Cosmetic composition and method of making same

Abstract

This invention provides a cosmetic ingredient and a method for preparing the same. The method includes preparing a pineapple leaf extract. The steps for preparing the pineapple leaf extract include: providing pineapple leaves; pulverizing the pineapple leaves to form pineapple leaf powder; extracting the pineapple leaf powder using an alcohol-water solution and collecting the resulting extract; and sterilizing the extract to obtain the pineapple leaf extract.

Description

Technical Field

[0001] This invention relates to compositions and methods of their preparation, particularly to cosmetic compositions and methods of their preparation. Background Technology

[0002] Pineapple is a popular fruit, but its large-scale cultivation has led to the problem of pineapple leaf waste disposal. Pineapple leaves are not easily composted, resulting in the occupation of agricultural land resources. Burning pineapple leaves may cause air pollution and carbon emissions.

[0003] Therefore, there is still an urgent need to find environmentally friendly solutions for handling pineapple leaf waste. Summary of the Invention

[0004] An embodiment of the present invention provides a method for preparing a cosmetic ingredient. The method includes preparing a pineapple leaf extract. The steps for preparing the pineapple leaf extract include: providing pineapple leaves; pulverizing the pineapple leaves to obtain pineapple leaf powder; extracting the pineapple leaf powder with an alcohol-water solution and collecting the resulting extract; and sterilizing the extract to obtain the pineapple leaf extract.

[0005] Another embodiment of the present invention provides a cosmetic composition prepared by the aforementioned method, wherein the cosmetic composition comprises 1-5 wt% pineapple leaf extract and 95-99 wt% water. The total polyphenol content of the pineapple leaf extract is between 150 mg GAE / 100g pineapple leaf and 550 mg GAE / 100g pineapple leaf. Attached Figure Description

[0006] Figure 1 This illustration shows a method for preparing pineapple leaf extract according to an embodiment of the present invention;

[0007] Figure 2 This plot shows the percentage of antioxidant activity of pineapple leaf extracts in embodiments C1-C3 of the present invention;

[0008] Figure 3 This is a diagram illustrating the results of collagen secretion in Comparative Example D and Examples D1-D3; and

[0009] Figure 4 The results show the collagen secretion of comparative examples E1-E3 and example E1.

[0010] In the attached figures, the following labels are used:

[0011] S1~S7: Steps Detailed Implementation

[0012] The following description, in conjunction with the accompanying drawings, details various embodiments. The description and drawings are provided for illustrative purposes only and are not intended to be limiting. For clarity, some elements and / or symbols may be omitted in some drawings. Furthermore, elements in the drawings may not be drawn to scale. It is anticipated that elements and features in one embodiment can be advantageously incorporated into another embodiment without further repetition.

[0013] This invention relates to a cosmetic composition and its preparation method. The cosmetic composition comprises 1-5 wt% pineapple leaf and 95-99 wt% water, wherein the total polyphenol content of the pineapple leaf extract is between 150 mg GAE / 100g pineapple leaf and 550 mg GAE / 100g pineapple leaf. "GAE" represents gallic acid equivalent, measured based on the weight of 100g pineapple leaf.

[0014] Because the pineapple leaf extract of the present invention has a high total polyphenol content, the cosmetic composition can have excellent antioxidant activity and achieve excellent beauty effects, such as anti-aging and collagen proliferation.

[0015] According to some embodiments, the pineapple leaf extract includes one or more active ingredients, which include total saccharides, ferulic acid, chlorogenic acid (CGA), p-coumaric acid (PCA), or any combination thereof. For example, based on the weight of 100 g of pineapple leaves, the weight of total saccharides is 1-5 g, the weight of ferulic acid is 0.1-30 mg, the weight of PCA is 0.1-30 mg, and the weight of chlorogenic acid is 0.1-30 mg. It should be understood that the active ingredients of pineapple leaves in this invention are not limited thereto. The weight of total saccharides was determined by the phenol-sulfuric acid (PSA) method. The weights of ferulic acid, chlorogenic acid, and PCA were obtained by HPLC.

[0016] According to some embodiments, pineapple leaf extract contains cosmetic ingredients that enhance antioxidant activity and / or promote collagen secretion.

