Intelligent biochip detection all-in-one machine

Abstract

This invention discloses an intelligent biochip detection all-in-one machine, relating to the field of biochip detection technology. It includes: a base structure, a worktable structure fixed to the top of the base structure, and a displacement structure fixed to the top of the entire machine. The displacement structure includes a gantry frame and a mounting frame, with the mounting frame slidably connected to the gantry frame, allowing for full-range movement on the top plane of the worktable structure. A photographic interpretation structure for capturing chip detection results is mounted on the mounting frame. This invention, through a three-axis linkage architecture of the gantry frame and mounting frame of the displacement structure, combined with a separate drive design using four sets of independent vertical lead screws, achieves independent vertical lifting and full-range planar movement of the photographic interpretation structure, gripper module, waste liquid absorption module, and pipetting module. This solves the problems of mutual interference between the moving parts, low drive accuracy, and insufficient automation in traditional detection equipment.

Description

Technical Field

[0001] This invention relates to the field of biochip detection technology, specifically to an integrated intelligent biochip detection machine. Background Technology

[0002] Intelligent biochips are core detection carriers integrating microarray technology and biosensing technology. By immobilizing specific probes on the chip surface, they can undergo specific hybridization reactions with target nucleic acids (DNA / RNA) in the sample, thereby achieving qualitative detection of targets such as pathogens and gene loci. They are widely used in clinical diagnosis, livestock disease monitoring, food safety testing, and many other fields. A typical detection process involves: first, sample pretreatment and nucleic acid amplification; then, the amplification products are loaded into specific wells on the biochip; subsequently, the chip undergoes a series of reaction steps including hybridization, washing, incubation, and color development to visualize the binding signal between the target nucleic acid and the probe; finally, the signal is analyzed by an imaging and interpretation system to output the detection results.

[0003] However, existing intelligent biochip detection devices still face two major technical challenges in practical applications: Poor coordination between equipment drive and automation: Existing equipment often uses a single drive mechanism to control multiple actuators, resulting in mutual interference between actions such as needle picking, tray handling, liquid pipetting, and needle retrieval, making it difficult to guarantee drive accuracy; moreover, the functional modules are scattered and lack integrated design, making it impossible to achieve fully automated closed-loop operation. Manual intervention is required in intermediate steps (such as manually adjusting the tray position and replacing the needle), which not only reduces detection efficiency but also increases the risk of human error and sample contamination, making it difficult to meet the actual needs of batch sample testing; The reliability of carrier plate and chip handling is insufficient: Traditional detection equipment often uses rigid metal grippers for direct contact with the carrier plate. The impact force during gripping can easily scratch the carrier plate surface or damage the chip, leading to sample contamination or signal distortion. Simultaneously, the friction between the grippers and the carrier plate is insufficient, making it prone to slippage during handling. Furthermore, the lack of a precise positioning structure results in carrier plate displacement after gripping, directly affecting the accuracy of subsequent pipetting and ultimately increasing the error in the detection results. Therefore, this paper proposes an intelligent biochip detection integrated machine to address these issues. Summary of the Invention

[0004] Technical problems to be solved To address the shortcomings of existing technologies, this invention provides an integrated intelligent biochip detection machine, which solves the problems mentioned in the background section.

[0005] Technical solution To achieve the above objectives, the present invention is implemented through the following technical solution: an intelligent biochip detection integrated machine, comprising a base structure, a worktable structure fixed to the top of the base structure, and a displacement structure fixed to the top of the whole machine; The displacement structure includes a gantry frame and a mounting frame. The mounting frame is slidably connected to the gantry frame and can move in its entire range on the top plane of the workbench structure. The mounting frame is equipped with an image interpretation structure for capturing chip detection results, a gripper module for chip transfer, a waste liquid absorption module for waste liquid extraction, and a pipetting module for reagent dispensing. The image interpretation structure, gripper module, waste liquid absorption module, and pipetting module can all move in a plane synchronously with the mounting frame, and can all independently complete vertical lifting and lowering movements via a lead screw. The image interpretation structure includes a light source and an industrial camera. The image interpretation structure can be driven to rise and fall vertically by a lead screw. The light source and the industrial camera are arranged coaxially, and the illumination direction of the light source is consistent with the focusing direction of the lens of the industrial camera. The bottom of the gripper module is provided with two sets of clamps that can open and close synchronously in opposite directions and are arranged opposite each other. The base structure is equipped with a sealed collection chamber for collecting waste consumables and experimental waste liquid. The workbench structure is fixed with a vertically connected collection frame, the bottom of which is connected to the top channel of the sealed collection chamber.

