Preparation method and application of a composition for promoting growth and resisting inflammation of egg chicks

Abstract

This invention relates to a method for preparing and applying a composition for promoting growth and reducing inflammation in laying chicks, belonging to the field of veterinary feed additives. The components and their weight ratios are as follows: 20-30 parts of Codonopsis pilosula extract, 15-25 parts of Astragalus membranaceus extract, 10-20 parts of Tartary buckwheat extract, 10-20 parts of Elsholtzia ciliata extract, 3-5 parts of zinc sucrose, 2-3 parts of emulsifier, and 1-2 parts of stabilizer. First, the four extracts of Codonopsis pilosula, Astragalus membranaceus, Tartary buckwheat, and Elsholtzia ciliata are concentrated, spray-dried, and then mixed evenly with zinc sucrose, emulsifier, and stabilizer using a three-dimensional mixer to obtain the composition for promoting growth and reducing inflammation in laying chicks. This composition can significantly improve the growth performance of laying chicks, enhance their immunity, effectively prevent and control inflammatory responses, and improve survival rate and feed conversion rate. It is suitable for laying chick farming and has broad market application prospects.

Description

Technical Field

[0001] This invention belongs to the field of veterinary feed additives. In particular, it relates to a method for preparing and applying a composition for promoting growth and reducing inflammation in laying chicks. Background Technology

[0002] In poultry farming, the growth rate and health status of chicks directly affect farming efficiency and product quality. Traditional feeding methods often rely on large amounts of antibiotics and chemical additives to promote growth and prevent disease. However, these methods may not only lead to food safety issues but also negatively impact the immune system and gut health of chicks.

[0003] In recent years, with increasing awareness of food safety and healthy farming, more and more research has focused on the application of natural plant extracts in poultry farming. Traditional Chinese medicines such as Codonopsis pilosula, Astragalus membranaceus, Tartary buckwheat, and Elsholtzia ciliata possess various bioactive components, including polysaccharides, flavonoids, and volatile oils. These components exhibit significant effects in promoting animal growth, enhancing immunity, and providing anti-inflammatory and antioxidant benefits. Furthermore, sucrose zinc, as an organic zinc source, not only provides the zinc needed by animals but also has better bioavailability and fewer side effects. However, simply mixing the aforementioned natural plant extracts with sucrose zinc and other components cannot fully realize their synergistic effects and may result in poor stability and low absorption rates in practical applications. Therefore, a scientifically sound preparation method is needed to effectively combine these components to form a stable, efficient, and easily absorbed growth-promoting and anti-inflammatory composition. Summary of the Invention

[0004] The purpose of this invention is to provide a composition for promoting growth and reducing inflammation in laying chicks. The formulation of this invention is scientifically and rationally composed, which can significantly improve the growth performance of laying chicks, enhance their immunity, effectively prevent and control inflammatory responses, and improve survival rate and feed conversion rate. It is suitable for raising laying chicks at different scales.

[0005] Another object of the present invention is to provide a method for preparing a tetravalent attenuated live vaccine composition for chicken coccidiosis.

[0006] To achieve the above objectives, the present invention provides the following technical solution: This invention relates to a method for preparing and applying a composition for promoting growth and reducing inflammation in laying hens, characterized in that the components and their weight ratios are as follows: 20-30 parts of Codonopsis pilosula extract, 15-25 parts of Astragalus membranaceus extract, 10-20 parts of Tartary buckwheat extract, 10-20 parts of Elsholtzia ciliata extract, 3-5 parts of zinc sucrose, 2-3 parts of emulsifier, and 1-2 parts of stabilizer.

[0007] Preferably, the composition comprises the following components, wherein the optimal proportions are: Codonopsis pilosula extract 25 parts, Astragalus membranaceus extract 20 parts, Tartary buckwheat extract 15 parts, Elsholtzia ciliata extract 15 parts, zinc sucrose 5 parts, emulsifier 3 parts, stabilizer 2 parts.

[0008] Furthermore, the emulsifier is one of lecithin, glyceryl monostearate, or sucrose fatty acid ester, and the stabilizer is one of sodium alginate or carrageenan.

