Establishment method and application of mouse gastric squamous carcinoma cell line and gastric cancer related fibroblast line

By establishing genetically stable mouse gastric squamous cell carcinoma cell lines and fibroblast cell lines, the problem of inaccurate models in existing technologies has been solved, enabling the construction of tumor microenvironment models in immune-active mice, supporting immunotherapy research and drug evaluation.

CN122278748APending Publication Date: 2026-06-26BEIJING CANCER HOSPITAL PEKING UNIV CANCER HOSPITAL
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Patent Information

Application Number
CN202610397620.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-30
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

The lack of genetically stable mouse gastric squamous cell carcinoma cell lines and related fibroblast cell lines in current technologies makes it difficult to construct accurate tumor microenvironment models, affecting drug treatment evaluation and immunotherapy research.

Method used

Using a specific culture medium and method, mouse gastric squamous cell carcinoma cell lines and gastric cancer-associated fibroblast cell lines were isolated and cultured from spontaneous liver metastasis tumor tissues in mice. Genetically stable cell lines were established using L-WRN cell culture supernatant, Matrigel, and other components combined with flow cytometry sorting technology.

Benefits of technology

It provides genetically stable mouse gastric squamous cell carcinoma cell lines and fibroblast cell lines, which can be used to construct ectopic or in situ implantation models in immune-active mice for studying the gastric cancer tumor microenvironment, including exploring biomarkers for immunotherapy efficacy and evaluating new drugs.

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Abstract

The invention provides an establishment method and application of a mouse gastric squamous carcinoma cell line and a gastric cancer related fibroblast line, and a culture medium for culturing the mouse gastric squamous carcinoma cell line and the gastric cancer related fibroblast line comprises L-WRN cell culture supernatant, and 0.5 vol% to 1.5 vol% of a 100 * N-2 additive, 1.5 vol% to 2.5 vol% of a 50 * B-27 additive containing no vitamin A, 1 mM to 1.5 mM of N-acetylcysteine amide, 8 mM to 12 mM of niacinamide, 80 ng / mL to 120 ng / mL of FGF-10, 40 ng / mL to 60 ng / mL of EGF, 0.8 [mu] M to 1.2 [mu] M of A83-01, 8 [mu] M to 12 [mu] M of Y-27632 dihydrochloride, 0.5 vol% to 1.5 vol% of a Glutamax additive, 5 vol% to 7 vol% of Matrigel, 8 mM to 12 mM of a Hepes buffer solution and 0.8 nM to 1.2 nM of gastrin. The culture medium provided by the invention can greatly improve the in-vitro culture success rate of a primary cell line, and the cultured mouse gastric squamous carcinoma cell line and gastric cancer related fibroblast line are stable in heredity and can be applied to construction of an ectopic or in-situ planting model in immunocompetence mice.
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