the decomposition cycle of organic materials is shortened from 30-45 days to 12-15 days, the total degradation rate of lignocellulose is ≥75%, which is more than 65% higher than that of conventional microbial agents, and the degree of decomposition fully meets relevant industry standards. This effectively solves the industry pain points of difficult fermentation start-up and long cycle in northern autumn and winter seasons, and significantly reduces the cost of organic solid waste treatment.

[0044] This accelerator combines nutrient retention, pollution control, and high stability, resulting in outstanding overall benefits. The modified nitrogen retention agent, in synergy with functional bacteria, reduces nitrogen volatilization loss in compost by ≥42%, increases phosphorus and potassium activation rates by over 58% and 45% respectively, and significantly improves the content of readily available nutrients in the composting products. Simultaneously, it controls odor, degrades antibiotics, and passivates heavy metals, significantly reducing ammonia and hydrogen sulfide release, achieving an antibiotic degradation rate of ≥95%, and reducing the content of available heavy metals by over 62%. The product utilizes microencapsulation technology, has a shelf life of ≥12 months at room temperature, is compatible with various organic materials and composting processes, and is green, low-cost, and highly adaptable to industrial applications.

[0045] Within the functional complex microbial community module, the relative mass ratio of psychrophilic fibrinolyticus, Phanerochaete chrysosporium, Bacillus laterosporus, Bacillus amyloliquefaciens, Bacillus mucilaginosus, Rhodopseudomonas palustris, Enterococcus faecalis, and Bacillus megaterium is (15%-20%): (10%-15%): (15%-20%): (10%-15%): (5%-10%): (10%-15%): (5%-10%): (5%-10%).

[0046] Psychrophilic fibrinolyticus viable count ≥ 5.0 × 10⁻⁶ 9 CFU / g, low-temperature start-up at 5℃, highly efficient cellulose degradation; viable count of *Phanerochaete chrysosporium* ≥ 2.0 × 10⁻⁶ 9 CFU / g, highly efficient at degrading lignin, breaking through the rate-limiting step of decomposition; viable count of Bacillus retrosporum ≥3.0×10⁻⁶ 9 CFU / g, degrades lignocellulose, inhibits pathogens; Bacillus amyloliquefaciens viable count ≥3.0×10⁻⁶ 9 CFU / g, degrades protein / pectin, produces antibacterial substances; viable Bacillus mucilaginosus count ≥1.0×10⁻⁶ 9 CFU / g, activates phosphorus and potassium, decomposes silicate minerals; viable count of Rhodopseudomonas palustris ≥2.0×10⁻⁶ 9 CFU / g, deodorizing, antibiotic degradation, nitrogen fixation; Enterococcus faecalis viable count ≥2.0×10⁻⁶ 9 CFU / g, inhibits odor-producing bacteria, degrades tetracycline antibiotics; Bacillus megaterium viable count ≥1.0×10⁻⁶ 9 CFU / g, activated phosphorus, passivates heavy metals.

[0047] In the bio-induced catalytic module, the mass ratio of compound enzyme preparation, plant-derived inducer, redox electron mediator, and compound nutrient to the total system is (4%-8%): (3%-6%): (2%-5%): (11%-16%).

[0048] In the compound enzyme preparation, cellulase ≥10000U / g, lignin peroxidase ≥5000U / g, manganese peroxidase ≥3000U / g, chitinase ≥2000U / g, and neutral protease ≥8000U / g are present. Exogenous enzymes rapidly initiate decomposition and synergistically enhance the effects with endogenous enzymes. In the plant-derived inducer, Ginkgo biloba leaf flavonoid extract (flavonoids ≥80%) 60% + tea saponin (≥90%) 40% are present. This induces quorum sensing and colonization of the microbial community, enhances enzyme production, and inhibits bacteria and deodorizes. In the redox electron mediator, anthraquinone-2-sulfonate sodium (AQS) 40% + high-quinone modified humic acid (quinone group ≥1.2mmol / g) 60% are present. This accelerates extracellular electron transfer in the degradation of lignocellulose and increases the decomposition rate by more than 60%. In the compound nutrient, yeast extract 85% + biotin 5% + VB1 5% + VB6 5% are present. This provides rapid-acting nutrition for microbial community proliferation and shortens the lag phase.

[0049] In the nutrient synchronous controlled release and passivation module, the modified nitrogen storage agent, phosphorus and potassium activator, heavy metal passivator, and pH buffer system account for (8%-15%): (5%-10%): (10%-18%): (7%-12%) of the total system by mass. The heavy metal passivator is sodium sulfide modified nano-biochar, and the pH buffer system is composed of 60% food-grade calcium carbonate, 25% potassium dihydrogen phosphate, and 15% dipotassium hydrogen phosphate by mass.