[0009] A method for preparing a composition for promoting growth and reducing inflammation in laying chicks, comprising the following steps: (1) Take the extracts of Codonopsis pilosula and Astragalus membranaceus according to the weight ratio, add water and stir until dissolved to obtain mixed solution A, then place it in a vacuum concentrator connected to a vacuum pump and a condenser, control the temperature at 60-70℃, set the pressure at -0.08 to -0.1MPa, and concentrate the volume to 1 / 5 to 1 / 4 of solution A.

[0010] (2) Take the buckwheat extract according to the weight ratio, add water and stir until dissolved to obtain mixed solution B, then place it in a concentrator connected to a vacuum pump and a condenser, control the temperature at 35 to 50°C, set the pressure at -0.08 to -0.1 MPa, and concentrate the volume to 1 / 5 to 1 / 4 of solution B.

[0011] (3) Take the extract of Elsholtzia ciliata by weight ratio, add water and stir until dissolved to obtain mixed solution C, then place it in a concentrator connected with a vacuum pump and a condenser, control the temperature at 30 to 45°C, set the pressure at -0.08 to -0.1 MPa, and concentrate the volume to 1 / 4 of solution C.

[0012] (4) The four concentrated liquids obtained in steps (1), (2) and (3) are spray-dried respectively. The inlet temperature of the spray dryer is set to 150-180℃ and the outlet temperature is controlled at 70-80℃ to ensure that the moisture content of the powder is below 5%. Then the dried powder is sieved through a 200-mesh sieve to remove large particles and impurities, and uniformly sized Codonopsis pilosula and Astragalus membranaceus powder a, buckwheat powder b, and Elsholtzia c are obtained in sequence.

[0013] (5) Take zinc sucrose, emulsifier and stabilizer in the weight ratio, and put them together with powders a, b and c obtained in step (4) of claim 3 into a three-dimensional mixer for mixing. The speed is 20-30 r / min and the mixture is mixed for 30 min to obtain mixture D.

[0014] (6) Dispense the mixture D obtained in step (5) into aluminum foil bags and seal them. A composition for promoting growth and reducing inflammation in egg-laying chicks can be obtained.

[0015] Furthermore, the emulsifier is one of lecithin, glyceryl monostearate, or sucrose fatty acid ester.

[0016] Furthermore, the stabilizer is either sodium alginate or carrageenan.

[0017] 1. The composition provided by this invention for promoting growth and reducing inflammation in laying hens contains natural plants that possess both nutritional and medicinal benefits. The polysaccharides, saponins, and schisandric acid in Codonopsis pilosula can enhance poultry immunity and disease resistance, as well as stimulate intestinal peristalsis, improve digestibility and absorption, and promote growth and development. Astragalus membranaceus has qi-tonifying and yang-raising properties; its polysaccharides are rich in various amino acids, vitamins, and trace elements, and can be used as a nutritional additive to improve appetite and promote growth. The bioflavonoids in buckwheat have anti-inflammatory and antioxidant properties, helping poultry resist free radical damage. Elsholtzia ciliata extract promotes digestive enzyme secretion and has antimicrobial, antioxidant, anti-inflammatory, antispasmodic, and analgesic effects. Through a scientifically sound preparation method, the components are effectively combined to promote poultry growth and development, improve feed conversion rate, and enhance the poultry's anti-inflammatory and antioxidant capabilities.

[0018] 2. Sucrose zinc, as an organic zinc source, is an indispensable trace element in the growth and development of chicks. It participates in the synthesis and activation of various enzymes in chicks, can be effectively absorbed and utilized, promotes the growth and development of chicks, enhances immunity, improves feed utilization, and increases their disease resistance and survival rate.

[0019] 3. The composition for promoting growth and reducing inflammation in egg-laying chicks provided by this invention uses natural plant extracts and components such as zinc sucrose. It contains no antibiotics or chemical additives, is safe and environmentally friendly, and meets the requirements of modern healthy farming. Detailed Implementation

[0020] The present invention is further described in detail through the following embodiments. It should be noted that the following embodiments are illustrative and not limiting, and should not be used to limit the scope of protection of the present invention.

