Preparation method and application of an acidic soil remediation bacterial agent
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHANDONG AGRI UNIV FERTILIZER TECH CO LTD
- Filing Date
- 2026-03-31
- Publication Date
- 2026-06-26
AI Technical Summary
Existing technologies are insufficient to effectively improve acidic soils to suit peach tree cultivation, resulting in poor growth and low yields in acidic soils.
An acid soil remediation agent was prepared by using a mixed bacterial powder containing Bacillus aureus and Bacillus licheniformis through centrifugation, spray drying, mixing, granulation and sieving processes. This agent can improve soil pH and nutrient content, improve soil structure and reduce diseases.
It significantly increased the pH value of acidic soil, reduced soil electrical conductivity, decreased the incidence of peach tree diseases, and increased yield per acre.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of soil remediation technology, specifically to a method for preparing and applying an acidic soil remediation microbial agent. Background Technology
[0002] Acidic soil is a general term for soils with a pH value less than 7. It includes lateritic red soil, red soil, yellow soil, and dry red soil, among others. Various types of red or yellow acidic soils are widely distributed in the tropical and subtropical regions of my country. These areas have high temperatures and abundant rainfall, with annual precipitation often exceeding 1500 mm. This combination of high temperature and high rainfall, along with the simultaneous occurrence of heat and humidity, results in intense soil weathering and soil formation, and a rapid cycle of biological matter. Simultaneously, there is a significant accumulation of iron and aluminum oxides, leading to acidic and infertile soil. The acidic red soils of the southeastern hilly regions are suitable for planting acidic crops such as tea trees.
[0003] Peach( Prunus persica *Prunus* (L.) Batsch, a deciduous small tree belonging to the genus *Prunus* in the family Rosaceae; the drupe is broadly ovoid-globose, densely covered with short, soft hairs; the pit is hard and woody; the seed is flattened-ovoid-cordate; the fruit ripening period varies depending on the variety, usually from August to September. The peach originated in China and has a cultivation history of over 4000 years, cultivated in Hebei, Shandong, Jiangsu, Zhejiang, and other regions. Peaches prefer sunlight and are intolerant of shade, thriving in mild climates; they are cold-hardy and drought-tolerant, but intolerant of waterlogging; they are best suited for planting in deep, humus-rich, well-drained, loose, fertile soils with strong water and fertilizer retention capabilities, and mostly grow on sunny or semi-sunny slopes with good sunlight. Peaches have poor tolerance to acidic soils; if the soil is acidic, it needs to be improved to meet the planting requirements of peaches. Therefore, the preparation of an acid-soil remediation microbial agent is of practical significance for the healthy growth and yield increase of peaches. Summary of the Invention
[0004] In view of the above-mentioned prior art, the purpose of this invention is to provide a method for preparing and applying an acidic soil remediation microbial agent.
[0005] To achieve the above objectives, the present invention adopts the following technical solution: In a first aspect, the present invention provides an acidic soil remediation microbial agent, comprising the following raw materials in parts by weight: 5-15 parts soil remediation microbial powder, 10-20 parts potassium feldspar powder, 20-35 parts dolomite powder, 20-30 parts activated humic acid, and 20-30 parts soybean meal powder. The soil remediation bacterial powder includes at least one of Bacillus argentisi powder or Bacillus licheniformis powder.
[0006] Furthermore, the number of effective live bacteria in the soil remediation bacterial powder is not less than 10. 8 CFU / g.
[0007] Furthermore, in the soil remediation bacterial powder, the ratio of viable bacteria of Bacillus aureus and Bacillus licheniformis is (1-3):(1-3).
[0008] A second aspect of the present invention provides a method for preparing the aforementioned acidic soil remediation microbial agent, comprising the following steps: (1) Centrifugation and spray drying: Soil remediation bacterial powder is obtained by centrifugation and spray drying in sequence; (2) Mixing: Mix the soil remediation bacteria powder, potassium feldspar powder, dolomite powder, activated humic acid, and soybean meal powder according to the required proportions. (3) Granulation: The material that has been mixed evenly is granulated after being quantitatively measured; (4) Screening: The granulated product is screened to obtain the acid soil remediation microbial agent.
[0009] Furthermore, the sieve mesh size is 200-600 mesh.
