A triple real-time fluorescent quantitative PCR primer probe set, kit and application thereof

By designing a triple real-time fluorescence quantitative PCR primer and probe set, the problem of the inability to simultaneously detect wild-type pseudorabies virus, vaccine strain, and porcine circovirus type 2 in existing technologies has been solved, achieving rapid and accurate triple detection and improving the prevention and control capabilities of the pig industry.

CN122235384APending Publication Date: 2026-06-19WENS FOODSTUFF GROUP CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WENS FOODSTUFF GROUP CO LTD
Filing Date
2026-05-18
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

Current technology lacks primers or kits for triple real-time quantitative PCR that can simultaneously, rapidly, and accurately detect wild-type pseudorabies virus, vaccine strains, and porcine circovirus type 2. Mixed infections lead to increased mortality and prolonged illness in pigs.

Method used

A triplet real-time quantitative PCR primer and probe set was designed, consisting of specific primer and probe sequences, including primer and probe sequences for detecting wild-type pseudorabies virus, vaccine strain, and porcine circovirus type 2. The three can be distinguished and detected in a single PCR reaction, and a specific fluorescent reporter gene is used to distinguish different viruses.

Benefits of technology

It enables rapid and accurate detection of wild-type pseudorabies virus, vaccine strains, and porcine circovirus type 2, simplifies the operation process, improves detection efficiency, provides scientific basis for prevention and treatment strategies, and enhances the prevention and control capabilities of the pig industry.

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Abstract

This invention discloses a triple real-time quantitative PCR primer and probe set, a kit containing the primer and probe set, a detection method, and applications. This primer and probe set can be used to simultaneously detect wild-type pseudorabies virus, vaccine strains, and porcine circovirus type 2 (PCV2) strains. The primer and probe set consists of the sequences shown in SEQ ID NO:1-SEQ ID NO:9. By using this primer and probe set for triple real-time quantitative PCR detection of genomic DNA in the test sample, the presence of wild-type pseudorabies virus, vaccine strains, and PCV2 strains can be detected rapidly and accurately. Furthermore, only one PCR reaction is required to simultaneously detect these three strains, offering advantages such as ease of operation and high detection efficiency. It has significant application value for the prevention and control of pseudorabies and PCV2, as well as for promoting the development of the pig farming industry.
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