[0006] Preferably, the gantry frame is provided with left-right extending X-axis linear slide rails, and a transverse beam is slidably connected to the X-axis linear slide rails via sliding blocks. A front-back extending Y-axis linear slide rail is fixed on the transverse beam, and the transverse beam can drive the Y-axis linear slide rail to complete left-right displacement along the X-axis linear slide rail. The mounting frame is connected to the Y-axis linear slide rail via sliding blocks and can complete front-back longitudinal displacement along the Y-axis linear slide rail.

[0007] Preferably, the mounting frame is provided with four sets of independent vertical drive screws: a first screw, a second screw, a third screw, and a fourth screw; the image interpretation structure is connected to the first screw via a sliding block, the gripper module is connected to the second screw via a sliding block, the waste liquid absorption module is connected to the third screw via a sliding block, and the pipetting module is connected to the fourth screw via a sliding block.

[0008] Preferably, the image interpretation structure is installed on the outer end of the first side of the mounting frame along the Y-axis direction, and the gripper module is installed on the inner end of the same side of the mounting frame; the pipetting module is installed on the outer end of the second side of the mounting frame along the length direction, and the waste liquid absorption module is installed on the inner end of the same side of the mounting frame.

[0009] Preferably, the gripper module includes a mounting base connected to a second lead screw via a sliding block, a drive motor fixedly mounted at the bottom of the mounting base, and a track seat fixedly mounted at the output end of the drive motor; the bottom of the track seat has a laterally extending sliding groove, and two sets of synchronously opposite sliding seats are slidably connected in the sliding groove, with a clamping plate fixedly mounted at the bottom of each set of sliding seats.

[0010] Preferably, the inner wall of the clamping plate is fixedly provided with a first protrusion and a rubber strip; the rubber strip is fixedly provided with a second protrusion; the first protrusion is a rigid positioning structure, symmetrically distributed on both sides of the inner wall of the clamping plate; the second protrusion is an arc-shaped rubber protrusion, evenly arranged along the length direction of the rubber strip.

[0011] Preferably, the pipetting module includes a pipette connected to a fourth lead screw via a sliding block, a sample needle adapter fixedly mounted at the bottom of the pipette, and a disposable sample needle that can be assembled on the sample needle adapter by interference fit; a push block is fixedly mounted on the sample needle adapter, and the push block is coaxially arranged with the sample needle for pushing the sample needle down from the sample needle adapter.

[0012] Preferably, the waste liquid absorption module includes a suction seat connected to a third lead screw via a sliding block. A suction needle is fixedly provided at the bottom of the suction seat, and a suction interface is fixedly provided on the side wall. The suction interface is connected to the internal flow channel of the suction needle. A negative pressure pump for providing negative pressure power for waste liquid extraction is fixedly provided on the inner side wall of the whole machine. The inlet end of the negative pressure pump is connected to the suction interface via a delivery pipe, and the outlet end is connected to the sealed collection cavity of the base structure via a delivery pipe.

[0013] Preferably, the workbench structure includes a first workbench and a second workbench fixed side by side. The first workbench is equipped with an adjustable frequency oscillation component and a heating module, and the second workbench has a built-in temperature control module for heating and cooling. A liftable heat cover is fixed above the first workbench for heating the cover on the chip. The left side of the workbench structure is equipped with a chip compartment, and the right side is equipped with a reagent consumable compartment. Both the chip compartment and the reagent consumable compartment are slidably connected to the workbench structure via electric guide rails.

[0014] Preferably, the base structure includes a cabinet, and the sealed collection chamber is located inside the cabinet; the sealed collection chamber includes an independent sample needle collection box and a waste liquid collection box, both of which are installed in the cabinet cavity via an electric sliding guide rail; a liquid level sensor is fixed to the outer wall of the waste liquid collection box, and the cabinet cavity is also equipped with a sterilization component and a heat dissipation component.

[0015] Beneficial effects The present invention has the following beneficial effects: (1) This intelligent biochip detection all-in-one machine, through the three-axis linkage architecture of the gantry shaft frame and the mounting frame with the displacement structure, and the split drive design with four sets of independent vertical screws, realizes the independent vertical lifting and full-range planar movement of the photo reading structure, gripper module, waste liquid absorption module and pipetting module, which solves the problems of mutual interference of the motion of the execution parts of traditional detection equipment, low driving accuracy and insufficient automation.