[0010] A third aspect of the invention provides the use of the acid soil remediation microbial agent in any of the following (i)-(iV): (i) Reduce soil EC value; (ii) Increase soil pH; (iii) Reduce the incidence of disease in peach trees; (iV) Increase the yield of peach trees per mu.
[0011] The beneficial effects of this invention are: This invention mixes *Bacillus aspergillus* powder and *Bacillus licheniformis* powder in a specific ratio to obtain a soil remediation bacterial powder, and further prepares an acidic soil remediation agent. When applied to peach tree cultivation, this agent reduces soil EC value, increases pH value, decreases peach tree disease incidence, and increases yield per acre. The example group showed the best performance, with an EC value of 0.37 mS / cm, a pH value of 6.73, a disease incidence rate of 5.2%, and a yield of 3010 kg per acre. It is evident that adding soil remediation bacterial powder containing live bacteria powder of *Bacillus aspergillus* and *Bacillus licheniformis* can significantly improve acidic soil, reduce disease incidence, and increase yield, better meeting the requirements for peach cultivation. Detailed Implementation
[0012] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.
[0013] To enable those skilled in the art to better understand the technical solution of this application, the technical solution of this application will be described in detail below with reference to specific embodiments.
[0014] Extracellular polysaccharides (EPS) are high-molecular-weight carbohydrates that microorganisms actively synthesize and secrete outside their cells during growth, metabolism, and adaptation to soil environments (drought, salinity, heavy metal stress). EPS achieves the synergistic restoration of soil physical, chemical, and biological properties by cementing soil, fixing heavy metals, degrading pollutants, and retaining water and fertilizer. This is the key mechanism by which microbial agents exert their effects.
[0015] Bacillus argentea ( Bacillus aryabhattai The main secretions of this plant are alkali, extracellular polysaccharides (EPS), phosphorus and potassium solubilizing enzymes, nitrogenase, IAA, gibberellins, and other growth-promoting substances. Its effects on acidic soil remediation are as follows: 1. Stabilizing soil pH; 2. Strongly activating acidic soil by fixing nutrients, secreting gluconic acid and citric acid, chelating iron, aluminum, and calcium, and releasing phosphorus, potassium, zinc, and magnesium, thus solving the "phosphorus deficiency" problem in acidic soils; 3. Retaining water and fertilizer, reducing nutrient leaching: Acidic soils experience heavy rainfall and nutrient loss; EPS adsorbs nutrients, increasing fertilizer retention by over 30%; 4. Alleviating heavy metal toxicity (acidic soils have high cadmium and lead activity): EPS chelates heavy metals, reducing their availability and decreasing crop absorption; 5. Promoting root development and improving acid resistance: Secreting IAA stimulates root growth and enhances crop survival rates in acidic environments.
[0016] Bacillus licheniformis ( Bacillus licheniformis The main secretions are ammonia, urease, extracellular polysaccharides (EPS), protease, phosphate solubilizer, nitrogen-fixing related enzymes, and antibacterial lipopeptides. Its effects on acidic soil remediation are as follows: 1. Increase soil pH and neutralize acidity: Secreting ammonia and urease decomposes soil nitrogen to produce alkaline substances, alleviating aluminum and hydrogen toxicity. 2. Passivate active aluminum and reduce acid damage: Secretions complex with active Al³⁺ in the soil, reducing aluminum toxicity to roots and promoting root growth. 3. Improve acidic soil structure: Acidic soils are prone to compaction and heavy clay formation; EPS binds soil particles to form aggregates, increasing aeration and water permeability. 4. Solubilize phosphorus and release potassium, improving nutrient availability. 5. Inhibit soil-borne diseases and improve the microecology: Secreting lipopeptides inhibits common diseases in acidic soils such as Fusarium and root rot, reducing continuous cropping obstacles.
[0017] The experimental materials used in the embodiments of this invention, unless otherwise specified, are all conventional experimental materials in the art and can be purchased through commercial channels. The Bacillus aureus used is... Bacillus aryabhattai The information has been disclosed in "CN113755382B A strain of Bacillus licheniformis NDFY-1 and its application"; the Bacillus licheniformis used ( Bacillus licheniformisThe following materials were purchased from the China General Microbiological Culture Collection Center (CGMCC), CGMCC number CGMCC 1.64714: potassium feldspar powder with SiO2 ≥ 35% was purchased from Shandong Youquan Science and Trade Co., Ltd.; dolomite powder with CaO ≥ 25% and MgO ≥ 18% was purchased from Junan Shimeng Mining Co., Ltd.; activated humic acid with organic matter ≥ 50% was purchased from Shandong Agricultural University Fertilizer Technology Co., Ltd.; and soybean meal powder with organic matter ≥ 60% was purchased from Shangqiu Tianchen Biotechnology Co., Ltd.