[0050] The phosphorus and potassium activator contains 60% potassium humate (humic acid ≥60%, K2O ≥10%) and 40% food-grade citric acid; it chelates metal ions, activates fixed phosphorus and potassium, and improves nutrient availability.

[0051] The carrier filler contains corn starch and diatomaceous earth mixed in a 1:1 mass ratio.

[0052] A method for preparing a nutrient release accelerator for rapid decomposition of organic materials, applicable to the preparation of accelerators, comprising the following steps:

[0053] The psychrophilic filamentous bacteria, *Phanerochaete chrysosporium*, *Bacillus laterosporus*, *Bacillus amyloliquefaciens*, *Bacillus mucilaginosus*, *Rhodopseudomonas palustris*, *Enterococcus faecalis*, and *Bacillus megaterium* used to prepare the functional complex microbial community module were pretreated to obtain bacterial fermentation broth, fungal fermentation broth, and photosynthetic bacterial fermentation broth. The three fermentation broths were mixed with sodium alginate and corn starch to obtain a mixed bacterial suspension in which the strains were microcapsules were embedded. After drying, a complex microbial community powder was obtained, which served as the functional complex microbial community module.

[0054] A modified nitrogen storage agent was prepared by mixing attapulgite powder, silane coupling agent, and tea polyphenols; sodium sulfide-modified nano-biochar was prepared by mixing peanut shell biochar and sodium sulfide; a heavy metal passivator was prepared by mixing sodium sulfide-modified nano-biochar and nano-hydroxyapatite; a bio-induced catalytic module was prepared by mixing compound enzyme preparation, plant-derived inducer, electron mediator, and compound nutrient agent; and a nutrient synchronous controlled release and passivation module was prepared by mixing modified nitrogen storage agent, phosphorus and potassium activator, heavy metal passivator, and pH buffer system.

[0055] Add the carrier filler to the mixer, then add the nutrient synchronous controlled release and passivation module, and mix at 25℃ and 60rpm for 15min; add the catalytic module premix and continue mixing for 10min; finally add the compound microbial powder, and mix at 25℃, in the dark, and at a low speed of 40rpm for 20min to ensure that the coefficient of variation of the mixing uniformity is ≤5%; after mixing, vacuum package under dark, dry, and room temperature conditions to obtain the finished product accelerator.

[0056] The preparation steps of the functional complex microbial community module include:

[0057] Each strain was inoculated into the corresponding slant culture medium. Bacteria were cultured at 30℃ for 24 hours, fungi at 28℃ for 48 hours, and photosynthetic bacteria were cultured at 30℃ and 3000 lux light for 72 hours to obtain purified and activated strains.

[0058] The activated strains were inoculated into seed culture medium. The bacteria were cultured at 30°C and 180 rpm for 24 h, the fungi were cultured at 28°C and 150 rpm for 36 h, and the photosynthetic bacteria were cultured at 30°C under static light for 72 h to obtain seed liquid with OD600≥1.2.

[0059] Bacterial seed cultures of *Cyclophila*, *Bacillus laterosporus*, *Bacillus amyloliquefaciens*, *Bacillus mucilaginosus*, *Enterococcus faecalis*, and *Bacillus megaterium* were inoculated into fermentation medium at a ratio of 5%, fermentation temperature of 30℃, aeration ratio of 1:0.8, tank pressure of 0.05 MPa, stirring speed of 180 rpm, and fermentation time of 24 h to obtain bacterial fermentation broth. *Phanerochaete chrysosporium* seed culture was inoculated into fungal-specific medium at a ratio of 8%, fermentation temperature of 28℃, aeration ratio of 1:0.6, tank pressure of 0.05 MPa, stirring speed of 120 rpm, and fermentation time of 36 h to obtain fungal fermentation broth. *Rhodopseudomonas palustris* seed culture was inoculated into photosynthetic medium at a ratio of 10%, and cultured statically at 30℃ and 3000 lux light for 72 h to obtain photosynthetic bacterial fermentation broth.

[0060] The three fermentation broths were mixed according to the formula ratio, and 2% sodium alginate and 1% corn starch were added to the total mass of the fermentation broth. The mixture was stirred at low speed at room temperature for 30 minutes to obtain a uniform mixed bacterial suspension, thereby achieving microcapsule encapsulation of the bacterial strains and improving stress resistance and storage stability.

[0061] The bacterial suspension was subjected to low-temperature spray drying with an inlet air temperature of 120℃, an outlet air temperature of 55℃, and an atomization pressure of 0.3MPa, yielding a viable bacterial count ≥2.0×10⁻⁶. 10 CFU / g compound microbial powder, stored in a light-proof and sealed container.