[0016] (2) The intelligent biochip detection integrated machine solves the problems of poor stability, easy slippage and damage, and low positioning accuracy of traditional chip transfer and clamping by setting a first protrusion, a rubber strip and a second protrusion on the inner wall of the clamping plate of the gripper module, and cooperating with the synchronous reverse precise sliding structure of the slide and the track seat.

[0017] (3) The intelligent biochip detection all-in-one machine, through the dual-station split design of the workbench structure, integrates adjustable frequency oscillation and heating functions on the first workbench and high-precision heating and cooling functions on the second workbench, which solves the problem that traditional equipment has single functions and cannot adapt to the detection needs of multiple types of biochips.

[0018] (4) The intelligent biochip detection integrated machine, through the structural design of independent installation and physical separation of the liquid transfer module and the waste liquid absorption module, combined with the collection frame that runs through the top and bottom and the independent sealed collection chamber in the base, solves the problems of high risk of cross-contamination and insufficient biosafety protection of traditional equipment.

[0019] Of course, any product implementing this invention does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of the intelligent biochip detection integrated machine of the present invention; Figure 2 This is a schematic diagram of the base structure in the intelligent biochip detection integrated machine of the present invention; Figure 3 This is a schematic diagram of the workbench structure in the intelligent biochip detection integrated machine of the present invention; Figure 4 This is a schematic diagram of the displacement structure in the intelligent biochip detection integrated machine of the present invention; Figure 5 This is a partial structural diagram of the intelligent biochip detection integrated machine of the present invention; Figure 6 This is a schematic diagram of the image interpretation module in the intelligent biochip detection all-in-one machine of the present invention; Figure 7 This is a schematic diagram of the gripper module in the intelligent biochip detection integrated machine of the present invention; Figure 8This is a partial structural diagram of the gripper module in the intelligent biochip detection integrated machine of the present invention; Figure 9 This is a schematic diagram of the clamping plate in the intelligent biochip detection integrated machine of the present invention; Figure 10 This is a partial structural diagram of the waste liquid absorption module, the liquid transfer module, and the mounting frame in the intelligent biochip detection integrated machine of the present invention.

[0021] In the diagram: 1. Base structure; 101. Base cabinet; 102. Sample needle collection box; 103. Waste liquid collection box; 2. Workbench structure; 201. Chip compartment; 202. First workbench; 203. Heated cover; 204. Second workbench; 205. Reagent and consumable compartment; 3. Displacement structure; 301. Gantry shaft frame; 302. Mounting frame; 4. Photo interpretation structure; 401. First lead screw; 402. Industrial camera; 403. Light source; 5. Gripper module; 50 1. Second lead screw; 502. Drive motor; 503. Track seat; 504. Slide seat; 505. Clamping plate; 506. First protrusion; 507. Rubber strip; 508. Second protrusion; 6. Waste liquid absorption module; 601. Third lead screw; 602. Liquid suction seat; 603. Liquid suction interface; 604. Liquid suction needle; 7. Pipetting module; 701. Fourth lead screw; 702. Pipettor; 703. Sample needle adapter; 704. Sample dispensing needle; 705. Push block. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] The intelligent biochip detection integrated machine of this embodiment is divided into three layers according to the spatial layout: bottom support layer, middle functional layer and top transmission layer. The core includes base structure 1, workbench structure 2, displacement structure 3, and four major execution modules arranged on displacement structure 3: image interpretation structure 4, gripper module 5, waste liquid absorption module 6, and liquid transfer module 7.

[0024] The base structure 1 is the main support body at the bottom of the whole machine, including the base cabinet 101, the sample needle collection box 102, and the waste liquid collection box 103; The base cabinet 101 is equipped with adjustable shock-absorbing support feet at the four corners of its bottom, which can be adapted to different installation surfaces and offset the vibration generated during equipment operation. Two sets of electric sliding guide rails are arranged in parallel in the bottom cavity of the cabinet 101. The sample needle collection box 102 and the waste liquid collection box 103 are slidably connected to the two sets of electric sliding guide rails through sliding blocks. The two are independent sealed boxes that can be automatically slid out of the cabinet 101 along the guide rails by electric drive, which makes it convenient for operators to centrally handle waste consumables and experimental waste liquids. The waste liquid collection box 103 is equipped with a liquid level sensor on its outer wall, which can monitor the liquid level of the waste liquid in real time. When the liquid level reaches the warning threshold, it can send a trigger signal to the equipment control system. Two exhaust fans are symmetrically fixed to the inner side wall of the cabinet 101. The air intake of the exhaust fans faces the communication area between the inner cavity of the cabinet 101 and the detection chamber in the middle of the equipment. An ultraviolet sterilization lamp is fixed at a distance of 50-100mm between the air intake and the communication area, which can realize continuous sterilization and ventilation of the inner cavity of the equipment.

