SNP combination for paternity test of cynomolgus monkeys and paternity test method

By combining SNP loci specific to cynomolgus monkeys and using low-depth sequencing methods, the problems of high accuracy and low cost in cynomolgus monkey parentage testing have been solved, enabling high-throughput, low-cost parentage testing and meeting the needs of large-scale management of experimental monkey populations.

CN122279062AActive Publication Date: 2026-06-26ACADEMY OF MILITARY MEDICAL SCIENCES
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Patent Information

Application Number
CN202610747886.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-28
Publication Date
2026-06-26
Estimated Expiration
2046-05-28

AI Technical Summary

Technical Problem

Existing technologies are insufficient to meet the requirements of high precision, low cost, and high throughput for cynomolgus monkey parentage testing. STR markers are prone to typing discrepancies, and SNP markers lack species specificity and have high testing costs, which cannot meet the needs of large-scale management of experimental monkey populations.

Method used

This invention provides a specific SNP locus combination (SNP1~SNP100) for cynomolgus monkeys, which is combined with low-depth sequencing for paternity testing, including DNA extraction, SNP typing and data analysis, to establish clear criteria for determining parentage and reduce testing costs and technical barriers.

Benefits of technology

It achieves high-precision, low-cost paternity testing for cynomolgus monkeys, reducing the cost of single-sample testing to 1/5 to 1/10 of existing high-depth sequencing. It is suitable for pedigree management and genetic quality control of large-scale experimental monkey populations, improving testing efficiency and result consistency.

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Abstract

The invention relates to the technical field of molecular marker screening, in particular to an SNP (Single Nucleotide Polymorphism) combination for macaca fascicularis paternity test and a paternity test method. According to the identification method provided by the invention, 100 screened special SNP combinations (SNP1-SNP100) are combined with low-depth sequencing to carry out macaca fascicularis paternity identification, the detection cost of a single sample is only 1 / 5-1 / 10 of that of the existing high-depth sequencing and 1 / 2 of that of an SNP chip, hundreds of thousands of yuan of detection cost can be saved every year for large-scale experimental macaca populations (such as thousands of populations), and the detection efficiency is greatly improved. The economic burden of breeding and management of experimental monkeys is greatly reduced, and the method has extremely high economical efficiency. The identification method provided by the invention is suitable for population pedigree management, genetic relationship tracing, breeding pairing optimization and genetic quality control of cynomolgus monkeys, and can be widely applied to experimental monkey breeding fields, primate animal research centers, biological medicine research and development institutions and other scenes.
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