[0021] The herbal extracts, zinc sucrose, emulsifiers, and stabilizers used in this invention are all commercially available. Example

[0022] This embodiment provides a composition for promoting growth and reducing inflammation in laying hens, made from the following raw materials in parts by weight: 25 parts Codonopsis pilosula extract, 20 parts Astragalus membranaceus extract, 15 parts Tartary buckwheat extract, 15 parts Elsholtzia ciliata extract, 5 parts zinc sucrose, 3 parts emulsifier, and 2 parts stabilizer. The emulsifier is one of lecithin, glyceryl monostearate, or sucrose fatty acid ester, and the stabilizer is one of sodium alginate or carrageenan. In this embodiment, the emulsifier is lecithin, and the stabilizer is sodium alginate.

[0023] A method for preparing a composition for promoting growth and reducing inflammation in laying chicks, comprising the following steps: (1) Take the extracts of Codonopsis pilosula and Astragalus membranaceus according to the weight ratio, add water and stir until dissolved to obtain mixed solution A, then place it in a vacuum concentrator connected to a vacuum pump and a condenser, control the temperature at 70℃, set the pressure at -0.08 to -0.1MPa, and concentrate the volume to 1 / 5 of solution A.

[0024] (2) Take the buckwheat extract according to the weight ratio, add water and stir until dissolved to obtain mixed solution B, then place it in a concentrator connected to a vacuum pump and a condenser, control the temperature at 35°C, set the pressure at -0.08 to -0.1 MPa, and concentrate the volume to 1 / 5 of solution B.

[0025] (3) Take the extract of Elsholtzia ciliata by weight ratio, add water and stir until dissolved to obtain mixed solution C, then place it in a concentrator connected with a vacuum pump and a condenser, control the temperature at 35℃, set the pressure at -0.08 to -0.1MPa, and concentrate the volume to 1 / 4 of solution C.

[0026] (4) The four concentrated liquids obtained in steps (1), (2) and (3) are spray-dried respectively. The inlet temperature of the spray dryer is set to 180℃ and the outlet temperature is controlled at 80℃ to ensure that the moisture content of the powder is below 5%. Then the dried powder is sieved through a 200-mesh sieve to remove large particles and impurities, and uniformly sized Codonopsis pilosula and Astragalus membranaceus powder a, buckwheat powder b, and Elsholtzia c are obtained in sequence.

[0027] (5) Take zinc sucrose, lecithin emulsifier and sodium alginate stabilizer according to the weight proportions, and put them together with powders a, b and c obtained in step (4) of claim 3 into a three-dimensional mixer for mixing. The speed is 30 r / min and the mixture is mixed for 30 min to obtain mixture D.

[0028] (6) Dispense the mixture D obtained in step (5) into aluminum foil bags and seal them. A composition for promoting growth and reducing inflammation in egg-laying chicks can be obtained. Example

[0029] This embodiment provides a composition for promoting growth and reducing inflammation in laying hens, made from the following raw materials in parts by weight: 20 parts Codonopsis pilosula extract, 15 parts Astragalus membranaceus extract, 10 parts Tartary buckwheat extract, 10 parts Elsholtzia ciliata extract, 3 parts zinc sucrose, 3 parts emulsifier, and 2 parts stabilizer. The emulsifier is one of lecithin, glyceryl monostearate, or sucrose fatty acid ester, and the stabilizer is one of sodium alginate or carrageenan. In this embodiment, the emulsifier is glyceryl monostearate, and the stabilizer is carrageenan.

[0030] A method for preparing a composition for promoting growth and reducing inflammation in laying chicks, comprising the following steps: (1) Take the extracts of Codonopsis pilosula and Astragalus membranaceus according to the weight ratio, add water and stir until dissolved to obtain mixed solution A, then place it in a vacuum concentrator connected to a vacuum pump and a condenser, control the temperature at 60℃, set the pressure at -0.08 to -0.1MPa, and concentrate the volume to 1 / 5 of solution A.

[0031] (2) Take the buckwheat extract according to the weight ratio, add water and stir until dissolved to obtain mixed solution B, then place it in a concentrator connected to a vacuum pump and a condenser, control the temperature at 35°C, set the pressure at -0.08 to -0.1 MPa, and concentrate the volume to 1 / 5 of solution B.