[0025] The workbench structure 2 is fixed on the top of the base structure 1 and serves as the main functional support in the middle of the machine. It includes a chip compartment 201, a first workbench 202, a hot cover 203, a second workbench 204, and a reagent consumable compartment 205. The chip compartment 201 is installed on the left side of the workbench structure 2 via an electric guide rail. The compartment body cooperates with the electric guide rail via a sliding block and can be automatically moved in and out of the compartment by electric drive. It is used for loading the chips to be tested and recycling and storing the chips after testing. The reagent consumables compartment 205 is installed on the right side of the workbench structure 2 via an electric guide rail. The compartment body cooperates with the electric guide rail via a sliding block, and can be automatically moved in and out of the compartment by electric drive. The reagent consumables compartment 205 integrates a sample needle storage area and a reagent storage area, which are used to store disposable plastic sample needles 704 and test reagents, respectively; the first workbench 202 and the second workbench 204 are fixedly arranged side by side in the middle area of ​​the workbench structure 2, and both of them have chip positioning slots on their upper surfaces, which can realize precise positioning and fixing of the chip. The first workbench 202 is internally embedded with an adjustable frequency oscillation component and a heating module, which can simultaneously realize heating and shaking to meet the mixing requirements of chip hybridization and color development reactions; the second workbench 204 is a high-precision heating and cooling module, which can realize heating and cooling within the range of 30℃~60℃, with a temperature control accuracy of ≤±0.3℃, to meet the reaction requirements of precise temperature control for chip incubation. The heated cover 203 is an electrically driven lifting structure, fixed directly above the first worktable 202. It can be lowered and closed to heat the cover on the chip, preventing reagent evaporation during the reaction process from affecting the detection results. A collection frame is fixed between the first workbench 202 and the reagent consumables compartment 205. The collection frame is a fully enclosed guide cavity that runs vertically through the top and bottom. Its bottom is connected to the top drop channel of the sample needle collection box 102, which is used for the directional guidance and recycling of the waste sample needle 704.

[0026] The displacement structure 3 is fixed on the top of the machine and is the main body of the three-axis linkage drive of the machine, including the gantry frame 301 and the mounting frame 302. The gantry frame 301 is a double gantry symmetrical structure, which is fastened to the top of the equipment frame with bolts. It consists of longitudinal columns fixed in parallel on both sides and transverse beams spanning between the two sets of columns. The two side columns of the gantry frame 301 are evenly provided with left and right extended X-axis linear slide rails. The two ends of the transverse beam are slidably connected to the X-axis linear slide rails through sliding blocks, and can complete left and right lateral displacement along the column. The transverse beam is provided with a Y-axis linear slide rail extending forward and backward. The mounting frame 302 is slidably connected to the Y-axis linear slide rail by a sliding block, and can complete the forward and backward longitudinal displacement along the transverse beam. By coordinating the left and right displacement of the transverse beam with the front and rear displacement of the mounting frame 302, the mounting frame 302 can achieve full-range coverage movement on the top plane of the workbench structure 2. The image interpretation structure 4, gripper module 5, waste liquid absorption module 6, and liquid transfer module 7 are all installed on the mounting frame 302 and can move synchronously across the entire plane with the mounting frame 302 to meet the operational needs of all workstations.

[0027] The image processing and interpretation structure 4 includes an industrial camera 402 and a light source 403. The image processing and interpretation structure 4 is fixedly mounted on a mounting frame 302 via a mounting base. A first lead screw 401 and a Z-axis slide rail are vertically and parallelly arranged on the mounting base. The image processing and interpretation structure is fixed to the transmission nut of the first lead screw 401 via a mounting seat, while the mounting seat is slidably connected to the Z-axis slide rail via a sliding block. The first lead screw 401 is driven by a servo motor, which can drive the image processing and interpretation structure to complete independent vertical lifting and lowering movements along the Z-axis slide rail, achieving precise adjustment of the image focusing distance. The illumination direction of the light source 403 is completely consistent with the focusing direction of the lens of the industrial camera 402, which can provide uniform and stable illumination for chip imaging and realize high-definition imaging of the chip reaction holes one by one.