[0017] According to some embodiments, the present invention also provides a method for preparing a cosmetic ingredient. The method includes preparing a pineapple leaf extract. The steps of preparing the pineapple leaf extract include: providing pineapple leaves; pulverizing the pineapple leaves to obtain pineapple leaf powder; extracting the pineapple leaf powder using an aqueous alcohol solution and collecting the resulting extract; and sterilizing the extract to obtain the pineapple leaf extract.

[0018] like Figure 1 As shown, the method for preparing pineapple leaf extract according to an embodiment of the present invention includes the following steps S1 to S7 in sequence.

[0019] In step S1, pineapple leaves are provided as raw material. The appearance, heavy metal residues, pesticide residues, or other testing items of the pineapple leaves can be inspected, and must meet relevant regulatory requirements.

[0020] In step S3, a pulverization step is performed, for example using a high-speed centrifugal pulverizer at a speed of 6000 rpm to 18000 rpm, a ring sieve size of 0.08 mm to 1 mm, and a temperature of 25±2 ℃, to pulverize the pineapple leaves to an average particle size of 0.01 to 0.8 mm (e.g., 0.04 to 0.25 mm) to form pineapple leaf powder.

[0021] Optionally, before the crushing step S3, the pineapple leaves can be dried (low-temperature drying process), for example, by using a cyclone dryer at 50~70°C (e.g., 60°C) with a feed rate of 0.2 kgs. -1 The pineapple leaves are dried under specific conditions. The moisture content of the pineapple leaves after the drying process is no more than 10% (by weight on a dry basis).

[0022] In step S5, an extraction step is performed. For example, the aforementioned pineapple leaf powder can be extracted with an aqueous alcohol solution (e.g., a 30% aqueous ethanol solution) for 1 hour, and then repeated once, and the resulting extract is collected. In this embodiment, the aqueous alcohol solution is composed of water and ethanol, wherein ethanol accounts for approximately 30% (v / v%) of the total volume of the aqueous alcohol solution, for example, 25-35 v / v%. However, the types of alcohols and their volume percentage ranges in this invention are not limited to this. It should be understood that other suitable solvents can also be used for extraction.

[0023] In step S7, the aforementioned extract is sterilized. For example, a filter syringe with pore sizes of 0.45 μm and 0.22 μm can be used to perform two sterilization filtrations to obtain pineapple leaf extract.

[0024] It should be understood that the method for preparing the pineapple leaf extract of the present invention may also include other steps, but is not limited thereto.

[0025] Compared to the comparative example without the pulverization step, the pineapple leaf extract of one embodiment of the present invention, due to the pulverization process, yields pineapple leaf powder with a smaller particle size (e.g., between 0.01 mm and 0.5 mm), thereby possessing a higher specific surface area and improving the extraction efficiency of organic solvents. Therefore, more active ingredients can be extracted in subsequent extraction steps, such as higher total polyphenol, chlorogenic acid, and p-coumaric acid content, exhibiting better antioxidant activity and superior collagen-boosting effects.

[0026] Compared to a comparative example using a conventional oven drying method, the pineapple leaf extract of this embodiment is dried using a cyclone-type low-temperature drying process, which retains more active ingredients (such as coumaric acid and chlorogenic acid) and reduces the damage to these active ingredients caused by high temperatures. Therefore, the pineapple leaf extract obtained by this invention has superior cosmetic effects. For example, its total polyphenol content can be increased by approximately 50 times, and its antioxidant capacity can be further enhanced (approximately 55% increase in the antioxidant test using ABTS reagent and approximately 20.15% increase in the antioxidant test using DPPH reagent; ABTS stands for 2,2'-azinobis-3-ethyl benzothiazoline-6-sulphonic acid; DPPH stands for 2,2-diphenyl-1-picrylhydrazyl).

[0027] To make the above and other objects, features, and advantages of the present invention more apparent and understandable, several embodiments are described in detail below:

[0028] Antioxidant test

[0029] According to some embodiments, the pineapple leaf extract can be subjected to an ABTS radical scavenging assay. Specifically, the ABTS radical scavenging assay can be used to assess the antioxidant capacity of the sample. ABTS reacts with potassium persulfate (K₂S₂O₈) to produce an oxidation reaction, forming a stable blue-green water-soluble ABTS· + Free radical ions have a maximum absorption peak at 734 nm, and therefore can pass through A 734 nm The concentration of ABTS free radicals was detected. When an antioxidant (free radical scavenger) was added to the ABTS free radical solution, the originally singular free radicals were paired, causing the ABTS free radical solution to decolorize and absorb A. 734 nm The absorption value decreases, thereby assessing the free radical scavenging ability of antioxidants.