[0018] Example 1: Acidic Soil Remediation Microbial Agent The composition of the acid soil remediation microbial agent is as follows: 10wt% soil remediation microbial powder, 15wt% potassium feldspar powder, 28wt% dolomite powder, 25wt% activated humic acid, and 22wt% soybean meal powder.
[0019] The soil remediation bacterial powder used consists of Bacillus aspergillus powder and Bacillus licheniformis powder, with an effective viable bacteria count of 10. 9 The CFU / g ratio of viable Bacillus aureus to Bacillus licheniformis was 2:3.
[0020] The preparation method of acid soil remediation microbial agent is as follows: (1) Centrifugation and spray drying: Soil remediation bacterial powder is produced using centrifuges and spray drying equipment; (2) Mixing: Soil remediation bacterial powder, potassium feldspar powder, dolomite powder, activated humic acid, and soybean meal powder are added to the mixing tank according to the required proportions using a quantitative scale and then mixed. (3) Granulation: The uniformly mixed material is fed into the extrusion granulator through a quantitative weighing scale for granulation; (4) Screening: The granulated product is screened through a 400-mesh sieve to finally obtain the acid soil remediation microbial agent.
[0021] The extracellular polysaccharide content in the acid soil remediation microbial agent was determined to be 1.3 mg / g.
[0022] Example 2: Acidic Soil Remediation Microbial Agent The composition of the acid soil remediation microbial agent is as follows: 10wt% soil remediation microbial powder, 15wt% potassium feldspar powder, 28wt% dolomite powder, 25wt% activated humic acid, and 22wt% soybean meal powder.
[0023] The soil remediation bacterial powder used consists of Bacillus aspergillus powder and Bacillus licheniformis powder, with an effective viable bacteria count of 10. 9 The CFU / g ratio of viable Bacillus aureus to Bacillus licheniformis was 3:2.
[0024] The preparation method of acid soil remediation microbial agent is as follows: (1) Centrifugation and spray drying: Soil remediation bacterial powder is produced using centrifuges and spray drying equipment; (2) Mixing: Soil remediation bacterial powder, potassium feldspar powder, dolomite powder, activated humic acid, and soybean meal powder are added to the mixing tank according to the required proportions using a quantitative scale and then mixed. (3) Granulation: The uniformly mixed material is fed into the extrusion granulator through a quantitative weighing scale for granulation; (4) Screening: The granulated product is screened through a 400-mesh sieve to finally obtain the acid soil remediation microbial agent.
[0025] The extracellular polysaccharide content in the acid soil remediation microbial agent was determined to be 1.1 mg / g.
[0026] Example 3: Acidic Soil Remediation Microbial Agent The composition of the acid soil remediation microbial agent is as follows: 10wt% soil remediation microbial powder, 15wt% potassium feldspar powder, 28wt% dolomite powder, 25wt% activated humic acid, and 22wt% soybean meal powder.
[0027] The soil remediation bacterial powder used consists of Bacillus aspergillus powder and Bacillus licheniformis powder, with an effective viable bacteria count of 10. 9 The CFU / g ratio of viable Bacillus aureus to Bacillus licheniformis was 1:1.
[0028] The preparation method of acid soil remediation microbial agent is as follows: (1) Centrifugation and spray drying: Soil remediation bacterial powder is produced using centrifuges and spray drying equipment; (2) Mixing: Soil remediation bacterial powder, potassium feldspar powder, dolomite powder, activated humic acid, and soybean meal powder are added to the mixing tank according to the required proportions using a quantitative scale and then mixed. (3) Granulation: The uniformly mixed material is fed into the extrusion granulator through a quantitative weighing scale for granulation; (4) Screening: The granulated product is screened through a 400-mesh sieve to finally obtain the acid soil remediation microbial agent.
[0029] The extracellular polysaccharide content in the acid soil remediation microbial agent was determined to be 1.2 mg / g.
