Tbt1 protein, disease-resistant gene, molecular marker for improving resistance to tobrfv and application thereof

Abstract

This invention discloses a TBT1 protein, a resistance gene, a molecular marker, and their applications for enhancing resistance to ToBRFV, belonging to the field of plant resistance gene technology. This invention also identified a novel gene on chromosome 11 of tomato that exhibits resistance to ToBRFV. TBT1 Overexpression in tomatoes TBT1 It can significantly enhance resistance to brown wrinkled fruit virus. Compared to disease-resistant genes, the disease-resistant gene of this invention... TBT1 This invention avoids exerting selective pressure on the virus, effectively preventing viral gene evolution from overcoming resistance and achieving long-term stable disease resistance. It addresses the technical pain points of existing disease resistance genes being prone to loss of resistance and requiring frequent variety updates. Furthermore, this invention has developed two related to disease resistance genes. TBT1 The tightly linked molecular markers of this invention can be used to detect and identify resistance genes for tomato brown wrinkle fruit virus disease. This method can be used in a simple, fast, and high-throughput manner in breeding practice, thus accelerating the process of disease-resistant tomato breeding.

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