[0030] This experiment was conducted according to the method described by Arnao et al. (ARNAO MB, CANO A, HERNANDEZ-RUIZ J, GARCIA-CANOVAS F, ACOSTA M (1996) Inhibition by L-ascorbic acid and other antioxidants of the 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) oxidation catalyzed by peroxidase: a new approach for determining total antioxidant status of foods. Anal Biochem 236: 255-261) after modification. ABTS (brand: Sigma) was dissolved in deionized water (DI water) at room temperature and the concentration was adjusted to 7 mM. Then, 2.45 mM potassium persulfate was added, and the mixture was placed in a dark room at room temperature for 16 hours to generate stable ABTS radical ions (blue-green). The solution was then diluted with deionized water to obtain a working solution of ABTS radical ions with an absorbance of 0.75 ± 0.05 at 734 nm using a spectrophotometer.

[0031] Next, the test sample and standards of different concentrations (Trolox) were added to the ABTS free radical ion working solution, with deionized water added as the control group. After reacting for 20 minutes, the absorbance at 734 nm was measured using a spectrophotometer. The scavenging ability of different concentrations of standards (Trolox) against ABTS free radical ions was used to plot a scavenging rate standard curve. The absorbance values ​​measured for the test sample and the control group were converted according to this standard curve to obtain the free radical scavenging ability. Free radical scavenging ability represents antioxidant activity, and its calculation formula is shown in Equation 1 below:

[0032] (1-ABS sample / ABS control Formula 1 (1 / 100)

[0033] Among them, ABS sample Indicates the absorbance value of the sample being tested, ABS control This indicates the absorbance value of the control group.

[0034] Please refer to Figure 2The graph illustrates the percentage of antioxidant activity of the pineapple leaf extracts in Examples C1-C3, measured by the ABTS free radical scavenging test as described above. The concentrations of the pineapple leaf extracts in Examples C1-C3 were 2 wt%, 1 wt%, and 0.50 wt%, respectively. The experimental results show that the antioxidant activities of Examples C1-C3 were 99.82%, 99.95%, and 96.64%, respectively. Examples C1-C2 had higher pineapple leaf extract concentrations and better antioxidant activity compared to Example C3.

[0035] According to some embodiments, the antioxidant capacity of extracts can be assessed by measuring the total polyphenol content (TPC). Generally, the higher the total polyphenol content of pineapple leaf extract, the stronger its antioxidant capacity. Please refer to Table 1 below, which shows the total polyphenol content and antioxidant activity results of Comparative Example A and Examples A1 and A2. The total polyphenol content is calculated based on gallic acid equivalent (GAE) and is shown as the total polyphenol content of pineapple leaf extract per 100 g of pineapple leaves (mg GAE / 100 g pineapple leaves). Antioxidant activity was determined by the ABST free radical scavenging test as described above, and the antioxidant activity in Table 1 represents the ABST activity at a concentration of 0.5 wt% of pineapple leaf extract.

[0036] Examples A1 and A2 are prepared according to the aforementioned steps S1 to S7 (e.g. Figure 1 (As shown). The difference between Comparative Example A and Examples A1 and A2 is that Comparative Example A did not perform the pulverization step (i.e., step S3), while all other processing steps were the same. The average particle size of the pineapple leaf powder obtained after the pulverization step in Example A1 was 0.25 mm, and the average particle size of the pineapple leaf powder obtained after the pulverization step in Example A2 was 0.04 mm.