[0030] Comparative Example 1 The difference between this comparative example and Example 1 is that the soil remediation bacterial powder used is Bacillus argentisi powder.
[0031] Comparative Example 2 The difference between this comparative example and Example 1 is that the soil remediation bacterial powder used is Bacillus licheniformis bacterial powder.
[0032] Comparative Example 3 The difference between this comparative example and Example 1 is that the soil remediation bacterial powder used is Bacillus aspergillus powder and Bacillus licheniformis powder, but both have been inactivated.
[0033] Test case To verify the efficacy of the soil remediation microbial agent of this invention, a field experiment was conducted in Bianguanzhuang Village, Zhaizili Town, Laiwu City, Jinan, Shandong Province. The experimental site was flat with good irrigation and drainage conditions. The tested soil was brown soil with moderate and uniform fertility. The tested peach tree variety was Hongguan, five years old, planted in holes at a rate of 57 trees per mu (approximately 0.067 hectares), and covered with straw.
[0034] This experiment consisted of four experimental groups and one control group, arranged in a randomized block design with three replicates. Each plot contained six plants, and the protection row was 1 meter long. The groupings are as follows: Experimental group: In addition to the conventional basal application of 54 kg of conventional compound fertilizer (with nitrogen, phosphorus and potassium contents of 16%, 10% and 19% respectively), 39.9 kg of "soil remediation microbial agent" was applied per mu (unit of land area). Among them, the soil remediation microbial agents used in the example group and comparative examples 1-3 were from example 1 and comparative examples 1-3, respectively.
[0035] CK group: blank treatment, no fertilizer applied.
[0036] Soil samples were tested prior to the experiment, including EC and pH values. The EC value was 0.39 mS / cm, and the pH value was 6.40. The experiment lasted from April to August, and field management was as follows: During the peach tree growing season, 15g of 10% imidacloprid was diluted in 40kg of water per mu (approximately 0.067 hectares) for pest control. Other management measures were the same as in conventional field operations. The results are shown in Table 1.
[0037] Table 1 Test Results Table 1 shows that compared with the control group (CK), the experimental groups exhibited lower EC values, higher pH values, lower disease incidence, and higher yield per mu (667 square meters). The example group showed the best performance, with an EC value of 0.37 mS / cm, a pH value of 6.73, a disease incidence of 5.2%, and a yield of 3010 kg per mu. This demonstrates that adding soil remediation bacterial powder containing live bacteria powder of Bacillus aspergillus and Bacillus licheniformis can significantly improve acidic soil, reduce disease incidence, and increase yield, thus better meeting the requirements for peach cultivation.
[0038] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.
Claims
1. An acidic soil remediation bacterial agent, characterized by, The raw materials include the following parts by weight: 5-15 parts soil remediation bacterial powder, 10-20 parts potassium feldspar powder, 20-35 parts dolomite powder, 20-30 parts activated humic acid, and 20-30 parts soybean meal powder. The soil remediation bacterial powder includes at least one of Bacillus argentisi powder or Bacillus licheniformis powder.
2. The acidic soil remediation microbial agent according to claim 1, characterized in that, The effective live bacteria count in the soil remediation bacterial powder is not less than 10. 8 CFU / g.
3. The acidic soil remediation microbial agent according to claim 1, characterized in that, In the soil remediation bacterial powder, the ratio of viable bacteria of Bacillus aureus and Bacillus licheniformis is (1-3):(1-3).
4. The method for preparing the acidic soil remediation microbial agent according to any one of claims 1-3, characterized in that, Includes the following steps: (1) Centrifugation and spray drying: Soil remediation bacterial powder is obtained by centrifugation and spray drying in sequence; (2) Mixing: Mix the soil remediation bacteria powder, potassium feldspar powder, dolomite powder, activated humic acid, and soybean meal powder according to the required proportions. (3) Granulation: The material that has been mixed evenly is granulated after being quantitatively measured; (4) Screening: The granulated product is screened to obtain the acid soil remediation microbial agent.
5. The method for preparing the acidic soil remediation microbial agent according to claim 4, characterized in that, The sieve mesh size is 200-600 mesh.
6. The use of the acid soil remediation microbial agent according to any one of claims 1-3 in any one of the following (i)-(iV): (i) Reduce soil EC value; (ii) Increase soil pH; (iii) Reduce the incidence of disease in peach trees; (iV) Increase the yield of peach trees per mu.