[0028] The gripper module 5 is fixedly mounted on the mounting frame 302 via the second lead screw 501 and the Z-axis slide rail. The second lead screw 501 and the Z-axis slide rail are both arranged vertically and parallel to each other. The drive motor 502 is fixed to the transmission nut of the second lead screw 501 through the mounting base. At the same time, the mounting base is slidably connected to the Z-axis slide rail through the sliding block. The second lead screw 501 is driven by a servo motor, which can drive the drive motor 502 and all the components below it to complete independent vertical lifting and lowering movements along the Z-axis slide rail. The bottom output end of the drive motor 502 is fixed with a track seat 503. The bottom of the track seat 503 is provided with a horizontally extending slide groove. Two sets of synchronously reverse sliding slide seats 504 are slidably connected in the slide groove. The drive motor 502 can drive the two sets of slide seats 504 to complete synchronous reverse sliding along the slide groove. Each set of slides 504 has a clamping plate 505 fixed at the bottom. The two clamping plates 505 are set to cooperate with each other and open and close synchronously with the sliding of the slides 504 to complete the chip gripping and releasing action. The inner wall of the clamping plate 505 is fixed with a first protrusion 506 and a rubber strip 507, and a second protrusion 508 is fixed on the rubber strip 507; the first protrusion 506 is a rigid positioning structure, symmetrically distributed on both sides of the inner wall of the clamping plate 505, and is used to quickly calibrate the chip position during the clamping process. The rubber strip 507 is fully attached to the length of the clamping plate 505, which can buffer the impact force of clamping and avoid hard contact that could scratch the chip. The second protrusion 508 is an arc-shaped rubber protrusion, which is evenly distributed along the length of the rubber strip 507. It can increase the gripping friction and prevent the chip from slipping and falling during transportation.

[0029] The waste liquid absorption module 6 is fixedly installed on the mounting frame 302 via a third lead screw 601 and a Z-axis slide rail. The third lead screw 601 and the Z-axis slide rail are both arranged vertically and parallel to each other. The liquid suction seat 602 is fixed on the transmission nut of the third lead screw 601, and the liquid suction seat 602 is slidably connected to the Z-axis slide rail via a sliding block. The third lead screw 601 is driven by a servo motor, which can drive the liquid suction seat 602 to complete independent vertical lifting and lowering movements along the Z-axis slide rail. The bottom of the suction seat 602 is fixedly provided with a suction needle 604, and the side wall of the suction seat 602 is fixedly provided with a suction port 603, which is completely connected to the internal flow channel of the suction needle 604. A negative pressure pump is fixedly installed on the inner wall of the whole machine. The liquid inlet of the negative pressure pump is connected to the liquid suction interface 603 through the delivery pipe, and the liquid outlet is connected to the waste liquid collection box 103 through the delivery pipe. The negative pressure power realizes the extraction and directional delivery of waste liquid in the chip reaction hole.

[0030] The pipetting module 7 is fixedly mounted on the mounting frame 302 via the fourth lead screw 701 and the Z-axis slide rail; both the fourth lead screw 701 and the Z-axis slide rail are arranged vertically and parallel to each other, and the pipette 702 is fixed on the transmission nut of the fourth lead screw 701. At the same time, the pipette 702 is slidably connected to the Z-axis slide rail via a sliding block; the fourth lead screw 701 is driven by a servo motor, which can drive the pipette 702 to complete independent vertical lifting and lowering movements along the Z-axis slide rail; The pipette 702 has a closed structure and internally encloses a high-precision pipetting drive component. A sample needle adapter 703 is fixedly installed at the bottom of the pipette 702. A disposable sample needle 704 can be assembled on the sample needle adapter 703 through an interference fit. The sample needle 704 is automatically assembled by the downward pressure of the fourth lead screw 701. The sample needle adapter 703 is fixedly provided with a push block 705, which is coaxially arranged with the sample needle 704. It is an electrically driven structure that can slide along the axial direction of the sample needle adapter 703 to push the sample needle 704 down from the sample needle adapter 703, thereby realizing the automatic detachment of the sample needle 704.