[0032] (3) Take the extract of Elsholtzia ciliata by weight ratio, add water and stir until dissolved to obtain mixed solution C, then place it in a concentrator connected with a vacuum pump and a condenser, control the temperature at 30°C, set the pressure at -0.08 to -0.1 MPa, and concentrate the volume to 1 / 4 of solution C.

[0033] (4) The four concentrated liquids obtained in steps (1), (2) and (3) are spray-dried respectively. The inlet temperature of the spray dryer is set to 150℃ and the outlet temperature is controlled at 70℃ to ensure that the moisture content of the powder is below 5%. Then the dried powder is sieved through a 200-mesh sieve to remove large particles and impurities, and uniformly sized Codonopsis pilosula and Astragalus membranaceus powder a, buckwheat powder b, and Elsholtzia c are obtained in sequence.

[0034] (5) Take zinc sucrose, glyceryl monostearate emulsifier and carrageenan stabilizer according to the weight proportions, and put them together with powders a, b and c obtained in step (4) of claim 3 into a three-dimensional mixer for mixing. The speed is 20 r / min and the mixture is mixed for 30 min to obtain mixture D.

[0035] (6) Dispense the mixture D obtained in step (5) into aluminum foil bags and seal them. A composition for promoting growth and reducing inflammation in egg-laying chicks can be obtained. Example

[0036] This embodiment provides a composition for promoting growth and reducing inflammation in laying hens, made from the following raw materials in parts by weight: 30 parts of Codonopsis pilosula extract, 25 parts of Astragalus membranaceus extract, 15 parts of Tartary buckwheat extract, 18 parts of Elsholtzia ciliata extract, 4 parts of zinc sucrose, 3 parts of emulsifier, and 2 parts of stabilizer. The emulsifier is one of lecithin, glyceryl monostearate, or sucrose fatty acid ester, and the stabilizer is one of sodium alginate or carrageenan. In this embodiment, the emulsifier is sucrose fatty acid ester, and the stabilizer is carrageenan.

[0037] The preparation method is the same as in Example 1.

[0038] Experiment 1 Experimental objective: To investigate the effects of the composition of the present invention on the growth and disease resistance of laying hens, and to determine the optimal dosage.

[0039] Experimental location: Wuqing cooperative breeding farm of Tianjin Herleinte Biotechnology Co., Ltd. Experimental Methods: 240 healthy 5-day-old chicks of similar weight were randomly divided into four groups from the same chicken coop: a control group and low-, medium-, and high-dose experimental groups, with 60 chicks in each group. The control group was fed a basal diet, while the experimental groups were fed a basal diet supplemented with low, medium, and high doses of the composition of this invention, respectively. All four groups of chickens were raised in the same environment with identical temperature, humidity, and light conditions, and had free access to feed and water. The experiment lasted for 8 weeks.

[0040] Data recording: Weekly weight of chicks was recorded; daily health status was observed and disease occurrences were recorded; survival rate was calculated at the end of the experiment. Detailed comparisons of each group are shown in Table 1.

[0041] Table 1. Comparison of Experiments Experimental conclusion: The composition of this invention can promote the growth of laying chicks, reduce the incidence of diseases, and improve the survival rate. Considering all indicators, the medium-dose group represents the optimal addition amount of this composition to laying chick feed.

[0042] Experiment 2 Experimental objective: To investigate the effects of the composition of the present invention on the growth and immunity of chicks, and to determine the optimal dosage.

[0043] Experimental location: Baodi Cooperative Breeding Farm of Tianjin Herleinte Biotechnology Co., Ltd. Experimental Methods: Three hundred healthy, age-matched chicks were randomly divided into six groups of 50 each: a control group, a low-dose group, a medium-dose group, a high-dose group, and two market product comparison groups (added according to the product's recommended dosage). All chicks were kept in the same environment with consistent temperature, humidity, and light conditions, and had free access to feed and water. The experiment lasted for eight weeks.

