Preparation method of antibacterial bacterial cellulose

Abstract

The application relates to a preparation method of antibacterial bacterial cellulose, and comprises the following steps: step one, taking pure coconut water for standby; step two, boiling the diluted coconut water solution to 100 DEG C; step three, adding amino acid additives with a concentration after the coconut water solution is cooled to 70-80 DEG C; step four, maintaining the temperature of the heated coconut water solution at 70-80 DEG C, and adding sodium hydroxide; step five, filtering the heat-preserved coconut water solution, washing the filter residue with distilled water until neutral, and drying to obtain a fiber sample. The application belongs to the technical field of bacterial cellulose, and the preparation method of the antibacterial bacterial cellulose can produce bacterial cellulose by using simple raw materials, and can ensure the redispersibility, structural stability and high quality of the bacterial cellulose.

Description

Technical Field

[0001] This invention belongs to the field of bacterial cellulose technology, specifically referring to a method for preparing antibacterial bacterial cellulose. Background Technology

[0002] Bacterial cellulose is a cellulose hydrogel produced by microbial fermentation. It possesses a fine nanofiber 3D structure, endowing it with various excellent properties, such as extremely high water content, strong adsorption and diffusion capabilities, good elasticity, excellent mechanical properties, and high biocompatibility. It has wide applications in food, biomedicine, chemical, papermaking, and environmental protection fields, and also shows broad application prospects in biomedical materials such as wound dressings, artificial blood vessels, drug carriers, artificial cartilage, and bone tissue scaffolds. Currently known cellulose-producing bacteria include certain species from nine genera: *Acetobacter*, *Agrobacterium*, *Pseudomonas*, *Achromobacterium*, *Alcaligenes*, *Aerobiculbium*, *Azotobacter*, *Rhizobium*, and *Dystrophobicella*. Summary of the Invention

[0003] The purpose of this invention is to provide a method for preparing antibacterial bacterial cellulose, which uses simple raw materials to produce bacterial cellulose and ensures the redispersibility, structural stability and high quality of bacterial cellulose.

[0004] To achieve the above objectives, the present invention provides the following technical solution: A method for preparing antibacterial bacterial cellulose includes the following steps: Step 1: Prepare pure coconut water; Step 2: Boil the diluted coconut water solution to 100℃; Step 3: After the coconut water solution cools to 70-80℃, add an amino acid additive. Step 4: Maintain the temperature of the heated coconut water solution at 70-80℃ and add sodium hydroxide; Step 5: Filter the insulated coconut water solution, wash the filter residue thoroughly with distilled water until neutral, and dry to obtain the fiber sample.

[0005] Furthermore, the concentration of sodium hydroxide in step four is 2%.

[0006] Furthermore, the boiling time in step two is 95-100 minutes.

[0007] Furthermore, the amino acid additive in step three is one of lysine, tryptophan, and methionine.

[0008] Furthermore, the heating time in step three is 0.5-1 hour.

[0009] The beneficial effects of the present invention using the above structure are as follows: The method for preparing antibacterial bacterial cellulose proposed in this solution uses simple raw materials to produce bacterial cellulose, ensuring the redispersibility, structural stability and high quality of bacterial cellulose. Detailed Implementation

[0010] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention. Example

[0011] A method for preparing antibacterial bacterial cellulose includes the following steps: Step 1: Prepare pure coconut water; Step 2: Boil the diluted coconut water solution to 100℃; Step 3: After the coconut water solution cools to 70℃, add an amino acid additive. Step 4: Maintain the temperature of the heated coconut water solution at 70°C and add sodium hydroxide; Step 5: Filter the insulated coconut water solution, wash the filter residue thoroughly with distilled water until neutral, and dry to obtain the fiber sample.

[0012] The concentration of sodium hydroxide in step four is 2%.

[0013] The boiling time in step two is 95 minutes.

[0014] The amino acid additive in step three is one of lysine, tryptophan, and methionine.

[0015] The heating time in step three is 0.5 hours. Example

[0016] A method for preparing antibacterial bacterial cellulose includes the following steps: Step 1: Prepare pure coconut water; Step 2: Boil the diluted coconut water solution to 100℃; Step 3: After the coconut water solution cools to 75℃, add an amino acid additive. Step 4: Maintain the temperature of the heated coconut water solution at 75°C and add sodium hydroxide; Step 5: Filter the insulated coconut water solution, wash the filter residue thoroughly with distilled water until neutral, and dry to obtain the fiber sample.

[0017] The concentration of sodium hydroxide in step four is 2%.

[0018] The boiling time in step two is 98 minutes.

[0019] The amino acid additive in step three is one of lysine, tryptophan, and methionine.

[0020] The heating time in step three is 0.8 hours. Example

[0021] A method for preparing antibacterial bacterial cellulose includes the following steps: Step 1: Prepare pure coconut water; Step 2: Boil the diluted coconut water solution to 100℃; Step 3: After the coconut water solution cools to 80℃, add an amino acid additive. Step 4: Maintain the temperature of the heated coconut water solution at 80℃ and add sodium hydroxide; Step 5: Filter the insulated coconut water solution, wash the filter residue thoroughly with distilled water until neutral, and dry to obtain the fiber sample.

[0022] The concentration of sodium hydroxide in step four is 2%.

[0023] The boiling time in step two is 100 minutes.

[0024] The amino acid additive in step three is one of lysine, tryptophan, and methionine.

[0025] The heating time in step three is 1 hour.

[0026] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0027] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

[0028] The present invention and its embodiments have been described above, and such description is not restrictive. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the spirit of the invention, such designs should fall within the protection scope of the present invention.

Claims

1. A method for producing an antibacterial bacterial cellulose, characterized by, Includes the following steps: Step 1: Prepare pure coconut water; Step 2: Boil the diluted coconut water solution to 100℃; Step 3: After the coconut water solution cools to 70-80℃, add an amino acid additive. Step 4: Maintain the temperature of the heated coconut water solution at 70-80℃ and add sodium hydroxide; Step 5: Filter the insulated coconut water solution, wash the filter residue thoroughly with distilled water until neutral, and dry to obtain the fiber sample.

2. The method for preparing antibacterial bacterial cellulose according to claim 1, characterized in that: The concentration of sodium hydroxide in step four is 2%.

3. The method for preparing antibacterial bacterial cellulose according to claim 1, characterized in that: The boiling time in step two is 95-100 minutes.

4. The method for preparing antibacterial bacterial cellulose according to claim 1, characterized in that: The amino acid additive in step three is one of lysine, tryptophan, and methionine.

5. The method for preparing an antibacterial bacterial cellulose according to claim 1, characterized in that: The heating time in step three is 0.5-1 hour.