[0031] Working principle The operator injects the pre-treated sample into the biochip to be tested and places the chip into the chip compartment 201; a disposable sample needle 704 is placed in the sample needle storage area of ​​the reagent consumable compartment 205, and the test reagents are placed in the reagent storage area; the safety door of the equipment is closed, and the chip compartment 201 and the reagent consumable compartment 205 are driven by electric guide rails to transport the chip, sample needle, and reagents to the operable station inside the equipment; the equipment starts a full system self-test, the three-axis linkage mechanism of the displacement structure 3 performs origin calibration, the four major execution modules complete lifting and resetting, and the worktable, temperature control module, and liquid level sensor complete functional self-tests. After all self-tests pass, the equipment enters the standby state.

[0032] First, the equipment identifies the chip type and testing requirements through pre-entered chip information and automatically matches the corresponding first workbench 202 or second workbench 204; the displacement structure 3 drives the transverse beam to complete the left and right transverse displacement and drives the mounting frame 302 to complete the front and back longitudinal displacement, thereby moving the gripper module 5 synchronously to directly above the chip in the chip compartment 201.

[0033] Then, the second lead screw 501 drives the gripper module 5 to descend vertically downward along the Z-axis slide rail to the preset gripping position. The drive motor 502 drives the two sets of slide blocks 504 to slide synchronously in opposite directions along the slide groove of the track seat 503, causing the two clamping plates 505 to open synchronously. Subsequently, the equipment control system controls the gripper's drive motor 502 to drive the two clamping plates 505 to close synchronously. The chip position is calibrated by the first protrusion 506, and the rubber strip 507 and the second protrusion 508 adhere to the outer wall of the chip to complete stable gripping.

[0034] Subsequently, the second lead screw 501 drives the gripper module 5 to lift and reset, and the displacement structure 3 drives the transverse beam and mounting frame 302 to move synchronously to the top of the matching target workbench. The gripper module 5 then descends again to place the chip smoothly into the chip positioning slot of the workbench. The clamping plate 505 is released to complete the transfer and positioning of the chip, and the gripper module 5 resets to the standby position.

[0035] The displacement structure 3 drives the transverse beam to complete the left and right lateral displacement and drives the mounting frame 302 to complete the front and back longitudinal displacement, thereby moving the pipetting module 7 synchronously to the top of the sample needle storage area of ​​the reagent consumables compartment 205. The fourth lead screw 701 drives the pipette 702 to descend vertically along the Z-axis slide rail. The downward pressure of the lead screw causes the sample needle adapter 703 to complete the interference fit with the disposable sample needle 704, realizing the automatic assembly of the sample needle 704. Then the pipette 702 is raised and reset.

[0036] Then, the displacement structure 3 drives the transverse beam and the mounting frame 302 to move synchronously to the top of the reagent storage area. The fourth lead screw 701 drives the pipette 702 and the sampling needle 704 to descend until the sampling needle is inserted into the reagent. The pipette 702 draws a preset volume of the test reagent through the sampling needle 704. The fourth lead screw 701 drives the pipette 702 and the sampling needle 704 to rise. The displacement structure 3 drives the sampling needle 704 to move synchronously to the top of the chip on the worktable. The fourth lead screw 701 drives the pipette 702 and the sampling needle 704 to descend to a preset height, controlling the pipette 702 to accurately drip the reagent into the corresponding reaction well of the chip, completing the reagent dispensing. Next, the displacement structure 3 drives the transverse beam and the mounting frame 302 to move the pipette 702 and the sampling needle 704 synchronously to directly above the collection frame, controlling the push block 705 on the sampling needle adapter 703 to move, pushing the sampling needle 704 downward, and the discarded sampling needle falls into the collection frame, slides vertically through the guide cavity into the sampling needle collection box 102 for recycling, and the pipette 702 resets to the standby position.

[0037] Subsequently, the hot cover 203 above the worktable where the chip is located automatically descends and closes, heating the cover on the chip and fully sealing and insulating the reaction holes of the chip. Depending on the experimental conditions of different biochip detection projects, modules such as displacement structure 3, gripper module 5, waste liquid absorption module 6, pipetting module 7, hot cover 203, first worktable 202, and second worktable 204 work together to complete the entire process of hybridization, cleaning, incubation, and color development of the biochip.