[0062] The preparation steps of the nutrient synchronous controlled release and passivation module include:

[0063] Attapulgite soil was crushed and passed through a 200-mesh sieve. 2% of the mass of the attapulgite soil was added to the silane coupling agent KH550. The mixture was stirred in a high-speed mixer at 80°C and 1200 rpm for 30 minutes. After cooling, tea polyphenols were added and mixed evenly to obtain a modified nitrogen retention agent for later use.

[0064] Peanut shell biochar was pulverized and passed through a 300-mesh sieve. Sodium sulfide, accounting for 5% of the biochar mass, was added at a solid-liquid ratio of 1:10. The mixture was reacted in a hydrothermal reactor at 180°C for 6 hours. After cooling and washing until neutral, the mixture was dried and pulverized to obtain sodium sulfide-modified nano-biochar. The nano-biochar was then mixed with nano-hydroxyapatite at high speed according to the specified ratio to obtain a heavy metal passivating agent for later use.

[0065] The modified nitrogen storage agent, phosphorus and potassium activator, heavy metal passivator, and pH buffer system were mixed at 25°C and 1000 rpm for 30 minutes according to the specified ratio to obtain the controlled-release module premix, which was then set aside for later use.

[0066] The compound enzyme preparation, plant-derived inducer, electron mediator, and compound nutrient were mixed at a low speed of 800 rpm for 20 minutes at 25°C to obtain the catalytic module premix.

[0067] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A nutrient release promoter for rapid decomposition of organic materials, characterized in that, By mass percentage, the components include 15%–25% functional complex microbial community module, 20%–35% bio-induced catalysis module, 30%–50% nutrient synchronous controlled release and passivation module, and 5%–20% carrier filler; The functional complex microbial community module consists of psychrophilic fibrinosporium, Phanerochaete chrysosporium, Bacillus laterosporus, Bacillus amyloliquefaciens, Bacillus mucilaginosus, Rhodopseudomonas palustris, Enterococcus faecalis, and Bacillus megaterium. The bio-induced catalytic module consists of a compound enzyme preparation, a plant-derived inducer, a redox electron mediator, and a compound nutrient. The nutrient synchronous controlled release and passivation module consists of a modified nitrogen storage agent, a phosphorus and potassium activator, a heavy metal passivator, and a pH buffer system. The carrier filler includes corn starch and diatomaceous earth.

2. The organic material rapid decomposition nutrient release promoter according to claim 1, characterized in that, Within the functional complex microbial community module, the relative mass ratio of psychrophilic fibrinolyticus, Phanerochaete chrysosporium, Bacillus laterosporus, Bacillus amyloliquefaciens, Bacillus mucilaginosus, Rhodopseudomonas palustris, Enterococcus faecalis, and Bacillus megaterium is (15%-20%): (10%-15%): (15%-20%): (10%-15%): (5%-10%): (10%-15%): (5%-10%): (5%-10%).

3. The organic material rapid decomposition nutrient release promoter according to claim 1, characterized in that, In the bio-induced catalytic module, the mass ratio of compound enzyme preparation, plant-derived inducer, redox electron mediator, and compound nutrient to the total system is (4%-8%): (3%-6%): (2%-5%): (11%-16%).

4. The organic material rapid decomposition nutrient release promoter according to claim 1, characterized in that, In the nutrient synchronous controlled release and passivation module, the modified nitrogen storage agent, phosphorus and potassium activator, heavy metal passivator, and pH buffer system account for (8%-15%): (5%-10%): (10%-18%): (7%-12%) of the total system by mass. The heavy metal passivator is sodium sulfide modified nano-biochar, and the pH buffer system is composed of 60% food-grade calcium carbonate, 25% potassium dihydrogen phosphate, and 15% dipotassium hydrogen phosphate by mass.

5. The organic material rapid decomposition nutrient release promoter according to claim 1, characterized in that, The carrier filler contains corn starch and diatomaceous earth mixed in a 1:1 mass ratio.

6. A method for preparing a nutrient release promoter for rapid decomposition of organic materials, characterized in that, The step of preparing the accelerator as described in any one of claims 1-5 includes: The psychrophilic filamentous bacteria, *Phanerochaete chrysosporium*, *Bacillus laterosporus*, *Bacillus amyloliquefaciens*, *Bacillus mucilaginosus*, *Rhodopseudomonas palustris*, *Enterococcus faecalis*, and *Bacillus megaterium* used to prepare the functional complex microbial community module were pretreated to obtain bacterial fermentation broth, fungal fermentation broth, and photosynthetic bacterial fermentation broth. The three fermentation broths were mixed with sodium alginate and corn starch to obtain a mixed bacterial suspension in which the strains were microcapsules were embedded. After drying, a complex microbial community powder was obtained, which served as the functional complex microbial community module. A modified nitrogen storage agent was prepared by mixing attapulgite powder, silane coupling agent, and tea polyphenols; sodium sulfide-modified nano-biochar was prepared by mixing peanut shell biochar and sodium sulfide; a heavy metal passivator was prepared by mixing sodium sulfide-modified nano-biochar and nano-hydroxyapatite; a bio-induced catalytic module was prepared by mixing compound enzyme preparation, plant-derived inducer, electron mediator, and compound nutrient agent; and a nutrient synchronous controlled release and passivation module was prepared by mixing modified nitrogen storage agent, phosphorus and potassium activator, heavy metal passivator, and pH buffer system. Add the carrier filler to the mixer, then add the nutrient synchronous controlled release and passivation module, and mix at 25°C and 60 rpm for 15 min; add the catalytic module premix and continue mixing for 10 min; finally add the compound microbial powder and mix at 25°C, in the dark, and at a low speed of 40 rpm for 20 min; after mixing, vacuum package under dark, dry, and room temperature conditions to obtain the finished product accelerator.