[0037] Table 1

[0038] Group Total polyphenols (mg GAE / 100 g pineapple leaves) Antioxidant activity (%) - Extract concentration 0.5wt% Comparative Example A 133.9 55.9 Example A1 180.2 67.2 Example A2 428.9 96.64

[0039] According to some embodiments, the antioxidant capacity of extracts can be assessed by measuring the content of specific polyphenols. For example, higher contents of ferulic acid, chlorogenic acid (CGA), and p-coumaric acid (PCA) in pineapple leaf extracts indicate stronger antioxidant capacity. Please refer to Tables 2 and 3 below, where Table 2 shows the contents of ferulic acid and PCA in Comparative Example B and Examples B1-B2, and Table 3 shows the contents of PCA and chlorogenic acid in Comparative Example B and Example B3. Examples B1-B3 were prepared according to the aforementioned steps S1-S7 (e.g., ...). Figure 1 As shown in the figure, Example B1 was sieved through a 0.5 mm sieve, while Examples B2-B3 were sieved through a 0.08 mm sieve. The difference between Comparative Example B and Examples B1-B3 is that Comparative Example B did not perform the pulverization step (i.e., step S3), while all other processing steps were the same. The average particle size of the pineapple leaf powder obtained in Comparative Example B is larger than that obtained in Examples B1-B3, and the average particle size of the pineapple leaf powder obtained in Example B1 is larger than that obtained in Examples B2-B3.

[0040] Table 2

[0041] Group Ferulic acid (mg / 100g pineapple leaves) Paracoumaric acid (mg / 100g pineapple leaves) Total polysaccharides (g / 100g pineapple leaves) Comparative Example B 19.79 14.66 1.56 Example B1 20.24 19.74 1.72 Example B2 22.26 21.58 2.58

[0042] Table 3

[0043] Group Chlorogenic acid (mg / 100g pineapple leaves) Comparative Example B 13.88 Example B3 14.62

[0044] As shown in Tables 1, 2, and 3, after the pulverization step, the pineapple leaf extract has higher contents of total polyphenols, total polysaccharides, ferulic acid, chlorogenic acid, and p-coumaric acid, and its antioxidant activity is also improved. Specifically, ferulic acid increased by 12.5%, p-coumaric acid by 47.2%, total polysaccharides by 65%, and chlorogenic acid by 5.3%, indicating that the extract of the present invention possesses superior antioxidant capacity. Furthermore, as shown in Table 2, Example B2 has more active ingredients (i.e., total polysaccharides, ferulic acid, and p-coumaric acid) than Example B1, indicating that a smaller average particle size of the pineapple leaf powder promotes the extraction of active ingredients, thereby increasing the content of active ingredients in the pineapple leaf extract.

[0045] Collagen proliferation test

[0046] According to some embodiments, collagen proliferation can be induced by pineapple leaf extract (cell experiments) to conduct collagen proliferation assays. The cells used in the experiments were HSF cell lines of human skin fibroblasts purchased from the Animal Science Institute for this in vitro assay analysis.

[0047] The above-mentioned human skin fibroblast cell line HSF was seeded at 3 x 10⁵ cells / well in a 6-well cell culture dish. After the cells attached, different concentrations of pineapple leaf extract or other samples were added to the cells for co-culture, with the control group not added.

[0048] After 7 days of co-culture, the supernatant from each well was collected and the collagen secretion was analyzed using a collagen analysis kit (S1000, Biocolor) according to the instructions. The results are as follows: Figures 3-4 As shown.

[0049] Please refer to Figure 3 The results show the collagen secretion of Comparative Example D and Examples D1-D3. The Y-axis represents the relative percentage of collagen (i.e., the collagen concentration of Comparative Example D is used as 100% as a baseline, and the collagen concentration of Examples D1-D3 is calculated accordingly compared to Comparative Example D). Comparative Example D (as a control group) did not contain pineapple leaf extract, while Examples D1-D3 contained 0.2 wt%, 0.5 wt%, and 1.0 wt% of pineapple leaf extract, respectively.

[0050] like Figure 3 As shown, compared to Comparative Example D, Examples D1 to D3 all increased collagen secretion.

[0051] Please refer to Figure 4 The results show the collagen secretion of Comparative Examples E1-E3 and Example E1 as determined by the collagen hyperplasia assay shown above. The Y-axis represents the relative percentage of collagen (i.e., using Comparative Example E1 as the control group and its collagen concentration as 100%, the collagen concentration of Comparative Examples E2-E3 and Example E1 compared to Comparative Example E1 is calculated). Example E1 uses 0.2 wt% pineapple leaf extract of the present invention to induce collagen hyperplasia. The difference between Comparative Examples E1-E3 and Example E1 is that Comparative Example E1 is a control group without the addition of pineapple leaf extract and other active ingredients, Comparative Example E2 has 0.3 wt% histidine added, and Comparative Example E3 has 0.2 wt% modified hyaluronic acid (commercially available, HYA-HEAL) added. + ).