[0038] After the reaction process is completed, the hot cover 203 automatically opens, and the displacement structure 3 drives the transverse beam and the mounting frame 302 to move synchronously to the top of the chip. The third lead screw 601 drives the liquid suction seat 602 and the liquid suction needle 604 to descend vertically along the Z-axis slide rail, so that the needle tip of the liquid suction needle 604 makes full contact with the waste liquid in the chip hole. The negative pressure pump on the inner wall of the whole machine starts, and all the waste liquid is drawn into the waste liquid collection box 103 through the liquid suction interface 603 and the delivery pipe. After the waste liquid is drawn, the waste liquid absorption module 6 is reset to the standby position.

[0039] Finally, the displacement structure 3 drives the horizontal beam and the mounting frame 302 to move in tandem, causing the image recognition structure 4 to move synchronously to directly above the chip. The first lead screw 401 drives the industrial camera 402 to descend vertically along the Z-axis slide rail to complete focusing. The light source 403 turns on to provide supplementary light and takes high-definition pictures of the reaction holes of the chip one by one in a preset order, and transmits the pictures to the equipment control system. The equipment control system generates a complete report for this test, which includes core data such as chip information, reaction parameters, test images, and interpretation results.

[0040] After the photo interpretation is completed, the displacement structure 3 drives the horizontal beam and the mounting frame 302 to move synchronously to the top of the workbench, grab the chip that has been tested and transfer it back into the chip compartment 201. Subsequently, the chip compartment 201 and reagent consumable compartment 205 automatically slide out of the compartment via electric guide rails, and the completed biochip, waste liquid storage box and sample needle holder are manually removed; then, all components such as displacement structure 3, gripper module 5, waste liquid absorption module 6, pipetting module 7, and hot cover 203 are reset to the initial standby position, and the equipment enters the standby state for the next test.

[0041] After completing all daily testing experiments, manually activate the instrument maintenance function: turn off the exhaust fan and turn on the ultraviolet sterilization lamp; after the ultraviolet lamp completes the preset sterilization time, turn off the ultraviolet sterilization lamp, turn on the exhaust fan to complete the terminal sterilization and ventilation of the chamber and cabinet 101, and then automatically shut down.

[0042] When the liquid level in the waste liquid collection box 103 reaches the warning value, the equipment sends a signal to the control system through the liquid level sensor, automatically reminding the operator to clean it. The sample needle collection box 102 and the waste liquid collection box 103 can be automatically slid out of the equipment by electric drive, and the operator can complete the centralized harmless treatment of waste consumables and waste liquid.

[0043] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0044] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. An integrated intelligent biochip detection machine, characterized in that, It includes a base structure (1), a worktable structure (2) fixed to the top of the base structure (1), and a displacement structure (3) fixed to the top of the whole machine. The displacement structure (3) includes a gantry frame (301) and a mounting frame (302). The mounting frame (302) is slidably connected to the gantry frame (301) and can move in the full range on the top plane of the workbench structure (2). The mounting frame (302) is equipped with a photo-reading structure (4) for capturing chip detection results, a gripper module (5) for chip transfer, a waste liquid absorption module (6) for waste liquid extraction, and a pipetting module (7) for reagent dispensing. The photo-reading structure (4), gripper module (5), waste liquid absorption module (6), and pipetting module (7) can all move in a plane synchronously with the mounting frame (302), and can all independently complete vertical lifting and lowering movements by being driven by a screw. The image interpretation structure (4) includes a light source (403) and an industrial camera (402). The image interpretation structure (4) can be driven to move vertically by a lead screw. The light source (403) and the industrial camera (402) are arranged coaxially. The illumination direction of the light source (403) is consistent with the focusing direction of the lens of the industrial camera (402). The bottom of the gripper module (5) is provided with two sets of clamps (505) that can be opened and closed synchronously in opposite directions and are arranged oppositely. The base structure (1) is provided with a sealed collection chamber for collecting waste consumables and experimental waste liquid. The workbench structure (2) is fixed with a collection frame that runs vertically through the workbench. The bottom of the collection frame is connected to the top channel of the sealed collection chamber.

2. The intelligent biochip detection integrated machine according to claim 1, characterized in that, The gantry frame (301) is provided with left and right extended X-axis linear slide rails. A transverse beam is slidably connected to the X-axis linear slide rails through a sliding block. A Y-axis linear slide rail is fixed on the transverse beam, which can drive the Y-axis linear slide rail to complete left and right displacement along the X-axis linear slide rail. The mounting frame (302) is connected to the Y-axis linear slide rail through a sliding block and can complete front and rear longitudinal displacement along the Y-axis linear slide rail.

