Method for quantitatively detecting total phosphate in aquatic product by combining acid hydrolysis with liquid chromatography-tandem mass spectrometry

By combining acid hydrolysis with liquid chromatography-tandem mass spectrometry, the sensitivity and accuracy issues of phosphate detection in aquatic products have been resolved, achieving efficient and accurate quantification of total phosphate, which is suitable for food safety supervision.

CN122238523APending Publication Date: 2026-06-19ZHOUSHAN DISEASE CONTROL & PREVENTION CENT
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Patent Information

Application Number
CN202610366238.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-24
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

Existing technologies for phosphate detection in aquatic products suffer from problems such as insufficient sensitivity, large quantitative deviation, weak resistance to matrix interference, and cumbersome operation, making it difficult to meet the needs of food safety supervision.

Method used

Acid hydrolysis combined with liquid chromatography-tandem mass spectrometry was employed to convert various phosphates into orthophosphates through high-temperature acid hydrolysis, and then accurately determined using liquid chromatography-tandem mass spectrometry. An Acclaim Trinity Q1 column and electrospray ionization source in negative ion mode were selected, combined with orthophosphate isotope internal standards, to optimize chromatographic and mass spectrometric conditions, thereby achieving complete hydrolysis and separation of polyphosphates.

Benefits of technology

It enables accurate determination of total phosphate in aquatic products, reduces the difficulty of detection operations and the cost of consumables, improves detection efficiency and accuracy, adapts to the detection needs of different aquatic product matrices, and has good practical application value.

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Abstract

The invention relates to the technical field of analytical chemistry, in particular to a method for quantitatively detecting total phosphate in an aquatic product by combining acid hydrolysis with liquid chromatography-tandem mass spectrometry. Wherein the orthophosphate is good in linearity when the concentration is 0.1-20.0 mg / L, the correlation coefficient is 0.9991, the detection limit is 0.03 g / kg, the quantitation limit is 0.10 g / kg, the matrix effect is low, and under the standard addition level of 1.0-10.0 g / kg, the intraday recovery rates of the shrimp meat and the fish meat are 89.8%-102.8% and 88.7%-105.8% respectively; the daily recovery rate is 90.9 percent to 106.4 percent, and the daily recovery rate is 90.2 percent to 105.8 percent; the RSD in the day is less than or equal to 10.0%, and the RSD in the day is less than or The method is simple in pretreatment, strong in interference resistance, high in sensitivity, excellent in accuracy and precision, suitable for detecting the total phosphate in the aquatic product, capable of providing a reliable technical means for monitoring the total phosphate in the aquatic product, and strong in practicability.
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