[0052] like Figure 4 As shown, Example E1 has a higher collagen concentration than Comparative Examples E1-E2, and its collagen concentration is similar to that of Comparative Example E3.

[0053] comprehensive Figures 3-4The results show that the pineapple leaf extract of the present invention has the ability to induce collagen proliferation, and its effect is comparable to that of commercially available products (such as modified hyaluronic acid), indicating that the present invention has potential cosmetic application value and helps prevent skin aging.

[0054] Please refer to Table 4 below, which shows the amount of collagen secreted in Comparative Example F and Example F1 as determined by the collagen proliferation assay shown above. The pineapple leaf extract of Example F1 was prepared by steps S1-S7 as described above (e.g., Figure 1 (As shown). The difference between Comparative Example F and Example F1 is that Comparative Example F did not perform the pulverization step (i.e., step S3), while all other processing steps were the same. The average particle size of the pineapple leaf powder obtained after the pulverization step in Example F1 was 0.25 mm.

[0055] Table 4

[0056] Group Collagen secretion (relative percentage) Comparative Example F 74.69 Example F1 130.64

[0057] As shown in Table 4, after the pulverization step, the pineapple leaf extract has a higher collagen concentration, which can enhance the collagen proliferation capacity.

[0058] In summary, this invention provides a cosmetic composition and a method for preparing the same. The cosmetic composition includes a pineapple leaf extract. Experimental results have confirmed that the pineapple leaf extract possesses various beauty-related effects, such as enhancing antioxidant capacity and promoting collagen secretion.

[0059] While the present invention has been disclosed above by way of embodiments, it is not intended to limit the invention. Those skilled in the art can make various modifications and refinements without departing from the spirit and scope of the invention. Therefore, the scope of protection of the present invention shall be determined by the scope defined in the appended claims.

Claims

1. A method for preparing a cosmetic ingredient, comprising: The steps for preparing pineapple leaf extract include: Provide pineapple leaves; The pineapple leaf is pulverized into pineapple leaf powder. The pineapple leaf powder was extracted using an aqueous alcohol solution, and the resulting extract was collected. The extract was sterilized to obtain the pineapple leaf extract.

2. The method for preparing the cosmetic composition according to claim 1, wherein, The particle size of the pineapple leaf powder is between 0.01 mm and 0.8 mm.

3. The method for preparing the cosmetic composition according to claim 1, wherein, The pulverizing step is carried out using a high-speed centrifugal pulverizer.

4. The method for preparing the cosmetic composition according to claim 1, wherein, The process of preparing this pineapple leaf extract also includes a drying step to dry the pineapple leaves.

5. The method for preparing the cosmetic composition according to claim 4, wherein, The temperature for this drying step is 50℃~70℃.

6. The method for preparing a cosmetic composition according to claim 4, wherein, The moisture content of the pineapple leaves after this drying step is no more than 10%.

7. The method for preparing a cosmetic composition according to claim 1, wherein, The aqueous solution of this alcohol is a 30% ethanol aqueous solution.

8. A cosmetic composition prepared by the method according to any one of claims 1 to 7, wherein the cosmetic composition comprises: 1~5 wt% pineapple leaf extract; as well as The extract contains 95-99 wt% water, and the total polyphenol content of the pineapple leaf extract is between 150 mg GAE / 100g pineapple leaf and 550 mg GAE / 100g pineapple leaf.

9. The cosmetic composition according to claim 8, wherein, The pineapple leaf extract contains one or more active ingredients, including total saccharides, ferulic acid, chlorogenic acid (CGA), p-coumaric acid (PCA), or any combination thereof.

10. The cosmetic composition according to claim 9, wherein, Based on the weight of 100 g of pineapple leaves, the total polysaccharide that can be extracted is between 1 and 5 g, the ferulic acid is between 0.1 and 30 mg, the coumaric acid is between 0.1 and 30 mg, and the chlorogenic acid is between 0.1 and 30 mg.

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