3. The intelligent biochip detection integrated machine according to claim 1, characterized in that, The mounting frame (302) is provided with four independent vertical drive screws: a first screw (401), a second screw (501), a third screw (601), and a fourth screw (701). The photographic interpretation structure (4) is connected to the first screw (401) via a sliding block. The gripper module (5) is connected to the second screw (501) via a sliding block. The waste liquid absorption module (6) is connected to the third screw (601) via a sliding block. The liquid transfer module (7) is connected to the fourth screw (701) via a sliding block.

4. The intelligent biochip detection integrated machine according to claim 3, characterized in that, The image interpretation structure (4) is installed on the outer end of the first side of the mounting frame (302) along the Y-axis direction, the gripper module (5) is installed on the inner end of the same side of the mounting frame (302); the pipetting module (7) is installed on the outer end of the second side of the mounting frame (302) along the length direction, and the waste liquid absorption module (6) is installed on the inner end of the same side of the mounting frame (302).

5. The intelligent biochip detection integrated machine according to claim 3, characterized in that, The gripper module (5) includes a mounting base connected to the second lead screw (501) via a sliding block. A drive motor (502) is fixedly mounted on the bottom of the mounting base, and a track seat (503) is fixedly mounted on the output end of the drive motor (502). A horizontally extending slide groove is provided on the bottom of the track seat (503), and two sets of synchronously opposite sliding seats (504) are slidably connected in the slide groove. A clamping plate (505) is fixedly mounted on the bottom of each set of sliding seats (504).

6. The intelligent biochip detection integrated machine according to claim 5, characterized in that, The inner wall of the clamp (505) is fixed with a first protrusion (506) and a rubber strip (507); a second protrusion (508) is fixed on the rubber strip (507); the first protrusion (506) is a rigid positioning structure and is symmetrically distributed on both sides of the inner wall of the clamp (505); the second protrusion (508) is an arc-shaped rubber protrusion and is evenly arranged along the length direction of the rubber strip (507).

7. The intelligent biochip detection integrated machine according to claim 3, characterized in that, The pipetting module (7) includes a pipette (702) connected to a fourth lead screw (701) via a sliding block. A sample needle adapter (703) is fixedly mounted on the bottom of the pipette (702). A disposable sample needle (704) can be assembled on the sample needle adapter (703) by interference fit. A push block (705) is fixedly mounted on the sample needle adapter (703). The push block (705) is coaxially arranged with the sample needle (704) and is used to push the sample needle (704) down from the sample needle adapter (703).

8. The intelligent biochip detection integrated machine according to claim 3, characterized in that, The waste liquid absorption module (6) includes a suction seat (602) connected to a third lead screw (601) via a sliding block. A suction needle (604) is fixedly provided at the bottom of the suction seat (602), and a suction interface (603) is fixedly provided on the side wall. The suction interface (603) is connected to the internal flow channel of the suction needle (604). A negative pressure pump for providing negative pressure power for waste liquid extraction is fixedly provided on the inner side wall of the whole machine. The inlet end of the negative pressure pump is connected to the suction interface (603) via a delivery pipe, and the outlet end is connected to the sealed collection cavity of the base structure (1) via a delivery pipe.

9. The intelligent biochip detection integrated machine according to claim 1, characterized in that, The workbench structure (2) includes a first workbench (202) and a second workbench (204) fixed side by side. The first workbench (202) is equipped with an adjustable frequency oscillation component and a heating module. The second workbench (204) is equipped with a built-in temperature control module. A liftable heat cover (203) is fixed above the first workbench (202) for heating the cover on the chip. The left side of the workbench structure (2) is equipped with a chip compartment (201), and the right side is equipped with a reagent consumable compartment (205). The chip compartment (201) and the reagent consumable compartment (205) are slidably connected to the workbench structure (2) via electric guide rails.

10. The intelligent biochip detection integrated machine according to claim 1, characterized in that, The base structure (1) includes a cabinet (101), and the sealed collection chamber is located inside the cabinet (101). The sealed collection chamber includes a sample needle collection box (102) and a waste liquid collection box (103) that are independent of each other. Both are installed in the inner cavity of the cabinet (101) by electric sliding rails. A liquid level sensor is fixed on the outer wall of the waste liquid collection box (103). The inner cavity of the cabinet (101) is also provided with a sterilization component and a heat dissipation component.

Images