[0044] Data recording: Daily observation of health status, detailed measurement of various indicators, and recording of disease occurrence; statistical analysis of all data at the end of the experiment. Detailed comparisons of each group are shown in Tables 2-4. Table 2. Comparison of Experiments Table 3 Immune Organ Index Table 4 Immunoglobulin levels (mg / dL) Experimental conclusion: The composition of the present invention can promote the growth of chicks and enhance their immunity, with the medium dose group showing the best performance in promoting the growth of chicks and enhancing their immunity.

[0045] Based on the comprehensive experimental results, the medium-dose group represents the optimal addition amount of this composition in the feed of laying hens.

Claims

1. This invention relates to a method for preparing and applying a composition for promoting growth and reducing inflammation in laying hens, characterized in that: The components and their proportions in the composition are as follows: 20-30 parts of Codonopsis pilosula extract, 15-25 parts of Astragalus membranaceus extract, 10-20 parts of Tartary buckwheat extract, 10-20 parts of Elsholtzia ciliata extract, 3-5 parts of zinc sucrose, 2-3 parts of emulsifier, and 1-2 parts of stabilizer.

2. The composition for promoting growth and reducing inflammation in laying hens according to claim 1, characterized in that: The components of the composition and their optimal proportions are as follows: 25 parts Codonopsis pilosula extract, 20 parts Astragalus membranaceus extract, 15 parts Tartary buckwheat extract, 15 parts Elsholtzia ciliata extract, 5 parts zinc sucrose, 3 parts emulsifier, and 2 parts stabilizer.

3. A method for preparing a composition for promoting growth and reducing inflammation in laying hens as described in claim 1, characterized in that: The preparation method involves the following steps: (1) Take Codonopsis pilosula and Astragalus membranaceus extracts by weight ratio, add water and stir until dissolved to obtain mixed solution A, then place it in a vacuum concentrator connected to a vacuum pump and a condenser, control the temperature at 60-70℃, set the pressure at -0.08 to -0.1MPa, and concentrate the volume to 1 / 5 to 1 / 4 of solution A. (2) Take Tartary buckwheat extract by weight ratio, add water and stir until dissolved to obtain mixed solution B, then place it in a concentrator connected to a vacuum pump and a condenser, control the temperature at 35-50℃, set the pressure at -0.08 to -0.1MPa, and concentrate the volume to 1 / 5 to 1 / 4 of solution B. (3) Take Elsholtzia ciliata extract by weight ratio, add water and stir until dissolved to obtain mixed solution C, then place it in a concentrator connected to a vacuum pump and a condenser, control the temperature at 30-45℃, set the pressure at -0.08 to -0.1MPa, and concentrate the volume to 1 / 4 of solution C. (4) The four concentrated liquids obtained in steps (1), (2), and (3) are spray-dried respectively. The inlet temperature of the spray dryer is set to 150-180℃ and the outlet temperature is controlled at 70-80℃ to ensure that the moisture content of the powder is below 5%. Then, the dried powder is sieved through a 200-mesh sieve to remove large particles and impurities, and uniformly sized Codonopsis pilosula and Astragalus membranaceus powder a, buckwheat powder b, and Elsholtzia c are obtained in sequence. (5) Sucrose zinc, emulsifier, and stabilizer are taken in the weight ratio and put into a three-dimensional mixer together with powders a, b, and c obtained in step (4) of claim 3 for mixing. The speed is 20-30 r / min and the mixture is mixed for 30 min to obtain mixture D. (6) Mixture D obtained in step (5) is packaged into aluminum foil bags and sealed. A composition for promoting growth and anti-inflammation of egg chicks can be obtained.

4. The method for preparing a composition for promoting growth and reducing inflammation in egg-laying chicks according to claim 3, characterized in that: The stabilizer is either sodium alginate or carrageenan.

5. A method for preparing a composition for promoting growth and reducing inflammation in egg-laying chicks according to claim 3, characterized in that: The emulsifier is one of lecithin, glyceryl monostearate, or sucrose fatty acid ester.

6. The use of the composition of claim 1 or 2 or the composition of any one of claims 3 to 5 in the preparation of a composition for promoting growth and reducing inflammation in laying hens.

7. The application according to claim 6, characterized in that: The dosage of the traditional Chinese medicine composition or the traditional Chinese medicine preparation is as follows: add 1 to 2 kg of the composition per ton of feed, and feed once in the morning and once in the evening.