A centrifugal microfluidic chip and a nucleic acid extraction method

Abstract

This invention discloses a centrifugal microfluidic chip and a nucleic acid extraction method, specifically relating to the field of nucleic acid extraction technology. The method includes a pretreatment filtration module and a nucleic acid extraction and purification module. The sample solution undergoes primary and secondary filtration in the pretreatment filtration module to remove particulate impurities and protein precipitates, obtaining a clear sample supernatant. Nucleic acids in the supernatant are captured by a silica membrane in the nucleic acid extraction and purification module. Subsequently, a first washing solution and a second washing solution flow sequentially through the silica membrane to wash the adsorbed nucleic acids, removing residual impurities and reaction inhibitors. Finally, the eluent flows through the silica membrane, carrying the nucleic acids into the nucleic acid collection chamber, thereby obtaining the purified nucleic acid sample. This invention features a simple structure and reliable control, enabling sequential control of liquids in multiple chambers, effectively removing impurities and inhibitors from the sample, improving nucleic acid extraction efficiency and purity, and achieving a high degree of integration and automation in the nucleic acid extraction process for soil samples.

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