Molecular marker associated with seed oil content in soybean GmANK gene and application

By locating the InDel site in the soybean GmANK gene and combining it with PCR amplification and primer combinations, the problems of accuracy and efficiency in identifying soybean seed oil content were solved, enabling early screening of high-oil soybean varieties and shortening the breeding cycle.

CN122235375APending Publication Date: 2026-06-19NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S +1
View PDF 0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
Filing Date
2026-05-22
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

Existing technologies are insufficient for quickly and accurately identifying the oil and protein content of soybean seeds, and traditional breeding methods are time-consuming, making it difficult to meet the breeding needs of high-oil and high-yield soybean varieties.

Method used

Genome-wide association analysis was used to identify the InDel site of the soybean GmANK gene. By inserting or deleting a fragment with the nucleotide sequence GTAGGTTAT, combined with PCR amplification and primer combinations, the oil content of seeds was identified, providing a molecular marker-assisted breeding method.

Benefits of technology

It enables early and rapid screening and prediction of high-oil soybean varieties, shortens the breeding cycle, and improves breeding efficiency.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN122235375A_ABST
    Figure CN122235375A_ABST
Patent Text Reader

Abstract

This invention relates to the field of molecular biology, providing molecular markers in the soybean GmANK gene associated with seed oil content and their applications. The nucleotide sequences of these molecular markers are shown in SEQ ID NO.1 and SEQ ID NO.2. This invention discovered the InDel marker associated with soybean seed oil content through genome-wide association analysis. This marker is located in the promoter region of the GmANK gene on chromosome 17. Using these molecular markers, early and rapid screening of high-oil-content soybean varieties can be achieved. They can be used to predict the oil content in soybean seeds during the seedling stage, providing new technical support for soybean molecular marker-assisted breeding technology, effectively shortening the breeding cycle, improving breeding efficiency, and having practical application value in accelerating the breeding process of new high-oil soybean varieties.
Need to check novelty before this filing date? Find Prior Art