7. The method for preparing a nutrient release promoter for rapid decomposition of organic materials according to claim 6, characterized in that, The preparation steps of the functional complex microbial community module include: Each strain was inoculated into the corresponding slant culture medium. Bacteria were cultured at 30℃ for 24 hours, fungi at 28℃ for 48 hours, and photosynthetic bacteria were cultured at 30℃ and 3000 lux light for 72 hours to obtain purified and activated strains. The activated strains were inoculated into seed culture medium. The bacteria were cultured at 30°C and 180 rpm for 24 h, the fungi were cultured at 28°C and 150 rpm for 36 h, and the photosynthetic bacteria were cultured at 30°C under static light for 72 h to obtain seed liquid with OD600≥1.

2. Bacterial seed cultures of *Cyclophila*, *Bacillus laterosporus*, *Bacillus amyloliquefaciens*, *Bacillus mucilaginosus*, *Enterococcus faecalis*, and *Bacillus megaterium* were inoculated into fermentation medium at a ratio of 5%, fermentation temperature of 30℃, aeration ratio of 1:0.8, tank pressure of 0.05 MPa, stirring speed of 180 rpm, and fermentation time of 24 h to obtain bacterial fermentation broth. *Phanerochaete chrysosporium* seed culture was inoculated into fungal-specific medium at a ratio of 8%, fermentation temperature of 28℃, aeration ratio of 1:0.6, tank pressure of 0.05 MPa, stirring speed of 120 rpm, and fermentation time of 36 h to obtain fungal fermentation broth. *Rhodopseudomonas palustris* seed culture was inoculated into photosynthetic medium at a ratio of 10%, and cultured statically at 30℃ and 3000 lux light for 72 h to obtain photosynthetic bacterial fermentation broth. The three fermentation broths were mixed according to the formula ratio, and 2% sodium alginate and 1% corn starch were added to the total mass of the fermentation broth. The mixture was stirred at low speed at room temperature for 30 minutes to obtain a uniform mixed bacterial suspension, thus achieving microcapsule encapsulation of the bacterial strains. The bacterial suspension was subjected to low-temperature spray drying with an inlet air temperature of 120℃, an outlet air temperature of 55℃, and an atomization pressure of 0.3MPa, yielding a viable bacterial count ≥2.0×10⁻⁶. 10 CFU / g compound microbial powder, stored in a light-proof and sealed container.

8. A method for preparing a nutrient release promoter for rapid decomposition of organic materials according to claim 6, characterized in that, The preparation steps of the nutrient synchronous controlled release and passivation module include: Attapulgite soil was crushed and passed through a 200-mesh sieve. 2% of the mass of the attapulgite soil was added to the silane coupling agent KH550. The mixture was stirred in a high-speed mixer at 80°C and 1200 rpm for 30 minutes. After cooling, tea polyphenols were added and mixed evenly to obtain a modified nitrogen retention agent for later use. Peanut shell biochar was pulverized and passed through a 300-mesh sieve. Sodium sulfide, accounting for 5% of the biochar mass, was added at a solid-liquid ratio of 1:

10. The mixture was reacted in a hydrothermal reactor at 180°C for 6 hours. After cooling and washing until neutral, the mixture was dried and pulverized to obtain sodium sulfide-modified nano-biochar. The nano-biochar was then mixed with nano-hydroxyapatite at high speed according to the specified ratio to obtain a heavy metal passivating agent for later use. The modified nitrogen storage agent, phosphorus and potassium activator, heavy metal passivator, and pH buffer system were mixed at 25°C and 1000 rpm for 30 minutes according to the specified ratio to obtain the controlled-release module premix, which was then set aside for later use.

9. A method for preparing a nutrient release promoter for rapid decomposition of organic materials according to claim 6, characterized in that, The compound enzyme preparation, plant-derived inducer, electron mediator, and compound nutrient were mixed at a low speed of 800 rpm for 20 minutes at 25°C to obtain the catalytic module premix.

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